FvH4_2g13451

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000291
Physical Location & Seq
Reverse (-)
11755328 .. 11755783
456 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g13451.t1

Sequence Viewer

Length: 456 bp
ATGAAGAAGTGTACTTTTTGCACCAATAAGCTGCTGTTTCTAGGCTTTGTGGTTGGAGAGGATGGGATTCAGGTTGATGAAGAGAAGGTACATGCAATTAGAGAGTGGCCAACTCCTAGAACAGTTTCGGAGGTGAGAAGTTTCCATGGTTTAGCTACTTTCTATAGCCGGTTTGTGAGGGATTTCAATACCATCACTACACCAATTACTGAATGCATGAAGAAAGGCAAGTTTCATTGGGGTGAAGAGCAAGAGCAAAGTTTTGCACTTATCAAAGAGAAGCTTTGTACCGCACCAGTTTTGGCTCTTCCAAATTTTGACAAGATATTCGAGGTCAAATGTGATGCTAGTGGTGTTGGAGTGGGGGCTGTCTTGTCCCAAGAAAAGAGGGCAGTGGCTTTCTTTAAGGCAATGGGAGCATTATCTGATCCAAAGAGAGTTTATTTTGTTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 Retrotrans_gag rve zf-CCHC zf-H2C2

Protein Analysis

152

Amino Acids

17.05

Weight (kDa)

8.6

Isoelectric Point (pI)

40.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RT_RNaseH_2 PF17919 80 - 130 1.5e-16 RNase H-like domain found in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000291)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG00860
fragaria_vesca FvH4_1g23712 FvH4_1g29809 FvH4_1g29810 FvH4_2g02661 FvH4_2g13451 FvH4_2g17921 FvH4_2g17922 FvH4_2g19251 FvH4_2g19252 FvH4_2g28221 FvH4_2g35961 FvH4_3g10372 FvH4_3g12461 FvH4_3g12461 FvH4_3g27861 FvH4_3g34591 FvH4_3g40201 FvH4_3g44612 FvH4_4g08352 FvH4_4g12251 FvH4_4g12742 FvH4_4g13904 FvH4_4g14287 FvH4_4g15243 FvH4_4g24071 FvH4_4g33448 FvH4_5g21912 FvH4_5g39371 FvH4_6g14992 FvH4_6g15221 FvH4_6g20603 FvH4_6g31093 FvH4_6g32372 FvH4_6g33012
malus_domestica MD11G1009100.v1.1 MD13G1145000.v1.1 MD16G1138700.v1.1
prunus_persica Prupe.5G246300_v2.0.a1 Prupe.7G034800_v2.0.a1 Prupe.8G084500_v2.0.a1
pyrus_communis pycom01g18240 pycom04g00330 pycom05g09010 pycom06g01670 pycom06g01690 pycom06g07180 pycom06g11620 pycom10g05720 pycom1134g00040 pycom1134g00060 pycom12486g00010 pycom12555g00230 pycom13g21670 pycom13g23130 pycom13g23140 pycom14g10580
rosa_chinensis RchiOBHm_Chr1g0330691 RchiOBHm_Chr2g0106171 RchiOBHm_Chr2g0134731 RchiOBHm_Chr2g0142791 RchiOBHm_Chr2g0149861 RchiOBHm_Chr3g0452401 RchiOBHm_Chr3g0461881 RchiOBHm_Chr4g0401341 RchiOBHm_Chr5g0075641 RchiOBHm_Chr6g0265961 RchiOBHm_Chr7g0200201 RchiOBHm_Chr7g0237811
rosa_laevigata RLG00000002773
rosa_multiflora Rmu_co8037806.1_g000001 Rmu_co8445989.1_g000001 Rmu_sc0000745.1_g000039 Rmu_sc0004448.1_g000029 Rmu_sc0004488.1_g000002 Rmu_sc0004591.1_g000018 Rmu_sc0005065.1_g000030 Rmu_sc0005150.1_g000005 Rmu_sc0005654.1_g000011 Rmu_sc0006986.1_g000016
rosa_roxburghii Rroxscaffold_164G00436490 Rroxscaffold_175G00432490 Rroxscaffold_1G00059440 Rroxscaffold_1G00064410 Rroxscaffold_2G00100310 Rroxscaffold_2G00128170 Rroxscaffold_3G00239420 Rroxscaffold_3G00264970 Rroxscaffold_3G00271550 Rroxscaffold_4G00300240 Rroxscaffold_5G00385050 Rroxscaffold_6G00399720
rosa_rugosa Rorug02G0252200
rosa_samantha Rh2DG251900 Rh7CG329900
rosa_wichuraiana Rw0G004800 Rw1G000850 Rw1G022350 Rw2G027030 Rw2G027760 Rw2G028410 Rw2G029130 Rw2G029390 Rw2G032490 Rw2G039460 Rw2G047800 Rw2G050340 Rw3G026430 Rw4G018370 Rw4G030750 Rw4G033140 Rw5G011950 Rw5G012010 Rw5G029280 Rw5G040190 Rw5G044570 Rw5G048240 Rw6G000340 Rw6G010140 Rw6G026480 Rw7G033750 Rw7G037250 Rw7G042080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 291
AclWI GGATC 1 cut(s) 422
AcoI YGGCCR 1 cut(s) 107
AcsI RAATTY 1 cut(s) 313
AfaI GTAC 3 cut(s) 13, 90, 289
AgsI TTSAA 1 cut(s) 187
AluBI AGCT 3 cut(s) 31, 155, 283
AluI AGCT 3 cut(s) 31, 155, 283
AlwI GGATC 1 cut(s) 422
AoxI GGCC 1 cut(s) 107
ApeKI GCWGC 1 cut(s) 31
ApoI RAATTY 1 cut(s) 313
ArsI GACNNNNNNTTYG 2 cut(s) 311, 343
Asp700I GAANNNNTTC 1 cut(s) 124
AsuHPI GGTGA 2 cut(s) 145, 254
BalI TGGCCA 1 cut(s) 109
BarI GAAGNNNNNNTAC 2 cut(s) 72, 104
BbvI GCAGC 1 cut(s) 18
BccI CCATC 2 cut(s) 56, 200
BfaI CTAG 3 cut(s) 41, 117, 348
BfmI CTRYAG 1 cut(s) 163
BisI GCNGC 1 cut(s) 32
BlsI GCNGC 1 cut(s) 33
BmsI GCATC 1 cut(s) 334
BsaJI CCNNGG 1 cut(s) 145
Bse118I RCCGGY 1 cut(s) 168
Bse1I ACTGG 1 cut(s) 296
Bse3DI GCAATG 1 cut(s) 417
BseDI CCNNGG 1 cut(s) 145
BseGI GGATG 1 cut(s) 67
BseMI GCAATG 1 cut(s) 417
BseNI ACTGG 1 cut(s) 296
BseXI GCAGC 1 cut(s) 18
BshFI GGCC 1 cut(s) 109
BsiSI CCGG 1 cut(s) 169
BslFI GGGAC 1 cut(s) 361
BsmFI GGGAC 1 cut(s) 361
BsmI GAATGC 1 cut(s) 218
BsnI GGCC 1 cut(s) 109
Bsp143I GATC 1 cut(s) 427
Bsp19I CCATGG 1 cut(s) 145
BspACI CCGC 1 cut(s) 291
BspANI GGCC 1 cut(s) 109
BspPI GGATC 1 cut(s) 422
BspQI GCTCTTC 2 cut(s) 240, 312
BsrDI GCAATG 1 cut(s) 417
BsrFI RCCGGY 1 cut(s) 168
BsrI ACTGG 1 cut(s) 296
BssAI RCCGGY 1 cut(s) 168
BssECI CCNNGG 1 cut(s) 145
BssMI GATC 1 cut(s) 427
BssT1I CCWWGG 1 cut(s) 145
Bst4CI ACNGT 1 cut(s) 124
Bst6I CTCTTC 3 cut(s) 75, 240, 312
BstDSI CCRYGG 1 cut(s) 145
BstF5I GGATG 1 cut(s) 67
BstKTI GATC 1 cut(s) 430
BstMBI GATC 1 cut(s) 427
BstMWI GCNNNNNNNGC 1 cut(s) 416
BstNSI RCATGY 1 cut(s) 95
BstSFI CTRYAG 1 cut(s) 163
BstV1I GCAGC 1 cut(s) 18
BsuRI GGCC 1 cut(s) 109
BtgI CCRYGG 1 cut(s) 145
BtsCI GGATG 1 cut(s) 67
BtsI GCAGTG 1 cut(s) 399
BtsIMutI CAGTG 1 cut(s) 399
Cfr10I RCCGGY 1 cut(s) 168
Csp6I GTAC 3 cut(s) 12, 89, 288
CviAII CATG 3 cut(s) 92, 146, 217
CviJI RGCY 9 cut(s) 31, 45, 109, 155, 168, 283, 305, 368, 398
CviKI_1 RGCY 9 cut(s) 31, 45, 109, 155, 168, 283, 305, 368, 398
CviQI GTAC 3 cut(s) 12, 89, 288
DpnI GATC 1 cut(s) 429
DpnII GATC 1 cut(s) 427
EaeI YGGCCR 1 cut(s) 107
Eam1104I CTCTTC 3 cut(s) 75, 240, 312
EarI CTCTTC 3 cut(s) 75, 240, 312
Eco130I CCWWGG 1 cut(s) 145
EcoT14I CCWWGG 1 cut(s) 145
EcoT22I ATGCAT 1 cut(s) 218
ErhI CCWWGG 1 cut(s) 145
FaeI CATG 3 cut(s) 95, 149, 220
FaiI YATR 4 cut(s) 93, 147, 165, 218
FalI AAGNNNNNCTT 2 cut(s) 267, 299
FaqI GGGAC 1 cut(s) 361
FatI CATG 3 cut(s) 91, 145, 216
Fnu4HI GCNGC 1 cut(s) 32
FokI GGATG 1 cut(s) 74
Fsp4HI GCNGC 1 cut(s) 32
FspBI CTAG 3 cut(s) 41, 117, 348
GluI GCNGC 1 cut(s) 32
HaeIII GGCC 1 cut(s) 109
HapII CCGG 1 cut(s) 169
Hin1II CATG 3 cut(s) 95, 149, 220
HindIII AAGCTT 1 cut(s) 281
HinfI GANTC 1 cut(s) 67
HpaII CCGG 1 cut(s) 169
HphI GGTGA 2 cut(s) 145, 254
Hpy166II GTNNAC 1 cut(s) 12
Hpy188I TCNGA 3 cut(s) 130, 427, 455
Hpy8I GTNNAC 1 cut(s) 12
HpyAV CCTTC 1 cut(s) 79
HpyCH4III ACNGT 1 cut(s) 124
HpyCH4V TGCA 4 cut(s) 21, 95, 216, 266
HpyF10VI GCNNNNNNNGC 1 cut(s) 416
Hsp92II CATG 3 cut(s) 95, 149, 220
Kzo9I GATC 1 cut(s) 427
LguI GCTCTTC 2 cut(s) 240, 312
LmnI GCTCC 1 cut(s) 416
LpnPI CCDG 3 cut(s) 56, 182, 309
Lsp1109I GCAGC 1 cut(s) 18
LweI GCATC 1 cut(s) 334
MaeI CTAG 3 cut(s) 41, 117, 348
MalI GATC 1 cut(s) 429
MboI GATC 1 cut(s) 427
MboII GAAGA 5 cut(s) 16, 92, 232, 257, 299
MlsI TGGCCA 1 cut(s) 109
MluCI AATT 3 cut(s) 96, 204, 313
MluNI TGGCCA 1 cut(s) 109
MmeI TCCRAC 2 cut(s) 34, 337
MnlI CCTC 5 cut(s) 52, 124, 171, 325, 381
Mox20I TGGCCA 1 cut(s) 109
Mph1103I ATGCAT 1 cut(s) 218
MroXI GAANNNNTTC 1 cut(s) 124
MscI TGGCCA 1 cut(s) 109
MseI TTAA 1 cut(s) 405
MslI CAYNNNNRTG 1 cut(s) 240
Msp20I TGGCCA 1 cut(s) 109
MspI CCGG 1 cut(s) 169
Mva1269I GAATGC 1 cut(s) 218
MwoI GCNNNNNNNGC 1 cut(s) 416
NcoI CCATGG 1 cut(s) 145
NdeII GATC 1 cut(s) 427
NlaIII CATG 3 cut(s) 95, 149, 220
NsiI ATGCAT 1 cut(s) 218
NspI RCATGY 1 cut(s) 95
PciSI GCTCTTC 2 cut(s) 240, 312
PctI GAATGC 1 cut(s) 218
PdmI GAANNNNTTC 1 cut(s) 124
PfeI GAWTC 1 cut(s) 67
PkrI GCNGC 1 cut(s) 33
RsaI GTAC 3 cut(s) 13, 90, 289
RsaNI GTAC 3 cut(s) 12, 89, 288
RseI CAYNNNNRTG 1 cut(s) 240
SapI GCTCTTC 2 cut(s) 240, 312
SaqAI TTAA 1 cut(s) 405
SatI GCNGC 1 cut(s) 32
Sau3AI GATC 1 cut(s) 427
SetI ASST 7 cut(s) 33, 75, 90, 135, 157, 285, 336
SfaNI GCATC 1 cut(s) 334
SfcI CTRYAG 1 cut(s) 163
SmiMI CAYNNNNRTG 1 cut(s) 240
Sse9I AATT 3 cut(s) 96, 204, 313
SsiI CCGC 1 cut(s) 291
SspMI CTAG 3 cut(s) 41, 117, 348
StyI CCWWGG 1 cut(s) 145
TaaI ACNGT 1 cut(s) 124
TaqI TCGA 1 cut(s) 330
TasI AATT 3 cut(s) 96, 204, 313
TatI WGTACW 1 cut(s) 11
TfiI GAWTC 1 cut(s) 67
Tru1I TTAA 1 cut(s) 405
Tru9I TTAA 1 cut(s) 405
TscAI CASTG 1 cut(s) 399
TseI GCWGC 1 cut(s) 31
TspDTI ATGAA 4 cut(s) 17, 93, 224, 233
TspRI CASTG 1 cut(s) 399
XapI RAATTY 1 cut(s) 313
XceI RCATGY 1 cut(s) 95
XmnI GAANNNNTTC 1 cut(s) 124
XspI CTAG 3 cut(s) 41, 117, 348
Zsp2I ATGCAT 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.