FvH4_3g40201

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000291
Physical Location & Seq
Forward (+)
33986362 .. 33989302
2941 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g40201.t1

Sequence Viewer

Length: 2721 bp
ATGGGTGACGAAGCTCCTATTACTCGAGCAGAGTTCAATGCTCAGAATGAACGTCTCAATGCTCAGATGGGAGAGATCCGACAACTGTTGCTTGGCTTGAGCAACAACAACAACAACAACAATGAGAACAACAACCTCAACCGTCGGGATCGTGGAAGGCAACCACAACAACGTGTTGTGGAACGCGTTGTGGAGAATGATGAATCGGATTCAGAGGAAGAGTTGTCAGTTGCAGGTGACGATCAGATTAATCAATCTGATTACAAGATGAAGGCTGAAATTCCAACCTTCTCAGGACATCTGAAAATTGAAGAATTCCTTGATTGGCTGGTAGAAGTTGACAGATTCTTCGACATGATGGAAGTGCAAGAGTCAAAGAGAGTGAAGATGGTCGATTTCAGGCTGAAGAGTAGTGCTGCTGTATGGTGGGATCAATTGCAGCAAACTCGTCGAAGACAGGGAAAGAAGCCGATTCAAACATGGTGGAGGATGCAGCAGATGTTGAGGGACAGATTTCTTCCAGCAGATTACGAACAACTGCTCTATCGCATGTACATGGATTGTGTACAAGGCAATAGAACGGTAGATGAGTACACAAATGAGTTCTTGAGGTTAGCAGAACGCAATCAATTGGGAGAGTCAGAGGCGCAAAAAAGTGGCAAGGCAGAGCTAATGGAGAAAGCAAAAAGGACTTATTCCAATGGTGAGAGAGGAAGGCAGATTCCTATACCTGATAGGGTTTCTACCTCTTTCAAAAGAGCAGAAAACAGCAGCAGTAGCAGCAGCAAGGTGAACCTGGTGGAGACGCAAACTCAAGGAGATGTCAATGAAGATGAGGAAGATAGAGAAGACGATGACTATAGTGGGGTTGAGTTTGCAATTGAAGAGGGTTTGGATAGGGTGACTTTAGTGCTGCAGAGGGTCATGTTGGCGCCAAAAGAAAAAGGACAACGCCACAATATATTCCGTTCGGCATGCTCTATGAATAACAAGGTATGTCAGGTGATTGTGGATAATGGGAGTTGTGAGAACTTTGTGTCTCATAAACTGGTGGAGTTTTTGAAGTTTCCTACAAAACCTCATCCTAAGCCTTATAAACTAGGCTGGGTGAAAAAAGGACCTCAAGCAGAAGTGACCACAACATGCAGGGTGCCTATTTCCATTGGGAGACACTATCGTGACCAGGTGAGTTGTGATGTTGTTGATATGGATGTCTGTCACATATTGTTGGGGAGGCCTTGGCAGTATGATCTGGATGTCACTCATAAGGGCAGGGATAATGTGATGTTGTTTACATGGAAGGAGCACAAGATTGCTATGGCTCCGTTAAGCAATTCTGAAGGTCCCAAGAAAGGAAATTCGAGTTTTCTCACCATCAGCAACAACGATTGTGAGTTGAATGAGGCCATAAAAGAATCCAAAACCATTTTTCCTATAGTGGTTAAGGGTTTGATGACAGTAGAGAAGCAAGGCGGAGAGATTCCGAAGGAGGTCTGGGAGTTGTTAGAAGGATTTAAGGAGTTAATTGCTGATGATATTCCTAATGAGCTGCCACCCATGAGAGACATTCAACATCAAATAGACTTCATTCCAGGAGCTAGTCTACCTAACCTGCCACACTACAGAATGAGCCCTAAAGAATGTGAGATCTTAAGGGAGATTATTGAAGATTTGCTAAAGAAAGGGTTTATTCGAGAGAGTATGAGCCCTTGTGCGGTTCCTGTTTTGTTGGTACCTAAGAAAGGAGGAACATGGAGGATGTGTGTGGACAGCAGAGCCATCAACAAGATAACAATCAAGTACAGGTTTCCTATCCCACGCCTTGAAGACATGCTTGATGAGTTGAATGGTTCCAAGTGGTTCTCGAAGATAGATTTGAGGAGTGGATATCACCAGATTCGAGTCAAACCTGGGGATGAGTGGAAAACTGCGTTCAAGACCAAAGATGGGTTATATGAGTGGTTGGTGATGCCTTTTGGGTTGTCCAACGCTCCTAGCACCTTTATGAGACTTATGAATCAGGTTCTTCGTCCTTTCATTGGAACCAGGGTTCTTGTTTATTTCGATGATATCCTTATTTATAGTAAGCAGAAAACCGAGCACCTAGAACATGTGAGACAAGTTCTTGAGGTGTTACGGGATAATAAGTTGTACATCAACCTGAAGAAGTGTACTTTTTGCACCAATAAGCTGCTGTTTCTAGGCTTTGTGGTTGGAGAGGATGGGATTCAGGTTGATGAAGAGAAGGTACGTGCAATTAGAGAGTGGCCAACTCCTAGAACAGTTTCGGAGGTGAGAAGTTTCCATGGTTTAGCTACTTTCTATAGACGGTTTGTGAGGGATTTCAGTACCATCACTGCACCAATTACTGAATGCATGAAGAAAGGCAAGTTTCATTGGGGTGAAGAGCAAGAGCAAAGTTTTGCACTTATCAAAGAGAAGCTTTGTACCGCACCAGTTTTGGCTCTTCCAGATTTTGACAAGATATTCGAGGTCGAATGTGATGCTAGTGGTGTTGGAGTGGGGGCTATCTTGTCCCAAGAAAAGAGGGCAGTGGCTTTCTTTAGCGAGAAGTTGAGTGAAGCTCGCCAAAAATCGAGTACATATGACCAAGAGTTCTATGCAGTAGTGAGAGCTTTAAGGCAATGGGAGCATTATCTGATCCAAAGAGAGTTTATTCTGTTCACTGATCATCAGGCTTTGAAGTTTCTGAACAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 Retrotrans_gag rve zf-CCHC zf-H2C2

Protein Analysis

907

Amino Acids

105.01

Weight (kDa)

6.28

Isoelectric Point (pI)

44.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotrans_gag PF03732 129 - 217 4.2e-12 Retrotransposon gag protein
RVT_1 PF00078 578 - 737 8.4e-45 Reverse transcriptase (RNA-dependent DNA polymerase)
RT_RNaseH_2 PF17919 800 - 893 2.8e-29 RNase H-like domain found in reverse transcriptase
RT_RNaseH PF17917 825 - 905 5.2e-25 RNase H-like domain found in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000291)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG00860
fragaria_vesca FvH4_1g23712 FvH4_1g29809 FvH4_1g29810 FvH4_2g02661 FvH4_2g13451 FvH4_2g17921 FvH4_2g17922 FvH4_2g19251 FvH4_2g19252 FvH4_2g28221 FvH4_2g35961 FvH4_3g10372 FvH4_3g12461 FvH4_3g12461 FvH4_3g27861 FvH4_3g34591 FvH4_3g40201 FvH4_3g44612 FvH4_4g08352 FvH4_4g12251 FvH4_4g12742 FvH4_4g13904 FvH4_4g14287 FvH4_4g15243 FvH4_4g24071 FvH4_4g33448 FvH4_5g21912 FvH4_5g39371 FvH4_6g14992 FvH4_6g15221 FvH4_6g20603 FvH4_6g31093 FvH4_6g32372 FvH4_6g33012
malus_domestica MD11G1009100.v1.1 MD13G1145000.v1.1 MD16G1138700.v1.1
prunus_persica Prupe.5G246300_v2.0.a1 Prupe.7G034800_v2.0.a1 Prupe.8G084500_v2.0.a1
pyrus_communis pycom01g18240 pycom04g00330 pycom05g09010 pycom06g01670 pycom06g01690 pycom06g07180 pycom06g11620 pycom10g05720 pycom1134g00040 pycom1134g00060 pycom12486g00010 pycom12555g00230 pycom13g21670 pycom13g23130 pycom13g23140 pycom14g10580
rosa_chinensis RchiOBHm_Chr1g0330691 RchiOBHm_Chr2g0106171 RchiOBHm_Chr2g0134731 RchiOBHm_Chr2g0142791 RchiOBHm_Chr2g0149861 RchiOBHm_Chr3g0452401 RchiOBHm_Chr3g0461881 RchiOBHm_Chr4g0401341 RchiOBHm_Chr5g0075641 RchiOBHm_Chr6g0265961 RchiOBHm_Chr7g0200201 RchiOBHm_Chr7g0237811
rosa_laevigata RLG00000002773
rosa_multiflora Rmu_co8037806.1_g000001 Rmu_co8445989.1_g000001 Rmu_sc0000745.1_g000039 Rmu_sc0004448.1_g000029 Rmu_sc0004488.1_g000002 Rmu_sc0004591.1_g000018 Rmu_sc0005065.1_g000030 Rmu_sc0005150.1_g000005 Rmu_sc0005654.1_g000011 Rmu_sc0006986.1_g000016
rosa_roxburghii Rroxscaffold_164G00436490 Rroxscaffold_175G00432490 Rroxscaffold_1G00059440 Rroxscaffold_1G00064410 Rroxscaffold_2G00100310 Rroxscaffold_2G00128170 Rroxscaffold_3G00239420 Rroxscaffold_3G00264970 Rroxscaffold_3G00271550 Rroxscaffold_4G00300240 Rroxscaffold_5G00385050 Rroxscaffold_6G00399720
rosa_rugosa Rorug02G0252200
rosa_samantha Rh2DG251900 Rh7CG329900
rosa_wichuraiana Rw0G004800 Rw1G000850 Rw1G022350 Rw2G027030 Rw2G027760 Rw2G028410 Rw2G029130 Rw2G029390 Rw2G032490 Rw2G039460 Rw2G047800 Rw2G050340 Rw3G026430 Rw4G018370 Rw4G030750 Rw4G033140 Rw5G011950 Rw5G012010 Rw5G029280 Rw5G040190 Rw5G044570 Rw5G048240 Rw6G000340 Rw6G010140 Rw6G026480 Rw7G033750 Rw7G037250 Rw7G042080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1095
AarI CACCTGC 1 cut(s) 224
Acc36I ACCTGC 2 cut(s) 224, 1620
Acc65I GGTACC 1 cut(s) 1732
AccB1I GGYRCC 3 cut(s) 931, 1150, 1732
AccI GTMKAC 1 cut(s) 1603
AccII CGCG 1 cut(s) 186
AciI CCGC 3 cut(s) 1473, 1715, 2451
AclWI GGATC 4 cut(s) 70, 156, 438, 2656
AcoI YGGCCR 1 cut(s) 2267
AcsI RAATTY 3 cut(s) 279, 314, 1357
AcuI CTGAAG 3 cut(s) 425, 1359, 2183
AcyI GRCGYC 1 cut(s) 932
AfiI CCNNNNNNNGG 5 cut(s) 1352, 1714, 1742, 1947, 2039
AflII CTTAAG 1 cut(s) 1651
AflIII ACRYGT 3 cut(s) 172, 184, 2110
AjnI CCWGG 5 cut(s) 795, 1182, 1591, 1909, 2045
AleI CACNNNNGTG 1 cut(s) 1176
AloI GAACNNNNNNTCC 2 cut(s) 2034, 2066
AluBI AGCT 9 cut(s) 14, 670, 1549, 1598, 2191, 2315, 2443, 2585, 2636
AluI AGCT 9 cut(s) 14, 670, 1549, 1598, 2191, 2315, 2443, 2585, 2636
Alw21I GWGCWC 2 cut(s) 1308, 2103
Alw26I GTCTC 7 cut(s) 59, 797, 1044, 1162, 1557, 2002, 2110
AlwI GGATC 4 cut(s) 70, 156, 438, 2656
Ama87I CYCGRG 1 cut(s) 24
AoxI GGCC 3 cut(s) 1235, 1404, 2267
ApeKI GCWGC 9 cut(s) 416, 439, 493, 771, 780, 783, 913, 1549, 2191
ApoI RAATTY 3 cut(s) 279, 314, 1357
ArsI GACNNNNNNTTYG 4 cut(s) 332, 364, 2471, 2503
AseI ATTAAT 1 cut(s) 249
Asp700I GAANNNNTTC 1 cut(s) 2284
Asp718I GGTACC 1 cut(s) 1732
AspLEI GCGC 2 cut(s) 649, 934
AspS9I GGNCC 2 cut(s) 1118, 1343
AvaI CYCGRG 1 cut(s) 24
AvaII GGWCC 2 cut(s) 1118, 1343
BalI TGGCCA 1 cut(s) 2269
BanI GGYRCC 3 cut(s) 931, 1150, 1732
BanII GRGCYC 2 cut(s) 1634, 1709
BarI GAAGNNNNNNTAC 2 cut(s) 2232, 2264
BbsI GAAGAC 3 cut(s) 460, 855, 1833
Bbv12I GWGCWC 2 cut(s) 1308, 2103
BbvI GCAGC 9 cut(s) 403, 451, 505, 783, 792, 795, 900, 1536, 2178
BccI CCATC 8 cut(s) 61, 352, 382, 1382, 1787, 1940, 2216, 2360
BcgI CGANNNNNNTGC 6 cut(s) 428, 431, 462, 465, 2486, 2520
BciT130I CCWGG 5 cut(s) 797, 1184, 1593, 1911, 2047
BclI TGATCA 1 cut(s) 2689
BcoDI GTCTC 7 cut(s) 59, 797, 1044, 1162, 1557, 2002, 2110
BfaI CTAG 7 cut(s) 1100, 1599, 1995, 2105, 2201, 2277, 2508
BfmI CTRYAG 5 cut(s) 859, 914, 1434, 1621, 2323
BfoI RGCGCY 1 cut(s) 935
BfrI CTTAAG 1 cut(s) 1651
BfuAI ACCTGC 2 cut(s) 224, 1620
BglII AGATCT 1 cut(s) 1647
BisI GCNGC 9 cut(s) 417, 440, 494, 772, 781, 784, 914, 1550, 2192
BlsI GCNGC 9 cut(s) 418, 441, 495, 773, 782, 785, 915, 1551, 2193
Bme1390I CCNGG 5 cut(s) 797, 1184, 1593, 1911, 2047
Bme18I GGWCC 2 cut(s) 1118, 1343
BmeT110I CYCGRG 1 cut(s) 24
BmgT120I GGNCC 2 cut(s) 1118, 1343
BmiI GGNNCC 8 cut(s) 933, 1152, 1323, 1345, 1719, 1734, 1852, 2044
BmrFI CCNGG 5 cut(s) 797, 1184, 1593, 1911, 2047
BmsI GCATC 3 cut(s) 480, 1959, 2494
BpiI GAAGAC 3 cut(s) 460, 855, 1833
BplI GAGNNNNNCTC 2 cut(s) 2569, 2601
Bpu10I CCTNAGC 1 cut(s) 1086
BpuEI CTTGAG 5 cut(s) 118, 628, 798, 1107, 2147
BsaAI YACGTR 1 cut(s) 2252
BsaBI GATNNNNATC 1 cut(s) 1793
BsaHI GRCGYC 1 cut(s) 932
BsaJI CCNNGG 4 cut(s) 1238, 1910, 2046, 2305
BsaXI ACNNNNNCTCC 2 cut(s) 1747, 1777
Bsc4I CCNNNNNNNGG 5 cut(s) 1352, 1714, 1742, 1947, 2039
Bse1I ACTGG 2 cut(s) 1053, 2456
Bse3DI GCAATG 1 cut(s) 2651
Bse8I GATNNNNATC 1 cut(s) 1793
BseBI CCWGG 5 cut(s) 797, 1184, 1593, 1911, 2047
BseDI CCNNGG 4 cut(s) 1238, 1910, 2046, 2305
BseGI GGATG 7 cut(s) 495, 1081, 1216, 1261, 1764, 1921, 2227
BseJI GATNNNNATC 1 cut(s) 1793
BseLI CCNNNNNNNGG 5 cut(s) 1352, 1714, 1742, 1947, 2039
BseMI GCAATG 1 cut(s) 2651
BseMII CTCAG 3 cut(s) 56, 77, 306
BseNI ACTGG 2 cut(s) 1053, 2456
BseRI GAGGAG 1 cut(s) 1894
BseXI GCAGC 9 cut(s) 403, 451, 505, 783, 792, 795, 900, 1536, 2178
BseYI CCCAGC 1 cut(s) 1104
BsgI GTGCAG 1 cut(s) 2343
Bsh1236I CGCG 1 cut(s) 186
BshFI GGCC 3 cut(s) 1237, 1406, 2269
BshNI GGYRCC 3 cut(s) 931, 1150, 1732
BsiHKAI GWGCWC 2 cut(s) 1308, 2103
BsiHKCI CYCGRG 1 cut(s) 24
BslFI GGGAC 3 cut(s) 521, 1329, 2521
BslI CCNNNNNNNGG 5 cut(s) 1352, 1714, 1742, 1947, 2039
BsmAI GTCTC 7 cut(s) 59, 797, 1044, 1162, 1557, 2002, 2110
BsmBI CGTCTC 2 cut(s) 59, 797
BsmFI GGGAC 3 cut(s) 521, 1329, 2521
BsmI GAATGC 1 cut(s) 2378
BsnI GGCC 3 cut(s) 1237, 1406, 2269
BsoBI CYCGRG 1 cut(s) 24
Bsp1286I GDGCHC 4 cut(s) 1308, 1634, 1709, 2103
Bsp1407I TGTACA 3 cut(s) 552, 565, 2151
Bsp143I GATC 8 cut(s) 75, 148, 241, 430, 1249, 1647, 2661, 2689
Bsp19I CCATGG 1 cut(s) 2305
BspACI CCGC 3 cut(s) 1473, 1715, 2451
BspANI GGCC 3 cut(s) 1237, 1406, 2269
BspCNI CTCAG 3 cut(s) 55, 76, 305
BspFNI CGCG 1 cut(s) 186
BspLI GGNNCC 8 cut(s) 933, 1152, 1323, 1345, 1719, 1734, 1852, 2044
BspMAI CTGCAG 1 cut(s) 918
BspMI ACCTGC 2 cut(s) 224, 1620
BspPI GGATC 4 cut(s) 70, 156, 438, 2656
BspQI GCTCTTC 2 cut(s) 2400, 2472
BspT107I GGYRCC 3 cut(s) 931, 1150, 1732
BspTI CTTAAG 1 cut(s) 1651
BsrDI GCAATG 1 cut(s) 2651
BsrGI TGTACA 3 cut(s) 552, 565, 2151
BsrI ACTGG 2 cut(s) 1053, 2456
BssECI CCNNGG 4 cut(s) 1238, 1910, 2046, 2305
BssMI GATC 8 cut(s) 75, 148, 241, 430, 1249, 1647, 2661, 2689
BssNI GRCGYC 1 cut(s) 932
BssT1I CCWWGG 2 cut(s) 1238, 2305
Bst2UI CCWGG 5 cut(s) 797, 1184, 1593, 1911, 2047
Bst4CI ACNGT 7 cut(s) 87, 143, 583, 1459, 2284, 2331, 2717
Bst6I CTCTTC 6 cut(s) 213, 401, 879, 2235, 2400, 2472
BstACI GRCGYC 1 cut(s) 932
BstAFI CTTAAG 1 cut(s) 1651
BstAUI TGTACA 3 cut(s) 552, 565, 2151
BstBAI YACGTR 1 cut(s) 2252
BstC8I GCNNGC 2 cut(s) 976, 2587
BstDEI CTNAG 5 cut(s) 42, 63, 292, 1086, 1737
BstDSI CCRYGG 1 cut(s) 2305
BstENI CCTNNNNNAGG 1 cut(s) 1740
BstF5I GGATG 7 cut(s) 495, 1081, 1216, 1261, 1764, 1921, 2227
BstFNI CGCG 1 cut(s) 186
BstH2I RGCGCY 1 cut(s) 935
BstHHI GCGC 2 cut(s) 649, 934
BstKTI GATC 8 cut(s) 78, 151, 244, 433, 1252, 1650, 2664, 2692
BstMAI GTCTC 7 cut(s) 59, 797, 1044, 1162, 1557, 2002, 2110
BstMBI GATC 8 cut(s) 75, 148, 241, 430, 1249, 1647, 2661, 2689
BstMWI GCNNNNNNNGC 3 cut(s) 777, 780, 2650
BstNI CCWGG 5 cut(s) 797, 1184, 1593, 1911, 2047
BstNSI RCATGY 5 cut(s) 553, 978, 1146, 1834, 2114
BstSCI CCNGG 5 cut(s) 795, 1182, 1591, 1909, 2045
BstSFI CTRYAG 5 cut(s) 859, 914, 1434, 1621, 2323
BstUI CGCG 1 cut(s) 186
BstV1I GCAGC 9 cut(s) 403, 451, 505, 783, 792, 795, 900, 1536, 2178
BstV2I GAAGAC 3 cut(s) 460, 855, 1833
BstX2I RGATCY 2 cut(s) 75, 1647
BstYI RGATCY 2 cut(s) 75, 1647
BsuRI GGCC 3 cut(s) 1237, 1406, 2269
BtgI CCRYGG 1 cut(s) 2305
BtsCI GGATG 7 cut(s) 495, 1081, 1216, 1261, 1764, 1921, 2227
BtsI GCAGTG 2 cut(s) 2355, 2559
BtsIMutI CAGTG 3 cut(s) 2355, 2559, 2685
BveI ACCTGC 2 cut(s) 224, 1620
Cac8I GCNNGC 2 cut(s) 976, 2587
CfoI GCGC 2 cut(s) 649, 934
Cfr13I GGNCC 2 cut(s) 1118, 1343
CseI GACGC 1 cut(s) 814
CsiI ACCWGGT 2 cut(s) 795, 1182
CspCI CAANNNNNGTGG 2 cut(s) 464, 499
DdeI CTNAG 5 cut(s) 42, 63, 292, 1086, 1737
DinI GGCGCC 1 cut(s) 933
DpnI GATC 8 cut(s) 77, 150, 243, 432, 1251, 1649, 2663, 2691
DpnII GATC 8 cut(s) 75, 148, 241, 430, 1249, 1647, 2661, 2689
EaeI YGGCCR 1 cut(s) 2267
Eam1104I CTCTTC 6 cut(s) 213, 401, 879, 2235, 2400, 2472
EarI CTCTTC 6 cut(s) 213, 401, 879, 2235, 2400, 2472
EciI GGCGGA 1 cut(s) 1488
Eco130I CCWWGG 2 cut(s) 1238, 2305
Eco147I AGGCCT 1 cut(s) 1237
Eco24I GRGCYC 2 cut(s) 1634, 1709
Eco32I GATATC 2 cut(s) 1889, 2071
Eco47I GGWCC 2 cut(s) 1118, 1343
Eco57I CTGAAG 3 cut(s) 425, 1359, 2183
Eco88I CYCGRG 1 cut(s) 24
EcoNI CCTNNNNNAGG 1 cut(s) 1740
EcoO109I RGGNCCY 2 cut(s) 1118, 1343
EcoRI GAATTC 1 cut(s) 314
EcoRII CCWGG 5 cut(s) 795, 1182, 1591, 1909, 2045
EcoRV GATATC 2 cut(s) 1889, 2071
EcoT14I CCWWGG 2 cut(s) 1238, 2305
EcoT22I ATGCAT 1 cut(s) 2378
EcoT38I GRGCYC 2 cut(s) 1634, 1709
EgeI GGCGCC 1 cut(s) 933
EheI GGCGCC 1 cut(s) 933
ErhI CCWWGG 2 cut(s) 1238, 2305
Esp3I CGTCTC 2 cut(s) 59, 797
FalI AAGNNNNNCTT 6 cut(s) 303, 335, 1818, 1850, 2427, 2459
FaqI GGGAC 3 cut(s) 521, 1329, 2521
FauNDI CATATG 1 cut(s) 2605
FbaI TGATCA 1 cut(s) 2689
FblI GTMKAC 1 cut(s) 1603
Fnu4HI GCNGC 9 cut(s) 417, 440, 494, 772, 781, 784, 914, 1550, 2192
FokI GGATG 7 cut(s) 502, 1068, 1223, 1268, 1771, 1928, 2234
FriOI GRGCYC 2 cut(s) 1634, 1709
Fsp4HI GCNGC 9 cut(s) 417, 440, 494, 772, 781, 784, 914, 1550, 2192
FspBI CTAG 7 cut(s) 1100, 1599, 1995, 2105, 2201, 2277, 2508
GlaI GCGC 2 cut(s) 648, 933
GluI GCNGC 9 cut(s) 417, 440, 494, 772, 781, 784, 914, 1550, 2192
GsaI CCCAGC 1 cut(s) 1108
HaeII RGCGCY 1 cut(s) 935
HaeIII GGCC 3 cut(s) 1237, 1406, 2269
HgaI GACGC 1 cut(s) 814
HhaI GCGC 2 cut(s) 649, 934
Hin1I GRCGYC 1 cut(s) 932
Hin6I GCGC 2 cut(s) 647, 932
HinP1I GCGC 2 cut(s) 647, 932
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HindIII AAGCTT 1 cut(s) 2441
Hpy166II GTNNAC 9 cut(s) 340, 566, 594, 793, 1293, 1604, 1768, 2172, 2685
Hpy8I GTNNAC 9 cut(s) 340, 566, 594, 793, 1293, 1604, 1768, 2172, 2685
Hpy99I CGWCG 2 cut(s) 147, 453
HpyAV CCTTC 9 cut(s) 150, 265, 298, 708, 1294, 1334, 1480, 1502, 2239
HpyCH4III ACNGT 7 cut(s) 87, 143, 583, 1459, 2284, 2331, 2717
HpyCH4IV ACGT 3 cut(s) 52, 172, 2251
HpyF10VI GCNNNNNNNGC 3 cut(s) 777, 780, 2650
HpyF3I CTNAG 5 cut(s) 42, 63, 292, 1086, 1737
HpySE526I ACGT 3 cut(s) 52, 172, 2251
Hsp92I GRCGYC 1 cut(s) 932
HspAI GCGC 2 cut(s) 647, 932
KasI GGCGCC 1 cut(s) 931
KpnI GGTACC 1 cut(s) 1736
Ksp22I TGATCA 1 cut(s) 2689
Kzo9I GATC 8 cut(s) 75, 148, 241, 430, 1249, 1647, 2661, 2689
LguI GCTCTTC 2 cut(s) 2400, 2472
LmnI GCTCC 6 cut(s) 19, 1303, 1327, 1595, 1996, 2650
Lsp1109I GCAGC 9 cut(s) 403, 451, 505, 783, 792, 795, 900, 1536, 2178
LweI GCATC 3 cut(s) 480, 1959, 2494
MabI ACCWGGT 2 cut(s) 795, 1182
MaeI CTAG 7 cut(s) 1100, 1599, 1995, 2105, 2201, 2277, 2508
MaeII ACGT 3 cut(s) 52, 172, 2251
MaeIII GTNAC 8 cut(s) 5, 236, 901, 1132, 1178, 1217, 1258, 2133
MalI GATC 8 cut(s) 77, 150, 243, 432, 1251, 1649, 2663, 2691
MboI GATC 8 cut(s) 75, 148, 241, 430, 1249, 1647, 2661, 2689
MfeI CAATTG 3 cut(s) 434, 629, 879
MflI RGATCY 2 cut(s) 75, 1647
MhlI GDGCHC 4 cut(s) 1308, 1634, 1709, 2103
MlsI TGGCCA 1 cut(s) 2269
MluI ACGCGT 1 cut(s) 184
MluNI TGGCCA 1 cut(s) 2269
Mly113I GGCGCC 1 cut(s) 932
MlyI GAGTC 3 cut(s) 380, 647, 1911
MmeI TCCRAC 5 cut(s) 103, 308, 2010, 2194, 2497
Mox20I TGGCCA 1 cut(s) 2269
Mph1103I ATGCAT 1 cut(s) 2378
MroXI GAANNNNTTC 1 cut(s) 2284
MscI TGGCCA 1 cut(s) 2269
MseI TTAA 7 cut(s) 249, 1328, 1443, 1515, 1523, 1652, 2639
MslI CAYNNNNRTG 4 cut(s) 554, 1176, 2003, 2400
Msp20I TGGCCA 1 cut(s) 2269
MspCI CTTAAG 1 cut(s) 1651
MspR9I CCNGG 5 cut(s) 797, 1184, 1593, 1911, 2047
MunI CAATTG 3 cut(s) 434, 629, 879
Mva1269I GAATGC 1 cut(s) 2378
MvaI CCWGG 5 cut(s) 797, 1184, 1593, 1911, 2047
MvnI CGCG 1 cut(s) 186
MwoI GCNNNNNNNGC 3 cut(s) 777, 780, 2650
NarI GGCGCC 1 cut(s) 932
NcoI CCATGG 1 cut(s) 2305
NdeI CATATG 1 cut(s) 2605
NdeII GATC 8 cut(s) 75, 148, 241, 430, 1249, 1647, 2661, 2689
NlaIV GGNNCC 8 cut(s) 933, 1152, 1323, 1345, 1719, 1734, 1852, 2044
NmuCI GTSAC 7 cut(s) 5, 236, 901, 1132, 1178, 1217, 1258
NsiI ATGCAT 1 cut(s) 2378
NspI RCATGY 5 cut(s) 553, 978, 1146, 1834, 2114
OliI CACNNNNGTG 1 cut(s) 1176
PaeI GCATGC 1 cut(s) 978
PaeR7I CTCGAG 1 cut(s) 24
PaqCI CACCTGC 1 cut(s) 224
PceI AGGCCT 1 cut(s) 1237
PciI ACATGT 1 cut(s) 2110
PciSI GCTCTTC 2 cut(s) 2400, 2472
PctI GAATGC 1 cut(s) 2378
PdmI GAANNNNTTC 1 cut(s) 2284
PfoI TCCNGGA 1 cut(s) 1591
PkrI GCNGC 9 cut(s) 418, 441, 495, 773, 782, 785, 915, 1551, 2193
PleI GAGTC 3 cut(s) 379, 646, 1910
PluTI GGCGCC 1 cut(s) 935
PpsI GAGTC 3 cut(s) 379, 646, 1910
Ppu21I YACGTR 1 cut(s) 2252
PpuMI RGGWCCY 2 cut(s) 1118, 1343
PscI ACATGT 1 cut(s) 2110
PshBI ATTAAT 1 cut(s) 249
PsiI TTATAA 1 cut(s) 1095
Psp5II RGGWCCY 2 cut(s) 1118, 1343
Psp6I CCWGG 5 cut(s) 795, 1182, 1591, 1909, 2045
PspFI CCCAGC 1 cut(s) 1104
PspGI CCWGG 5 cut(s) 795, 1182, 1591, 1909, 2045
PspN4I GGNNCC 8 cut(s) 933, 1152, 1323, 1345, 1719, 1734, 1852, 2044
PspPI GGNCC 2 cut(s) 1118, 1343
PspPPI RGGWCCY 2 cut(s) 1118, 1343
PspXI VCTCGAGB 1 cut(s) 24
PstI CTGCAG 1 cut(s) 918
PsuI RGATCY 2 cut(s) 75, 1647
RseI CAYNNNNRTG 4 cut(s) 554, 1176, 2003, 2400
SapI GCTCTTC 2 cut(s) 2400, 2472
SaqAI TTAA 7 cut(s) 249, 1328, 1443, 1515, 1523, 1652, 2639
SatI GCNGC 9 cut(s) 417, 440, 494, 772, 781, 784, 914, 1550, 2192
Sau3AI GATC 8 cut(s) 75, 148, 241, 430, 1249, 1647, 2661, 2689
Sau96I GGNCC 2 cut(s) 1118, 1343
SchI GAGTC 3 cut(s) 380, 647, 1911
ScrFI CCNGG 5 cut(s) 797, 1184, 1593, 1911, 2047
SduI GDGCHC 4 cut(s) 1308, 1634, 1709, 2103
SexAI ACCWGGT 2 cut(s) 795, 1182
SfaNI GCATC 3 cut(s) 480, 1959, 2494
SfcI CTRYAG 5 cut(s) 859, 914, 1434, 1621, 2323
SfoI GGCGCC 1 cut(s) 933
Sfr274I CTCGAG 1 cut(s) 24
SinI GGWCC 2 cut(s) 1118, 1343
SlaI CTCGAG 1 cut(s) 24
SmiMI CAYNNNNRTG 4 cut(s) 554, 1176, 2003, 2400
SmlI CTYRAG 7 cut(s) 24, 97, 607, 813, 1122, 1651, 2126
SmoI CTYRAG 7 cut(s) 24, 97, 607, 813, 1122, 1651, 2126
SphI GCATGC 1 cut(s) 978
SseBI AGGCCT 1 cut(s) 1237
SsiI CCGC 3 cut(s) 1473, 1715, 2451
SspDI GGCGCC 1 cut(s) 931
SspMI CTAG 7 cut(s) 1100, 1599, 1995, 2105, 2201, 2277, 2508
StuI AGGCCT 1 cut(s) 1237
StyD4I CCNGG 5 cut(s) 795, 1182, 1591, 1909, 2045
StyI CCWWGG 2 cut(s) 1238, 2305
TaaI ACNGT 7 cut(s) 87, 143, 583, 1459, 2284, 2331, 2717
TaiI ACGT 3 cut(s) 55, 175, 2254
TatI WGTACW 7 cut(s) 552, 565, 591, 1800, 2151, 2171, 2600
Tru1I TTAA 7 cut(s) 249, 1328, 1443, 1515, 1523, 1652, 2639
Tru9I TTAA 7 cut(s) 249, 1328, 1443, 1515, 1523, 1652, 2639
TscAI CASTG 3 cut(s) 2362, 2559, 2692
TseFI GTSAC 7 cut(s) 5, 236, 901, 1132, 1178, 1217, 1258
TseI GCWGC 9 cut(s) 416, 439, 493, 771, 780, 783, 913, 1549, 2191
Tsp45I GTSAC 7 cut(s) 5, 236, 901, 1132, 1178, 1217, 1258
TspGWI ACGGA 2 cut(s) 956, 1314
TspRI CASTG 3 cut(s) 2362, 2559, 2692
Vha464I CTTAAG 1 cut(s) 1651
VpaK11BI GGWCC 2 cut(s) 1118, 1343
VspI ATTAAT 1 cut(s) 249
XagI CCTNNNNNAGG 1 cut(s) 1740
XapI RAATTY 3 cut(s) 279, 314, 1357
XceI RCATGY 5 cut(s) 553, 978, 1146, 1834, 2114
XhoI CTCGAG 1 cut(s) 24
XmiI GTMKAC 1 cut(s) 1603
XmnI GAANNNNTTC 1 cut(s) 2284
XspI CTAG 7 cut(s) 1100, 1599, 1995, 2105, 2201, 2277, 2508
Zsp2I ATGCAT 1 cut(s) 2378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.