Rw6G026480
ERF Family

DNA RNA polymerases superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000291
Physical Location & Seq
Forward (+)
49891442 .. 49895374
3933 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G026480.1

Sequence Viewer

Length: 3933 bp
ATGAAGAAGGAGTTGAAGAAGAAGTTTCTACCTGAAAATTACCTACAAAGTAATTTTTTGAAGCTACACAACATTCGACAGGGGAATCGGTCTGTGGATGAGTTTACTGAGGAGTTTGATCTTCTTACCATGAGATGTGGTATGATTGAAGAGGAAGAGCAGATAGTAGCACGATATCTGGCAGGGCTGAGGCGTGAGATCCATGATGTTGTGGTGCTACAACCATGCTGGAGTTACAGTGATGTGTACCAATTGGCAATCCAAGTGGAGAAGCAACAACGAGTACGTTACAAGCGGGGAGCAAGTGAAGATTATGAACACAAAAGAATAACTTCACCAAGTGCTCCCAAAACCACACCTATTATTGATTCTAACAGACGTGAACCAATAAAGAACCAAGCTGAACATAAGACAGAAGCTAGGGAGGGCAACAAGGCCAAAAATGTGAAATGCTTCAAGTGCTCAGGCCTAGGGCACATTGCATCTGATTGTCCCAACCGAAGGATTGTGAGTTTGGTGGAGGAATTAGGGGAGAGCAGTAGCGTGAGGTTGGACGACCCACCCACCTATGATGACTATGGGGACCAAGATGCAGAAGATATAACCTGGAGCGATCATGGGGACTCACTAGTTATTCGGCGGGCAATGAATACAAAAAAGGTTGATGATGATTCTGAGTGGCTCCGGCACAACATCTTTCACACTAAGTGTACCGCGAATGGCAAGGTATGTAGTGTTATTATTGATAGTGGGAGTTGTGAAAATATTGTTGCTCAACAAATGGTGGATAAGTTAAATCTGATAACTGAGAGAAGGCCTAAATCTTACAAGTTGTCTTGGTTCAAGAAGGGAAATGATGTTGTTGTCAACCAGAGGTGTTTGGTGTGTTTTTCTATTGGCACTAAGTACCAAGACTCACAGTGGTGTGATGTAGCTCCCATGGATGCTTGTCATCTTTTGTTAGGGCGTCCATGGCAATATGATCGGAAGGCTGTACATGATGGGCATCTAAACACCTACTCCTTTGTCAAGGATGGTAACAAAATTATTTTGGGACCATCTAGGTCAGAGCACAAGCCTAAATCAGATCAGGCAGAAGGTGGCAATCTTCTCACTAGGTGCAAATTTTTGAATGACGCAAGAGAGAGGGGTGTGGTCTATATGCTTATGGGAAGAGAAGCTGATGATACTGAACTTAATCACCCTGAAGTAGTAGCTTCATTATTGAGAGAATTTGCTGATATTGTTCCAGAAGAGTTGCCTGGAGGATTACCCCCTTTGAGGGACATCCAACACCACATTGATTTTGTACCAGGAGCAAGCTTGCCAAACAAACCTCATTATCGAATGAGTCCTCAAGAATATGATGAGCTGAATAAGCAAGTGACAGAGTTGTTGGAGAAAGGAGTTATCCGGGAGAGTATGAGCCCTTGCGCGGTGCCGGCTTTACTTACACCTAAAAAAGATGGTACTTGGCGAATGTGTGTGGATAGTAGGGCTATAAACAAAATTACCGTGCGGTATAGATTCCCAATTCCACGCTTGGAAGACATGTTAGATCACTTGTCAAGTGCCAAAGTATTCTCAAAGATTGATCTGAGGAGTGGATACCACCAGATAAGAATGAGGCCTGGGGATGAGTGGAAGACCGCATTTAAAACCCGAGATGGCTTGTTTGAATGGATGGTGATGCCATTTGGGCTTACGAATGCCCCTAGCACATTTATGCGTGTCATGAACCAGGTATTTCGTTCTTTCATTGGCAAGTTTGTGGTTGTGTATTTCGATGATATTCTGGTTTATAGCATTGATGTTAATCAACATATGGAACACTTGAGACAAGTCTTTGAAGTATTGAGGGAGCAGAAGCTTTATGCAAATTTCAAGAAGTGTGAGTTCTTAACAAACAAATTAATTTTTTTGGGTTTTATTATCTCCACTGATGGAGTACAAGTGGATCCAAAAAAAATTGAAGCAATCTTGGAATGGCCTAATCCTCAATCTATTCATGATGTTCGAAGCTTTCATGGGTTGGCTTCCTTTTATAGAAGATTCATCAAGAATTTCAGTTCCATTGTGGCTCCAATGACAGATTGTTTAAAGGCCAGTGTGTTTAAGTGGACAGATGCAGCTGAAAGGAGCTTTCAAGCAATTAAATTAGCCATGACAGAGGCTCCTGTGTTGGCTCTTCCAGACTACCAAAAGGTCTTTGAAGTTGATTGTGACGCTTCCAAGTTCGGGGTTGGAGCGGTTCTAAGTCAAGAAGGTAGGCCAATTGCGTTCTATAGTGAAAAACTCAATGAAGCCAGACAGCGATACTCGACCTATGATGTTGAATTCTATGCTATCATGCAAGCTCTTCGGCAATGGAGGCAGTATCTTATTTATAAAGAATTTATTCTTTATTCTGATCATGAAGCACTCAAGTTTATCAATGGTCAACAAAAGTTGAACAGGCGACATGCAAAGTGGGTTGAAGAGCTGCAAGAGTATGATTTTGTAATCAAACACAAGTCAGGGATTCAAAACAAAGTAGCTGATGCTTTGAGTAGGCGGCAAACTTTGTTATCTCTCATGAAGGTAAGAGTAGAAGGTTTTGAACAGCTGAGAGAGCTCTATGGAGTAGATGCTGATTTTTGTGATGTGTGGGGCAATTGTACCAGCAAGGGACCACAACAGGATTTTCATGTCCAAGACGGTTATTTGTTTAAAGGGAGTAGATTGTGTATCCCTGAGAGTTCTTTGCGTTTCTGGATAATTGAAGAACAACATGGTGGTGGTTTAGGTGGGCATTTTGGACGGGACAAGACTTTGGCATTAGTGAGTGAGAAGTTTTATTGGCCAAAGTTACAAAAGGATGTGATGAAATATGTTCAGCGGTGTCGAACTTGTCATCTAGCCAAGAGCCGAAGTCAGAATACTGGGCTCTACACTCCTTTGCCTGAACCCCAAGCTCCATGGGTGGATGTCAGCATGGATTTCGTTGTAGGACTTCCAAGAACAAGAAATGGACATGATTCAATATTGGTGGTGGTTGACCGTTTTTCGAAGATGGCACACTTCGTGCCTTGCAAGAAAGCCATGGATGCAAGTCACATTGCAAATCTTTATTTTCGGGAGATTGTTAAGCTGCATGGAATCCCCTCTACTATTACTTCAGATAGAGATACAAAGTTTGTGAGTCACTTTTGGAGGACATTGTGGCGTAAGCTTGGAACTAAGTTGCAATTCAGTAGTGCTCATCATCCTCAGACAGATGGCCAAACTGAAGTAGTTAATAGAACTTTGGGTAACTTACTAAGGTCTCTGGTGGGGAGTAACAAGGGCAGTTGGTGTGAAGTTCTCCCTCAAGCTGAGTTTGCTTATAATAGATCCAGGCATAGGAGCATCGAGATGAGTCCTTTTGAGGCTGTATATGGGCACAATCCTAATAGCCCCATGGATTTAATTCCACTACCAGTTGTTGACAGGTTCAGTGGAGATGCACAAGATATGGTACATCACATCAAGAAGCTTCACGAGACCGTACGGCGGAGATTGGAAGCAAGTAATTTAAAGTACAAAGAGCATGCTGATCAACATAGGCGCCAAGTTGAATTCAAAGTGGGTGACTTGGTATGGATAAATTTGAGGAAAGAAAGGTTCCCAAGAGGAAAATATGGAAAACTACATGATCGAGGTGGAGGACCATACAAGGTACTGAAGAAGGCCGGATCAAATGCGTACGTGCTTGAACTGCCTGCTGATATTGGTGTCTCTCCTACTTTCAATGTGTCTGATTTGCAAGCCTATCATGGGGAGAATGATCTCATTGTGACTGACTCGAGGGCGAGTCCTTCTCAACCCGGAGAGTCTGATGCAGGGGCGTCTACTCTAAAACCAAAGAGAAAGACCCAAGCTCCACCTTACCTCAAAGACTTTGTGCCCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 Retrotrans_gag rve zf-CCHC zf-H2C2

Protein Analysis

1310

Amino Acids

150.61

Weight (kDa)

8.83

Isoelectric Point (pI)

47.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotrans_gag PF03732 1 - 65 5e-09 Retrotransposon gag protein
RVT_1 PF00078 486 - 645 5.3e-40 Reverse transcriptase (RNA-dependent DNA polymerase)
RT_RNaseH_2 PF17919 707 - 800 6e-30 RNase H-like domain found in reverse transcriptase
RT_RNaseH PF17917 732 - 831 1.6e-32 RNase H-like domain found in reverse transcriptase
Integrase_H2C2 PF17921 913 - 970 3.2e-19 Integrase zinc binding domain
rve PF00665 990 - 1083 2.1e-09 Integrase core domain
SH3_Tf2-1 PF24626 1203 - 1265 9.6e-19 Tf2-1-like, SH3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000291)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG00860
fragaria_vesca FvH4_1g23712 FvH4_1g29809 FvH4_1g29810 FvH4_2g02661 FvH4_2g13451 FvH4_2g17921 FvH4_2g17922 FvH4_2g19251 FvH4_2g19252 FvH4_2g28221 FvH4_2g35961 FvH4_3g10372 FvH4_3g12461 FvH4_3g12461 FvH4_3g27861 FvH4_3g34591 FvH4_3g40201 FvH4_3g44612 FvH4_4g08352 FvH4_4g12251 FvH4_4g12742 FvH4_4g13904 FvH4_4g14287 FvH4_4g15243 FvH4_4g24071 FvH4_4g33448 FvH4_5g21912 FvH4_5g39371 FvH4_6g14992 FvH4_6g15221 FvH4_6g20603 FvH4_6g31093 FvH4_6g32372 FvH4_6g33012
malus_domestica MD11G1009100.v1.1 MD13G1145000.v1.1 MD16G1138700.v1.1
prunus_persica Prupe.5G246300_v2.0.a1 Prupe.7G034800_v2.0.a1 Prupe.8G084500_v2.0.a1
pyrus_communis pycom01g18240 pycom04g00330 pycom05g09010 pycom06g01670 pycom06g01690 pycom06g07180 pycom06g11620 pycom10g05720 pycom1134g00040 pycom1134g00060 pycom12486g00010 pycom12555g00230 pycom13g21670 pycom13g23130 pycom13g23140 pycom14g10580
rosa_chinensis RchiOBHm_Chr1g0330691 RchiOBHm_Chr2g0106171 RchiOBHm_Chr2g0134731 RchiOBHm_Chr2g0142791 RchiOBHm_Chr2g0149861 RchiOBHm_Chr3g0452401 RchiOBHm_Chr3g0461881 RchiOBHm_Chr4g0401341 RchiOBHm_Chr5g0075641 RchiOBHm_Chr6g0265961 RchiOBHm_Chr7g0200201 RchiOBHm_Chr7g0237811
rosa_laevigata RLG00000002773
rosa_multiflora Rmu_co8037806.1_g000001 Rmu_co8445989.1_g000001 Rmu_sc0000745.1_g000039 Rmu_sc0004448.1_g000029 Rmu_sc0004488.1_g000002 Rmu_sc0004591.1_g000018 Rmu_sc0005065.1_g000030 Rmu_sc0005150.1_g000005 Rmu_sc0005654.1_g000011 Rmu_sc0006986.1_g000016
rosa_roxburghii Rroxscaffold_164G00436490 Rroxscaffold_175G00432490 Rroxscaffold_1G00059440 Rroxscaffold_1G00064410 Rroxscaffold_2G00100310 Rroxscaffold_2G00128170 Rroxscaffold_3G00239420 Rroxscaffold_3G00264970 Rroxscaffold_3G00271550 Rroxscaffold_4G00300240 Rroxscaffold_5G00385050 Rroxscaffold_6G00399720
rosa_rugosa Rorug02G0252200
rosa_samantha Rh2DG251900 Rh7CG329900
rosa_wichuraiana Rw0G004800 Rw1G000850 Rw1G022350 Rw2G027030 Rw2G027760 Rw2G028410 Rw2G029130 Rw2G029390 Rw2G032490 Rw2G039460 Rw2G047800 Rw2G050340 Rw3G026430 Rw4G018370 Rw4G030750 Rw4G033140 Rw5G011950 Rw5G012010 Rw5G029280 Rw5G040190 Rw5G044570 Rw5G048240 Rw6G000340 Rw6G010140 Rw6G026480 Rw7G033750 Rw7G037250 Rw7G042080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2388, 3366
AccB1I GGYRCC 2 cut(s) 1438, 3585
AccBSI CCGCTC 1 cut(s) 2249
AccI GTMKAC 1 cut(s) 3869
AccII CGCG 2 cut(s) 716, 1436
AclWI GGATC 5 cut(s) 193, 1952, 1965, 3366, 3721
AcoI YGGCCR 2 cut(s) 2840, 3259
AcsI RAATTY 8 cut(s) 1124, 1232, 1879, 2062, 2336, 2393, 3596, 3625
AcuI CTGAAG 4 cut(s) 1227, 3141, 3288, 3722
AcyI GRCGYC 3 cut(s) 967, 3586, 3866
AdeI CACNNNGTG 4 cut(s) 341, 708, 1119, 3064
AfiI CCNNNNNNNGG 8 cut(s) 501, 1281, 1282, 1435, 2238, 3381, 3531, 3795
AflIII ACRYGT 1 cut(s) 1551
AhlI ACTAGT 1 cut(s) 628
AjiI CACGTC 1 cut(s) 380
AjnI CCWGG 6 cut(s) 605, 1261, 1312, 1630, 1740, 3374
AleI CACNNNNGTG 1 cut(s) 922
Alw21I GWGCWC 5 cut(s) 346, 464, 1074, 2616, 3241
Alw26I GTCTC 4 cut(s) 1831, 3309, 3515, 3760
AlwI GGATC 5 cut(s) 193, 1952, 1965, 3366, 3721
Ama87I CYCGRG 2 cut(s) 1662, 3823
ApeKI GCWGC 3 cut(s) 2129, 2482, 3130
ApoI RAATTY 8 cut(s) 1124, 1232, 1879, 2062, 2336, 2393, 3596, 3625
AseI ATTAAT 1 cut(s) 1913
Asp700I GAANNNNTTC 3 cut(s) 331, 452, 2397
AspA2I CCTAGG 1 cut(s) 469
AspLEI GCGC 2 cut(s) 1436, 3588
AspS9I GGNCC 4 cut(s) 583, 1055, 2669, 3686
AsuC2I CCSGG 2 cut(s) 1415, 3846
AsuHPI GGTGA 4 cut(s) 327, 1193, 1699, 3620
AsuII TTCGAA 2 cut(s) 2017, 3047
AvaI CYCGRG 2 cut(s) 1662, 3823
AvaII GGWCC 4 cut(s) 583, 1055, 2669, 3686
AvrII CCTAGG 1 cut(s) 469
BaeGI GKGCMC 3 cut(s) 477, 3423, 3927
BalI TGGCCA 2 cut(s) 2842, 3261
BamHI GGATCC 1 cut(s) 1957
BanI GGYRCC 2 cut(s) 1438, 3585
BanII GRGCYC 3 cut(s) 1430, 2616, 2928
BarI GAAGNNNNNNTAC 2 cut(s) 2902, 2934
BauI CACGAG 1 cut(s) 3518
BbsI GAAGAC 2 cut(s) 1554, 1652
Bbv12I GWGCWC 5 cut(s) 346, 464, 1074, 2616, 3241
BbvCI CCTCAGC 1 cut(s) 188
BbvI GCAGC 3 cut(s) 2141, 2469, 3117
BccI CCATC 9 cut(s) 995, 1028, 1066, 1460, 1661, 1678, 1937, 3046, 3251
BceAI ACGGC 1 cut(s) 3545
BcgI CGANNNNNNTGC 6 cut(s) 965, 999, 2342, 2376, 2962, 2996
BciT130I CCWGG 6 cut(s) 607, 1263, 1314, 1632, 1742, 3376
BciVI GTATCC 2 cut(s) 1601, 2738
BclI TGATCA 2 cut(s) 2410, 3574
BcnI CCSGG 2 cut(s) 1415, 3846
BcoDI GTCTC 4 cut(s) 1831, 3309, 3515, 3760
BcuI ACTAGT 1 cut(s) 628
BfaI CTAG 7 cut(s) 420, 470, 629, 1062, 1116, 1716, 2897
BfmI CTRYAG 1 cut(s) 2284
BfoI RGCGCY 1 cut(s) 3589
BfuI GTATCC 2 cut(s) 1601, 2738
BglI GCCNNNNNGGC 1 cut(s) 1699
BisI GCNGC 4 cut(s) 2130, 2483, 2555, 3131
BlnI CCTAGG 1 cut(s) 469
BlsI GCNGC 4 cut(s) 2131, 2484, 2556, 3132
Bme1390I CCNGG 8 cut(s) 607, 1263, 1314, 1415, 1632, 1742, 3376, 3846
Bme18I GGWCC 4 cut(s) 583, 1055, 2669, 3686
BmeT110I CYCGRG 2 cut(s) 1662, 3823
BmgBI CACGTC 1 cut(s) 380
BmgT120I GGNCC 4 cut(s) 583, 1055, 2669, 3686
BmrFI CCNGG 8 cut(s) 607, 1263, 1314, 1415, 1632, 1742, 3376, 3846
BmrI ACTGGG 1 cut(s) 2931
BmuI ACTGGG 1 cut(s) 2931
BpiI GAAGAC 2 cut(s) 1554, 1652
BplI GAGNNNNNCTC 2 cut(s) 3823, 3855
BpmI CTGGAG 3 cut(s) 250, 628, 1284
Bpu10I CCTNAGC 2 cut(s) 188, 463
Bpu14I TTCGAA 2 cut(s) 2017, 3047
BpuEI CTTGAG 4 cut(s) 1341, 1855, 2408, 3333
BpuMI CCSGG 2 cut(s) 1415, 3846
BsaAI YACGTR 1 cut(s) 3727
BsaBI GATNNNNATC 2 cut(s) 1005, 1815
BsaHI GRCGYC 3 cut(s) 967, 3586, 3866
BsaI GGTCTC 2 cut(s) 3309, 3515
BsaJI CCNNGG 7 cut(s) 469, 939, 971, 1631, 2957, 3081, 3438
Bsc4I CCNNNNNNNGG 8 cut(s) 501, 1281, 1282, 1435, 2238, 3381, 3531, 3795
Bse118I RCCGGY 1 cut(s) 1441
Bse1I ACTGG 3 cut(s) 2106, 2926, 3458
Bse3DI GCAATG 4 cut(s) 477, 651, 2372, 3096
Bse8I GATNNNNATC 2 cut(s) 1005, 1815
BseBI CCWGG 6 cut(s) 607, 1263, 1314, 1632, 1742, 3376
BseDI CCNNGG 7 cut(s) 469, 939, 971, 1631, 2957, 3081, 3438
BseJI GATNNNNATC 2 cut(s) 1005, 1815
BseLI CCNNNNNNNGG 8 cut(s) 501, 1281, 1282, 1435, 2238, 3381, 3531, 3795
BseMI GCAATG 4 cut(s) 477, 651, 2372, 3096
BseNI ACTGG 3 cut(s) 2106, 2926, 3458
BseRI GAGGAG 2 cut(s) 125, 1615
BseSI GKGCMC 3 cut(s) 477, 3423, 3927
BseXI GCAGC 3 cut(s) 2141, 2469, 3117
Bsh1236I CGCG 2 cut(s) 716, 1436
BshNI GGYRCC 2 cut(s) 1438, 3585
BsiHKAI GWGCWC 5 cut(s) 346, 464, 1074, 2616, 3241
BsiHKCI CYCGRG 2 cut(s) 1662, 3823
BsiSI CCGG 5 cut(s) 685, 1414, 1442, 3711, 3846
BsiWI CGTACG 2 cut(s) 3526, 3723
BslFI GGGAC 7 cut(s) 477, 596, 635, 1068, 1298, 2682, 2816
BslI CCNNNNNNNGG 8 cut(s) 501, 1281, 1282, 1435, 2238, 3381, 3531, 3795
BsmAI GTCTC 4 cut(s) 1831, 3309, 3515, 3760
BsmFI GGGAC 7 cut(s) 477, 596, 635, 1068, 1298, 2682, 2816
BsmI GAATGC 1 cut(s) 1714
Bso31I GGTCTC 2 cut(s) 3309, 3515
BsoBI CYCGRG 2 cut(s) 1662, 3823
Bsp119I TTCGAA 2 cut(s) 2017, 3047
Bsp1407I TGTACA 1 cut(s) 994
Bsp19I CCATGG 5 cut(s) 939, 971, 2957, 3081, 3438
BspFNI CGCG 2 cut(s) 716, 1436
BspHI TCATGA 4 cut(s) 1734, 2008, 2413, 2574
BspPI GGATC 5 cut(s) 193, 1952, 1965, 3366, 3721
BspQI GCTCTTC 4 cut(s) 150, 2193, 2364, 2472
BspT104I TTCGAA 2 cut(s) 2017, 3047
BspT107I GGYRCC 2 cut(s) 1438, 3585
BspTNI GGTCTC 2 cut(s) 3309, 3515
BsrBI CCGCTC 1 cut(s) 2249
BsrDI GCAATG 4 cut(s) 477, 651, 2372, 3096
BsrFI RCCGGY 1 cut(s) 1441
BsrGI TGTACA 1 cut(s) 994
BsrI ACTGG 3 cut(s) 2106, 2926, 3458
BssAI RCCGGY 1 cut(s) 1441
BssECI CCNNGG 7 cut(s) 469, 939, 971, 1631, 2957, 3081, 3438
BssNI GRCGYC 3 cut(s) 967, 3586, 3866
BssSI CACGAG 1 cut(s) 3518
BssT1I CCWWGG 6 cut(s) 469, 939, 971, 2957, 3081, 3438
Bst2BI CACGAG 1 cut(s) 3518
Bst2UI CCWGG 6 cut(s) 607, 1263, 1314, 1632, 1742, 3376
Bst4CI ACNGT 6 cut(s) 239, 921, 1516, 2699, 3041, 3526
Bst6I CTCTTC 7 cut(s) 144, 150, 1168, 1248, 2193, 2364, 2472
BstACI GRCGYC 3 cut(s) 967, 3586, 3866
BstAUI TGTACA 1 cut(s) 994
BstBAI YACGTR 1 cut(s) 3727
BstBI TTCGAA 2 cut(s) 2017, 3047
BstC8I GCNNGC 8 cut(s) 642, 1321, 1325, 1443, 2355, 3570, 3741, 3786
BstDSI CCRYGG 5 cut(s) 939, 971, 2957, 3081, 3438
BstFNI CGCG 2 cut(s) 716, 1436
BstH2I RGCGCY 1 cut(s) 3589
BstHHI GCGC 2 cut(s) 1436, 3588
BstMAI GTCTC 4 cut(s) 1831, 3309, 3515, 3760
BstNI CCWGG 6 cut(s) 607, 1263, 1314, 1632, 1742, 3376
BstNSI RCATGY 3 cut(s) 1555, 2465, 3572
BstSCI CCNGG 8 cut(s) 605, 1261, 1312, 1413, 1630, 1740, 3374, 3844
BstSFI CTRYAG 1 cut(s) 2284
BstSLI GKGCMC 3 cut(s) 477, 3423, 3927
BstUI CGCG 2 cut(s) 716, 1436
BstV1I GCAGC 3 cut(s) 2141, 2469, 3117
BstV2I GAAGAC 2 cut(s) 1554, 1652
BstX2I RGATCY 3 cut(s) 198, 1957, 3371
BstYI RGATCY 3 cut(s) 198, 1957, 3371
BsuI GTATCC 2 cut(s) 1601, 2738
BtgI CCRYGG 5 cut(s) 939, 971, 2957, 3081, 3438
BtrI CACGTC 1 cut(s) 380
BtsIMutI CAGTG 5 cut(s) 244, 926, 1938, 2113, 3481
Cac8I GCNNGC 8 cut(s) 642, 1321, 1325, 1443, 2355, 3570, 3741, 3786
CciI TCATGA 4 cut(s) 1734, 2008, 2413, 2574
CfoI GCGC 2 cut(s) 1436, 3588
Cfr10I RCCGGY 1 cut(s) 1441
Cfr13I GGNCC 4 cut(s) 583, 1055, 2669, 3686
CseI GACGC 4 cut(s) 956, 1145, 2234, 3855
CsiI ACCWGGT 1 cut(s) 1740
CspCI CAANNNNNGTGG 4 cut(s) 246, 281, 3009, 3044
DinI GGCGCC 1 cut(s) 3587
DraI TTTAAA 4 cut(s) 1657, 2100, 2710, 3555
DraIII CACNNNGTG 4 cut(s) 341, 708, 1119, 3064
EaeI YGGCCR 2 cut(s) 2840, 3259
Eam1104I CTCTTC 7 cut(s) 144, 150, 1168, 1248, 2193, 2364, 2472
EarI CTCTTC 7 cut(s) 144, 150, 1168, 1248, 2193, 2364, 2472
EciI GGCGGA 1 cut(s) 3547
Ecl136II GAGCTC 1 cut(s) 2614
Eco130I CCWWGG 6 cut(s) 469, 939, 971, 2957, 3081, 3438
Eco147I AGGCCT 3 cut(s) 468, 817, 1630
Eco24I GRGCYC 3 cut(s) 1430, 2616, 2928
Eco31I GGTCTC 2 cut(s) 3309, 3515
Eco32I GATATC 1 cut(s) 176
Eco47I GGWCC 4 cut(s) 583, 1055, 2669, 3686
Eco53kI GAGCTC 1 cut(s) 2614
Eco57I CTGAAG 4 cut(s) 1227, 3141, 3288, 3722
Eco88I CYCGRG 2 cut(s) 1662, 3823
EcoICRI GAGCTC 1 cut(s) 2614
EcoRI GAATTC 2 cut(s) 2336, 3596
EcoRII CCWGG 6 cut(s) 605, 1261, 1312, 1630, 1740, 3374
EcoRV GATATC 1 cut(s) 176
EcoT14I CCWWGG 6 cut(s) 469, 939, 971, 2957, 3081, 3438
EcoT38I GRGCYC 3 cut(s) 1430, 2616, 2928
EgeI GGCGCC 1 cut(s) 3587
EheI GGCGCC 1 cut(s) 3587
ErhI CCWWGG 6 cut(s) 469, 939, 971, 2957, 3081, 3438
FalI AAGNNNNNCTT 2 cut(s) 316, 348
FaqI GGGAC 7 cut(s) 477, 596, 635, 1068, 1298, 2682, 2816
FauI CCCGC 2 cut(s) 288, 633
FauNDI CATATG 1 cut(s) 1824
FbaI TGATCA 2 cut(s) 2410, 3574
FblI GTMKAC 1 cut(s) 3869
Fnu4HI GCNGC 4 cut(s) 2130, 2483, 2555, 3131
FriOI GRGCYC 3 cut(s) 1430, 2616, 2928
Fsp4HI GCNGC 4 cut(s) 2130, 2483, 2555, 3131
FspBI CTAG 7 cut(s) 420, 470, 629, 1062, 1116, 1716, 2897
GlaI GCGC 2 cut(s) 1435, 3587
GluI GCNGC 4 cut(s) 2130, 2483, 2555, 3131
GsuI CTGGAG 3 cut(s) 250, 628, 1284
HaeII RGCGCY 1 cut(s) 3589
HapII CCGG 5 cut(s) 685, 1414, 1442, 3711, 3846
HgaI GACGC 4 cut(s) 956, 1145, 2234, 3855
HhaI GCGC 2 cut(s) 1436, 3588
Hin1I GRCGYC 3 cut(s) 967, 3586, 3866
Hin6I GCGC 2 cut(s) 1434, 3586
HinP1I GCGC 2 cut(s) 1434, 3586
HincII GTYRAC 4 cut(s) 868, 2441, 3037, 3466
HindII GTYRAC 4 cut(s) 868, 2441, 3037, 3466
HindIII AAGCTT 5 cut(s) 1321, 1868, 2020, 3209, 3512
HpaII CCGG 5 cut(s) 685, 1414, 1442, 3711, 3846
HphI GGTGA 4 cut(s) 327, 1193, 1699, 3620
HpyCH4III ACNGT 6 cut(s) 239, 921, 1516, 2699, 3041, 3526
HpyCH4IV ACGT 3 cut(s) 286, 379, 3726
HpySE526I ACGT 3 cut(s) 286, 379, 3726
Hsp92I GRCGYC 3 cut(s) 967, 3586, 3866
HspAI GCGC 2 cut(s) 1434, 3586
KasI GGCGCC 1 cut(s) 3585
KroI GCCGGC 1 cut(s) 1441
KroNI GCCGGC 1 cut(s) 1443
Ksp22I TGATCA 2 cut(s) 2410, 3574
LguI GCTCTTC 4 cut(s) 150, 2193, 2364, 2472
Lsp1109I GCAGC 3 cut(s) 2141, 2469, 3117
MabI ACCWGGT 1 cut(s) 1740
MaeI CTAG 7 cut(s) 420, 470, 629, 1062, 1116, 1716, 2897
MaeII ACGT 3 cut(s) 286, 379, 3726
MbiI CCGCTC 1 cut(s) 2249
MfeI CAATTG 3 cut(s) 251, 2274, 2653
MflI RGATCY 3 cut(s) 198, 1957, 3371
MlsI TGGCCA 2 cut(s) 2842, 3261
MluNI TGGCCA 2 cut(s) 2842, 3261
Mly113I GGCGCC 1 cut(s) 3586
MlyI GAGTC 8 cut(s) 617, 908, 1360, 3190, 3406, 3815, 3841, 3860
MmeI TCCRAC 4 cut(s) 531, 1315, 1377, 2224
Mox20I TGGCCA 2 cut(s) 2842, 3261
MroNI GCCGGC 1 cut(s) 1441
MroXI GAANNNNTTC 3 cut(s) 331, 452, 2397
MscI TGGCCA 2 cut(s) 2842, 3261
MslI CAYNNNNRTG 4 cut(s) 922, 1724, 2775, 3392
Msp20I TGGCCA 2 cut(s) 2842, 3261
MspA1I CMGCKG 3 cut(s) 2132, 2605, 2878
MspI CCGG 5 cut(s) 685, 1414, 1442, 3711, 3846
MspR9I CCNGG 8 cut(s) 607, 1263, 1314, 1415, 1632, 1742, 3376, 3846
MunI CAATTG 3 cut(s) 251, 2274, 2653
Mva1269I GAATGC 1 cut(s) 1714
MvaI CCWGG 6 cut(s) 607, 1263, 1314, 1632, 1742, 3376
MvnI CGCG 2 cut(s) 716, 1436
NaeI GCCGGC 1 cut(s) 1443
NarI GGCGCC 1 cut(s) 3586
NciI CCSGG 2 cut(s) 1415, 3846
NcoI CCATGG 5 cut(s) 939, 971, 2957, 3081, 3438
NdeI CATATG 1 cut(s) 1824
NgoMIV GCCGGC 1 cut(s) 1441
NmuCI GTSAC 6 cut(s) 1384, 2222, 3092, 3182, 3608, 3814
NspI RCATGY 3 cut(s) 1555, 2465, 3572
NspV TTCGAA 2 cut(s) 2017, 3047
OliI CACNNNNGTG 1 cut(s) 922
PaeI GCATGC 1 cut(s) 3572
PaeR7I CTCGAG 1 cut(s) 3823
PagI TCATGA 4 cut(s) 1734, 2008, 2413, 2574
PceI AGGCCT 3 cut(s) 468, 817, 1630
PciI ACATGT 1 cut(s) 1551
PciSI GCTCTTC 4 cut(s) 150, 2193, 2364, 2472
PctI GAATGC 1 cut(s) 1714
PdiI GCCGGC 1 cut(s) 1443
PdmI GAANNNNTTC 3 cut(s) 331, 452, 2397
PfeI GAWTC 8 cut(s) 85, 368, 671, 1527, 2052, 2521, 3017, 3138
Pfl23II CGTACG 2 cut(s) 3526, 3723
PfoI TCCNGGA 1 cut(s) 1413
PkrI GCNGC 4 cut(s) 2131, 2484, 2556, 3132
PleI GAGTC 8 cut(s) 617, 908, 1359, 3189, 3405, 3815, 3840, 3859
PluTI GGCGCC 1 cut(s) 3589
PpsI GAGTC 8 cut(s) 617, 908, 1359, 3189, 3405, 3815, 3840, 3859
Ppu21I YACGTR 1 cut(s) 3727
PscI ACATGT 1 cut(s) 1551
PshBI ATTAAT 1 cut(s) 1913
PsiI TTATAA 2 cut(s) 2388, 3366
Psp124BI GAGCTC 1 cut(s) 2616
Psp6I CCWGG 6 cut(s) 605, 1261, 1312, 1630, 1740, 3374
PspGI CCWGG 6 cut(s) 605, 1261, 1312, 1630, 1740, 3374
PspLI CGTACG 2 cut(s) 3526, 3723
PspPI GGNCC 4 cut(s) 583, 1055, 2669, 3686
PspXI VCTCGAGB 1 cut(s) 3823
PsuI RGATCY 3 cut(s) 198, 1957, 3371
PvuII CAGCTG 2 cut(s) 2132, 2605
RseI CAYNNNNRTG 4 cut(s) 922, 1724, 2775, 3392
SacI GAGCTC 1 cut(s) 2616
SapI GCTCTTC 4 cut(s) 150, 2193, 2364, 2472
SatI GCNGC 4 cut(s) 2130, 2483, 2555, 3131
Sau96I GGNCC 4 cut(s) 583, 1055, 2669, 3686
SchI GAGTC 8 cut(s) 617, 908, 1360, 3190, 3406, 3815, 3841, 3860
ScrFI CCNGG 8 cut(s) 607, 1263, 1314, 1415, 1632, 1742, 3376, 3846
SexAI ACCWGGT 1 cut(s) 1740
SfcI CTRYAG 1 cut(s) 2284
SfoI GGCGCC 1 cut(s) 3587
Sfr274I CTCGAG 1 cut(s) 3823
SfuI TTCGAA 2 cut(s) 2017, 3047
SinI GGWCC 4 cut(s) 583, 1055, 2669, 3686
SlaI CTCGAG 1 cut(s) 3823
SmiMI CAYNNNNRTG 4 cut(s) 922, 1724, 2775, 3392
SmlI CTYRAG 5 cut(s) 1356, 1834, 2423, 3348, 3823
SmoI CTYRAG 5 cut(s) 1356, 1834, 2423, 3348, 3823
SpeI ACTAGT 1 cut(s) 628
SphI GCATGC 1 cut(s) 3572
SseBI AGGCCT 3 cut(s) 468, 817, 1630
SspDI GGCGCC 1 cut(s) 3585
SspI AATATT 2 cut(s) 766, 3024
SspMI CTAG 7 cut(s) 420, 470, 629, 1062, 1116, 1716, 2897
SstI GAGCTC 1 cut(s) 2616
StuI AGGCCT 3 cut(s) 468, 817, 1630
StyD4I CCNGG 8 cut(s) 605, 1261, 1312, 1413, 1630, 1740, 3374, 3844
StyI CCWWGG 6 cut(s) 469, 939, 971, 2957, 3081, 3438
TaaI ACNGT 6 cut(s) 239, 921, 1516, 2699, 3041, 3526
TaiI ACGT 3 cut(s) 289, 382, 3729
TaqII GACCGA 1 cut(s) 78
TatI WGTACW 3 cut(s) 994, 1948, 3558
TauI GCSGC 1 cut(s) 2557
TfiI GAWTC 8 cut(s) 85, 368, 671, 1527, 2052, 2521, 3017, 3138
TscAI CASTG 5 cut(s) 244, 926, 1945, 2113, 3481
TseFI GTSAC 6 cut(s) 1384, 2222, 3092, 3182, 3608, 3814
TseI GCWGC 3 cut(s) 2129, 2482, 3130
Tsp45I GTSAC 6 cut(s) 1384, 2222, 3092, 3182, 3608, 3814
TspRI CASTG 5 cut(s) 244, 926, 1945, 2113, 3481
VpaK11BI GGWCC 4 cut(s) 583, 1055, 2669, 3686
VspI ATTAAT 1 cut(s) 1913
XapI RAATTY 8 cut(s) 1124, 1232, 1879, 2062, 2336, 2393, 3596, 3625
XceI RCATGY 3 cut(s) 1555, 2465, 3572
XhoI CTCGAG 1 cut(s) 3823
XmaJI CCTAGG 1 cut(s) 469
XmiI GTMKAC 1 cut(s) 3869
XmnI GAANNNNTTC 3 cut(s) 331, 452, 2397
XspI CTAG 7 cut(s) 420, 470, 629, 1062, 1116, 1716, 2897
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.