Rw1G000850

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000291
Physical Location & Seq
Forward (+)
1183289 .. 1187840
4552 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G000850.1

Sequence Viewer

Length: 3312 bp
ATGGCGGCCCAATTTGAGCAACAGTTAGATCGTGCTAAAGGAGGAAAACGAAAAGTGACTACACTTACAGCAGCGTTTACCCCTGCCATCAATTCCATGAATAATCAGATTGGAGAAATTCGTGGATTGTTGGGTGAGAGGAACAACAACAACAACAACAACAATAATCGGCGTAGAGATGGGGAAGGAGGCCAGCGAGTTAGGGCTCCACGTGGTGAAGTTGTTGATAATACATCAGAATCTGAGCCTGAAGAAGAAATTTTACAACAAGAGCAACATGGACAAGCTGACCAGGATTACAAAGTCAAGGCCGAAATTCCTTTCTTTTCGGGCAATTTGGGTGTAGAAGATTATTTGGATTGGCAGCTTCAGGTGGACAGATTCTTTGAGATTATGGAAGTGCCTAAACACAAGCAAGTTAAGCATGTTGCCTGGAGATTGAAGAGTACTGCTGCAGTATGGTGGGATAAGTTGCAGAACACTCGAAAGAAGCAGAGGAAGCAGGGTGTTAGAACATGGCGAAGAATGAAGCAGTTGATGATGGAGAGGTTCTTGCCAGATGATTATGAGCAGATTCTATATAGGCTGTTTATTGAATGTGTCCAGGGAACTAGGACGGTGGCTGATTATACGACTGAGTTCTTACGCTATTCAGAGCGTAATGAATTGGGAGAAACTGAAGGCCAGAAGGTGGCTCGCTATATCAGTGGGCTGAAGCCTTCCATTCAGGAGAGAATTGGGTTACAAACTGTTCATACTATGGCCGAAGTGACAAACCTAGCATTGAAAGCAGAGTTGTTGGAGAAACCTTCACGAGCTTCTTCTTTCCAGAGGTATAACTACCAAAGGAGTGCAGATTTGGTTAATGCCTCAACTGCCAAGGGTACAACTACGCTGCAGAGAGCAGAAAACGCCTTTAAAGCTTCTAATCCTCCTTTTAATGGCGCGGGCAGTTCTAACAATCCTAGTGGTGTTCAAAATAGAGCCCCGAATCAGAGGTTCAATAATCCTTATGCTAGACCTACAAGAGATGTCTGTTATCGATGCAACAAGCCTGGGCATAGATCTAATGTGTGTCCTGAGAGGAGACAAACTGCCCTTATAGATGATTATGATGAGGATGAAGAAGAAGTTAGGAGAGATGGCGATTATGATGGAGCTGAGTTTGAGGAGGAGGAATCCCCTGAGAAGGTTAACATTGTGTTGCAACGGGTTCTACTATGTCCTAAAGAAGAGGGGCAGCGGCGCAATATTTTCAGGTCACTTTGTTCCATTAATAACAAAGTGTGTAATTTAATTGTGGATAATGGGAGCTGCGAAAATTTTGTAGCCAAGAAACTGGTGGAGTATTTGCAGTTACCGGCGCAGCCTCATGAGGCACCTTATTCCCTCGGGTGGGTCAAGAAAAGTCCACAAGTTCGAGTTGTTGAATCCTGCAAAGTGCCGGTATCCATTGGTAAGCATTATAAAGATGAAGTTTTGTGCGACATTATTGATATGGATGCTTGTCATATTTTGTTTGGCCGACCTTGGCAATATGATGTGGATGTTACTTATAAAGACAGGGACAATGTGATGTTGTTTATGTGGAATAACCATAAAATTGCTATGGCTCCTGTGTGTCAATTTGAGAAATCTGATGTGAAGAAAGGGGAGAGTTTCCTGACCTTATCTAGCAGTGAACTTGAGATGGAGAGGGCCTTTAAAGAATCTGAGGTTTTCTGTCCGGTGGTGATAAAGGGGTTGTTGTCTGCAGAAAAGGAAGAAGTAGTGATTCCCAAAGAAGTACAGGACATGTTGGGAGATTTTGGAGAGTTGATCTCGGATGAGTTGCCCAACGAGCTGCCACCTATGAGAGACATTCAACATCAGATTGATTTGGTGCCTGGAGCTAGCTTGCCCAATCTGCCACATTACCGAATGAGTCCCAAGGAGAATGAGATTTTGAGAGAGCAGATTGAAGACTTGTTAAAAAAAGGGTTCATTCGAGAGAGTATGAGTCCTTGTGCAGTTCCAGTCCTCCTTGTTCCTAAAAAGGGCAATCAATGGCGGATGTGTGTTGATAGCAGGGCCATTAACAAGATAACTGTGAAGTACAGGTTTCCTATTCCTCGTTTGGAAGACATGCTTGATGAGCTAGAAGGTTCCAAAGTGTTTACCAAGATAGACCTTCGAAGTGGATATCACCAGATTCGAATCAAGCCAGGGGATGAATGGAAGACAGCTTTTAAGAGCAAGGATGGCTTGTACGAGTGGATGGTTATGCCATTCGGGTTGTCTAATGCACCCAGCACTTTTATGAGGCTTATGAACCAGGTTCTTCGCCCTTTTACAGGTTCCTTCGTAGTAGTGTATTATGATGATATACTGATTTATAGCAGGACCAAAGAAGAACATCTGGTGCATTTGAAGCAGGTTTTGGAAGCTTTACAGGAGAATAAACTGTACATCAACTTAAAAAAATGTACATTCTGCACCAGTAAGTTATTATTTCTGGAATTTGTGGTTGGAGAAGAAGGCATTCAGGTTGATGAAGAAAAGGTACGAGCAATAAGAGAATGGCCAGCTCCAAAAACAGTTTCTGAAGTGTGGAGTTTTCATGGTTTAGCTACTTTCTACAGAAGATTTGTGAAGAATTTCAGTACCATTACTGCCCCTATTACTGAATGTTTGAAGAAAGGCAAATTCCAATGGGGAGAGGAACAAGAGAAAAGTTTTGCTTTAATCAAGGAGAAACTTTGTACTGCACCAGTTCTTGCCCTTCCTAATTTTGACAAGATATTCGAGGTTGAATGTGATGCTAGTGGCGTGGGAGTAGGTGCTGTGTTGTCACAAGAAAAGAAACCAGTAGCATTCTTTAGTGAAAAGCTGAGTGAAGCTCGTCAGAAGTGGAGTACTTATGACCAAGAATTTTATGCAGTGTTCCGGGCATTGAGGCAATGGGAGCACTACCTGATTCAGAGGGAGTTCGTACTGTTCGCCGATCACCAAGCCTTGAAGTACCTCAATAGCCAGAAAACTGTGAATAAAATGCATGCGAGGTGGGTGAGTTTTCTGCAGAAATTTCCTTTTGTGATTCAACATAAATCTGGTTCTCTTAACCGGGTCGCAGATGCTTTGAGTAGGAGGGCGTCCTTGCTGGTCACTTTAGCTCAGGAGATTGTGGGGTTTGATCTCTTGAAGGAGTTGTATGAGGAAGATGTTGATTTCAAAGAGATTTGGGCCAAGTGCAGCAATAACAATGCAGTCGCAGATTTTTATGCGAATGGAGTGTTGTGCATAGCGCCACAGGGAAGTCACCATTCTCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 Retrotrans_gag rve zf-CCHC zf-H2C2

Protein Analysis

1103

Amino Acids

127.05

Weight (kDa)

6.9

Isoelectric Point (pI)

49.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ty3_capsid PF19259 133 - 268 6.9e-07 Ty3 transposon capsid-like protein
Retrotrans_gag PF03732 142 - 240 1e-13 Retrotransposon gag protein
RVT_1 PF00078 676 - 832 1.3e-41 Reverse transcriptase (RNA-dependent DNA polymerase)
RT_RNaseH_2 PF17919 898 - 991 1.3e-30 RNase H-like domain found in reverse transcriptase
RT_RNaseH PF17917 923 - 1022 5.5e-32 RNase H-like domain found in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000291)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG00860
fragaria_vesca FvH4_1g23712 FvH4_1g29809 FvH4_1g29810 FvH4_2g02661 FvH4_2g13451 FvH4_2g17921 FvH4_2g17922 FvH4_2g19251 FvH4_2g19252 FvH4_2g28221 FvH4_2g35961 FvH4_3g10372 FvH4_3g12461 FvH4_3g12461 FvH4_3g27861 FvH4_3g34591 FvH4_3g40201 FvH4_3g44612 FvH4_4g08352 FvH4_4g12251 FvH4_4g12742 FvH4_4g13904 FvH4_4g14287 FvH4_4g15243 FvH4_4g24071 FvH4_4g33448 FvH4_5g21912 FvH4_5g39371 FvH4_6g14992 FvH4_6g15221 FvH4_6g20603 FvH4_6g31093 FvH4_6g32372 FvH4_6g33012
malus_domestica MD11G1009100.v1.1 MD13G1145000.v1.1 MD16G1138700.v1.1
prunus_persica Prupe.5G246300_v2.0.a1 Prupe.7G034800_v2.0.a1 Prupe.8G084500_v2.0.a1
pyrus_communis pycom01g18240 pycom04g00330 pycom05g09010 pycom06g01670 pycom06g01690 pycom06g07180 pycom06g11620 pycom10g05720 pycom1134g00040 pycom1134g00060 pycom12486g00010 pycom12555g00230 pycom13g21670 pycom13g23130 pycom13g23140 pycom14g10580
rosa_chinensis RchiOBHm_Chr1g0330691 RchiOBHm_Chr2g0106171 RchiOBHm_Chr2g0134731 RchiOBHm_Chr2g0142791 RchiOBHm_Chr2g0149861 RchiOBHm_Chr3g0452401 RchiOBHm_Chr3g0461881 RchiOBHm_Chr4g0401341 RchiOBHm_Chr5g0075641 RchiOBHm_Chr6g0265961 RchiOBHm_Chr7g0200201 RchiOBHm_Chr7g0237811
rosa_laevigata RLG00000002773
rosa_multiflora Rmu_co8037806.1_g000001 Rmu_co8445989.1_g000001 Rmu_sc0000745.1_g000039 Rmu_sc0004448.1_g000029 Rmu_sc0004488.1_g000002 Rmu_sc0004591.1_g000018 Rmu_sc0005065.1_g000030 Rmu_sc0005150.1_g000005 Rmu_sc0005654.1_g000011 Rmu_sc0006986.1_g000016
rosa_roxburghii Rroxscaffold_164G00436490 Rroxscaffold_175G00432490 Rroxscaffold_1G00059440 Rroxscaffold_1G00064410 Rroxscaffold_2G00100310 Rroxscaffold_2G00128170 Rroxscaffold_3G00239420 Rroxscaffold_3G00264970 Rroxscaffold_3G00271550 Rroxscaffold_4G00300240 Rroxscaffold_5G00385050 Rroxscaffold_6G00399720
rosa_rugosa Rorug02G0252200
rosa_samantha Rh2DG251900 Rh7CG329900
rosa_wichuraiana Rw0G004800 Rw1G000850 Rw1G022350 Rw2G027030 Rw2G027760 Rw2G028410 Rw2G029130 Rw2G029390 Rw2G032490 Rw2G039460 Rw2G047800 Rw2G050340 Rw3G026430 Rw4G018370 Rw4G030750 Rw4G033140 Rw5G011950 Rw5G012010 Rw5G029280 Rw5G040190 Rw5G044570 Rw5G048240 Rw6G000340 Rw6G010140 Rw6G026480 Rw7G033750 Rw7G037250 Rw7G042080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1467, 1557
Acc36I ACCTGC 1 cut(s) 2404
AccB1I GGYRCC 2 cut(s) 1378, 1882
AccB7I CCANNNNNTGG 1 cut(s) 691
AccII CGCG 1 cut(s) 947
AciI CCGC 4 cut(s) 5, 947, 1243, 2050
AcoI YGGCCR 3 cut(s) 762, 1522, 2561
AcsI RAATTY 9 cut(s) 117, 258, 315, 1321, 2498, 2635, 2684, 2909, 3062
AcuI CTGAAG 5 cut(s) 270, 353, 699, 734, 2604
AcvI CACGTG 1 cut(s) 212
AcyI GRCGYC 1 cut(s) 3131
AdeI CACNNNGTG 1 cut(s) 215
AfiI CCNNNNNNNGG 7 cut(s) 691, 941, 1189, 1395, 1396, 2036, 2333
AflIII ACRYGT 1 cut(s) 1794
AjnI CCWGG 7 cut(s) 291, 431, 603, 1054, 1885, 2203, 2313
AjuI GAANNNNNNNTTGG 6 cut(s) 2402, 2434, 2490, 2522, 2563, 2595
Alw21I GWGCWC 1 cut(s) 2949
Alw26I GTCTC 2 cut(s) 1081, 1851
AlwNI CAGNNNCTG 2 cut(s) 242, 2582
Ama87I CYCGRG 1 cut(s) 1391
ApeKI GCWGC 9 cut(s) 71, 364, 452, 895, 1240, 1314, 1366, 1843, 3231
ApoI RAATTY 9 cut(s) 117, 258, 315, 1321, 2498, 2635, 2684, 2909, 3062
ArsI GACNNNNNNTTYG 4 cut(s) 368, 400, 2765, 2797
AseI ATTAAT 1 cut(s) 1275
Asp700I GAANNNNTTC 2 cut(s) 2520, 2636
AspLEI GCGC 4 cut(s) 947, 1248, 1366, 3286
AspS9I GGNCC 5 cut(s) 7, 1698, 2070, 2382, 3222
AsuC2I CCSGG 2 cut(s) 2927, 3104
AsuHPI GGTGA 7 cut(s) 146, 227, 1744, 2177, 2978, 3058, 3290
AsuII TTCGAA 2 cut(s) 2173, 2194
AsuNHI GCTAGC 1 cut(s) 1892
AvaI CYCGRG 1 cut(s) 1391
AvaII GGWCC 1 cut(s) 2382
BalI TGGCCA 1 cut(s) 2563
BanI GGYRCC 2 cut(s) 1378, 1882
BanII GRGCYC 2 cut(s) 208, 988
BarI GAAGNNNNNNTAC 4 cut(s) 246, 278, 2526, 2558
BauI CACGAG 1 cut(s) 813
BbrPI CACGTG 1 cut(s) 212
BbsI GAAGAC 3 cut(s) 1968, 2127, 2225
Bbv12I GWGCWC 1 cut(s) 2949
BbvI GCAGC 9 cut(s) 83, 376, 439, 882, 1252, 1301, 1378, 1830, 3243
BccI CCATC 8 cut(s) 95, 173, 535, 1136, 1148, 1684, 2234, 2251
BcgI CGANNNNNNTGC 2 cut(s) 464, 498
BciT130I CCWGG 7 cut(s) 293, 433, 605, 1056, 1887, 2205, 2315
BciVI GTATCC 1 cut(s) 1459
BcnI CCSGG 2 cut(s) 2927, 3104
BcoDI GTCTC 2 cut(s) 1081, 1851
BfaI CTAG 8 cut(s) 612, 779, 966, 1017, 1674, 1893, 2138, 2802
BfmI CTRYAG 5 cut(s) 453, 896, 1752, 2617, 3056
BfoI RGCGCY 1 cut(s) 3287
BfuAI ACCTGC 1 cut(s) 2404
BfuI GTATCC 1 cut(s) 1459
BglII AGATCT 1 cut(s) 1064
BmcAI AGTACT 2 cut(s) 448, 2896
Bme1390I CCNGG 9 cut(s) 293, 433, 605, 1056, 1887, 2205, 2315, 2927, 3104
Bme18I GGWCC 1 cut(s) 2382
BmeT110I CYCGRG 1 cut(s) 1391
BmgT120I GGNCC 5 cut(s) 7, 1698, 2070, 2382, 3222
BmiI GGNNCC 6 cut(s) 207, 1380, 1614, 1884, 2146, 2338
BmrFI CCNGG 9 cut(s) 293, 433, 605, 1056, 1887, 2205, 2315, 2927, 3104
BmsI GCATC 4 cut(s) 1034, 1492, 2788, 3103
BmtI GCTAGC 1 cut(s) 1896
BpiI GAAGAC 3 cut(s) 1968, 2127, 2225
BplI GAGNNNNNCTC 4 cut(s) 1805, 1837, 2863, 2895
BpmI CTGGAG 2 cut(s) 454, 1908
Bpu10I CCTNAGC 1 cut(s) 3153
Bpu14I TTCGAA 2 cut(s) 2173, 2194
BpuEI CTTGAG 1 cut(s) 1706
BpuMI CCSGG 2 cut(s) 2927, 3104
Bsa29I ATCGAT 1 cut(s) 1042
BsaAI YACGTR 1 cut(s) 212
BsaBI GATNNNNATC 1 cut(s) 2195
BsaHI GRCGYC 1 cut(s) 3131
BsaJI CCNNGG 7 cut(s) 604, 879, 1055, 1390, 1529, 1929, 2204
BsaWI WCCGGW 1 cut(s) 1726
Bsc4I CCNNNNNNNGG 7 cut(s) 691, 941, 1189, 1395, 1396, 2036, 2333
Bse118I RCCGGY 2 cut(s) 1360, 1444
Bse1I ACTGG 5 cut(s) 1344, 2015, 2478, 2750, 2846
Bse3DI GCAATG 1 cut(s) 2945
Bse8I GATNNNNATC 1 cut(s) 2195
BseBI CCWGG 7 cut(s) 293, 433, 605, 1056, 1887, 2205, 2315
BseCI ATCGAT 1 cut(s) 1042
BseDI CCNNGG 7 cut(s) 604, 879, 1055, 1390, 1529, 1929, 2204
BseGI GGATG 8 cut(s) 1126, 1507, 1552, 1831, 2058, 2215, 2245, 2262
BseJI GATNNNNATC 1 cut(s) 2195
BseLI CCNNNNNNNGG 7 cut(s) 691, 941, 1189, 1395, 1396, 2036, 2333
BseMI GCAATG 1 cut(s) 2945
BseMII CTCAG 8 cut(s) 234, 627, 1071, 1152, 1176, 1704, 2861, 3167
BseNI ACTGG 5 cut(s) 1344, 2015, 2478, 2750, 2846
BseRI GAGGAG 3 cut(s) 1099, 1184, 1187
BseXI GCAGC 9 cut(s) 83, 376, 439, 882, 1252, 1301, 1378, 1830, 3243
BseYI CCCAGC 1 cut(s) 2288
BsgI GTGCAG 5 cut(s) 873, 2028, 2458, 2730, 3250
Bsh1236I CGCG 1 cut(s) 947
BshNI GGYRCC 2 cut(s) 1378, 1882
BshVI ATCGAT 1 cut(s) 1042
BsiHKAI GWGCWC 1 cut(s) 2949
BsiHKCI CYCGRG 1 cut(s) 1391
BsiSI CCGG 5 cut(s) 1361, 1445, 1727, 2926, 3103
BslFI GGGAC 2 cut(s) 1580, 1911
BslI CCNNNNNNNGG 7 cut(s) 691, 941, 1189, 1395, 1396, 2036, 2333
BsmAI GTCTC 2 cut(s) 1081, 1851
BsmFI GGGAC 2 cut(s) 1580, 1911
BsmI GAATGC 2 cut(s) 2520, 2852
BsoBI CYCGRG 1 cut(s) 1391
Bsp119I TTCGAA 2 cut(s) 2173, 2194
Bsp1286I GDGCHC 3 cut(s) 208, 988, 2949
Bsp1407I TGTACA 2 cut(s) 2445, 2465
Bsp143I GATC 5 cut(s) 28, 1064, 1818, 2983, 3172
BspACI CCGC 4 cut(s) 5, 947, 1243, 2050
BspCNI CTCAG 8 cut(s) 235, 628, 1072, 1153, 1177, 1705, 2862, 3166
BspDI ATCGAT 1 cut(s) 1042
BspFNI CGCG 1 cut(s) 947
BspHI TCATGA 1 cut(s) 1372
BspLI GGNNCC 6 cut(s) 207, 1380, 1614, 1884, 2146, 2338
BspMAI CTGCAG 4 cut(s) 457, 900, 1756, 3060
BspMI ACCTGC 1 cut(s) 2404
BspOI GCTAGC 1 cut(s) 1896
BspT104I TTCGAA 2 cut(s) 2173, 2194
BspT107I GGYRCC 2 cut(s) 1378, 1882
BsrDI GCAATG 1 cut(s) 2945
BsrFI RCCGGY 2 cut(s) 1360, 1444
BsrGI TGTACA 2 cut(s) 2445, 2465
BsrI ACTGG 5 cut(s) 1344, 2015, 2478, 2750, 2846
BssAI RCCGGY 2 cut(s) 1360, 1444
BssECI CCNNGG 7 cut(s) 604, 879, 1055, 1390, 1529, 1929, 2204
BssMI GATC 5 cut(s) 28, 1064, 1818, 2983, 3172
BssNI GRCGYC 1 cut(s) 3131
BssSI CACGAG 1 cut(s) 813
BssT1I CCWWGG 3 cut(s) 879, 1529, 1929
Bst2BI CACGAG 1 cut(s) 813
Bst2UI CCWGG 7 cut(s) 293, 433, 605, 1056, 1887, 2205, 2315
Bst4CI ACNGT 8 cut(s) 24, 619, 751, 2089, 2445, 2578, 2976, 3022
Bst6I CTCTTC 2 cut(s) 437, 1227
BstACI GRCGYC 1 cut(s) 3131
BstAUI TGTACA 2 cut(s) 2445, 2465
BstBAI YACGTR 1 cut(s) 212
BstBI TTCGAA 2 cut(s) 2173, 2194
BstC8I GCNNGC 7 cut(s) 194, 697, 949, 1894, 1898, 2565, 3036
BstDEI CTNAG 9 cut(s) 243, 636, 1080, 1161, 1185, 1713, 2870, 3153, 3309
BstENI CCTNNNNNAGG 1 cut(s) 2331
BstF5I GGATG 8 cut(s) 1126, 1507, 1552, 1831, 2058, 2215, 2245, 2262
BstFNI CGCG 1 cut(s) 947
BstH2I RGCGCY 1 cut(s) 3287
BstHHI GCGC 4 cut(s) 947, 1248, 1366, 3286
BstKTI GATC 5 cut(s) 31, 1067, 1821, 2986, 3175
BstMAI GTCTC 2 cut(s) 1081, 1851
BstMBI GATC 5 cut(s) 28, 1064, 1818, 2983, 3172
BstNI CCWGG 7 cut(s) 293, 433, 605, 1056, 1887, 2205, 2315
BstNSI RCATGY 4 cut(s) 428, 1798, 2128, 3038
BstSCI CCNGG 9 cut(s) 291, 431, 603, 1054, 1885, 2203, 2313, 2925, 3102
BstSFI CTRYAG 5 cut(s) 453, 896, 1752, 2617, 3056
BstUI CGCG 1 cut(s) 947
BstV1I GCAGC 9 cut(s) 83, 376, 439, 882, 1252, 1301, 1378, 1830, 3243
BstV2I GAAGAC 3 cut(s) 1968, 2127, 2225
BstX2I RGATCY 1 cut(s) 1064
BstXI CCANNNNNNTGG 1 cut(s) 1339
BstYI RGATCY 1 cut(s) 1064
Bsu15I ATCGAT 1 cut(s) 1042
BsuI GTATCC 1 cut(s) 1459
BsuTUI ATCGAT 1 cut(s) 1042
BtsCI GGATG 8 cut(s) 1126, 1507, 1552, 1831, 2058, 2215, 2245, 2262
BtsI GCAGTG 2 cut(s) 1684, 2925
BtsIMutI CAGTG 3 cut(s) 712, 1684, 2925
BveI ACCTGC 1 cut(s) 2404
Cac8I GCNNGC 7 cut(s) 194, 697, 949, 1894, 1898, 2565, 3036
CaiI CAGNNNCTG 2 cut(s) 242, 2582
CciI TCATGA 1 cut(s) 1372
CfoI GCGC 4 cut(s) 947, 1248, 1366, 3286
Cfr10I RCCGGY 2 cut(s) 1360, 1444
Cfr13I GGNCC 5 cut(s) 7, 1698, 2070, 2382, 3222
ClaI ATCGAT 1 cut(s) 1042
CseI GACGC 1 cut(s) 3120
CsiI ACCWGGT 1 cut(s) 2313
CspCI CAANNNNNGTGG 2 cut(s) 949, 984
CviAII CATG 9 cut(s) 97, 278, 425, 516, 1373, 1795, 2125, 2600, 3035
DdeI CTNAG 9 cut(s) 243, 636, 1080, 1161, 1185, 1713, 2870, 3153, 3309
DpnI GATC 5 cut(s) 30, 1066, 1820, 2985, 3174
DpnII GATC 5 cut(s) 28, 1064, 1818, 2983, 3172
DraI TTTAAA 2 cut(s) 919, 1705
DraIII CACNNNGTG 1 cut(s) 215
EaeI YGGCCR 3 cut(s) 762, 1522, 2561
Eam1104I CTCTTC 2 cut(s) 437, 1227
EarI CTCTTC 2 cut(s) 437, 1227
EciI GGCGGA 1 cut(s) 2065
Eco130I CCWWGG 3 cut(s) 879, 1529, 1929
Eco24I GRGCYC 2 cut(s) 208, 988
Eco32I GATATC 1 cut(s) 2183
Eco47I GGWCC 1 cut(s) 2382
Eco57I CTGAAG 5 cut(s) 270, 353, 699, 734, 2604
Eco72I CACGTG 1 cut(s) 212
Eco88I CYCGRG 1 cut(s) 1391
EcoNI CCTNNNNNAGG 1 cut(s) 2331
EcoO109I RGGNCCY 1 cut(s) 1698
EcoRII CCWGG 7 cut(s) 291, 431, 603, 1054, 1885, 2203, 2313
EcoRV GATATC 1 cut(s) 2183
EcoT14I CCWWGG 3 cut(s) 879, 1529, 1929
EcoT22I ATGCAT 1 cut(s) 3036
EcoT38I GRGCYC 2 cut(s) 208, 988
ErhI CCWWGG 3 cut(s) 879, 1529, 1929
FaeI CATG 9 cut(s) 100, 281, 428, 519, 1376, 1798, 2128, 2603, 3038
FalI AAGNNNNNCTT 6 cut(s) 2112, 2144, 2228, 2260, 2704, 2736
FaqI GGGAC 2 cut(s) 1580, 1911
FatI CATG 9 cut(s) 96, 277, 424, 515, 1372, 1794, 2124, 2599, 3034
FauI CCCGC 1 cut(s) 940
FokI GGATG 8 cut(s) 1133, 1514, 1559, 1838, 2065, 2222, 2252, 2269
FriOI GRGCYC 2 cut(s) 208, 988
FspBI CTAG 8 cut(s) 612, 779, 966, 1017, 1674, 1893, 2138, 2802
GlaI GCGC 4 cut(s) 946, 1247, 1365, 3285
GsaI CCCAGC 1 cut(s) 2292
GsuI CTGGAG 2 cut(s) 454, 1908
HaeII RGCGCY 1 cut(s) 3287
HapII CCGG 5 cut(s) 1361, 1445, 1727, 2926, 3103
HgaI GACGC 1 cut(s) 3120
HhaI GCGC 4 cut(s) 947, 1248, 1366, 3286
Hin1I GRCGYC 1 cut(s) 3131
Hin1II CATG 9 cut(s) 100, 281, 428, 519, 1376, 1798, 2128, 2603, 3038
Hin6I GCGC 4 cut(s) 945, 1246, 1364, 3284
HinP1I GCGC 4 cut(s) 945, 1246, 1364, 3284
HincII GTYRAC 1 cut(s) 1195
HindII GTYRAC 1 cut(s) 1195
HindIII AAGCTT 2 cut(s) 921, 2424
HpaI GTTAAC 1 cut(s) 1195
HpaII CCGG 5 cut(s) 1361, 1445, 1727, 2926, 3103
HphI GGTGA 7 cut(s) 146, 227, 1744, 2177, 2978, 3058, 3290
Hpy166II GTNNAC 6 cut(s) 78, 376, 1195, 1412, 1682, 2157
Hpy8I GTNNAC 6 cut(s) 78, 376, 1195, 1412, 1682, 2157
HpyCH4III ACNGT 8 cut(s) 24, 619, 751, 2089, 2445, 2578, 2976, 3022
HpyCH4IV ACGT 1 cut(s) 211
HpyF3I CTNAG 9 cut(s) 243, 636, 1080, 1161, 1185, 1713, 2870, 3153, 3309
HpySE526I ACGT 1 cut(s) 211
Hsp92I GRCGYC 1 cut(s) 3131
Hsp92II CATG 9 cut(s) 100, 281, 428, 519, 1376, 1798, 2128, 2603, 3038
HspAI GCGC 4 cut(s) 945, 1246, 1364, 3284
KspAI GTTAAC 1 cut(s) 1195
Kzo9I GATC 5 cut(s) 28, 1064, 1818, 2983, 3172
LmnI GCTCC 7 cut(s) 211, 1157, 1311, 1618, 1889, 2572, 2944
Lsp1109I GCAGC 9 cut(s) 83, 376, 439, 882, 1252, 1301, 1378, 1830, 3243
LweI GCATC 4 cut(s) 1034, 1492, 2788, 3103
MabI ACCWGGT 1 cut(s) 2313
MaeI CTAG 8 cut(s) 612, 779, 966, 1017, 1674, 1893, 2138, 2802
MaeII ACGT 1 cut(s) 211
MaeIII GTNAC 9 cut(s) 55, 741, 769, 1260, 1356, 1549, 2829, 3142, 3296
MalI GATC 5 cut(s) 30, 1066, 1820, 2985, 3174
MboI GATC 5 cut(s) 28, 1064, 1818, 2983, 3172
MflI RGATCY 1 cut(s) 1064
MhlI GDGCHC 3 cut(s) 208, 988, 2949
MlsI TGGCCA 1 cut(s) 2563
MluNI TGGCCA 1 cut(s) 2563
MlyI GAGTC 2 cut(s) 1933, 2008
MmeI TCCRAC 2 cut(s) 780, 2488
Mox20I TGGCCA 1 cut(s) 2563
Mph1103I ATGCAT 1 cut(s) 3036
MroXI GAANNNNTTC 2 cut(s) 2520, 2636
MscI TGGCCA 1 cut(s) 2563
MslI CAYNNNNRTG 1 cut(s) 2297
Msp20I TGGCCA 1 cut(s) 2563
MspA1I CMGCKG 1 cut(s) 1243
MspI CCGG 5 cut(s) 1361, 1445, 1727, 2926, 3103
MspR9I CCNGG 9 cut(s) 293, 433, 605, 1056, 1887, 2205, 2315, 2927, 3104
Mva1269I GAATGC 2 cut(s) 2520, 2852
MvaI CCWGG 7 cut(s) 293, 433, 605, 1056, 1887, 2205, 2315
MvnI CGCG 1 cut(s) 947
NciI CCSGG 2 cut(s) 2927, 3104
NdeII GATC 5 cut(s) 28, 1064, 1818, 2983, 3172
NheI GCTAGC 1 cut(s) 1892
NlaIII CATG 9 cut(s) 100, 281, 428, 519, 1376, 1798, 2128, 2603, 3038
NlaIV GGNNCC 6 cut(s) 207, 1380, 1614, 1884, 2146, 2338
NmuCI GTSAC 6 cut(s) 55, 769, 1260, 2829, 3142, 3296
NsiI ATGCAT 1 cut(s) 3036
NspI RCATGY 4 cut(s) 428, 1798, 2128, 3038
NspV TTCGAA 2 cut(s) 2173, 2194
PaeI GCATGC 1 cut(s) 3038
PagI TCATGA 1 cut(s) 1372
PciI ACATGT 1 cut(s) 1794
PctI GAATGC 2 cut(s) 2520, 2852
PdmI GAANNNNTTC 2 cut(s) 2520, 2636
PflMI CCANNNNNTGG 1 cut(s) 691
PleI GAGTC 2 cut(s) 1932, 2007
PmaCI CACGTG 1 cut(s) 212
PmlI CACGTG 1 cut(s) 212
PpsI GAGTC 2 cut(s) 1932, 2007
Ppu21I YACGTR 1 cut(s) 212
PscI ACATGT 1 cut(s) 1794
PshBI ATTAAT 1 cut(s) 1275
PsiI TTATAA 2 cut(s) 1467, 1557
Psp6I CCWGG 7 cut(s) 291, 431, 603, 1054, 1885, 2203, 2313
PspCI CACGTG 1 cut(s) 212
PspFI CCCAGC 1 cut(s) 2288
PspGI CCWGG 7 cut(s) 291, 431, 603, 1054, 1885, 2203, 2313
PspN4I GGNNCC 6 cut(s) 207, 1380, 1614, 1884, 2146, 2338
PspPI GGNCC 5 cut(s) 7, 1698, 2070, 2382, 3222
PstI CTGCAG 4 cut(s) 457, 900, 1756, 3060
PstNI CAGNNNCTG 2 cut(s) 242, 2582
PsuI RGATCY 1 cut(s) 1064
RseI CAYNNNNRTG 1 cut(s) 2297
Sau3AI GATC 5 cut(s) 28, 1064, 1818, 2983, 3172
Sau96I GGNCC 5 cut(s) 7, 1698, 2070, 2382, 3222
ScaI AGTACT 2 cut(s) 448, 2896
SchI GAGTC 2 cut(s) 1933, 2008
ScrFI CCNGG 9 cut(s) 293, 433, 605, 1056, 1887, 2205, 2315, 2927, 3104
SduI GDGCHC 3 cut(s) 208, 988, 2949
SexAI ACCWGGT 1 cut(s) 2313
SfaNI GCATC 4 cut(s) 1034, 1492, 2788, 3103
SfcI CTRYAG 5 cut(s) 453, 896, 1752, 2617, 3056
SfuI TTCGAA 2 cut(s) 2173, 2194
SinI GGWCC 1 cut(s) 2382
SmiMI CAYNNNNRTG 1 cut(s) 2297
SmlI CTYRAG 1 cut(s) 1685
SmoI CTYRAG 1 cut(s) 1685
SphI GCATGC 1 cut(s) 3038
SsiI CCGC 4 cut(s) 5, 947, 1243, 2050
SspI AATATT 1 cut(s) 1252
SspMI CTAG 8 cut(s) 612, 779, 966, 1017, 1674, 1893, 2138, 2802
StyD4I CCNGG 9 cut(s) 291, 431, 603, 1054, 1885, 2203, 2313, 2925, 3102
StyI CCWWGG 3 cut(s) 879, 1529, 1929
TaaI ACNGT 8 cut(s) 24, 619, 751, 2089, 2445, 2578, 2976, 3022
TaiI ACGT 1 cut(s) 214
TaqI TCGA 7 cut(s) 484, 1042, 1420, 1987, 2173, 2194, 2784
TatI WGTACW 7 cut(s) 446, 1786, 2094, 2445, 2465, 2741, 2894
TauI GCSGC 2 cut(s) 8, 1246
TscAI CASTG 3 cut(s) 712, 1684, 2925
TseFI GTSAC 6 cut(s) 55, 769, 1260, 2829, 3142, 3296
TseI GCWGC 9 cut(s) 71, 364, 452, 895, 1240, 1314, 1366, 1843, 3231
Tsp45I GTSAC 6 cut(s) 55, 769, 1260, 2829, 3142, 3296
TspRI CASTG 3 cut(s) 712, 1684, 2925
Van91I CCANNNNNTGG 1 cut(s) 691
VpaK11BI GGWCC 1 cut(s) 2382
VspI ATTAAT 1 cut(s) 1275
XagI CCTNNNNNAGG 1 cut(s) 2331
XapI RAATTY 9 cut(s) 117, 258, 315, 1321, 2498, 2635, 2684, 2909, 3062
XceI RCATGY 4 cut(s) 428, 1798, 2128, 3038
XcmI CCANNNNNNNNNTGG 2 cut(s) 1339, 2211
XmnI GAANNNNTTC 2 cut(s) 2520, 2636
XspI CTAG 8 cut(s) 612, 779, 966, 1017, 1674, 1893, 2138, 2802
ZrmI AGTACT 2 cut(s) 448, 2896
Zsp2I ATGCAT 1 cut(s) 3036
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.