Rw3G025350

AAA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000279
Physical Location & Seq
Forward (+)
33745801 .. 33752718
6918 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G025350.1

Sequence Viewer

Length: 3438 bp
ATGGGGATGACGAGTAAGCTGAAACAGGAACAAGTTGCAGATACAAGAAGCCTTGTGGGTTTGGTATTCTCTTGGTCTATAAGGGATGTTCTCAATGAAAACCTTTATAGAAATCAGGTGCAGAGGATCCCCGACACATTTGTGACATTGACGAGTTACAAGAAATCATTCATTCCTTCACTTGTTGAGGAAACTCATGCTGATTTACTATCGAATATGTTGAATCTGTCACAAGCACCTACTTGTGAAATTCTGACAATCAAAGATTCAGATGGAACTCCTGGTGACTTGCTTTACGATGTAACATATCAGAAAGATACAGAAACTGATGAGAATCACAAAGGACTGATGTATGAGCCACAGGTTGGAGATATCATTGCCTTGACAAATGTTAGACCAAAATGCATTGATGATTTGAACAGGCCTCCAAGGTTCTATCTAATCGCTTATGTTGATAAAGCAAATGATATTGAAGAATTTCCTGATGATCTCCAGTTCAAAATACTATCATCGAAGCCTATCAACTATGGAGAACCAGACATGCATAAGAGCAAGAGAGAGACACTTTTTGCTGTCTATCTTATGAACTTGACAACAAATCTCCGTGTATGGAAGGCTTTGAACTCAGAGGGAAATACAAATATCATTAACAAAGTTCTGCAACCCAAGTCAGATGATGGGGATTCTTGTTCTGTTTGCTTCTCCAAAGAAAAATGCAGTGCTGGCATTTCTGCATTATGGCCTACAATTTGCTCTCAAAATCTAAATGAATCCCAAGAAGCTGCAGTTTTGAACTGTATCAGTTTGACTCAATGCCATCACCAGAATTCCGTAAAATTAATATGGGGTCCTCCTGGTACTGGCAAGACGAAGACAATGAGTCTGACACTCTTTGCTCTCCTTCAGTTAAAGTGCAGAACTCTCACATGTGCTCCCACCAATATTGCAGTGCTAGAAGTAGCAGCAAGACTCCGGAGATTGGTTAACCAGTCACTCGAGTATGGCAGGTATGGACTAGGAGATATAGTTCTCTTTGGTAACAAGAAGCGAATGAAGATTGACAATAATGATGATCTTCGTGATATATTTCTTGATCATCGTGTTAAAATTCTCATCAAGTGTTTGGTACCTTTATCTGGGTGGAAACATTTGTTAGAATCAATGATACATTTACTTGATGATCCTGAGGAACAATATTCTTTGTATTTGGAAAACAGAGCTAAAGAGCAGAAACAGAATGCACAAAAACATGAGGAGGATAATCAAAAACATAATGGAGAAGAGGATTATCCTTTGACATATGAGGAGTTTGTAAAGAATGAATTTGATTCAATCAGTCAGGAGCTGAAGGTTTGTATGGTAGATTTGTACACCCACTTACCAACTTGTTGCATTTCACTTAAAGTGGTGAAGGACATGGTCAGAGCTTTGGGTTTGCTCAAGTCTATTAAATCTTCATTGCATACTACTGTTGTTGCTAATGAAGGGTTGAAGTTAGTCTTCAAAGATTCCACAGTTCCAGGAAACATTGTTGGTTGCCTTACGCAGTTGAGAACAGAGTGTACAAACACACTGAAGTCGCTTCCTATTAACAAATATGCATTAATGAACTTCTGCCTAGAAAATGCTTGCTTAATATTCTGTACTGCATCAACTTCTTCCAAATTGCATGTTGTAGCAGGAACAAGACCATTGGAATTGTTGGTCATTGATGAAGCTGCTCAGCTTAAAGAATGTGAATCAGCAATTCCTTTACAACTATCTGGTCTCCGCCATGCTATCCTTGTAGGAGATGAGAGGCAACTCCCTGCCATGGTTCAAAGCAAGATTGCAGCAAGTGCTGATTTTGGAAGAAGTTTGTTTGGAAGGCTGGCAAAGCTGGGACACAAGAAGCACCTACTCAATGTCCAGTACAGGATGCATCCATCCATCAGTTTATTTCCGAAAAGGGAGTTCTACGACAACCAGATAGTAGATGGTCCAAATGTCAAAGAAAGAAGCTATGAGAGGTGCTTCCTCCTGGGAAAAATGTACCAATCCTATTCCTTCATAAATGTAGCCAATGGAAAAGAAGAATTTGATCACAGATTTAGTCAGAAAAATATGGTGGAGGTTGCTGTAGTCTCTGGGATAGTAGCAAGCCTTTATAAAGATTTTATTGGAACAAAGAAGAAAGTTAGTATTGGGGTCATATCACCATACAAGGCTCAAGTTTATGCAATTCAAGAGATACTCAGAAAATATACTGAAACTTCTGGTACTGGATTCTCCGTGAGTGTGCGATCTGTTGACGGGTTCCAAGGTGGTGAAGAGGATGTGATAATTATATCTACTGTCCGATGTAATGGGAAGGGGTCAGTTGGTTTCTTGTCGAACCGGCAAAGAGCAAATGTTGCATTAACACGTGCAAGGTATTGCCTTTGGATATTGGGGAATGGGTCAACTTTGATTACTAGTGACTCTGTTTGGAAGAAGCTAGTCCTTGATGCCAAGAAACGGAACTGTTTTTATAATGCTGATGAAGACAGCGACTTGGCCCAGGCTATTACAGCAGCCCTGCTCAAGCTTGACCAACTTCATTCTCTGCTTAATATTGACTCTATGCTGTTCAAAAACGCTATATGGAAGGTTTGCTTCACTGATGACTTTCGGAACTCCATAATAAAAATTAAAGACACTGTGATTCTTCGGGAAGTACTCGCGTTATTAACCAAGCTTTCGAGTGGATGTCGCCGACCTCACAAGGATAAAGGAAATTTAGTGTATGATGGGACTTCTGCTCAGCTGTTAGAGAAGTACAAAATCAAAGGGCACTTGAATCTCATTTGGACTGTAGATATTCTCCAGGAGAATGCACATTACGTCCAAGTTATGAAGTTTTGGGATATTTTGCCATTTTCTCATATACCAGAACTAGCAAAGCGTCTTGACATTGTTTTTGGAAATTTTACAGTGGATAAGATGAACCGCTGCAGGCACAAATGCATTGACAGGGATACTGTTGTTCCAATGAGATGGCCAGTGGTTTTCAGAAATTTTCCTGCAGCTGATCATGAGGAGTTCCTTTCAAAACCATTGTCTTCTTTCCGTATAACTACTAATGGAGAAACAGCAACTTCAACATATGGGGAGACAGCGAAAGCAGTAAAACCGATTATCTGTTCGAAAAGCAACGTCAATAAAAAAGAAAGATGGTTATGGAAAATAAAGGCTGAAGACAAAGTGAAGGAGTTCAGCACTTGCCTTGAAGCTGACCCTGTGGAGTTCTTTTCAAAACCATTATCATCACTAAGTATAGCAGAGAAGCCAGAAGCATCTACTTCCACTGACATATCCACAGCGATTGGATCATGCCTTTCCAGTACTCCTCAAAGTCGTTCAAAAAGCAATGCGAATAGAAGGAGATCATCATCAAAACTAAAAGTCCGTAAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12

Protein Analysis

1145

Amino Acids

129.14

Weight (kDa)

8.75

Isoelectric Point (pI)

39.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 80 - 212 8.1e-14 Domain of unknown function (DUF6469)
AAA_11 PF13086 254 - 609 6.2e-33 AAA domain
AAA_12 PF13087 617 - 812 2.2e-56 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000279)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65810 AT1G65810 AT1G65810 AT4G05540 AT5G37140 AT5G37150 AT5G37160 AT5G37165 AT5G52090
fragaria_vesca FvH4_1g27541 FvH4_1g27542 FvH4_2g08960 FvH4_2g08960 FvH4_2g08960 FvH4_2g08960 FvH4_3g00170 FvH4_3g00170 FvH4_3g00170 FvH4_5g05930 FvH4_5g05930
malus_domestica MD08G1077700.v1.1 MD08G1077900.v1.1 MD08G1078000.v1.1 MD08G1078100.v1.1 MD08G1078300.v1.1 MD09G1057800.v1.1 MD09G1281400.v1.1 MD11G1222900.v1.1 MD14G1146400.v1.1
prunus_persica Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G202100_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.3G013100_v2.0.a1
pyrus_communis pycom09g18900 pycom09g18910 pycom13g26720 pycom13g26730 pycom13g26760 pycom13g26770
rosa_chinensis RchiOBHm_Chr3g0488001 RchiOBHm_Chr3g0488011 RchiOBHm_Chr3g0488031 RchiOBHm_Chr3g0488061 RchiOBHm_Chr3g0488111 RchiOBHm_Chr3g0488201 RchiOBHm_Chr3g0488211 RchiOBHm_Chr3g0488221 RchiOBHm_Chr3g0488321 RchiOBHm_Chr3g0488431 RchiOBHm_Chr6g0264601 RchiOBHm_Chr6g0264641
rosa_laevigata RLG00000014240 RLG00000014241 RLG00000022910 RLG00000022949 RLG00000030376
rosa_multiflora Rmu_co8075388.1_g000001 Rmu_co8424869.1_g000001 Rmu_co8427783.1_g000001 Rmu_sc0000546.1_g000002 Rmu_sc0000546.1_g000015 Rmu_sc0011052.1_g000014 Rmu_sc0017772.1_g000004
rosa_roxburghii Rroxscaffold_175G00431980 Rroxscaffold_175G00431990 Rroxscaffold_1G00034600 Rroxscaffold_6G00394230 Rroxscaffold_6G00394270 Rroxscaffold_6G00394280 Rroxscaffold_6G00394350 Rroxscaffold_7G00203240 Rroxscaffold_7G00203270 Rroxscaffold_7G00203280 Rroxscaffold_7G00203300 Rroxscaffold_7G00205740
rosa_rugosa Rorug03G0238000 Rorug03G0238100 Rorug03G0238100 Rorug03G0238500 Rorug05G0155400 Rorug05G0356000 Rorug05G0463000 Rorug06G0015200 Rorug06G0015300 Rorug06G0015400 Rorug06G0015500 Rorug06G0015500
rosa_samantha Rh1DG088100 Rh2AG244800 Rh2BG187500 Rh2CG247300 Rh3BG322700 Rh3BG322800 Rh3BG323100 Rh3BG323900 Rh3BG324000 Rh3BG324300 Rh3CG321300 Rh3CG321400 Rh3CG321500 Rh3DG319400 Rh3DG319900 Rh3DG320100 Rh3DG320200 Rh6AG133700 Rh6AG133800 Rh6AG133900 Rh6CG129300 Rh6CG129800 Rh6CG130200 Rh6DG116500 Rh6DG117200 Rh7DG378000
rosa_wichuraiana Rw2G014710 Rw3G025340 Rw3G025350 Rw3G025360 Rw5G020630 Rw6G011590 Rw6G011600 Rw7G041380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2148, 2511
Acc36I ACCTGC 1 cut(s) 996
Acc65I GGTACC 1 cut(s) 1126
AccB1I GGYRCC 1 cut(s) 1126
AccB7I CCANNNNNTGG 1 cut(s) 365
AccII CGCG 1 cut(s) 2702
AccIII TCCGGA 1 cut(s) 972
AciI CCGC 2 cut(s) 1771, 2968
AclWI GGATC 4 cut(s) 121, 134, 1175, 3355
AcoI YGGCCR 1 cut(s) 3017
AcsI RAATTY 9 cut(s) 249, 476, 826, 1107, 1322, 2075, 2755, 2944, 3034
AcuI CTGAAG 4 cut(s) 887, 1367, 1595, 3234
AcvI CACGTG 1 cut(s) 2405
AfiI CCNNNNNNNGG 6 cut(s) 365, 860, 979, 1136, 1813, 2496
AflIII ACRYGT 2 cut(s) 926, 2402
AhdI GACNNNNNGTC 1 cut(s) 879
AhlI ACTAGT 1 cut(s) 2453
AjnI CCWGG 6 cut(s) 280, 853, 1519, 2019, 2538, 2844
AleI CACNNNNGTG 1 cut(s) 140
AloI GAACNNNNNNTCC 2 cut(s) 1011, 1043
Alw21I GWGCWC 1 cut(s) 934
Alw26I GTCTC 4 cut(s) 554, 1772, 2128, 3125
AlwI GGATC 4 cut(s) 121, 134, 1175, 3355
AlwNI CAGNNNCTG 2 cut(s) 326, 1345
Ama87I CYCGRG 1 cut(s) 995
Aor13HI TCCGGA 1 cut(s) 972
AoxI GGCC 4 cut(s) 422, 740, 2535, 3017
ApeKI GCWGC 7 cut(s) 782, 962, 1718, 1832, 2552, 2970, 3044
ApoI RAATTY 9 cut(s) 249, 476, 826, 1107, 1322, 2075, 2755, 2944, 3034
ArsI GACNNNNNNTTYG 4 cut(s) 2921, 2953, 3062, 3094
AseI ATTAAT 2 cut(s) 839, 1604
Asp700I GAANNNNTTC 3 cut(s) 167, 477, 3230
Asp718I GGTACC 1 cut(s) 1126
AspS9I GGNCC 3 cut(s) 848, 1979, 2536
AsuHPI GGTGA 5 cut(s) 296, 812, 1420, 2187, 2318
AsuII TTCGAA 1 cut(s) 3164
AvaI CYCGRG 1 cut(s) 995
AvaII GGWCC 2 cut(s) 848, 1979
AxyI CCTNAGG 1 cut(s) 1185
BaeGI GKGCMC 1 cut(s) 2814
BaeI ACNNNNGTAYC 2 cut(s) 2988, 3021
BalI TGGCCA 1 cut(s) 3019
BamHI GGATCC 1 cut(s) 126
BanI GGYRCC 1 cut(s) 1126
BbrPI CACGTG 1 cut(s) 2405
BbsI GAAGAC 5 cut(s) 878, 1492, 2529, 3072, 3222
Bbv12I GWGCWC 1 cut(s) 934
BbvI GCAGC 7 cut(s) 769, 974, 1705, 1844, 2564, 2957, 3056
BccI CCATC 9 cut(s) 266, 671, 825, 1933, 1937, 1970, 2762, 3009, 3186
BciT130I CCWGG 6 cut(s) 282, 855, 1521, 2021, 2540, 2846
BciVI GTATCC 1 cut(s) 2989
BclI TGATCA 3 cut(s) 1093, 2080, 3049
BcoDI GTCTC 4 cut(s) 554, 1772, 2128, 3125
BcuI ACTAGT 1 cut(s) 2453
BfaI CTAG 6 cut(s) 953, 1016, 1619, 2454, 2477, 2915
BfmI CTRYAG 5 cut(s) 783, 2118, 2832, 2971, 3042
BfuAI ACCTGC 1 cut(s) 996
BfuI GTATCC 1 cut(s) 2989
BisI GCNGC 7 cut(s) 783, 963, 1719, 1833, 2553, 2971, 3045
BlpI GCTNAGC 2 cut(s) 1722, 2781
BlsI GCNGC 7 cut(s) 784, 964, 1720, 1834, 2554, 2972, 3046
BmcAI AGTACT 2 cut(s) 2697, 3364
Bme1390I CCNGG 6 cut(s) 282, 855, 1521, 2021, 2540, 2846
Bme18I GGWCC 2 cut(s) 848, 1979
BmeRI GACNNNNNGTC 1 cut(s) 879
BmeT110I CYCGRG 1 cut(s) 995
BmgT120I GGNCC 3 cut(s) 848, 1979, 2536
BmiI GGNNCC 4 cut(s) 128, 849, 1128, 2297
BmrFI CCNGG 6 cut(s) 282, 855, 1521, 2021, 2540, 2846
BmsI GCATC 5 cut(s) 1658, 1908, 1930, 2476, 3323
BpiI GAAGAC 5 cut(s) 878, 1492, 2529, 3072, 3222
BpmI CTGGAG 2 cut(s) 476, 2828
Bpu1102I GCTNAGC 2 cut(s) 1722, 2781
Bpu14I TTCGAA 1 cut(s) 3164
BpuEI CTTGAG 3 cut(s) 1424, 2193, 2546
BsaAI YACGTR 1 cut(s) 2405
BsaBI GATNNNNATC 2 cut(s) 333, 3409
BsaI GGTCTC 1 cut(s) 1772
BsaJI CCNNGG 5 cut(s) 428, 1812, 2020, 2299, 2538
BsaWI WCCGGW 1 cut(s) 972
BsaXI ACNNNNNCTCC 4 cut(s) 916, 946, 1011, 1041
Bsc4I CCNNNNNNNGG 6 cut(s) 365, 860, 979, 1136, 1813, 2496
Bse118I RCCGGY 1 cut(s) 2376
Bse1I ACTGG 7 cut(s) 493, 865, 988, 1909, 2266, 3020, 3360
Bse21I CCTNAGG 1 cut(s) 1185
Bse3DI GCAATG 3 cut(s) 375, 1457, 3394
Bse8I GATNNNNATC 2 cut(s) 333, 3409
BseAI TCCGGA 1 cut(s) 972
BseBI CCWGG 6 cut(s) 282, 855, 1521, 2021, 2540, 2846
BseDI CCNNGG 5 cut(s) 428, 1812, 2020, 2299, 2538
BseGI GGATG 7 cut(s) 12, 91, 1921, 1923, 1925, 2320, 2732
BseJI GATNNNNATC 2 cut(s) 333, 3409
BseLI CCNNNNNNNGG 6 cut(s) 365, 860, 979, 1136, 1813, 2496
BseMI GCAATG 3 cut(s) 375, 1457, 3394
BseMII CTCAG 5 cut(s) 639, 1176, 1736, 2248, 2795
BseNI ACTGG 7 cut(s) 493, 865, 988, 1909, 2266, 3020, 3360
BseRI GAGGAG 4 cut(s) 1268, 1319, 3071, 3357
BseSI GKGCMC 1 cut(s) 2814
BseXI GCAGC 7 cut(s) 769, 974, 1705, 1844, 2564, 2957, 3056
BseYI CCCAGC 1 cut(s) 1879
BsgI GTGCAG 2 cut(s) 140, 934
Bsh1236I CGCG 1 cut(s) 2702
BshFI GGCC 4 cut(s) 424, 742, 2537, 3019
BshNI GGYRCC 1 cut(s) 1126
BsiHKAI GWGCWC 1 cut(s) 934
BsiHKCI CYCGRG 1 cut(s) 995
BsiSI CCGG 2 cut(s) 973, 2377
BslFI GGGAC 2 cut(s) 1896, 2785
BslI CCNNNNNNNGG 6 cut(s) 365, 860, 979, 1136, 1813, 2496
BsmAI GTCTC 4 cut(s) 554, 1772, 2128, 3125
BsmFI GGGAC 2 cut(s) 1896, 2785
BsmI GAATGC 2 cut(s) 1243, 2857
BsnI GGCC 4 cut(s) 424, 742, 2537, 3019
Bso31I GGTCTC 1 cut(s) 1772
BsoBI CYCGRG 1 cut(s) 995
Bsp119I TTCGAA 1 cut(s) 3164
Bsp1286I GDGCHC 2 cut(s) 934, 2814
Bsp13I TCCGGA 1 cut(s) 972
Bsp1407I TGTACA 2 cut(s) 1368, 1562
Bsp1720I GCTNAGC 2 cut(s) 1722, 2781
Bsp19I CCATGG 1 cut(s) 1812
BspACI CCGC 2 cut(s) 1771, 2968
BspANI GGCC 4 cut(s) 424, 742, 2537, 3019
BspCNI CTCAG 5 cut(s) 638, 1177, 1735, 2247, 2794
BspEI TCCGGA 1 cut(s) 972
BspFNI CGCG 1 cut(s) 2702
BspHI TCATGA 1 cut(s) 3052
BspLI GGNNCC 4 cut(s) 128, 849, 1128, 2297
BspMAI CTGCAG 3 cut(s) 787, 2975, 3046
BspMI ACCTGC 1 cut(s) 996
BspPI GGATC 4 cut(s) 121, 134, 1175, 3355
BspT104I TTCGAA 1 cut(s) 3164
BspT107I GGYRCC 1 cut(s) 1126
BspTNI GGTCTC 1 cut(s) 1772
BsrDI GCAATG 3 cut(s) 375, 1457, 3394
BsrFI RCCGGY 1 cut(s) 2376
BsrGI TGTACA 2 cut(s) 1368, 1562
BsrI ACTGG 7 cut(s) 493, 865, 988, 1909, 2266, 3020, 3360
BssAI RCCGGY 1 cut(s) 2376
BssECI CCNNGG 5 cut(s) 428, 1812, 2020, 2299, 2538
BssT1I CCWWGG 3 cut(s) 428, 1812, 2299
Bst2UI CCWGG 6 cut(s) 282, 855, 1521, 2021, 2540, 2846
Bst4CI ACNGT 9 cut(s) 797, 1471, 1516, 2335, 2504, 2680, 2833, 2953, 3001
Bst6I CTCTTC 2 cut(s) 1275, 2304
BstAPI GCANNNNNTGC 2 cut(s) 1838, 2393
BstAUI TGTACA 2 cut(s) 1368, 1562
BstBAI YACGTR 1 cut(s) 2405
BstBI TTCGAA 1 cut(s) 3164
BstC8I GCNNGC 5 cut(s) 724, 1630, 1872, 2140, 2975
BstDEI CTNAG 6 cut(s) 625, 1185, 1722, 2234, 2781, 3290
BstDSI CCRYGG 1 cut(s) 1812
BstF5I GGATG 7 cut(s) 12, 91, 1921, 1923, 1925, 2320, 2732
BstFNI CGCG 1 cut(s) 2702
BstMAI GTCTC 4 cut(s) 554, 1772, 2128, 3125
BstMWI GCNNNNNNNGC 5 cut(s) 723, 1838, 1876, 2393, 2549
BstNI CCWGG 6 cut(s) 282, 855, 1521, 2021, 2540, 2846
BstNSI RCATGY 3 cut(s) 544, 930, 1673
BstSCI CCNGG 6 cut(s) 280, 853, 1519, 2019, 2538, 2844
BstSFI CTRYAG 5 cut(s) 783, 2118, 2832, 2971, 3042
BstSLI GKGCMC 1 cut(s) 2814
BstUI CGCG 1 cut(s) 2702
BstV1I GCAGC 7 cut(s) 769, 974, 1705, 1844, 2564, 2957, 3056
BstV2I GAAGAC 5 cut(s) 878, 1492, 2529, 3072, 3222
BstX2I RGATCY 1 cut(s) 126
BstXI CCANNNNNNTGG 1 cut(s) 3015
BstYI RGATCY 1 cut(s) 126
Bsu36I CCTNAGG 1 cut(s) 1185
BsuI GTATCC 1 cut(s) 2989
BsuRI GGCC 4 cut(s) 424, 742, 2537, 3019
BtgI CCRYGG 1 cut(s) 1812
BtsCI GGATG 7 cut(s) 12, 91, 1921, 1923, 1925, 2320, 2732
BtsI GCAGTG 2 cut(s) 724, 954
BtsIMutI CAGTG 8 cut(s) 724, 954, 1571, 2637, 2676, 2958, 3027, 3324
BveI ACCTGC 1 cut(s) 996
Cac8I GCNNGC 5 cut(s) 724, 1630, 1872, 2140, 2975
CaiI CAGNNNCTG 2 cut(s) 326, 1345
CciI TCATGA 1 cut(s) 3052
Cfr10I RCCGGY 1 cut(s) 2376
Cfr13I GGNCC 3 cut(s) 848, 1979, 2536
CseI GACGC 1 cut(s) 2912
CspCI CAANNNNNGTGG 4 cut(s) 925, 960, 3325, 3360
DdeI CTNAG 6 cut(s) 625, 1185, 1722, 2234, 2781, 3290
DriI GACNNNNNGTC 1 cut(s) 879
EaeI YGGCCR 1 cut(s) 3017
Eam1104I CTCTTC 2 cut(s) 1275, 2304
Eam1105I GACNNNNNGTC 1 cut(s) 879
EarI CTCTTC 2 cut(s) 1275, 2304
EciI GGCGGA 1 cut(s) 1760
Eco130I CCWWGG 3 cut(s) 428, 1812, 2299
Eco147I AGGCCT 1 cut(s) 424
Eco31I GGTCTC 1 cut(s) 1772
Eco32I GATATC 1 cut(s) 373
Eco47I GGWCC 2 cut(s) 848, 1979
Eco57I CTGAAG 4 cut(s) 887, 1367, 1595, 3234
Eco72I CACGTG 1 cut(s) 2405
Eco81I CCTNAGG 1 cut(s) 1185
Eco88I CYCGRG 1 cut(s) 995
EcoO109I RGGNCCY 1 cut(s) 848
EcoRI GAATTC 1 cut(s) 826
EcoRII CCWGG 6 cut(s) 280, 853, 1519, 2019, 2538, 2844
EcoRV GATATC 1 cut(s) 373
EcoT14I CCWWGG 3 cut(s) 428, 1812, 2299
EcoT22I ATGCAT 5 cut(s) 407, 546, 1603, 1923, 2987
ErhI CCWWGG 3 cut(s) 428, 1812, 2299
FalI AAGNNNNNCTT 4 cut(s) 1484, 1516, 2618, 2650
FaqI GGGAC 2 cut(s) 1896, 2785
FauNDI CATATG 2 cut(s) 1300, 3124
FbaI TGATCA 3 cut(s) 1093, 2080, 3049
Fnu4HI GCNGC 7 cut(s) 783, 963, 1719, 1833, 2553, 2971, 3045
FokI GGATG 7 cut(s) 19, 98, 1908, 1912, 1930, 2327, 2739
Fsp4HI GCNGC 7 cut(s) 783, 963, 1719, 1833, 2553, 2971, 3045
FspBI CTAG 6 cut(s) 953, 1016, 1619, 2454, 2477, 2915
GluI GCNGC 7 cut(s) 783, 963, 1719, 1833, 2553, 2971, 3045
GsaI CCCAGC 1 cut(s) 1883
GsuI CTGGAG 2 cut(s) 476, 2828
HaeIII GGCC 4 cut(s) 424, 742, 2537, 3019
HapII CCGG 2 cut(s) 973, 2377
HgaI GACGC 1 cut(s) 2912
HincII GTYRAC 3 cut(s) 985, 2290, 2442
HindII GTYRAC 3 cut(s) 985, 2290, 2442
HindIII AAGCTT 2 cut(s) 2564, 2714
HpaI GTTAAC 1 cut(s) 985
HpaII CCGG 2 cut(s) 973, 2377
HphI GGTGA 5 cut(s) 296, 812, 1420, 2187, 2318
Hpy166II GTNNAC 5 cut(s) 985, 1371, 1563, 2290, 2442
Hpy8I GTNNAC 5 cut(s) 985, 1371, 1563, 2290, 2442
HpyCH4III ACNGT 9 cut(s) 797, 1471, 1516, 2335, 2504, 2680, 2833, 2953, 3001
HpyCH4IV ACGT 3 cut(s) 2404, 2862, 3174
HpyF10VI GCNNNNNNNGC 5 cut(s) 723, 1838, 1876, 2393, 2549
HpyF3I CTNAG 6 cut(s) 625, 1185, 1722, 2234, 2781, 3290
HpySE526I ACGT 3 cut(s) 2404, 2862, 3174
Kpn2I TCCGGA 1 cut(s) 972
KpnI GGTACC 1 cut(s) 1130
Ksp22I TGATCA 3 cut(s) 1093, 2080, 3049
KspAI GTTAAC 1 cut(s) 985
LmnI GCTCC 2 cut(s) 937, 1342
Lsp1109I GCAGC 7 cut(s) 769, 974, 1705, 1844, 2564, 2957, 3056
LweI GCATC 5 cut(s) 1658, 1908, 1930, 2476, 3323
MaeI CTAG 6 cut(s) 953, 1016, 1619, 2454, 2477, 2915
MaeII ACGT 3 cut(s) 2404, 2862, 3174
MaeIII GTNAC 8 cut(s) 142, 155, 228, 284, 301, 990, 1037, 2456
MflI RGATCY 1 cut(s) 126
MhlI GDGCHC 2 cut(s) 934, 2814
MlsI TGGCCA 1 cut(s) 3019
MluNI TGGCCA 1 cut(s) 3019
MlyI GAGTC 5 cut(s) 802, 889, 963, 2453, 2591
MmeI TCCRAC 1 cut(s) 346
Mox20I TGGCCA 1 cut(s) 3019
Mph1103I ATGCAT 5 cut(s) 407, 546, 1603, 1923, 2987
MroI TCCGGA 1 cut(s) 972
MroXI GAANNNNTTC 3 cut(s) 167, 477, 3230
MscI TGGCCA 1 cut(s) 3019
MslI CAYNNNNRTG 1 cut(s) 140
Msp20I TGGCCA 1 cut(s) 3019
MspA1I CMGCKG 3 cut(s) 2785, 2970, 3047
MspI CCGG 2 cut(s) 973, 2377
MspR9I CCNGG 6 cut(s) 282, 855, 1521, 2021, 2540, 2846
Mva1269I GAATGC 2 cut(s) 1243, 2857
MvaI CCWGG 6 cut(s) 282, 855, 1521, 2021, 2540, 2846
MvnI CGCG 1 cut(s) 2702
MwoI GCNNNNNNNGC 5 cut(s) 723, 1838, 1876, 2393, 2549
NcoI CCATGG 1 cut(s) 1812
NdeI CATATG 2 cut(s) 1300, 3124
NlaIV GGNNCC 4 cut(s) 128, 849, 1128, 2297
NmuCI GTSAC 5 cut(s) 142, 228, 284, 990, 2456
NsiI ATGCAT 5 cut(s) 407, 546, 1603, 1923, 2987
NspI RCATGY 3 cut(s) 544, 930, 1673
NspV TTCGAA 1 cut(s) 3164
OliI CACNNNNGTG 1 cut(s) 140
PaeR7I CTCGAG 1 cut(s) 995
PagI TCATGA 1 cut(s) 3052
PceI AGGCCT 1 cut(s) 424
PciI ACATGT 1 cut(s) 926
PctI GAATGC 2 cut(s) 1243, 2857
PdmI GAANNNNTTC 3 cut(s) 167, 477, 3230
PflFI GACNNNGTC 1 cut(s) 1418
PflMI CCANNNNNTGG 1 cut(s) 365
PfoI TCCNGGA 2 cut(s) 1519, 2844
PkrI GCNGC 7 cut(s) 784, 964, 1720, 1834, 2554, 2972, 3046
PleI GAGTC 5 cut(s) 802, 888, 963, 2453, 2591
PmaCI CACGTG 1 cut(s) 2405
PmlI CACGTG 1 cut(s) 2405
PpsI GAGTC 5 cut(s) 802, 888, 963, 2453, 2591
Ppu21I YACGTR 1 cut(s) 2405
PpuMI RGGWCCY 1 cut(s) 848
PscI ACATGT 1 cut(s) 926
PshBI ATTAAT 2 cut(s) 839, 1604
PsiI TTATAA 2 cut(s) 2148, 2511
Psp5II RGGWCCY 1 cut(s) 848
Psp6I CCWGG 6 cut(s) 280, 853, 1519, 2019, 2538, 2844
PspCI CACGTG 1 cut(s) 2405
PspFI CCCAGC 1 cut(s) 1879
PspGI CCWGG 6 cut(s) 280, 853, 1519, 2019, 2538, 2844
PspN4I GGNNCC 4 cut(s) 128, 849, 1128, 2297
PspPI GGNCC 3 cut(s) 848, 1979, 2536
PspPPI RGGWCCY 1 cut(s) 848
PspXI VCTCGAGB 1 cut(s) 995
PsrI GAACNNNNNNTAC 2 cut(s) 1546, 1578
PstI CTGCAG 3 cut(s) 787, 2975, 3046
PstNI CAGNNNCTG 2 cut(s) 326, 1345
PsuI RGATCY 1 cut(s) 126
PsyI GACNNNGTC 1 cut(s) 1418
PvuII CAGCTG 2 cut(s) 2785, 3047
RseI CAYNNNNRTG 1 cut(s) 140
SatI GCNGC 7 cut(s) 783, 963, 1719, 1833, 2553, 2971, 3045
Sau96I GGNCC 3 cut(s) 848, 1979, 2536
ScaI AGTACT 2 cut(s) 2697, 3364
SchI GAGTC 5 cut(s) 802, 889, 963, 2453, 2591
ScrFI CCNGG 6 cut(s) 282, 855, 1521, 2021, 2540, 2846
SduI GDGCHC 2 cut(s) 934, 2814
SfaNI GCATC 5 cut(s) 1658, 1908, 1930, 2476, 3323
SfcI CTRYAG 5 cut(s) 783, 2118, 2832, 2971, 3042
Sfr274I CTCGAG 1 cut(s) 995
SfuI TTCGAA 1 cut(s) 3164
SinI GGWCC 2 cut(s) 848, 1979
SlaI CTCGAG 1 cut(s) 995
SmiMI CAYNNNNRTG 1 cut(s) 140
SmlI CTYRAG 4 cut(s) 995, 1439, 2208, 2561
SmoI CTYRAG 4 cut(s) 995, 1439, 2208, 2561
SpeI ACTAGT 1 cut(s) 2453
SseBI AGGCCT 1 cut(s) 424
SsiI CCGC 2 cut(s) 1771, 2968
SspI AATATT 4 cut(s) 943, 1196, 1638, 2593
SspMI CTAG 6 cut(s) 953, 1016, 1619, 2454, 2477, 2915
StuI AGGCCT 1 cut(s) 424
StyD4I CCNGG 6 cut(s) 280, 853, 1519, 2019, 2538, 2844
StyI CCWWGG 3 cut(s) 428, 1812, 2299
TaaI ACNGT 9 cut(s) 797, 1471, 1516, 2335, 2504, 2680, 2833, 2953, 3001
TaiI ACGT 3 cut(s) 2407, 2865, 3177
TaqI TCGA 6 cut(s) 212, 512, 996, 2372, 2720, 3164
TatI WGTACW 7 cut(s) 1368, 1562, 1643, 1911, 2695, 2796, 3362
TscAI CASTG 8 cut(s) 724, 954, 1578, 2644, 2683, 2958, 3027, 3331
TseFI GTSAC 5 cut(s) 142, 228, 284, 990, 2456
TseI GCWGC 7 cut(s) 782, 962, 1718, 1832, 2552, 2970, 3044
Tsp45I GTSAC 5 cut(s) 142, 228, 284, 990, 2456
TspGWI ACGGA 6 cut(s) 593, 820, 2260, 2512, 3077, 3416
TspRI CASTG 8 cut(s) 724, 954, 1578, 2644, 2683, 2958, 3027, 3331
Tth111I GACNNNGTC 1 cut(s) 1418
Van91I CCANNNNNTGG 1 cut(s) 365
VpaK11BI GGWCC 2 cut(s) 848, 1979
VspI ATTAAT 2 cut(s) 839, 1604
XapI RAATTY 9 cut(s) 249, 476, 826, 1107, 1322, 2075, 2755, 2944, 3034
XceI RCATGY 3 cut(s) 544, 930, 1673
XcmI CCANNNNNNNNNTGG 1 cut(s) 1973
XhoI CTCGAG 1 cut(s) 995
XmnI GAANNNNTTC 3 cut(s) 167, 477, 3230
XspI CTAG 6 cut(s) 953, 1016, 1619, 2454, 2477, 2915
ZrmI AGTACT 2 cut(s) 2697, 3364
Zsp2I ATGCAT 5 cut(s) 407, 546, 1603, 1923, 2987
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.