Rw3G025360

AAA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000279
Physical Location & Seq
Forward (+)
33762401 .. 33770994
8594 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G025360.1

Sequence Viewer

Length: 4350 bp
ATGGGGAAGAAGAGTAAGCTGAAACAGGAACTAGTTACAGATACTAGCAGCCTACTGGGTTTGGTATTCTCTTGGTCTATTTCGGATGTTCTCAATGAAAATCTTTACAGAAATCAGGTGCAGGGGATCCCCGACACATTTGTGACATCTACGAGTTACAAGAAATCATTCATTCCTTCACTTCTTGAGGAAACTCATGCTGATTTACTATCGAATATGAAGTCTCTGTCACATGCACCTACTTGTGAAATACTGTCAATCGAAGATTCAGCTAGACCTCCTGGTGACTTGTTTTACGATATAATGTATAATAGAGATACAGAAACTGATGAGAATCACAAAGGACCGATGTATGAGCCACAGGTTGGAGATATCATTGCCTTGACAAATGTTAGACCAAATTGCATTGATGATTTGAACAGGCCTCCAAGGTTCTATCTAATCGCTTATGTTCATAAAGCAAATGATATTGATGAATTTCCTGATGATCTCCAGTTCAAAATACTATCATCGAAGCCTATCAACTATGGAGAACCAGATATGCGTAAGAGCAATAGAGAAACACTTTTTGCTGTCTATCTTATGAACTTGATTACAAATCTCCGTGTATGGAAGGCTTTGAACTCAGAGGGAAATACAAATATCATTAACAAAGTTCTGCAACCCAAGTCAGATGATGGGGATTCTTGTTCAGTTTGCTTCCCCAGGGAAAAATGCTACACTGGCATTTCTGCTATATGGCCTACAATTTGCTCTCAAAATCTAAATGAATCCCAAGAAGCTGCGGTTTTGAACTGTATCAGTGTGGCTCAATGCCATCACCAGAATTCTGTAAAATTAATATGGGGTCCTCCTGGTACTGGCAAGACGAAGACAATGAGTCTGACACTCTTTGCTCTCTTTCATTTAAAGTGCAGAACTCTCACATGTGCTCCCACCAATATTGCGGTGCTAGAAGTAGCAGCAAGACTCCGGAGATTGGTTAACCAGTCACTCGAGTACGGCAGGTATGGACTAGGAGATATAGTTCTCTTTGGTAACAAGAAGCGAATGAAGATTGACAACAATGATGATCTTCATGACATATTTCTTGATCATCGTGTTAAAATCCTCATCAAGTGTTTGGTCCCTTTATCTGGGTGGAAACATTTGTTAGAATCAATGATACATTTACTTGATGATCCTGAGGAACAATATTCTTTGTATTTGAAAAAGAGAGTTGAAAAGCAGAAACAGAATGCACAAAAAAATGAGGAGGGTAATAAAAAACATAATGGAGATGAGGATTGTCCTTTGACATATGAGGAATTTGTAAACAATGAATTTGATTCAATCAGTCAGGAGCTGAAGATTTGTATGGTAGATTTGTACACCCACTTACCAACTTCTTGCATTTCACTTAAAGTGGTGAAGGGCATGGTCAGAGCTTTGGGTTTGCTCAAGTCTATTAAAACTTTATTGCATACTACTGGTGTTGCTAATGAAGGGTTGGAGTCAGTCTTCAAAGATTCCAAAGTTCCACGAAGCATTGTTGGTTCCCTTACGCAGTTGAGAACAAAGTGTACAAACACGCTAAAGTCCCTTCCAATGGAATTCTCTGTTCCTATTAATGAATATTCATTAAAGAACTTCTGCCTAGAAAATGCTTGCTTAATATTCTGTACTGCATCAACTTCTTCCAAATTGCATGATGTAACAGGAACAAGACCATTGGAATTGTTGATCATTGATGAAGCTGCTCAGCTTAAAGAATGTGAATCAGCAATTCCTTTACAACTATCTGGTCTCCGCCATGCTATCCTTGTAGGAGATGAGAGGCAACTCCCTGCCATGGTTAAAAGCGAGATTGCAGTAAGTGCTGATTTTGGAAGAAGTTTGTTTGGAAGGCTGGCAAAGTTGGGGCACAAGAAGCACCTACTCAATGTCCAGTACAGGATGCATCCATCCATCAGTTTATTTCCAAAAAGGGAGTTCTACGACAACCAGATAGTAGATGGTCCAAATGTCAAAGAAAGAAGCTATGGGAGGTGCTTCCTCAAGGGAAAAATGTACCAATCCTATTCCTTCATAAATGTAGCCGATGGAAAAGAAGAATTTGATCACAGATTTAGTCGGAAAAATATGGTGGAGGTTGCTGCAGTCTCTGGGATAGTAGCAAGCCTTTATAAAGAATTTATTGGAACAAAGAAGAAAGTTAGTATTGGGGTCATATCACCATACAAGGCTCAAGTTTATGCAATTCAAGAGATACTCAGAAAATATACTGAAACTTCTGATACTGGATTCTCCGTAAGTGTGAGATCTGTTGATGGGTTCCAAGGTGGTGAAGAAGATGTGATAATTATATCTACTGTCCGATGTAATGGGAACGGGTCAGTTGGTTTCTTGTCAAACCAGCAAAGAGCAAATGTTGCCTTAACACGTGCAAGGTATTGCCTTTGGATATTGGGGAATGCGTCAACTTTGATTGCTAGTGACTCTGTTTGGAAGAAGCTAGTCCTTGATGCCAAGAAACGGAACTGTTTTTATAATGCTGATGAGGACAGCAACTTGGCTCAGGCTATTACAGCTGCCCTGTTGGAGCTTGACCAACTTCATTCTCTGCTTAATGTCGACTCTCTGCTGTTCAAAAATGCTATATGGAAGGTTTACCTCACTGATGACTTTTTGAACTCCATAACAAAAATTAAAGACACTGTGATTCTTTGGGAAGTGCTTGCGTTATTAACCAAGCTTTCGAGTGGATGGCGCCGACCTCTTAAGGATAAAGGAACTATAGTGTATGATGGGACTTCTGCTCAACTGTTAGAGAAGTATAAAATCAAAGGGAACTTGAATCTCATTTGGACTGTCGATATTCTCCAGGAGAATGCACATTACATCCAAGTTATGAAGTTTTGGGATATTTTGCCATTTTCTCATATACCAGTACTAGCCAAGCGTCTTGACATTGTTTTTGGGAATTTTACAGTGAACAAGATGAACCGCTGCCTTCACAAATGCATTGACAGGGATACTGTTGTTCCAATGAGATGGCCGGTGGTTTTCAGCAATTACCCTATAGCTGATCATGAGGAGTTCCTTTCAAAACCATTGTCTTCTTTCAGTATAACAACTAATCGAGAAACAGCAACTTCAACATATGGGGAGACAGCAAAAGCGAAAAAAACAATTATCTCTTCAAAAAGCAATGTCAATGAAAAAGAAAGACGACTATGGAAAATAAAGGGTGAAGGAGTGGAGGATTTCAACACTTGCCTTGAAGCTAACCCTTCGGAGTTCTTGCCAAAACCATTATCATCACCAAGTCTAGCAGATAAGCCAGAAGCATCTACTTCCACTGACATGGAGACAGGGAAAGCAGTAAAACGATTTATCGCTTCGAAAACCAATGTCAATCGAAAAGAAAGATTGTTATGGAAAATAAAGGGTGAAGGAGTGAAGGATTTCAGCACTTGTGTTGAAGCTAACCCTGTGGAGTTCTTGTCAAAACCATTTTCATCTCCAAGTCTGTCAGATAAGCCAGAAGCATCTACTTCCACTGACATGGAGACAGCGAAAGCAGTAAAACCAATTATCGCTTCGAAAAGTAATGTCAATCAAAACGAAAAATTGTTATGGAAAATAAAGGGTGAAGAAGAAGTGAAGGATTTCAGCACTTGCGTTGAAGCTATCCCTGTGGAGTTCTTGTCAAAACCATTTTCATCACCAAGTCTATCAGATAAGCCAGAAGCATCTACTTCCACTGACATGGAGACGGCGAAAGCAGTAAAACCAATTATCCCTTCGAAAAGCAATGCCAATCAAAAAGAAAGATTGTTATGGAAAATAAAGGGTGAAGGAGTGAAGGATTTCAGCACTTGCCTTGAAGCTAACCCTGTGGAGTTCTTGTCAAAACCATTATCATCACTAAGTCTAGCAGATAAGCCAGAAGCATCTACTTCCACTGATAGGGAGACAGCGAAAGCAGTAAAACCAATTATCGCTTTGAAAAGCAATGTCAATCAAAAAGAAAAATTGTTATGGAAAATAAAGGGTGAAGAAGAAGTGAAGGATTTCAGCACTTGCGTTGAAGCTATCCCTGTGGAGTTCTTGCTAAAACCATTATCATCCCTAAGTCTAGCAGATAAGCCAGAAGCATCTACTTCCACTGACATGGAATCTGCGAATAGATCCGCTACCAAAAGCGTTTCAAAAAGCACTGCGAGTCGAAGAAGGAGATCATCATCAAAACTAAAAGTCGAAGACCATGTGTTCCTTAAGATTCGACCTTGTTGTGGAATTAAAAGGTATTCCCAAGGCGCAAAACTCACTCCACAGTTTACTTGTCCTTCTGAGGTTTCGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12

Protein Analysis

1449

Amino Acids

162.43

Weight (kDa)

8.64

Isoelectric Point (pI)

34.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 80 - 212 3.9e-14 Domain of unknown function (DUF6469)
AAA_11 PF13086 254 - 615 6.2e-33 AAA domain
AAA_12 PF13087 623 - 819 2.3e-56 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000279)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65810 AT1G65810 AT1G65810 AT4G05540 AT5G37140 AT5G37150 AT5G37160 AT5G37165 AT5G52090
fragaria_vesca FvH4_1g27541 FvH4_1g27542 FvH4_2g08960 FvH4_2g08960 FvH4_2g08960 FvH4_2g08960 FvH4_3g00170 FvH4_3g00170 FvH4_3g00170 FvH4_5g05930 FvH4_5g05930
malus_domestica MD08G1077700.v1.1 MD08G1077900.v1.1 MD08G1078000.v1.1 MD08G1078100.v1.1 MD08G1078300.v1.1 MD09G1057800.v1.1 MD09G1281400.v1.1 MD11G1222900.v1.1 MD14G1146400.v1.1
prunus_persica Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G202100_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.3G013100_v2.0.a1
pyrus_communis pycom09g18900 pycom09g18910 pycom13g26720 pycom13g26730 pycom13g26760 pycom13g26770
rosa_chinensis RchiOBHm_Chr3g0488001 RchiOBHm_Chr3g0488011 RchiOBHm_Chr3g0488031 RchiOBHm_Chr3g0488061 RchiOBHm_Chr3g0488111 RchiOBHm_Chr3g0488201 RchiOBHm_Chr3g0488211 RchiOBHm_Chr3g0488221 RchiOBHm_Chr3g0488321 RchiOBHm_Chr3g0488431 RchiOBHm_Chr6g0264601 RchiOBHm_Chr6g0264641
rosa_laevigata RLG00000014240 RLG00000014241 RLG00000022910 RLG00000022949 RLG00000030376
rosa_multiflora Rmu_co8075388.1_g000001 Rmu_co8424869.1_g000001 Rmu_co8427783.1_g000001 Rmu_sc0000546.1_g000002 Rmu_sc0000546.1_g000015 Rmu_sc0011052.1_g000014 Rmu_sc0017772.1_g000004
rosa_roxburghii Rroxscaffold_175G00431980 Rroxscaffold_175G00431990 Rroxscaffold_1G00034600 Rroxscaffold_6G00394230 Rroxscaffold_6G00394270 Rroxscaffold_6G00394280 Rroxscaffold_6G00394350 Rroxscaffold_7G00203240 Rroxscaffold_7G00203270 Rroxscaffold_7G00203280 Rroxscaffold_7G00203300 Rroxscaffold_7G00205740
rosa_rugosa Rorug03G0238000 Rorug03G0238100 Rorug03G0238100 Rorug03G0238500 Rorug05G0155400 Rorug05G0356000 Rorug05G0463000 Rorug06G0015200 Rorug06G0015300 Rorug06G0015400 Rorug06G0015500 Rorug06G0015500
rosa_samantha Rh1DG088100 Rh2AG244800 Rh2BG187500 Rh2CG247300 Rh3BG322700 Rh3BG322800 Rh3BG323100 Rh3BG323900 Rh3BG324000 Rh3BG324300 Rh3CG321300 Rh3CG321400 Rh3CG321500 Rh3DG319400 Rh3DG319900 Rh3DG320100 Rh3DG320200 Rh6AG133700 Rh6AG133800 Rh6AG133900 Rh6CG129300 Rh6CG129800 Rh6CG130200 Rh6DG116500 Rh6DG117200 Rh7DG378000
rosa_wichuraiana Rw2G014710 Rw3G025340 Rw3G025350 Rw3G025360 Rw5G020630 Rw6G011590 Rw6G011600 Rw7G041380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2166, 2529
Acc36I ACCTGC 1 cut(s) 996
AccB1I GGYRCC 1 cut(s) 2748
AccB7I CCANNNNNTGG 1 cut(s) 365
AccI GTMKAC 1 cut(s) 2613
AccIII TCCGGA 1 cut(s) 972
AciI CCGC 5 cut(s) 785, 947, 1789, 2986, 4176
AclWI GGATC 4 cut(s) 121, 134, 1175, 4167
AcoI YGGCCR 1 cut(s) 3035
AcsI RAATTY 8 cut(s) 476, 826, 1307, 1322, 1592, 2093, 2171, 2962
AcuI CTGAAG 1 cut(s) 1367
AcvI CACGTG 1 cut(s) 2423
AcyI GRCGYC 1 cut(s) 2749
AfaI GTAC 8 cut(s) 859, 1001, 1370, 1564, 1663, 1931, 2051, 2931
AfiI CCNNNNNNNGG 9 cut(s) 365, 860, 979, 1136, 1588, 1831, 2514, 3951, 4277
AflII CTTAAG 2 cut(s) 2759, 4259
AflIII ACRYGT 2 cut(s) 926, 2420
AhdI GACNNNNNGTC 1 cut(s) 879
AhlI ACTAGT 1 cut(s) 31
AjnI CCWGG 4 cut(s) 280, 704, 853, 2862
AjuI GAANNNNNNNTTGG 4 cut(s) 3565, 3597, 3769, 3801
AleI CACNNNNGTG 1 cut(s) 140
AloI GAACNNNNNNTCC 2 cut(s) 1011, 1043
Alw21I GWGCWC 1 cut(s) 934
Alw26I GTCTC 8 cut(s) 228, 1790, 2146, 3143, 3344, 3545, 3749, 3950
AlwI GGATC 4 cut(s) 121, 134, 1175, 4167
AlwNI CAGNNNCTG 3 cut(s) 326, 1345, 2144
Ama87I CYCGRG 1 cut(s) 995
Aor13HI TCCGGA 1 cut(s) 972
AoxI GGCC 3 cut(s) 422, 740, 3035
ApeKI GCWGC 7 cut(s) 48, 782, 962, 1736, 2135, 2570, 2988
ApoI RAATTY 8 cut(s) 476, 826, 1307, 1322, 1592, 2093, 2171, 2962
ArsI GACNNNNNNTTYG 6 cut(s) 206, 238, 2939, 2971, 3080, 3112
AseI ATTAAT 2 cut(s) 839, 1608
Asp700I GAANNNNTTC 5 cut(s) 167, 3446, 3650, 3851, 4055
AspLEI GCGC 2 cut(s) 2751, 4304
AspS9I GGNCC 4 cut(s) 344, 848, 1126, 1997
AsuII TTCGAA 3 cut(s) 3383, 3584, 3788
AvaI CYCGRG 1 cut(s) 995
AvaII GGWCC 4 cut(s) 344, 848, 1126, 1997
AxyI CCTNAGG 1 cut(s) 1185
BaeGI GKGCMC 1 cut(s) 1905
BaeI ACNNNNGTAYC 2 cut(s) 3006, 3039
BamHI GGATCC 1 cut(s) 126
BanI GGYRCC 1 cut(s) 2748
BbrPI CACGTG 1 cut(s) 2423
BbsI GAAGAC 4 cut(s) 878, 1492, 3090, 4251
Bbv12I GWGCWC 1 cut(s) 934
BbvI GCAGC 7 cut(s) 60, 769, 974, 1723, 2122, 2557, 2975
BceAI ACGGC 2 cut(s) 1018, 3774
BciT130I CCWGG 4 cut(s) 282, 706, 855, 2864
BciVI GTATCC 1 cut(s) 3007
BclI TGATCA 4 cut(s) 1093, 1722, 2098, 3067
BcoDI GTCTC 8 cut(s) 228, 1790, 2146, 3143, 3344, 3545, 3749, 3950
BcuI ACTAGT 1 cut(s) 31
BfmI CTRYAG 3 cut(s) 2136, 2775, 3060
BfoI RGCGCY 1 cut(s) 2752
BfrI CTTAAG 2 cut(s) 2759, 4259
BfuAI ACCTGC 1 cut(s) 996
BfuI GTATCC 1 cut(s) 3007
BglII AGATCT 1 cut(s) 2300
BisI GCNGC 7 cut(s) 49, 783, 963, 1737, 2136, 2571, 2989
BlpI GCTNAGC 1 cut(s) 1740
BlsI GCNGC 7 cut(s) 50, 784, 964, 1738, 2137, 2572, 2990
BmcAI AGTACT 1 cut(s) 2931
Bme1390I CCNGG 4 cut(s) 282, 706, 855, 2864
Bme18I GGWCC 4 cut(s) 344, 848, 1126, 1997
BmeRI GACNNNNNGTC 1 cut(s) 879
BmeT110I CYCGRG 1 cut(s) 995
BmgT120I GGNCC 4 cut(s) 344, 848, 1126, 1997
BmiI GGNNCC 6 cut(s) 128, 849, 1128, 1537, 2315, 2750
BmrFI CCNGG 4 cut(s) 282, 706, 855, 2864
BmrI ACTGGG 1 cut(s) 65
BmsI GCATC 9 cut(s) 1676, 1926, 1948, 2494, 3338, 3539, 3743, 3944, 4148
BmuI ACTGGG 1 cut(s) 65
BpiI GAAGAC 4 cut(s) 878, 1492, 3090, 4251
BpmI CTGGAG 2 cut(s) 476, 2846
Bpu10I CCTNAGC 1 cut(s) 2556
Bpu1102I GCTNAGC 1 cut(s) 1740
Bpu14I TTCGAA 3 cut(s) 3383, 3584, 3788
BpuEI CTTGAG 4 cut(s) 206, 1424, 2021, 2211
BsaAI YACGTR 1 cut(s) 2423
BsaBI GATNNNNATC 2 cut(s) 333, 4225
BsaHI GRCGYC 1 cut(s) 2749
BsaI GGTCTC 1 cut(s) 1790
BsaJI CCNNGG 6 cut(s) 428, 704, 705, 1830, 2317, 4297
BsaWI WCCGGW 1 cut(s) 972
Bsc4I CCNNNNNNNGG 9 cut(s) 365, 860, 979, 1136, 1588, 1831, 2514, 3951, 4277
Bse118I RCCGGY 1 cut(s) 3037
Bse1I ACTGG 9 cut(s) 60, 493, 727, 865, 988, 1474, 1927, 2284, 2927
Bse21I CCTNAGG 1 cut(s) 1185
Bse3DI GCAATG 4 cut(s) 375, 3196, 3802, 4003
Bse8I GATNNNNATC 2 cut(s) 333, 4225
BseAI TCCGGA 1 cut(s) 972
BseBI CCWGG 4 cut(s) 282, 706, 855, 2864
BseDI CCNNGG 6 cut(s) 428, 704, 705, 1830, 2317, 4297
BseGI GGATG 7 cut(s) 91, 1939, 1941, 1943, 2750, 2880, 4109
BseJI GATNNNNATC 2 cut(s) 333, 4225
BseLI CCNNNNNNNGG 9 cut(s) 365, 860, 979, 1136, 1588, 1831, 2514, 3951, 4277
BseMI GCAATG 4 cut(s) 375, 3196, 3802, 4003
BseMII CTCAG 6 cut(s) 639, 1176, 1754, 2266, 2570, 4326
BseNI ACTGG 9 cut(s) 60, 493, 727, 865, 988, 1474, 1927, 2284, 2927
BseRI GAGGAG 2 cut(s) 1268, 3089
BseSI GKGCMC 1 cut(s) 1905
BseXI GCAGC 7 cut(s) 60, 769, 974, 1723, 2122, 2557, 2975
BsgI GTGCAG 2 cut(s) 140, 934
BshFI GGCC 3 cut(s) 424, 742, 3037
BshNI GGYRCC 1 cut(s) 2748
BsiHKAI GWGCWC 1 cut(s) 934
BsiHKCI CYCGRG 1 cut(s) 995
BsiSI CCGG 2 cut(s) 973, 3038
BslFI GGGAC 3 cut(s) 1112, 1564, 2803
BslI CCNNNNNNNGG 9 cut(s) 365, 860, 979, 1136, 1588, 1831, 2514, 3951, 4277
BsmAI GTCTC 8 cut(s) 228, 1790, 2146, 3143, 3344, 3545, 3749, 3950
BsmBI CGTCTC 1 cut(s) 3749
BsmFI GGGAC 3 cut(s) 1112, 1564, 2803
BsmI GAATGC 3 cut(s) 1243, 2458, 2875
BsnI GGCC 3 cut(s) 424, 742, 3037
Bso31I GGTCTC 1 cut(s) 1790
BsoBI CYCGRG 1 cut(s) 995
Bsp119I TTCGAA 3 cut(s) 3383, 3584, 3788
Bsp1286I GDGCHC 2 cut(s) 934, 1905
Bsp13I TCCGGA 1 cut(s) 972
Bsp1407I TGTACA 2 cut(s) 1368, 1562
Bsp1720I GCTNAGC 1 cut(s) 1740
Bsp19I CCATGG 1 cut(s) 1830
BspACI CCGC 5 cut(s) 785, 947, 1789, 2986, 4176
BspANI GGCC 3 cut(s) 424, 742, 3037
BspCNI CTCAG 6 cut(s) 638, 1177, 1753, 2265, 2569, 4327
BspEI TCCGGA 1 cut(s) 972
BspHI TCATGA 2 cut(s) 1078, 3070
BspLI GGNNCC 6 cut(s) 128, 849, 1128, 1537, 2315, 2750
BspMAI CTGCAG 1 cut(s) 2140
BspMI ACCTGC 1 cut(s) 996
BspPI GGATC 4 cut(s) 121, 134, 1175, 4167
BspT104I TTCGAA 3 cut(s) 3383, 3584, 3788
BspT107I GGYRCC 1 cut(s) 2748
BspTI CTTAAG 2 cut(s) 2759, 4259
BspTNI GGTCTC 1 cut(s) 1790
BsrDI GCAATG 4 cut(s) 375, 3196, 3802, 4003
BsrFI RCCGGY 1 cut(s) 3037
BsrGI TGTACA 2 cut(s) 1368, 1562
BsrI ACTGG 9 cut(s) 60, 493, 727, 865, 988, 1474, 1927, 2284, 2927
BssAI RCCGGY 1 cut(s) 3037
BssECI CCNNGG 6 cut(s) 428, 704, 705, 1830, 2317, 4297
BssNI GRCGYC 1 cut(s) 2749
BssT1I CCWWGG 4 cut(s) 428, 1830, 2317, 4297
Bst2UI CCWGG 4 cut(s) 282, 706, 855, 2864
Bst6I CTCTTC 2 cut(s) 5, 3184
BstACI GRCGYC 1 cut(s) 2749
BstAFI CTTAAG 2 cut(s) 2759, 4259
BstAPI GCANNNNNTGC 2 cut(s) 1856, 2411
BstAUI TGTACA 2 cut(s) 1368, 1562
BstBAI YACGTR 1 cut(s) 2423
BstBI TTCGAA 3 cut(s) 3383, 3584, 3788
BstC8I GCNNGC 4 cut(s) 1648, 1890, 2158, 2718
BstDEI CTNAG 8 cut(s) 625, 1185, 1740, 2252, 2556, 3911, 4115, 4335
BstDSI CCRYGG 1 cut(s) 1830
BstF5I GGATG 7 cut(s) 91, 1939, 1941, 1943, 2750, 2880, 4109
BstH2I RGCGCY 1 cut(s) 2752
BstHHI GCGC 2 cut(s) 2751, 4304
BstMAI GTCTC 8 cut(s) 228, 1790, 2146, 3143, 3344, 3545, 3749, 3950
BstMWI GCNNNNNNNGC 5 cut(s) 723, 1856, 1909, 2411, 2567
BstNI CCWGG 4 cut(s) 282, 706, 855, 2864
BstNSI RCATGY 2 cut(s) 236, 930
BstSCI CCNGG 4 cut(s) 280, 704, 853, 2862
BstSFI CTRYAG 3 cut(s) 2136, 2775, 3060
BstSLI GKGCMC 1 cut(s) 1905
BstV1I GCAGC 7 cut(s) 60, 769, 974, 1723, 2122, 2557, 2975
BstV2I GAAGAC 4 cut(s) 878, 1492, 3090, 4251
BstX2I RGATCY 3 cut(s) 126, 2300, 4172
BstXI CCANNNNNNTGG 5 cut(s) 3033, 3346, 3547, 3751, 4156
BstYI RGATCY 3 cut(s) 126, 2300, 4172
Bsu36I CCTNAGG 1 cut(s) 1185
BsuI GTATCC 1 cut(s) 3007
BsuRI GGCC 3 cut(s) 424, 742, 3037
BtgI CCRYGG 1 cut(s) 1830
BtsCI GGATG 7 cut(s) 91, 1939, 1941, 1943, 2750, 2880, 4109
BtsI GCAGTG 1 cut(s) 4200
BveI ACCTGC 1 cut(s) 996
Cac8I GCNNGC 4 cut(s) 1648, 1890, 2158, 2718
CaiI CAGNNNCTG 3 cut(s) 326, 1345, 2144
CciI TCATGA 2 cut(s) 1078, 3070
CfoI GCGC 2 cut(s) 2751, 4304
Cfr10I RCCGGY 1 cut(s) 3037
Cfr13I GGNCC 4 cut(s) 344, 848, 1126, 1997
CseI GACGC 2 cut(s) 2445, 2930
Csp6I GTAC 8 cut(s) 858, 1000, 1369, 1563, 1662, 1930, 2050, 2930
CspCI CAANNNNNGTGG 2 cut(s) 925, 960
CviQI GTAC 8 cut(s) 858, 1000, 1369, 1563, 1662, 1930, 2050, 2930
DdeI CTNAG 8 cut(s) 625, 1185, 1740, 2252, 2556, 3911, 4115, 4335
DinI GGCGCC 1 cut(s) 2750
DraI TTTAAA 1 cut(s) 909
DriI GACNNNNNGTC 1 cut(s) 879
EaeI YGGCCR 1 cut(s) 3035
Eam1104I CTCTTC 2 cut(s) 5, 3184
Eam1105I GACNNNNNGTC 1 cut(s) 879
EarI CTCTTC 2 cut(s) 5, 3184
EciI GGCGGA 1 cut(s) 1778
Eco130I CCWWGG 4 cut(s) 428, 1830, 2317, 4297
Eco147I AGGCCT 1 cut(s) 424
Eco31I GGTCTC 1 cut(s) 1790
Eco32I GATATC 1 cut(s) 373
Eco47I GGWCC 4 cut(s) 344, 848, 1126, 1997
Eco57I CTGAAG 1 cut(s) 1367
Eco72I CACGTG 1 cut(s) 2423
Eco81I CCTNAGG 1 cut(s) 1185
Eco88I CYCGRG 1 cut(s) 995
EcoO109I RGGNCCY 1 cut(s) 848
EcoRI GAATTC 2 cut(s) 826, 1592
EcoRII CCWGG 4 cut(s) 280, 704, 853, 2862
EcoRV GATATC 1 cut(s) 373
EcoT14I CCWWGG 4 cut(s) 428, 1830, 2317, 4297
EcoT22I ATGCAT 2 cut(s) 1941, 3005
EgeI GGCGCC 1 cut(s) 2750
EheI GGCGCC 1 cut(s) 2750
ErhI CCWWGG 4 cut(s) 428, 1830, 2317, 4297
Esp3I CGTCTC 1 cut(s) 3749
FaqI GGGAC 3 cut(s) 1112, 1564, 2803
FauNDI CATATG 2 cut(s) 1300, 3142
FbaI TGATCA 4 cut(s) 1093, 1722, 2098, 3067
FblI GTMKAC 1 cut(s) 2613
Fnu4HI GCNGC 7 cut(s) 49, 783, 963, 1737, 2136, 2571, 2989
FokI GGATG 7 cut(s) 98, 1926, 1930, 1948, 2757, 2867, 4096
Fsp4HI GCNGC 7 cut(s) 49, 783, 963, 1737, 2136, 2571, 2989
GlaI GCGC 2 cut(s) 2750, 4303
GluI GCNGC 7 cut(s) 49, 783, 963, 1737, 2136, 2571, 2989
GsuI CTGGAG 2 cut(s) 476, 2846
HaeII RGCGCY 1 cut(s) 2752
HaeIII GGCC 3 cut(s) 424, 742, 3037
HapII CCGG 2 cut(s) 973, 3038
HgaI GACGC 2 cut(s) 2445, 2930
HhaI GCGC 2 cut(s) 2751, 4304
Hin1I GRCGYC 1 cut(s) 2749
Hin6I GCGC 2 cut(s) 2749, 4302
HinP1I GCGC 2 cut(s) 2749, 4302
HincII GTYRAC 3 cut(s) 985, 2460, 2614
HindII GTYRAC 3 cut(s) 985, 2460, 2614
HindIII AAGCTT 1 cut(s) 2732
HpaI GTTAAC 1 cut(s) 985
HpaII CCGG 2 cut(s) 973, 3038
Hpy166II GTNNAC 9 cut(s) 985, 1315, 1371, 1563, 2460, 2614, 2650, 2974, 4323
Hpy8I GTNNAC 9 cut(s) 985, 1315, 1371, 1563, 2460, 2614, 2650, 2974, 4323
HpyCH4IV ACGT 1 cut(s) 2422
HpyF10VI GCNNNNNNNGC 5 cut(s) 723, 1856, 1909, 2411, 2567
HpyF3I CTNAG 8 cut(s) 625, 1185, 1740, 2252, 2556, 3911, 4115, 4335
HpySE526I ACGT 1 cut(s) 2422
Hsp92I GRCGYC 1 cut(s) 2749
HspAI GCGC 2 cut(s) 2749, 4302
KasI GGCGCC 1 cut(s) 2748
Kpn2I TCCGGA 1 cut(s) 972
Ksp22I TGATCA 4 cut(s) 1093, 1722, 2098, 3067
KspAI GTTAAC 1 cut(s) 985
LmnI GCTCC 3 cut(s) 937, 1342, 2581
Lsp1109I GCAGC 7 cut(s) 60, 769, 974, 1723, 2122, 2557, 2975
LweI GCATC 9 cut(s) 1676, 1926, 1948, 2494, 3338, 3539, 3743, 3944, 4148
MaeII ACGT 1 cut(s) 2422
MaeIII GTNAC 9 cut(s) 34, 142, 155, 228, 284, 990, 1037, 1693, 2474
MflI RGATCY 3 cut(s) 126, 2300, 4172
MhlI GDGCHC 2 cut(s) 934, 1905
Mly113I GGCGCC 1 cut(s) 2749
MlyI GAGTC 6 cut(s) 889, 963, 1502, 2471, 2609, 4216
MmeI TCCRAC 4 cut(s) 346, 1470, 2093, 2559
Mph1103I ATGCAT 2 cut(s) 1941, 3005
MroI TCCGGA 1 cut(s) 972
MroXI GAANNNNTTC 5 cut(s) 167, 3446, 3650, 3851, 4055
MslI CAYNNNNRTG 5 cut(s) 140, 3344, 3545, 3749, 4154
MspA1I CMGCKG 2 cut(s) 2570, 2988
MspCI CTTAAG 2 cut(s) 2759, 4259
MspI CCGG 2 cut(s) 973, 3038
MspR9I CCNGG 4 cut(s) 282, 706, 855, 2864
Mva1269I GAATGC 3 cut(s) 1243, 2458, 2875
MvaI CCWGG 4 cut(s) 282, 706, 855, 2864
MwoI GCNNNNNNNGC 5 cut(s) 723, 1856, 1909, 2411, 2567
NarI GGCGCC 1 cut(s) 2749
NcoI CCATGG 1 cut(s) 1830
NdeI CATATG 2 cut(s) 1300, 3142
NlaIV GGNNCC 6 cut(s) 128, 849, 1128, 1537, 2315, 2750
NmuCI GTSAC 5 cut(s) 142, 228, 284, 990, 2474
NsiI ATGCAT 2 cut(s) 1941, 3005
NspI RCATGY 2 cut(s) 236, 930
NspV TTCGAA 3 cut(s) 3383, 3584, 3788
OliI CACNNNNGTG 1 cut(s) 140
PaeR7I CTCGAG 1 cut(s) 995
PagI TCATGA 2 cut(s) 1078, 3070
PasI CCCWGGG 1 cut(s) 705
PceI AGGCCT 1 cut(s) 424
PciI ACATGT 1 cut(s) 926
PctI GAATGC 3 cut(s) 1243, 2458, 2875
PdmI GAANNNNTTC 5 cut(s) 167, 3446, 3650, 3851, 4055
PflMI CCANNNNNTGG 1 cut(s) 365
PfoI TCCNGGA 1 cut(s) 2862
PkrI GCNGC 7 cut(s) 50, 784, 964, 1738, 2137, 2572, 2990
PleI GAGTC 6 cut(s) 888, 963, 1501, 2471, 2609, 4215
PluTI GGCGCC 1 cut(s) 2752
PmaCI CACGTG 1 cut(s) 2423
PmlI CACGTG 1 cut(s) 2423
PpsI GAGTC 6 cut(s) 888, 963, 1501, 2471, 2609, 4215
Ppu21I YACGTR 1 cut(s) 2423
PpuMI RGGWCCY 1 cut(s) 848
PscI ACATGT 1 cut(s) 926
PshBI ATTAAT 2 cut(s) 839, 1608
PsiI TTATAA 2 cut(s) 2166, 2529
Psp5II RGGWCCY 1 cut(s) 848
Psp6I CCWGG 4 cut(s) 280, 704, 853, 2862
PspCI CACGTG 1 cut(s) 2423
PspGI CCWGG 4 cut(s) 280, 704, 853, 2862
PspN4I GGNNCC 6 cut(s) 128, 849, 1128, 1537, 2315, 2750
PspPI GGNCC 4 cut(s) 344, 848, 1126, 1997
PspPPI RGGWCCY 1 cut(s) 848
PspXI VCTCGAGB 1 cut(s) 995
PsrI GAACNNNNNNTAC 2 cut(s) 1546, 1578
PstI CTGCAG 1 cut(s) 2140
PstNI CAGNNNCTG 3 cut(s) 326, 1345, 2144
PsuI RGATCY 3 cut(s) 126, 2300, 4172
PvuII CAGCTG 1 cut(s) 2570
RsaI GTAC 8 cut(s) 859, 1001, 1370, 1564, 1663, 1931, 2051, 2931
RsaNI GTAC 8 cut(s) 858, 1000, 1369, 1563, 1662, 1930, 2050, 2930
RseI CAYNNNNRTG 5 cut(s) 140, 3344, 3545, 3749, 4154
SalI GTCGAC 1 cut(s) 2612
SatI GCNGC 7 cut(s) 49, 783, 963, 1737, 2136, 2571, 2989
Sau96I GGNCC 4 cut(s) 344, 848, 1126, 1997
ScaI AGTACT 1 cut(s) 2931
SchI GAGTC 6 cut(s) 889, 963, 1502, 2471, 2609, 4216
ScrFI CCNGG 4 cut(s) 282, 706, 855, 2864
SduI GDGCHC 2 cut(s) 934, 1905
SfaNI GCATC 9 cut(s) 1676, 1926, 1948, 2494, 3338, 3539, 3743, 3944, 4148
SfcI CTRYAG 3 cut(s) 2136, 2775, 3060
SfoI GGCGCC 1 cut(s) 2750
Sfr274I CTCGAG 1 cut(s) 995
SfuI TTCGAA 3 cut(s) 3383, 3584, 3788
SinI GGWCC 4 cut(s) 344, 848, 1126, 1997
SlaI CTCGAG 1 cut(s) 995
SmiMI CAYNNNNRTG 5 cut(s) 140, 3344, 3545, 3749, 4154
SmlI CTYRAG 7 cut(s) 185, 995, 1439, 2036, 2226, 2759, 4259
SmoI CTYRAG 7 cut(s) 185, 995, 1439, 2036, 2226, 2759, 4259
SpeI ACTAGT 1 cut(s) 31
SseBI AGGCCT 1 cut(s) 424
SsiI CCGC 5 cut(s) 785, 947, 1789, 2986, 4176
SspDI GGCGCC 1 cut(s) 2748
SspI AATATT 4 cut(s) 943, 1196, 1616, 1656
StuI AGGCCT 1 cut(s) 424
StyD4I CCNGG 4 cut(s) 280, 704, 853, 2862
StyI CCWWGG 4 cut(s) 428, 1830, 2317, 4297
TaiI ACGT 1 cut(s) 2425
TaqII GACCGA 1 cut(s) 361
TatI WGTACW 5 cut(s) 1368, 1562, 1661, 1929, 2929
TseFI GTSAC 5 cut(s) 142, 228, 284, 990, 2474
TseI GCWGC 7 cut(s) 48, 782, 962, 1736, 2135, 2570, 2988
Tsp45I GTSAC 5 cut(s) 142, 228, 284, 990, 2474
TspGWI ACGGA 3 cut(s) 593, 2278, 2530
Van91I CCANNNNNTGG 1 cut(s) 365
Vha464I CTTAAG 2 cut(s) 2759, 4259
VpaK11BI GGWCC 4 cut(s) 344, 848, 1126, 1997
VspI ATTAAT 2 cut(s) 839, 1608
XapI RAATTY 8 cut(s) 476, 826, 1307, 1322, 1592, 2093, 2171, 2962
XceI RCATGY 2 cut(s) 236, 930
XcmI CCANNNNNNNNNTGG 1 cut(s) 1991
XhoI CTCGAG 1 cut(s) 995
XmiI GTMKAC 1 cut(s) 2613
XmnI GAANNNNTTC 5 cut(s) 167, 3446, 3650, 3851, 4055
ZrmI AGTACT 1 cut(s) 2931
Zsp2I ATGCAT 2 cut(s) 1941, 3005
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.