FvH4_1g27542

AAA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000279
Physical Location & Seq
Reverse (-)
19193408 .. 19196007
2600 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g27542.t1

Sequence Viewer

Length: 1950 bp
ATGTCATTCACTCACTCACCTCGATTCTTTCCGACTGCCTCCGATCCGAGCTCGCCAAAAATCCCTACTCATGGTCGTCCTCGTCGTCTTCTCTCCAGTCAGTACTCATTCCGTTACCCTATGTTAGCTGGTTCAAGTGCACCGCGGTGCACTGAATCCGCTCCGATTGTGCAGAGGATCCCCGACACATTTGACACAGTGACGAGTTACAAGAAAACATTCATTCCTTCACTTGTTGAGGAAACTCATGCTGATTTACTATCGAATCTGTTGATTCTGCCACATGCATCTACTTGTGAAATTCTGACAATCATGGATTCCGATGAACCTCCTGATGAGTTGTTTTACGATGCTGGAGATATCATTGCCTTGACGAACGTTAGACCAAAATGCATTGATGATTTGAACAGGCCTCCAAGGTTCTATCTTATAGCTTATGTTGCTAAAGCAAATGATATTGATGAATATCCTGATCTCCTCCAGTTCAAAATACTGTCATCGAAGCCTATCAAATATGGAGAGGCAGGCATGAATAAGAGCAAGAGAGAAAAACTTTTTGCTGTCCGTCTTATAAACTTGATGACAAATGTCCGTGTATGGAAGGCTTTGAACTCAGAAGAGGGGAATACAAATATCATTAACAAAGTTCTGCAACCCAAGTCAGATGATGGGGATTCTTGTTCAGTCTGCTTCTCCAAAGAAAAATGCTGCACTAGAATTTCTGATATATGGCATACAATTCGCTCCCAAAATCTAGATGATTCCCAAGAAGCTGCAGTTTTGAACTGTATCAATTTGTCTCAATGTCATCACCAGAATTCTGTAAAATTAATATGGGGTCCTCCTGGTACTGGCAAGACAAAGACAATGAGTCTGACACTCTTTGCGCTCTTTCAGTTGAAGTGCAGAACTCTCACATGTGCTCCCACCAATATTGCCGTGCTAGAAATTGCAGCAAGGCTCCGAAGATCGGTTAATCAGTCACTTGAGTATGGCGGGTATGGACTAGGAGATATAGTTCTCTTTGGTAACAACAAGCGAATGAAGATTGACAATAACGATGATGTGCGTGACATATTTCTTGATCATCGTGCTAAAATCCTCATCAAGTGTTTGGTTCCTTTGTCTGGGTGGAAACATTTGTTATCATCAATGATACATTTACTTGATGATCCAGTGGAACATTATTCTATGTATTTGCAAAAGAGAGCTGCAAAGCAAAAACAGAATGCAGAAAAAAATGAGGAGGATGATCGAACTGAGGATTATCTTTTGACATTTGAGGAGTTTGTGAAGAATGAATTTGATTCAGTCAGTGAGGACCTGAAGATTTGTATGACAGATTTGTACACCCACTTACCAACTTCTTGCATTTCACTCGAAGTGGTAAAGGACATGGTAAGAGCTTTGGGTTTGCCCAAGCCTATTAAATCTTCATTGCATACTATTGGTGCTGCTAATGAAGGATTGAAGCTAATCCTCAATGACTTCAAAGTTCCCAGAAGCACTGTTGGTTGCCTTAGGCAGCTGAGAACAAAATGTACAAACACACTGAAGTCGCTTCCAAAGGAATTCTCTGTTCCTATTTCCGAATATGCATTAAAGAACTTCTGCCTAGAAAATGCTTGCTTAATATTCTGTACCGCATCAACTTCTTCCAAATTGCATATTGTAGCAGAAACAAGACCATTGGAATTGTTGGTCATTGACGAAGCTGCTCAGCTTAAAGAATGTGAATCAGCAATTCCTTTACAAATATCTGGTCTCCGCCATGCTATCCTTGTAGGAGATGAGAGGCAACTCCCTGGCATGGTTAAAAGCAAGATTGCAGCAAGTGCTGATTTTGGAAGAAGTTTGTTTGAAAGGCTGGCAAAGTTGGGACACAAGAAGCACCTGCTCAATGTCCAGTACAGGATGCATCCATCCATCAGTTTATTTCCAAAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12

Protein Analysis

650

Amino Acids

73.02

Weight (kDa)

8.52

Isoelectric Point (pI)

48.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 118 - 205 9.7e-10 Domain of unknown function (DUF6469)
AAA_11 PF13086 251 - 608 1.5e-32 AAA domain
AAA_12 PF13087 616 - 648 1.1e-07 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000279)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65810 AT1G65810 AT1G65810 AT4G05540 AT5G37140 AT5G37150 AT5G37160 AT5G37165 AT5G52090
fragaria_vesca FvH4_1g27541 FvH4_1g27542 FvH4_2g08960 FvH4_2g08960 FvH4_2g08960 FvH4_2g08960 FvH4_3g00170 FvH4_3g00170 FvH4_3g00170 FvH4_5g05930 FvH4_5g05930
malus_domestica MD08G1077700.v1.1 MD08G1077900.v1.1 MD08G1078000.v1.1 MD08G1078100.v1.1 MD08G1078300.v1.1 MD09G1057800.v1.1 MD09G1281400.v1.1 MD11G1222900.v1.1 MD14G1146400.v1.1
prunus_persica Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G202100_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.3G013100_v2.0.a1
pyrus_communis pycom09g18900 pycom09g18910 pycom13g26720 pycom13g26730 pycom13g26760 pycom13g26770
rosa_chinensis RchiOBHm_Chr3g0488001 RchiOBHm_Chr3g0488011 RchiOBHm_Chr3g0488031 RchiOBHm_Chr3g0488061 RchiOBHm_Chr3g0488111 RchiOBHm_Chr3g0488201 RchiOBHm_Chr3g0488211 RchiOBHm_Chr3g0488221 RchiOBHm_Chr3g0488321 RchiOBHm_Chr3g0488431 RchiOBHm_Chr6g0264601 RchiOBHm_Chr6g0264641
rosa_laevigata RLG00000014240 RLG00000014241 RLG00000022910 RLG00000022949 RLG00000030376
rosa_multiflora Rmu_co8075388.1_g000001 Rmu_co8424869.1_g000001 Rmu_co8427783.1_g000001 Rmu_sc0000546.1_g000002 Rmu_sc0000546.1_g000015 Rmu_sc0011052.1_g000014 Rmu_sc0017772.1_g000004
rosa_roxburghii Rroxscaffold_175G00431980 Rroxscaffold_175G00431990 Rroxscaffold_1G00034600 Rroxscaffold_6G00394230 Rroxscaffold_6G00394270 Rroxscaffold_6G00394280 Rroxscaffold_6G00394350 Rroxscaffold_7G00203240 Rroxscaffold_7G00203270 Rroxscaffold_7G00203280 Rroxscaffold_7G00203300 Rroxscaffold_7G00205740
rosa_rugosa Rorug03G0238000 Rorug03G0238100 Rorug03G0238100 Rorug03G0238500 Rorug05G0155400 Rorug05G0356000 Rorug05G0463000 Rorug06G0015200 Rorug06G0015300 Rorug06G0015400 Rorug06G0015500 Rorug06G0015500
rosa_samantha Rh1DG088100 Rh2AG244800 Rh2BG187500 Rh2CG247300 Rh3BG322700 Rh3BG322800 Rh3BG323100 Rh3BG323900 Rh3BG324000 Rh3BG324300 Rh3CG321300 Rh3CG321400 Rh3CG321500 Rh3DG319400 Rh3DG319900 Rh3DG320100 Rh3DG320200 Rh6AG133700 Rh6AG133800 Rh6AG133900 Rh6CG129300 Rh6CG129800 Rh6CG130200 Rh6DG116500 Rh6DG117200 Rh7DG378000
rosa_wichuraiana Rw2G014710 Rw3G025340 Rw3G025350 Rw3G025360 Rw5G020630 Rw6G011590 Rw6G011600 Rw7G041380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 572
AarI CACCTGC 1 cut(s) 1902
Acc36I ACCTGC 1 cut(s) 1902
AccBSI CCGCTC 1 cut(s) 161
AccII CGCG 1 cut(s) 145
AciI CCGC 6 cut(s) 143, 145, 159, 996, 1644, 1768
AclI AACGTT 1 cut(s) 378
AclWI GGATC 4 cut(s) 38, 172, 185, 1166
AcsI RAATTY 5 cut(s) 300, 717, 817, 1301, 1571
AcuI CTGAAG 2 cut(s) 1346, 1574
AfaI GTAC 6 cut(s) 104, 850, 1349, 1543, 1642, 1910
AfiI CCNNNNNNNGG 5 cut(s) 71, 851, 970, 1127, 1810
AflIII ACRYGT 1 cut(s) 917
AgsI TTSAA 9 cut(s) 135, 406, 487, 610, 784, 901, 1471, 1492, 1862
AhdI GACNNNNNGTC 1 cut(s) 870
AjnI CCWGG 2 cut(s) 844, 1804
AleI CACNNNNGTG 1 cut(s) 145
AloI GAACNNNNNNTCC 2 cut(s) 1002, 1034
Alw21I GWGCWC 4 cut(s) 53, 142, 152, 925
Alw26I GTCTC 2 cut(s) 804, 1769
Alw44I GTGCAC 2 cut(s) 138, 148
AlwI GGATC 4 cut(s) 38, 172, 185, 1166
AoxI GGCC 1 cut(s) 410
ApaLI GTGCAC 2 cut(s) 138, 148
ApeKI GCWGC 8 cut(s) 708, 773, 953, 1211, 1454, 1525, 1715, 1829
ApoI RAATTY 5 cut(s) 300, 717, 817, 1301, 1571
ArsI GACNNNNNNTTYG 2 cut(s) 1747, 1779
AseI ATTAAT 1 cut(s) 830
Asp700I GAANNNNTTC 1 cut(s) 218
AspLEI GCGC 1 cut(s) 889
AspS9I GGNCC 2 cut(s) 839, 1321
AsuHPI GGTGA 2 cut(s) 9, 803
AvaII GGWCC 2 cut(s) 839, 1321
AxyI CCTNAGG 1 cut(s) 1520
BaeGI GKGCMC 2 cut(s) 142, 152
BamHI GGATCC 1 cut(s) 177
BanII GRGCYC 1 cut(s) 53
BbsI GAAGAC 1 cut(s) 80
Bbv12I GWGCWC 4 cut(s) 53, 142, 152, 925
BbvI GCAGC 8 cut(s) 695, 760, 965, 1198, 1441, 1537, 1702, 1841
BccI CCATC 3 cut(s) 662, 1930, 1934
BceAI ACGGC 1 cut(s) 923
BcgI CGANNNNNNTGC 4 cut(s) 722, 756, 1360, 1394
BciT130I CCWGG 2 cut(s) 846, 1806
BclI TGATCA 1 cut(s) 1084
BcoDI GTCTC 2 cut(s) 804, 1769
BfaI CTAG 5 cut(s) 714, 755, 944, 1007, 1616
BfmI CTRYAG 1 cut(s) 774
BfuAI ACCTGC 1 cut(s) 1902
BisI GCNGC 8 cut(s) 709, 774, 954, 1212, 1455, 1526, 1716, 1830
BlpI GCTNAGC 1 cut(s) 1719
BlsI GCNGC 8 cut(s) 710, 775, 955, 1213, 1456, 1527, 1717, 1831
BmcAI AGTACT 1 cut(s) 104
Bme1390I CCNGG 2 cut(s) 846, 1806
Bme18I GGWCC 2 cut(s) 839, 1321
BmeRI GACNNNNNGTC 1 cut(s) 870
BmgT120I GGNCC 2 cut(s) 839, 1321
BmiI GGNNCC 4 cut(s) 179, 840, 962, 1119
BmrFI CCNGG 2 cut(s) 846, 1806
BmsI GCATC 5 cut(s) 296, 340, 1655, 1905, 1927
BoxI GACNNNNGTC 1 cut(s) 587
BpiI GAAGAC 1 cut(s) 80
BpmI CTGGAG 3 cut(s) 79, 375, 464
Bpu1102I GCTNAGC 1 cut(s) 1719
BpuEI CTTGAG 1 cut(s) 1007
BsaBI GATNNNNATC 1 cut(s) 465
BsaI GGTCTC 1 cut(s) 1769
BsaJI CCNNGG 3 cut(s) 143, 416, 1804
BsaXI ACNNNNNCTCC 4 cut(s) 907, 937, 1002, 1032
Bsc4I CCNNNNNNNGG 5 cut(s) 71, 851, 970, 1127, 1810
Bse1I ACTGG 5 cut(s) 96, 481, 856, 1175, 1906
Bse21I CCTNAGG 1 cut(s) 1520
Bse3DI GCAATG 2 cut(s) 363, 1436
Bse8I GATNNNNATC 1 cut(s) 465
BseBI CCWGG 2 cut(s) 846, 1806
BseDI CCNNGG 3 cut(s) 143, 416, 1804
BseGI GGATG 4 cut(s) 1255, 1918, 1920, 1922
BseJI GATNNNNATC 1 cut(s) 465
BseLI CCNNNNNNNGG 5 cut(s) 71, 851, 970, 1127, 1810
BseMI GCAATG 2 cut(s) 363, 1436
BseMII CTCAG 4 cut(s) 627, 1251, 1520, 1733
BseNI ACTGG 5 cut(s) 96, 481, 856, 1175, 1906
BseRI GAGGAG 3 cut(s) 467, 1259, 1298
BseSI GKGCMC 2 cut(s) 142, 152
BseXI GCAGC 8 cut(s) 695, 760, 965, 1198, 1441, 1537, 1702, 1841
BsgI GTGCAG 3 cut(s) 191, 694, 925
Bsh1236I CGCG 1 cut(s) 145
BshFI GGCC 1 cut(s) 412
BsiHKAI GWGCWC 4 cut(s) 53, 142, 152, 925
BslFI GGGAC 1 cut(s) 1893
BslI CCNNNNNNNGG 5 cut(s) 71, 851, 970, 1127, 1810
BsmAI GTCTC 2 cut(s) 804, 1769
BsmFI GGGAC 1 cut(s) 1893
BsmI GAATGC 1 cut(s) 1234
BsnI GGCC 1 cut(s) 412
Bso31I GGTCTC 1 cut(s) 1769
Bsp1286I GDGCHC 4 cut(s) 53, 142, 152, 925
Bsp1407I TGTACA 2 cut(s) 1347, 1541
Bsp143I GATC 7 cut(s) 43, 177, 472, 968, 1084, 1171, 1252
Bsp1720I GCTNAGC 1 cut(s) 1719
BspACI CCGC 6 cut(s) 143, 145, 159, 996, 1644, 1768
BspANI GGCC 1 cut(s) 412
BspCNI CTCAG 4 cut(s) 626, 1252, 1521, 1732
BspFNI CGCG 1 cut(s) 145
BspLI GGNNCC 4 cut(s) 179, 840, 962, 1119
BspMAI CTGCAG 1 cut(s) 778
BspMI ACCTGC 1 cut(s) 1902
BspPI GGATC 4 cut(s) 38, 172, 185, 1166
BspTNI GGTCTC 1 cut(s) 1769
BsrBI CCGCTC 1 cut(s) 161
BsrDI GCAATG 2 cut(s) 363, 1436
BsrGI TGTACA 2 cut(s) 1347, 1541
BsrI ACTGG 5 cut(s) 96, 481, 856, 1175, 1906
BssECI CCNNGG 3 cut(s) 143, 416, 1804
BssMI GATC 7 cut(s) 43, 177, 472, 968, 1084, 1171, 1252
BssT1I CCWWGG 1 cut(s) 416
Bst2UI CCWGG 2 cut(s) 846, 1806
Bst4CI ACNGT 4 cut(s) 199, 495, 788, 1510
Bst6I CTCTTC 1 cut(s) 612
BstAPI GCANNNNNTGC 1 cut(s) 1835
BstAUI TGTACA 2 cut(s) 1347, 1541
BstC8I GCNNGC 4 cut(s) 53, 526, 1627, 1869
BstDEI CTNAG 5 cut(s) 613, 1260, 1520, 1529, 1719
BstDSI CCRYGG 1 cut(s) 143
BstF5I GGATG 4 cut(s) 1255, 1918, 1920, 1922
BstFNI CGCG 1 cut(s) 145
BstHHI GCGC 1 cut(s) 889
BstKTI GATC 7 cut(s) 46, 180, 475, 971, 1087, 1174, 1255
BstMAI GTCTC 2 cut(s) 804, 1769
BstMBI GATC 7 cut(s) 43, 177, 472, 968, 1084, 1171, 1252
BstMWI GCNNNNNNNGC 2 cut(s) 440, 1835
BstNI CCWGG 2 cut(s) 846, 1806
BstNSI RCATGY 2 cut(s) 287, 921
BstPAI GACNNNNGTC 1 cut(s) 587
BstSCI CCNGG 2 cut(s) 844, 1804
BstSFI CTRYAG 1 cut(s) 774
BstSLI GKGCMC 2 cut(s) 142, 152
BstUI CGCG 1 cut(s) 145
BstV1I GCAGC 8 cut(s) 695, 760, 965, 1198, 1441, 1537, 1702, 1841
BstV2I GAAGAC 1 cut(s) 80
BstX2I RGATCY 1 cut(s) 177
BstYI RGATCY 1 cut(s) 177
Bsu36I CCTNAGG 1 cut(s) 1520
BsuRI GGCC 1 cut(s) 412
BtgI CCRYGG 1 cut(s) 143
BtsCI GGATG 4 cut(s) 1255, 1918, 1920, 1922
BtsIMutI CAGTG 6 cut(s) 150, 204, 1182, 1321, 1506, 1550
BveI ACCTGC 1 cut(s) 1902
Cac8I GCNNGC 4 cut(s) 53, 526, 1627, 1869
CfoI GCGC 1 cut(s) 889
Cfr13I GGNCC 2 cut(s) 839, 1321
Cfr42I CCGCGG 1 cut(s) 146
Csp6I GTAC 6 cut(s) 103, 849, 1348, 1542, 1641, 1909
CspCI CAANNNNNGTGG 2 cut(s) 916, 951
CviAII CATG 9 cut(s) 71, 248, 284, 313, 529, 918, 1396, 1772, 1810
CviQI GTAC 6 cut(s) 103, 849, 1348, 1542, 1641, 1909
DdeI CTNAG 5 cut(s) 613, 1260, 1520, 1529, 1719
DpnI GATC 7 cut(s) 45, 179, 474, 970, 1086, 1173, 1254
DpnII GATC 7 cut(s) 43, 177, 472, 968, 1084, 1171, 1252
DriI GACNNNNNGTC 1 cut(s) 870
Eam1104I CTCTTC 1 cut(s) 612
Eam1105I GACNNNNNGTC 1 cut(s) 870
EarI CTCTTC 1 cut(s) 612
EciI GGCGGA 1 cut(s) 1757
Ecl136II GAGCTC 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 416
Eco147I AGGCCT 1 cut(s) 412
Eco24I GRGCYC 1 cut(s) 53
Eco31I GGTCTC 1 cut(s) 1769
Eco32I GATATC 1 cut(s) 361
Eco47I GGWCC 2 cut(s) 839, 1321
Eco53kI GAGCTC 1 cut(s) 51
Eco57I CTGAAG 2 cut(s) 1346, 1574
Eco81I CCTNAGG 1 cut(s) 1520
EcoICRI GAGCTC 1 cut(s) 51
EcoO109I RGGNCCY 2 cut(s) 839, 1321
EcoRI GAATTC 2 cut(s) 817, 1571
EcoRII CCWGG 2 cut(s) 844, 1804
EcoRV GATATC 1 cut(s) 361
EcoT14I CCWWGG 1 cut(s) 416
EcoT22I ATGCAT 4 cut(s) 289, 395, 1600, 1920
EcoT38I GRGCYC 1 cut(s) 53
ErhI CCWWGG 1 cut(s) 416
FaeI CATG 9 cut(s) 74, 251, 287, 316, 532, 921, 1399, 1775, 1813
FaqI GGGAC 1 cut(s) 1893
FatI CATG 9 cut(s) 70, 247, 283, 312, 528, 917, 1395, 1771, 1809
FauI CCCGC 1 cut(s) 989
FbaI TGATCA 1 cut(s) 1084
Fnu4HI GCNGC 8 cut(s) 709, 774, 954, 1212, 1455, 1526, 1716, 1830
FokI GGATG 4 cut(s) 1262, 1905, 1909, 1927
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 8 cut(s) 709, 774, 954, 1212, 1455, 1526, 1716, 1830
FspBI CTAG 5 cut(s) 714, 755, 944, 1007, 1616
GlaI GCGC 1 cut(s) 888
GluI GCNGC 8 cut(s) 709, 774, 954, 1212, 1455, 1526, 1716, 1830
GsuI CTGGAG 3 cut(s) 79, 375, 464
HaeIII GGCC 1 cut(s) 412
HhaI GCGC 1 cut(s) 889
Hin1II CATG 9 cut(s) 74, 251, 287, 316, 532, 921, 1399, 1775, 1813
Hin6I GCGC 1 cut(s) 887
HinP1I GCGC 1 cut(s) 887
HphI GGTGA 2 cut(s) 9, 803
Hpy166II GTNNAC 3 cut(s) 140, 150, 1350
Hpy188III TCNNGA 4 cut(s) 332, 470, 755, 1082
Hpy8I GTNNAC 3 cut(s) 140, 150, 1350
Hpy99I CGWCG 1 cut(s) 87
HpyAV CCTTC 3 cut(s) 237, 595, 1457
HpyCH4III ACNGT 4 cut(s) 199, 495, 788, 1510
HpyCH4IV ACGT 1 cut(s) 378
HpyF10VI GCNNNNNNNGC 2 cut(s) 440, 1835
HpyF3I CTNAG 5 cut(s) 613, 1260, 1520, 1529, 1719
HpySE526I ACGT 1 cut(s) 378
Hsp92II CATG 9 cut(s) 74, 251, 287, 316, 532, 921, 1399, 1775, 1813
HspAI GCGC 1 cut(s) 887
Ksp22I TGATCA 1 cut(s) 1084
KspI CCGCGG 1 cut(s) 146
Kzo9I GATC 7 cut(s) 43, 177, 472, 968, 1084, 1171, 1252
LmnI GCTCC 4 cut(s) 166, 749, 928, 966
Lsp1109I GCAGC 8 cut(s) 695, 760, 965, 1198, 1441, 1537, 1702, 1841
LweI GCATC 5 cut(s) 296, 340, 1655, 1905, 1927
MaeI CTAG 5 cut(s) 714, 755, 944, 1007, 1616
MaeII ACGT 1 cut(s) 378
MaeIII GTNAC 6 cut(s) 113, 199, 206, 981, 1028, 1070
MalI GATC 7 cut(s) 45, 179, 474, 970, 1086, 1173, 1254
MbiI CCGCTC 1 cut(s) 161
MboI GATC 7 cut(s) 43, 177, 472, 968, 1084, 1171, 1252
MboII GAAGA 9 cut(s) 80, 629, 978, 1057, 1306, 1339, 1425, 1647, 1860
MflI RGATCY 1 cut(s) 177
MhlI GDGCHC 4 cut(s) 53, 142, 152, 925
MlyI GAGTC 1 cut(s) 880
MmeI TCCRAC 1 cut(s) 56
Mph1103I ATGCAT 4 cut(s) 289, 395, 1600, 1920
MroXI GAANNNNTTC 1 cut(s) 218
MseI TTAA 8 cut(s) 639, 830, 975, 1428, 1601, 1631, 1725, 1815
MslI CAYNNNNRTG 1 cut(s) 145
MspA1I CMGCKG 2 cut(s) 145, 1528
MspR9I CCNGG 2 cut(s) 846, 1806
Mva1269I GAATGC 1 cut(s) 1234
MvaI CCWGG 2 cut(s) 846, 1806
MvnI CGCG 1 cut(s) 145
MwoI GCNNNNNNNGC 2 cut(s) 440, 1835
NdeII GATC 7 cut(s) 43, 177, 472, 968, 1084, 1171, 1252
NlaIII CATG 9 cut(s) 74, 251, 287, 316, 532, 921, 1399, 1775, 1813
NlaIV GGNNCC 4 cut(s) 179, 840, 962, 1119
NmuCI GTSAC 3 cut(s) 199, 981, 1070
NsiI ATGCAT 4 cut(s) 289, 395, 1600, 1920
NspI RCATGY 2 cut(s) 287, 921
OliI CACNNNNGTG 1 cut(s) 145
PaqCI CACCTGC 1 cut(s) 1902
PceI AGGCCT 1 cut(s) 412
PciI ACATGT 1 cut(s) 917
PcsI WCGNNNNNNNCGW 1 cut(s) 82
PctI GAATGC 1 cut(s) 1234
PdmI GAANNNNTTC 1 cut(s) 218
PfeI GAWTC 9 cut(s) 24, 155, 265, 274, 317, 674, 761, 1307, 1736
PkrI GCNGC 8 cut(s) 710, 775, 955, 1213, 1456, 1527, 1717, 1831
PleI GAGTC 1 cut(s) 879
PpsI GAGTC 1 cut(s) 879
PpuMI RGGWCCY 2 cut(s) 839, 1321
PscI ACATGT 1 cut(s) 917
PshAI GACNNNNGTC 1 cut(s) 587
PshBI ATTAAT 1 cut(s) 830
PsiI TTATAA 1 cut(s) 572
Psp124BI GAGCTC 1 cut(s) 53
Psp1406I AACGTT 1 cut(s) 378
Psp5II RGGWCCY 2 cut(s) 839, 1321
Psp6I CCWGG 2 cut(s) 844, 1804
PspGI CCWGG 2 cut(s) 844, 1804
PspN4I GGNNCC 4 cut(s) 179, 840, 962, 1119
PspPI GGNCC 2 cut(s) 839, 1321
PspPPI RGGWCCY 2 cut(s) 839, 1321
PsrI GAACNNNNNNTAC 2 cut(s) 1525, 1557
PstI CTGCAG 1 cut(s) 778
PsuI RGATCY 1 cut(s) 177
PvuII CAGCTG 1 cut(s) 1528
RsaI GTAC 6 cut(s) 104, 850, 1349, 1543, 1642, 1910
RsaNI GTAC 6 cut(s) 103, 849, 1348, 1542, 1641, 1909
RseI CAYNNNNRTG 1 cut(s) 145
SacI GAGCTC 1 cut(s) 53
SacII CCGCGG 1 cut(s) 146
SaqAI TTAA 8 cut(s) 639, 830, 975, 1428, 1601, 1631, 1725, 1815
SatI GCNGC 8 cut(s) 709, 774, 954, 1212, 1455, 1526, 1716, 1830
Sau3AI GATC 7 cut(s) 43, 177, 472, 968, 1084, 1171, 1252
Sau96I GGNCC 2 cut(s) 839, 1321
ScaI AGTACT 1 cut(s) 104
SchI GAGTC 1 cut(s) 880
ScrFI CCNGG 2 cut(s) 846, 1806
SduI GDGCHC 4 cut(s) 53, 142, 152, 925
SfaNI GCATC 5 cut(s) 296, 340, 1655, 1905, 1927
SfcI CTRYAG 1 cut(s) 774
Sfr303I CCGCGG 1 cut(s) 146
SgrBI CCGCGG 1 cut(s) 146
SinI GGWCC 2 cut(s) 839, 1321
SmiMI CAYNNNNRTG 1 cut(s) 145
SmlI CTYRAG 1 cut(s) 986
SmoI CTYRAG 1 cut(s) 986
SseBI AGGCCT 1 cut(s) 412
SsiI CCGC 6 cut(s) 143, 145, 159, 996, 1644, 1768
SspI AATATT 2 cut(s) 934, 1635
SspMI CTAG 5 cut(s) 714, 755, 944, 1007, 1616
SstI GAGCTC 1 cut(s) 53
StuI AGGCCT 1 cut(s) 412
StyD4I CCNGG 2 cut(s) 844, 1804
StyI CCWWGG 1 cut(s) 416
TaaI ACNGT 4 cut(s) 199, 495, 788, 1510
TaiI ACGT 1 cut(s) 381
TaqI TCGA 5 cut(s) 22, 263, 500, 1255, 1380
TatI WGTACW 4 cut(s) 102, 1347, 1541, 1908
TfiI GAWTC 9 cut(s) 24, 155, 265, 274, 317, 674, 761, 1307, 1736
Tru1I TTAA 8 cut(s) 639, 830, 975, 1428, 1601, 1631, 1725, 1815
Tru9I TTAA 8 cut(s) 639, 830, 975, 1428, 1601, 1631, 1725, 1815
TscAI CASTG 6 cut(s) 157, 204, 1182, 1321, 1513, 1557
TseFI GTSAC 3 cut(s) 199, 981, 1070
TseI GCWGC 8 cut(s) 708, 773, 953, 1211, 1454, 1525, 1715, 1829
Tsp45I GTSAC 3 cut(s) 199, 981, 1070
TspDTI ATGAA 8 cut(s) 211, 339, 477, 545, 1058, 1314, 1425, 1476
TspGWI ACGGA 3 cut(s) 101, 554, 581
TspRI CASTG 6 cut(s) 157, 204, 1182, 1321, 1513, 1557
VneI GTGCAC 2 cut(s) 138, 148
VpaK11BI GGWCC 2 cut(s) 839, 1321
VspI ATTAAT 1 cut(s) 830
XapI RAATTY 5 cut(s) 300, 717, 817, 1301, 1571
XbaI TCTAGA 1 cut(s) 754
XceI RCATGY 2 cut(s) 287, 921
XmnI GAANNNNTTC 1 cut(s) 218
XspI CTAG 5 cut(s) 714, 755, 944, 1007, 1616
ZrmI AGTACT 1 cut(s) 104
Zsp2I ATGCAT 4 cut(s) 289, 395, 1600, 1920
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.