RchiOBHm_Chr3g0488111

AAA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000279
Physical Location & Seq
Forward (+)
35669772 .. 35678636
8865 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45250

Sequence Viewer

Length: 3576 bp
ATGGGGAAGAAGAGTAAGCTGAAACAGGAACTAGTTACAGATACTAGCAGCCTAGTGGGTTTGGTATTCTCTTGGTCTATTTCGGATGTTCTCAATGAAAATCTTTACAGAAATCAGGTGCAGAGGATCCCCGACACATTTGTGACTTTGACGAGTTACAAGAAATCATTCATTCCTTCACTTGTTGAGGAAACTCATGCTGATTTACTATCGAATATGAAGACTCTGTCACATGCACCTACTTGTGAAATTCTGACAATTGAAGATTCAGCTGGACCTCCTGGTGACTTGTTTTACGATATAACGTATTATAGAGATACAGAAACTGATGAGAATCACAAAGGACCGATATATGAGCCACAGGTTGGAGATATCATTGCCTTGACAAATGTTAGACCAAAATGCATTGATGATTTGAACAGGCCTCCAAGGTTCTATCTAATTGCTTATGTTGATGAGGCAAAAGATGTTGATGAATTTCCTGATGATCTCCAGTTCAAAATACTATCATCGAAGCCTATCAACTATGGAGAACCAGACATGCATACGAGCAAGAGAGAAACACTTTTTGCTGTCTATCTTATGAACTTGACAACAAATCTCCGTGTATGGAAGGCTTTGAACTCAGAGGGAAATACAAATATCATTAACAAAGTTCTGCAACCCAAGTCAGATGATGGGGAATCTTGTTCAGTTTGCTTCTCCGGGGAATCTTGTTTAGTTTGCTTCTCCAAAGAAAAATGCAATGCTGGCATTTCTGCTATATGGCCTACAATTTGCTCTCAAAATCTAAATGAATCCCAAGAGGCTGCAGTTTTGAACTGTATCAGTTTGGCTCAATGTCATCACCAGAATTCTGTAAAATTAATATGGGGTCCTCCTGGTACTGGCAAGACGAAGACAATGAGTCTGACACTTTTTGCTCTCTTTCAGTTAAAGTGCAGAACTCTCACATGTGCTCCCACCAATATTGCAGTGCTAGAAGTAGCCACAAGACTCCGGAGATTGGTTAACCAGTCACTCGAGTATGGCAGGTATGGACTAGGAGATATAGTTCTCTTTGGTAACAAGAAGCGAATGAAGATTGACAACAATGATGATCTTCGTGACATATTTCTTGATCATCGTGTTAAAATCCTCATCAAGTGTTTGGTCCCTTTATCTGGGTGGAAACATTTGTTAGAATCAATGATACATTTACTTGTTGATCCTGAGGAACAATATTCTTTGTATTTGGAAAAGAAAGCTGAAAAGCAGAAACAGAATGCACAAAAAAATGAGGAGGATAATCGAAAACATAATGGAGATGAAGATTGTCCTTTGACATTTGTGGAGTTTGTGAAGAATGAATTTGATTCGATCAGTCAGGAGCTGAAGATTTGTATGGTAAATTTGTACACCCACTTACCAACTTCTTGCATTTCACTCAAAGTGGTGAAGGACATGGTCAGAGCTTCGGGTTTGCTCAAATCTATTGAATCTTCATTGCATTCTATTCGTGTTGCTAATGAAGGGTTTAAGTTACTCAAAGATTTCAAAGTTCCTAGAACTGTTGTTCGTCGCCTTATGCAGTTGAGAACAGAGTGTACAAACACACTAAAGTCGCTTCCAATGGAATTCTCTGTTCCTAGTATTAACAAATATGCATTAAAGAACTTCTGCCTAGAAAATGCTTGCTTAATATTCTGTACTGCATCAACTTCTTCCAAATTGCATGTTGTAGCAGGAACAAGACCATTGGAGTTGTTGGTCATTGATGAAGCTGCTCAGCTTAAAGAATGTGAATCAGCAATTCCTTTACAACTATCTGGTCTCCGCCATGCTATCCTTGTAGGAGATGAGAGGCAACTCCCTGCAATGGTCAAAAGCGAGATTGCAGCAAGTGCTGATTTTGGAAGAAGTTTGTTTGGAAGACTGGCAAAGTTGGGATACAAGAAGCACCTACTCAATGTCCAGTACAGGATGCATCCATCCATCAGTTTATTTCCAAAAAGGGAGTTCTACGACAACCAGATAGTAGATGGTCCAAATGTCAAAGAAAAAAGCTATGAGAGGTGCTTCCTCAAGGGAAAAATGTACCAATCCTATTCCTTCATAAATGTTGCCAATGGAAAAGAAGAATTGGATCACAGATTTAGTCGGAAAAATATGGTGGAGGTTGCTGTAGTCTCTGGGATAGTAGCAAGCCTTTATAAAGAATTCATTGGGACAAAGCAGAAAGTTAGTATTGGGGTCATATCACCATACAAGGCTCAAGTTTATGCAATTCAAGAGATACTCAGAAAATATACTGAAACTTCTCACACTGGCTTCTCTCTAAGTGTGCGAACTGTTGATGGGTTCCAAGGTGGTGAAGAAGATGTGATAATTATATCTACTGTCCGATGTAATGGGAAGGGGTCAGTTGGTTTCTTGTCGAACCAGCAAAGAGCAAATGTTGCCCTAACACGTGCAAGGTATTGCCTTTGGATATTGGGGAATTCTTCAACTTTGATTAGTAGTGACTCTGTTTGGAAGAAGCTAGTCCTTGATGCCAAGAGACGGAATTGTTTTTATAATGCTGATGAAGACAGCAACTTGGCTCAGGCTATTACAGCTGCCCTGCTGGAGCTTGACCAACTTCATTCTCTGCTTAATATCGACTCTATGCTGTTCAAAAATGCTATATGGAAGGTTTGCTTCACTCACAACTTTTTGAGCTCCATAACAAAAATTAAAGACACTGTGATTCTTTGGGAAGTGCTTGCGTTATTAACCAAGCTTTCGAGTGGATGGCGCCGACCTCTTGAGGAAAAAGGAACTTTAGTGTATGATGGGACTTCTGCTCAACTGTTAGAGAAGTATAAAATCAATGGGAACTTGAATCTCATTTGGACTGTAGATATTCTCCAGGAGAATGGACATTACATCCAAGTTATGAAGTTTTGGGATATTTTGCCATTTTCTCATATAGCAGAACTAGCAAAGCGTCTTGACATTGTTTTTGGGAATTTTACAGTGGACAAGATGAACCGCTGCCGTCACAAATGCATTGACAGGGATGTTGTTGTTCCAATGAGATGGCCGGTGGTTTTCAGCAATTCCCCTATGGCTGATCATGAGGAGTCCCTTTCAAAACCATTGTCTTCTTTCAGTATAACAACTAATCGAGAAACAGCAACTTCAACATATGGGAATACATCGAAAGCAGTAAAACCAATTATCCCTTCGAAAAGCAATGTCAATCAAAGAGAAAGATTGCTATGGAAAATAAAAGCTGAAAGAGTGAAGGATTTCAGCACTTGCCCTGAAGCTAACCCTGTGGACTTGTCAAAACCATTATCATCACTAAGTCTAGCAGATAAGCCAGAAGCATCTACATCCACTGACATGGAGACAGGGAAAGCAGTAAAACCAATTATCCCTTCGAAAAGCAATGTCAATAAAAAAAAAAGATTGTTATGGAAAATAAAGGGTGAAGAAGGAGTGAAGGATTTCAGCACTTGCCTTGAAGCTAACCCTGTGGACTTGTCAAAACCATTATCATCACTAAGTCTAGCAGATAAGCCAGAAGCATCTACTTCCGTTGACATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12

Protein Analysis

1191

Amino Acids

134.16

Weight (kDa)

8.58

Isoelectric Point (pI)

36.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 80 - 212 1.1e-13 Domain of unknown function (DUF6469)
AAA_11 PF13086 263 - 624 2.4e-33 AAA domain
AAA_12 PF13087 632 - 828 1.1e-56 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000279)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65810 AT1G65810 AT1G65810 AT4G05540 AT5G37140 AT5G37150 AT5G37160 AT5G37165 AT5G52090
fragaria_vesca FvH4_1g27541 FvH4_1g27542 FvH4_2g08960 FvH4_2g08960 FvH4_2g08960 FvH4_2g08960 FvH4_3g00170 FvH4_3g00170 FvH4_3g00170 FvH4_5g05930 FvH4_5g05930
malus_domestica MD08G1077700.v1.1 MD08G1077900.v1.1 MD08G1078000.v1.1 MD08G1078100.v1.1 MD08G1078300.v1.1 MD09G1057800.v1.1 MD09G1281400.v1.1 MD11G1222900.v1.1 MD14G1146400.v1.1
prunus_persica Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G202100_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.3G013100_v2.0.a1
pyrus_communis pycom09g18900 pycom09g18910 pycom13g26720 pycom13g26730 pycom13g26760 pycom13g26770
rosa_chinensis RchiOBHm_Chr3g0488001 RchiOBHm_Chr3g0488011 RchiOBHm_Chr3g0488031 RchiOBHm_Chr3g0488061 RchiOBHm_Chr3g0488111 RchiOBHm_Chr3g0488201 RchiOBHm_Chr3g0488211 RchiOBHm_Chr3g0488221 RchiOBHm_Chr3g0488321 RchiOBHm_Chr3g0488431 RchiOBHm_Chr6g0264601 RchiOBHm_Chr6g0264641
rosa_laevigata RLG00000014240 RLG00000014241 RLG00000022910 RLG00000022949 RLG00000030376
rosa_multiflora Rmu_co8075388.1_g000001 Rmu_co8424869.1_g000001 Rmu_co8427783.1_g000001 Rmu_sc0000546.1_g000002 Rmu_sc0000546.1_g000015 Rmu_sc0011052.1_g000014 Rmu_sc0017772.1_g000004
rosa_roxburghii Rroxscaffold_175G00431980 Rroxscaffold_175G00431990 Rroxscaffold_1G00034600 Rroxscaffold_6G00394230 Rroxscaffold_6G00394270 Rroxscaffold_6G00394280 Rroxscaffold_6G00394350 Rroxscaffold_7G00203240 Rroxscaffold_7G00203270 Rroxscaffold_7G00203280 Rroxscaffold_7G00203300 Rroxscaffold_7G00205740
rosa_rugosa Rorug03G0238000 Rorug03G0238100 Rorug03G0238100 Rorug03G0238500 Rorug05G0155400 Rorug05G0356000 Rorug05G0463000 Rorug06G0015200 Rorug06G0015300 Rorug06G0015400 Rorug06G0015500 Rorug06G0015500
rosa_samantha Rh1DG088100 Rh2AG244800 Rh2BG187500 Rh2CG247300 Rh3BG322700 Rh3BG322800 Rh3BG323100 Rh3BG323900 Rh3BG324000 Rh3BG324300 Rh3CG321300 Rh3CG321400 Rh3CG321500 Rh3DG319400 Rh3DG319900 Rh3DG320100 Rh3DG320200 Rh6AG133700 Rh6AG133800 Rh6AG133900 Rh6CG129300 Rh6CG129800 Rh6CG130200 Rh6DG116500 Rh6DG117200 Rh7DG378000
rosa_wichuraiana Rw2G014710 Rw3G025340 Rw3G025350 Rw3G025360 Rw5G020630 Rw6G011590 Rw6G011600 Rw7G041380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2193, 2556
Acc36I ACCTGC 1 cut(s) 1023
AccB1I GGYRCC 1 cut(s) 2775
AccB7I CCANNNNNTGG 1 cut(s) 365
AccIII TCCGGA 1 cut(s) 999
AciI CCGC 2 cut(s) 1816, 3013
AclWI GGATC 4 cut(s) 121, 134, 1202, 2133
AcoI YGGCCR 1 cut(s) 3062
AcsI RAATTY 9 cut(s) 249, 476, 853, 1349, 1390, 1616, 2198, 2479, 2989
AcuI CTGAAG 2 cut(s) 1394, 3309
AcvI CACGTG 1 cut(s) 2450
AcyI GRCGYC 1 cut(s) 2776
AfaI GTAC 6 cut(s) 886, 1397, 1588, 1690, 1958, 2078
AfiI CCNNNNNNNGG 6 cut(s) 365, 887, 1006, 1163, 1858, 2541
AflIII ACRYGT 3 cut(s) 953, 2447, 3570
AhdI GACNNNNNGTC 1 cut(s) 906
AhlI ACTAGT 1 cut(s) 31
AjnI CCWGG 3 cut(s) 280, 880, 2889
AjuI GAANNNNNNNTTGG 4 cut(s) 3190, 3222, 3388, 3420
AleI CACNNNNGTG 1 cut(s) 140
AloI GAACNNNNNNTCC 2 cut(s) 1038, 1070
Alw21I GWGCWC 2 cut(s) 961, 2702
Alw26I GTCTC 4 cut(s) 1817, 2173, 2533, 3368
AlwI GGATC 4 cut(s) 121, 134, 1202, 2133
AlwNI CAGNNNCTG 2 cut(s) 326, 1372
Ama87I CYCGRG 1 cut(s) 1022
Aor13HI TCCGGA 1 cut(s) 999
AoxI GGCC 3 cut(s) 422, 767, 3062
ApeKI GCWGC 6 cut(s) 48, 809, 1763, 1877, 2597, 3015
ApoI RAATTY 9 cut(s) 249, 476, 853, 1349, 1390, 1616, 2198, 2479, 2989
ArsI GACNNNNNNTTYG 4 cut(s) 2966, 2998, 3107, 3139
AseI ATTAAT 1 cut(s) 866
Asp700I GAANNNNTTC 3 cut(s) 167, 3272, 3473
AspLEI GCGC 1 cut(s) 2778
AspS9I GGNCC 5 cut(s) 275, 344, 875, 1153, 2024
AsuC2I CCSGG 1 cut(s) 706
AsuHPI GGTGA 6 cut(s) 296, 839, 1447, 2232, 2363, 3467
AsuII TTCGAA 2 cut(s) 3209, 3407
AvaI CYCGRG 1 cut(s) 1022
AvaII GGWCC 5 cut(s) 275, 344, 875, 1153, 2024
AxyI CCTNAGG 1 cut(s) 1212
BamHI GGATCC 1 cut(s) 126
BanI GGYRCC 1 cut(s) 2775
BanII GRGCYC 1 cut(s) 2702
BbrPI CACGTG 1 cut(s) 2450
BbsI GAAGAC 5 cut(s) 227, 905, 1918, 2574, 3117
Bbv12I GWGCWC 2 cut(s) 961, 2702
BbvI GCAGC 6 cut(s) 60, 796, 1750, 1889, 2584, 3002
BccI CCATC 8 cut(s) 671, 1978, 1982, 2015, 2330, 2766, 2807, 3054
BceAI ACGGC 1 cut(s) 3003
BcgI CGANNNNNNTGC 2 cut(s) 1478, 1512
BciT130I CCWGG 3 cut(s) 282, 882, 2891
BciVI GTATCC 1 cut(s) 1922
BclI TGATCA 2 cut(s) 1120, 3094
BcnI CCSGG 1 cut(s) 706
BcoDI GTCTC 4 cut(s) 1817, 2173, 2533, 3368
BcuI ACTAGT 1 cut(s) 31
BfmI CTRYAG 3 cut(s) 810, 2163, 2877
BfoI RGCGCY 1 cut(s) 2779
BfuAI ACCTGC 1 cut(s) 1023
BfuI GTATCC 1 cut(s) 1922
BisI GCNGC 6 cut(s) 49, 810, 1764, 1878, 2598, 3016
BlpI GCTNAGC 1 cut(s) 1767
BlsI GCNGC 6 cut(s) 50, 811, 1765, 1879, 2599, 3017
Bme1390I CCNGG 4 cut(s) 282, 706, 882, 2891
Bme18I GGWCC 5 cut(s) 275, 344, 875, 1153, 2024
BmeRI GACNNNNNGTC 1 cut(s) 906
BmeT110I CYCGRG 1 cut(s) 1022
BmgT120I GGNCC 5 cut(s) 275, 344, 875, 1153, 2024
BmiI GGNNCC 5 cut(s) 128, 876, 1155, 2342, 2777
BmrFI CCNGG 4 cut(s) 282, 706, 882, 2891
BmsI GCATC 6 cut(s) 1703, 1953, 1975, 2521, 3362, 3563
BpiI GAAGAC 5 cut(s) 227, 905, 1918, 2574, 3117
BpmI CTGGAG 3 cut(s) 476, 2627, 2873
Bpu10I CCTNAGC 1 cut(s) 2583
Bpu1102I GCTNAGC 1 cut(s) 1767
Bpu14I TTCGAA 2 cut(s) 3209, 3407
BpuEI CTTGAG 3 cut(s) 2048, 2238, 2807
BpuMI CCSGG 1 cut(s) 706
BsaAI YACGTR 1 cut(s) 2450
BsaBI GATNNNNATC 1 cut(s) 333
BsaHI GRCGYC 1 cut(s) 2776
BsaI GGTCTC 1 cut(s) 1817
BsaJI CCNNGG 3 cut(s) 428, 705, 2344
BsaWI WCCGGW 1 cut(s) 999
BsaXI ACNNNNNCTCC 4 cut(s) 943, 973, 1038, 1068
Bsc4I CCNNNNNNNGG 6 cut(s) 365, 887, 1006, 1163, 1858, 2541
Bse118I RCCGGY 1 cut(s) 3064
Bse1I ACTGG 6 cut(s) 493, 892, 1015, 1920, 1954, 2311
Bse21I CCTNAGG 1 cut(s) 1212
Bse3DI GCAATG 6 cut(s) 375, 751, 1484, 1863, 3223, 3421
Bse8I GATNNNNATC 1 cut(s) 333
BseAI TCCGGA 1 cut(s) 999
BseBI CCWGG 3 cut(s) 282, 882, 2891
BseDI CCNNGG 3 cut(s) 428, 705, 2344
BseGI GGATG 8 cut(s) 91, 1966, 1968, 1970, 2777, 2907, 3046, 3359
BseJI GATNNNNATC 1 cut(s) 333
BseLI CCNNNNNNNGG 6 cut(s) 365, 887, 1006, 1163, 1858, 2541
BseMI GCAATG 6 cut(s) 375, 751, 1484, 1863, 3223, 3421
BseMII CTCAG 5 cut(s) 639, 1203, 1781, 2293, 2597
BseNI ACTGG 6 cut(s) 493, 892, 1015, 1920, 1954, 2311
BseRI GAGGAG 2 cut(s) 1295, 3116
BseXI GCAGC 6 cut(s) 60, 796, 1750, 1889, 2584, 3002
BsgI GTGCAG 2 cut(s) 140, 961
BshFI GGCC 3 cut(s) 424, 769, 3064
BshNI GGYRCC 1 cut(s) 2775
BsiHKAI GWGCWC 2 cut(s) 961, 2702
BsiHKCI CYCGRG 1 cut(s) 1022
BsiSI CCGG 3 cut(s) 705, 1000, 3065
BslFI GGGAC 4 cut(s) 1139, 2221, 2830, 3091
BslI CCNNNNNNNGG 6 cut(s) 365, 887, 1006, 1163, 1858, 2541
BsmAI GTCTC 4 cut(s) 1817, 2173, 2533, 3368
BsmBI CGTCTC 1 cut(s) 2533
BsmFI GGGAC 4 cut(s) 1139, 2221, 2830, 3091
BsmI GAATGC 2 cut(s) 1270, 1489
BsnI GGCC 3 cut(s) 424, 769, 3064
Bso31I GGTCTC 1 cut(s) 1817
BsoBI CYCGRG 1 cut(s) 1022
Bsp119I TTCGAA 2 cut(s) 3209, 3407
Bsp1286I GDGCHC 2 cut(s) 961, 2702
Bsp13I TCCGGA 1 cut(s) 999
Bsp1407I TGTACA 2 cut(s) 1395, 1586
Bsp143I GATC 8 cut(s) 126, 487, 1099, 1120, 1207, 1359, 2125, 3094
Bsp1720I GCTNAGC 1 cut(s) 1767
BspACI CCGC 2 cut(s) 1816, 3013
BspANI GGCC 3 cut(s) 424, 769, 3064
BspCNI CTCAG 5 cut(s) 638, 1204, 1780, 2292, 2596
BspEI TCCGGA 1 cut(s) 999
BspHI TCATGA 1 cut(s) 3097
BspLI GGNNCC 5 cut(s) 128, 876, 1155, 2342, 2777
BspMAI CTGCAG 1 cut(s) 814
BspMI ACCTGC 1 cut(s) 1023
BspPI GGATC 4 cut(s) 121, 134, 1202, 2133
BspT104I TTCGAA 2 cut(s) 3209, 3407
BspT107I GGYRCC 1 cut(s) 2775
BspTNI GGTCTC 1 cut(s) 1817
BsrDI GCAATG 6 cut(s) 375, 751, 1484, 1863, 3223, 3421
BsrFI RCCGGY 1 cut(s) 3064
BsrGI TGTACA 2 cut(s) 1395, 1586
BsrI ACTGG 6 cut(s) 493, 892, 1015, 1920, 1954, 2311
BssAI RCCGGY 1 cut(s) 3064
BssECI CCNNGG 3 cut(s) 428, 705, 2344
BssMI GATC 8 cut(s) 126, 487, 1099, 1120, 1207, 1359, 2125, 3094
BssNI GRCGYC 1 cut(s) 2776
BssT1I CCWWGG 2 cut(s) 428, 2344
Bst2UI CCWGG 3 cut(s) 282, 882, 2891
Bst4CI ACNGT 8 cut(s) 824, 1552, 2332, 2380, 2725, 2832, 2878, 2998
Bst6I CTCTTC 1 cut(s) 5
BstACI GRCGYC 1 cut(s) 2776
BstAPI GCANNNNNTGC 2 cut(s) 1883, 2438
BstAUI TGTACA 2 cut(s) 1395, 1586
BstBAI YACGTR 1 cut(s) 2450
BstBI TTCGAA 2 cut(s) 3209, 3407
BstC8I GCNNGC 4 cut(s) 751, 1675, 2185, 2745
BstDEI CTNAG 8 cut(s) 625, 1212, 1767, 2279, 2318, 2583, 3329, 3530
BstF5I GGATG 8 cut(s) 91, 1966, 1968, 1970, 2777, 2907, 3046, 3359
BstH2I RGCGCY 1 cut(s) 2779
BstHHI GCGC 1 cut(s) 2778
BstKTI GATC 8 cut(s) 129, 490, 1102, 1123, 1210, 1362, 2128, 3097
BstMAI GTCTC 4 cut(s) 1817, 2173, 2533, 3368
BstMBI GATC 8 cut(s) 126, 487, 1099, 1120, 1207, 1359, 2125, 3094
BstMWI GCNNNNNNNGC 5 cut(s) 750, 1883, 2438, 2594, 2960
BstNI CCWGG 3 cut(s) 282, 882, 2891
BstNSI RCATGY 5 cut(s) 236, 544, 957, 1718, 3574
BstSCI CCNGG 4 cut(s) 280, 704, 880, 2889
BstSFI CTRYAG 3 cut(s) 810, 2163, 2877
BstV1I GCAGC 6 cut(s) 60, 796, 1750, 1889, 2584, 3002
BstV2I GAAGAC 5 cut(s) 227, 905, 1918, 2574, 3117
BstX2I RGATCY 1 cut(s) 126
BstXI CCANNNNNNTGG 3 cut(s) 2897, 3060, 3370
BstYI RGATCY 1 cut(s) 126
Bsu36I CCTNAGG 1 cut(s) 1212
BsuI GTATCC 1 cut(s) 1922
BsuRI GGCC 3 cut(s) 424, 769, 3064
BtsCI GGATG 8 cut(s) 91, 1966, 1968, 1970, 2777, 2907, 3046, 3359
BtsI GCAGTG 1 cut(s) 981
BtsIMutI CAGTG 5 cut(s) 981, 2304, 2721, 3003, 3363
BveI ACCTGC 1 cut(s) 1023
Cac8I GCNNGC 4 cut(s) 751, 1675, 2185, 2745
CaiI CAGNNNCTG 2 cut(s) 326, 1372
CciI TCATGA 1 cut(s) 3097
CfoI GCGC 1 cut(s) 2778
Cfr10I RCCGGY 1 cut(s) 3064
Cfr13I GGNCC 5 cut(s) 275, 344, 875, 1153, 2024
CseI GACGC 1 cut(s) 2957
Csp6I GTAC 6 cut(s) 885, 1396, 1587, 1689, 1957, 2077
CspCI CAANNNNNGTGG 2 cut(s) 952, 987
CviQI GTAC 6 cut(s) 885, 1396, 1587, 1689, 1957, 2077
DdeI CTNAG 8 cut(s) 625, 1212, 1767, 2279, 2318, 2583, 3329, 3530
DinI GGCGCC 1 cut(s) 2777
DpnI GATC 8 cut(s) 128, 489, 1101, 1122, 1209, 1361, 2127, 3096
DpnII GATC 8 cut(s) 126, 487, 1099, 1120, 1207, 1359, 2125, 3094
DriI GACNNNNNGTC 1 cut(s) 906
EaeI YGGCCR 1 cut(s) 3062
Eam1104I CTCTTC 1 cut(s) 5
Eam1105I GACNNNNNGTC 1 cut(s) 906
EarI CTCTTC 1 cut(s) 5
EciI GGCGGA 1 cut(s) 1805
Ecl136II GAGCTC 1 cut(s) 2700
Eco130I CCWWGG 2 cut(s) 428, 2344
Eco147I AGGCCT 1 cut(s) 424
Eco24I GRGCYC 1 cut(s) 2702
Eco31I GGTCTC 1 cut(s) 1817
Eco32I GATATC 1 cut(s) 373
Eco47I GGWCC 5 cut(s) 275, 344, 875, 1153, 2024
Eco53kI GAGCTC 1 cut(s) 2700
Eco57I CTGAAG 2 cut(s) 1394, 3309
Eco72I CACGTG 1 cut(s) 2450
Eco81I CCTNAGG 1 cut(s) 1212
Eco88I CYCGRG 1 cut(s) 1022
EcoICRI GAGCTC 1 cut(s) 2700
EcoO109I RGGNCCY 1 cut(s) 875
EcoRI GAATTC 4 cut(s) 853, 1616, 2198, 2479
EcoRII CCWGG 3 cut(s) 280, 880, 2889
EcoRV GATATC 1 cut(s) 373
EcoT14I CCWWGG 2 cut(s) 428, 2344
EcoT22I ATGCAT 5 cut(s) 407, 546, 1648, 1968, 3032
EcoT38I GRGCYC 1 cut(s) 2702
EgeI GGCGCC 1 cut(s) 2777
EheI GGCGCC 1 cut(s) 2777
ErhI CCWWGG 2 cut(s) 428, 2344
Esp3I CGTCTC 1 cut(s) 2533
FalI AAGNNNNNCTT 2 cut(s) 2663, 2695
FaqI GGGAC 4 cut(s) 1139, 2221, 2830, 3091
FauNDI CATATG 1 cut(s) 3169
FbaI TGATCA 2 cut(s) 1120, 3094
Fnu4HI GCNGC 6 cut(s) 49, 810, 1764, 1878, 2598, 3016
FokI GGATG 8 cut(s) 98, 1953, 1957, 1975, 2784, 2894, 3053, 3346
FriOI GRGCYC 1 cut(s) 2702
Fsp4HI GCNGC 6 cut(s) 49, 810, 1764, 1878, 2598, 3016
GlaI GCGC 1 cut(s) 2777
GluI GCNGC 6 cut(s) 49, 810, 1764, 1878, 2598, 3016
GsuI CTGGAG 3 cut(s) 476, 2627, 2873
HaeII RGCGCY 1 cut(s) 2779
HaeIII GGCC 3 cut(s) 424, 769, 3064
HapII CCGG 3 cut(s) 705, 1000, 3065
HgaI GACGC 1 cut(s) 2957
HhaI GCGC 1 cut(s) 2778
Hin1I GRCGYC 1 cut(s) 2776
Hin6I GCGC 1 cut(s) 2776
HinP1I GCGC 1 cut(s) 2776
HincII GTYRAC 2 cut(s) 1012, 3568
HindII GTYRAC 2 cut(s) 1012, 3568
HindIII AAGCTT 1 cut(s) 2759
HpaI GTTAAC 1 cut(s) 1012
HpaII CCGG 3 cut(s) 705, 1000, 3065
HphI GGTGA 6 cut(s) 296, 839, 1447, 2232, 2363, 3467
Hpy166II GTNNAC 7 cut(s) 1012, 1398, 1587, 3001, 3304, 3505, 3568
Hpy188I TCNGA 9 cut(s) 85, 255, 628, 673, 912, 1451, 2142, 2282, 2384
Hpy8I GTNNAC 7 cut(s) 1012, 1398, 1587, 3001, 3304, 3505, 3568
Hpy99I CGWCG 1 cut(s) 1563
HpyCH4III ACNGT 8 cut(s) 824, 1552, 2332, 2380, 2725, 2832, 2878, 2998
HpyCH4IV ACGT 2 cut(s) 305, 2449
HpyF10VI GCNNNNNNNGC 5 cut(s) 750, 1883, 2438, 2594, 2960
HpyF3I CTNAG 8 cut(s) 625, 1212, 1767, 2279, 2318, 2583, 3329, 3530
HpySE526I ACGT 2 cut(s) 305, 2449
Hsp92I GRCGYC 1 cut(s) 2776
HspAI GCGC 1 cut(s) 2776
KasI GGCGCC 1 cut(s) 2775
Kpn2I TCCGGA 1 cut(s) 999
Ksp22I TGATCA 2 cut(s) 1120, 3094
KspAI GTTAAC 1 cut(s) 1012
Kzo9I GATC 8 cut(s) 126, 487, 1099, 1120, 1207, 1359, 2125, 3094
LmnI GCTCC 4 cut(s) 964, 1369, 2608, 2705
Lsp1109I GCAGC 6 cut(s) 60, 796, 1750, 1889, 2584, 3002
LweI GCATC 6 cut(s) 1703, 1953, 1975, 2521, 3362, 3563
MaeII ACGT 2 cut(s) 305, 2449
MalI GATC 8 cut(s) 128, 489, 1101, 1122, 1209, 1361, 2127, 3096
MboI GATC 8 cut(s) 126, 487, 1099, 1120, 1207, 1359, 2125, 3094
MfeI CAATTG 1 cut(s) 258
MflI RGATCY 1 cut(s) 126
MhlI GDGCHC 2 cut(s) 961, 2702
Mly113I GGCGCC 1 cut(s) 2776
MlyI GAGTC 6 cut(s) 217, 916, 990, 2498, 2636, 3113
MmeI TCCRAC 2 cut(s) 346, 2120
Mph1103I ATGCAT 5 cut(s) 407, 546, 1648, 1968, 3032
MroI TCCGGA 1 cut(s) 999
MroXI GAANNNNTTC 3 cut(s) 167, 3272, 3473
MslI CAYNNNNRTG 2 cut(s) 140, 3368
MspA1I CMGCKG 3 cut(s) 272, 2597, 3015
MspI CCGG 3 cut(s) 705, 1000, 3065
MspR9I CCNGG 4 cut(s) 282, 706, 882, 2891
MunI CAATTG 1 cut(s) 258
Mva1269I GAATGC 2 cut(s) 1270, 1489
MvaI CCWGG 3 cut(s) 282, 882, 2891
MwoI GCNNNNNNNGC 5 cut(s) 750, 1883, 2438, 2594, 2960
NarI GGCGCC 1 cut(s) 2776
NciI CCSGG 1 cut(s) 706
NdeI CATATG 1 cut(s) 3169
NdeII GATC 8 cut(s) 126, 487, 1099, 1120, 1207, 1359, 2125, 3094
NlaIV GGNNCC 5 cut(s) 128, 876, 1155, 2342, 2777
NmuCI GTSAC 7 cut(s) 142, 228, 284, 1017, 1106, 2501, 3020
NsiI ATGCAT 5 cut(s) 407, 546, 1648, 1968, 3032
NspI RCATGY 5 cut(s) 236, 544, 957, 1718, 3574
NspV TTCGAA 2 cut(s) 3209, 3407
OliI CACNNNNGTG 1 cut(s) 140
PaeR7I CTCGAG 1 cut(s) 1022
PagI TCATGA 1 cut(s) 3097
PceI AGGCCT 1 cut(s) 424
PciI ACATGT 2 cut(s) 953, 3570
PctI GAATGC 2 cut(s) 1270, 1489
PdmI GAANNNNTTC 3 cut(s) 167, 3272, 3473
PflFI GACNNNGTC 2 cut(s) 226, 1445
PflMI CCANNNNNTGG 1 cut(s) 365
PfoI TCCNGGA 1 cut(s) 2889
PkrI GCNGC 6 cut(s) 50, 811, 1765, 1879, 2599, 3017
PleI GAGTC 6 cut(s) 217, 915, 990, 2498, 2636, 3112
PluTI GGCGCC 1 cut(s) 2779
PmaCI CACGTG 1 cut(s) 2450
PmlI CACGTG 1 cut(s) 2450
PpsI GAGTC 6 cut(s) 217, 915, 990, 2498, 2636, 3112
Ppu21I YACGTR 1 cut(s) 2450
PpuMI RGGWCCY 1 cut(s) 875
PscI ACATGT 2 cut(s) 953, 3570
PshBI ATTAAT 1 cut(s) 866
PsiI TTATAA 2 cut(s) 2193, 2556
Psp124BI GAGCTC 1 cut(s) 2702
Psp5II RGGWCCY 1 cut(s) 875
Psp6I CCWGG 3 cut(s) 280, 880, 2889
PspCI CACGTG 1 cut(s) 2450
PspGI CCWGG 3 cut(s) 280, 880, 2889
PspN4I GGNNCC 5 cut(s) 128, 876, 1155, 2342, 2777
PspPI GGNCC 5 cut(s) 275, 344, 875, 1153, 2024
PspPPI RGGWCCY 1 cut(s) 875
PspXI VCTCGAGB 1 cut(s) 1022
PsrI GAACNNNNNNTAC 2 cut(s) 1570, 1602
PstI CTGCAG 1 cut(s) 814
PstNI CAGNNNCTG 2 cut(s) 326, 1372
PsuI RGATCY 1 cut(s) 126
PsyI GACNNNGTC 2 cut(s) 226, 1445
PvuII CAGCTG 2 cut(s) 272, 2597
RsaI GTAC 6 cut(s) 886, 1397, 1588, 1690, 1958, 2078
RsaNI GTAC 6 cut(s) 885, 1396, 1587, 1689, 1957, 2077
RseI CAYNNNNRTG 2 cut(s) 140, 3368
SacI GAGCTC 1 cut(s) 2702
SatI GCNGC 6 cut(s) 49, 810, 1764, 1878, 2598, 3016
Sau3AI GATC 8 cut(s) 126, 487, 1099, 1120, 1207, 1359, 2125, 3094
Sau96I GGNCC 5 cut(s) 275, 344, 875, 1153, 2024
SchI GAGTC 6 cut(s) 217, 916, 990, 2498, 2636, 3113
ScrFI CCNGG 4 cut(s) 282, 706, 882, 2891
SduI GDGCHC 2 cut(s) 961, 2702
SfaNI GCATC 6 cut(s) 1703, 1953, 1975, 2521, 3362, 3563
SfcI CTRYAG 3 cut(s) 810, 2163, 2877
SfoI GGCGCC 1 cut(s) 2777
Sfr274I CTCGAG 1 cut(s) 1022
SfuI TTCGAA 2 cut(s) 3209, 3407
SinI GGWCC 5 cut(s) 275, 344, 875, 1153, 2024
SlaI CTCGAG 1 cut(s) 1022
SmiMI CAYNNNNRTG 2 cut(s) 140, 3368
SmlI CTYRAG 4 cut(s) 1022, 2063, 2253, 2786
SmoI CTYRAG 4 cut(s) 1022, 2063, 2253, 2786
SpeI ACTAGT 1 cut(s) 31
SseBI AGGCCT 1 cut(s) 424
SsiI CCGC 2 cut(s) 1816, 3013
SspDI GGCGCC 1 cut(s) 2775
SspI AATATT 3 cut(s) 970, 1223, 1683
SstI GAGCTC 1 cut(s) 2702
StuI AGGCCT 1 cut(s) 424
StyD4I CCNGG 4 cut(s) 280, 704, 880, 2889
StyI CCWWGG 2 cut(s) 428, 2344
TaaI ACNGT 8 cut(s) 824, 1552, 2332, 2380, 2725, 2832, 2878, 2998
TaiI ACGT 2 cut(s) 308, 2452
TaqII GACCGA 1 cut(s) 361
TatI WGTACW 4 cut(s) 1395, 1586, 1688, 1956
TscAI CASTG 5 cut(s) 981, 2311, 2728, 3003, 3370
TseFI GTSAC 7 cut(s) 142, 228, 284, 1017, 1106, 2501, 3020
TseI GCWGC 6 cut(s) 48, 809, 1763, 1877, 2597, 3015
Tsp45I GTSAC 7 cut(s) 142, 228, 284, 1017, 1106, 2501, 3020
TspGWI ACGGA 3 cut(s) 593, 2557, 3553
TspRI CASTG 5 cut(s) 981, 2311, 2728, 3003, 3370
Tth111I GACNNNGTC 2 cut(s) 226, 1445
Van91I CCANNNNNTGG 1 cut(s) 365
VpaK11BI GGWCC 5 cut(s) 275, 344, 875, 1153, 2024
VspI ATTAAT 1 cut(s) 866
XapI RAATTY 9 cut(s) 249, 476, 853, 1349, 1390, 1616, 2198, 2479, 2989
XceI RCATGY 5 cut(s) 236, 544, 957, 1718, 3574
XcmI CCANNNNNNNNNTGG 1 cut(s) 2018
XhoI CTCGAG 1 cut(s) 1022
XmnI GAANNNNTTC 3 cut(s) 167, 3272, 3473
Zsp2I ATGCAT 5 cut(s) 407, 546, 1648, 1968, 3032
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.