FvH4_4g08352

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000291
Physical Location & Seq
Forward (+)
8287865 .. 8291254
3390 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g08352.t1

Sequence Viewer

Length: 3390 bp
ATGCCTCCAAGGAGGGGACGTGGAGGAGCGACTCAGGGACGTGGCAACGATGAAGGGGTTGAGCGTTTGGCTCAACAAGTTGAGCGCCTCACCCAACAGGTGGAGGCCTTAGTTGTTCAGCAACAACATGATGAAGATGAATTTGATGACGAAATCAATCCTTTCAGTGAAGAAGGTGAGAGAGGAGAGCGGAGGCTGGTTCAGAATGTTGATACAAGGCGATGGGAGACGGGCATGAGGATTGACATTCCTGAATTCCACGGCAGTCTTCAGCCTGAGGAATTCGTGGATTGGCTAAATTCTGTGGAGGAGGTTTTGGAGTTCAAGGGGGTTCCTGAAGACAGAATAGTGTCACTTGTAGCTACCAGATTTCGTGGGCGCGCCTCAGCTTGGTGGCGACAATTGAAAGCAACTCGTTCGCGACAAGGCAAAAATAAGATCACAGTGTGGGAGAAAATGAAGAAGCACATGCGCAGCTCTTTCCTACCACCCAACTACTCTAGGTTGATCTATCAGCAGCTGCAAAATCTGAAGCAAGGAAACAGAACAGTGACGGAGTATACCACCGAATTCTATCATTTGATAGCTCGTTGCGACCTTGGGGAAACGGATGAGCAGCTGGTGTCAAGGTATATTGGAGGTCTCCGTACCGAGTTTCAAGATACATTAAATTTGCTAGATCCATTTACTGTGGCAGAAGCACAACAACGAGCCCTACAGTTGGAGAAGCAAATCGCTCGAAAAACAGGCAGAGGTGGGACCTGGTCCCCTAGCAGTCCAAGTAATCGAGGTGTTGTCCGCAACTCAGCCCCTAGTCGCACTTCCGCTTCACAAGTTCAGAATGTGAAACCGCACATGAGCAAACCTCCAGCCAAAATACAAGATGTGGGGGTTGGACGTACTGGAACTCGCTGTTTCAAATGTGGTGAGTCTGGACACCGGATGGCTGATTGCAAGAAAGAAGGAAGGGTTGGTAAGGGCTTGTTCATTGAGAATGAAGAAAATTCACTACAAGAGTATACTGATTTCGCACAGGCTCCTATTTATGATGACTATGGTGGGGATGATGATGTTTATGAGGAGTTTGTGAAGGGTGATGAAGGGCCGATGCTTGTTGTACAAAGGGCATGTTTTACCCCTCGAGAAGTAGAAGGTAGTGATGGGTGGCTTCGCAATAATATTTTTCAGTCCACTTGTACAATTGGTGGAAAGGTGTGTAGGTTGGTAATAGACCCAGGCAGCTGCGAAAATGTTATCTCTGAGGAAGCAGTTACCAAGCTATGCTTGGAGACACAACAACACCCTCATCCATATAAGCTTTCATGGCTGCAGAAGGCTAATGATGTGAAAGTTTCAAAGAGGGCTCTTGTTCCTTTCTCTATTGGTACAAGCTACAAAGATAAGGTGTGGTGTGATGTCGTGCCGATGGATGCTTGCCATATTCTTTTGGGACGACCGTGGGAGTATGATAGACATGTGACACATGATGGACGGAAGAACACATACAGCTTCATGTTCAATAATACTAAAATCACTCTCCTTCCAACTCGGGAACAAGTCCCAAAGCCAACCCATGGAAAGTCTTCGATGATTTTATTATCAATGAAGAAATTCGTGGGAGAAATTCATGAAACCGGGGTGGTCTACTTGTTGGTTGGTAAAGAGAAATCCAGTGATTTGTCTCCTGTCCCTGTCCCTGTCCCTGTACAAGCTTTACTAGAGGAGTTTCAAGATGTCTTTCCTGAAGAAATGCCGGCAGGACTGCCACCTTCTCGTAACATCCAACACAGAATTGATTTGGTCCCTGGAGCAGCCTTACCAAATAAGGCGCATTATCGGATGAGCCCCAAAGAACATGAAGAACTGAGACGACAAGTAGAGGAGTTATTGGCAAGAGGATACATCAGGGAAAGCATGAGTCCTTGTTCAGTTCCGGCCTTAATTACGCCAAAAAAAGATGGGACATGGCGCATGTGTATAGACAGCAGAGCCATTAATAAAATCACGGTACGTTACCGTTTTCCCATTCCTCGTTTGGATGACTTGCTAGATCAATTGTCAGGTGCTACTGTTTTCAACAAGTTAGACCTTAAAAGTGGGTATCACCAAATTCGGATCAAAGATGGGGACGAGTGGAAAACAGCCTTTAAAACTCGTGAAGGCTTGTACGAATGGTTGGTCATGCCTTTTGGCCTCTCTAATGCTCCTAGTACTTTTATGCGGGCGATGAATGAGTTGCTTCGACGGTTTATTGGAAAGTGTGTGGTGGTTTACTTTGATGACATCCTTATTTACAGCCCTAGCATTGAATTGCACCTACAACATCTAAGAGAAGTCCTAGAAGTCTTGAGAAAGGAGAAGTTTTATGCAGCAGTGAAGAAGTGTTCTTTTATGACTGACAAGGTGTTGTTCCTTGGCTATGTGGTGTCAAAAGATGGGATTCTTGTAGACCAGTCCAAAGTGGAAGCAATCAAGAGTTGGCCGATGCCGAGAAGTCTACAAGAAGTCAGAAGTTTTCATGGGTTAGTATCTTTCTATAGACGCTTTATACAAAACTTCAGTTCTATTATGGCGCCTATAACCGATTGTATGAAGAGTGGGAAATTCAGTTGGACTGAGGAGGCCACTGTAGCTTTTCAAGCCATCAAGGAGAAGCTTGTTACTGCCCCTGTTTTGACACTTCCGGGCTTTAACAAACCATTTGAATTGCATTGTGATGCATCCAAGGTCGGCATTGGGGCTGTTTTAAGCCAAGAAGGGCGACCAGTAGCTTTTTACAGTGAAAAGCTAAATGGCTCCAAGGTGAACTATAGCACATATGATGTGGAGTTTTATGCTGTTGTTCAAGCCCTCAAGTATTGGCGTTCTTATTTAGCTCATAATGAGTTTATCTTATATTCTGATCATGAGGCCTTAAAACATCTCCATGCACAAGATAAGCTCTCTGCTAGGCATGCTAAATGGGCATCATATTTGCAGCAGTTTACATTTGTGATTAAGCACAAATCTGGTGCCTTGAATAAAGTGGCGGATGCTCTTAGTAGGCGAGCGAATTTTCTCACCACCATGAATACTCAGGTGCTTGGGTTTGATTCATTGCGTGACTTATTATCTACAGATTCCTATTTTGGACCTATATTGAAGGATGTGGAATCAGGCCTACGATCTGACTTTATCCTTCATGATGGCTTTTTATTCAAAGAAAATCGATTGTGCATTCCAATGTCCAGTTTGCGTTTGAAGATCATTAGTGAGTTGCACAATGAAGGTCATATGGGGAGAGATAAGACATTGCAGCTGGTGATGAATTCATATCATTGGCCAACCCTACGACGTGAAGTCACTAAATTGTTGAAGGTTGTAGGGTGTGTCAATTATCCAAAGGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 Retrotrans_gag rve zf-CCHC zf-H2C2

Protein Analysis

1130

Amino Acids

128.95

Weight (kDa)

8.76

Isoelectric Point (pI)

48.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ty3_capsid PF19259 83 - 243 2.2e-09 Ty3 transposon capsid-like protein
Retrotrans_gag PF03732 119 - 216 1e-21 Retrotransposon gag protein
RVT_1 PF00078 650 - 808 2.5e-41 Reverse transcriptase (RNA-dependent DNA polymerase)
RT_RNaseH_2 PF17919 871 - 964 5.4e-32 RNase H-like domain found in reverse transcriptase
RT_RNaseH PF17917 897 - 995 2.9e-32 RNase H-like domain found in reverse transcriptase
Integrase_H2C2 PF17921 1076 - 1118 4.3e-10 Integrase zinc binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000291)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG00860
fragaria_vesca FvH4_1g23712 FvH4_1g29809 FvH4_1g29810 FvH4_2g02661 FvH4_2g13451 FvH4_2g17921 FvH4_2g17922 FvH4_2g19251 FvH4_2g19252 FvH4_2g28221 FvH4_2g35961 FvH4_3g10372 FvH4_3g12461 FvH4_3g12461 FvH4_3g27861 FvH4_3g34591 FvH4_3g40201 FvH4_3g44612 FvH4_4g08352 FvH4_4g12251 FvH4_4g12742 FvH4_4g13904 FvH4_4g14287 FvH4_4g15243 FvH4_4g24071 FvH4_4g33448 FvH4_5g21912 FvH4_5g39371 FvH4_6g14992 FvH4_6g15221 FvH4_6g20603 FvH4_6g31093 FvH4_6g32372 FvH4_6g33012
malus_domestica MD11G1009100.v1.1 MD13G1145000.v1.1 MD16G1138700.v1.1
prunus_persica Prupe.5G246300_v2.0.a1 Prupe.7G034800_v2.0.a1 Prupe.8G084500_v2.0.a1
pyrus_communis pycom01g18240 pycom04g00330 pycom05g09010 pycom06g01670 pycom06g01690 pycom06g07180 pycom06g11620 pycom10g05720 pycom1134g00040 pycom1134g00060 pycom12486g00010 pycom12555g00230 pycom13g21670 pycom13g23130 pycom13g23140 pycom14g10580
rosa_chinensis RchiOBHm_Chr1g0330691 RchiOBHm_Chr2g0106171 RchiOBHm_Chr2g0134731 RchiOBHm_Chr2g0142791 RchiOBHm_Chr2g0149861 RchiOBHm_Chr3g0452401 RchiOBHm_Chr3g0461881 RchiOBHm_Chr4g0401341 RchiOBHm_Chr5g0075641 RchiOBHm_Chr6g0265961 RchiOBHm_Chr7g0200201 RchiOBHm_Chr7g0237811
rosa_laevigata RLG00000002773
rosa_multiflora Rmu_co8037806.1_g000001 Rmu_co8445989.1_g000001 Rmu_sc0000745.1_g000039 Rmu_sc0004448.1_g000029 Rmu_sc0004488.1_g000002 Rmu_sc0004591.1_g000018 Rmu_sc0005065.1_g000030 Rmu_sc0005150.1_g000005 Rmu_sc0005654.1_g000011 Rmu_sc0006986.1_g000016
rosa_roxburghii Rroxscaffold_164G00436490 Rroxscaffold_175G00432490 Rroxscaffold_1G00059440 Rroxscaffold_1G00064410 Rroxscaffold_2G00100310 Rroxscaffold_2G00128170 Rroxscaffold_3G00239420 Rroxscaffold_3G00264970 Rroxscaffold_3G00271550 Rroxscaffold_4G00300240 Rroxscaffold_5G00385050 Rroxscaffold_6G00399720
rosa_rugosa Rorug02G0252200
rosa_samantha Rh2DG251900 Rh7CG329900
rosa_wichuraiana Rw0G004800 Rw1G000850 Rw1G022350 Rw2G027030 Rw2G027760 Rw2G028410 Rw2G029130 Rw2G029390 Rw2G032490 Rw2G039460 Rw2G047800 Rw2G050340 Rw3G026430 Rw4G018370 Rw4G030750 Rw4G033140 Rw5G011950 Rw5G012010 Rw5G029280 Rw5G040190 Rw5G044570 Rw5G048240 Rw6G000340 Rw6G010140 Rw6G026480 Rw7G033750 Rw7G037250 Rw7G042080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 473
AccB1I GGYRCC 2 cut(s) 2572, 3011
AccB7I CCANNNNNTGG 2 cut(s) 100, 1574
AccBSI CCGCTC 1 cut(s) 190
AccI GTMKAC 5 cut(s) 560, 1019, 1644, 2448, 2497
AccII CGCG 2 cut(s) 381, 421
AciI CCGC 6 cut(s) 190, 799, 825, 851, 2221, 3029
AclWI GGATC 2 cut(s) 674, 2123
AcoI YGGCCR 2 cut(s) 2480, 3320
AcuI CTGAAG 5 cut(s) 254, 357, 551, 1764, 2542
AcyI GRCGYC 1 cut(s) 2573
AdeI CACNNNGTG 1 cut(s) 447
AfaI GTAC 9 cut(s) 649, 901, 1119, 1198, 1387, 1707, 2010, 2168, 2212
AfiI CCNNNNNNNGG 5 cut(s) 14, 100, 390, 721, 1574
AflIII ACRYGT 1 cut(s) 1474
AhdI GACNNNNNGTC 1 cut(s) 3338
AjiI CACGTC 3 cut(s) 20, 41, 3335
AjnI CCWGG 3 cut(s) 761, 1234, 1804
AjuI GAANNNNNNNTTGG 2 cut(s) 954, 986
Alw26I GTCTC 5 cut(s) 221, 647, 1283, 1686, 1861
AlwI GGATC 2 cut(s) 674, 2123
AlwNI CAGNNNCTG 1 cut(s) 520
Ama87I CYCGRG 2 cut(s) 1140, 1548
AoxI GGCC 9 cut(s) 105, 1103, 1935, 2191, 2480, 2622, 2910, 3157, 3320
ArsI GACNNNNNNTTYG 2 cut(s) 2163, 2195
AscI GGCGCGCC 1 cut(s) 379
AseI ATTAAT 1 cut(s) 1995
Asp700I GAANNNNTTC 2 cut(s) 1582, 1610
AspLEI GCGC 7 cut(s) 87, 381, 383, 474, 1831, 1971, 2575
AspS9I GGNCC 5 cut(s) 759, 765, 1103, 1801, 3131
AsuC2I CCSGG 2 cut(s) 1636, 2685
AsuHPI GGTGA 8 cut(s) 82, 188, 938, 1106, 2096, 2815, 3052, 3313
AvaI CYCGRG 2 cut(s) 1140, 1548
AvaII GGWCC 4 cut(s) 759, 765, 1801, 3131
AxyI CCTNAGG 1 cut(s) 276
BalI TGGCCA 1 cut(s) 3322
BanI GGYRCC 2 cut(s) 2572, 3011
BanII GRGCYC 3 cut(s) 715, 1366, 1847
BarI GAAGNNNNNNTAC 2 cut(s) 1487, 1519
BauI CACGAG 1 cut(s) 2154
BbsI GAAGAC 3 cut(s) 260, 345, 1575
BbvCI CCTCAGC 1 cut(s) 385
BceAI ACGGC 1 cut(s) 277
BcgI CGANNNNNNTGC 4 cut(s) 399, 433, 719, 753
BciT130I CCWGG 3 cut(s) 763, 1236, 1806
BciVI GTATCC 1 cut(s) 1892
BclI TGATCA 1 cut(s) 2902
BcnI CCSGG 2 cut(s) 1636, 2685
BcoDI GTCTC 5 cut(s) 221, 647, 1283, 1686, 1861
BfmI CTRYAG 6 cut(s) 716, 1328, 2536, 2628, 2809, 3114
BfoI RGCGCY 2 cut(s) 88, 2576
BfuI GTATCC 1 cut(s) 1892
BmcAI AGTACT 1 cut(s) 2212
Bme1390I CCNGG 5 cut(s) 763, 1236, 1636, 1806, 2685
Bme18I GGWCC 4 cut(s) 759, 765, 1801, 3131
BmeRI GACNNNNNGTC 1 cut(s) 3338
BmeT110I CYCGRG 2 cut(s) 1140, 1548
BmgBI CACGTC 3 cut(s) 20, 41, 3335
BmgT120I GGNCC 5 cut(s) 759, 765, 1103, 1801, 3131
BmiI GGNNCC 8 cut(s) 333, 760, 767, 1038, 1803, 2574, 2797, 3013
BmrFI CCNGG 5 cut(s) 763, 1236, 1636, 1806, 2685
BmsI GCATC 7 cut(s) 1098, 1420, 2475, 2707, 2729, 2975, 3022
BpiI GAAGAC 3 cut(s) 260, 345, 1575
BplI GAGNNNNNCTC 2 cut(s) 850, 882
BpmI CTGGAG 2 cut(s) 852, 1827
Bpu10I CCTNAGC 1 cut(s) 385
BpuEI CTTGAG 2 cut(s) 2368, 2837
BpuMI CCSGG 2 cut(s) 1636, 2685
Bsa29I ATCGAT 1 cut(s) 3208
BsaHI GRCGYC 1 cut(s) 2573
BsaI GGTCTC 1 cut(s) 647
BsaWI WCCGGW 1 cut(s) 939
BsaXI ACNNNNNCTCC 1 cut(s) 34
Bsc4I CCNNNNNNNGG 5 cut(s) 14, 100, 390, 721, 1574
Bse118I RCCGGY 1 cut(s) 1753
Bse1I ACTGG 5 cut(s) 907, 1671, 2452, 2765, 3228
Bse21I CCTNAGG 1 cut(s) 276
Bse3DI GCAATG 2 cut(s) 3095, 3290
BseBI CCWGG 3 cut(s) 763, 1236, 1806
BseCI ATCGAT 1 cut(s) 3208
BseLI CCNNNNNNNGG 5 cut(s) 14, 100, 390, 721, 1574
BseMI GCAATG 2 cut(s) 3095, 3290
BseMII CTCAG 8 cut(s) 47, 267, 399, 819, 1251, 1856, 2607, 3089
BseNI ACTGG 5 cut(s) 907, 1671, 2452, 2765, 3228
BsePI GCGCGC 1 cut(s) 379
BseRI GAGGAG 7 cut(s) 39, 198, 323, 1094, 1736, 1895, 2633
Bsh1236I CGCG 2 cut(s) 381, 421
Bsh1285I CGRYCG 1 cut(s) 1457
BshFI GGCC 9 cut(s) 107, 1105, 1937, 2193, 2482, 2624, 2912, 3159, 3322
BshNI GGYRCC 2 cut(s) 2572, 3011
BshVI ATCGAT 1 cut(s) 3208
BsiEI CGRYCG 1 cut(s) 1457
BsiHKCI CYCGRG 2 cut(s) 1140, 1548
BsiSI CCGG 5 cut(s) 940, 1635, 1754, 1934, 2684
BslI CCNNNNNNNGG 5 cut(s) 14, 100, 390, 721, 1574
BsmAI GTCTC 5 cut(s) 221, 647, 1283, 1686, 1861
BsmBI CGTCTC 2 cut(s) 221, 1861
BsmI GAATGC 1 cut(s) 3216
BsnI GGCC 9 cut(s) 107, 1105, 1937, 2193, 2482, 2624, 2912, 3159, 3322
Bso31I GGTCTC 1 cut(s) 647
BsoBI CYCGRG 2 cut(s) 1140, 1548
Bsp1286I GDGCHC 3 cut(s) 715, 1366, 1847
Bsp1407I TGTACA 3 cut(s) 1117, 1196, 1705
Bsp143I GATC 8 cut(s) 438, 507, 679, 2050, 2115, 2902, 3164, 3243
Bsp19I CCATGG 1 cut(s) 1573
Bsp68I TCGCGA 1 cut(s) 421
BspACI CCGC 6 cut(s) 190, 799, 825, 851, 2221, 3029
BspANI GGCC 9 cut(s) 107, 1105, 1937, 2193, 2482, 2624, 2912, 3159, 3322
BspCNI CTCAG 8 cut(s) 46, 268, 398, 818, 1252, 1857, 2608, 3088
BspDI ATCGAT 1 cut(s) 3208
BspFNI CGCG 2 cut(s) 381, 421
BspHI TCATGA 3 cut(s) 1627, 2905, 3181
BspLI GGNNCC 8 cut(s) 333, 760, 767, 1038, 1803, 2574, 2797, 3013
BspMAI CTGCAG 1 cut(s) 1332
BspPI GGATC 2 cut(s) 674, 2123
BspT107I GGYRCC 2 cut(s) 2572, 3011
BspTNI GGTCTC 1 cut(s) 647
BsrBI CCGCTC 1 cut(s) 190
BsrDI GCAATG 2 cut(s) 3095, 3290
BsrFI RCCGGY 1 cut(s) 1753
BsrGI TGTACA 3 cut(s) 1117, 1196, 1705
BsrI ACTGG 5 cut(s) 907, 1671, 2452, 2765, 3228
BssAI RCCGGY 1 cut(s) 1753
BssHII GCGCGC 1 cut(s) 379
BssMI GATC 8 cut(s) 438, 507, 679, 2050, 2115, 2902, 3164, 3243
BssNAI GTATAC 2 cut(s) 561, 1020
BssNI GRCGYC 1 cut(s) 2573
BssSI CACGAG 1 cut(s) 2154
BssT1I CCWWGG 6 cut(s) 8, 598, 1573, 2413, 2724, 2799
Bst1107I GTATAC 2 cut(s) 561, 1020
Bst2BI CACGAG 1 cut(s) 2154
Bst2UI CCWGG 3 cut(s) 763, 1236, 1806
Bst6I CTCTTC 1 cut(s) 2588
BstACI GRCGYC 1 cut(s) 2573
BstAUI TGTACA 3 cut(s) 1117, 1196, 1705
BstC8I GCNNGC 6 cut(s) 381, 1435, 1755, 2223, 2955, 3048
BstDSI CCRYGG 3 cut(s) 259, 1457, 1573
BstFNI CGCG 2 cut(s) 381, 421
BstH2I RGCGCY 2 cut(s) 88, 2576
BstHHI GCGC 7 cut(s) 87, 381, 383, 474, 1831, 1971, 2575
BstKTI GATC 8 cut(s) 441, 510, 682, 2053, 2118, 2905, 3167, 3246
BstMAI GTCTC 5 cut(s) 221, 647, 1283, 1686, 1861
BstMBI GATC 8 cut(s) 438, 507, 679, 2050, 2115, 2902, 3164, 3243
BstMCI CGRYCG 1 cut(s) 1457
BstMWI GCNNNNNNNGC 5 cut(s) 1324, 2630, 2639, 2954, 2963
BstNI CCWGG 3 cut(s) 763, 1236, 1806
BstNSI RCATGY 5 cut(s) 472, 1131, 1478, 1975, 2957
BstSCI CCNGG 5 cut(s) 761, 1234, 1634, 1804, 2683
BstSFI CTRYAG 6 cut(s) 716, 1328, 2536, 2628, 2809, 3114
BstUI CGCG 2 cut(s) 381, 421
BstV2I GAAGAC 3 cut(s) 260, 345, 1575
BstX2I RGATCY 1 cut(s) 679
BstYI RGATCY 1 cut(s) 679
BstZ17I GTATAC 2 cut(s) 561, 1020
Bsu15I ATCGAT 1 cut(s) 3208
Bsu36I CCTNAGG 1 cut(s) 276
BsuI GTATCC 1 cut(s) 1892
BsuRI GGCC 9 cut(s) 107, 1105, 1937, 2193, 2482, 2624, 2912, 3159, 3322
BsuTUI ATCGAT 1 cut(s) 3208
BtgI CCRYGG 3 cut(s) 259, 1457, 1573
BtgZI GCGATG 2 cut(s) 235, 2240
BtrI CACGTC 3 cut(s) 20, 41, 3335
BtsI GCAGTG 1 cut(s) 2379
BtsIMutI CAGTG 7 cut(s) 172, 450, 555, 1678, 2379, 2625, 2785
BtuMI TCGCGA 1 cut(s) 421
Cac8I GCNNGC 6 cut(s) 381, 1435, 1755, 2223, 2955, 3048
CaiI CAGNNNCTG 1 cut(s) 520
CciI TCATGA 3 cut(s) 1627, 2905, 3181
CfoI GCGC 7 cut(s) 87, 381, 383, 474, 1831, 1971, 2575
Cfr10I RCCGGY 1 cut(s) 1753
Cfr13I GGNCC 5 cut(s) 759, 765, 1103, 1801, 3131
ClaI ATCGAT 1 cut(s) 3208
CseI GACGC 1 cut(s) 2550
CsiI ACCWGGT 1 cut(s) 761
Csp6I GTAC 9 cut(s) 648, 900, 1118, 1197, 1386, 1706, 2009, 2167, 2211
CviQI GTAC 9 cut(s) 648, 900, 1118, 1197, 1386, 1706, 2009, 2167, 2211
DinI GGCGCC 1 cut(s) 2574
DpnI GATC 8 cut(s) 440, 509, 681, 2052, 2117, 2904, 3166, 3245
DpnII GATC 8 cut(s) 438, 507, 679, 2050, 2115, 2902, 3164, 3243
DraI TTTAAA 1 cut(s) 2149
DraIII CACNNNGTG 1 cut(s) 447
DriI GACNNNNNGTC 1 cut(s) 3338
EaeI YGGCCR 2 cut(s) 2480, 3320
Eam1104I CTCTTC 1 cut(s) 2588
Eam1105I GACNNNNNGTC 1 cut(s) 3338
EarI CTCTTC 1 cut(s) 2588
EciI GGCGGA 1 cut(s) 3044
Eco130I CCWWGG 6 cut(s) 8, 598, 1573, 2413, 2724, 2799
Eco147I AGGCCT 3 cut(s) 107, 2912, 3159
Eco24I GRGCYC 3 cut(s) 715, 1366, 1847
Eco31I GGTCTC 1 cut(s) 647
Eco47I GGWCC 4 cut(s) 759, 765, 1801, 3131
Eco57I CTGAAG 5 cut(s) 254, 357, 551, 1764, 2542
Eco81I CCTNAGG 1 cut(s) 276
Eco88I CYCGRG 2 cut(s) 1140, 1548
EcoO109I RGGNCCY 1 cut(s) 759
EcoRI GAATTC 4 cut(s) 254, 281, 569, 3307
EcoRII CCWGG 3 cut(s) 761, 1234, 1804
EcoT14I CCWWGG 6 cut(s) 8, 598, 1573, 2413, 2724, 2799
EcoT22I ATGCAT 1 cut(s) 2722
EcoT38I GRGCYC 3 cut(s) 715, 1366, 1847
EgeI GGCGCC 1 cut(s) 2574
EheI GGCGCC 1 cut(s) 2574
ErhI CCWWGG 6 cut(s) 8, 598, 1573, 2413, 2724, 2799
Esp3I CGTCTC 2 cut(s) 221, 1861
FalI AAGNNNNNCTT 2 cut(s) 1268, 1300
FauI CCCGC 1 cut(s) 2214
FauNDI CATATG 2 cut(s) 2818, 3273
FbaI TGATCA 1 cut(s) 2902
FblI GTMKAC 5 cut(s) 560, 1019, 1644, 2448, 2497
FriOI GRGCYC 3 cut(s) 715, 1366, 1847
FspI TGCGCA 1 cut(s) 473
GlaI GCGC 7 cut(s) 86, 380, 382, 473, 1830, 1970, 2574
GsuI CTGGAG 2 cut(s) 852, 1827
HaeII RGCGCY 2 cut(s) 88, 2576
HaeIII GGCC 9 cut(s) 107, 1105, 1937, 2193, 2482, 2624, 2912, 3159, 3322
HapII CCGG 5 cut(s) 940, 1635, 1754, 1934, 2684
HgaI GACGC 1 cut(s) 2550
HhaI GCGC 7 cut(s) 87, 381, 383, 474, 1831, 1971, 2575
Hin1I GRCGYC 1 cut(s) 2573
Hin6I GCGC 7 cut(s) 85, 379, 381, 472, 1829, 1969, 2573
HinP1I GCGC 7 cut(s) 85, 379, 381, 472, 1829, 1969, 2573
HindIII AAGCTT 3 cut(s) 1316, 1710, 2654
HinfI GANTC 7 cut(s) 31, 929, 1918, 2440, 3092, 3119, 3152
HpaII CCGG 5 cut(s) 940, 1635, 1754, 1934, 2684
HphI GGTGA 8 cut(s) 82, 188, 938, 1106, 2096, 2815, 3052, 3313
Hpy166II GTNNAC 9 cut(s) 561, 1020, 1191, 1645, 2272, 2449, 2498, 2806, 2985
Hpy188I TCNGA 9 cut(s) 204, 531, 840, 1261, 1839, 2115, 2510, 2902, 3169
Hpy8I GTNNAC 9 cut(s) 561, 1020, 1191, 1645, 2272, 2449, 2498, 2806, 2985
Hpy99I CGWCG 2 cut(s) 2247, 3336
HpyCH4IV ACGT 5 cut(s) 19, 40, 898, 2011, 3334
HpyF10VI GCNNNNNNNGC 5 cut(s) 1324, 2630, 2639, 2954, 2963
HpySE526I ACGT 5 cut(s) 19, 40, 898, 2011, 3334
Hsp92I GRCGYC 1 cut(s) 2573
HspAI GCGC 7 cut(s) 85, 379, 381, 472, 1829, 1969, 2573
KasI GGCGCC 1 cut(s) 2572
KroI GCCGGC 1 cut(s) 1753
KroNI GCCGGC 1 cut(s) 1755
Ksp22I TGATCA 1 cut(s) 2902
Kzo9I GATC 8 cut(s) 438, 507, 679, 2050, 2115, 2902, 3164, 3243
LmnI GCTCC 5 cut(s) 26, 1042, 1808, 2209, 2801
LweI GCATC 7 cut(s) 1098, 1420, 2475, 2707, 2729, 2975, 3022
MabI ACCWGGT 1 cut(s) 761
MaeII ACGT 5 cut(s) 19, 40, 898, 2011, 3334
MaeIII GTNAC 9 cut(s) 351, 550, 1270, 1477, 1775, 2012, 2659, 3101, 3340
MalI GATC 8 cut(s) 440, 509, 681, 2052, 2117, 2904, 3166, 3245
MbiI CCGCTC 1 cut(s) 190
MboI GATC 8 cut(s) 438, 507, 679, 2050, 2115, 2902, 3164, 3243
MfeI CAATTG 3 cut(s) 401, 1200, 2054
MflI RGATCY 1 cut(s) 679
MhlI GDGCHC 3 cut(s) 715, 1366, 1847
MlsI TGGCCA 1 cut(s) 3322
MluNI TGGCCA 1 cut(s) 3322
Mly113I GGCGCC 1 cut(s) 2573
MlyI GAGTC 3 cut(s) 25, 938, 1927
MmeI TCCRAC 5 cut(s) 702, 874, 1568, 1807, 2591
Mox20I TGGCCA 1 cut(s) 3322
Mph1103I ATGCAT 1 cut(s) 2722
MroNI GCCGGC 1 cut(s) 1753
MroXI GAANNNNTTC 2 cut(s) 1582, 1610
MscI TGGCCA 1 cut(s) 3322
MseI TTAA 9 cut(s) 668, 1940, 1995, 2091, 2148, 2691, 2747, 2915, 2997
MslI CAYNNNNRTG 4 cut(s) 2715, 2925, 3065, 3221
Msp20I TGGCCA 1 cut(s) 3322
MspA1I CMGCKG 4 cut(s) 520, 619, 1242, 3298
MspI CCGG 5 cut(s) 940, 1635, 1754, 1934, 2684
MspR9I CCNGG 5 cut(s) 763, 1236, 1636, 1806, 2685
MunI CAATTG 3 cut(s) 401, 1200, 2054
Mva1269I GAATGC 1 cut(s) 3216
MvaI CCWGG 3 cut(s) 763, 1236, 1806
MvnI CGCG 2 cut(s) 381, 421
MwoI GCNNNNNNNGC 5 cut(s) 1324, 2630, 2639, 2954, 2963
NaeI GCCGGC 1 cut(s) 1755
NarI GGCGCC 1 cut(s) 2573
NciI CCSGG 2 cut(s) 1636, 2685
NcoI CCATGG 1 cut(s) 1573
NdeI CATATG 2 cut(s) 2818, 3273
NdeII GATC 8 cut(s) 438, 507, 679, 2050, 2115, 2902, 3164, 3243
NgoMIV GCCGGC 1 cut(s) 1753
NlaIV GGNNCC 8 cut(s) 333, 760, 767, 1038, 1803, 2574, 2797, 3013
NmeAIII GCCGAG 1 cut(s) 2514
NmuCI GTSAC 5 cut(s) 351, 550, 1477, 3101, 3340
NruI TCGCGA 1 cut(s) 421
NsbI TGCGCA 1 cut(s) 473
NsiI ATGCAT 1 cut(s) 2722
NspI RCATGY 5 cut(s) 472, 1131, 1478, 1975, 2957
PaeI GCATGC 1 cut(s) 2957
PaeR7I CTCGAG 1 cut(s) 1140
PagI TCATGA 3 cut(s) 1627, 2905, 3181
PalAI GGCGCGCC 1 cut(s) 379
PauI GCGCGC 1 cut(s) 379
PceI AGGCCT 3 cut(s) 107, 2912, 3159
PciI ACATGT 1 cut(s) 1474
PctI GAATGC 1 cut(s) 3216
PdiI GCCGGC 1 cut(s) 1755
PdmI GAANNNNTTC 2 cut(s) 1582, 1610
PfeI GAWTC 4 cut(s) 2440, 3092, 3119, 3152
PflFI GACNNNGTC 1 cut(s) 763
PflMI CCANNNNNTGG 2 cut(s) 100, 1574
PleI GAGTC 3 cut(s) 25, 937, 1926
PluTI GGCGCC 1 cut(s) 2576
PpsI GAGTC 3 cut(s) 25, 937, 1926
PpuMI RGGWCCY 1 cut(s) 759
PscI ACATGT 1 cut(s) 1474
PshBI ATTAAT 1 cut(s) 1995
Psp5II RGGWCCY 1 cut(s) 759
Psp6I CCWGG 3 cut(s) 761, 1234, 1804
PspGI CCWGG 3 cut(s) 761, 1234, 1804
PspN4I GGNNCC 8 cut(s) 333, 760, 767, 1038, 1803, 2574, 2797, 3013
PspPI GGNCC 5 cut(s) 759, 765, 1103, 1801, 3131
PspPPI RGGWCCY 1 cut(s) 759
PstI CTGCAG 1 cut(s) 1332
PstNI CAGNNNCTG 1 cut(s) 520
PsuI RGATCY 1 cut(s) 679
PsyI GACNNNGTC 1 cut(s) 763
PteI GCGCGC 1 cut(s) 379
PvuII CAGCTG 4 cut(s) 520, 619, 1242, 3298
RruI TCGCGA 1 cut(s) 421
RsaI GTAC 9 cut(s) 649, 901, 1119, 1198, 1387, 1707, 2010, 2168, 2212
RsaNI GTAC 9 cut(s) 648, 900, 1118, 1197, 1386, 1706, 2009, 2167, 2211
RseI CAYNNNNRTG 4 cut(s) 2715, 2925, 3065, 3221
SaqAI TTAA 9 cut(s) 668, 1940, 1995, 2091, 2148, 2691, 2747, 2915, 2997
Sau3AI GATC 8 cut(s) 438, 507, 679, 2050, 2115, 2902, 3164, 3243
Sau96I GGNCC 5 cut(s) 759, 765, 1103, 1801, 3131
ScaI AGTACT 1 cut(s) 2212
SchI GAGTC 3 cut(s) 25, 938, 1927
ScrFI CCNGG 5 cut(s) 763, 1236, 1636, 1806, 2685
SduI GDGCHC 3 cut(s) 715, 1366, 1847
SexAI ACCWGGT 1 cut(s) 761
SfaNI GCATC 7 cut(s) 1098, 1420, 2475, 2707, 2729, 2975, 3022
SfcI CTRYAG 6 cut(s) 716, 1328, 2536, 2628, 2809, 3114
SfoI GGCGCC 1 cut(s) 2574
Sfr274I CTCGAG 1 cut(s) 1140
SgsI GGCGCGCC 1 cut(s) 379
SinI GGWCC 4 cut(s) 759, 765, 1801, 3131
SlaI CTCGAG 1 cut(s) 1140
SmiMI CAYNNNNRTG 4 cut(s) 2715, 2925, 3065, 3221
SmlI CTYRAG 3 cut(s) 1140, 2347, 2852
SmoI CTYRAG 3 cut(s) 1140, 2347, 2852
SphI GCATGC 1 cut(s) 2957
SseBI AGGCCT 3 cut(s) 107, 2912, 3159
SsiI CCGC 6 cut(s) 190, 799, 825, 851, 2221, 3029
SspDI GGCGCC 1 cut(s) 2572
SspI AATATT 1 cut(s) 1180
StuI AGGCCT 3 cut(s) 107, 2912, 3159
StyD4I CCNGG 5 cut(s) 761, 1234, 1634, 1804, 2683
StyI CCWWGG 6 cut(s) 8, 598, 1573, 2413, 2724, 2799
TaiI ACGT 5 cut(s) 22, 43, 901, 2014, 3337
TaqI TCGA 6 cut(s) 739, 787, 1141, 1586, 2242, 3208
TatI WGTACW 4 cut(s) 1117, 1196, 1705, 2210
TfiI GAWTC 4 cut(s) 2440, 3092, 3119, 3152
Tru1I TTAA 9 cut(s) 668, 1940, 1995, 2091, 2148, 2691, 2747, 2915, 2997
Tru9I TTAA 9 cut(s) 668, 1940, 1995, 2091, 2148, 2691, 2747, 2915, 2997
TscAI CASTG 7 cut(s) 172, 450, 555, 1678, 2379, 2632, 2785
TseFI GTSAC 5 cut(s) 351, 550, 1477, 3101, 3340
Tsp45I GTSAC 5 cut(s) 351, 550, 1477, 3101, 3340
TspGWI ACGGA 4 cut(s) 569, 623, 635, 1507
TspRI CASTG 7 cut(s) 172, 450, 555, 1678, 2379, 2632, 2785
Tth111I GACNNNGTC 1 cut(s) 763
Van91I CCANNNNNTGG 2 cut(s) 100, 1574
VpaK11BI GGWCC 4 cut(s) 759, 765, 1801, 3131
VspI ATTAAT 1 cut(s) 1995
XceI RCATGY 5 cut(s) 472, 1131, 1478, 1975, 2957
XcmI CCANNNNNNNNNTGG 2 cut(s) 1282, 2032
XhoI CTCGAG 1 cut(s) 1140
XmiI GTMKAC 5 cut(s) 560, 1019, 1644, 2448, 2497
XmnI GAANNNNTTC 2 cut(s) 1582, 1610
ZrmI AGTACT 1 cut(s) 2212
Zsp2I ATGCAT 1 cut(s) 2722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.