Rw3G025340

AAA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000279
Physical Location & Seq
Forward (+)
33705297 .. 33712529
7233 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G025340.1

Sequence Viewer

Length: 3285 bp
ATGGGGATGAAGAGTAAGCTGAAACAGGAACAAGTTACAGATACTAGCAGCCTAGTGGGTTTGGTATTCTCTTGGTCTATTTCGGATGTTCTCAATGAAAATCTTTACAGAAATCAGGTGCAGAGGATCCCCGAGACATTTGTGTCATTGACGAGTTACAAGAAATCTTTCATTCCTTCACTTGTTGAGGAAACTCATGCTGATTTACTATCAAATATGAAGACTCTGTCACATGCACCTACTTGTGAAATTTTGACAATCGTAGATTCAGCTGAACATCCTGGTGACTTGTTTTACGATATAATGTATAAGAGAGATACAGAAACTGATGGGAATCACAAAGGACCGATGTATGAGCCACAGGTTGGAGATATCATTGCCTTAACAAATGTTAGACCAAAATGCATTAATGATTTGAACAGGCCTCCAAGGTTCTATCTAATCGCTTATGTTGATAAAGCAAATGATATTGAAGAATTTCCTGATGATCTCCAGTTCAAAATACTATCATCGAAGCCTATCAACTATGGAGAACCAGACATGCAGAAGAGCAAGAGAGAGACACTTTTTGCTGTCTATCTTATGAACTTGACAACAAATCTCCGTGTATGGAAGGCTTTGAACTCAGAAGAGGGAAATACAAATATCATTAGCAAAGTTCTGCAACCCAAGTCAGATGATGGGGATTCTTGTTCAGTTTGGTTATCCAAATACAAATGCAATGCTGGCATTTCTGCTATATGGCCTACAATTTGCTCTCAAAATCTAAATGAATCCCAAGAAGCTGCACTGTTGAACTGTATCAGTTTGACTCAATGCCATCACCAGAATTCTGTAAAATTAATATGGGGTCCTCCTGGTACCGGCAAGACAAAGACTATGAGTGTGACACTCTTTTCTCTCTTTCAGTTAAAGTGCAGAACTCTGACATGTGCTCCTACCAATATTGCGGTGCTAGAAGTAGCAGCAAGACTCCGGAGATTGGTTAACCAGTCACTCGAGTATTGCAGGTATGGACTAGGGGATATAGTTCTCTTTGGTAACAAGAAGCGAATGAAGATTGACAATGATAATCTTCGTGACATATTTCTTGATCATCGTGTTAAAATCCTCATCAAGTGTTTGGTCCCTTTATCTGGGTGGAAACATTTGTTAGAATCAATGATACATTTACTTGATGATCCTGAGGAACAATATTCTTTGTATTTGGAAAAGAGAGCTGAAAAGCAGAAACAGAATGCACAAAAAAGTGAGGAGGATAATCGAAAACATAATGGAGATGCAGATTATCCTTTGACATTTGAGGAGTTTGTAAAGAATGAATTTGATTCAATCAGTCAGGAGCTGAAGATTTGTATGGTACATTTATACACCCACTTACCAACTTCTTGCATTTCACTCAAAGTGGTGAAGGACATGGTCAGAGCTTTGGGTTTGCTCAACTCTATTGAATCTTCATTGCATACTATTGGTGTTGCTAATGAAGGGTTGAAGTTAATCAAAGATTTCAAAGTTCCAGGAAGCGTTGTTGGTTGCCTTATGCAGTTGAGAACAGACTGTACAAAAACACTAAAGTCGCTTCCAATGGAATTCTCTGTTCCTATTAATAAATATGCATTAATGAACTTCTGCCTAGAAAATGCTTGCTTAATATTCTGTACTGCATCAACTTCTTCCAAATTGCATGTTGTAGCAGGAAAAAGACCATTGGAATTGTTGGTCATTGATGAAGCTGCTCAACTTAAAGAATGTGAATCAGCAATTCCTTTACAACTATCTGGTCTCCGCCATGCTATCCTTGTAGGAGATGAGAGGCAACTCCCTGCCATGGTTCAAAGCAAGATTGCAGCAAGTGCTGATTTTGGAAGAAGTTTGTTTGGAAGGCTGGCAAAGCTGGGACACAAGAAGCACCTACTCAATGTCCAGTACAGGATGCATCCATCCATCAGTTTATTTCCGAAAAGGGAGTTCTACGACAACCAGATAGTAGATGGTCCAAATGTCAAAGAAAGAAGCTATGAGAGGTGCTTCCTCCTGGGAAAAATGTACCAATCCTATTCCTTCATAAATGTAGCCAATGGAAAAGAAGAATTTGATCACAGATTTAGTCAGAAAAATATGGTGGAGGTTGCTGTAGTCTCTGGGATAGTAGCAAGCCTTTATAAAGATTTTATTGGAACAAAGAAGAAAGTTAGTATTGGGGTCATATCACCATACAAGGCTCAAGTTTATGCAATTCAAGAGATACTCAGAAAATATGCTGAAACTTCTGATACTGGATTCTCCGTAAGTGTGCGATCTGTTGATGGGTTCCAAGGTGGTGAAGAAGATGTGATAATTATATCCACTGTCCGATGTAATGGGAACGGGTCAGTTGGTTTCTTGTCGAACCGTCAAAGAGCAAATGTTGCCTTAACACGTGCAAGGTATTGCCTTTGGATATTGGGGAATGCGTCTACTTTGATTGCTAGTGACACTGTTTGGAAGAAGCTAGTCCTTGATGCCAAGAAACGGAACTGTTTTTATAATGCTGATGAAGACAGCAAGTTGGTTCAGGTTATTACAGCTGCCCTGCTGGAGCTTGACCAACTTCATTCTCTGCTTAATATCGACTCTATGCTGTTCAAAAACGCCATATGGAAGGTTTGCTTCACTGATGACTTTCGGAACTCCATAATAAAAATTAAAGACACTGTGATTCTTCGGGAAGTACTCGCGTTATTAACCAAGCTTTCGAGTGGATGGCGCCGACCTCACAAGGATAAAGGAAATTTAGTGTATGATGGGACTTCTGCTCAGCTGTTAGAGAAGTATAAAATCAAAGGGCACTTGAATCTCATTTGGACTGTAGATATTCTCCAGGAGAATGCACATTACGTCCAAGTTATGAAGTTTTTGGATATTTTGCCATTTTCTCATACACCAGAACTAGCAAAGCGTCTTGACATTGTTTTTGGGAATTTTACAGTGGACAAGATGAACCGCTGCCGTCACAAATGCATTGACAGGGATACTGTCGTTCCAATGAGATGGCCGGTGGTCTTGGGCAATTTCCCTATAGCTGATCATGAGGAGTTCCTTTCAAAACCATTGCCTTCTTTCAGTATAACAACTAATCGAGAAACAGCAACTTCAACATATGGGGAGACAGCGAAAGCAGTAAAACCAATTATCCCTTCGAGAAGCATTGTCAATCAAAAAGAAAGATTGGCTAAGACTACGGACGACCATGGTGCATCTTTGACTTTATCAAAAATGTCTATTGACACGGTATACAAGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12

Protein Analysis

1094

Amino Acids

123.67

Weight (kDa)

8.68

Isoelectric Point (pI)

37.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 80 - 209 5.1e-13 Domain of unknown function (DUF6469)
AAA_11 PF13086 255 - 614 6e-32 AAA domain
AAA_12 PF13087 622 - 818 8.9e-57 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000279)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65810 AT1G65810 AT1G65810 AT4G05540 AT5G37140 AT5G37150 AT5G37160 AT5G37165 AT5G52090
fragaria_vesca FvH4_1g27541 FvH4_1g27542 FvH4_2g08960 FvH4_2g08960 FvH4_2g08960 FvH4_2g08960 FvH4_3g00170 FvH4_3g00170 FvH4_3g00170 FvH4_5g05930 FvH4_5g05930
malus_domestica MD08G1077700.v1.1 MD08G1077900.v1.1 MD08G1078000.v1.1 MD08G1078100.v1.1 MD08G1078300.v1.1 MD09G1057800.v1.1 MD09G1281400.v1.1 MD11G1222900.v1.1 MD14G1146400.v1.1
prunus_persica Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G202100_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.3G013100_v2.0.a1
pyrus_communis pycom09g18900 pycom09g18910 pycom13g26720 pycom13g26730 pycom13g26760 pycom13g26770
rosa_chinensis RchiOBHm_Chr3g0488001 RchiOBHm_Chr3g0488011 RchiOBHm_Chr3g0488031 RchiOBHm_Chr3g0488061 RchiOBHm_Chr3g0488111 RchiOBHm_Chr3g0488201 RchiOBHm_Chr3g0488211 RchiOBHm_Chr3g0488221 RchiOBHm_Chr3g0488321 RchiOBHm_Chr3g0488431 RchiOBHm_Chr6g0264601 RchiOBHm_Chr6g0264641
rosa_laevigata RLG00000014240 RLG00000014241 RLG00000022910 RLG00000022949 RLG00000030376
rosa_multiflora Rmu_co8075388.1_g000001 Rmu_co8424869.1_g000001 Rmu_co8427783.1_g000001 Rmu_sc0000546.1_g000002 Rmu_sc0000546.1_g000015 Rmu_sc0011052.1_g000014 Rmu_sc0017772.1_g000004
rosa_roxburghii Rroxscaffold_175G00431980 Rroxscaffold_175G00431990 Rroxscaffold_1G00034600 Rroxscaffold_6G00394230 Rroxscaffold_6G00394270 Rroxscaffold_6G00394280 Rroxscaffold_6G00394350 Rroxscaffold_7G00203240 Rroxscaffold_7G00203270 Rroxscaffold_7G00203280 Rroxscaffold_7G00203300 Rroxscaffold_7G00205740
rosa_rugosa Rorug03G0238000 Rorug03G0238100 Rorug03G0238100 Rorug03G0238500 Rorug05G0155400 Rorug05G0356000 Rorug05G0463000 Rorug06G0015200 Rorug06G0015300 Rorug06G0015400 Rorug06G0015500 Rorug06G0015500
rosa_samantha Rh1DG088100 Rh2AG244800 Rh2BG187500 Rh2CG247300 Rh3BG322700 Rh3BG322800 Rh3BG323100 Rh3BG323900 Rh3BG324000 Rh3BG324300 Rh3CG321300 Rh3CG321400 Rh3CG321500 Rh3DG319400 Rh3DG319900 Rh3DG320100 Rh3DG320200 Rh6AG133700 Rh6AG133800 Rh6AG133900 Rh6CG129300 Rh6CG129800 Rh6CG130200 Rh6DG116500 Rh6DG117200 Rh7DG378000
rosa_wichuraiana Rw2G014710 Rw3G025340 Rw3G025350 Rw3G025360 Rw5G020630 Rw6G011590 Rw6G011600 Rw7G041380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2163, 2526
AasI GACNNNNNNGTC 1 cut(s) 142
Acc36I ACCTGC 1 cut(s) 999
Acc65I GGTACC 1 cut(s) 860
AccB1I GGYRCC 2 cut(s) 860, 2745
AccB7I CCANNNNNTGG 1 cut(s) 365
AccI GTMKAC 2 cut(s) 2456, 3273
AccII CGCG 1 cut(s) 2717
AccIII TCCGGA 1 cut(s) 975
AciI CCGC 3 cut(s) 950, 1786, 2983
AclWI GGATC 3 cut(s) 121, 134, 1175
AcoI YGGCCR 1 cut(s) 3032
AcsI RAATTY 8 cut(s) 249, 476, 829, 1322, 1589, 2090, 2770, 2959
AcuI CTGAAG 1 cut(s) 1367
AcvI CACGTG 1 cut(s) 2420
AcyI GRCGYC 1 cut(s) 2746
AfaI GTAC 7 cut(s) 862, 1362, 1561, 1660, 1928, 2048, 2712
AfiI CCNNNNNNNGG 6 cut(s) 365, 863, 982, 1136, 1828, 2511
AflIII ACRYGT 2 cut(s) 929, 2417
AjnI CCWGG 5 cut(s) 280, 856, 1516, 2034, 2859
AjuI GAANNNNNNNTTGG 2 cut(s) 3160, 3192
Alw21I GWGCWC 1 cut(s) 937
Alw26I GTCTC 5 cut(s) 128, 554, 1787, 2143, 3140
AlwI GGATC 3 cut(s) 121, 134, 1175
AlwNI CAGNNNCTG 2 cut(s) 326, 1345
Ama87I CYCGRG 2 cut(s) 131, 998
Aor13HI TCCGGA 1 cut(s) 975
AoxI GGCC 3 cut(s) 422, 743, 3032
ApeKI GCWGC 7 cut(s) 48, 785, 965, 1733, 1847, 2567, 2985
ApoI RAATTY 8 cut(s) 249, 476, 829, 1322, 1589, 2090, 2770, 2959
ArsI GACNNNNNNTTYG 2 cut(s) 2936, 2968
AseI ATTAAT 4 cut(s) 408, 842, 1605, 1619
Asp700I GAANNNNTTC 2 cut(s) 167, 477
Asp718I GGTACC 1 cut(s) 860
AspLEI GCGC 1 cut(s) 2748
AspS9I GGNCC 4 cut(s) 344, 851, 1126, 1994
AsuHPI GGTGA 5 cut(s) 296, 815, 1420, 2202, 2333
AvaI CYCGRG 2 cut(s) 131, 998
AvaII GGWCC 4 cut(s) 344, 851, 1126, 1994
AxyI CCTNAGG 1 cut(s) 1185
BaeGI GKGCMC 1 cut(s) 2829
BaeI ACNNNNGTAYC 2 cut(s) 3003, 3036
BamHI GGATCC 1 cut(s) 126
BanI GGYRCC 2 cut(s) 860, 2745
BbrPI CACGTG 1 cut(s) 2420
BbsI GAAGAC 2 cut(s) 227, 2544
Bbv12I GWGCWC 1 cut(s) 937
BbvI GCAGC 7 cut(s) 60, 772, 977, 1720, 1859, 2554, 2972
BceAI ACGGC 1 cut(s) 2973
BcgI CGANNNNNNTGC 2 cut(s) 3215, 3249
BciT130I CCWGG 5 cut(s) 282, 858, 1518, 2036, 2861
BciVI GTATCC 1 cut(s) 3004
BclI TGATCA 3 cut(s) 1093, 2095, 3064
BcoDI GTCTC 5 cut(s) 128, 554, 1787, 2143, 3140
BfaI CTAG 8 cut(s) 45, 53, 956, 1019, 1634, 2469, 2492, 2930
BfmI CTRYAG 3 cut(s) 2133, 2847, 3057
BfoI RGCGCY 1 cut(s) 2749
BfuAI ACCTGC 1 cut(s) 999
BfuI GTATCC 1 cut(s) 3004
BisI GCNGC 7 cut(s) 49, 786, 966, 1734, 1848, 2568, 2986
BlpI GCTNAGC 1 cut(s) 2796
BlsI GCNGC 7 cut(s) 50, 787, 967, 1735, 1849, 2569, 2987
BmcAI AGTACT 1 cut(s) 2712
Bme1390I CCNGG 5 cut(s) 282, 858, 1518, 2036, 2861
Bme18I GGWCC 4 cut(s) 344, 851, 1126, 1994
BmeT110I CYCGRG 2 cut(s) 131, 998
BmgT120I GGNCC 4 cut(s) 344, 851, 1126, 1994
BmiI GGNNCC 6 cut(s) 128, 852, 862, 1128, 2312, 2747
BmrFI CCNGG 5 cut(s) 282, 858, 1518, 2036, 2861
BmsI GCATC 6 cut(s) 1270, 1673, 1923, 1945, 2491, 3245
BpiI GAAGAC 2 cut(s) 227, 2544
BpmI CTGGAG 3 cut(s) 476, 2597, 2843
Bpu1102I GCTNAGC 1 cut(s) 2796
BpuEI CTTGAG 1 cut(s) 2208
BsaAI YACGTR 1 cut(s) 2420
BsaBI GATNNNNATC 1 cut(s) 333
BsaHI GRCGYC 1 cut(s) 2746
BsaI GGTCTC 1 cut(s) 1787
BsaJI CCNNGG 5 cut(s) 428, 1827, 2035, 2314, 3229
BsaWI WCCGGW 1 cut(s) 975
BsaXI ACNNNNNCTCC 2 cut(s) 919, 949
Bsc4I CCNNNNNNNGG 6 cut(s) 365, 863, 982, 1136, 1828, 2511
Bse118I RCCGGY 2 cut(s) 863, 3034
Bse1I ACTGG 4 cut(s) 493, 991, 1924, 2281
Bse21I CCTNAGG 1 cut(s) 1185
Bse3DI GCAATG 4 cut(s) 375, 727, 1457, 3089
Bse8I GATNNNNATC 1 cut(s) 333
BseAI TCCGGA 1 cut(s) 975
BseBI CCWGG 5 cut(s) 282, 858, 1518, 2036, 2861
BseDI CCNNGG 5 cut(s) 428, 1827, 2035, 2314, 3229
BseGI GGATG 7 cut(s) 12, 91, 277, 1936, 1938, 1940, 2747
BseJI GATNNNNATC 1 cut(s) 333
BseLI CCNNNNNNNGG 6 cut(s) 365, 863, 982, 1136, 1828, 2511
BseMI GCAATG 4 cut(s) 375, 727, 1457, 3089
BseMII CTCAG 4 cut(s) 639, 1176, 2263, 2810
BseNI ACTGG 4 cut(s) 493, 991, 1924, 2281
BseRI GAGGAG 3 cut(s) 1268, 1319, 3086
BseSI GKGCMC 1 cut(s) 2829
BseXI GCAGC 7 cut(s) 60, 772, 977, 1720, 1859, 2554, 2972
BseYI CCCAGC 1 cut(s) 1894
BsgI GTGCAG 3 cut(s) 140, 771, 937
Bsh1236I CGCG 1 cut(s) 2717
BshFI GGCC 3 cut(s) 424, 745, 3034
BshNI GGYRCC 2 cut(s) 860, 2745
BsiHKAI GWGCWC 1 cut(s) 937
BsiHKCI CYCGRG 2 cut(s) 131, 998
BsiSI CCGG 3 cut(s) 864, 976, 3035
BslFI GGGAC 3 cut(s) 1112, 1911, 2800
BslI CCNNNNNNNGG 6 cut(s) 365, 863, 982, 1136, 1828, 2511
BsmAI GTCTC 5 cut(s) 128, 554, 1787, 2143, 3140
BsmFI GGGAC 3 cut(s) 1112, 1911, 2800
BsmI GAATGC 3 cut(s) 1243, 2455, 2872
BsnI GGCC 3 cut(s) 424, 745, 3034
Bso31I GGTCTC 1 cut(s) 1787
BsoBI CYCGRG 2 cut(s) 131, 998
Bsp1286I GDGCHC 2 cut(s) 937, 2829
Bsp13I TCCGGA 1 cut(s) 975
Bsp1407I TGTACA 1 cut(s) 1559
Bsp143I GATC 7 cut(s) 126, 487, 1093, 1180, 2095, 2297, 3064
Bsp1720I GCTNAGC 1 cut(s) 2796
Bsp19I CCATGG 2 cut(s) 1827, 3229
BspACI CCGC 3 cut(s) 950, 1786, 2983
BspANI GGCC 3 cut(s) 424, 745, 3034
BspCNI CTCAG 4 cut(s) 638, 1177, 2262, 2809
BspEI TCCGGA 1 cut(s) 975
BspFNI CGCG 1 cut(s) 2717
BspHI TCATGA 1 cut(s) 3067
BspLI GGNNCC 6 cut(s) 128, 852, 862, 1128, 2312, 2747
BspMI ACCTGC 1 cut(s) 999
BspPI GGATC 3 cut(s) 121, 134, 1175
BspQI GCTCTTC 1 cut(s) 542
BspT107I GGYRCC 2 cut(s) 860, 2745
BspTNI GGTCTC 1 cut(s) 1787
BsrDI GCAATG 4 cut(s) 375, 727, 1457, 3089
BsrFI RCCGGY 2 cut(s) 863, 3034
BsrGI TGTACA 1 cut(s) 1559
BsrI ACTGG 4 cut(s) 493, 991, 1924, 2281
BssAI RCCGGY 2 cut(s) 863, 3034
BssECI CCNNGG 5 cut(s) 428, 1827, 2035, 2314, 3229
BssMI GATC 7 cut(s) 126, 487, 1093, 1180, 2095, 2297, 3064
BssNAI GTATAC 1 cut(s) 3274
BssNI GRCGYC 1 cut(s) 2746
BssT1I CCWWGG 4 cut(s) 428, 1827, 2314, 3229
Bst1107I GTATAC 1 cut(s) 3274
Bst2UI CCWGG 5 cut(s) 282, 858, 1518, 2036, 2861
Bst6I CTCTTC 3 cut(s) 5, 542, 624
BstACI GRCGYC 1 cut(s) 2746
BstAPI GCANNNNNTGC 2 cut(s) 1853, 2408
BstAUI TGTACA 1 cut(s) 1559
BstBAI YACGTR 1 cut(s) 2420
BstC8I GCNNGC 4 cut(s) 727, 1645, 1887, 2155
BstDEI CTNAG 5 cut(s) 625, 1185, 2249, 2796, 3213
BstDSI CCRYGG 2 cut(s) 1827, 3229
BstF5I GGATG 7 cut(s) 12, 91, 277, 1936, 1938, 1940, 2747
BstFNI CGCG 1 cut(s) 2717
BstH2I RGCGCY 1 cut(s) 2749
BstHHI GCGC 1 cut(s) 2748
BstKTI GATC 7 cut(s) 129, 490, 1096, 1183, 2098, 2300, 3067
BstMAI GTCTC 5 cut(s) 128, 554, 1787, 2143, 3140
BstMBI GATC 7 cut(s) 126, 487, 1093, 1180, 2095, 2297, 3064
BstMWI GCNNNNNNNGC 4 cut(s) 726, 1853, 1891, 2408
BstNI CCWGG 5 cut(s) 282, 858, 1518, 2036, 2861
BstNSI RCATGY 4 cut(s) 236, 544, 933, 1688
BstSCI CCNGG 5 cut(s) 280, 856, 1516, 2034, 2859
BstSFI CTRYAG 3 cut(s) 2133, 2847, 3057
BstSLI GKGCMC 1 cut(s) 2829
BstUI CGCG 1 cut(s) 2717
BstV1I GCAGC 7 cut(s) 60, 772, 977, 1720, 1859, 2554, 2972
BstV2I GAAGAC 2 cut(s) 227, 2544
BstX2I RGATCY 1 cut(s) 126
BstXI CCANNNNNNTGG 1 cut(s) 3030
BstYI RGATCY 1 cut(s) 126
BstZ17I GTATAC 1 cut(s) 3274
Bsu36I CCTNAGG 1 cut(s) 1185
BsuI GTATCC 1 cut(s) 3004
BsuRI GGCC 3 cut(s) 424, 745, 3034
BtgI CCRYGG 2 cut(s) 1827, 3229
BtsCI GGATG 7 cut(s) 12, 91, 277, 1936, 1938, 1940, 2747
BtsIMutI CAGTG 6 cut(s) 788, 2346, 2475, 2652, 2691, 2973
BveI ACCTGC 1 cut(s) 999
Cac8I GCNNGC 4 cut(s) 727, 1645, 1887, 2155
CaiI CAGNNNCTG 2 cut(s) 326, 1345
CciI TCATGA 1 cut(s) 3067
CfoI GCGC 1 cut(s) 2748
Cfr10I RCCGGY 2 cut(s) 863, 3034
Cfr13I GGNCC 4 cut(s) 344, 851, 1126, 1994
CseI GACGC 2 cut(s) 2442, 2927
Csp6I GTAC 7 cut(s) 861, 1361, 1560, 1659, 1927, 2047, 2711
CviQI GTAC 7 cut(s) 861, 1361, 1560, 1659, 1927, 2047, 2711
DdeI CTNAG 5 cut(s) 625, 1185, 2249, 2796, 3213
DinI GGCGCC 1 cut(s) 2747
DpnI GATC 7 cut(s) 128, 489, 1095, 1182, 2097, 2299, 3066
DpnII GATC 7 cut(s) 126, 487, 1093, 1180, 2095, 2297, 3064
DrdI GACNNNNNNGTC 1 cut(s) 142
DseDI GACNNNNNNGTC 1 cut(s) 142
EaeI YGGCCR 1 cut(s) 3032
Eam1104I CTCTTC 3 cut(s) 5, 542, 624
EarI CTCTTC 3 cut(s) 5, 542, 624
EciI GGCGGA 1 cut(s) 1775
Eco130I CCWWGG 4 cut(s) 428, 1827, 2314, 3229
Eco147I AGGCCT 1 cut(s) 424
Eco31I GGTCTC 1 cut(s) 1787
Eco32I GATATC 1 cut(s) 373
Eco47I GGWCC 4 cut(s) 344, 851, 1126, 1994
Eco57I CTGAAG 1 cut(s) 1367
Eco72I CACGTG 1 cut(s) 2420
Eco81I CCTNAGG 1 cut(s) 1185
Eco88I CYCGRG 2 cut(s) 131, 998
EcoO109I RGGNCCY 1 cut(s) 851
EcoRI GAATTC 2 cut(s) 829, 1589
EcoRII CCWGG 5 cut(s) 280, 856, 1516, 2034, 2859
EcoRV GATATC 1 cut(s) 373
EcoT14I CCWWGG 4 cut(s) 428, 1827, 2314, 3229
EcoT22I ATGCAT 4 cut(s) 407, 1618, 1938, 3002
EgeI GGCGCC 1 cut(s) 2747
EheI GGCGCC 1 cut(s) 2747
ErhI CCWWGG 4 cut(s) 428, 1827, 2314, 3229
FalI AAGNNNNNCTT 2 cut(s) 2633, 2665
FaqI GGGAC 3 cut(s) 1112, 1911, 2800
FauNDI CATATG 2 cut(s) 2636, 3139
FbaI TGATCA 3 cut(s) 1093, 2095, 3064
FblI GTMKAC 2 cut(s) 2456, 3273
Fnu4HI GCNGC 7 cut(s) 49, 786, 966, 1734, 1848, 2568, 2986
FokI GGATG 7 cut(s) 19, 98, 264, 1923, 1927, 1945, 2754
Fsp4HI GCNGC 7 cut(s) 49, 786, 966, 1734, 1848, 2568, 2986
FspBI CTAG 8 cut(s) 45, 53, 956, 1019, 1634, 2469, 2492, 2930
GlaI GCGC 1 cut(s) 2747
GluI GCNGC 7 cut(s) 49, 786, 966, 1734, 1848, 2568, 2986
GsaI CCCAGC 1 cut(s) 1898
GsuI CTGGAG 3 cut(s) 476, 2597, 2843
HaeII RGCGCY 1 cut(s) 2749
HaeIII GGCC 3 cut(s) 424, 745, 3034
HapII CCGG 3 cut(s) 864, 976, 3035
HgaI GACGC 2 cut(s) 2442, 2927
HhaI GCGC 1 cut(s) 2748
Hin1I GRCGYC 1 cut(s) 2746
Hin6I GCGC 1 cut(s) 2746
HinP1I GCGC 1 cut(s) 2746
HincII GTYRAC 1 cut(s) 988
HindII GTYRAC 1 cut(s) 988
HindIII AAGCTT 1 cut(s) 2729
HpaI GTTAAC 1 cut(s) 988
HpaII CCGG 3 cut(s) 864, 976, 3035
HphI GGTGA 5 cut(s) 296, 815, 1420, 2202, 2333
Hpy166II GTNNAC 4 cut(s) 988, 2457, 2971, 3274
Hpy8I GTNNAC 4 cut(s) 988, 2457, 2971, 3274
HpyAV CCTTC 9 cut(s) 186, 607, 1405, 1478, 1875, 2071, 2635, 3105, 3186
HpyCH4IV ACGT 2 cut(s) 2419, 2877
HpyF10VI GCNNNNNNNGC 4 cut(s) 726, 1853, 1891, 2408
HpyF3I CTNAG 5 cut(s) 625, 1185, 2249, 2796, 3213
HpySE526I ACGT 2 cut(s) 2419, 2877
Hsp92I GRCGYC 1 cut(s) 2746
HspAI GCGC 1 cut(s) 2746
KasI GGCGCC 1 cut(s) 2745
Kpn2I TCCGGA 1 cut(s) 975
KpnI GGTACC 1 cut(s) 864
Ksp22I TGATCA 3 cut(s) 1093, 2095, 3064
KspAI GTTAAC 1 cut(s) 988
Kzo9I GATC 7 cut(s) 126, 487, 1093, 1180, 2095, 2297, 3064
LguI GCTCTTC 1 cut(s) 542
LmnI GCTCC 3 cut(s) 940, 1342, 2578
Lsp1109I GCAGC 7 cut(s) 60, 772, 977, 1720, 1859, 2554, 2972
LweI GCATC 6 cut(s) 1270, 1673, 1923, 1945, 2491, 3245
MaeI CTAG 8 cut(s) 45, 53, 956, 1019, 1634, 2469, 2492, 2930
MaeII ACGT 2 cut(s) 2419, 2877
MalI GATC 7 cut(s) 128, 489, 1095, 1182, 2097, 2299, 3066
MboI GATC 7 cut(s) 126, 487, 1093, 1180, 2095, 2297, 3064
MflI RGATCY 1 cut(s) 126
MhlI GDGCHC 2 cut(s) 937, 2829
Mly113I GGCGCC 1 cut(s) 2746
MlyI GAGTC 4 cut(s) 217, 805, 966, 2606
MmeI TCCRAC 1 cut(s) 346
Mph1103I ATGCAT 4 cut(s) 407, 1618, 1938, 3002
MroI TCCGGA 1 cut(s) 975
MroXI GAANNNNTTC 2 cut(s) 167, 477
MslI CAYNNNNRTG 1 cut(s) 282
MspA1I CMGCKG 4 cut(s) 272, 2567, 2800, 2985
MspI CCGG 3 cut(s) 864, 976, 3035
MspR9I CCNGG 5 cut(s) 282, 858, 1518, 2036, 2861
Mva1269I GAATGC 3 cut(s) 1243, 2455, 2872
MvaI CCWGG 5 cut(s) 282, 858, 1518, 2036, 2861
MvnI CGCG 1 cut(s) 2717
MwoI GCNNNNNNNGC 4 cut(s) 726, 1853, 1891, 2408
NarI GGCGCC 1 cut(s) 2746
NcoI CCATGG 2 cut(s) 1827, 3229
NdeI CATATG 2 cut(s) 2636, 3139
NdeII GATC 7 cut(s) 126, 487, 1093, 1180, 2095, 2297, 3064
NlaIV GGNNCC 6 cut(s) 128, 852, 862, 1128, 2312, 2747
NmuCI GTSAC 7 cut(s) 228, 284, 886, 993, 1079, 2471, 2990
NsiI ATGCAT 4 cut(s) 407, 1618, 1938, 3002
NspI RCATGY 4 cut(s) 236, 544, 933, 1688
PaeR7I CTCGAG 1 cut(s) 998
PagI TCATGA 1 cut(s) 3067
PceI AGGCCT 1 cut(s) 424
PciI ACATGT 1 cut(s) 929
PciSI GCTCTTC 1 cut(s) 542
PctI GAATGC 3 cut(s) 1243, 2455, 2872
PdmI GAANNNNTTC 2 cut(s) 167, 477
PflFI GACNNNGTC 2 cut(s) 226, 1418
PflMI CCANNNNNTGG 1 cut(s) 365
PfoI TCCNGGA 2 cut(s) 1516, 2859
PkrI GCNGC 7 cut(s) 50, 787, 967, 1735, 1849, 2569, 2987
PleI GAGTC 4 cut(s) 217, 805, 966, 2606
PluTI GGCGCC 1 cut(s) 2749
PmaCI CACGTG 1 cut(s) 2420
PmlI CACGTG 1 cut(s) 2420
PpsI GAGTC 4 cut(s) 217, 805, 966, 2606
Ppu21I YACGTR 1 cut(s) 2420
PpuMI RGGWCCY 1 cut(s) 851
PscI ACATGT 1 cut(s) 929
PshBI ATTAAT 4 cut(s) 408, 842, 1605, 1619
PsiI TTATAA 2 cut(s) 2163, 2526
Psp5II RGGWCCY 1 cut(s) 851
Psp6I CCWGG 5 cut(s) 280, 856, 1516, 2034, 2859
PspCI CACGTG 1 cut(s) 2420
PspFI CCCAGC 1 cut(s) 1894
PspGI CCWGG 5 cut(s) 280, 856, 1516, 2034, 2859
PspN4I GGNNCC 6 cut(s) 128, 852, 862, 1128, 2312, 2747
PspPI GGNCC 4 cut(s) 344, 851, 1126, 1994
PspPPI RGGWCCY 1 cut(s) 851
PspXI VCTCGAGB 1 cut(s) 998
PsrI GAACNNNNNNTAC 2 cut(s) 1543, 1575
PstNI CAGNNNCTG 2 cut(s) 326, 1345
PsuI RGATCY 1 cut(s) 126
PsyI GACNNNGTC 2 cut(s) 226, 1418
PvuII CAGCTG 3 cut(s) 272, 2567, 2800
RsaI GTAC 7 cut(s) 862, 1362, 1561, 1660, 1928, 2048, 2712
RsaNI GTAC 7 cut(s) 861, 1361, 1560, 1659, 1927, 2047, 2711
RseI CAYNNNNRTG 1 cut(s) 282
SapI GCTCTTC 1 cut(s) 542
SatI GCNGC 7 cut(s) 49, 786, 966, 1734, 1848, 2568, 2986
Sau3AI GATC 7 cut(s) 126, 487, 1093, 1180, 2095, 2297, 3064
Sau96I GGNCC 4 cut(s) 344, 851, 1126, 1994
ScaI AGTACT 1 cut(s) 2712
SchI GAGTC 4 cut(s) 217, 805, 966, 2606
ScrFI CCNGG 5 cut(s) 282, 858, 1518, 2036, 2861
SduI GDGCHC 2 cut(s) 937, 2829
SfaNI GCATC 6 cut(s) 1270, 1673, 1923, 1945, 2491, 3245
SfcI CTRYAG 3 cut(s) 2133, 2847, 3057
SfoI GGCGCC 1 cut(s) 2747
Sfr274I CTCGAG 1 cut(s) 998
SinI GGWCC 4 cut(s) 344, 851, 1126, 1994
SlaI CTCGAG 1 cut(s) 998
SmiMI CAYNNNNRTG 1 cut(s) 282
SmlI CTYRAG 2 cut(s) 998, 2223
SmoI CTYRAG 2 cut(s) 998, 2223
SseBI AGGCCT 1 cut(s) 424
SsiI CCGC 3 cut(s) 950, 1786, 2983
SspDI GGCGCC 1 cut(s) 2745
SspI AATATT 3 cut(s) 946, 1196, 1653
SspMI CTAG 8 cut(s) 45, 53, 956, 1019, 1634, 2469, 2492, 2930
StuI AGGCCT 1 cut(s) 424
StyD4I CCNGG 5 cut(s) 280, 856, 1516, 2034, 2859
StyI CCWWGG 4 cut(s) 428, 1827, 2314, 3229
TaiI ACGT 2 cut(s) 2422, 2880
TaqI TCGA 8 cut(s) 512, 999, 1264, 2387, 2610, 2735, 3118, 3179
TaqII GACCGA 1 cut(s) 361
TatI WGTACW 4 cut(s) 1559, 1658, 1926, 2710
TscAI CASTG 6 cut(s) 795, 2353, 2482, 2659, 2698, 2973
TseFI GTSAC 7 cut(s) 228, 284, 886, 993, 1079, 2471, 2990
TseI GCWGC 7 cut(s) 48, 785, 965, 1733, 1847, 2567, 2985
Tsp45I GTSAC 7 cut(s) 228, 284, 886, 993, 1079, 2471, 2990
TspGWI ACGGA 4 cut(s) 593, 2275, 2527, 3236
TspRI CASTG 6 cut(s) 795, 2353, 2482, 2659, 2698, 2973
Tth111I GACNNNGTC 2 cut(s) 226, 1418
Van91I CCANNNNNTGG 1 cut(s) 365
VpaK11BI GGWCC 4 cut(s) 344, 851, 1126, 1994
VspI ATTAAT 4 cut(s) 408, 842, 1605, 1619
XapI RAATTY 8 cut(s) 249, 476, 829, 1322, 1589, 2090, 2770, 2959
XceI RCATGY 4 cut(s) 236, 544, 933, 1688
XcmI CCANNNNNNNNNTGG 1 cut(s) 1988
XhoI CTCGAG 1 cut(s) 998
XmiI GTMKAC 2 cut(s) 2456, 3273
XmnI GAANNNNTTC 2 cut(s) 167, 477
XspI CTAG 8 cut(s) 45, 53, 956, 1019, 1634, 2469, 2492, 2930
ZrmI AGTACT 1 cut(s) 2712
Zsp2I ATGCAT 4 cut(s) 407, 1618, 1938, 3002
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.