RchiOBHm_Chr3g0452401

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000291
Physical Location & Seq
Reverse (-)
3037968 .. 3043999
6032 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41954

Sequence Viewer

Length: 3282 bp
ATGAGCCAAGAACAGTCAAAGAGGGTTCATGATCAGCAGCAGAAGGCCGTGAAATTTGCGGACATTTTCCCTGCTGTTCAGGGAGAGGTGGCGGACAAAGTGGTGGCCCCAAAGGACGCAGCCACGATGCAGACGGCAGAAAGCGAAGCCGATGAAGAAGAAGTTGGGAGAGGTGGCGATTATGATGGGGCTGAGTTTGCGGAGGAGGAATCCCCTGAGAAGGTTAACATTGTGTTGCAGCGGGTTCTACTATGTCCTAAAGAAGAGGGGCAGCGGCGCAATATTTTCAGGTCACTTTGTTCCATTAATAACAAAGTGTGCAATTTAATTGTGGATAATGGAAGCTGCGAAAATTTTGTAGCCAAGAAACTGGTGGAGTATTTGCAGTTACCTACGCAGCCTCATGAGGCACCTTATTCCCTCGGGTGGGTCAAGAAAGGTCCACAAGTTCGAGTTGTTGAATCCTGCAAAGTACCGGTATCCATTGGTAAGCATTATAAAGATGAAGTTTTGTGCGACATTATTGATATGGATGCTTGTCATATTTTGTTTGGCCGACCTTGGCAATATGATGTGGATGTTACTTATAAAGGCAGGGACAATGTGATGTTGTTTATGTGGAATAACCATAAAATTGCTATGGCTCCTGTGTGTCAATTTGAGAAATCTGGTGTGAAGAAAGGGGAGAGTTTCCTGACCTTGTCTAGCAGTGAACTTGAGATGGAGAGGGCCTTTAAAGAATCTGAGGTTTTCTGTCCAGTGGTGATAAAGGGGTTGTTGTCTGCAGAAAAGGAAGAAGTGGTGATTCCCAAAGAAGTGCAGGACATGTTGGGAGGATTTAAAGAGTTGATCTTGGATGAGTTGCCTAACAAGCTGCCACCTATGAGAGACATTCAACATCAGATTGATTTGGTGCCTGGAGCTAGCTTGCCCAATCTGCCACATTACCGAATGAGTCCCAAGGAGAATGAGATTTTGAGAGAGCAGATTGAAGACTTGTTGAAAAAAGGGTTCATTCGAGAGAGTATGAGTCCTTGTGCAGTTCCAGTCCTCCTTGTCCCTAAAAAGGGCAATCAATGGCGGATGTGTGTTGATAGCAGGGCCATTAATAAGATAACTGTGAAGTACAGGCTTCCTATTCCTCATTTGGAAGATATGCTTGATGAGCTAGAAGGTTCCAAAGTGTTTACCAAGATAGACCTTCGAAGTGGATATCACCAGATTCGAATCAAGCCAGGGGATGAATGGAAGACAGCTTTTAAGAGCAAGGATGGCTTGTACGAGTGGATGGTTATGCCATTCGGGTTGTCTAATGCACCCAACACTTTTATGAGGCTTATGAACCAGGTTGATGAAGAAAAGGTACGAGCAATAAGAGAATGGCCAGCTCCAAAAACAGTTTCTGAAGTGCGGAGTTTTCATGGTTTAGCTACTTTCTACAGAAGATTTGTGAAGAATTTCAGTACCATTACTGCCCCTATTACTGAATGTTTGAAGAAAGGCAAATTCCAATGGGGAGAGGAACAAGAGAAAAGTTTTGCTCTAATCAAGGAGAAACTTTGTACTGCACCAGTTCTTGCTCTTCCTAATTTTGACAAGATATTCGAGGTTGAATGTGATGCTAGTGGCGTGGGAGTAGGTGCTGTGTTGTCACAAGAGAAGAAACCAGTAGCATTCTTTAGTGAAAAGCTGAGTGAAGCTCGTCAGAAGTGGAGTACTTATGACCAAGAATTTTATGTAGTGTTCTGGGCATTGAGGCAATGGGAGCACTACCTGATTCAGAGGGAGTTCGTACTGTTCACCGATCACCAAGCCTTGAAGTACCTCAATAGCCAGAAAACTGTGAATAAAATGCATGCAAGGTGGGAGGGCGTCCTTACTGGTCACTTTAGCTCAGGAGATTGTGGGGACCTTGCTATGGATTTTGTGTTGGGCTTACCCCGTACCCAAAAGGGAGTGGATTCAGTATTTGTGGTAGTTGACAGGTTCTCTAAGATGGTGCATTTCATCGCATGTAAGAAGACTGCTGATGCTTCTAATATAGCTAAACTGTTCTTCAGGGAAGTGGTTCGTTTGCATGGAGTTCCCAAGTCCATTACATCTGACAGAGACACCAAGTTCCTTAGCCATTTCTGGATTACCTTGTGGAGGATGTTTGGAACATCTTTGAACCGTAGTAGCACAGCTCATCCTCAAACGGATGGACAGACAGAGGTTACTAACAGAACTTTGGGTAATATGGTCCGAAGCGTTTGTGGAGATCGACCCAAACAGTGGGATTATGCTTTGCCCCGGGTGGAGTTTGCTTATAACAGTGTTGTGCATAGCGCCATAGGGAAGTCACCATTCTCCTTAGTTTATACTACTGTTCCTCAACATGTGGTTGATCTGGTGAAGCTTCCTAAGGTTCCTGGAGTTAGTGTTGTTGCTGATAGTATGGCTAGAGATGTACAAGCTGTTCGAGATGAAGTTAAGGCCAAGCTGGAAGCAACTAATGCTAAGAATAAAGCTGCAGCTGATAAACATCGCAGAGTGAAATTGTTCCAAGAGGGTGATGACGTGATGGTATTTCTGAGGAATGAGAGATTTCCTGTTGGTACCTATAACAAACTGAAGCCCAGAAAATATGGTCATTTTAAGGTGCTGCGGAAGATTAACGACAATGCTTATGTTATGAGAACTCGGGTCGGGCAAAACCTCAAACGTGGCGCTGCCGCGACTATGCAGTCCGCTGCTGCACAGAATCAAAGGGCTGGTCATGTGGGACCAAATGACATGAATATTGTCCAAGATGACTTTACCATAACGGAGACTGATCTTCCCGGCCGCCGTATTTTCACGGAGTCAGTTGTTGGTGAATATATCCAGAGAGCTCCTTTGGTAGCACCCTCCACTGTTCAAGTTAGTGGCGGTCAAATCACCATTGGTGAAGCCTTGGAGGCTACTGCTATGACGGCCGGAAAGAAGCCCGTGGACTGGAGTGACGCTGCTGCAATCCAAGAAGCTGAGATTAGAGCCACCGGGCGACCCAGCATTGTACCTGGTGGTGTGGCTGCGGAGGCTCAGTCTGCCGCAACCCTCAACGCTCGGACTATGCGCAATGAGGACAAGACCAAACTTGGTGATATTCTTGCGGGTGCAACCGCAAAACTACCAGATGACAAACCAGCAACACTGCGAGACGCCGAGGGTGTGACAGATGCAGAGATGCGAAATGATCGATATCTGAATACACATCCAACATCCAACCGGTGTGGCAGCTTCGGTGGCTGCAGCTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 Retrotrans_gag rve zf-CCHC zf-H2C2

Protein Analysis

1093

Amino Acids

122.79

Weight (kDa)

8.36

Isoelectric Point (pI)

42.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMP PF04927 17 - 57 6.6e-07 Seed maturation protein
RVT_1 PF00078 353 - 453 2.8e-21 Reverse transcriptase (RNA-dependent DNA polymerase)
RT_RNaseH_2 PF17919 505 - 598 2.2e-30 RNase H-like domain found in reverse transcriptase
RT_RNaseH PF17917 530 - 626 1.2e-30 RNase H-like domain found in reverse transcriptase
rve PF00665 640 - 730 1.6e-06 Integrase core domain
SH3_Tf2-1 PF24626 851 - 892 8.2e-09 Tf2-1-like, SH3 domain
SMP PF04927 973 - 1030 4.9e-22 Seed maturation protein
SMP PF04927 1038 - 1080 9e-09 Seed maturation protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000291)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG00860
fragaria_vesca FvH4_1g23712 FvH4_1g29809 FvH4_1g29810 FvH4_2g02661 FvH4_2g13451 FvH4_2g17921 FvH4_2g17922 FvH4_2g19251 FvH4_2g19252 FvH4_2g28221 FvH4_2g35961 FvH4_3g10372 FvH4_3g12461 FvH4_3g12461 FvH4_3g27861 FvH4_3g34591 FvH4_3g40201 FvH4_3g44612 FvH4_4g08352 FvH4_4g12251 FvH4_4g12742 FvH4_4g13904 FvH4_4g14287 FvH4_4g15243 FvH4_4g24071 FvH4_4g33448 FvH4_5g21912 FvH4_5g39371 FvH4_6g14992 FvH4_6g15221 FvH4_6g20603 FvH4_6g31093 FvH4_6g32372 FvH4_6g33012
malus_domestica MD11G1009100.v1.1 MD13G1145000.v1.1 MD16G1138700.v1.1
prunus_persica Prupe.5G246300_v2.0.a1 Prupe.7G034800_v2.0.a1 Prupe.8G084500_v2.0.a1
pyrus_communis pycom01g18240 pycom04g00330 pycom05g09010 pycom06g01670 pycom06g01690 pycom06g07180 pycom06g11620 pycom10g05720 pycom1134g00040 pycom1134g00060 pycom12486g00010 pycom12555g00230 pycom13g21670 pycom13g23130 pycom13g23140 pycom14g10580
rosa_chinensis RchiOBHm_Chr1g0330691 RchiOBHm_Chr2g0106171 RchiOBHm_Chr2g0134731 RchiOBHm_Chr2g0142791 RchiOBHm_Chr2g0149861 RchiOBHm_Chr3g0452401 RchiOBHm_Chr3g0461881 RchiOBHm_Chr4g0401341 RchiOBHm_Chr5g0075641 RchiOBHm_Chr6g0265961 RchiOBHm_Chr7g0200201 RchiOBHm_Chr7g0237811
rosa_laevigata RLG00000002773
rosa_multiflora Rmu_co8037806.1_g000001 Rmu_co8445989.1_g000001 Rmu_sc0000745.1_g000039 Rmu_sc0004448.1_g000029 Rmu_sc0004488.1_g000002 Rmu_sc0004591.1_g000018 Rmu_sc0005065.1_g000030 Rmu_sc0005150.1_g000005 Rmu_sc0005654.1_g000011 Rmu_sc0006986.1_g000016
rosa_roxburghii Rroxscaffold_164G00436490 Rroxscaffold_175G00432490 Rroxscaffold_1G00059440 Rroxscaffold_1G00064410 Rroxscaffold_2G00100310 Rroxscaffold_2G00128170 Rroxscaffold_3G00239420 Rroxscaffold_3G00264970 Rroxscaffold_3G00271550 Rroxscaffold_4G00300240 Rroxscaffold_5G00385050 Rroxscaffold_6G00399720
rosa_rugosa Rorug02G0252200
rosa_samantha Rh2DG251900 Rh7CG329900
rosa_wichuraiana Rw0G004800 Rw1G000850 Rw1G022350 Rw2G027030 Rw2G027760 Rw2G028410 Rw2G029130 Rw2G029390 Rw2G032490 Rw2G039460 Rw2G047800 Rw2G050340 Rw3G026430 Rw4G018370 Rw4G030750 Rw4G033140 Rw5G011950 Rw5G012010 Rw5G029280 Rw5G040190 Rw5G044570 Rw5G048240 Rw6G000340 Rw6G010140 Rw6G026480 Rw7G033750 Rw7G037250 Rw7G042080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 498, 588, 2310
AasI GACNNNNNNGTC 1 cut(s) 2725
Acc16I TGCGCA 1 cut(s) 3098
Acc65I GGTACC 1 cut(s) 2597
AccB1I GGYRCC 3 cut(s) 409, 913, 2597
AccB7I CCANNNNNTGG 1 cut(s) 2274
AccII CGCG 1 cut(s) 2717
AcoI YGGCCR 4 cut(s) 553, 1382, 2824, 2955
AcsI RAATTY 5 cut(s) 53, 352, 1456, 1505, 1730
AcuI CTGAAG 3 cut(s) 1425, 2041, 2633
AcyI GRCGYC 2 cut(s) 1872, 3183
AfiI CCNNNNNNNGG 9 cut(s) 220, 426, 427, 1066, 1067, 1918, 2148, 2274, 2976
AflIII ACRYGT 2 cut(s) 825, 2377
AgeI ACCGGT 2 cut(s) 475, 3249
AgsI TTSAA 9 cut(s) 461, 896, 992, 1003, 1495, 1613, 1819, 2170, 2900
AjiI CACGTC 1 cut(s) 2560
AjnI CCWGG 5 cut(s) 916, 1234, 1344, 2410, 3040
AjuI GAANNNNNNNTTGG 4 cut(s) 147, 179, 1384, 1416
Alw21I GWGCWC 2 cut(s) 1770, 2875
Alw26I GTCTC 4 cut(s) 882, 2103, 2804, 3174
AlwNI CAGNNNCTG 1 cut(s) 1403
Ama87I CYCGRG 3 cut(s) 422, 2292, 2682
AoxI GGCC 9 cut(s) 45, 105, 553, 729, 1101, 1382, 2475, 2824, 2955
ApoI RAATTY 5 cut(s) 53, 352, 1456, 1505, 1730
ArsI GACNNNNNNTTYG 4 cut(s) 1586, 1618, 2740, 2772
AseI ATTAAT 2 cut(s) 306, 1107
AsiGI ACCGGT 2 cut(s) 475, 3249
Asp700I GAANNNNTTC 3 cut(s) 1457, 2067, 2540
Asp718I GGTACC 1 cut(s) 2597
AspLEI GCGC 4 cut(s) 279, 2330, 2711, 3099
AspS9I GGNCC 7 cut(s) 106, 440, 729, 1101, 1909, 2242, 2765
AsuC2I CCSGG 4 cut(s) 2293, 2294, 2823, 3022
AsuII TTCGAA 2 cut(s) 1204, 1225
AsuNHI GCTAGC 1 cut(s) 923
AvaI CYCGRG 3 cut(s) 422, 2292, 2682
AvaII GGWCC 4 cut(s) 440, 1909, 2242, 2765
AxyI CCTNAGG 1 cut(s) 2403
BalI TGGCCA 1 cut(s) 1384
BanI GGYRCC 3 cut(s) 409, 913, 2597
BanII GRGCYC 1 cut(s) 2875
BarI GAAGNNNNNNTAC 2 cut(s) 1347, 1379
BbsI GAAGAC 3 cut(s) 999, 1256, 2027
Bbv12I GWGCWC 2 cut(s) 1770, 2875
BccI CCATC 7 cut(s) 179, 715, 1265, 1282, 1990, 2195, 2557
BceAI ACGGC 4 cut(s) 32, 150, 2814, 2970
BciT130I CCWGG 5 cut(s) 918, 1236, 1346, 2412, 3042
BciVI GTATCC 1 cut(s) 490
BclI TGATCA 1 cut(s) 31
BcnI CCSGG 4 cut(s) 2293, 2294, 2823, 3022
BcoDI GTCTC 4 cut(s) 882, 2103, 2804, 3174
BfaI CTAG 6 cut(s) 705, 924, 1169, 1623, 2442, 3280
BfmI CTRYAG 4 cut(s) 783, 1438, 2511, 3271
BfoI RGCGCY 2 cut(s) 2331, 2712
BfuI GTATCC 1 cut(s) 490
BglI GCCNNNNNGGC 1 cut(s) 2939
BmcAI AGTACT 1 cut(s) 1717
Bme1390I CCNGG 9 cut(s) 918, 1236, 1346, 2293, 2294, 2412, 2823, 3022, 3042
Bme18I GGWCC 4 cut(s) 440, 1909, 2242, 2765
BmeT110I CYCGRG 3 cut(s) 422, 2292, 2682
BmgBI CACGTC 1 cut(s) 2560
BmgT120I GGNCC 7 cut(s) 106, 440, 729, 1101, 1909, 2242, 2765
BmiI GGNNCC 9 cut(s) 108, 411, 645, 915, 1177, 1910, 2409, 2599, 2766
BmrFI CCNGG 9 cut(s) 918, 1236, 1346, 2293, 2294, 2412, 2823, 3022, 3042
BmsI GCATC 6 cut(s) 117, 523, 1609, 2020, 3190, 3198
BmtI GCTAGC 1 cut(s) 927
BpiI GAAGAC 3 cut(s) 999, 1256, 2027
BplI GAGNNNNNCTC 2 cut(s) 1684, 1716
BpmI CTGGAG 3 cut(s) 939, 2433, 2998
Bpu10I CCTNAGC 2 cut(s) 1894, 2123
Bpu14I TTCGAA 2 cut(s) 1204, 1225
BpuEI CTTGAG 1 cut(s) 737
BpuMI CCSGG 4 cut(s) 2293, 2294, 2823, 3022
Bsa29I ATCGAT 1 cut(s) 3220
BsaBI GATNNNNATC 3 cut(s) 1226, 2523, 3222
BsaHI GRCGYC 2 cut(s) 1872, 3183
BsaJI CCNNGG 9 cut(s) 421, 560, 960, 1235, 2291, 2292, 2934, 2970, 3186
BsaWI WCCGGW 2 cut(s) 475, 3249
BsaXI ACNNNNNCTCC 4 cut(s) 2406, 2436, 3050, 3080
Bsc4I CCNNNNNNNGG 9 cut(s) 220, 426, 427, 1066, 1067, 1918, 2148, 2274, 2976
Bse118I RCCGGY 2 cut(s) 475, 3249
Bse1I ACTGG 7 cut(s) 375, 758, 1046, 1571, 1667, 1885, 2981
Bse21I CCTNAGG 1 cut(s) 2403
Bse3DI GCAATG 2 cut(s) 1766, 3106
Bse8I GATNNNNATC 3 cut(s) 1226, 2523, 3222
BseBI CCWGG 5 cut(s) 918, 1236, 1346, 2412, 3042
BseCI ATCGAT 1 cut(s) 3220
BseDI CCNNGG 9 cut(s) 421, 560, 960, 1235, 2291, 2292, 2934, 2970, 3186
BseJI GATNNNNATC 3 cut(s) 1226, 2523, 3222
BseLI CCNNNNNNNGG 9 cut(s) 220, 426, 427, 1066, 1067, 1918, 2148, 2274, 2976
BseMI GCAATG 2 cut(s) 1766, 3106
BseMII CTCAG 8 cut(s) 183, 207, 735, 1682, 1908, 2564, 2997, 3077
BseNI ACTGG 7 cut(s) 375, 758, 1046, 1571, 1667, 1885, 2981
BseRI GAGGAG 1 cut(s) 218
BseX3I CGGCCG 2 cut(s) 2824, 2955
BseYI CCCAGC 1 cut(s) 3029
BsgI GTGCAG 4 cut(s) 839, 1059, 1551, 2721
Bsh1236I CGCG 1 cut(s) 2717
Bsh1285I CGRYCG 2 cut(s) 2827, 2958
BshFI GGCC 9 cut(s) 47, 107, 555, 731, 1103, 1384, 2477, 2826, 2957
BshNI GGYRCC 3 cut(s) 409, 913, 2597
BshTI ACCGGT 2 cut(s) 475, 3249
BshVI ATCGAT 1 cut(s) 3220
BsiEI CGRYCG 2 cut(s) 2827, 2958
BsiHKAI GWGCWC 2 cut(s) 1770, 2875
BsiHKCI CYCGRG 3 cut(s) 422, 2292, 2682
BsiSI CCGG 6 cut(s) 476, 2293, 2823, 2958, 3021, 3250
BslFI GGGAC 5 cut(s) 611, 942, 1043, 1922, 2778
BslI CCNNNNNNNGG 9 cut(s) 220, 426, 427, 1066, 1067, 1918, 2148, 2274, 2976
BsmAI GTCTC 4 cut(s) 882, 2103, 2804, 3174
BsmBI CGTCTC 1 cut(s) 3174
BsmFI GGGAC 5 cut(s) 611, 942, 1043, 1922, 2778
BsmI GAATGC 1 cut(s) 1673
BsnI GGCC 9 cut(s) 47, 107, 555, 731, 1103, 1384, 2477, 2826, 2957
BsoBI CYCGRG 3 cut(s) 422, 2292, 2682
Bsp119I TTCGAA 2 cut(s) 1204, 1225
Bsp1286I GDGCHC 2 cut(s) 1770, 2875
Bsp1407I TGTACA 1 cut(s) 2449
Bsp143I GATC 7 cut(s) 31, 849, 1804, 2260, 2386, 2815, 3217
BspANI GGCC 9 cut(s) 47, 107, 555, 731, 1103, 1384, 2477, 2826, 2957
BspCNI CTCAG 8 cut(s) 184, 208, 736, 1683, 1907, 2565, 2998, 3076
BspDI ATCGAT 1 cut(s) 3220
BspFNI CGCG 1 cut(s) 2717
BspHI TCATGA 2 cut(s) 28, 403
BspLI GGNNCC 9 cut(s) 108, 411, 645, 915, 1177, 1910, 2409, 2599, 2766
BspMAI CTGCAG 3 cut(s) 787, 2515, 3275
BspOI GCTAGC 1 cut(s) 927
BspQI GCTCTTC 1 cut(s) 1587
BspT104I TTCGAA 2 cut(s) 1204, 1225
BspT107I GGYRCC 3 cut(s) 409, 913, 2597
BsrDI GCAATG 2 cut(s) 1766, 3106
BsrFI RCCGGY 2 cut(s) 475, 3249
BsrGI TGTACA 1 cut(s) 2449
BsrI ACTGG 7 cut(s) 375, 758, 1046, 1571, 1667, 1885, 2981
BssAI RCCGGY 2 cut(s) 475, 3249
BssECI CCNNGG 9 cut(s) 421, 560, 960, 1235, 2291, 2292, 2934, 2970, 3186
BssMI GATC 7 cut(s) 31, 849, 1804, 2260, 2386, 2815, 3217
BssNI GRCGYC 2 cut(s) 1872, 3183
BssT1I CCWWGG 3 cut(s) 560, 960, 2934
Bst2UI CCWGG 5 cut(s) 918, 1236, 1346, 2412, 3042
Bst6I CTCTTC 2 cut(s) 258, 1587
BstACI GRCGYC 2 cut(s) 1872, 3183
BstAPI GCANNNNNTGC 1 cut(s) 2495
BstAUI TGTACA 1 cut(s) 2449
BstBI TTCGAA 2 cut(s) 1204, 1225
BstC8I GCNNGC 4 cut(s) 925, 929, 1386, 1857
BstDSI CCRYGG 1 cut(s) 2970
BstENI CCTNNNNNAGG 1 cut(s) 2146
BstFNI CGCG 1 cut(s) 2717
BstH2I RGCGCY 2 cut(s) 2331, 2712
BstHHI GCGC 4 cut(s) 279, 2330, 2711, 3099
BstKTI GATC 7 cut(s) 34, 852, 1807, 2263, 2389, 2818, 3220
BstMAI GTCTC 4 cut(s) 882, 2103, 2804, 3174
BstMBI GATC 7 cut(s) 31, 849, 1804, 2260, 2386, 2815, 3217
BstMCI CGRYCG 2 cut(s) 2827, 2958
BstNI CCWGG 5 cut(s) 918, 1236, 1346, 2412, 3042
BstNSI RCATGY 4 cut(s) 829, 1859, 2016, 2381
BstSCI CCNGG 9 cut(s) 916, 1234, 1344, 2291, 2292, 2410, 2821, 3020, 3040
BstSFI CTRYAG 4 cut(s) 783, 1438, 2511, 3271
BstUI CGCG 1 cut(s) 2717
BstV2I GAAGAC 3 cut(s) 999, 1256, 2027
BstXI CCANNNNNNTGG 1 cut(s) 370
BstZI CGGCCG 2 cut(s) 2824, 2955
Bsu15I ATCGAT 1 cut(s) 3220
Bsu36I CCTNAGG 1 cut(s) 2403
BsuI GTATCC 1 cut(s) 490
BsuRI GGCC 9 cut(s) 47, 107, 555, 731, 1103, 1384, 2477, 2826, 2957
BsuTUI ATCGAT 1 cut(s) 3220
BtgI CCRYGG 1 cut(s) 2970
BtgZI GCGATG 2 cut(s) 1993, 2510
BtrI CACGTC 1 cut(s) 2560
BtsI GCAGTG 2 cut(s) 715, 3173
BtsIMutI CAGTG 6 cut(s) 715, 765, 2279, 2320, 2892, 3173
Cac8I GCNNGC 4 cut(s) 925, 929, 1386, 1857
CaiI CAGNNNCTG 1 cut(s) 1403
CciI TCATGA 2 cut(s) 28, 403
CfoI GCGC 4 cut(s) 279, 2330, 2711, 3099
Cfr10I RCCGGY 2 cut(s) 475, 3249
Cfr13I GGNCC 7 cut(s) 106, 440, 729, 1101, 1909, 2242, 2765
Cfr9I CCCGGG 1 cut(s) 2292
ClaI ATCGAT 1 cut(s) 3220
CseI GACGC 4 cut(s) 125, 1861, 2993, 3191
CsiI ACCWGGT 2 cut(s) 1344, 3040
CspAI ACCGGT 2 cut(s) 475, 3249
CspCI CAANNNNNGTGG 2 cut(s) 3235, 3270
DpnI GATC 7 cut(s) 33, 851, 1806, 2262, 2388, 2817, 3219
DpnII GATC 7 cut(s) 31, 849, 1804, 2260, 2386, 2815, 3217
DraI TTTAAA 2 cut(s) 736, 841
DrdI GACNNNNNNGTC 1 cut(s) 2725
DseDI GACNNNNNNGTC 1 cut(s) 2725
EaeI YGGCCR 4 cut(s) 553, 1382, 2824, 2955
EagI CGGCCG 2 cut(s) 2824, 2955
Eam1104I CTCTTC 2 cut(s) 258, 1587
EarI CTCTTC 2 cut(s) 258, 1587
EciI GGCGGA 2 cut(s) 107, 1096
Ecl136II GAGCTC 1 cut(s) 2873
EclXI CGGCCG 2 cut(s) 2824, 2955
Eco130I CCWWGG 3 cut(s) 560, 960, 2934
Eco24I GRGCYC 1 cut(s) 2875
Eco32I GATATC 2 cut(s) 1214, 3224
Eco47I GGWCC 4 cut(s) 440, 1909, 2242, 2765
Eco52I CGGCCG 2 cut(s) 2824, 2955
Eco53kI GAGCTC 1 cut(s) 2873
Eco57I CTGAAG 3 cut(s) 1425, 2041, 2633
Eco81I CCTNAGG 1 cut(s) 2403
Eco88I CYCGRG 3 cut(s) 422, 2292, 2682
EcoICRI GAGCTC 1 cut(s) 2873
EcoNI CCTNNNNNAGG 1 cut(s) 2146
EcoO109I RGGNCCY 2 cut(s) 729, 1909
EcoRII CCWGG 5 cut(s) 916, 1234, 1344, 2410, 3040
EcoRV GATATC 2 cut(s) 1214, 3224
EcoT14I CCWWGG 3 cut(s) 560, 960, 2934
EcoT22I ATGCAT 1 cut(s) 1857
EcoT38I GRGCYC 1 cut(s) 2875
ErhI CCWWGG 3 cut(s) 560, 960, 2934
Esp3I CGTCTC 1 cut(s) 3174
FalI AAGNNNNNCTT 4 cut(s) 1143, 1175, 1259, 1291
FaqI GGGAC 5 cut(s) 611, 942, 1043, 1922, 2778
FauI CCCGC 2 cut(s) 234, 3127
FbaI TGATCA 1 cut(s) 31
FriOI GRGCYC 1 cut(s) 2875
FspBI CTAG 6 cut(s) 705, 924, 1169, 1623, 2442, 3280
FspI TGCGCA 1 cut(s) 3098
GlaI GCGC 4 cut(s) 278, 2329, 2710, 3098
GsaI CCCAGC 1 cut(s) 3033
GsuI CTGGAG 3 cut(s) 939, 2433, 2998
HaeII RGCGCY 2 cut(s) 2331, 2712
HaeIII GGCC 9 cut(s) 47, 107, 555, 731, 1103, 1384, 2477, 2826, 2957
HapII CCGG 6 cut(s) 476, 2293, 2823, 2958, 3021, 3250
HgaI GACGC 4 cut(s) 125, 1861, 2993, 3191
HhaI GCGC 4 cut(s) 279, 2330, 2711, 3099
Hin1I GRCGYC 2 cut(s) 1872, 3183
Hin6I GCGC 4 cut(s) 277, 2328, 2709, 3097
HinP1I GCGC 4 cut(s) 277, 2328, 2709, 3097
HincII GTYRAC 2 cut(s) 226, 1981
HindII GTYRAC 2 cut(s) 226, 1981
HindIII AAGCTT 1 cut(s) 2396
HpaI GTTAAC 1 cut(s) 226
HpaII CCGG 6 cut(s) 476, 2293, 2823, 2958, 3021, 3250
Hpy166II GTNNAC 7 cut(s) 226, 443, 713, 1188, 1800, 1981, 2974
Hpy188III TCNNGA 9 cut(s) 29, 404, 433, 694, 1019, 1896, 2134, 2462, 2866
Hpy8I GTNNAC 7 cut(s) 226, 443, 713, 1188, 1800, 1981, 2974
HpyAV CCTTC 4 cut(s) 37, 214, 1166, 1211
HpyCH4IV ACGT 2 cut(s) 2559, 2704
HpySE526I ACGT 2 cut(s) 2559, 2704
Hsp92I GRCGYC 2 cut(s) 1872, 3183
HspAI GCGC 4 cut(s) 277, 2328, 2709, 3097
KpnI GGTACC 1 cut(s) 2601
Ksp22I TGATCA 1 cut(s) 31
KspAI GTTAAC 1 cut(s) 226
Kzo9I GATC 7 cut(s) 31, 849, 1804, 2260, 2386, 2815, 3217
LguI GCTCTTC 1 cut(s) 1587
LmnI GCTCC 5 cut(s) 649, 920, 1393, 1765, 2878
LweI GCATC 6 cut(s) 117, 523, 1609, 2020, 3190, 3198
MabI ACCWGGT 2 cut(s) 1344, 3040
MaeI CTAG 6 cut(s) 705, 924, 1169, 1623, 2442, 3280
MaeII ACGT 2 cut(s) 2559, 2704
MaeIII GTNAC 9 cut(s) 291, 387, 580, 1650, 1883, 2215, 2340, 2981, 3193
MalI GATC 7 cut(s) 33, 851, 1806, 2262, 2388, 2817, 3219
MboI GATC 7 cut(s) 31, 849, 1804, 2260, 2386, 2815, 3217
MhlI GDGCHC 2 cut(s) 1770, 2875
MlsI TGGCCA 1 cut(s) 1384
MluNI TGGCCA 1 cut(s) 1384
MlyI GAGTC 3 cut(s) 964, 1039, 2852
MmeI TCCRAC 2 cut(s) 3263, 3270
Mox20I TGGCCA 1 cut(s) 1384
Mph1103I ATGCAT 1 cut(s) 1857
MroXI GAANNNNTTC 3 cut(s) 1457, 2067, 2540
MscI TGGCCA 1 cut(s) 1384
MslI CAYNNNNRTG 1 cut(s) 1328
Msp20I TGGCCA 1 cut(s) 1384
MspA1I CMGCKG 5 cut(s) 241, 274, 2516, 2732, 3276
MspI CCGG 6 cut(s) 476, 2293, 2823, 2958, 3021, 3250
MspR9I CCNGG 9 cut(s) 918, 1236, 1346, 2293, 2294, 2412, 2823, 3022, 3042
Mva1269I GAATGC 1 cut(s) 1673
MvaI CCWGG 5 cut(s) 918, 1236, 1346, 2412, 3042
MvnI CGCG 1 cut(s) 2717
NciI CCSGG 4 cut(s) 2293, 2294, 2823, 3022
NdeII GATC 7 cut(s) 31, 849, 1804, 2260, 2386, 2815, 3217
NheI GCTAGC 1 cut(s) 923
NlaIV GGNNCC 9 cut(s) 108, 411, 645, 915, 1177, 1910, 2409, 2599, 2766
NmeAIII GCCGAG 1 cut(s) 3211
NmuCI GTSAC 6 cut(s) 291, 1650, 1883, 2340, 2981, 3193
NsbI TGCGCA 1 cut(s) 3098
NsiI ATGCAT 1 cut(s) 1857
NspI RCATGY 4 cut(s) 829, 1859, 2016, 2381
NspV TTCGAA 2 cut(s) 1204, 1225
PaeI GCATGC 1 cut(s) 1859
PagI TCATGA 2 cut(s) 28, 403
PciI ACATGT 2 cut(s) 825, 2377
PciSI GCTCTTC 1 cut(s) 1587
PctI GAATGC 1 cut(s) 1673
PdmI GAANNNNTTC 3 cut(s) 1457, 2067, 2540
PfeI GAWTC 9 cut(s) 209, 461, 740, 805, 1222, 1227, 1777, 1961, 2743
PflFI GACNNNGTC 1 cut(s) 700
PflMI CCANNNNNTGG 1 cut(s) 2274
PfoI TCCNGGA 1 cut(s) 2410
PinAI ACCGGT 2 cut(s) 475, 3249
PleI GAGTC 3 cut(s) 963, 1038, 2851
PpsI GAGTC 3 cut(s) 963, 1038, 2851
PpuMI RGGWCCY 1 cut(s) 1909
PscI ACATGT 2 cut(s) 825, 2377
PshBI ATTAAT 2 cut(s) 306, 1107
PsiI TTATAA 3 cut(s) 498, 588, 2310
Psp124BI GAGCTC 1 cut(s) 2875
Psp5II RGGWCCY 1 cut(s) 1909
Psp6I CCWGG 5 cut(s) 916, 1234, 1344, 2410, 3040
PspFI CCCAGC 1 cut(s) 3029
PspGI CCWGG 5 cut(s) 916, 1234, 1344, 2410, 3040
PspN4I GGNNCC 9 cut(s) 108, 411, 645, 915, 1177, 1910, 2409, 2599, 2766
PspPI GGNCC 7 cut(s) 106, 440, 729, 1101, 1909, 2242, 2765
PspPPI RGGWCCY 1 cut(s) 1909
PsrI GAACNNNNNNTAC 2 cut(s) 2442, 2474
PstI CTGCAG 3 cut(s) 787, 2515, 3275
PstNI CAGNNNCTG 1 cut(s) 1403
PsyI GACNNNGTC 1 cut(s) 700
PvuII CAGCTG 2 cut(s) 2516, 3276
RseI CAYNNNNRTG 1 cut(s) 1328
SacI GAGCTC 1 cut(s) 2875
SapI GCTCTTC 1 cut(s) 1587
Sau3AI GATC 7 cut(s) 31, 849, 1804, 2260, 2386, 2815, 3217
Sau96I GGNCC 7 cut(s) 106, 440, 729, 1101, 1909, 2242, 2765
ScaI AGTACT 1 cut(s) 1717
SchI GAGTC 3 cut(s) 964, 1039, 2852
ScrFI CCNGG 9 cut(s) 918, 1236, 1346, 2293, 2294, 2412, 2823, 3022, 3042
SduI GDGCHC 2 cut(s) 1770, 2875
SexAI ACCWGGT 2 cut(s) 1344, 3040
SfaNI GCATC 6 cut(s) 117, 523, 1609, 2020, 3190, 3198
SfcI CTRYAG 4 cut(s) 783, 1438, 2511, 3271
SfuI TTCGAA 2 cut(s) 1204, 1225
SinI GGWCC 4 cut(s) 440, 1909, 2242, 2765
SmaI CCCGGG 1 cut(s) 2294
SmiMI CAYNNNNRTG 1 cut(s) 1328
SmlI CTYRAG 1 cut(s) 716
SmoI CTYRAG 1 cut(s) 716
SphI GCATGC 1 cut(s) 1859
SspI AATATT 2 cut(s) 283, 2782
SspMI CTAG 6 cut(s) 705, 924, 1169, 1623, 2442, 3280
SstI GAGCTC 1 cut(s) 2875
StyD4I CCNGG 9 cut(s) 916, 1234, 1344, 2291, 2292, 2410, 2821, 3020, 3040
StyI CCWWGG 3 cut(s) 560, 960, 2934
TaiI ACGT 2 cut(s) 2562, 2707
TaqI TCGA 8 cut(s) 451, 1018, 1204, 1225, 1605, 2263, 2461, 3220
TatI WGTACW 4 cut(s) 1125, 1562, 1715, 2449
TauI GCSGC 4 cut(s) 277, 2717, 2829, 3074
TfiI GAWTC 9 cut(s) 209, 461, 740, 805, 1222, 1227, 1777, 1961, 2743
TscAI CASTG 6 cut(s) 715, 765, 2279, 2320, 2899, 3180
TseFI GTSAC 6 cut(s) 291, 1650, 1883, 2340, 2981, 3193
Tsp45I GTSAC 6 cut(s) 291, 1650, 1883, 2340, 2981, 3193
TspGWI ACGGA 3 cut(s) 2213, 2822, 2855
TspMI CCCGGG 1 cut(s) 2292
TspRI CASTG 6 cut(s) 715, 765, 2279, 2320, 2899, 3180
Tth111I GACNNNGTC 1 cut(s) 700
Van91I CCANNNNNTGG 1 cut(s) 2274
VpaK11BI GGWCC 4 cut(s) 440, 1909, 2242, 2765
VspI ATTAAT 2 cut(s) 306, 1107
XagI CCTNNNNNAGG 1 cut(s) 2146
XapI RAATTY 5 cut(s) 53, 352, 1456, 1505, 1730
XceI RCATGY 4 cut(s) 829, 1859, 2016, 2381
XcmI CCANNNNNNNNNTGG 2 cut(s) 370, 1242
XmaI CCCGGG 1 cut(s) 2292
XmnI GAANNNNTTC 3 cut(s) 1457, 2067, 2540
XspI CTAG 6 cut(s) 705, 924, 1169, 1623, 2442, 3280
ZrmI AGTACT 1 cut(s) 1717
Zsp2I ATGCAT 1 cut(s) 1857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.