MD14G1146400.v1.1

AAA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000279
Physical Location & Seq
Forward (+)
23916381 .. 23919794
3414 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1146400.v1.1.491

Sequence Viewer

Length: 2628 bp
ATGGATCGTCAGGAGAGTAGTAAGTTGAACAATTATGGAGTTGCAGCAGCTCGACCCTTAATCAATATCGTCTTCTCTTGGTCACTTGAGGATGTTCTCAATGCACATCTTTACCGAAATCAGGTGACAAAGATTCCTGATACATTTTCTACCGTGACGAGTTACATGAAATCTTTCATTCCTTCACTTGTTGAGGAAACACATGCTGATCTACTCTCAAGCATGATGAATCTGTCGCAGGCCCCTACTCTCGAAATTCTGACTGTTAAAACTTCTAAGAATCATGGACCTCCTAAAGACTTGTTTTATGATGTTACTTGTGTAGGAACATATGTGCCACAGGTTGGAGATCTTATTGCCTTAACTGATATTAAACCAAAATGCGTTGATCATTTGAACGGGCCCAGAATTTCCTATCTCATTGCATATGTTCATCAAAGTAGAGACAGTAATCTCTCAATACTCTCGTCAAAGCATATCGATACAGGAAGATACACGCATATTAAGAGCAAGACAGATACACTTTTTGCTGTCTATCTACTGAACATGACAACAACTGTTCGCGTATGGAAAGCTTTGAACTCAAAAGGGGCAAATACAAGTTTAACTAAAAACTTGCTGCAACCGCAAGCCAATTCATCACAAGGTAGGAATTCGTGTTCAATTTGCTTTGACAACGAAAAATGCTACCTATCTGCTACATTTCATGCAATGTGCTCTGATCTAAATGATTCCCAAAAGGCTGCAGTTTTGAATTGTATTAGTTTAAGTAAATGCAACCACCGTAATACCATAAAACTAATATGGGGACCTCCTGGAACTGGAAAAACGAAGACAGTCGGTATGTCACTCTTTGCCATGGTTAAATTGAAGTGCAGAACATTAACATGTGCTCCAACCAATATTGCCGTGTTAGAAGTAACAGCACGCGTGCTGAGATTGGTAAATCAGTCACTTGATTACGGTAAGTACGGGCTTGGGGATATAATTCTATTTGGGAACGAGCAGAGGATGAAGATTGATGATCATGATGACCTTGTTGATGTATTTCTTGATTATCGTACTAAAATTCTGGCCGAGTGTTTTGCCCCTTTGAGTGGATGGAAACATCGGTTAGCATCAATGATTGATTTACTTGAGGATCCAAAGGAAAAGTACGCAGCGTATTTGAGAGAAAAAAGGAGAAGAAACAATGAGGATAAAGAGGGCGATAATACATGGATGAAGATGCTGAAGGGAGTGATAATGTCGTTAATCAATGGTAGAAACTCAACAAGTGACGATGATAATGATCTTATGACATTTGAGGAGTTTGTGAAGGAAGAATTTGATTCCATTGGTGAGTGCCTCAAGATTTGTATGGTAAATTTGTACACCCACTTACCAACTTCTTGCATGTCCATAAAGGTAGTCAAGGACATGATTACAGCTTTGGATTTGCTCAACTCTTGCAAATATTTATTTGATGAGGTTGGTTTTTCTAATGAAAGGATGCAATTAGTTTGTATACTGAGGTCGCTTCGCGCATTTTCTGTTCCTATTTTGACTGACGAGAAGGAAATAGGTAATTTTTGCTTGGAAAATGCTTGCTTAATATGTTGCACTGTCTCGAGCTCTTCTAAATTGTACACAAAAGGAATGAAACCGGTAGAAATATTGATGGTTGATGAAGCTGCTCAGCTTAAAGAATGTGAATCGTTAATTCCTTTGCAACTCCCTGGTCTTCGCCATGCTATTCTCATTGGGGATCAAAGGCAACTCCCCGCTATGGTGAAAAGCAAGATCTCCGAGAAGGCTGTATTTGGGAGAAGTTTGTTTGAAAGACTTGTACAGTTGGGACACGAGAAGCTCCTTCTCAATGTCCAGTATAGAATGCATCCTTGGATCAGTTTATTTCCTAATAGAGAGTTTTACAACCACCGGTTACTAGATGGTTCGAATGTCAATAAAAAAAGCTATGACAAGTGCTTTCTTTCGGGAAAAATGTACGGATGCTACTCGTTTATTAATATAGCCAATGGAAAAGAAGAATTTGATCGCGGGAGTAGCCAGAAAAATATGGCTGAGGTTGCTGTGGTCTATGAGATAGTTGCAAGCCTATACAGAGAGTTCACTTGCACAAAGAAGAAGGTTAGTGTCGGAGTAATATCACCTTACAAAGCACAGGTTGATGCAATTCAAGCGAGGGTCAGAAAATACAGTGCAAACTCTGGAACTAGTGACTTTGCCCTAAGTGTGCGATCTGTTGACGGATTCCAAGGTGGCGAAGAGGATGTGATAATAATCTCCACAGTCAGATGTAATGAGAATGGATCTGTGGGATTCCTCTCGAATCGTCAGAGAGCGAATGTGATGCTAACACGTGCAAGGTATTGTCTTTGGATATTGGGGAACGAACCAACTTTGGTTAAGAGTGGGAGTATTTGGAAGAAGGTAATTGTTGATGCTAAGAAACGCAAATGTTTCTATAATGCTGAGGAGGACAAGGACTTGTCTCAGCCTTTGGCACCTGCCCTTGTGGATTCGAGCAGTTCCCGGGAAGTTGATCCCGCGGAGTTCCTTTCAAAACCGTTATCTTCTCTCAGTCTAACAGGAGAGCCACAGACACCATCAAGTAGTTATAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12

Protein Analysis

876

Amino Acids

98.23

Weight (kDa)

8.59

Isoelectric Point (pI)

39.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 113 - 197 3.9e-07 Domain of unknown function (DUF6469)
AAA_11 PF13086 242 - 594 1.9e-34 AAA domain
AAA_12 PF13087 602 - 799 7.9e-56 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000279)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65810 AT1G65810 AT1G65810 AT4G05540 AT5G37140 AT5G37150 AT5G37160 AT5G37165 AT5G52090
fragaria_vesca FvH4_1g27541 FvH4_1g27542 FvH4_2g08960 FvH4_2g08960 FvH4_2g08960 FvH4_2g08960 FvH4_3g00170 FvH4_3g00170 FvH4_3g00170 FvH4_5g05930 FvH4_5g05930
malus_domestica MD08G1077700.v1.1 MD08G1077900.v1.1 MD08G1078000.v1.1 MD08G1078100.v1.1 MD08G1078300.v1.1 MD09G1057800.v1.1 MD09G1281400.v1.1 MD11G1222900.v1.1 MD14G1146400.v1.1
prunus_persica Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G202100_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.3G013100_v2.0.a1
pyrus_communis pycom09g18900 pycom09g18910 pycom13g26720 pycom13g26730 pycom13g26760 pycom13g26770
rosa_chinensis RchiOBHm_Chr3g0488001 RchiOBHm_Chr3g0488011 RchiOBHm_Chr3g0488031 RchiOBHm_Chr3g0488061 RchiOBHm_Chr3g0488111 RchiOBHm_Chr3g0488201 RchiOBHm_Chr3g0488211 RchiOBHm_Chr3g0488221 RchiOBHm_Chr3g0488321 RchiOBHm_Chr3g0488431 RchiOBHm_Chr6g0264601 RchiOBHm_Chr6g0264641
rosa_laevigata RLG00000014240 RLG00000014241 RLG00000022910 RLG00000022949 RLG00000030376
rosa_multiflora Rmu_co8075388.1_g000001 Rmu_co8424869.1_g000001 Rmu_co8427783.1_g000001 Rmu_sc0000546.1_g000002 Rmu_sc0000546.1_g000015 Rmu_sc0011052.1_g000014 Rmu_sc0017772.1_g000004
rosa_roxburghii Rroxscaffold_175G00431980 Rroxscaffold_175G00431990 Rroxscaffold_1G00034600 Rroxscaffold_6G00394230 Rroxscaffold_6G00394270 Rroxscaffold_6G00394280 Rroxscaffold_6G00394350 Rroxscaffold_7G00203240 Rroxscaffold_7G00203270 Rroxscaffold_7G00203280 Rroxscaffold_7G00203300 Rroxscaffold_7G00205740
rosa_rugosa Rorug03G0238000 Rorug03G0238100 Rorug03G0238100 Rorug03G0238500 Rorug05G0155400 Rorug05G0356000 Rorug05G0463000 Rorug06G0015200 Rorug06G0015300 Rorug06G0015400 Rorug06G0015500 Rorug06G0015500
rosa_samantha Rh1DG088100 Rh2AG244800 Rh2BG187500 Rh2CG247300 Rh3BG322700 Rh3BG322800 Rh3BG323100 Rh3BG323900 Rh3BG324000 Rh3BG324300 Rh3CG321300 Rh3CG321400 Rh3CG321500 Rh3DG319400 Rh3DG319900 Rh3DG320100 Rh3DG320200 Rh6AG133700 Rh6AG133800 Rh6AG133900 Rh6CG129300 Rh6CG129800 Rh6CG130200 Rh6DG116500 Rh6DG117200 Rh7DG378000
rosa_wichuraiana Rw2G014710 Rw3G025340 Rw3G025350 Rw3G025360 Rw5G020630 Rw6G011590 Rw6G011600 Rw7G041380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2518
Acc36I ACCTGC 1 cut(s) 2518
AccB1I GGYRCC 1 cut(s) 2506
AccB7I CCANNNNNTGG 1 cut(s) 344
AccI GTMKAC 1 cut(s) 1507
AccII CGCG 5 cut(s) 564, 930, 1524, 2040, 2552
AciI CCGC 5 cut(s) 626, 1764, 2040, 2550, 2552
AclWI GGATC 7 cut(s) 12, 1136, 1149, 1755, 1892, 2320, 2540
AcoI YGGCCR 1 cut(s) 1074
AcsI RAATTY 7 cut(s) 255, 408, 652, 1068, 1325, 1366, 2030
AcuI CTGAAG 1 cut(s) 1253
AcvI CACGTG 1 cut(s) 2363
AfaI GTAC 7 cut(s) 971, 1063, 1157, 1373, 1628, 1830, 1988
AfiI CCNNNNNNNGG 5 cut(s) 121, 344, 821, 1097, 1768
AflIII ACRYGT 3 cut(s) 887, 928, 2360
AgeI ACCGGT 2 cut(s) 1645, 1920
AgsI TTSAA 9 cut(s) 28, 397, 580, 663, 754, 871, 1820, 2180, 2565
AhlI ACTAGT 1 cut(s) 2216
AjnI CCWGG 2 cut(s) 814, 1717
AjuI GAANNNNNNNTTGG 2 cut(s) 1864, 1896
AloI GAACNNNNNNTCC 2 cut(s) 643, 675
AluBI AGCT 8 cut(s) 50, 575, 1430, 1614, 1673, 1681, 1849, 1956
AluI AGCT 8 cut(s) 50, 575, 1430, 1614, 1673, 1681, 1849, 1956
Alw21I GWGCWC 3 cut(s) 719, 895, 1616
Alw26I GTCTC 3 cut(s) 438, 1612, 2499
AlwI GGATC 7 cut(s) 12, 1136, 1149, 1755, 1892, 2320, 2540
Ama87I CYCGRG 2 cut(s) 1609, 2535
AoxI GGCC 3 cut(s) 240, 401, 1074
ApaI GGGCCC 1 cut(s) 405
ApeKI GCWGC 6 cut(s) 44, 47, 619, 743, 1160, 1673
ApoI RAATTY 7 cut(s) 255, 408, 652, 1068, 1325, 1366, 2030
AseI ATTAAT 1 cut(s) 2007
AsiGI ACCGGT 2 cut(s) 1645, 1920
Asp700I GAANNNNTTC 1 cut(s) 173
AspLEI GCGC 1 cut(s) 1526
AspS9I GGNCC 5 cut(s) 241, 287, 401, 402, 809
AsuC2I CCSGG 2 cut(s) 2536, 2537
AsuHPI GGTGA 4 cut(s) 136, 1352, 1783, 2142
AsuII TTCGAA 1 cut(s) 1937
AvaI CYCGRG 2 cut(s) 1609, 2535
AvaII GGWCC 2 cut(s) 287, 809
BaeGI GKGCMC 1 cut(s) 405
BamHI GGATCC 1 cut(s) 1141
BanI GGYRCC 1 cut(s) 2506
BanII GRGCYC 2 cut(s) 405, 1616
BauI CACGAG 1 cut(s) 1841
BbrPI CACGTG 1 cut(s) 2363
BbsI GAAGAC 3 cut(s) 64, 839, 1715
Bbv12I GWGCWC 3 cut(s) 719, 895, 1616
BbvCI CCTCAGC 2 cut(s) 2064, 2475
BbvI GCAGC 6 cut(s) 56, 59, 606, 730, 1172, 1660
BccI CCATC 4 cut(s) 1095, 1654, 1925, 2617
BceAI ACGGC 1 cut(s) 893
BcgI CGANNNNNNTGC 4 cut(s) 1067, 1101, 2335, 2369
BciT130I CCWGG 2 cut(s) 816, 1719
BclI TGATCA 2 cut(s) 388, 1024
BcnI CCSGG 2 cut(s) 2536, 2537
BcoDI GTCTC 3 cut(s) 438, 1612, 2499
BcuI ACTAGT 1 cut(s) 2216
BfaI CTAG 2 cut(s) 1928, 2217
BfmI CTRYAG 1 cut(s) 744
BfuAI ACCTGC 1 cut(s) 2518
BglII AGATCT 2 cut(s) 349, 1782
BisI GCNGC 6 cut(s) 45, 48, 620, 744, 1161, 1674
BlpI GCTNAGC 1 cut(s) 1677
BlsI GCNGC 6 cut(s) 46, 49, 621, 745, 1162, 1675
Bme1390I CCNGG 4 cut(s) 816, 1719, 2536, 2537
Bme18I GGWCC 2 cut(s) 287, 809
BmeT110I CYCGRG 2 cut(s) 1609, 2535
BmgT120I GGNCC 5 cut(s) 241, 287, 401, 402, 809
BmiI GGNNCC 5 cut(s) 243, 403, 810, 1143, 2508
BmrFI CCNGG 4 cut(s) 816, 1719, 2536, 2537
BmsI GCATC 8 cut(s) 1127, 1218, 1482, 1885, 1982, 2161, 2343, 2434
BpiI GAAGAC 3 cut(s) 64, 839, 1715
Bpu10I CCTNAGC 2 cut(s) 2064, 2475
Bpu1102I GCTNAGC 1 cut(s) 1677
Bpu14I TTCGAA 1 cut(s) 1937
BpuEI CTTGAG 4 cut(s) 107, 202, 1157, 1334
BpuMI CCSGG 2 cut(s) 2536, 2537
Bsa29I ATCGAT 1 cut(s) 480
BsaAI YACGTR 1 cut(s) 2363
BsaBI GATNNNNATC 2 cut(s) 1290, 2282
BsaJI CCNNGG 6 cut(s) 858, 1717, 1880, 2257, 2535, 2550
BsaWI WCCGGW 2 cut(s) 1645, 1920
BsaXI ACNNNNNCTCC 2 cut(s) 877, 907
Bsc4I CCNNNNNNNGG 5 cut(s) 121, 344, 821, 1097, 1768
Bse118I RCCGGY 2 cut(s) 1645, 1920
Bse1I ACTGG 2 cut(s) 826, 1864
Bse3DI GCAATG 2 cut(s) 420, 717
Bse8I GATNNNNATC 2 cut(s) 1290, 2282
BseBI CCWGG 2 cut(s) 816, 1719
BseCI ATCGAT 1 cut(s) 480
BseDI CCNNGG 6 cut(s) 858, 1717, 1880, 2257, 2535, 2550
BseGI GGATG 8 cut(s) 97, 1017, 1106, 1227, 1497, 1876, 1997, 2278
BseJI GATNNNNATC 2 cut(s) 1290, 2282
BseLI CCNNNNNNNGG 5 cut(s) 121, 344, 821, 1097, 1768
BseMI GCAATG 2 cut(s) 420, 717
BseMII CTCAG 7 cut(s) 926, 1502, 1691, 2055, 2466, 2510, 2596
BseNI ACTGG 2 cut(s) 826, 1864
BseRI GAGGAG 2 cut(s) 1322, 2492
BseSI GKGCMC 1 cut(s) 405
BseXI GCAGC 6 cut(s) 56, 59, 606, 730, 1172, 1660
BsgI GTGCAG 1 cut(s) 895
Bsh1236I CGCG 5 cut(s) 564, 930, 1524, 2040, 2552
BshFI GGCC 3 cut(s) 242, 403, 1076
BshNI GGYRCC 1 cut(s) 2506
BshTI ACCGGT 2 cut(s) 1645, 1920
BshVI ATCGAT 1 cut(s) 480
BsiHKAI GWGCWC 3 cut(s) 719, 895, 1616
BsiHKCI CYCGRG 2 cut(s) 1609, 2535
BsiSI CCGG 3 cut(s) 1646, 1921, 2536
BslFI GGGAC 2 cut(s) 822, 1851
BslI CCNNNNNNNGG 5 cut(s) 121, 344, 821, 1097, 1768
BsmAI GTCTC 3 cut(s) 438, 1612, 2499
BsmFI GGGAC 2 cut(s) 822, 1851
BsmI GAATGC 1 cut(s) 1878
BsnI GGCC 3 cut(s) 242, 403, 1076
BsoBI CYCGRG 2 cut(s) 1609, 2535
Bsp119I TTCGAA 1 cut(s) 1937
Bsp120I GGGCCC 1 cut(s) 401
Bsp1286I GDGCHC 4 cut(s) 405, 719, 895, 1616
Bsp1407I TGTACA 3 cut(s) 1371, 1626, 1828
Bsp1720I GCTNAGC 1 cut(s) 1677
Bsp19I CCATGG 1 cut(s) 858
BspACI CCGC 5 cut(s) 626, 1764, 2040, 2550, 2552
BspANI GGCC 3 cut(s) 242, 403, 1076
BspCNI CTCAG 7 cut(s) 927, 1503, 1690, 2056, 2467, 2509, 2595
BspDI ATCGAT 1 cut(s) 480
BspFNI CGCG 5 cut(s) 564, 930, 1524, 2040, 2552
BspHI TCATGA 1 cut(s) 1027
BspLI GGNNCC 5 cut(s) 243, 403, 810, 1143, 2508
BspMAI CTGCAG 1 cut(s) 748
BspMI ACCTGC 1 cut(s) 2518
BspPI GGATC 7 cut(s) 12, 1136, 1149, 1755, 1892, 2320, 2540
BspQI GCTCTTC 1 cut(s) 1621
BspT104I TTCGAA 1 cut(s) 1937
BspT107I GGYRCC 1 cut(s) 2506
BsrDI GCAATG 2 cut(s) 420, 717
BsrFI RCCGGY 2 cut(s) 1645, 1920
BsrGI TGTACA 3 cut(s) 1371, 1626, 1828
BsrI ACTGG 2 cut(s) 826, 1864
BssAI RCCGGY 2 cut(s) 1645, 1920
BssECI CCNNGG 6 cut(s) 858, 1717, 1880, 2257, 2535, 2550
BssNAI GTATAC 1 cut(s) 1508
BssSI CACGAG 1 cut(s) 1841
BssT1I CCWWGG 3 cut(s) 858, 1880, 2257
Bst1107I GTATAC 1 cut(s) 1508
Bst2BI CACGAG 1 cut(s) 1841
Bst2UI CCWGG 2 cut(s) 816, 1719
Bst6I CTCTTC 2 cut(s) 1621, 2262
BstAUI TGTACA 3 cut(s) 1371, 1626, 1828
BstBAI YACGTR 1 cut(s) 2363
BstBI TTCGAA 1 cut(s) 1937
BstC8I GCNNGC 6 cut(s) 240, 630, 928, 932, 1588, 2095
BstDSI CCRYGG 2 cut(s) 858, 2550
BstF5I GGATG 8 cut(s) 97, 1017, 1106, 1227, 1497, 1876, 1997, 2278
BstFNI CGCG 5 cut(s) 564, 930, 1524, 2040, 2552
BstHHI GCGC 1 cut(s) 1526
BstMAI GTCTC 3 cut(s) 438, 1612, 2499
BstMWI GCNNNNNNNGC 4 cut(s) 625, 2046, 2069, 2180
BstNI CCWGG 2 cut(s) 816, 1719
BstNSI RCATGY 3 cut(s) 206, 891, 1399
BstSCI CCNGG 4 cut(s) 814, 1717, 2534, 2535
BstSFI CTRYAG 1 cut(s) 744
BstSLI GKGCMC 1 cut(s) 405
BstUI CGCG 5 cut(s) 564, 930, 1524, 2040, 2552
BstV1I GCAGC 6 cut(s) 56, 59, 606, 730, 1172, 1660
BstV2I GAAGAC 3 cut(s) 64, 839, 1715
BstX2I RGATCY 4 cut(s) 349, 1141, 1782, 2312
BstYI RGATCY 4 cut(s) 349, 1141, 1782, 2312
BstZ17I GTATAC 1 cut(s) 1508
Bsu15I ATCGAT 1 cut(s) 480
BsuRI GGCC 3 cut(s) 242, 403, 1076
BsuTUI ATCGAT 1 cut(s) 480
BtgI CCRYGG 2 cut(s) 858, 2550
BtsCI GGATG 8 cut(s) 97, 1017, 1106, 1227, 1497, 1876, 1997, 2278
BtsIMutI CAGTG 2 cut(s) 1602, 2206
BveI ACCTGC 1 cut(s) 2518
Cac8I GCNNGC 6 cut(s) 240, 630, 928, 932, 1588, 2095
CciI TCATGA 1 cut(s) 1027
CfoI GCGC 1 cut(s) 1526
Cfr10I RCCGGY 2 cut(s) 1645, 1920
Cfr13I GGNCC 5 cut(s) 241, 287, 401, 402, 809
Cfr42I CCGCGG 1 cut(s) 2553
Cfr9I CCCGGG 1 cut(s) 2535
ClaI ATCGAT 1 cut(s) 480
Csp6I GTAC 7 cut(s) 970, 1062, 1156, 1372, 1627, 1829, 1987
CspAI ACCGGT 2 cut(s) 1645, 1920
CviQI GTAC 7 cut(s) 970, 1062, 1156, 1372, 1627, 1829, 1987
EaeI YGGCCR 1 cut(s) 1074
Eam1104I CTCTTC 2 cut(s) 1621, 2262
EarI CTCTTC 2 cut(s) 1621, 2262
Ecl136II GAGCTC 1 cut(s) 1614
Eco130I CCWWGG 3 cut(s) 858, 1880, 2257
Eco24I GRGCYC 2 cut(s) 405, 1616
Eco47I GGWCC 2 cut(s) 287, 809
Eco53kI GAGCTC 1 cut(s) 1614
Eco57I CTGAAG 1 cut(s) 1253
Eco72I CACGTG 1 cut(s) 2363
Eco88I CYCGRG 2 cut(s) 1609, 2535
EcoICRI GAGCTC 1 cut(s) 1614
EcoO109I RGGNCCY 2 cut(s) 241, 809
EcoRI GAATTC 1 cut(s) 652
EcoRII CCWGG 2 cut(s) 814, 1717
EcoT14I CCWWGG 3 cut(s) 858, 1880, 2257
EcoT22I ATGCAT 1 cut(s) 1878
EcoT38I GRGCYC 2 cut(s) 405, 1616
ErhI CCWWGG 3 cut(s) 858, 1880, 2257
FaqI GGGAC 2 cut(s) 822, 1851
FauI CCCGC 3 cut(s) 1771, 2033, 2557
FauNDI CATATG 2 cut(s) 331, 427
FbaI TGATCA 2 cut(s) 388, 1024
FblI GTMKAC 1 cut(s) 1507
Fnu4HI GCNGC 6 cut(s) 45, 48, 620, 744, 1161, 1674
FokI GGATG 8 cut(s) 104, 1024, 1113, 1234, 1504, 1863, 2004, 2285
FriOI GRGCYC 2 cut(s) 405, 1616
Fsp4HI GCNGC 6 cut(s) 45, 48, 620, 744, 1161, 1674
FspBI CTAG 2 cut(s) 1928, 2217
GlaI GCGC 1 cut(s) 1525
GluI GCNGC 6 cut(s) 45, 48, 620, 744, 1161, 1674
HaeIII GGCC 3 cut(s) 242, 403, 1076
HapII CCGG 3 cut(s) 1646, 1921, 2536
HhaI GCGC 1 cut(s) 1526
Hin6I GCGC 1 cut(s) 1524
HinP1I GCGC 1 cut(s) 1524
HincII GTYRAC 1 cut(s) 2248
HindII GTYRAC 1 cut(s) 2248
HindIII AAGCTT 1 cut(s) 573
HpaII CCGG 3 cut(s) 1646, 1921, 2536
HphI GGTGA 4 cut(s) 136, 1352, 1783, 2142
Hpy166II GTNNAC 5 cut(s) 1374, 1508, 1629, 2112, 2248
Hpy188I TCNGA 7 cut(s) 261, 721, 1789, 2141, 2192, 2297, 2340
Hpy8I GTNNAC 5 cut(s) 1374, 1508, 1629, 2112, 2248
HpyAV CCTTC 8 cut(s) 192, 1228, 1312, 1549, 1786, 1862, 2122, 2425
HpyCH4IV ACGT 1 cut(s) 2362
HpyF10VI GCNNNNNNNGC 4 cut(s) 625, 2046, 2069, 2180
HpySE526I ACGT 1 cut(s) 2362
HspAI GCGC 1 cut(s) 1524
Ksp22I TGATCA 2 cut(s) 388, 1024
KspI CCGCGG 1 cut(s) 2553
LguI GCTCTTC 1 cut(s) 1621
LmnI GCTCC 2 cut(s) 898, 1854
Lsp1109I GCAGC 6 cut(s) 56, 59, 606, 730, 1172, 1660
LweI GCATC 8 cut(s) 1127, 1218, 1482, 1885, 1982, 2161, 2343, 2434
MaeI CTAG 2 cut(s) 1928, 2217
MaeII ACGT 1 cut(s) 2362
MflI RGATCY 4 cut(s) 349, 1141, 1782, 2312
MhlI GDGCHC 4 cut(s) 405, 719, 895, 1616
MluI ACGCGT 1 cut(s) 928
MmeI TCCRAC 3 cut(s) 325, 920, 2119
Mph1103I ATGCAT 1 cut(s) 1878
MroXI GAANNNNTTC 1 cut(s) 173
MslI CAYNNNNRTG 1 cut(s) 886
MspA1I CMGCKG 1 cut(s) 2552
MspI CCGG 3 cut(s) 1646, 1921, 2536
MspR9I CCNGG 4 cut(s) 816, 1719, 2536, 2537
Mva1269I GAATGC 1 cut(s) 1878
MvaI CCWGG 2 cut(s) 816, 1719
MvnI CGCG 5 cut(s) 564, 930, 1524, 2040, 2552
MwoI GCNNNNNNNGC 4 cut(s) 625, 2046, 2069, 2180
NciI CCSGG 2 cut(s) 2536, 2537
NcoI CCATGG 1 cut(s) 858
NdeI CATATG 2 cut(s) 331, 427
NlaIV GGNNCC 5 cut(s) 243, 403, 810, 1143, 2508
NmeAIII GCCGAG 1 cut(s) 1102
NmuCI GTSAC 7 cut(s) 81, 124, 154, 846, 951, 1277, 2219
NsiI ATGCAT 1 cut(s) 1878
NspI RCATGY 3 cut(s) 206, 891, 1399
NspV TTCGAA 1 cut(s) 1937
PaeR7I CTCGAG 1 cut(s) 1609
PagI TCATGA 1 cut(s) 1027
PaqCI CACCTGC 1 cut(s) 2518
PciI ACATGT 1 cut(s) 887
PciSI GCTCTTC 1 cut(s) 1621
PctI GAATGC 1 cut(s) 1878
PdmI GAANNNNTTC 1 cut(s) 173
PflMI CCANNNNNTGG 1 cut(s) 344
PfoI TCCNGGA 1 cut(s) 814
PinAI ACCGGT 2 cut(s) 1645, 1920
PkrI GCNGC 6 cut(s) 46, 49, 621, 745, 1162, 1675
PmaCI CACGTG 1 cut(s) 2363
PmlI CACGTG 1 cut(s) 2363
Ppu21I YACGTR 1 cut(s) 2363
PpuMI RGGWCCY 1 cut(s) 809
PscI ACATGT 1 cut(s) 887
PshBI ATTAAT 1 cut(s) 2007
Psp124BI GAGCTC 1 cut(s) 1616
Psp5II RGGWCCY 1 cut(s) 809
Psp6I CCWGG 2 cut(s) 814, 1717
PspCI CACGTG 1 cut(s) 2363
PspGI CCWGG 2 cut(s) 814, 1717
PspN4I GGNNCC 5 cut(s) 243, 403, 810, 1143, 2508
PspOMI GGGCCC 1 cut(s) 401
PspPI GGNCC 5 cut(s) 241, 287, 401, 402, 809
PspPPI RGGWCCY 1 cut(s) 809
PstI CTGCAG 1 cut(s) 748
PsuI RGATCY 4 cut(s) 349, 1141, 1782, 2312
RsaI GTAC 7 cut(s) 971, 1063, 1157, 1373, 1628, 1830, 1988
RsaNI GTAC 7 cut(s) 970, 1062, 1156, 1372, 1627, 1829, 1987
RseI CAYNNNNRTG 1 cut(s) 886
SacI GAGCTC 1 cut(s) 1616
SacII CCGCGG 1 cut(s) 2553
SapI GCTCTTC 1 cut(s) 1621
SatI GCNGC 6 cut(s) 45, 48, 620, 744, 1161, 1674
Sau96I GGNCC 5 cut(s) 241, 287, 401, 402, 809
ScrFI CCNGG 4 cut(s) 816, 1719, 2536, 2537
SduI GDGCHC 4 cut(s) 405, 719, 895, 1616
SfaNI GCATC 8 cut(s) 1127, 1218, 1482, 1885, 1982, 2161, 2343, 2434
SfcI CTRYAG 1 cut(s) 744
Sfr274I CTCGAG 1 cut(s) 1609
Sfr303I CCGCGG 1 cut(s) 2553
SfuI TTCGAA 1 cut(s) 1937
SgrBI CCGCGG 1 cut(s) 2553
SinI GGWCC 2 cut(s) 287, 809
SlaI CTCGAG 1 cut(s) 1609
SmaI CCCGGG 1 cut(s) 2537
SmiMI CAYNNNNRTG 1 cut(s) 886
SmlI CTYRAG 5 cut(s) 86, 217, 1136, 1349, 1609
SmoI CTYRAG 5 cut(s) 86, 217, 1136, 1349, 1609
SpeI ACTAGT 1 cut(s) 2216
SsiI CCGC 5 cut(s) 626, 1764, 2040, 2550, 2552
SspI AATATT 3 cut(s) 904, 1457, 1656
SspMI CTAG 2 cut(s) 1928, 2217
SstI GAGCTC 1 cut(s) 1616
StyD4I CCNGG 4 cut(s) 814, 1717, 2534, 2535
StyI CCWWGG 3 cut(s) 858, 1880, 2257
TaiI ACGT 1 cut(s) 2365
TaqI TCGA 7 cut(s) 52, 252, 480, 1610, 1937, 2330, 2525
TatI WGTACW 3 cut(s) 1371, 1626, 1828
TscAI CASTG 2 cut(s) 1609, 2206
TseFI GTSAC 7 cut(s) 81, 124, 154, 846, 951, 1277, 2219
TseI GCWGC 6 cut(s) 44, 47, 619, 743, 1160, 1673
Tsp45I GTSAC 7 cut(s) 81, 124, 154, 846, 951, 1277, 2219
TspGWI ACGGA 2 cut(s) 2004, 2265
TspMI CCCGGG 1 cut(s) 2535
TspRI CASTG 2 cut(s) 1609, 2206
Van91I CCANNNNNTGG 1 cut(s) 344
VpaK11BI GGWCC 2 cut(s) 287, 809
VspI ATTAAT 1 cut(s) 2007
XapI RAATTY 7 cut(s) 255, 408, 652, 1068, 1325, 1366, 2030
XceI RCATGY 3 cut(s) 206, 891, 1399
XhoI CTCGAG 1 cut(s) 1609
XmaI CCCGGG 1 cut(s) 2535
XmiI GTMKAC 1 cut(s) 1507
XmnI GAANNNNTTC 1 cut(s) 173
XspI CTAG 2 cut(s) 1928, 2217
Zsp2I ATGCAT 1 cut(s) 1878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.