FvH4_1g27541

AAA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000279
Physical Location & Seq
Reverse (-)
19190254 .. 19193332
3079 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g27541.t1

Sequence Viewer

Length: 1500 bp
ATGTACCGATCTTATTCCTTCATAAATGTTGCCAATGGAAAAGATGAATTTGATCACAGATGTAGTCGGAAAAATATGGTGGAGGTTGCTGTAGTCTCTGAGATAGTAGCAAGCCTTTATAAAGAATTTAGAAGAACAAAGAAGAAAGTTAGTATTGGTGTCATATCACCATACAAGGCTCAAGTTTATGCAATTCAAAAGATACTCACAAAATCTACTGGAACTTCTGATACTGGCTTCTCTGTAAGTGTGCGATCTGTTGATGGGTTCCAAGGTGGTGAAGAAGATGTGATAATTATATCTACTGTGCGATGTAAATGGGAACGGGTCAGTTGGTTTTTTGTCAAACAGGCAAAGAGCAAATGTTGCACTTACACGTGCAAGTGGGTAATGCATCAACTTTGGTTACAAGTGACTCTGTTTGGAAGAAGCTTGTCCTTGATGCCAAAGAAACGGAACTGTTTTCATAATGCTGATGAAGACAGCAACTTGGCTCAGGCTATTACAACTGCCCTGCTAGAGCTTGACCAACTTCATTCTCTGCTTAATATCGGCTCTATGCTGTTCAAAAACGCTATATGGAAGGTTTGCTTCACTGGTGACTTTTTGAACTCCATAACAAAAATTAAAGATACTGCGATTCTAGGGGAAGTGCTTGCGTTATTAACCAGCCTTTCGCGTGGATGGCGCTCTCCTCACAAGGATAAAGGAAATGTAGTGTATGATGGAACTTCTGCTCAGCTGTTAGAGAAGTATAAAATCAAAGGGCACATGAATCTCATTTGGACTGTAGATATTATCCAGGAGAATGCACATTATGTCCAAGTTATAAAGTTTTGGGATATTTTGCCATTTTCTCATTTACCAGAACTAGCAAAGCGTCTTGACATTGTTTTTGGAAAATTTACAGTGGATAAGATGAACCGCTGCAAGCACAAATGCATTGACAGGGATACTGTTGTTCCAATGAGATGGCCGGTGGTTTTCACCAACTTCCCTATAGATGATCATGAGGAGTTCCTTTCAAAGCCATTGTCTTCTCTCATTATTACAGCTAATCCAAAAACGTCGACAACAACATATGGGGAGACAGCAGCAAAAGCAGTAAAATCGATTATCCATTCTAAGAACAATGTTATTAAAAAAGAAAGATTGTTATGGAAAATAAAGGCTGAAGACGGAGTGAAGGATTTCAGCACTTGCCTTGAAGCTGATCCTGTGGAGTTCTTATCAAAACCATTATCATCACTAAGTCTAGCAGATAAGCCAGAAGCATCTACTTCCACTGACATGGAGACAGCAAAAGCAGTAAAATCGATTATCCGTTCTAAGAACAACGTTAATAAAAAAGAAAGATTGTTATGGAAAATAAAGTCCGAAGAAGGAGTGAAGGATTTCAGCACTTGCCTTGAAGCTGATCCTGTGGAATTCTTGTCAAAACCATTATCATCACCAAGTCTAGCAGATAAGCCAGAAGCATCTACTTCCACTGACATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12

Protein Analysis

500

Amino Acids

56.42

Weight (kDa)

9.14

Isoelectric Point (pI)

32.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_12 PF13087 4 - 108 5.3e-22 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000279)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65810 AT1G65810 AT1G65810 AT4G05540 AT5G37140 AT5G37150 AT5G37160 AT5G37165 AT5G52090
fragaria_vesca FvH4_1g27541 FvH4_1g27542 FvH4_2g08960 FvH4_2g08960 FvH4_2g08960 FvH4_2g08960 FvH4_3g00170 FvH4_3g00170 FvH4_3g00170 FvH4_5g05930 FvH4_5g05930
malus_domestica MD08G1077700.v1.1 MD08G1077900.v1.1 MD08G1078000.v1.1 MD08G1078100.v1.1 MD08G1078300.v1.1 MD09G1057800.v1.1 MD09G1281400.v1.1 MD11G1222900.v1.1 MD14G1146400.v1.1
prunus_persica Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G202100_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.3G013100_v2.0.a1
pyrus_communis pycom09g18900 pycom09g18910 pycom13g26720 pycom13g26730 pycom13g26760 pycom13g26770
rosa_chinensis RchiOBHm_Chr3g0488001 RchiOBHm_Chr3g0488011 RchiOBHm_Chr3g0488031 RchiOBHm_Chr3g0488061 RchiOBHm_Chr3g0488111 RchiOBHm_Chr3g0488201 RchiOBHm_Chr3g0488211 RchiOBHm_Chr3g0488221 RchiOBHm_Chr3g0488321 RchiOBHm_Chr3g0488431 RchiOBHm_Chr6g0264601 RchiOBHm_Chr6g0264641
rosa_laevigata RLG00000014240 RLG00000014241 RLG00000022910 RLG00000022949 RLG00000030376
rosa_multiflora Rmu_co8075388.1_g000001 Rmu_co8424869.1_g000001 Rmu_co8427783.1_g000001 Rmu_sc0000546.1_g000002 Rmu_sc0000546.1_g000015 Rmu_sc0011052.1_g000014 Rmu_sc0017772.1_g000004
rosa_roxburghii Rroxscaffold_175G00431980 Rroxscaffold_175G00431990 Rroxscaffold_1G00034600 Rroxscaffold_6G00394230 Rroxscaffold_6G00394270 Rroxscaffold_6G00394280 Rroxscaffold_6G00394350 Rroxscaffold_7G00203240 Rroxscaffold_7G00203270 Rroxscaffold_7G00203280 Rroxscaffold_7G00203300 Rroxscaffold_7G00205740
rosa_rugosa Rorug03G0238000 Rorug03G0238100 Rorug03G0238100 Rorug03G0238500 Rorug05G0155400 Rorug05G0356000 Rorug05G0463000 Rorug06G0015200 Rorug06G0015300 Rorug06G0015400 Rorug06G0015500 Rorug06G0015500
rosa_samantha Rh1DG088100 Rh2AG244800 Rh2BG187500 Rh2CG247300 Rh3BG322700 Rh3BG322800 Rh3BG323100 Rh3BG323900 Rh3BG324000 Rh3BG324300 Rh3CG321300 Rh3CG321400 Rh3CG321500 Rh3DG319400 Rh3DG319900 Rh3DG320100 Rh3DG320200 Rh6AG133700 Rh6AG133800 Rh6AG133900 Rh6CG129300 Rh6CG129800 Rh6CG130200 Rh6DG116500 Rh6DG117200 Rh7DG378000
rosa_wichuraiana Rw2G014710 Rw3G025340 Rw3G025350 Rw3G025360 Rw5G020630 Rw6G011590 Rw6G011600 Rw7G041380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 120, 830
AccI GTMKAC 1 cut(s) 1070
AccII CGCG 1 cut(s) 679
AciI CCGC 1 cut(s) 925
AclI AACGTT 1 cut(s) 1338
AclWI GGATC 2 cut(s) 1208, 1412
AcoI YGGCCR 1 cut(s) 974
AcsI RAATTY 4 cut(s) 47, 125, 902, 1427
AcuI CTGAAG 1 cut(s) 1194
AcvI CACGTG 1 cut(s) 378
AfaI GTAC 1 cut(s) 5
AflIII ACRYGT 2 cut(s) 375, 1494
AgsI TTSAA 6 cut(s) 197, 568, 610, 1026, 1208, 1412
AjnI CCWGG 1 cut(s) 801
AluBI AGCT 6 cut(s) 432, 523, 742, 1055, 1211, 1415
AluI AGCT 6 cut(s) 432, 523, 742, 1055, 1211, 1415
Alw26I GTCTC 3 cut(s) 100, 1082, 1289
AlwI GGATC 2 cut(s) 1208, 1412
AoxI GGCC 1 cut(s) 974
ApeKI GCWGC 2 cut(s) 927, 1094
ApoI RAATTY 4 cut(s) 47, 125, 902, 1427
ArsI GACNNNNNNTTYG 4 cut(s) 878, 910, 1019, 1051
Asp700I GAANNNNTTC 2 cut(s) 1190, 1394
AspLEI GCGC 1 cut(s) 690
AsuHPI GGTGA 5 cut(s) 159, 290, 611, 979, 1443
BaeGI GKGCMC 1 cut(s) 771
BaeI ACNNNNGTAYC 2 cut(s) 945, 978
BbrPI CACGTG 1 cut(s) 378
BbsI GAAGAC 3 cut(s) 486, 1029, 1182
BbvI GCAGC 2 cut(s) 914, 1106
BccI CCATC 4 cut(s) 257, 678, 719, 966
BcgI CGANNNNNNTGC 4 cut(s) 1092, 1126, 1296, 1330
BciT130I CCWGG 1 cut(s) 803
BciVI GTATCC 1 cut(s) 946
BclI TGATCA 2 cut(s) 52, 1006
BcoDI GTCTC 3 cut(s) 100, 1082, 1289
BfaI CTAG 5 cut(s) 518, 644, 872, 1256, 1460
BfmI CTRYAG 3 cut(s) 90, 789, 999
BfoI RGCGCY 1 cut(s) 691
BfuI GTATCC 1 cut(s) 946
BisI GCNGC 2 cut(s) 928, 1095
BlpI GCTNAGC 1 cut(s) 738
BlsI GCNGC 2 cut(s) 929, 1096
Bme1390I CCNGG 1 cut(s) 803
BmiI GGNNCC 1 cut(s) 269
BmrFI CCNGG 1 cut(s) 803
BmsI GCATC 4 cut(s) 403, 432, 1283, 1487
BpiI GAAGAC 3 cut(s) 486, 1029, 1182
Bpu10I CCTNAGC 1 cut(s) 495
Bpu1102I GCTNAGC 1 cut(s) 738
BpuEI CTTGAG 1 cut(s) 165
Bsa29I ATCGAT 2 cut(s) 1112, 1316
BsaAI YACGTR 1 cut(s) 378
BsaJI CCNNGG 1 cut(s) 271
Bse118I RCCGGY 1 cut(s) 976
Bse1I ACTGG 3 cut(s) 223, 238, 601
BseBI CCWGG 1 cut(s) 803
BseCI ATCGAT 2 cut(s) 1112, 1316
BseDI CCNNGG 1 cut(s) 271
BseGI GGATG 1 cut(s) 689
BseMII CTCAG 3 cut(s) 90, 509, 752
BseNI ACTGG 3 cut(s) 223, 238, 601
BseRI GAGGAG 2 cut(s) 684, 1028
BseSI GKGCMC 1 cut(s) 771
BseXI GCAGC 2 cut(s) 914, 1106
Bsh1236I CGCG 1 cut(s) 679
BshFI GGCC 1 cut(s) 976
BshVI ATCGAT 2 cut(s) 1112, 1316
BsiSI CCGG 1 cut(s) 977
BsmAI GTCTC 3 cut(s) 100, 1082, 1289
BsmI GAATGC 1 cut(s) 814
BsnI GGCC 1 cut(s) 976
Bsp1286I GDGCHC 1 cut(s) 771
Bsp143I GATC 6 cut(s) 8, 52, 254, 1006, 1213, 1417
Bsp1720I GCTNAGC 1 cut(s) 738
BspACI CCGC 1 cut(s) 925
BspANI GGCC 1 cut(s) 976
BspCNI CTCAG 3 cut(s) 91, 508, 751
BspDI ATCGAT 2 cut(s) 1112, 1316
BspFNI CGCG 1 cut(s) 679
BspHI TCATGA 1 cut(s) 1009
BspLI GGNNCC 1 cut(s) 269
BspPI GGATC 2 cut(s) 1208, 1412
BsrFI RCCGGY 1 cut(s) 976
BsrI ACTGG 3 cut(s) 223, 238, 601
BssAI RCCGGY 1 cut(s) 976
BssECI CCNNGG 1 cut(s) 271
BssMI GATC 6 cut(s) 8, 52, 254, 1006, 1213, 1417
BssT1I CCWWGG 1 cut(s) 271
Bst2UI CCWGG 1 cut(s) 803
Bst4CI ACNGT 5 cut(s) 307, 461, 790, 910, 958
BstAPI GCANNNNNTGC 1 cut(s) 366
BstBAI YACGTR 1 cut(s) 378
BstC8I GCNNGC 3 cut(s) 112, 657, 932
BstDEI CTNAG 6 cut(s) 99, 495, 738, 1125, 1250, 1329
BstF5I GGATG 1 cut(s) 689
BstFNI CGCG 1 cut(s) 679
BstH2I RGCGCY 1 cut(s) 691
BstHHI GCGC 1 cut(s) 690
BstKTI GATC 6 cut(s) 11, 55, 257, 1009, 1216, 1420
BstMAI GTCTC 3 cut(s) 100, 1082, 1289
BstMBI GATC 6 cut(s) 8, 52, 254, 1006, 1213, 1417
BstMWI GCNNNNNNNGC 3 cut(s) 366, 685, 1100
BstNI CCWGG 1 cut(s) 803
BstNSI RCATGY 1 cut(s) 1498
BstSCI CCNGG 1 cut(s) 801
BstSFI CTRYAG 3 cut(s) 90, 789, 999
BstSLI GKGCMC 1 cut(s) 771
BstUI CGCG 1 cut(s) 679
BstV1I GCAGC 2 cut(s) 914, 1106
BstV2I GAAGAC 3 cut(s) 486, 1029, 1182
BstXI CCANNNNNNTGG 2 cut(s) 972, 1291
Bsu15I ATCGAT 2 cut(s) 1112, 1316
BsuI GTATCC 1 cut(s) 946
BsuRI GGCC 1 cut(s) 976
BsuTUI ATCGAT 2 cut(s) 1112, 1316
BtgZI GCGATG 1 cut(s) 325
BtsCI GGATG 1 cut(s) 689
BtsIMutI CAGTG 4 cut(s) 594, 915, 1284, 1488
Cac8I GCNNGC 3 cut(s) 112, 657, 932
CciI TCATGA 1 cut(s) 1009
CfoI GCGC 1 cut(s) 690
Cfr10I RCCGGY 1 cut(s) 976
ClaI ATCGAT 2 cut(s) 1112, 1316
CseI GACGC 1 cut(s) 869
Csp6I GTAC 1 cut(s) 4
CviAII CATG 4 cut(s) 772, 1010, 1291, 1495
CviQI GTAC 1 cut(s) 4
DdeI CTNAG 6 cut(s) 99, 495, 738, 1125, 1250, 1329
DpnI GATC 6 cut(s) 10, 54, 256, 1008, 1215, 1419
DpnII GATC 6 cut(s) 8, 52, 254, 1006, 1213, 1417
EaeI YGGCCR 1 cut(s) 974
Eco130I CCWWGG 1 cut(s) 271
Eco57I CTGAAG 1 cut(s) 1194
Eco72I CACGTG 1 cut(s) 378
EcoRI GAATTC 1 cut(s) 1427
EcoRII CCWGG 1 cut(s) 801
EcoT14I CCWWGG 1 cut(s) 271
EcoT22I ATGCAT 2 cut(s) 396, 944
ErhI CCWWGG 1 cut(s) 271
FaeI CATG 4 cut(s) 775, 1013, 1294, 1498
FalI AAGNNNNNCTT 2 cut(s) 575, 607
FatI CATG 4 cut(s) 771, 1009, 1290, 1494
FauNDI CATATG 1 cut(s) 1081
FbaI TGATCA 2 cut(s) 52, 1006
FblI GTMKAC 1 cut(s) 1070
Fnu4HI GCNGC 2 cut(s) 928, 1095
FokI GGATG 1 cut(s) 696
Fsp4HI GCNGC 2 cut(s) 928, 1095
FspBI CTAG 5 cut(s) 518, 644, 872, 1256, 1460
GlaI GCGC 1 cut(s) 689
GluI GCNGC 2 cut(s) 928, 1095
HaeII RGCGCY 1 cut(s) 691
HaeIII GGCC 1 cut(s) 976
HapII CCGG 1 cut(s) 977
HgaI GACGC 1 cut(s) 869
HhaI GCGC 1 cut(s) 690
Hin1II CATG 4 cut(s) 775, 1013, 1294, 1498
Hin6I GCGC 1 cut(s) 688
HinP1I GCGC 1 cut(s) 688
HincII GTYRAC 1 cut(s) 1071
HindII GTYRAC 1 cut(s) 1071
HindIII AAGCTT 1 cut(s) 430
HinfI GANTC 3 cut(s) 415, 640, 775
HpaII CCGG 1 cut(s) 977
HphI GGTGA 5 cut(s) 159, 290, 611, 979, 1443
Hpy166II GTNNAC 1 cut(s) 1071
Hpy188I TCNGA 4 cut(s) 69, 100, 229, 1378
Hpy188III TCNNGA 2 cut(s) 884, 1010
Hpy8I GTNNAC 1 cut(s) 1071
Hpy99I CGWCG 1 cut(s) 1072
HpyAV CCTTC 5 cut(s) 28, 577, 1180, 1376, 1384
HpyCH4III ACNGT 5 cut(s) 307, 461, 790, 910, 958
HpyCH4IV ACGT 3 cut(s) 377, 1067, 1338
HpyCH4V TGCA 7 cut(s) 191, 369, 381, 394, 812, 930, 942
HpyF10VI GCNNNNNNNGC 3 cut(s) 366, 685, 1100
HpyF3I CTNAG 6 cut(s) 99, 495, 738, 1125, 1250, 1329
HpySE526I ACGT 3 cut(s) 377, 1067, 1338
Hsp92II CATG 4 cut(s) 775, 1013, 1294, 1498
HspAI GCGC 1 cut(s) 688
Ksp22I TGATCA 2 cut(s) 52, 1006
Kzo9I GATC 6 cut(s) 8, 52, 254, 1006, 1213, 1417
Lsp1109I GCAGC 2 cut(s) 914, 1106
LweI GCATC 4 cut(s) 403, 432, 1283, 1487
MaeI CTAG 5 cut(s) 518, 644, 872, 1256, 1460
MaeII ACGT 3 cut(s) 377, 1067, 1338
MaeIII GTNAC 3 cut(s) 405, 412, 599
MalI GATC 6 cut(s) 10, 54, 256, 1008, 1215, 1419
MboI GATC 6 cut(s) 8, 52, 254, 1006, 1213, 1417
MboII GAAGA 9 cut(s) 144, 154, 293, 296, 438, 491, 1029, 1187, 1391
MhlI GDGCHC 1 cut(s) 771
MluCI AATT 7 cut(s) 47, 125, 192, 294, 624, 902, 1427
MlyI GAGTC 1 cut(s) 409
MmeI TCCRAC 1 cut(s) 47
MnlI CCTC 3 cut(s) 76, 705, 1006
Mph1103I ATGCAT 2 cut(s) 396, 944
MroXI GAANNNNTTC 2 cut(s) 1190, 1394
MseI TTAA 5 cut(s) 546, 627, 665, 1140, 1341
MslI CAYNNNNRTG 2 cut(s) 1289, 1493
MspA1I CMGCKG 2 cut(s) 742, 927
MspI CCGG 1 cut(s) 977
MspR9I CCNGG 1 cut(s) 803
Mva1269I GAATGC 1 cut(s) 814
MvaI CCWGG 1 cut(s) 803
MvnI CGCG 1 cut(s) 679
MwoI GCNNNNNNNGC 3 cut(s) 366, 685, 1100
NdeI CATATG 1 cut(s) 1081
NdeII GATC 6 cut(s) 8, 52, 254, 1006, 1213, 1417
NlaIII CATG 4 cut(s) 775, 1013, 1294, 1498
NlaIV GGNNCC 1 cut(s) 269
NmuCI GTSAC 2 cut(s) 412, 599
NsiI ATGCAT 2 cut(s) 396, 944
NspI RCATGY 1 cut(s) 1498
PagI TCATGA 1 cut(s) 1009
PciI ACATGT 1 cut(s) 1494
PctI GAATGC 1 cut(s) 814
PdmI GAANNNNTTC 2 cut(s) 1190, 1394
PfeI GAWTC 2 cut(s) 640, 775
PfoI TCCNGGA 1 cut(s) 801
PkrI GCNGC 2 cut(s) 929, 1096
PleI GAGTC 1 cut(s) 409
PmaCI CACGTG 1 cut(s) 378
PmlI CACGTG 1 cut(s) 378
PpsI GAGTC 1 cut(s) 409
Ppu21I YACGTR 1 cut(s) 378
PscI ACATGT 1 cut(s) 1494
PsiI TTATAA 2 cut(s) 120, 830
Psp1406I AACGTT 1 cut(s) 1338
Psp6I CCWGG 1 cut(s) 801
PspCI CACGTG 1 cut(s) 378
PspGI CCWGG 1 cut(s) 801
PspN4I GGNNCC 1 cut(s) 269
PsrI GAACNNNNNNTAC 2 cut(s) 214, 246
PvuII CAGCTG 1 cut(s) 742
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
RseI CAYNNNNRTG 2 cut(s) 1289, 1493
SalI GTCGAC 1 cut(s) 1069
SaqAI TTAA 5 cut(s) 546, 627, 665, 1140, 1341
SatI GCNGC 2 cut(s) 928, 1095
Sau3AI GATC 6 cut(s) 8, 52, 254, 1006, 1213, 1417
SchI GAGTC 1 cut(s) 409
ScrFI CCNGG 1 cut(s) 803
SduI GDGCHC 1 cut(s) 771
SfaNI GCATC 4 cut(s) 403, 432, 1283, 1487
SfcI CTRYAG 3 cut(s) 90, 789, 999
SmiMI CAYNNNNRTG 2 cut(s) 1289, 1493
SmlI CTYRAG 1 cut(s) 180
SmoI CTYRAG 1 cut(s) 180
Sse9I AATT 7 cut(s) 47, 125, 192, 294, 624, 902, 1427
SsiI CCGC 1 cut(s) 925
SspMI CTAG 5 cut(s) 518, 644, 872, 1256, 1460
StyD4I CCNGG 1 cut(s) 801
StyI CCWWGG 1 cut(s) 271
TaaI ACNGT 5 cut(s) 307, 461, 790, 910, 958
TaiI ACGT 3 cut(s) 380, 1070, 1341
TaqI TCGA 3 cut(s) 1070, 1112, 1316
TasI AATT 7 cut(s) 47, 125, 192, 294, 624, 902, 1427
TfiI GAWTC 2 cut(s) 640, 775
Tru1I TTAA 5 cut(s) 546, 627, 665, 1140, 1341
Tru9I TTAA 5 cut(s) 546, 627, 665, 1140, 1341
TscAI CASTG 4 cut(s) 601, 915, 1291, 1495
TseFI GTSAC 2 cut(s) 412, 599
TseI GCWGC 2 cut(s) 927, 1094
Tsp45I GTSAC 2 cut(s) 412, 599
TspDTI ATGAA 7 cut(s) 10, 60, 455, 492, 524, 788, 935
TspGWI ACGGA 3 cut(s) 469, 1194, 1313
TspRI CASTG 4 cut(s) 601, 915, 1291, 1495
XapI RAATTY 4 cut(s) 47, 125, 902, 1427
XceI RCATGY 1 cut(s) 1498
XmiI GTMKAC 1 cut(s) 1070
XmnI GAANNNNTTC 2 cut(s) 1190, 1394
XspI CTAG 5 cut(s) 518, 644, 872, 1256, 1460
Zsp2I ATGCAT 2 cut(s) 396, 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.