RchiOBHm_Chr3g0488011

AAA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000279
Physical Location & Seq
Forward (+)
35514061 .. 35520654
6594 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45242

Sequence Viewer

Length: 3585 bp
ATGGGGATGAAGAGTAAGCTGAAACAGGAACAAGTTACAGATACTAGCAGCCTAGTGGGTTTGGTATTCTCTTGGTCTATTTCGGATGTTCTCAATGAAAATCTTTACAGAAATCAGGTGCAGAGGATCCCCGACACATTTGTGACATTGACGAGTTACAAGAAATCATTCATTCCTTCACTTGTTGAGGAAACTCATGCTGATTTACTATCGAATATGAAGACTCTGTCACGTGCACCAACTTGTGAAATTCTGACAATCGAAGATTCAGCTGGACCTCCTGGTGTCTTGTTTTACGATATAACGTATAACAGAGATACAGAAACTGATAAGAATCGCAAAGGACCGATGTATGAGCCACAGGTTGGAGATATCATTGCCTTGACAAATGTTAGACCAAAATGCATTGATGATTTGAACAGGCCTCCAAGGTTCTATCTAATCGCTTATGTTGATAAAGCAAATGATATTGATGAATTTCCTGATGATCTCCGGATTCTCCCGTTTAAAATACTATCATCGAAGCCTATCAACTATGGAGAACCAGACATGCATAAGAGCAAGAGAGAAACACTTTTTGCTGTCTATCTTTTGAACTTGACAACAAATCTCCGTGTATGGAAGGCTTTGAACTCAGAAGAGGGAAATACGAATATCATTAGCAAAGTTCTGCAACCCAATTCAGATGATGGGGATTCTTGTTCAGTTTGCTTCTCACAAGAAAAATGCAATACTGGCATTTCTGCTGTACGGCCTACAATTTTTTCTCAAAATCTAAATGAATCCCAAGAAGCTGCTGTTTTGAACTGTATCAGTTTGACTCAATGCCATCACCAGAATTCTGTAAAATTAATATGGGGTCCTCCTGGTACTGGCAAGACAAAGACAATGAGTGTGACACTCTTTGCTCTCTTTCAGTTAAAGTGCAGAACTCTCACATGCGCTCCCACCAATATTGCGGTGCTAGAAGTAGCAGCAAGACTCCGGAGATTGGTTAACCAATCACTCGAGTATCGCAGGTATGGACTTGGAGATATAGTTCTCTTTGGTAACAAGAAGCGAATGAAGATTGACAACAATAATGATCTTCGTGACATGTTTCTTGATCATCGTGTTAAAATCCTCATCAAGTGTTTGGTCCCTTTATCTGGGTGGAGACATTTGTTAGAATCAATGATACATTTACTTGATGATCCTGAGGAACAATATTCTTTGTATTTGGAACAGAGAGCTGAAAAGCAGAAACAGAATGCACAAAAAAATGAGGTGGATAATCGAAAACGTAATGGAGATGAAGATTATCCGTTCACATTTGAGGAGTTTGTGAAGAATGAATTTGATTCAATAGGTCAGGAGCTGAAGAAATGTATGGTAAATTTGTACACCCACTTACCAACTTCTTGTATTTCACTCGAAGTGGTGAAGGACATGGTCAGAGCTTTGGGTTTGCTCAACTCTATTGAATCTTCATTGCATACTATTGGTGTTGCTAATGAAGGGTTGAAGTTAATCAAAGATTTCAAAGTTCCAGGAAGCGTTGTTGGTTGCCTTATGCAGTTGAGAACAGAGTGTACAAACACACTAAAGTCGCTTCCAATGGAATTCTCTGTTCCTATTAACAAATATGCATTAGAGAACTTCTGCCTAGAAAATGCTTGCTTAATATTCTGTACTGCATCAACTTCCTCCAAATTGCATGTTGTAAAAGGGACAAGACCACTGGAGTTGTTGGTCATTGATGAAGCTGCTCAGCTTAAAGAATGTGAATCAGCAATTCCTTTACAACTATCTGGTCTCCGCCATGCTATCCTTGTAGGAGATGAGAGGCAACTCCCTGCCATGGTTGAAAGCAAGATTGCAGCAAGTGCTGATTTTGGAAGAAGTTTGTTTGGAAGGCTGGCAAAGCTGGGACACAAGAAGCACCTACTCAATGTCCAGTACAGGATGCATCCATCCATCAGTTTATTTCCGAAAAGGGAGTTCTACGACAACCAGATAGTAGATGGTCCAAATGTCAAAGAAAGAAGCTATGAGAGGTGCTTCCTCATGGGAAAAATGTACCAATCCTTTTCCTTCATAAATGTAGCCAATGGAAAAGAAGAATTTGATCACAGATTTAGTCAGAAAAATATGGTGGAGGTTTCTGTAGTCTCTGGGATAGTAGCAAGCCTTTATAAAGAATTTATTGGAACAAAGAAGAAAGTTAGTATTGGGGTCATATCACCATACAAGGCTCAAGTTCATGCAATTCAAGAGATACTCAGAAAATATACTGAAACTTCTGATACTGGATTCTCCGTAAGTGTCCGATCTGTTGATGGGTTCCAAGGTGGTGAAGAAGATGTGATAATTATATCTACTGTCCGATGTAATGGGAATGGGTCAGTTGGTTTCTTGTCGAACCGGCAAAGAGCAAATGTTGCCTTAACACGTGCAAGGTATTGCCTGTGGATATTGGGGAATGCGTCAACTTTGATTGCTAGTGACACTGTTTGGAAGAAGCTAGTCCTTGATGCCAAGAAACGGAACTGTTTTTATAATGCTGATGAAGACAGCAAGTTGACTCAGGCTATTACAGCTGCCCTGCTGGAGCTTGACCAACTTCATACTCTGCTTAATATCGACTCTATGCTGTTCAAAAACGCCATATGGAAGGTTTGCTTCACTCGTGACTTTTTGAACTCCATAACAAAAATAAAAGACACTGCGATTCTTTGGGATGTGCTGGCTTTATTAACCAAGCTTTCGAGTGGATGGCGCCGACCTCTCAAGGATAAAGGAACTTTAGTGCATGATAGGACTTCTGCTCAACTGTTAGAGAATTATAAAATCAAAGGGAACTTGAATCTCATTTGGACTGTAGATATTCTCAAGGAGAATGCACATTACATCCAAATTATGAAGTTTTTGGATATTTTGCCATTTTCTCATATACCAGAACTAGCAAAGCGTCTTGACATTGTTTTTGGGAATTTTACAGTGGACAAGATGAACCGCTGCCGTCACAAATGCATTGACAGGGATACTATCGTTCCAATGAGATGGCCGGTGGTCTTGGGCAATTTCCCTGTAGCTGATCATGAGGAGTTCCTTTCAAAACCATTGCCTTCTTTCAGTATAACAACTAATCGAGAAACAGCAACTTCAACATATGGGGAGACAGCGAAAGCAGTAAAACCAATTATCCTTTCGAGAAGCATTGTCAATCAAAAAGAAGGATTGTTATGGAAAATAAACGCTGAAGGAGGAGTGAAGGATCTCAGCACTTGCCTTGAAGCTAACACTTTGGAGTTCTTGTCAAAAAAATTATCATCACTAATTCTAGCAGCTAAGCCAGAAGCATCTAATTCCACTGACATGGAGACAGCGAAAGCAATAAAACCAATTGTCCCTTCGAAAAGCAATGTCAGCCAAAAAGAAAGATTGTTATGGAAAATAAAGGGTGAAGAAGGAGTGAAGGATTTCAGCACTTGCCTTGAAGCTATACTTTTGGAGTTCTTGTCAAAACCATTATCATCACTAATGGTTTTGGGGCTGCTTCTGCTTTTGGGATTGCTTTTGGGCTTACAGTATGGTGTTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12

Protein Analysis

1194

Amino Acids

134.59

Weight (kDa)

8.63

Isoelectric Point (pI)

36.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 80 - 212 7.6e-14 Domain of unknown function (DUF6469)
AAA_11 PF13086 258 - 426 1.4e-14 AAA domain
AAA_11 PF13086 543 - 618 1.9e-15 AAA domain
AAA_12 PF13087 626 - 822 5.1e-57 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000279)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65810 AT1G65810 AT1G65810 AT4G05540 AT5G37140 AT5G37150 AT5G37160 AT5G37165 AT5G52090
fragaria_vesca FvH4_1g27541 FvH4_1g27542 FvH4_2g08960 FvH4_2g08960 FvH4_2g08960 FvH4_2g08960 FvH4_3g00170 FvH4_3g00170 FvH4_3g00170 FvH4_5g05930 FvH4_5g05930
malus_domestica MD08G1077700.v1.1 MD08G1077900.v1.1 MD08G1078000.v1.1 MD08G1078100.v1.1 MD08G1078300.v1.1 MD09G1057800.v1.1 MD09G1281400.v1.1 MD11G1222900.v1.1 MD14G1146400.v1.1
prunus_persica Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G202100_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.3G013100_v2.0.a1
pyrus_communis pycom09g18900 pycom09g18910 pycom13g26720 pycom13g26730 pycom13g26760 pycom13g26770
rosa_chinensis RchiOBHm_Chr3g0488001 RchiOBHm_Chr3g0488011 RchiOBHm_Chr3g0488031 RchiOBHm_Chr3g0488061 RchiOBHm_Chr3g0488111 RchiOBHm_Chr3g0488201 RchiOBHm_Chr3g0488211 RchiOBHm_Chr3g0488221 RchiOBHm_Chr3g0488321 RchiOBHm_Chr3g0488431 RchiOBHm_Chr6g0264601 RchiOBHm_Chr6g0264641
rosa_laevigata RLG00000014240 RLG00000014241 RLG00000022910 RLG00000022949 RLG00000030376
rosa_multiflora Rmu_co8075388.1_g000001 Rmu_co8424869.1_g000001 Rmu_co8427783.1_g000001 Rmu_sc0000546.1_g000002 Rmu_sc0000546.1_g000015 Rmu_sc0011052.1_g000014 Rmu_sc0017772.1_g000004
rosa_roxburghii Rroxscaffold_175G00431980 Rroxscaffold_175G00431990 Rroxscaffold_1G00034600 Rroxscaffold_6G00394230 Rroxscaffold_6G00394270 Rroxscaffold_6G00394280 Rroxscaffold_6G00394350 Rroxscaffold_7G00203240 Rroxscaffold_7G00203270 Rroxscaffold_7G00203280 Rroxscaffold_7G00203300 Rroxscaffold_7G00205740
rosa_rugosa Rorug03G0238000 Rorug03G0238100 Rorug03G0238100 Rorug03G0238500 Rorug05G0155400 Rorug05G0356000 Rorug05G0463000 Rorug06G0015200 Rorug06G0015300 Rorug06G0015400 Rorug06G0015500 Rorug06G0015500
rosa_samantha Rh1DG088100 Rh2AG244800 Rh2BG187500 Rh2CG247300 Rh3BG322700 Rh3BG322800 Rh3BG323100 Rh3BG323900 Rh3BG324000 Rh3BG324300 Rh3CG321300 Rh3CG321400 Rh3CG321500 Rh3DG319400 Rh3DG319900 Rh3DG320100 Rh3DG320200 Rh6AG133700 Rh6AG133800 Rh6AG133900 Rh6CG129300 Rh6CG129800 Rh6CG130200 Rh6DG116500 Rh6DG117200 Rh7DG378000
rosa_wichuraiana Rw2G014710 Rw3G025340 Rw3G025350 Rw3G025360 Rw5G020630 Rw6G011590 Rw6G011600 Rw7G041380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2175, 2538, 2826
Acc36I ACCTGC 1 cut(s) 1008
AccB1I GGYRCC 1 cut(s) 2757
AccB7I CCANNNNNTGG 1 cut(s) 365
AccIII TCCGGA 2 cut(s) 492, 984
AciI CCGC 3 cut(s) 959, 1798, 2995
AclWI GGATC 4 cut(s) 121, 134, 1187, 3264
AcoI YGGCCR 1 cut(s) 3044
AcsI RAATTY 9 cut(s) 249, 476, 838, 1334, 1375, 1601, 2102, 2180, 2971
AcuI CTGAAG 2 cut(s) 1379, 3261
AcvI CACGTG 2 cut(s) 233, 2432
AcyI GRCGYC 1 cut(s) 2758
AfaI GTAC 7 cut(s) 750, 871, 1382, 1573, 1672, 1940, 2060
AfiI CCNNNNNNNGG 6 cut(s) 365, 872, 991, 1148, 1840, 2523
AflIII ACRYGT 2 cut(s) 1095, 2429
AjnI CCWGG 3 cut(s) 280, 865, 1528
AjuI GAANNNNNNNTTGG 4 cut(s) 3172, 3204, 3376, 3408
AleI CACNNNNGTG 1 cut(s) 140
AloI GAACNNNNNNTCC 2 cut(s) 1023, 1055
Alw21I GWGCWC 1 cut(s) 238
Alw26I GTCTC 5 cut(s) 1150, 1799, 2155, 3152, 3356
Alw44I GTGCAC 1 cut(s) 234
AlwI GGATC 4 cut(s) 121, 134, 1187, 3264
AlwNI CAGNNNCTG 2 cut(s) 326, 1357
Ama87I CYCGRG 1 cut(s) 1007
Aor13HI TCCGGA 2 cut(s) 492, 984
AoxI GGCC 3 cut(s) 422, 752, 3044
ApaLI GTGCAC 1 cut(s) 234
ApeKI GCWGC 9 cut(s) 48, 794, 974, 1745, 1859, 2579, 2997, 3326, 3535
ApoI RAATTY 9 cut(s) 249, 476, 838, 1334, 1375, 1601, 2102, 2180, 2971
ArsI GACNNNNNNTTYG 2 cut(s) 2948, 2980
AseI ATTAAT 1 cut(s) 851
Asp700I GAANNNNTTC 2 cut(s) 167, 3461
AspLEI GCGC 2 cut(s) 944, 2760
AspS9I GGNCC 5 cut(s) 275, 344, 860, 1138, 2006
AsuHPI GGTGA 5 cut(s) 824, 1432, 2214, 2345, 3455
AsuII TTCGAA 1 cut(s) 3395
AvaI CYCGRG 1 cut(s) 1007
AvaII GGWCC 5 cut(s) 275, 344, 860, 1138, 2006
AxyI CCTNAGG 1 cut(s) 1197
BaeGI GKGCMC 1 cut(s) 238
BaeI ACNNNNGTAYC 4 cut(s) 995, 1028, 3015, 3048
BamHI GGATCC 1 cut(s) 126
BanI GGYRCC 1 cut(s) 2757
BauI CACGAG 1 cut(s) 2667
BbrPI CACGTG 2 cut(s) 233, 2432
BbsI GAAGAC 2 cut(s) 227, 2556
Bbv12I GWGCWC 1 cut(s) 238
BbvI GCAGC 9 cut(s) 60, 781, 986, 1732, 1871, 2566, 2984, 3338, 3522
BccI CCATC 8 cut(s) 683, 837, 1960, 1964, 1997, 2312, 2748, 3036
BceAI ACGGC 2 cut(s) 767, 2985
BciT130I CCWGG 3 cut(s) 282, 867, 1530
BciVI GTATCC 1 cut(s) 3016
BclI TGATCA 3 cut(s) 1105, 2107, 3076
BcoDI GTCTC 5 cut(s) 1150, 1799, 2155, 3152, 3356
BfaI CTAG 8 cut(s) 45, 53, 965, 1646, 2481, 2504, 2942, 3323
BfmI CTRYAG 3 cut(s) 2145, 2859, 3069
BfoI RGCGCY 1 cut(s) 2761
BfuAI ACCTGC 1 cut(s) 1008
BfuI GTATCC 1 cut(s) 3016
BisI GCNGC 9 cut(s) 49, 795, 975, 1746, 1860, 2580, 2998, 3327, 3536
BlpI GCTNAGC 2 cut(s) 1749, 3330
BlsI GCNGC 9 cut(s) 50, 796, 976, 1747, 1861, 2581, 2999, 3328, 3537
Bme1390I CCNGG 3 cut(s) 282, 867, 1530
Bme18I GGWCC 5 cut(s) 275, 344, 860, 1138, 2006
BmeT110I CYCGRG 1 cut(s) 1007
BmgT120I GGNCC 5 cut(s) 275, 344, 860, 1138, 2006
BmiI GGNNCC 5 cut(s) 128, 861, 1140, 2324, 2759
BmrFI CCNGG 3 cut(s) 282, 867, 1530
BmsI GCATC 5 cut(s) 1685, 1935, 1957, 2503, 3350
BpiI GAAGAC 2 cut(s) 227, 2556
BpmI CTGGAG 2 cut(s) 1742, 2609
Bpu1102I GCTNAGC 2 cut(s) 1749, 3330
Bpu14I TTCGAA 1 cut(s) 3395
BpuEI CTTGAG 3 cut(s) 2220, 2753, 2855
BsaAI YACGTR 2 cut(s) 233, 2432
BsaBI GATNNNNATC 1 cut(s) 333
BsaHI GRCGYC 1 cut(s) 2758
BsaI GGTCTC 1 cut(s) 1799
BsaJI CCNNGG 3 cut(s) 428, 1839, 2326
BsaWI WCCGGW 2 cut(s) 492, 984
BsaXI ACNNNNNCTCC 8 cut(s) 928, 958, 1023, 1053, 3281, 3311, 3485, 3515
Bsc4I CCNNNNNNNGG 6 cut(s) 365, 872, 991, 1148, 1840, 2523
Bse118I RCCGGY 2 cut(s) 2403, 3046
Bse1I ACTGG 5 cut(s) 739, 877, 1725, 1936, 2293
Bse21I CCTNAGG 1 cut(s) 1197
Bse3DI GCAATG 4 cut(s) 375, 1469, 3101, 3409
Bse8I GATNNNNATC 1 cut(s) 333
BseAI TCCGGA 2 cut(s) 492, 984
BseBI CCWGG 3 cut(s) 282, 867, 1530
BseDI CCNNGG 3 cut(s) 428, 1839, 2326
BseGI GGATG 8 cut(s) 12, 91, 1948, 1950, 1952, 2725, 2759, 2889
BseJI GATNNNNATC 1 cut(s) 333
BseLI CCNNNNNNNGG 6 cut(s) 365, 872, 991, 1148, 1840, 2523
BseMI GCAATG 4 cut(s) 375, 1469, 3101, 3409
BseMII CTCAG 6 cut(s) 648, 1188, 1763, 2275, 2579, 3274
BseNI ACTGG 5 cut(s) 739, 877, 1725, 1936, 2293
BseRI GAGGAG 3 cut(s) 1331, 3098, 3261
BseSI GKGCMC 1 cut(s) 238
BseXI GCAGC 9 cut(s) 60, 781, 986, 1732, 1871, 2566, 2984, 3338, 3522
BseYI CCCAGC 1 cut(s) 1906
BsgI GTGCAG 2 cut(s) 140, 946
BshFI GGCC 3 cut(s) 424, 754, 3046
BshNI GGYRCC 1 cut(s) 2757
BsiHKAI GWGCWC 1 cut(s) 238
BsiHKCI CYCGRG 1 cut(s) 1007
BsiSI CCGG 4 cut(s) 493, 985, 2404, 3047
BslFI GGGAC 4 cut(s) 1124, 1723, 1923, 3374
BslI CCNNNNNNNGG 6 cut(s) 365, 872, 991, 1148, 1840, 2523
BsmAI GTCTC 5 cut(s) 1150, 1799, 2155, 3152, 3356
BsmFI GGGAC 4 cut(s) 1124, 1723, 1923, 3374
BsmI GAATGC 3 cut(s) 1255, 2467, 2884
BsnI GGCC 3 cut(s) 424, 754, 3046
Bso31I GGTCTC 1 cut(s) 1799
BsoBI CYCGRG 1 cut(s) 1007
Bsp119I TTCGAA 1 cut(s) 3395
Bsp1286I GDGCHC 1 cut(s) 238
Bsp13I TCCGGA 2 cut(s) 492, 984
Bsp1407I TGTACA 2 cut(s) 1380, 1571
Bsp143I GATC 9 cut(s) 126, 487, 1084, 1105, 1192, 2107, 2309, 3076, 3256
Bsp1720I GCTNAGC 2 cut(s) 1749, 3330
Bsp19I CCATGG 1 cut(s) 1839
BspACI CCGC 3 cut(s) 959, 1798, 2995
BspANI GGCC 3 cut(s) 424, 754, 3046
BspCNI CTCAG 6 cut(s) 647, 1189, 1762, 2274, 2578, 3273
BspEI TCCGGA 2 cut(s) 492, 984
BspHI TCATGA 1 cut(s) 3079
BspLI GGNNCC 5 cut(s) 128, 861, 1140, 2324, 2759
BspMI ACCTGC 1 cut(s) 1008
BspPI GGATC 4 cut(s) 121, 134, 1187, 3264
BspT104I TTCGAA 1 cut(s) 3395
BspT107I GGYRCC 1 cut(s) 2757
BspTNI GGTCTC 1 cut(s) 1799
BsrDI GCAATG 4 cut(s) 375, 1469, 3101, 3409
BsrFI RCCGGY 2 cut(s) 2403, 3046
BsrGI TGTACA 2 cut(s) 1380, 1571
BsrI ACTGG 5 cut(s) 739, 877, 1725, 1936, 2293
BssAI RCCGGY 2 cut(s) 2403, 3046
BssECI CCNNGG 3 cut(s) 428, 1839, 2326
BssMI GATC 9 cut(s) 126, 487, 1084, 1105, 1192, 2107, 2309, 3076, 3256
BssNI GRCGYC 1 cut(s) 2758
BssSI CACGAG 1 cut(s) 2667
BssT1I CCWWGG 3 cut(s) 428, 1839, 2326
Bst2BI CACGAG 1 cut(s) 2667
Bst2UI CCWGG 3 cut(s) 282, 867, 1530
Bst4CI ACNGT 8 cut(s) 809, 2362, 2491, 2531, 2814, 2860, 2980, 3570
Bst6I CTCTTC 2 cut(s) 5, 633
BstACI GRCGYC 1 cut(s) 2758
BstAPI GCANNNNNTGC 2 cut(s) 1865, 2420
BstAUI TGTACA 2 cut(s) 1380, 1571
BstBAI YACGTR 2 cut(s) 233, 2432
BstBI TTCGAA 1 cut(s) 3395
BstC8I GCNNGC 4 cut(s) 1657, 1899, 2167, 2727
BstDEI CTNAG 7 cut(s) 634, 1197, 1749, 2261, 2565, 3260, 3330
BstDSI CCRYGG 1 cut(s) 1839
BstF5I GGATG 8 cut(s) 12, 91, 1948, 1950, 1952, 2725, 2759, 2889
BstH2I RGCGCY 1 cut(s) 2761
BstHHI GCGC 2 cut(s) 944, 2760
BstKTI GATC 9 cut(s) 129, 490, 1087, 1108, 1195, 2110, 2312, 3079, 3259
BstMAI GTCTC 5 cut(s) 1150, 1799, 2155, 3152, 3356
BstMBI GATC 9 cut(s) 126, 487, 1084, 1105, 1192, 2107, 2309, 3076, 3256
BstMWI GCNNNNNNNGC 7 cut(s) 735, 1865, 1903, 2420, 2576, 3408, 3541
BstNI CCWGG 3 cut(s) 282, 867, 1530
BstNSI RCATGY 4 cut(s) 553, 942, 1099, 1700
BstSCI CCNGG 3 cut(s) 280, 865, 1528
BstSFI CTRYAG 3 cut(s) 2145, 2859, 3069
BstSLI GKGCMC 1 cut(s) 238
BstV1I GCAGC 9 cut(s) 60, 781, 986, 1732, 1871, 2566, 2984, 3338, 3522
BstV2I GAAGAC 2 cut(s) 227, 2556
BstX2I RGATCY 2 cut(s) 126, 3256
BstXI CCANNNNNNTGG 2 cut(s) 3042, 3358
BstYI RGATCY 2 cut(s) 126, 3256
Bsu36I CCTNAGG 1 cut(s) 1197
BsuI GTATCC 1 cut(s) 3016
BsuRI GGCC 3 cut(s) 424, 754, 3046
BtgI CCRYGG 1 cut(s) 1839
BtsCI GGATG 8 cut(s) 12, 91, 1948, 1950, 1952, 2725, 2759, 2889
BtsI GCAGTG 1 cut(s) 2703
BtsIMutI CAGTG 5 cut(s) 1718, 2487, 2703, 2985, 3351
BveI ACCTGC 1 cut(s) 1008
Cac8I GCNNGC 4 cut(s) 1657, 1899, 2167, 2727
CaiI CAGNNNCTG 2 cut(s) 326, 1357
CciI TCATGA 1 cut(s) 3079
CfoI GCGC 2 cut(s) 944, 2760
Cfr10I RCCGGY 2 cut(s) 2403, 3046
Cfr13I GGNCC 5 cut(s) 275, 344, 860, 1138, 2006
CseI GACGC 2 cut(s) 2454, 2939
Csp6I GTAC 7 cut(s) 749, 870, 1381, 1572, 1671, 1939, 2059
CspCI CAANNNNNGTGG 4 cut(s) 937, 972, 1707, 1742
CviQI GTAC 7 cut(s) 749, 870, 1381, 1572, 1671, 1939, 2059
DdeI CTNAG 7 cut(s) 634, 1197, 1749, 2261, 2565, 3260, 3330
DinI GGCGCC 1 cut(s) 2759
DpnI GATC 9 cut(s) 128, 489, 1086, 1107, 1194, 2109, 2311, 3078, 3258
DpnII GATC 9 cut(s) 126, 487, 1084, 1105, 1192, 2107, 2309, 3076, 3256
DraI TTTAAA 1 cut(s) 508
EaeI YGGCCR 1 cut(s) 3044
Eam1104I CTCTTC 2 cut(s) 5, 633
EarI CTCTTC 2 cut(s) 5, 633
EciI GGCGGA 1 cut(s) 1787
Eco130I CCWWGG 3 cut(s) 428, 1839, 2326
Eco147I AGGCCT 1 cut(s) 424
Eco31I GGTCTC 1 cut(s) 1799
Eco32I GATATC 1 cut(s) 373
Eco47I GGWCC 5 cut(s) 275, 344, 860, 1138, 2006
Eco57I CTGAAG 2 cut(s) 1379, 3261
Eco72I CACGTG 2 cut(s) 233, 2432
Eco81I CCTNAGG 1 cut(s) 1197
Eco88I CYCGRG 1 cut(s) 1007
EcoO109I RGGNCCY 1 cut(s) 860
EcoRI GAATTC 2 cut(s) 838, 1601
EcoRII CCWGG 3 cut(s) 280, 865, 1528
EcoRV GATATC 1 cut(s) 373
EcoT14I CCWWGG 3 cut(s) 428, 1839, 2326
EcoT22I ATGCAT 5 cut(s) 407, 555, 1630, 1950, 3014
EgeI GGCGCC 1 cut(s) 2759
EheI GGCGCC 1 cut(s) 2759
ErhI CCWWGG 3 cut(s) 428, 1839, 2326
FalI AAGNNNNNCTT 4 cut(s) 2645, 2677, 3471, 3503
FaqI GGGAC 4 cut(s) 1124, 1723, 1923, 3374
FauNDI CATATG 2 cut(s) 2648, 3151
FbaI TGATCA 3 cut(s) 1105, 2107, 3076
Fnu4HI GCNGC 9 cut(s) 49, 795, 975, 1746, 1860, 2580, 2998, 3327, 3536
FokI GGATG 8 cut(s) 19, 98, 1935, 1939, 1957, 2732, 2766, 2876
Fsp4HI GCNGC 9 cut(s) 49, 795, 975, 1746, 1860, 2580, 2998, 3327, 3536
FspBI CTAG 8 cut(s) 45, 53, 965, 1646, 2481, 2504, 2942, 3323
GlaI GCGC 2 cut(s) 943, 2759
GluI GCNGC 9 cut(s) 49, 795, 975, 1746, 1860, 2580, 2998, 3327, 3536
GsaI CCCAGC 1 cut(s) 1910
GsuI CTGGAG 2 cut(s) 1742, 2609
HaeII RGCGCY 1 cut(s) 2761
HaeIII GGCC 3 cut(s) 424, 754, 3046
HapII CCGG 4 cut(s) 493, 985, 2404, 3047
HgaI GACGC 2 cut(s) 2454, 2939
HhaI GCGC 2 cut(s) 944, 2760
Hin1I GRCGYC 1 cut(s) 2758
Hin6I GCGC 2 cut(s) 942, 2758
HinP1I GCGC 2 cut(s) 942, 2758
HincII GTYRAC 3 cut(s) 997, 2469, 2562
HindII GTYRAC 3 cut(s) 997, 2469, 2562
HindIII AAGCTT 1 cut(s) 2741
HpaI GTTAAC 1 cut(s) 997
HpaII CCGG 4 cut(s) 493, 985, 2404, 3047
HphI GGTGA 5 cut(s) 824, 1432, 2214, 2345, 3455
Hpy166II GTNNAC 8 cut(s) 236, 997, 1308, 1383, 1572, 2469, 2562, 2983
Hpy8I GTNNAC 8 cut(s) 236, 997, 1308, 1383, 1572, 2469, 2562, 2983
HpyCH4III ACNGT 8 cut(s) 809, 2362, 2491, 2531, 2814, 2860, 2980, 3570
HpyCH4IV ACGT 4 cut(s) 232, 305, 1282, 2431
HpyF10VI GCNNNNNNNGC 7 cut(s) 735, 1865, 1903, 2420, 2576, 3408, 3541
HpyF3I CTNAG 7 cut(s) 634, 1197, 1749, 2261, 2565, 3260, 3330
HpySE526I ACGT 4 cut(s) 232, 305, 1282, 2431
Hsp92I GRCGYC 1 cut(s) 2758
HspAI GCGC 2 cut(s) 942, 2758
KasI GGCGCC 1 cut(s) 2757
Kpn2I TCCGGA 2 cut(s) 492, 984
Ksp22I TGATCA 3 cut(s) 1105, 2107, 3076
KspAI GTTAAC 1 cut(s) 997
Kzo9I GATC 9 cut(s) 126, 487, 1084, 1105, 1192, 2107, 2309, 3076, 3256
LmnI GCTCC 3 cut(s) 949, 1354, 2590
Lsp1109I GCAGC 9 cut(s) 60, 781, 986, 1732, 1871, 2566, 2984, 3338, 3522
LweI GCATC 5 cut(s) 1685, 1935, 1957, 2503, 3350
MaeI CTAG 8 cut(s) 45, 53, 965, 1646, 2481, 2504, 2942, 3323
MaeII ACGT 4 cut(s) 232, 305, 1282, 2431
MalI GATC 9 cut(s) 128, 489, 1086, 1107, 1194, 2109, 2311, 3078, 3258
MboI GATC 9 cut(s) 126, 487, 1084, 1105, 1192, 2107, 2309, 3076, 3256
MfeI CAATTG 1 cut(s) 3384
MflI RGATCY 2 cut(s) 126, 3256
MhlI GDGCHC 1 cut(s) 238
Mly113I GGCGCC 1 cut(s) 2758
MlyI GAGTC 5 cut(s) 217, 814, 975, 2557, 2618
MmeI TCCRAC 1 cut(s) 346
Mph1103I ATGCAT 5 cut(s) 407, 555, 1630, 1950, 3014
MroI TCCGGA 2 cut(s) 492, 984
MroXI GAANNNNTTC 2 cut(s) 167, 3461
MslI CAYNNNNRTG 2 cut(s) 140, 3356
MspA1I CMGCKG 3 cut(s) 272, 2579, 2997
MspI CCGG 4 cut(s) 493, 985, 2404, 3047
MspR9I CCNGG 3 cut(s) 282, 867, 1530
MunI CAATTG 1 cut(s) 3384
Mva1269I GAATGC 3 cut(s) 1255, 2467, 2884
MvaI CCWGG 3 cut(s) 282, 867, 1530
MwoI GCNNNNNNNGC 7 cut(s) 735, 1865, 1903, 2420, 2576, 3408, 3541
NarI GGCGCC 1 cut(s) 2758
NcoI CCATGG 1 cut(s) 1839
NdeI CATATG 2 cut(s) 2648, 3151
NdeII GATC 9 cut(s) 126, 487, 1084, 1105, 1192, 2107, 2309, 3076, 3256
NlaIV GGNNCC 5 cut(s) 128, 861, 1140, 2324, 2759
NmuCI GTSAC 7 cut(s) 142, 228, 895, 1091, 2483, 2669, 3002
NsiI ATGCAT 5 cut(s) 407, 555, 1630, 1950, 3014
NspI RCATGY 4 cut(s) 553, 942, 1099, 1700
NspV TTCGAA 1 cut(s) 3395
OliI CACNNNNGTG 1 cut(s) 140
PaeR7I CTCGAG 1 cut(s) 1007
PagI TCATGA 1 cut(s) 3079
PceI AGGCCT 1 cut(s) 424
PciI ACATGT 1 cut(s) 1095
PctI GAATGC 3 cut(s) 1255, 2467, 2884
PdmI GAANNNNTTC 2 cut(s) 167, 3461
PflFI GACNNNGTC 2 cut(s) 226, 1430
PflMI CCANNNNNTGG 1 cut(s) 365
PfoI TCCNGGA 1 cut(s) 1528
PkrI GCNGC 9 cut(s) 50, 796, 976, 1747, 1861, 2581, 2999, 3328, 3537
PleI GAGTC 5 cut(s) 217, 814, 975, 2557, 2618
PluTI GGCGCC 1 cut(s) 2761
PmaCI CACGTG 2 cut(s) 233, 2432
PmlI CACGTG 2 cut(s) 233, 2432
PpsI GAGTC 5 cut(s) 217, 814, 975, 2557, 2618
Ppu21I YACGTR 2 cut(s) 233, 2432
PpuMI RGGWCCY 1 cut(s) 860
PscI ACATGT 1 cut(s) 1095
PshBI ATTAAT 1 cut(s) 851
PsiI TTATAA 3 cut(s) 2175, 2538, 2826
Psp5II RGGWCCY 1 cut(s) 860
Psp6I CCWGG 3 cut(s) 280, 865, 1528
PspCI CACGTG 2 cut(s) 233, 2432
PspFI CCCAGC 1 cut(s) 1906
PspGI CCWGG 3 cut(s) 280, 865, 1528
PspN4I GGNNCC 5 cut(s) 128, 861, 1140, 2324, 2759
PspPI GGNCC 5 cut(s) 275, 344, 860, 1138, 2006
PspPPI RGGWCCY 1 cut(s) 860
PspXI VCTCGAGB 1 cut(s) 1007
PsrI GAACNNNNNNTAC 2 cut(s) 1555, 1587
PstNI CAGNNNCTG 2 cut(s) 326, 1357
PsuI RGATCY 2 cut(s) 126, 3256
PsyI GACNNNGTC 2 cut(s) 226, 1430
PvuII CAGCTG 2 cut(s) 272, 2579
RsaI GTAC 7 cut(s) 750, 871, 1382, 1573, 1672, 1940, 2060
RsaNI GTAC 7 cut(s) 749, 870, 1381, 1572, 1671, 1939, 2059
RseI CAYNNNNRTG 2 cut(s) 140, 3356
SatI GCNGC 9 cut(s) 49, 795, 975, 1746, 1860, 2580, 2998, 3327, 3536
Sau3AI GATC 9 cut(s) 126, 487, 1084, 1105, 1192, 2107, 2309, 3076, 3256
Sau96I GGNCC 5 cut(s) 275, 344, 860, 1138, 2006
SchI GAGTC 5 cut(s) 217, 814, 975, 2557, 2618
ScrFI CCNGG 3 cut(s) 282, 867, 1530
SduI GDGCHC 1 cut(s) 238
SfaNI GCATC 5 cut(s) 1685, 1935, 1957, 2503, 3350
SfcI CTRYAG 3 cut(s) 2145, 2859, 3069
SfoI GGCGCC 1 cut(s) 2759
Sfr274I CTCGAG 1 cut(s) 1007
SfuI TTCGAA 1 cut(s) 3395
SinI GGWCC 5 cut(s) 275, 344, 860, 1138, 2006
SlaI CTCGAG 1 cut(s) 1007
SmiMI CAYNNNNRTG 2 cut(s) 140, 3356
SmlI CTYRAG 4 cut(s) 1007, 2235, 2768, 2870
SmoI CTYRAG 4 cut(s) 1007, 2235, 2768, 2870
SseBI AGGCCT 1 cut(s) 424
SsiI CCGC 3 cut(s) 959, 1798, 2995
SspDI GGCGCC 1 cut(s) 2757
SspI AATATT 3 cut(s) 955, 1208, 1665
SspMI CTAG 8 cut(s) 45, 53, 965, 1646, 2481, 2504, 2942, 3323
StuI AGGCCT 1 cut(s) 424
StyD4I CCNGG 3 cut(s) 280, 865, 1528
StyI CCWWGG 3 cut(s) 428, 1839, 2326
TaaI ACNGT 8 cut(s) 809, 2362, 2491, 2531, 2814, 2860, 2980, 3570
TaiI ACGT 4 cut(s) 235, 308, 1285, 2434
TaqII GACCGA 1 cut(s) 361
TatI WGTACW 4 cut(s) 1380, 1571, 1670, 1938
TscAI CASTG 5 cut(s) 1725, 2494, 2710, 2985, 3358
TseFI GTSAC 7 cut(s) 142, 228, 895, 1091, 2483, 2669, 3002
TseI GCWGC 9 cut(s) 48, 794, 974, 1745, 1859, 2579, 2997, 3326, 3535
Tsp45I GTSAC 7 cut(s) 142, 228, 895, 1091, 2483, 2669, 3002
TspGWI ACGGA 4 cut(s) 602, 1293, 2287, 2539
TspRI CASTG 5 cut(s) 1725, 2494, 2710, 2985, 3358
Tth111I GACNNNGTC 2 cut(s) 226, 1430
Van91I CCANNNNNTGG 1 cut(s) 365
VneI GTGCAC 1 cut(s) 234
VpaK11BI GGWCC 5 cut(s) 275, 344, 860, 1138, 2006
VspI ATTAAT 1 cut(s) 851
XapI RAATTY 9 cut(s) 249, 476, 838, 1334, 1375, 1601, 2102, 2180, 2971
XceI RCATGY 4 cut(s) 553, 942, 1099, 1700
XcmI CCANNNNNNNNNTGG 1 cut(s) 2000
XhoI CTCGAG 1 cut(s) 1007
XmnI GAANNNNTTC 2 cut(s) 167, 3461
XspI CTAG 8 cut(s) 45, 53, 965, 1646, 2481, 2504, 2942, 3323
Zsp2I ATGCAT 5 cut(s) 407, 555, 1630, 1950, 3014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.