FvH4_4g33448

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000291
Physical Location & Seq
Reverse (-)
31675331 .. 31677174
1844 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g33448.t1

Sequence Viewer

Length: 1329 bp
ATGAAGAACCCTAAACAAAACCCAAAAACCAATCCTTATGCAAAACCTACTGGAGATATATGTTATCGTTGCAAGAAACTTGGGCATCGTTCCAATGTTTGCCCCGAACGAAAGAAGTTGAATATAGTGGAGAACGAAGGGGAAGTCAATGAAGATGAAGAAGATAGAGAAGATGATGGCTATAGTGGGGCTGAGTTTGCAATTGAAGAGGTTTGGAACGGGGTGCCTATTTCCATTGGGAGACACTATCGTGATGAGGTGACTTGTGATGTTGTTGATATGGATGTCTGTCACATATTGTTGGGGAGGCCTTGGCAGTATGATCTGGATGTCACTCATAAGGGCAGGGATAATGTGATGTTGTTTACATGGAAGGAGCACAAGATTGCTATGGCTCCGTTAATCAATTCTGAAGGCCCCAAGAAAGGAAATTCGAGTTTTCTCACCATTAGCAACAACGATTGTGAGTTGAATGAGGCCATAAAAGAATCCAAAACCATTTTTCCTATAGTGGTTAAGGGATTGATGGCAGTAGATCAAGAAGCAAGGGGAGAGATTCCGAAGGAGGTTTGGGAACTGTTAAAAGGATTTAAGAAGTTAATTGTTGATGATACCCTTACTGAGTTGCCACCCATAAGAGACATTCAACATCAAATAGACTTCATTCTAGGAGCTAGCCTACCTAACCTGCCACATTACCGGATGAGCCCTAAGGAGTGTGAGATCTTAAGGGAGATTATTGAAGATCTGCCAAAGAAGGGGTTTATTCGAGAGAGTATGAGCCCTTGTGCGGTTCCTGTTTTGCTAGTACCTAAGAAAGGAGGACCATGGAGGATGTGTGTGGACAATAGAGTCATCAACAAGATAACAATCAAGTACGGGTTTCCTATCCCACGCCTCGAAGACATGCTTGATGAGTTGAATGGTTCCAAGTGGTTCTCGAAGATAGATTTGAGAAGTGGATATCACCAGATTCGAGTCAAACTTGGAGATGAGTGGAAAACGGCGTTCAAGACCAAAGATGGGTTATATGAGTGGTTGGTTGATGAAGAGAAGGTACGTGCAATTAGAGATTTCAGTACCATCACTACACCAATTACTGAATGCATGAAGAAAGGCAAGTTTCATTGGGGTGAAGAGCAAGAGCAGAGTTTTGCACTTATCAAAGAGAAGTTTTGTACCGCACCGGTTTTGGCTCTTCCAGACTTTGACAAGATATTCAAGGTCGAATGTGATGCTAGTGGTGTTGGAGTGGGGGTTGTCTTGTCCCAAGAAAAGAGGGCAGTGGCTTTCTTTAGCAAGAAGTTGAGTGAAGCTCGCCAAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 Retrotrans_gag rve zf-CCHC zf-H2C2

Protein Analysis

443

Amino Acids

50.56

Weight (kDa)

6.22

Isoelectric Point (pI)

40.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 270 - 342 9.9e-14 Reverse transcriptase (RNA-dependent DNA polymerase)
RT_RNaseH_2 PF17919 377 - 441 1.1e-18 RNase H-like domain found in reverse transcriptase
RT_RNaseH PF17917 402 - 441 8.9e-08 RNase H-like domain found in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000291)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG00860
fragaria_vesca FvH4_1g23712 FvH4_1g29809 FvH4_1g29810 FvH4_2g02661 FvH4_2g13451 FvH4_2g17921 FvH4_2g17922 FvH4_2g19251 FvH4_2g19252 FvH4_2g28221 FvH4_2g35961 FvH4_3g10372 FvH4_3g12461 FvH4_3g12461 FvH4_3g27861 FvH4_3g34591 FvH4_3g40201 FvH4_3g44612 FvH4_4g08352 FvH4_4g12251 FvH4_4g12742 FvH4_4g13904 FvH4_4g14287 FvH4_4g15243 FvH4_4g24071 FvH4_4g33448 FvH4_5g21912 FvH4_5g39371 FvH4_6g14992 FvH4_6g15221 FvH4_6g20603 FvH4_6g31093 FvH4_6g32372 FvH4_6g33012
malus_domestica MD11G1009100.v1.1 MD13G1145000.v1.1 MD16G1138700.v1.1
prunus_persica Prupe.5G246300_v2.0.a1 Prupe.7G034800_v2.0.a1 Prupe.8G084500_v2.0.a1
pyrus_communis pycom01g18240 pycom04g00330 pycom05g09010 pycom06g01670 pycom06g01690 pycom06g07180 pycom06g11620 pycom10g05720 pycom1134g00040 pycom1134g00060 pycom12486g00010 pycom12555g00230 pycom13g21670 pycom13g23130 pycom13g23140 pycom14g10580
rosa_chinensis RchiOBHm_Chr1g0330691 RchiOBHm_Chr2g0106171 RchiOBHm_Chr2g0134731 RchiOBHm_Chr2g0142791 RchiOBHm_Chr2g0149861 RchiOBHm_Chr3g0452401 RchiOBHm_Chr3g0461881 RchiOBHm_Chr4g0401341 RchiOBHm_Chr5g0075641 RchiOBHm_Chr6g0265961 RchiOBHm_Chr7g0200201 RchiOBHm_Chr7g0237811
rosa_laevigata RLG00000002773
rosa_multiflora Rmu_co8037806.1_g000001 Rmu_co8445989.1_g000001 Rmu_sc0000745.1_g000039 Rmu_sc0004448.1_g000029 Rmu_sc0004488.1_g000002 Rmu_sc0004591.1_g000018 Rmu_sc0005065.1_g000030 Rmu_sc0005150.1_g000005 Rmu_sc0005654.1_g000011 Rmu_sc0006986.1_g000016
rosa_roxburghii Rroxscaffold_164G00436490 Rroxscaffold_175G00432490 Rroxscaffold_1G00059440 Rroxscaffold_1G00064410 Rroxscaffold_2G00100310 Rroxscaffold_2G00128170 Rroxscaffold_3G00239420 Rroxscaffold_3G00264970 Rroxscaffold_3G00271550 Rroxscaffold_4G00300240 Rroxscaffold_5G00385050 Rroxscaffold_6G00399720
rosa_rugosa Rorug02G0252200
rosa_samantha Rh2DG251900 Rh7CG329900
rosa_wichuraiana Rw0G004800 Rw1G000850 Rw1G022350 Rw2G027030 Rw2G027760 Rw2G028410 Rw2G029130 Rw2G029390 Rw2G032490 Rw2G039460 Rw2G047800 Rw2G050340 Rw3G026430 Rw4G018370 Rw4G030750 Rw4G033140 Rw5G011950 Rw5G012010 Rw5G029280 Rw5G040190 Rw5G044570 Rw5G048240 Rw6G000340 Rw6G010140 Rw6G026480 Rw7G033750 Rw7G037250 Rw7G042080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 851
Acc36I ACCTGC 1 cut(s) 696
AccB1I GGYRCC 1 cut(s) 223
AciI CCGC 2 cut(s) 791, 1182
AcsI RAATTY 1 cut(s) 430
AcuI CTGAAG 1 cut(s) 432
AfaI GTAC 5 cut(s) 810, 878, 1059, 1081, 1180
AfiI CCNNNNNNNGG 5 cut(s) 425, 758, 790, 818, 1023
AflII CTTAAG 1 cut(s) 727
AgeI ACCGGT 1 cut(s) 1186
AgsI TTSAA 8 cut(s) 121, 206, 472, 647, 743, 922, 1012, 1222
AleI CACNNNNGTG 1 cut(s) 249
AluBI AGCT 2 cut(s) 674, 1316
AluI AGCT 2 cut(s) 674, 1316
Alw21I GWGCWC 1 cut(s) 381
Alw26I GTCTC 2 cut(s) 235, 633
AoxI GGCC 3 cut(s) 308, 415, 477
ApoI RAATTY 1 cut(s) 430
ArsI GACNNNNNNTTYG 2 cut(s) 129, 161
AsiGI ACCGGT 1 cut(s) 1186
AspS9I GGNCC 2 cut(s) 416, 824
AsuHPI GGTGA 4 cut(s) 271, 436, 959, 1145
AsuNHI GCTAGC 1 cut(s) 674
AvaII GGWCC 1 cut(s) 824
AxyI CCTNAGG 1 cut(s) 711
BanI GGYRCC 1 cut(s) 223
BanII GRGCYC 2 cut(s) 710, 785
BarI GAAGNNNNNNTAC 2 cut(s) 1041, 1073
BbsI GAAGAC 1 cut(s) 909
Bbv12I GWGCWC 1 cut(s) 381
BccI CCATC 4 cut(s) 170, 520, 1016, 1091
BceAI ACGGC 1 cut(s) 1020
BcgI CGANNNNNNTGC 2 cut(s) 1217, 1251
BcoDI GTCTC 2 cut(s) 235, 633
BfaI CTAG 4 cut(s) 668, 675, 806, 1239
BfmI CTRYAG 2 cut(s) 181, 507
BfrI CTTAAG 1 cut(s) 727
BfuAI ACCTGC 1 cut(s) 696
BglII AGATCT 2 cut(s) 723, 745
Bme18I GGWCC 1 cut(s) 824
BmgT120I GGNCC 2 cut(s) 416, 824
BmiI GGNNCC 5 cut(s) 225, 396, 418, 795, 928
BmsI GCATC 2 cut(s) 94, 1225
BmtI GCTAGC 1 cut(s) 678
BpiI GAAGAC 1 cut(s) 909
BplI GAGNNNNNCTC 1 cut(s) 1300
BpmI CTGGAG 1 cut(s) 72
BsaAI YACGTR 1 cut(s) 1061
BsaBI GATNNNNATC 1 cut(s) 869
BsaJI CCNNGG 2 cut(s) 311, 827
BsaWI WCCGGW 2 cut(s) 699, 1186
BsaXI ACNNNNNCTCC 4 cut(s) 823, 853, 1242, 1272
Bsc4I CCNNNNNNNGG 5 cut(s) 425, 758, 790, 818, 1023
Bse118I RCCGGY 1 cut(s) 1186
Bse1I ACTGG 1 cut(s) 55
Bse21I CCTNAGG 1 cut(s) 711
Bse8I GATNNNNATC 1 cut(s) 869
BseDI CCNNGG 2 cut(s) 311, 827
BseGI GGATG 4 cut(s) 289, 334, 708, 840
BseJI GATNNNNATC 1 cut(s) 869
BseLI CCNNNNNNNGG 5 cut(s) 425, 758, 790, 818, 1023
BseMII CTCAG 2 cut(s) 183, 612
BseNI ACTGG 1 cut(s) 55
BshFI GGCC 3 cut(s) 310, 417, 479
BshNI GGYRCC 1 cut(s) 223
BshTI ACCGGT 1 cut(s) 1186
BsiHKAI GWGCWC 1 cut(s) 381
BsiSI CCGG 2 cut(s) 700, 1187
BslFI GGGAC 1 cut(s) 1252
BslI CCNNNNNNNGG 5 cut(s) 425, 758, 790, 818, 1023
BsmAI GTCTC 2 cut(s) 235, 633
BsmFI GGGAC 1 cut(s) 1252
BsmI GAATGC 1 cut(s) 1109
BsnI GGCC 3 cut(s) 310, 417, 479
Bsp1286I GDGCHC 3 cut(s) 381, 710, 785
Bsp143I GATC 4 cut(s) 322, 535, 723, 745
Bsp19I CCATGG 1 cut(s) 827
BspACI CCGC 2 cut(s) 791, 1182
BspANI GGCC 3 cut(s) 310, 417, 479
BspCNI CTCAG 2 cut(s) 184, 613
BspLI GGNNCC 5 cut(s) 225, 396, 418, 795, 928
BspMI ACCTGC 1 cut(s) 696
BspOI GCTAGC 1 cut(s) 678
BspQI GCTCTTC 2 cut(s) 1131, 1203
BspT107I GGYRCC 1 cut(s) 223
BspTI CTTAAG 1 cut(s) 727
BsrFI RCCGGY 1 cut(s) 1186
BsrI ACTGG 1 cut(s) 55
BssAI RCCGGY 1 cut(s) 1186
BssECI CCNNGG 2 cut(s) 311, 827
BssMI GATC 4 cut(s) 322, 535, 723, 745
BssT1I CCWWGG 2 cut(s) 311, 827
Bst4CI ACNGT 1 cut(s) 579
Bst6I CTCTTC 4 cut(s) 201, 1044, 1131, 1203
BstAFI CTTAAG 1 cut(s) 727
BstBAI YACGTR 1 cut(s) 1061
BstC8I GCNNGC 2 cut(s) 676, 1318
BstDEI CTNAG 4 cut(s) 192, 621, 711, 813
BstDSI CCRYGG 1 cut(s) 827
BstENI CCTNNNNNAGG 1 cut(s) 816
BstF5I GGATG 4 cut(s) 289, 334, 708, 840
BstKTI GATC 4 cut(s) 325, 538, 726, 748
BstMAI GTCTC 2 cut(s) 235, 633
BstMBI GATC 4 cut(s) 322, 535, 723, 745
BstMWI GCNNNNNNNGC 1 cut(s) 197
BstNSI RCATGY 1 cut(s) 910
BstSFI CTRYAG 2 cut(s) 181, 507
BstV2I GAAGAC 1 cut(s) 909
BstX2I RGATCY 2 cut(s) 723, 745
BstYI RGATCY 2 cut(s) 723, 745
Bsu36I CCTNAGG 1 cut(s) 711
BsuRI GGCC 3 cut(s) 310, 417, 479
BtgI CCRYGG 1 cut(s) 827
BtsCI GGATG 4 cut(s) 289, 334, 708, 840
BtsI GCAGTG 1 cut(s) 1290
BtsIMutI CAGTG 1 cut(s) 1290
BveI ACCTGC 1 cut(s) 696
Cac8I GCNNGC 2 cut(s) 676, 1318
Cfr10I RCCGGY 1 cut(s) 1186
Cfr13I GGNCC 2 cut(s) 416, 824
Csp6I GTAC 5 cut(s) 809, 877, 1058, 1080, 1179
CspAI ACCGGT 1 cut(s) 1186
CviAII CATG 4 cut(s) 369, 828, 907, 1108
CviQI GTAC 5 cut(s) 809, 877, 1058, 1080, 1179
DdeI CTNAG 4 cut(s) 192, 621, 711, 813
DpnI GATC 4 cut(s) 324, 537, 725, 747
DpnII GATC 4 cut(s) 322, 535, 723, 745
DrdI GACNNNNNNGTC 1 cut(s) 851
DseDI GACNNNNNNGTC 1 cut(s) 851
Eam1104I CTCTTC 4 cut(s) 201, 1044, 1131, 1203
EarI CTCTTC 4 cut(s) 201, 1044, 1131, 1203
Eco130I CCWWGG 2 cut(s) 311, 827
Eco147I AGGCCT 1 cut(s) 310
Eco24I GRGCYC 2 cut(s) 710, 785
Eco32I GATATC 1 cut(s) 965
Eco47I GGWCC 1 cut(s) 824
Eco57I CTGAAG 1 cut(s) 432
Eco81I CCTNAGG 1 cut(s) 711
EcoNI CCTNNNNNAGG 1 cut(s) 816
EcoO109I RGGNCCY 1 cut(s) 416
EcoRV GATATC 1 cut(s) 965
EcoT14I CCWWGG 2 cut(s) 311, 827
EcoT22I ATGCAT 1 cut(s) 1109
EcoT38I GRGCYC 2 cut(s) 710, 785
ErhI CCWWGG 2 cut(s) 311, 827
FaeI CATG 4 cut(s) 372, 831, 910, 1111
FalI AAGNNNNNCTT 2 cut(s) 894, 926
FaqI GGGAC 1 cut(s) 1252
FatI CATG 4 cut(s) 368, 827, 906, 1107
FokI GGATG 4 cut(s) 296, 341, 715, 847
FriOI GRGCYC 2 cut(s) 710, 785
FspBI CTAG 4 cut(s) 668, 675, 806, 1239
GsuI CTGGAG 1 cut(s) 72
HaeIII GGCC 3 cut(s) 310, 417, 479
HapII CCGG 2 cut(s) 700, 1187
Hin1II CATG 4 cut(s) 372, 831, 910, 1111
HinfI GANTC 5 cut(s) 488, 556, 852, 973, 978
HpaII CCGG 2 cut(s) 700, 1187
HphI GGTGA 4 cut(s) 271, 436, 959, 1145
Hpy166II GTNNAC 2 cut(s) 366, 844
Hpy188I TCNGA 2 cut(s) 412, 561
Hpy188III TCNNGA 7 cut(s) 251, 326, 539, 770, 940, 1012, 1202
Hpy8I GTNNAC 2 cut(s) 366, 844
HpyAV CCTTC 6 cut(s) 131, 367, 407, 556, 751, 1048
HpyCH4III ACNGT 1 cut(s) 579
HpyCH4IV ACGT 1 cut(s) 1060
HpyCH4V TGCA 6 cut(s) 41, 72, 200, 1064, 1107, 1157
HpyF10VI GCNNNNNNNGC 1 cut(s) 197
HpyF3I CTNAG 4 cut(s) 192, 621, 711, 813
HpySE526I ACGT 1 cut(s) 1060
Hsp92II CATG 4 cut(s) 372, 831, 910, 1111
Kzo9I GATC 4 cut(s) 322, 535, 723, 745
LguI GCTCTTC 2 cut(s) 1131, 1203
LmnI GCTCC 3 cut(s) 376, 400, 671
LpnPI CCDG 9 cut(s) 36, 311, 331, 701, 713, 810, 983, 1200, 1215
LweI GCATC 2 cut(s) 94, 1225
MaeI CTAG 4 cut(s) 668, 675, 806, 1239
MaeII ACGT 1 cut(s) 1060
MaeIII GTNAC 3 cut(s) 259, 290, 331
MalI GATC 4 cut(s) 324, 537, 725, 747
MboI GATC 4 cut(s) 322, 535, 723, 745
MfeI CAATTG 1 cut(s) 201
MflI RGATCY 2 cut(s) 723, 745
MhlI GDGCHC 3 cut(s) 381, 710, 785
MluCI AATT 6 cut(s) 201, 406, 430, 600, 1065, 1095
MlyI GAGTC 2 cut(s) 861, 987
MmeI TCCRAC 1 cut(s) 1228
MnlI CCTC 9 cut(s) 202, 250, 300, 469, 559, 815, 825, 908, 1272
Mph1103I ATGCAT 1 cut(s) 1109
MseI TTAA 6 cut(s) 401, 516, 581, 591, 599, 728
MslI CAYNNNNRTG 2 cut(s) 249, 1131
MspCI CTTAAG 1 cut(s) 727
MspI CCGG 2 cut(s) 700, 1187
MunI CAATTG 1 cut(s) 201
Mva1269I GAATGC 1 cut(s) 1109
MwoI GCNNNNNNNGC 1 cut(s) 197
NcoI CCATGG 1 cut(s) 827
NdeII GATC 4 cut(s) 322, 535, 723, 745
NheI GCTAGC 1 cut(s) 674
NlaIII CATG 4 cut(s) 372, 831, 910, 1111
NlaIV GGNNCC 5 cut(s) 225, 396, 418, 795, 928
NmuCI GTSAC 3 cut(s) 259, 290, 331
NsiI ATGCAT 1 cut(s) 1109
NspI RCATGY 1 cut(s) 910
OliI CACNNNNGTG 1 cut(s) 249
PceI AGGCCT 1 cut(s) 310
PciSI GCTCTTC 2 cut(s) 1131, 1203
PctI GAATGC 1 cut(s) 1109
PfeI GAWTC 3 cut(s) 488, 556, 973
PinAI ACCGGT 1 cut(s) 1186
PleI GAGTC 2 cut(s) 860, 986
PpsI GAGTC 2 cut(s) 860, 986
Ppu21I YACGTR 1 cut(s) 1061
PspN4I GGNNCC 5 cut(s) 225, 396, 418, 795, 928
PspPI GGNCC 2 cut(s) 416, 824
PsuI RGATCY 2 cut(s) 723, 745
RsaI GTAC 5 cut(s) 810, 878, 1059, 1081, 1180
RsaNI GTAC 5 cut(s) 809, 877, 1058, 1080, 1179
RseI CAYNNNNRTG 2 cut(s) 249, 1131
SapI GCTCTTC 2 cut(s) 1131, 1203
SaqAI TTAA 6 cut(s) 401, 516, 581, 591, 599, 728
Sau3AI GATC 4 cut(s) 322, 535, 723, 745
Sau96I GGNCC 2 cut(s) 416, 824
SchI GAGTC 2 cut(s) 861, 987
SduI GDGCHC 3 cut(s) 381, 710, 785
SfaNI GCATC 2 cut(s) 94, 1225
SfcI CTRYAG 2 cut(s) 181, 507
SinI GGWCC 1 cut(s) 824
SmiMI CAYNNNNRTG 2 cut(s) 249, 1131
SmlI CTYRAG 1 cut(s) 727
SmoI CTYRAG 1 cut(s) 727
Sse9I AATT 6 cut(s) 201, 406, 430, 600, 1065, 1095
SseBI AGGCCT 1 cut(s) 310
SsiI CCGC 2 cut(s) 791, 1182
SspMI CTAG 4 cut(s) 668, 675, 806, 1239
StuI AGGCCT 1 cut(s) 310
StyI CCWWGG 2 cut(s) 311, 827
TaaI ACNGT 1 cut(s) 579
TaiI ACGT 1 cut(s) 1063
TaqI TCGA 6 cut(s) 434, 769, 900, 941, 976, 1227
TasI AATT 6 cut(s) 201, 406, 430, 600, 1065, 1095
TfiI GAWTC 3 cut(s) 488, 556, 973
Tru1I TTAA 6 cut(s) 401, 516, 581, 591, 599, 728
Tru9I TTAA 6 cut(s) 401, 516, 581, 591, 599, 728
TscAI CASTG 1 cut(s) 1290
TseFI GTSAC 3 cut(s) 259, 290, 331
Tsp45I GTSAC 3 cut(s) 259, 290, 331
TspDTI ATGAA 7 cut(s) 17, 165, 171, 652, 1062, 1115, 1124
TspGWI ACGGA 1 cut(s) 387
TspRI CASTG 1 cut(s) 1290
Vha464I CTTAAG 1 cut(s) 727
VpaK11BI GGWCC 1 cut(s) 824
XagI CCTNNNNNAGG 1 cut(s) 816
XapI RAATTY 1 cut(s) 430
XceI RCATGY 1 cut(s) 910
XspI CTAG 4 cut(s) 668, 675, 806, 1239
Zsp2I ATGCAT 1 cut(s) 1109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.