FvH4_2g35961

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000291
Physical Location & Seq
Forward (+)
26434219 .. 26438778
4560 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g35961.t1

Sequence Viewer

Length: 4245 bp
ATGCCGGGAGGAATGACAAAGCAACAAAAATTTGATTGGTATGACCAAATGCTTGGTGAACTTGCGGGAGTCCCTGACAGATTGATTGACATCACCTCCACGCTAGACCGTGTCGACCTGGATGAACTGGCGACACGAGTCAATGACCTTGGGGAATTGAGAGGGCGAGTTGCAGCCCTAGACCGCGTCGACCTGGATGAGTTGGAGGCGCGAGTCAATGACCTTGAAAGACTGAAAGGGCGAGTTACGGCCCTTGAAAAGATGAAAGCTCCAAGACTCGATGTAGAGACCGAGAGAGATGGAGGTTCTGTTTCACAAGAGCAGATGGGGGCGATGGGTGATGCCCTCGAAACTCTACAAGTGACTATGGACACCATGGCTGAGGATGTCCGAGCCACTATTGATACATTCAAGAACGAGCTGTTGGAGATGAATACCAAGCTCAATCTAGCTATCCGGGCGATGAGTAACCAACCTGTGACGGACTACAGGAGGGTAAAGATCCCGGAACCTCAAGCTTACGGAGGGGCGCGAGACGCCAAGGAGCTTGAGAATTTTCTATTTGATATGGAGCAATACTTCCGAGCAGTGAAGCCGGATTCGGAAGAAGCAAAAGTGGGCATGGCGACAATGTATTTGTTGGGCGATGCGAAACTGTGGTGGAGAACAAAATACAACGACATCCAGAGGAATGTATGTACCATTGCCACATGGGAGGACCTGAAGAAAGAGCTGAAGATGCAGTTCTTCCCAGAGAATGTGGACTACATTGCAAGACGACAATTGAGAGAGCTGAAGCACACCAACAACATTAGAGAGTTTGTGAGCAAATTCTCTGTGCTCATGCTCGATCTCCCAGATATGTCGGAGAAGGACCGACTATTCTATTTCCTCGAGGGGTTAAAGCCGTGGGCTAGAACTGAACTTCAGAGACAAAGGGTTCAAGATTTGGCTTCTGCTCAAGCAGCAGCTGAAAGGTTGACAGACTACACTTTTGAAGAAAACCCTCCCAAAAGGACCTCATCCTTTTCGAATGTGAATGCCAACAAAAGTGTGAGACCAGGACAGAGTAGAAGTGGGGGAGCGGAGTCAAAGTTCTCCAGTTTCGGAGGGGGAAGGATGACCAATGTAAGGGATACAACCTCTTCCAAACCGGCTGCATCAACAGGAGTATTTCCCCCACGACAATTCAACTCCGCCGGCTTCACACCAAGACCTATATCTTGCTTCTTATGCAAGGGGCCTCATAGAGTGAATGAGTGTCCACACAAGACGGCTTTGACAGCCCTACAAGCCCACATTTCGGCGAAGGAGTCTAAGGAACCCCAAGAGGAAGTGGAGGAACCTAGTCAGATGGGTGCTTTGAGATTCTTGAACGCAGTGGAAAAGCAACCACCCGTGTCCCAAGAGCCTCAGCCTAAGGGCCTAATGTTTGTGGATGGGAGCATAAACGGGAAGGTTGCCAAGAGTGTGATGGTTGACACTGGCGCCACCCATAATTTTGTGTCTGAAACAGAAGCACGGAAGTTGGGATTGAAGATAGAGAAGGATACTGGCCGCATGAAAGCAGTCAACTCCTCACCCTTGGCCACAGTGGGGGTGTCTCGAAGAGCACAGATAGTCCCGCCAAGTGAACCCCGTCTCTTGTTTGCTCTCCAGTTCAAGAAAGGGGTGAAGCGACATGAGCCTTCCTACGTCGCTGTCCCCTTGGTAAAAGACAGGGCTGAACCAGAAGTGGTGCCGAAAGAAGTTCAGGGGGTGCTGGAGAGTTACAAGGATGTGATGCCGGCAGATCTCCCCAAGGAGCTGCCACCGAGGAAAAACATAGATCATGAGATTGAGTTACTACCTGGTGCAAAACCACCCGCAAGGGCCCTTTACAGAATGGCTCCGCCAGAGCTAGCAGAATTGAGGAAGCAATTGGGAGATCTGTTAGAAGCAGGGTTCATTCAGCCCTCAAAGGCTCCATTTGGTGCGCCGGTGCTATTCCAAAAGAAGCATGATGGGAGCTTGAGGTTGTGTGTAGACTACCGAGCTCTTAACAAAGTCACCGTGCGGAACAAGTACCCCATTCCCTTGATTGCAGACTTGTTTGACCAGCTTAGTGGTGCCAAATATTTTACCAAGCTGGACCTCCGCTCGGGATACTACCAAGTCCGCATAGCAGAAGGAGATGAGCCCAAGACTGCATGTGTCACCCGATATGGTGCTTTTGAGTTCTTGGTGATGCCGTTCGGGTTGACCAATGCACCTGCCACTTTCTGCACGTTGATGAACCAAGTCTTCCATGGTTACCTTGACAAATTTGTGGTGGTGTATTTGGATGACATTGTGATCTACAGTTCTACTCTGGAGGAACATGTGGAACACCTAAGGCTAGTGTTTCAACAGTTACGAGCCCATCAGCTGTATGTCAAGCGTGAAAAGTGCTCCTTCGCGCAAGATACTATTAAGTTCTTGGGCCATGTTATAGAGAGAGGGAGGATCATGATGGACATGGAGAAAGTACAAGCGATCGAGGAGTGGCAGAGTCCCACGAATGTGAAGGAGCTGCGTTCTTTTCTTGGATTGGCTAATTACTATAGGCGGTTCATTAAGAATTACTCAGGAAGGACAACTCCCTTGACTGACCTCTTGAAGAAGGGAGTGGTGTGGGACTGGAACGAAGACTGTGAGCAAGCCTTCATGAACTTGAAGAAGGCTGTGATGGAAGATCCTGTTCTTGCCTTGCCAGACCTGAGCAAACCATTTGAAGTGCAGACCGATGCATCTGACTTCGCCCTAGGGGGAGTCCTATTACAAGAAGGGCATCCAGTGGCTCATTTCCTGACACAACCGAAGTTGACCTCAAAGCAAGCTCGGTGGCAAGAGTTTCTCGCCGAGTTCGACCTCCAATTTGAGCACAAAGCAGGGTACACCAATCAAGTTGCGGACGCCCTAAGCCGCAAGGCCAACCTTGCGGTCCTTCGAAACCTGGCGACTTTGTCAGGCAGTGGAGTTGCCACTGACATTAAGGAAAGAGTAAAGGCTGGTTTGGAGAAAGATCCCGTGGCTCAAGACCTCGTAAAGCTAGTGCGAGAAGGGAAGAGCCGCAGATTCTGGTTGGAGGACGGCTTGTTAAAGACAAAAGGAGGAAGAACATATCTTCCCCGGGTGGATGGATTGCGAAGAATGTTGATGCGAGAGTGCCATGACACCTTATGGGCAGGGCATCCCGGGTGGCACAGAACCTATGCCCTCCTGAAACAAGGTTACTACTGGCCGCAGATGCGAGACGATATGATGGAGTACACCAGGACGTGTCTCATCTGCCAACAAGACAAGGTAGAACGACAGAAGACAGCTGGGTTGCTGGAGCCGCTGGCAACACCCACTCGTCTATGGAAGAGTGTCTCCCTCGATTTCATTACCAATCTCCCGAAGGTTGGGGAAGTATCTGGAATCCTGGTCGTGGTGGACAGGTTCTCGAAGTATGCAACATTTGTTCCAACATCGAAGCATTGCTCGGCAGAAGACACAGCGAGTCTCTTCTTCAAACATGTGGTGAAGTATTGGGGAGTACCACAAAATATCGTTAGTGATCGAGACCCTAGGTTCACAGGGTCGTTTTGGACAGAGTTGTTCAAGCTACTGGGGTCTGAACTCAACATCTCCTCAAGTTATCACCCTCAGACGGACGGGCAGACTGAGAGATTCAATGCAATGTTGGAAGAATACTTGAGGCACTTTGTTCATGCAAATATGAAGAATTGGGTGGAGTTACTGGATGTGGCCCAGTTCTGTTTCAATTCGAAGAAGAGTTCAGCCACCAACAAGAGCGCGTTTGAAGTGGTGACTGGCCAGCAACCACTCTTGCCTCATACGGTGCAGGAGGTATACAAGGGGAAAAACCCGCGGGCTTTCAACTTCACCAAAGAGTGGAAAGCCAACGCGGACATTGCTCGAAGTTACTTGGAGAAAGCTGCGAGGAGAATGAAGAAGTGGGCAGATCAGAGTCGCAAACCCCGAGAGTTCCGAGAAGGAGACATGGTGCTCGTGAAGCTGCTCCCTGAACAGATCAGGACTATGCGCTCCACAGACAAGCGGTTGTTCAGGAGGTATGAAGGCCCACTTCCGGTCCTTTTGAAAGTGGGGCGGTGCTCTTACAAAGTCGACATCCCTGCCTGGATGAAAGTTCACCTTGTCTTCCATGTCAGCAACCTCAAACCTTACCAGTCTGATGCAGAAGATCAAGCCCGCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asp_protease_2 Chromo RVT_1 Retrotrans_gag gag-asp_proteas rve

Protein Analysis

1415

Amino Acids

161.02

Weight (kDa)

8.95

Isoelectric Point (pI)

40.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ty3_capsid PF19259 169 - 346 4.5e-11 Ty3 transposon capsid-like protein
Retrotrans_gag PF03732 208 - 303 4.3e-15 Retrotransposon gag protein
gag-asp_proteas PF13975 479 - 531 3.1e-07 gag-polyprotein putative aspartyl protease
Asp_protease_2 PF13650 479 - 532 9.9e-07 Aspartyl protease
RVT_1 PF00078 665 - 824 4.5e-39 Reverse transcriptase (RNA-dependent DNA polymerase)
RT_RNaseH_2 PF17919 887 - 942 2.1e-16 RNase H-like domain found in reverse transcriptase
Integrase_H2C2 PF17921 1055 - 1108 4.7e-20 Integrase zinc binding domain
rve PF00665 1138 - 1223 1.9e-06 Integrase core domain
SH3_Tf2-1 PF24626 1341 - 1404 1e-13 Tf2-1-like, SH3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000291)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG00860
fragaria_vesca FvH4_1g23712 FvH4_1g29809 FvH4_1g29810 FvH4_2g02661 FvH4_2g13451 FvH4_2g17921 FvH4_2g17922 FvH4_2g19251 FvH4_2g19252 FvH4_2g28221 FvH4_2g35961 FvH4_3g10372 FvH4_3g12461 FvH4_3g12461 FvH4_3g27861 FvH4_3g34591 FvH4_3g40201 FvH4_3g44612 FvH4_4g08352 FvH4_4g12251 FvH4_4g12742 FvH4_4g13904 FvH4_4g14287 FvH4_4g15243 FvH4_4g24071 FvH4_4g33448 FvH4_5g21912 FvH4_5g39371 FvH4_6g14992 FvH4_6g15221 FvH4_6g20603 FvH4_6g31093 FvH4_6g32372 FvH4_6g33012
malus_domestica MD11G1009100.v1.1 MD13G1145000.v1.1 MD16G1138700.v1.1
prunus_persica Prupe.5G246300_v2.0.a1 Prupe.7G034800_v2.0.a1 Prupe.8G084500_v2.0.a1
pyrus_communis pycom01g18240 pycom04g00330 pycom05g09010 pycom06g01670 pycom06g01690 pycom06g07180 pycom06g11620 pycom10g05720 pycom1134g00040 pycom1134g00060 pycom12486g00010 pycom12555g00230 pycom13g21670 pycom13g23130 pycom13g23140 pycom14g10580
rosa_chinensis RchiOBHm_Chr1g0330691 RchiOBHm_Chr2g0106171 RchiOBHm_Chr2g0134731 RchiOBHm_Chr2g0142791 RchiOBHm_Chr2g0149861 RchiOBHm_Chr3g0452401 RchiOBHm_Chr3g0461881 RchiOBHm_Chr4g0401341 RchiOBHm_Chr5g0075641 RchiOBHm_Chr6g0265961 RchiOBHm_Chr7g0200201 RchiOBHm_Chr7g0237811
rosa_laevigata RLG00000002773
rosa_multiflora Rmu_co8037806.1_g000001 Rmu_co8445989.1_g000001 Rmu_sc0000745.1_g000039 Rmu_sc0004448.1_g000029 Rmu_sc0004488.1_g000002 Rmu_sc0004591.1_g000018 Rmu_sc0005065.1_g000030 Rmu_sc0005150.1_g000005 Rmu_sc0005654.1_g000011 Rmu_sc0006986.1_g000016
rosa_roxburghii Rroxscaffold_164G00436490 Rroxscaffold_175G00432490 Rroxscaffold_1G00059440 Rroxscaffold_1G00064410 Rroxscaffold_2G00100310 Rroxscaffold_2G00128170 Rroxscaffold_3G00239420 Rroxscaffold_3G00264970 Rroxscaffold_3G00271550 Rroxscaffold_4G00300240 Rroxscaffold_5G00385050 Rroxscaffold_6G00399720
rosa_rugosa Rorug02G0252200
rosa_samantha Rh2DG251900 Rh7CG329900
rosa_wichuraiana Rw0G004800 Rw1G000850 Rw1G022350 Rw2G027030 Rw2G027760 Rw2G028410 Rw2G029130 Rw2G029390 Rw2G032490 Rw2G039460 Rw2G047800 Rw2G050340 Rw3G026430 Rw4G018370 Rw4G030750 Rw4G033140 Rw5G011950 Rw5G012010 Rw5G029280 Rw5G040190 Rw5G044570 Rw5G048240 Rw6G000340 Rw6G010140 Rw6G026480 Rw7G033750 Rw7G037250 Rw7G042080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2260
AbsI CCTCGAGG 1 cut(s) 893
Acc36I ACCTGC 1 cut(s) 2260
AccB1I GGYRCC 3 cut(s) 1487, 1738, 2108
AccB7I CCANNNNNTGG 1 cut(s) 3918
AccBSI CCGCTC 2 cut(s) 1085, 2139
AccI GTMKAC 5 cut(s) 114, 189, 2025, 3876, 4152
AccII CGCG 7 cut(s) 186, 211, 532, 2438, 3821, 3895, 3932
AclWI GGATC 4 cut(s) 496, 2492, 2708, 3038
AcoI YGGCCR 4 cut(s) 1555, 1587, 3260, 3838
AcsI RAATTY 4 cut(s) 29, 553, 830, 2303
AcuI CTGAAG 4 cut(s) 743, 755, 815, 911
AcyI GRCGYC 3 cut(s) 537, 1488, 2934
AfaI GTAC 6 cut(s) 700, 2066, 2508, 2915, 3290, 3561
AfiI CCNNNNNNNGG 9 cut(s) 1131, 1303, 1596, 2140, 3425, 3451, 3673, 3918, 4114
AflIII ACRYGT 3 cut(s) 2359, 3299, 3538
AjiI CACGTC 1 cut(s) 3300
AjnI CCWGG 8 cut(s) 117, 192, 1060, 1849, 2973, 3293, 3444, 4163
AjuI GAANNNNNNNTTGG 2 cut(s) 407, 439
AleI CACNNNNGTG 1 cut(s) 2540
AloI GAACNNNNNNTCC 4 cut(s) 1079, 1111, 3130, 3162
Alw21I GWGCWC 7 cut(s) 843, 1615, 2038, 2432, 2904, 4035, 4142
AlwI GGATC 4 cut(s) 496, 2492, 2708, 3038
AlwNI CAGNNNCTG 2 cut(s) 971, 3099
Ama87I CYCGRG 5 cut(s) 893, 2140, 3150, 3215, 4005
ApaI GGGCCC 1 cut(s) 1876
ApeKI GCWGC 8 cut(s) 173, 965, 968, 1157, 1807, 2551, 3962, 4042
ApoI RAATTY 4 cut(s) 29, 553, 830, 2303
ArsI GACNNNNNNTTYG 6 cut(s) 619, 651, 977, 1009, 2879, 2911
AspA2I CCTAGG 2 cut(s) 2782, 3590
AspLEI GCGC 7 cut(s) 211, 532, 1490, 1978, 2440, 3821, 4071
AsuC2I CCSGG 7 cut(s) 6, 458, 506, 3151, 3152, 3216, 3217
AsuII TTCGAA 3 cut(s) 1031, 2968, 3791
AsuNHI GCTAGC 1 cut(s) 1900
AvaI CYCGRG 5 cut(s) 893, 2140, 3150, 3215, 4005
AvaII GGWCC 6 cut(s) 718, 874, 1017, 2131, 2962, 4117
AvrII CCTAGG 2 cut(s) 2782, 3590
AxyI CCTNAGG 2 cut(s) 1419, 2372
BaeGI GKGCMC 1 cut(s) 1876
BalI TGGCCA 2 cut(s) 1589, 3840
BanI GGYRCC 3 cut(s) 1487, 1738, 2108
BanII GRGCYC 4 cut(s) 1876, 2038, 2181, 2401
BauI CACGAG 2 cut(s) 135, 4034
BbsI GAAGAC 5 cut(s) 2275, 2673, 3344, 3519, 4177
Bbv12I GWGCWC 7 cut(s) 843, 1615, 2038, 2432, 2904, 4035, 4142
BbvCI CCTCAGC 2 cut(s) 381, 1413
BbvI GCAGC 8 cut(s) 185, 977, 980, 1144, 1794, 2538, 3949, 4029
BceAI ACGGC 5 cut(s) 264, 892, 1290, 2215, 3127
BcgI CGANNNNNNTGC 2 cut(s) 4142, 4176
BciT130I CCWGG 8 cut(s) 119, 194, 1062, 1851, 2975, 3295, 3446, 4165
BciVI GTATCC 3 cut(s) 1129, 1543, 2138
BcnI CCSGG 7 cut(s) 6, 458, 506, 3151, 3152, 3216, 3217
BfmI CTRYAG 3 cut(s) 487, 2338, 2581
BfoI RGCGCY 1 cut(s) 1491
BfuAI ACCTGC 1 cut(s) 2260
BfuI GTATCC 3 cut(s) 1129, 1543, 2138
BglII AGATCT 2 cut(s) 1792, 1927
BlnI CCTAGG 2 cut(s) 2782, 3590
Bme18I GGWCC 6 cut(s) 718, 874, 1017, 2131, 2962, 4117
BmeT110I CYCGRG 5 cut(s) 893, 2140, 3150, 3215, 4005
BmgBI CACGTC 1 cut(s) 3300
BmrI ACTGGG 2 cut(s) 3641, 3769
BmtI GCTAGC 1 cut(s) 1904
BmuI ACTGGG 2 cut(s) 3641, 3769
BoxI GACNNNNGTC 1 cut(s) 137
BpiI GAAGAC 5 cut(s) 2275, 2673, 3344, 3519, 4177
BpmI CTGGAG 5 cut(s) 1084, 1640, 1784, 2372, 3374
Bpu10I CCTNAGC 4 cut(s) 381, 1413, 2738, 2939
Bpu14I TTCGAA 3 cut(s) 1031, 2968, 3791
BpuEI CTTGAG 7 cut(s) 498, 569, 945, 2032, 3039, 3640, 3739
BpuMI CCSGG 7 cut(s) 6, 458, 506, 3151, 3152, 3216, 3217
BsaBI GATNNNNATC 1 cut(s) 89
BsaHI GRCGYC 3 cut(s) 537, 1488, 2934
BsaI GGTCTC 3 cut(s) 281, 1051, 3579
BsaWI WCCGGW 1 cut(s) 4114
BsaXI ACNNNNNCTCC 8 cut(s) 80, 110, 294, 324, 1079, 1109, 4014, 4044
Bsc4I CCNNNNNNNGG 9 cut(s) 1131, 1303, 1596, 2140, 3425, 3451, 3673, 3918, 4114
Bse118I RCCGGY 4 cut(s) 1153, 1199, 1786, 1978
Bse21I CCTNAGG 2 cut(s) 1419, 2372
Bse3DI GCAATG 5 cut(s) 702, 768, 3499, 3708, 3936
Bse8I GATNNNNATC 1 cut(s) 89
BseBI CCWGG 8 cut(s) 119, 194, 1062, 1851, 2975, 3295, 3446, 4165
BseJI GATNNNNATC 1 cut(s) 89
BseLI CCNNNNNNNGG 9 cut(s) 1131, 1303, 1596, 2140, 3425, 3451, 3673, 3918, 4114
BseMI GCAATG 5 cut(s) 702, 768, 3499, 3708, 3936
BseMII CTCAG 6 cut(s) 372, 1427, 2619, 2729, 3678, 3683
BseRI GAGGAG 4 cut(s) 1567, 2534, 3643, 3982
BseSI GKGCMC 1 cut(s) 1876
BseXI GCAGC 8 cut(s) 185, 977, 980, 1144, 1794, 2538, 3949, 4029
BseYI CCCAGC 1 cut(s) 3344
BsgI GTGCAG 3 cut(s) 2248, 2777, 3887
Bsh1236I CGCG 7 cut(s) 186, 211, 532, 2438, 3821, 3895, 3932
Bsh1285I CGRYCG 1 cut(s) 2517
BshNI GGYRCC 3 cut(s) 1487, 1738, 2108
BsiEI CGRYCG 1 cut(s) 2517
BsiHKAI GWGCWC 7 cut(s) 843, 1615, 2038, 2432, 2904, 4035, 4142
BsiHKCI CYCGRG 5 cut(s) 893, 2140, 3150, 3215, 4005
BslFI GGGAC 6 cut(s) 56, 1387, 1607, 1688, 2517, 2669
BslI CCNNNNNNNGG 9 cut(s) 1131, 1303, 1596, 2140, 3425, 3451, 3673, 3918, 4114
BsmBI CGTCTC 3 cut(s) 528, 1646, 3267
BsmFI GGGAC 6 cut(s) 56, 1387, 1607, 1688, 2517, 2669
BsmI GAATGC 1 cut(s) 1045
Bso31I GGTCTC 3 cut(s) 281, 1051, 3579
BsoBI CYCGRG 5 cut(s) 893, 2140, 3150, 3215, 4005
Bsp119I TTCGAA 3 cut(s) 1031, 2968, 3791
Bsp120I GGGCCC 1 cut(s) 1872
Bsp19I CCATGG 2 cut(s) 375, 2287
BspCNI CTCAG 6 cut(s) 373, 1426, 2618, 2730, 3679, 3682
BspFNI CGCG 7 cut(s) 186, 211, 532, 2438, 3821, 3895, 3932
BspHI TCATGA 3 cut(s) 1831, 2487, 2685
BspMI ACCTGC 1 cut(s) 2260
BspOI GCTAGC 1 cut(s) 1904
BspPI GGATC 4 cut(s) 496, 2492, 2708, 3038
BspQI GCTCTTC 2 cut(s) 1603, 3080
BspT104I TTCGAA 3 cut(s) 1031, 2968, 3791
BspT107I GGYRCC 3 cut(s) 1487, 1738, 2108
BspTNI GGTCTC 3 cut(s) 281, 1051, 3579
BsrBI CCGCTC 2 cut(s) 1085, 2139
BsrDI GCAATG 5 cut(s) 702, 768, 3499, 3708, 3936
BsrFI RCCGGY 4 cut(s) 1153, 1199, 1786, 1978
BssAI RCCGGY 4 cut(s) 1153, 1199, 1786, 1978
BssNAI GTATAC 1 cut(s) 3877
BssNI GRCGYC 3 cut(s) 537, 1488, 2934
BssSI CACGAG 2 cut(s) 135, 4034
BssT1I CCWWGG 9 cut(s) 148, 375, 540, 1584, 1707, 1800, 2287, 2782, 3590
Bst1107I GTATAC 1 cut(s) 3877
Bst2BI CACGAG 2 cut(s) 135, 4034
Bst2UI CCWGG 8 cut(s) 119, 194, 1062, 1851, 2975, 3295, 3446, 4165
Bst4CI ACNGT 8 cut(s) 110, 657, 1594, 2053, 2342, 2391, 2672, 3865
Bst6I CTCTTC 6 cut(s) 1150, 1603, 3080, 3380, 3533, 3791
BstACI GRCGYC 3 cut(s) 537, 1488, 2934
BstBI TTCGAA 3 cut(s) 1031, 2968, 3791
BstC8I GCNNGC 9 cut(s) 1201, 1788, 1902, 2679, 2856, 3363, 3842, 3897, 4237
BstDSI CCRYGG 5 cut(s) 375, 908, 2287, 3048, 3893
BstEII GGTNACC 1 cut(s) 2291
BstFNI CGCG 7 cut(s) 186, 211, 532, 2438, 3821, 3895, 3932
BstH2I RGCGCY 1 cut(s) 1491
BstHHI GCGC 7 cut(s) 211, 532, 1490, 1978, 2440, 3821, 4071
BstMCI CGRYCG 1 cut(s) 2517
BstNI CCWGG 8 cut(s) 119, 194, 1062, 1851, 2975, 3295, 3446, 4165
BstNSI RCATGY 3 cut(s) 2193, 2363, 3542
BstPAI GACNNNNGTC 1 cut(s) 137
BstPI GGTNACC 1 cut(s) 2291
BstSFI CTRYAG 3 cut(s) 487, 2338, 2581
BstSLI GKGCMC 1 cut(s) 1876
BstUI CGCG 7 cut(s) 186, 211, 532, 2438, 3821, 3895, 3932
BstV1I GCAGC 8 cut(s) 185, 977, 980, 1144, 1794, 2538, 3949, 4029
BstV2I GAAGAC 5 cut(s) 2275, 2673, 3344, 3519, 4177
BstX2I RGATCY 5 cut(s) 501, 1792, 1927, 2713, 3043
BstXI CCANNNNNNTGG 2 cut(s) 53, 2105
BstYI RGATCY 5 cut(s) 501, 1792, 1927, 2713, 3043
BstZ17I GTATAC 1 cut(s) 3877
Bsu36I CCTNAGG 2 cut(s) 1419, 2372
BsuI GTATCC 3 cut(s) 1129, 1543, 2138
BtgI CCRYGG 5 cut(s) 375, 908, 2287, 3048, 3893
BtgZI GCGATG 3 cut(s) 347, 476, 660
BtrI CACGTC 1 cut(s) 3300
BtsI GCAGTG 3 cut(s) 594, 1386, 2998
BtsIMutI CAGTG 7 cut(s) 594, 1386, 1482, 1599, 2820, 2998, 3003
BveI ACCTGC 1 cut(s) 2260
Cac8I GCNNGC 9 cut(s) 1201, 1788, 1902, 2679, 2856, 3363, 3842, 3897, 4237
CaiI CAGNNNCTG 2 cut(s) 971, 3099
CciI TCATGA 3 cut(s) 1831, 2487, 2685
CfoI GCGC 7 cut(s) 211, 532, 1490, 1978, 2440, 3821, 4071
Cfr10I RCCGGY 4 cut(s) 1153, 1199, 1786, 1978
Cfr42I CCGCGG 1 cut(s) 3896
Cfr9I CCCGGG 2 cut(s) 3150, 3215
CseI GACGC 3 cut(s) 175, 545, 2942
CsiI ACCWGGT 1 cut(s) 1849
Csp6I GTAC 6 cut(s) 699, 2065, 2507, 2914, 3289, 3560
CspCI CAANNNNNGTGG 2 cut(s) 2843, 2878
CviQI GTAC 6 cut(s) 699, 2065, 2507, 2914, 3289, 3560
DinI GGCGCC 1 cut(s) 1489
EaeI YGGCCR 4 cut(s) 1555, 1587, 3260, 3838
Eam1104I CTCTTC 6 cut(s) 1150, 1603, 3080, 3380, 3533, 3791
EarI CTCTTC 6 cut(s) 1150, 1603, 3080, 3380, 3533, 3791
EciI GGCGGA 2 cut(s) 1186, 1881
Ecl136II GAGCTC 1 cut(s) 2036
Eco130I CCWWGG 9 cut(s) 148, 375, 540, 1584, 1707, 1800, 2287, 2782, 3590
Eco24I GRGCYC 4 cut(s) 1876, 2038, 2181, 2401
Eco31I GGTCTC 3 cut(s) 281, 1051, 3579
Eco47I GGWCC 6 cut(s) 718, 874, 1017, 2131, 2962, 4117
Eco53kI GAGCTC 1 cut(s) 2036
Eco57I CTGAAG 4 cut(s) 743, 755, 815, 911
Eco81I CCTNAGG 2 cut(s) 1419, 2372
Eco88I CYCGRG 5 cut(s) 893, 2140, 3150, 3215, 4005
Eco91I GGTNACC 1 cut(s) 2291
EcoICRI GAGCTC 1 cut(s) 2036
EcoO109I RGGNCCY 6 cut(s) 718, 1017, 1241, 1423, 1872, 1873
EcoO65I GGTNACC 1 cut(s) 2291
EcoRII CCWGG 8 cut(s) 117, 192, 1060, 1849, 2973, 3293, 3444, 4163
EcoT14I CCWWGG 9 cut(s) 148, 375, 540, 1584, 1707, 1800, 2287, 2782, 3590
EcoT22I ATGCAT 1 cut(s) 2770
EcoT38I GRGCYC 4 cut(s) 1876, 2038, 2181, 2401
EgeI GGCGCC 1 cut(s) 1489
EheI GGCGCC 1 cut(s) 1489
ErhI CCWWGG 9 cut(s) 148, 375, 540, 1584, 1707, 1800, 2287, 2782, 3590
Esp3I CGTCTC 3 cut(s) 528, 1646, 3267
FalI AAGNNNNNCTT 6 cut(s) 2417, 2449, 4095, 4127, 4164, 4196
FaqI GGGAC 6 cut(s) 56, 1387, 1607, 1688, 2517, 2669
FauI CCCGC 5 cut(s) 58, 1632, 1873, 3888, 3900
FblI GTMKAC 5 cut(s) 114, 189, 2025, 3876, 4152
FriOI GRGCYC 4 cut(s) 1876, 2038, 2181, 2401
GlaI GCGC 7 cut(s) 210, 531, 1489, 1977, 2439, 3820, 4070
GsaI CCCAGC 1 cut(s) 3348
GsuI CTGGAG 5 cut(s) 1084, 1640, 1784, 2372, 3374
HaeII RGCGCY 1 cut(s) 1491
HgaI GACGC 3 cut(s) 175, 545, 2942
HhaI GCGC 7 cut(s) 211, 532, 1490, 1978, 2440, 3821, 4071
Hin1I GRCGYC 3 cut(s) 537, 1488, 2934
Hin6I GCGC 7 cut(s) 209, 530, 1488, 1976, 2438, 3819, 4069
HinP1I GCGC 7 cut(s) 209, 530, 1488, 1976, 2438, 3819, 4069
HincII GTYRAC 8 cut(s) 115, 190, 981, 1480, 1573, 2241, 2844, 4153
HindII GTYRAC 8 cut(s) 115, 190, 981, 1480, 1573, 2241, 2844, 4153
HindIII AAGCTT 1 cut(s) 516
Hpy99I CGWCG 2 cut(s) 191, 1700
HpyCH4III ACNGT 8 cut(s) 110, 657, 1594, 2053, 2342, 2391, 2672, 3865
HpyCH4IV ACGT 3 cut(s) 1695, 2267, 3299
HpySE526I ACGT 3 cut(s) 1695, 2267, 3299
Hsp92I GRCGYC 3 cut(s) 537, 1488, 2934
HspAI GCGC 7 cut(s) 209, 530, 1488, 1976, 2438, 3819, 4069
KasI GGCGCC 1 cut(s) 1487
KroI GCCGGC 2 cut(s) 1199, 1786
KroNI GCCGGC 2 cut(s) 1201, 1788
KspI CCGCGG 1 cut(s) 3896
LguI GCTCTTC 2 cut(s) 1603, 3080
Lsp1109I GCAGC 8 cut(s) 185, 977, 980, 1144, 1794, 2538, 3949, 4029
MabI ACCWGGT 1 cut(s) 1849
MaeII ACGT 3 cut(s) 1695, 2267, 3299
MbiI CCGCTC 2 cut(s) 1085, 2139
MfeI CAATTG 2 cut(s) 782, 1919
MflI RGATCY 5 cut(s) 501, 1792, 1927, 2713, 3043
MlsI TGGCCA 2 cut(s) 1589, 3840
MluNI TGGCCA 2 cut(s) 1589, 3840
Mly113I GGCGCC 1 cut(s) 1488
MmeI TCCRAC 6 cut(s) 183, 405, 846, 3084, 3512, 3687
Mox20I TGGCCA 2 cut(s) 1589, 3840
Mph1103I ATGCAT 1 cut(s) 2770
MroNI GCCGGC 2 cut(s) 1199, 1786
MscI TGGCCA 2 cut(s) 1589, 3840
MseI TTAA 6 cut(s) 902, 2040, 2451, 2595, 3012, 3119
MslI CAYNNNNRTG 2 cut(s) 2270, 2540
Msp20I TGGCCA 2 cut(s) 1589, 3840
MspA1I CMGCKG 5 cut(s) 971, 2407, 3344, 3361, 3895
MunI CAATTG 2 cut(s) 782, 1919
Mva1269I GAATGC 1 cut(s) 1045
MvaI CCWGG 8 cut(s) 119, 194, 1062, 1851, 2975, 3295, 3446, 4165
MvnI CGCG 7 cut(s) 186, 211, 532, 2438, 3821, 3895, 3932
NaeI GCCGGC 2 cut(s) 1201, 1788
NarI GGCGCC 1 cut(s) 1488
NciI CCSGG 7 cut(s) 6, 458, 506, 3151, 3152, 3216, 3217
NcoI CCATGG 2 cut(s) 375, 2287
NgoMIV GCCGGC 2 cut(s) 1199, 1786
NheI GCTAGC 1 cut(s) 1900
NmeAIII GCCGAG 2 cut(s) 2905, 3485
NmuCI GTSAC 5 cut(s) 361, 478, 2047, 2194, 3832
NsiI ATGCAT 1 cut(s) 2770
NspI RCATGY 3 cut(s) 2193, 2363, 3542
NspV TTCGAA 3 cut(s) 1031, 2968, 3791
OliI CACNNNNGTG 1 cut(s) 2540
PaeR7I CTCGAG 1 cut(s) 893
PagI TCATGA 3 cut(s) 1831, 2487, 2685
PaqCI CACCTGC 1 cut(s) 2260
PciI ACATGT 2 cut(s) 2359, 3538
PciSI GCTCTTC 2 cut(s) 1603, 3080
PcsI WCGNNNNNNNCGW 2 cut(s) 2883, 4003
PctI GAATGC 1 cut(s) 1045
PdiI GCCGGC 2 cut(s) 1201, 1788
PfeI GAWTC 5 cut(s) 599, 1368, 3096, 3441, 3693
PflFI GACNNNGTC 3 cut(s) 110, 185, 2983
PflMI CCANNNNNTGG 1 cut(s) 3918
PfoI TCCNGGA 1 cut(s) 504
Ple19I CGATCG 1 cut(s) 2517
PluTI GGCGCC 1 cut(s) 1491
PpuMI RGGWCCY 2 cut(s) 718, 1017
PscI ACATGT 2 cut(s) 2359, 3538
PshAI GACNNNNGTC 1 cut(s) 137
Psp124BI GAGCTC 1 cut(s) 2038
Psp5II RGGWCCY 2 cut(s) 718, 1017
Psp6I CCWGG 8 cut(s) 117, 192, 1060, 1849, 2973, 3293, 3444, 4163
PspEI GGTNACC 1 cut(s) 2291
PspFI CCCAGC 1 cut(s) 3344
PspGI CCWGG 8 cut(s) 117, 192, 1060, 1849, 2973, 3293, 3444, 4163
PspOMI GGGCCC 1 cut(s) 1872
PspPPI RGGWCCY 2 cut(s) 718, 1017
PspXI VCTCGAGB 1 cut(s) 893
PstNI CAGNNNCTG 2 cut(s) 971, 3099
PsuI RGATCY 5 cut(s) 501, 1792, 1927, 2713, 3043
PsyI GACNNNGTC 3 cut(s) 110, 185, 2983
PvuI CGATCG 1 cut(s) 2517
PvuII CAGCTG 3 cut(s) 971, 2407, 3344
RsaI GTAC 6 cut(s) 700, 2066, 2508, 2915, 3290, 3561
RsaNI GTAC 6 cut(s) 699, 2065, 2507, 2914, 3289, 3560
RseI CAYNNNNRTG 2 cut(s) 2270, 2540
SacI GAGCTC 1 cut(s) 2038
SacII CCGCGG 1 cut(s) 3896
SalI GTCGAC 3 cut(s) 113, 188, 4151
SapI GCTCTTC 2 cut(s) 1603, 3080
SaqAI TTAA 6 cut(s) 902, 2040, 2451, 2595, 3012, 3119
SexAI ACCWGGT 1 cut(s) 1849
SfcI CTRYAG 3 cut(s) 487, 2338, 2581
SfoI GGCGCC 1 cut(s) 1489
Sfr274I CTCGAG 1 cut(s) 893
Sfr303I CCGCGG 1 cut(s) 3896
SfuI TTCGAA 3 cut(s) 1031, 2968, 3791
SgrAI CRCCGGYG 1 cut(s) 1978
SgrBI CCGCGG 1 cut(s) 3896
SinI GGWCC 6 cut(s) 718, 874, 1017, 2131, 2962, 4117
SlaI CTCGAG 1 cut(s) 893
SmaI CCCGGG 2 cut(s) 3152, 3217
SmiMI CAYNNNNRTG 2 cut(s) 2270, 2540
SmlI CTYRAG 8 cut(s) 513, 548, 893, 960, 2011, 3054, 3655, 3718
SmoI CTYRAG 8 cut(s) 513, 548, 893, 960, 2011, 3054, 3655, 3718
SspDI GGCGCC 1 cut(s) 1487
SspI AATATT 1 cut(s) 2117
SstI GAGCTC 1 cut(s) 2038
StyI CCWWGG 9 cut(s) 148, 375, 540, 1584, 1707, 1800, 2287, 2782, 3590
TaaI ACNGT 8 cut(s) 110, 657, 1594, 2053, 2342, 2391, 2672, 3865
TaiI ACGT 3 cut(s) 1698, 2270, 3302
TaqII GACCGA 3 cut(s) 305, 891, 2777
TatI WGTACW 2 cut(s) 2506, 3288
TauI GCSGC 5 cut(s) 1560, 2946, 3093, 3265, 3361
TfiI GAWTC 5 cut(s) 599, 1368, 3096, 3441, 3693
Tru1I TTAA 6 cut(s) 902, 2040, 2451, 2595, 3012, 3119
Tru9I TTAA 6 cut(s) 902, 2040, 2451, 2595, 3012, 3119
TscAI CASTG 7 cut(s) 594, 1386, 1489, 1599, 2820, 2998, 3010
TseFI GTSAC 5 cut(s) 361, 478, 2047, 2194, 3832
TseI GCWGC 8 cut(s) 173, 965, 968, 1157, 1807, 2551, 3962, 4042
Tsp45I GTSAC 5 cut(s) 361, 478, 2047, 2194, 3832
TspGWI ACGGA 4 cut(s) 497, 537, 1537, 3689
TspMI CCCGGG 2 cut(s) 3150, 3215
TspRI CASTG 7 cut(s) 594, 1386, 1489, 1599, 2820, 2998, 3010
Tth111I GACNNNGTC 3 cut(s) 110, 185, 2983
Van91I CCANNNNNTGG 1 cut(s) 3918
VpaK11BI GGWCC 6 cut(s) 718, 874, 1017, 2131, 2962, 4117
XapI RAATTY 4 cut(s) 29, 553, 830, 2303
XceI RCATGY 3 cut(s) 2193, 2363, 3542
XcmI CCANNNNNNNNNTGG 2 cut(s) 1471, 2285
XhoI CTCGAG 1 cut(s) 893
XmaI CCCGGG 2 cut(s) 3150, 3215
XmaJI CCTAGG 2 cut(s) 2782, 3590
XmiI GTMKAC 5 cut(s) 114, 189, 2025, 3876, 4152
Zsp2I ATGCAT 1 cut(s) 2770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.