Rroxscaffold_6G00394280

AAA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000279
Physical Location & Seq
Reverse (-)
14645820 .. 14653699
7880 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00394280.1

Sequence Viewer

Length: 3492 bp
ATGTCGGACCTCCACCGCCGGTGCTCGTCCCCACCATTGGACCACCGAGAGGATGATGCCATCGAACTCCACGATCCCCACCCCTCTAAGCTTCACCGATTTTGTTCGTCAAATGTCCGGGAAGCTGCGAAGCTCCAAGCGGGAATCGGGTCCGGGCTTTGCCGGTTTTGTTTGTCGGATGTCCGGGAAGCTCCGAAGCTCCACGCCGGAATCGAGTCCGGGCTTCGCCGGTTTTGTTCGTCGGATGTCCGGGAAGCTCCGAACCTCCACGCCGGAATCGGGGATGTTCTCAATGAAAACCTTTACAGAAATCAGGTGCAGAGGATCCCCGACACATTTGTGACATTGACGAGTTACAAGAAATCATTCATTCCTTCACTTGTCGAGGAAACTCATGCTGATTTACTATCGAATATGTTGAATCTGTCACAAGCACCTACTTGTGAAATTCTGACAATCGAAGATTCAGATGGAACTCCCGGTGACTTGCTTTACGATGTAACATATCAGAGAGATACAGAAACTGATGACAATCACAAAGGACTGATGTATGAGCCACAGGTTGGAGATATCATCGCCTTGACTAATGTTAGACCAAAATGCATTGATGATTTGAACAGGCCTCCAAGGTTCTATCTAATCGCTTATGTTGATAAAGCAAATGATATTGAAGAATTTCCTGATGATCTCCAATTCAAAATACTATCATCAAAGCCTATCAACTATGGAGAACCAGACATGCATAAGAGCAAGAGAGAGACACTTTTTGCTGTCTATCTTATGAACTTGACAACAAATCTCCGTGTATGGAAGGCTTTGAACTCAGAGGGAAATACAAATATCATTAACAAAGTTCTGCAACCCAAGTCAGATGATGGGGATTCTTGTTCAGTTTGCTTCTCCAAACAAAAATGCAATGCTGGCATTTCTGCATTATGGCCTACAATTTGCTCTCAAAATCTAAATGAATCCCAAGAAGCTGCAGTTTTGAACTGTATCAGTTTGACTCAATGCCATCACCAGAATTCTGTAAAATTAATATGGGGTCCTCCTGGTACTGGCAAGACGAAGACAATGAGTCTGACACTCTTTGCTCTCTTTCAGTTAAAGTGCAGAACTCTCACATGTGCTCCCACCAATATTGCGGTGCTAGAAGTAGCAGCAAGACTCCGGAGATTGGTTAACCAGTCACTCGAGTATGGCAGGTATGGACTAGGAGATATAGTTCTCTTTGGTAACAAGAAGCGAATGAAGATTGACAATAATGATGATCTTCGTGATATATTTCTTGATCATCGTGTTAAAATTCTCATCAAGTGTTTGGTACCTTTATCTGGGTGGAAACATTTGTTAGAATCAATGATACATTTACTTGATGATCCTGAGGAACAATATTCTTTGTACTTGGAAAAGAGAGCTAAAGAGCAGAAACAGAATGCACAAAAACATGAGGAGGATAATCAAAAACATAATGGAGATGAGGATTATCCTTTGACATATGAGGAGTTTGTAAAGAATGAATTTGATTCAATCAGCCAGGAGCTGAAGGTTTGTATGGTAGATTTGTACACCCACTTACCAACTTGTTGCATTTCACTTAAAGTGGTGAAGGACATGGTCAGAGCTTTGGGTTTGCTCAAGTCTATTAAATCTTCATTGCATACTACTGTTGTTGCTAATGAAGGGTTGAAGTTAGTCTTCAAAGATTCCAAAGTTCCAGGAAGCATTGTTGGTTGCCTTACGCAGTTGAGAACAGAGTGTACAAACATACTGAAGTCGCTTCCTATTAACAAATATGCATTAATGAACTTCTGCCTAGAAAATGCTTGCTTAATATTCTGTACTGCATCAACTTCTTCCAAATTGCATGTTGTAGCAGGAACAAGGCCATTGGAATTGTTGGTCATTGATGAAGCTGCTCAGCTTAAAGAATGTGAATCAGCAATTCCTTTACAACTATCTGGTCTCCGCCATGCTATCCTTGTAGGAGATGAGAGGCAACTCCCTGCCATGGTTAAAAGCGAGGCTGGCAAAGTTGGGGCACAAGAAGCACCTACTCAATGTCCAGTACAGGATGCATCCATCCATCAGTTTATTTCCAAAAGGGAGTTCTACGACAACCAGATTGTAGATGGTCCAAATGTCAAAGAAAGAAGCTATGAAAGGTGCTTCCTCAAGGGAAAAATATACCAATCCTATTCCTTCATAAATGTAGCCGATGGAAAAGAAGAATTTGATCACAGATTTAGTCAGAAAAATATGGTGGAGGTTGCTGTAGTCTCTGGGATAGTAGCAAGCCTTTATAAAGAATTTATTGGAACAAAGAAGAAAGTTAGTATTGGGGTCATATCACCATACAAGGCTCAAGTTTATGCAATTCAAGAGATACTCAGAAAATATACTGAAACTTCTGATACTGGATTCTCTGTAAGTGTGCGATCTGTTGATGGGTTCCAAGGTGGTGAAGAAGATGTGATAATTATATCTACTGTCCGATGTAATGGGAATGGGTCAGTTGGTTTCTTGTCGAACCGGCAAAGAGCAAATGTTGCCTTAACACGTGCAAGGTATTGCCTTTGGATATTGGGGAATGCGTCAACTTTGATTAGTAGTGACTCTGTTTGGAAGAAGCTAGTCCTTGATGCCAAGAAACGGAACTGTTTTTATAATGCTGATGAAGACAGCGACTTGGCCCAGGCTATTACAGCAGCCCTGCTCAAGCTTGACCAACTTCATTCTCTGCTTAATATCGACTCTATGCTGTTCAAAAATGCTATATGGAAGGTTTGCTTCACTGATGACTTTCGGAACTCCATAGCAAAAATTAAAGACACTGTGATTCTTCAGGAAGTACTCGCGTTATTAACCAAGCTTTTGAGTGGATGGCGCCGACCTCACAAGGATAAAGGAAATTTAGTGTACGATGGGACTTCTGCTCAACTGTTAGAGAAGTATAAAATCAAAGGGCACTTGAATCTCATTTGGACTGTAGATATTCTCCAGGAGAATGCACATTACATCCAAGTTATGAAGTTTTGGGATATTTTGCCATTTTCTCATATACCAGAACTAGCAAAGCGTCTTGACATTGTTTTTGGAAATTTTACAGTGGATAAGATGAACCGCTGCAGGCACAAATGCATTGACAGGGATACTGTTGTTCCAATGAGATGGCCAGTGGTTTTCAGAAATTTTCCTACAGCTGATCATGAGGAGTTCCTTTCAAAACCATTGTCTTCTTTCTGTATAACAACTAATCGAGAAACAGCAACTTCAACATATGGGGAGACAGCGAAAGCAGTAAAACCGATTATCCGTTCGAAAAGCAACGTCAACAAAAAAGAAAGAAGGTTATGGAAAATAAAGGCTGAAGACAAAGTGAAGGAGTTTAGCACTTGCCTTGAAGCTGACCCTGTGGAGTTCTTTTCAAAACCATTATCATCACTAAGTATAGCAGAGAAGCCAGAAGCATCTACTTCCACTGACACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12

Protein Analysis

1163

Amino Acids

131.13

Weight (kDa)

7.46

Isoelectric Point (pI)

40.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 146 - 278 7.3e-14 Domain of unknown function (DUF6469)
AAA_11 PF13086 320 - 675 1.5e-33 AAA domain
AAA_12 PF13087 694 - 865 1.5e-42 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000279)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65810 AT1G65810 AT1G65810 AT4G05540 AT5G37140 AT5G37150 AT5G37160 AT5G37165 AT5G52090
fragaria_vesca FvH4_1g27541 FvH4_1g27542 FvH4_2g08960 FvH4_2g08960 FvH4_2g08960 FvH4_2g08960 FvH4_3g00170 FvH4_3g00170 FvH4_3g00170 FvH4_5g05930 FvH4_5g05930
malus_domestica MD08G1077700.v1.1 MD08G1077900.v1.1 MD08G1078000.v1.1 MD08G1078100.v1.1 MD08G1078300.v1.1 MD09G1057800.v1.1 MD09G1281400.v1.1 MD11G1222900.v1.1 MD14G1146400.v1.1
prunus_persica Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G202100_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.3G013100_v2.0.a1
pyrus_communis pycom09g18900 pycom09g18910 pycom13g26720 pycom13g26730 pycom13g26760 pycom13g26770
rosa_chinensis RchiOBHm_Chr3g0488001 RchiOBHm_Chr3g0488011 RchiOBHm_Chr3g0488031 RchiOBHm_Chr3g0488061 RchiOBHm_Chr3g0488111 RchiOBHm_Chr3g0488201 RchiOBHm_Chr3g0488211 RchiOBHm_Chr3g0488221 RchiOBHm_Chr3g0488321 RchiOBHm_Chr3g0488431 RchiOBHm_Chr6g0264601 RchiOBHm_Chr6g0264641
rosa_laevigata RLG00000014240 RLG00000014241 RLG00000022910 RLG00000022949 RLG00000030376
rosa_multiflora Rmu_co8075388.1_g000001 Rmu_co8424869.1_g000001 Rmu_co8427783.1_g000001 Rmu_sc0000546.1_g000002 Rmu_sc0000546.1_g000015 Rmu_sc0011052.1_g000014 Rmu_sc0017772.1_g000004
rosa_roxburghii Rroxscaffold_175G00431980 Rroxscaffold_175G00431990 Rroxscaffold_1G00034600 Rroxscaffold_6G00394230 Rroxscaffold_6G00394270 Rroxscaffold_6G00394280 Rroxscaffold_6G00394350 Rroxscaffold_7G00203240 Rroxscaffold_7G00203270 Rroxscaffold_7G00203280 Rroxscaffold_7G00203300 Rroxscaffold_7G00205740
rosa_rugosa Rorug03G0238000 Rorug03G0238100 Rorug03G0238100 Rorug03G0238500 Rorug05G0155400 Rorug05G0356000 Rorug05G0463000 Rorug06G0015200 Rorug06G0015300 Rorug06G0015400 Rorug06G0015500 Rorug06G0015500
rosa_samantha Rh1DG088100 Rh2AG244800 Rh2BG187500 Rh2CG247300 Rh3BG322700 Rh3BG322800 Rh3BG323100 Rh3BG323900 Rh3BG324000 Rh3BG324300 Rh3CG321300 Rh3CG321400 Rh3CG321500 Rh3DG319400 Rh3DG319900 Rh3DG320100 Rh3DG320200 Rh6AG133700 Rh6AG133800 Rh6AG133900 Rh6CG129300 Rh6CG129800 Rh6CG130200 Rh6DG116500 Rh6DG117200 Rh7DG378000
rosa_wichuraiana Rw2G014710 Rw3G025340 Rw3G025350 Rw3G025360 Rw5G020630 Rw6G011590 Rw6G011600 Rw7G041380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2304, 2667
Acc36I ACCTGC 1 cut(s) 1194
Acc65I GGTACC 1 cut(s) 1324
AccB1I GGYRCC 2 cut(s) 1324, 2886
AccB7I CCANNNNNTGG 1 cut(s) 563
AccII CGCG 1 cut(s) 2858
AccIII TCCGGA 1 cut(s) 1170
AciI CCGC 5 cut(s) 16, 140, 1145, 1969, 3124
AclWI GGATC 4 cut(s) 68, 319, 332, 1373
AcoI YGGCCR 1 cut(s) 3173
AcuI CTGAAG 4 cut(s) 1565, 1793, 2828, 3390
AcvI CACGTG 2 cut(s) 2561, 3489
AcyI GRCGYC 1 cut(s) 2887
AfaI GTAC 9 cut(s) 1057, 1326, 1403, 1568, 1762, 1843, 2070, 2853, 2921
AfiI CCNNNNNNNGG 9 cut(s) 37, 49, 279, 563, 1058, 1177, 1334, 2011, 2652
AflIII ACRYGT 3 cut(s) 1124, 2558, 3486
AhdI GACNNNNNGTC 1 cut(s) 1077
AjnI CCWGG 5 cut(s) 1051, 1536, 1717, 2694, 3000
AjuI GAANNNNNNNTTGG 2 cut(s) 2093, 2125
AleI CACNNNNGTG 1 cut(s) 338
AloI GAACNNNNNNTCC 2 cut(s) 1209, 1241
Alw21I GWGCWC 2 cut(s) 26, 1132
Alw26I GTCTC 4 cut(s) 752, 1970, 2284, 3281
AlwI GGATC 4 cut(s) 68, 319, 332, 1373
AlwNI CAGNNNCTG 2 cut(s) 524, 1543
Ama87I CYCGRG 1 cut(s) 1193
Aor13HI TCCGGA 1 cut(s) 1170
AoxI GGCC 5 cut(s) 620, 938, 1886, 2691, 3173
ApeKI GCWGC 6 cut(s) 125, 980, 1160, 1916, 2708, 3126
ArsI GACNNNNNNTTYG 4 cut(s) 3077, 3109, 3218, 3250
AseI ATTAAT 2 cut(s) 1037, 1802
Asp700I GAANNNNTTC 2 cut(s) 365, 675
Asp718I GGTACC 1 cut(s) 1324
AspLEI GCGC 1 cut(s) 2889
AspS9I GGNCC 6 cut(s) 7, 40, 150, 1046, 2135, 2692
AsuC2I CCSGG 6 cut(s) 119, 154, 185, 220, 251, 480
AsuHPI GGTGA 6 cut(s) 86, 494, 1010, 1618, 2343, 2474
AsuII TTCGAA 1 cut(s) 3320
AvaI CYCGRG 1 cut(s) 1193
AvaII GGWCC 5 cut(s) 7, 40, 150, 1046, 2135
AxyI CCTNAGG 1 cut(s) 1383
BaeGI GKGCMC 2 cut(s) 2044, 2970
BaeI ACNNNNGTAYC 2 cut(s) 3144, 3177
BalI TGGCCA 1 cut(s) 3175
BamHI GGATCC 1 cut(s) 324
BanI GGYRCC 2 cut(s) 1324, 2886
BbrPI CACGTG 2 cut(s) 2561, 3489
BbsI GAAGAC 5 cut(s) 1076, 1690, 2685, 3228, 3378
Bbv12I GWGCWC 2 cut(s) 26, 1132
BbvI GCAGC 6 cut(s) 112, 967, 1172, 1903, 2720, 3113
BciT130I CCWGG 5 cut(s) 1053, 1538, 1719, 2696, 3002
BciVI GTATCC 1 cut(s) 3145
BclI TGATCA 3 cut(s) 1291, 2236, 3205
BcnI CCSGG 6 cut(s) 119, 154, 185, 220, 251, 480
BcoDI GTCTC 4 cut(s) 752, 1970, 2284, 3281
BfaI CTAG 5 cut(s) 1151, 1214, 1817, 2633, 3071
BfmI CTRYAG 5 cut(s) 981, 2274, 2988, 3127, 3198
BfoI RGCGCY 1 cut(s) 2890
BfuAI ACCTGC 1 cut(s) 1194
BfuI GTATCC 1 cut(s) 3145
BisI GCNGC 6 cut(s) 126, 981, 1161, 1917, 2709, 3127
BlpI GCTNAGC 1 cut(s) 1920
BlsI GCNGC 6 cut(s) 127, 982, 1162, 1918, 2710, 3128
BmcAI AGTACT 1 cut(s) 2853
Bme18I GGWCC 5 cut(s) 7, 40, 150, 1046, 2135
BmeRI GACNNNNNGTC 1 cut(s) 1077
BmeT110I CYCGRG 1 cut(s) 1193
BmgT120I GGNCC 6 cut(s) 7, 40, 150, 1046, 2135, 2692
BmiI GGNNCC 6 cut(s) 151, 326, 1047, 1326, 2453, 2888
BmsI GCATC 6 cut(s) 46, 1856, 2065, 2087, 2632, 3479
BpiI GAAGAC 5 cut(s) 1076, 1690, 2685, 3228, 3378
BpmI CTGGAG 1 cut(s) 2984
Bpu1102I GCTNAGC 1 cut(s) 1920
Bpu14I TTCGAA 1 cut(s) 3320
BpuEI CTTGAG 4 cut(s) 1622, 2159, 2349, 2702
BpuMI CCSGG 6 cut(s) 119, 154, 185, 220, 251, 480
BsaAI YACGTR 2 cut(s) 2561, 3489
BsaBI GATNNNNATC 1 cut(s) 531
BsaHI GRCGYC 1 cut(s) 2887
BsaI GGTCTC 1 cut(s) 1970
BsaJI CCNNGG 4 cut(s) 626, 2010, 2455, 2694
BsaWI WCCGGW 1 cut(s) 1170
BsaXI ACNNNNNCTCC 4 cut(s) 1114, 1144, 1209, 1239
Bsc4I CCNNNNNNNGG 9 cut(s) 37, 49, 279, 563, 1058, 1177, 1334, 2011, 2652
Bse118I RCCGGY 4 cut(s) 18, 162, 228, 2532
Bse1I ACTGG 5 cut(s) 1063, 1186, 2066, 2422, 3176
Bse21I CCTNAGG 1 cut(s) 1383
Bse3DI GCAATG 2 cut(s) 922, 1655
Bse8I GATNNNNATC 1 cut(s) 531
BseAI TCCGGA 1 cut(s) 1170
BseBI CCWGG 5 cut(s) 1053, 1538, 1719, 2696, 3002
BseDI CCNNGG 4 cut(s) 626, 2010, 2455, 2694
BseGI GGATG 9 cut(s) 58, 184, 250, 289, 2078, 2080, 2082, 2888, 3018
BseJI GATNNNNATC 1 cut(s) 531
BseLI CCNNNNNNNGG 9 cut(s) 37, 49, 279, 563, 1058, 1177, 1334, 2011, 2652
BseMI GCAATG 2 cut(s) 922, 1655
BseMII CTCAG 4 cut(s) 837, 1374, 1934, 2404
BseNI ACTGG 5 cut(s) 1063, 1186, 2066, 2422, 3176
BseRI GAGGAG 3 cut(s) 1466, 1517, 3227
BseSI GKGCMC 2 cut(s) 2044, 2970
BseXI GCAGC 6 cut(s) 112, 967, 1172, 1903, 2720, 3113
BsgI GTGCAG 2 cut(s) 338, 1132
Bsh1236I CGCG 1 cut(s) 2858
BshFI GGCC 5 cut(s) 622, 940, 1888, 2693, 3175
BshNI GGYRCC 2 cut(s) 1324, 2886
BsiHKAI GWGCWC 2 cut(s) 26, 1132
BsiHKCI CYCGRG 1 cut(s) 1193
BslFI GGGAC 2 cut(s) 13, 2941
BslI CCNNNNNNNGG 9 cut(s) 37, 49, 279, 563, 1058, 1177, 1334, 2011, 2652
BsmAI GTCTC 4 cut(s) 752, 1970, 2284, 3281
BsmFI GGGAC 2 cut(s) 13, 2941
BsmI GAATGC 3 cut(s) 1441, 2596, 3013
BsnI GGCC 5 cut(s) 622, 940, 1888, 2693, 3175
Bso31I GGTCTC 1 cut(s) 1970
BsoBI CYCGRG 1 cut(s) 1193
Bsp119I TTCGAA 1 cut(s) 3320
Bsp1286I GDGCHC 4 cut(s) 26, 1132, 2044, 2970
Bsp13I TCCGGA 1 cut(s) 1170
Bsp1407I TGTACA 2 cut(s) 1566, 1760
Bsp143I GATC 9 cut(s) 73, 324, 685, 1270, 1291, 1378, 2236, 2438, 3205
Bsp1720I GCTNAGC 1 cut(s) 1920
Bsp19I CCATGG 1 cut(s) 2010
BspACI CCGC 5 cut(s) 16, 140, 1145, 1969, 3124
BspANI GGCC 5 cut(s) 622, 940, 1888, 2693, 3175
BspCNI CTCAG 4 cut(s) 836, 1375, 1933, 2403
BspEI TCCGGA 1 cut(s) 1170
BspFNI CGCG 1 cut(s) 2858
BspHI TCATGA 1 cut(s) 3208
BspLI GGNNCC 6 cut(s) 151, 326, 1047, 1326, 2453, 2888
BspMAI CTGCAG 2 cut(s) 985, 3131
BspMI ACCTGC 1 cut(s) 1194
BspPI GGATC 4 cut(s) 68, 319, 332, 1373
BspT104I TTCGAA 1 cut(s) 3320
BspT107I GGYRCC 2 cut(s) 1324, 2886
BspTNI GGTCTC 1 cut(s) 1970
BsrDI GCAATG 2 cut(s) 922, 1655
BsrFI RCCGGY 4 cut(s) 18, 162, 228, 2532
BsrGI TGTACA 2 cut(s) 1566, 1760
BsrI ACTGG 5 cut(s) 1063, 1186, 2066, 2422, 3176
BssAI RCCGGY 4 cut(s) 18, 162, 228, 2532
BssECI CCNNGG 4 cut(s) 626, 2010, 2455, 2694
BssMI GATC 9 cut(s) 73, 324, 685, 1270, 1291, 1378, 2236, 2438, 3205
BssNI GRCGYC 1 cut(s) 2887
BssT1I CCWWGG 3 cut(s) 626, 2010, 2455
Bst2UI CCWGG 5 cut(s) 1053, 1538, 1719, 2696, 3002
Bst4CI ACNGT 9 cut(s) 995, 1669, 2491, 2660, 2836, 2943, 2989, 3109, 3157
BstACI GRCGYC 1 cut(s) 2887
BstAPI GCANNNNNTGC 1 cut(s) 2549
BstAUI TGTACA 2 cut(s) 1566, 1760
BstBAI YACGTR 2 cut(s) 2561, 3489
BstBI TTCGAA 1 cut(s) 3320
BstC8I GCNNGC 5 cut(s) 922, 1828, 2029, 2296, 3131
BstDEI CTNAG 6 cut(s) 87, 823, 1383, 1920, 2390, 3446
BstDSI CCRYGG 1 cut(s) 2010
BstF5I GGATG 9 cut(s) 58, 184, 250, 289, 2078, 2080, 2082, 2888, 3018
BstFNI CGCG 1 cut(s) 2858
BstH2I RGCGCY 1 cut(s) 2890
BstHHI GCGC 1 cut(s) 2889
BstKTI GATC 9 cut(s) 76, 327, 688, 1273, 1294, 1381, 2239, 2441, 3208
BstMAI GTCTC 4 cut(s) 752, 1970, 2284, 3281
BstMBI GATC 9 cut(s) 73, 324, 685, 1270, 1291, 1378, 2236, 2438, 3205
BstMWI GCNNNNNNNGC 5 cut(s) 921, 2028, 2048, 2549, 2705
BstNI CCWGG 5 cut(s) 1053, 1538, 1719, 2696, 3002
BstNSI RCATGY 3 cut(s) 742, 1128, 1871
BstSFI CTRYAG 5 cut(s) 981, 2274, 2988, 3127, 3198
BstSLI GKGCMC 2 cut(s) 2044, 2970
BstUI CGCG 1 cut(s) 2858
BstV1I GCAGC 6 cut(s) 112, 967, 1172, 1903, 2720, 3113
BstV2I GAAGAC 5 cut(s) 1076, 1690, 2685, 3228, 3378
BstX2I RGATCY 1 cut(s) 324
BstXI CCANNNNNNTGG 1 cut(s) 3171
BstYI RGATCY 1 cut(s) 324
Bsu36I CCTNAGG 1 cut(s) 1383
BsuI GTATCC 1 cut(s) 3145
BsuRI GGCC 5 cut(s) 622, 940, 1888, 2693, 3175
BtgI CCRYGG 1 cut(s) 2010
BtgZI GCGATG 1 cut(s) 559
BtsCI GGATG 9 cut(s) 58, 184, 250, 289, 2078, 2080, 2082, 2888, 3018
BtsIMutI CAGTG 5 cut(s) 2793, 2832, 3114, 3183, 3480
BveI ACCTGC 1 cut(s) 1194
Cac8I GCNNGC 5 cut(s) 922, 1828, 2029, 2296, 3131
CaiI CAGNNNCTG 2 cut(s) 524, 1543
CciI TCATGA 1 cut(s) 3208
CfoI GCGC 1 cut(s) 2889
Cfr10I RCCGGY 4 cut(s) 18, 162, 228, 2532
Cfr13I GGNCC 6 cut(s) 7, 40, 150, 1046, 2135, 2692
CseI GACGC 2 cut(s) 2583, 3068
Csp6I GTAC 9 cut(s) 1056, 1325, 1402, 1567, 1761, 1842, 2069, 2852, 2920
CspCI CAANNNNNGTGG 2 cut(s) 1123, 1158
CviAII CATG 9 cut(s) 395, 739, 1125, 1448, 1615, 1868, 1973, 2011, 3209
CviQI GTAC 9 cut(s) 1056, 1325, 1402, 1567, 1761, 1842, 2069, 2852, 2920
DdeI CTNAG 6 cut(s) 87, 823, 1383, 1920, 2390, 3446
DinI GGCGCC 1 cut(s) 2888
DpnI GATC 9 cut(s) 75, 326, 687, 1272, 1293, 1380, 2238, 2440, 3207
DpnII GATC 9 cut(s) 73, 324, 685, 1270, 1291, 1378, 2236, 2438, 3205
DriI GACNNNNNGTC 1 cut(s) 1077
EaeI YGGCCR 1 cut(s) 3173
Eam1105I GACNNNNNGTC 1 cut(s) 1077
EciI GGCGGA 1 cut(s) 1958
Eco130I CCWWGG 3 cut(s) 626, 2010, 2455
Eco147I AGGCCT 1 cut(s) 622
Eco31I GGTCTC 1 cut(s) 1970
Eco32I GATATC 1 cut(s) 571
Eco47I GGWCC 5 cut(s) 7, 40, 150, 1046, 2135
Eco57I CTGAAG 4 cut(s) 1565, 1793, 2828, 3390
Eco72I CACGTG 2 cut(s) 2561, 3489
Eco81I CCTNAGG 1 cut(s) 1383
Eco88I CYCGRG 1 cut(s) 1193
EcoO109I RGGNCCY 1 cut(s) 1046
EcoRI GAATTC 1 cut(s) 1024
EcoRII CCWGG 5 cut(s) 1051, 1536, 1717, 2694, 3000
EcoRV GATATC 1 cut(s) 571
EcoT14I CCWWGG 3 cut(s) 626, 2010, 2455
EcoT22I ATGCAT 5 cut(s) 605, 744, 1801, 2080, 3143
EgeI GGCGCC 1 cut(s) 2888
EheI GGCGCC 1 cut(s) 2888
ErhI CCWWGG 3 cut(s) 626, 2010, 2455
FaeI CATG 9 cut(s) 398, 742, 1128, 1451, 1618, 1871, 1976, 2014, 3212
FalI AAGNNNNNCTT 4 cut(s) 1682, 1714, 2774, 2806
FaqI GGGAC 2 cut(s) 13, 2941
FatI CATG 9 cut(s) 394, 738, 1124, 1447, 1614, 1867, 1972, 2010, 3208
FauI CCCGC 1 cut(s) 133
FauNDI CATATG 2 cut(s) 1498, 3280
FbaI TGATCA 3 cut(s) 1291, 2236, 3205
Fnu4HI GCNGC 6 cut(s) 126, 981, 1161, 1917, 2709, 3127
FokI GGATG 9 cut(s) 65, 191, 257, 296, 2065, 2069, 2087, 2895, 3005
Fsp4HI GCNGC 6 cut(s) 126, 981, 1161, 1917, 2709, 3127
FspBI CTAG 5 cut(s) 1151, 1214, 1817, 2633, 3071
GlaI GCGC 1 cut(s) 2888
GluI GCNGC 6 cut(s) 126, 981, 1161, 1917, 2709, 3127
GsuI CTGGAG 1 cut(s) 2984
HaeII RGCGCY 1 cut(s) 2890
HaeIII GGCC 5 cut(s) 622, 940, 1888, 2693, 3175
HgaI GACGC 2 cut(s) 2583, 3068
HhaI GCGC 1 cut(s) 2889
Hin1I GRCGYC 1 cut(s) 2887
Hin1II CATG 9 cut(s) 398, 742, 1128, 1451, 1618, 1871, 1976, 2014, 3212
Hin6I GCGC 1 cut(s) 2887
HinP1I GCGC 1 cut(s) 2887
HincII GTYRAC 3 cut(s) 1183, 2598, 3334
HindII GTYRAC 3 cut(s) 1183, 2598, 3334
HindIII AAGCTT 3 cut(s) 89, 2720, 2870
HpaI GTTAAC 1 cut(s) 1183
HphI GGTGA 6 cut(s) 86, 494, 1010, 1618, 2343, 2474
Hpy166II GTNNAC 6 cut(s) 1183, 1569, 1761, 2598, 2920, 3334
Hpy8I GTNNAC 6 cut(s) 1183, 1569, 1761, 2598, 2920, 3334
Hpy99I CGWCG 1 cut(s) 244
HpyAV CCTTC 9 cut(s) 384, 805, 1540, 1603, 1676, 2212, 2776, 3342, 3376
HpyCH4III ACNGT 9 cut(s) 995, 1669, 2491, 2660, 2836, 2943, 2989, 3109, 3157
HpyCH4IV ACGT 3 cut(s) 2560, 3330, 3488
HpyF10VI GCNNNNNNNGC 5 cut(s) 921, 2028, 2048, 2549, 2705
HpyF3I CTNAG 6 cut(s) 87, 823, 1383, 1920, 2390, 3446
HpySE526I ACGT 3 cut(s) 2560, 3330, 3488
Hsp92I GRCGYC 1 cut(s) 2887
Hsp92II CATG 9 cut(s) 398, 742, 1128, 1451, 1618, 1871, 1976, 2014, 3212
HspAI GCGC 1 cut(s) 2887
KasI GGCGCC 1 cut(s) 2886
Kpn2I TCCGGA 1 cut(s) 1170
KpnI GGTACC 1 cut(s) 1328
Ksp22I TGATCA 3 cut(s) 1291, 2236, 3205
KspAI GTTAAC 1 cut(s) 1183
Kzo9I GATC 9 cut(s) 73, 324, 685, 1270, 1291, 1378, 2236, 2438, 3205
LmnI GCTCC 6 cut(s) 138, 196, 204, 262, 1135, 1540
Lsp1109I GCAGC 6 cut(s) 112, 967, 1172, 1903, 2720, 3113
LweI GCATC 6 cut(s) 46, 1856, 2065, 2087, 2632, 3479
MaeI CTAG 5 cut(s) 1151, 1214, 1817, 2633, 3071
MaeII ACGT 3 cut(s) 2560, 3330, 3488
MaeIII GTNAC 8 cut(s) 340, 353, 426, 482, 499, 1188, 1235, 2612
MalI GATC 9 cut(s) 75, 326, 687, 1272, 1293, 1380, 2238, 2440, 3207
MboI GATC 9 cut(s) 73, 324, 685, 1270, 1291, 1378, 2236, 2438, 3205
MflI RGATCY 1 cut(s) 324
MhlI GDGCHC 4 cut(s) 26, 1132, 2044, 2970
MlsI TGGCCA 1 cut(s) 3175
MluNI TGGCCA 1 cut(s) 3175
Mly113I GGCGCC 1 cut(s) 2887
MlyI GAGTC 6 cut(s) 224, 1000, 1087, 1161, 2609, 2747
MmeI TCCRAC 3 cut(s) 156, 222, 544
Mox20I TGGCCA 1 cut(s) 3175
Mph1103I ATGCAT 5 cut(s) 605, 744, 1801, 2080, 3143
MroI TCCGGA 1 cut(s) 1170
MroXI GAANNNNTTC 2 cut(s) 365, 675
MscI TGGCCA 1 cut(s) 3175
MslI CAYNNNNRTG 1 cut(s) 338
Msp20I TGGCCA 1 cut(s) 3175
MspA1I CMGCKG 2 cut(s) 3126, 3203
Mva1269I GAATGC 3 cut(s) 1441, 2596, 3013
MvaI CCWGG 5 cut(s) 1053, 1538, 1719, 2696, 3002
MvnI CGCG 1 cut(s) 2858
MwoI GCNNNNNNNGC 5 cut(s) 921, 2028, 2048, 2549, 2705
NarI GGCGCC 1 cut(s) 2887
NciI CCSGG 6 cut(s) 119, 154, 185, 220, 251, 480
NcoI CCATGG 1 cut(s) 2010
NdeI CATATG 2 cut(s) 1498, 3280
NdeII GATC 9 cut(s) 73, 324, 685, 1270, 1291, 1378, 2236, 2438, 3205
NlaIII CATG 9 cut(s) 398, 742, 1128, 1451, 1618, 1871, 1976, 2014, 3212
NlaIV GGNNCC 6 cut(s) 151, 326, 1047, 1326, 2453, 2888
NmuCI GTSAC 5 cut(s) 340, 426, 482, 1188, 2612
NsiI ATGCAT 5 cut(s) 605, 744, 1801, 2080, 3143
NspI RCATGY 3 cut(s) 742, 1128, 1871
NspV TTCGAA 1 cut(s) 3320
OliI CACNNNNGTG 1 cut(s) 338
PaeR7I CTCGAG 1 cut(s) 1193
PagI TCATGA 1 cut(s) 3208
PceI AGGCCT 1 cut(s) 622
PciI ACATGT 1 cut(s) 1124
PcsI WCGNNNNNNNCGW 2 cut(s) 69, 210
PctI GAATGC 3 cut(s) 1441, 2596, 3013
PdmI GAANNNNTTC 2 cut(s) 365, 675
PflFI GACNNNGTC 1 cut(s) 1616
PflMI CCANNNNNTGG 1 cut(s) 563
PfoI TCCNGGA 5 cut(s) 117, 183, 249, 1717, 3000
PkrI GCNGC 6 cut(s) 127, 982, 1162, 1918, 2710, 3128
PleI GAGTC 6 cut(s) 223, 1000, 1086, 1161, 2609, 2747
PluTI GGCGCC 1 cut(s) 2890
PmaCI CACGTG 2 cut(s) 2561, 3489
PmlI CACGTG 2 cut(s) 2561, 3489
PpsI GAGTC 6 cut(s) 223, 1000, 1086, 1161, 2609, 2747
Ppu21I YACGTR 2 cut(s) 2561, 3489
PpuMI RGGWCCY 1 cut(s) 1046
PscI ACATGT 1 cut(s) 1124
PshBI ATTAAT 2 cut(s) 1037, 1802
PsiI TTATAA 2 cut(s) 2304, 2667
Psp5II RGGWCCY 1 cut(s) 1046
Psp6I CCWGG 5 cut(s) 1051, 1536, 1717, 2694, 3000
PspCI CACGTG 2 cut(s) 2561, 3489
PspGI CCWGG 5 cut(s) 1051, 1536, 1717, 2694, 3000
PspN4I GGNNCC 6 cut(s) 151, 326, 1047, 1326, 2453, 2888
PspPI GGNCC 6 cut(s) 7, 40, 150, 1046, 2135, 2692
PspPPI RGGWCCY 1 cut(s) 1046
PspXI VCTCGAGB 1 cut(s) 1193
PsrI GAACNNNNNNTAC 2 cut(s) 1744, 1776
PstI CTGCAG 2 cut(s) 985, 3131
PstNI CAGNNNCTG 2 cut(s) 524, 1543
PsuI RGATCY 1 cut(s) 324
PsyI GACNNNGTC 1 cut(s) 1616
PvuII CAGCTG 1 cut(s) 3203
RsaI GTAC 9 cut(s) 1057, 1326, 1403, 1568, 1762, 1843, 2070, 2853, 2921
RsaNI GTAC 9 cut(s) 1056, 1325, 1402, 1567, 1761, 1842, 2069, 2852, 2920
RseI CAYNNNNRTG 1 cut(s) 338
SatI GCNGC 6 cut(s) 126, 981, 1161, 1917, 2709, 3127
Sau3AI GATC 9 cut(s) 73, 324, 685, 1270, 1291, 1378, 2236, 2438, 3205
Sau96I GGNCC 6 cut(s) 7, 40, 150, 1046, 2135, 2692
ScaI AGTACT 1 cut(s) 2853
SchI GAGTC 6 cut(s) 224, 1000, 1087, 1161, 2609, 2747
SduI GDGCHC 4 cut(s) 26, 1132, 2044, 2970
SfaNI GCATC 6 cut(s) 46, 1856, 2065, 2087, 2632, 3479
SfcI CTRYAG 5 cut(s) 981, 2274, 2988, 3127, 3198
SfoI GGCGCC 1 cut(s) 2888
Sfr274I CTCGAG 1 cut(s) 1193
SfuI TTCGAA 1 cut(s) 3320
SgrAI CRCCGGYG 1 cut(s) 18
SinI GGWCC 5 cut(s) 7, 40, 150, 1046, 2135
SlaI CTCGAG 1 cut(s) 1193
SmiMI CAYNNNNRTG 1 cut(s) 338
SmlI CTYRAG 5 cut(s) 1193, 1637, 2174, 2364, 2717
SmoI CTYRAG 5 cut(s) 1193, 1637, 2174, 2364, 2717
SseBI AGGCCT 1 cut(s) 622
SsiI CCGC 5 cut(s) 16, 140, 1145, 1969, 3124
SspDI GGCGCC 1 cut(s) 2886
SspI AATATT 3 cut(s) 1141, 1394, 1836
SspMI CTAG 5 cut(s) 1151, 1214, 1817, 2633, 3071
StuI AGGCCT 1 cut(s) 622
StyI CCWWGG 3 cut(s) 626, 2010, 2455
TaaI ACNGT 9 cut(s) 995, 1669, 2491, 2660, 2836, 2943, 2989, 3109, 3157
TaiI ACGT 3 cut(s) 2563, 3333, 3491
TatI WGTACW 6 cut(s) 1401, 1566, 1760, 1841, 2068, 2851
TscAI CASTG 5 cut(s) 2800, 2839, 3114, 3183, 3487
TseFI GTSAC 5 cut(s) 340, 426, 482, 1188, 2612
TseI GCWGC 6 cut(s) 125, 980, 1160, 1916, 2708, 3126
Tsp45I GTSAC 5 cut(s) 340, 426, 482, 1188, 2612
TspGWI ACGGA 3 cut(s) 791, 2668, 3305
TspRI CASTG 5 cut(s) 2800, 2839, 3114, 3183, 3487
Tth111I GACNNNGTC 1 cut(s) 1616
Van91I CCANNNNNTGG 1 cut(s) 563
VpaK11BI GGWCC 5 cut(s) 7, 40, 150, 1046, 2135
VspI ATTAAT 2 cut(s) 1037, 1802
XceI RCATGY 3 cut(s) 742, 1128, 1871
XcmI CCANNNNNNNNNTGG 1 cut(s) 2129
XhoI CTCGAG 1 cut(s) 1193
XmnI GAANNNNTTC 2 cut(s) 365, 675
XspI CTAG 5 cut(s) 1151, 1214, 1817, 2633, 3071
ZrmI AGTACT 1 cut(s) 2853
Zsp2I ATGCAT 5 cut(s) 605, 744, 1801, 2080, 3143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.