Rw4G030750
ERF Family

DNA RNA polymerases superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000291
Physical Location & Seq
Forward (+)
57439569 .. 57440923
1355 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G030750.1

Sequence Viewer

Length: 1317 bp
ATGGGTGAAGTTACGAAATCAGATATTGAAGCTCTTACAGCAGCGTTTACCGCTGCCATCAATTCCATGAATAATCAGATTGGAGAAATTCGTGGATTGTTGGATGAGAGGAACAACAATAATAATCGGCATAGAGGTGGGGAAGGAGGCCAGCGAGTTAGGGCTCCACGTGGTGAAGTTGTTGATAATACATCAGGATCTGAGCCTGAAGAAGAAATTTTACAACAAGAGCAACAGGGACAAGCTGACCAGGATTACAAAGTCAAGGCCGAAATTCCTTTCTTTTCGGGCAATTTGGGTGTAGAAGATTATTTGGATTGGCAGCTTCAGGTGGACAGATTCTTTGAGATTATGGAAGTGCCTGAACACAAGCAAGTTAAGCATGTTGCCTGGAGATTGAAGAGTACTGCTGCAGTATGGTGGGATAAGTTGCAGAACACTCGAAAGAAGCAGAGGAAGCAGGGTGTTAGAACATGGCGAAGAATGAAGCAGTTGATGATGGAGAGGTTCTTGCCAGATGATTATGAGCAAATTCTATACAGGCTGTATATTGAATGTGTCCAAGGAACTAGGACGGTGGCTGAATATACAGCTGAATTCTTACGCTATTCGGAGCGTAATGAATTAGGAGAAACTGAAGGCCAGAAGGTGGCTCGCTATATCAGTGGGCTGAAGCCTTCCATTCAGGAGAGAATTGGGTTACAAACTGTTCATACTATGGCCGAAAGGTATAACTACCAAAGGAGTGCAGATTTGGTTAATGCCTCAACTGCCAAGGGTACAACCACGCTGCAGAGAGCAGAAAACTCTTTTAAAGCTTCTAATCCTCCTTTTAAAGGCGCGGGCAGTTCTAACAGTTCTAGTGGTGCTCAAAATAGAGCCCCGAATCAGAGGTTTAATAATCCTTATGCTAGACCTACAGGAGATGTCTGTTATCGATGCAACAAGCCTGGGCATAGATCTAATGTGTGTCCTGAGAGGAGACAAACTGCCCTTATAGATGATTATGATGAGGATGAAGTAGAAGTTGGAAGAGATGGCGATTATGATGGGGCTGAGTTTGCTGAGGAGGAATCCCCTGAGAAGGTTAATATTGTGTTGCAGCGGGTTCTACTATGTCCTAAAGAAGAGAGGCAGCGGCGCAATATTTTCAGGTCACTTTGTTCCATTAATAACAAAGTGTGCAATTTAATTGTGGATAATGGAAGCTGCGAAAATTTTGTAGCCAAGAAACTGGTGGAGTATTTGCAGTTACCTACGCAGCCTCATGAGGCACCTTATTCCCTCGGGTGGGTCAAGAAAGGTCCACAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 Retrotrans_gag rve zf-H2C2

Protein Analysis

438

Amino Acids

50.1

Weight (kDa)

6.4

Isoelectric Point (pI)

45.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ty3_capsid PF19259 116 - 246 1.2e-06 Ty3 transposon capsid-like protein
Retrotrans_gag PF03732 128 - 226 6e-14 Retrotransposon gag protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000291)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG00860
fragaria_vesca FvH4_1g23712 FvH4_1g29809 FvH4_1g29810 FvH4_2g02661 FvH4_2g13451 FvH4_2g17921 FvH4_2g17922 FvH4_2g19251 FvH4_2g19252 FvH4_2g28221 FvH4_2g35961 FvH4_3g10372 FvH4_3g12461 FvH4_3g12461 FvH4_3g27861 FvH4_3g34591 FvH4_3g40201 FvH4_3g44612 FvH4_4g08352 FvH4_4g12251 FvH4_4g12742 FvH4_4g13904 FvH4_4g14287 FvH4_4g15243 FvH4_4g24071 FvH4_4g33448 FvH4_5g21912 FvH4_5g39371 FvH4_6g14992 FvH4_6g15221 FvH4_6g20603 FvH4_6g31093 FvH4_6g32372 FvH4_6g33012
malus_domestica MD11G1009100.v1.1 MD13G1145000.v1.1 MD16G1138700.v1.1
prunus_persica Prupe.5G246300_v2.0.a1 Prupe.7G034800_v2.0.a1 Prupe.8G084500_v2.0.a1
pyrus_communis pycom01g18240 pycom04g00330 pycom05g09010 pycom06g01670 pycom06g01690 pycom06g07180 pycom06g11620 pycom10g05720 pycom1134g00040 pycom1134g00060 pycom12486g00010 pycom12555g00230 pycom13g21670 pycom13g23130 pycom13g23140 pycom14g10580
rosa_chinensis RchiOBHm_Chr1g0330691 RchiOBHm_Chr2g0106171 RchiOBHm_Chr2g0134731 RchiOBHm_Chr2g0142791 RchiOBHm_Chr2g0149861 RchiOBHm_Chr3g0452401 RchiOBHm_Chr3g0461881 RchiOBHm_Chr4g0401341 RchiOBHm_Chr5g0075641 RchiOBHm_Chr6g0265961 RchiOBHm_Chr7g0200201 RchiOBHm_Chr7g0237811
rosa_laevigata RLG00000002773
rosa_multiflora Rmu_co8037806.1_g000001 Rmu_co8445989.1_g000001 Rmu_sc0000745.1_g000039 Rmu_sc0004448.1_g000029 Rmu_sc0004488.1_g000002 Rmu_sc0004591.1_g000018 Rmu_sc0005065.1_g000030 Rmu_sc0005150.1_g000005 Rmu_sc0005654.1_g000011 Rmu_sc0006986.1_g000016
rosa_roxburghii Rroxscaffold_164G00436490 Rroxscaffold_175G00432490 Rroxscaffold_1G00059440 Rroxscaffold_1G00064410 Rroxscaffold_2G00100310 Rroxscaffold_2G00128170 Rroxscaffold_3G00239420 Rroxscaffold_3G00264970 Rroxscaffold_3G00271550 Rroxscaffold_4G00300240 Rroxscaffold_5G00385050 Rroxscaffold_6G00399720
rosa_rugosa Rorug02G0252200
rosa_samantha Rh2DG251900 Rh7CG329900
rosa_wichuraiana Rw0G004800 Rw1G000850 Rw1G022350 Rw2G027030 Rw2G027760 Rw2G028410 Rw2G029130 Rw2G029390 Rw2G032490 Rw2G039460 Rw2G047800 Rw2G050340 Rw3G026430 Rw4G018370 Rw4G030750 Rw4G033140 Rw5G011950 Rw5G012010 Rw5G029280 Rw5G040190 Rw5G044570 Rw5G048240 Rw6G000340 Rw6G010140 Rw6G026480 Rw7G033750 Rw7G037250 Rw7G042080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1273
AccB7I CCANNNNNTGG 1 cut(s) 649
AccII CGCG 1 cut(s) 842
AciI CCGC 4 cut(s) 51, 842, 1105, 1138
AclWI GGATC 1 cut(s) 205
AcoI YGGCCR 1 cut(s) 720
AcsI RAATTY 6 cut(s) 87, 216, 273, 531, 596, 1216
AcuI CTGAAG 4 cut(s) 228, 311, 657, 692
AcvI CACGTG 1 cut(s) 170
AdeI CACNNNGTG 1 cut(s) 173
AfaI GTAC 2 cut(s) 406, 781
AfiI CCNNNNNNNGG 5 cut(s) 649, 836, 1084, 1290, 1291
AgsI TTSAA 3 cut(s) 29, 400, 554
AjnI CCWGG 3 cut(s) 249, 389, 949
AjuI GAANNNNNNNTTGG 2 cut(s) 1011, 1043
AluBI AGCT 6 cut(s) 32, 245, 325, 593, 818, 1209
AluI AGCT 6 cut(s) 32, 245, 325, 593, 818, 1209
Alw21I GWGCWC 1 cut(s) 871
Alw26I GTCTC 1 cut(s) 976
AlwI GGATC 1 cut(s) 205
AlwNI CAGNNNCTG 1 cut(s) 200
Ama87I CYCGRG 1 cut(s) 1286
AoxI GGCC 4 cut(s) 148, 267, 640, 720
ApeKI GCWGC 9 cut(s) 41, 53, 322, 410, 790, 1102, 1135, 1209, 1261
ApoI RAATTY 6 cut(s) 87, 216, 273, 531, 596, 1216
ArsI GACNNNNNNTTYG 2 cut(s) 326, 358
AseI ATTAAT 1 cut(s) 1170
AspLEI GCGC 2 cut(s) 842, 1143
AspS9I GGNCC 1 cut(s) 1304
AsuHPI GGTGA 2 cut(s) 17, 185
AvaI CYCGRG 1 cut(s) 1286
AvaII GGWCC 1 cut(s) 1304
BanI GGYRCC 1 cut(s) 1273
BanII GRGCYC 2 cut(s) 166, 883
BarI GAAGNNNNNNTAC 2 cut(s) 204, 236
BbrPI CACGTG 1 cut(s) 170
Bbv12I GWGCWC 1 cut(s) 871
BbvCI CCTCAGC 1 cut(s) 1065
BbvI GCAGC 9 cut(s) 40, 53, 334, 397, 777, 1114, 1147, 1196, 1273
BccI CCATC 4 cut(s) 65, 493, 1031, 1043
BcgI CGANNNNNNTGC 2 cut(s) 422, 456
BciT130I CCWGG 3 cut(s) 251, 391, 951
BcoDI GTCTC 1 cut(s) 976
BfaI CTAG 3 cut(s) 570, 861, 912
BfmI CTRYAG 3 cut(s) 411, 791, 918
BglII AGATCT 1 cut(s) 959
BmcAI AGTACT 1 cut(s) 406
Bme1390I CCNGG 3 cut(s) 251, 391, 951
Bme18I GGWCC 1 cut(s) 1304
BmeT110I CYCGRG 1 cut(s) 1286
BmgT120I GGNCC 1 cut(s) 1304
BmiI GGNNCC 2 cut(s) 165, 1275
BmrFI CCNGG 3 cut(s) 251, 391, 951
BmsI GCATC 1 cut(s) 929
BpmI CTGGAG 1 cut(s) 412
Bpu10I CCTNAGC 1 cut(s) 1065
Bsa29I ATCGAT 1 cut(s) 937
BsaAI YACGTR 1 cut(s) 170
BsaJI CCNNGG 4 cut(s) 562, 774, 950, 1285
Bsc4I CCNNNNNNNGG 5 cut(s) 649, 836, 1084, 1290, 1291
Bse1I ACTGG 1 cut(s) 1239
BseBI CCWGG 3 cut(s) 251, 391, 951
BseCI ATCGAT 1 cut(s) 937
BseDI CCNNGG 4 cut(s) 562, 774, 950, 1285
BseGI GGATG 2 cut(s) 109, 1021
BseLI CCNNNNNNNGG 5 cut(s) 649, 836, 1084, 1290, 1291
BseMII CTCAG 5 cut(s) 192, 966, 1047, 1056, 1071
BseNI ACTGG 1 cut(s) 1239
BseRI GAGGAG 2 cut(s) 994, 1082
BseXI GCAGC 9 cut(s) 40, 53, 334, 397, 777, 1114, 1147, 1196, 1273
BsgI GTGCAG 1 cut(s) 768
Bsh1236I CGCG 1 cut(s) 842
BshFI GGCC 4 cut(s) 150, 269, 642, 722
BshNI GGYRCC 1 cut(s) 1273
BshVI ATCGAT 1 cut(s) 937
BsiHKAI GWGCWC 1 cut(s) 871
BsiHKCI CYCGRG 1 cut(s) 1286
BslFI GGGAC 1 cut(s) 252
BslI CCNNNNNNNGG 5 cut(s) 649, 836, 1084, 1290, 1291
BsmAI GTCTC 1 cut(s) 976
BsmFI GGGAC 1 cut(s) 252
BsnI GGCC 4 cut(s) 150, 269, 642, 722
BsoBI CYCGRG 1 cut(s) 1286
Bsp1286I GDGCHC 3 cut(s) 166, 871, 883
Bsp143I GATC 2 cut(s) 197, 959
BspACI CCGC 4 cut(s) 51, 842, 1105, 1138
BspANI GGCC 4 cut(s) 150, 269, 642, 722
BspCNI CTCAG 5 cut(s) 193, 967, 1048, 1057, 1072
BspDI ATCGAT 1 cut(s) 937
BspFNI CGCG 1 cut(s) 842
BspHI TCATGA 1 cut(s) 1267
BspLI GGNNCC 2 cut(s) 165, 1275
BspMAI CTGCAG 2 cut(s) 415, 795
BspPI GGATC 1 cut(s) 205
BspT107I GGYRCC 1 cut(s) 1273
BsrI ACTGG 1 cut(s) 1239
BssECI CCNNGG 4 cut(s) 562, 774, 950, 1285
BssMI GATC 2 cut(s) 197, 959
BssT1I CCWWGG 2 cut(s) 562, 774
Bst2UI CCWGG 3 cut(s) 251, 391, 951
Bst4CI ACNGT 3 cut(s) 577, 709, 857
Bst6I CTCTTC 3 cut(s) 395, 1027, 1122
BstBAI YACGTR 1 cut(s) 170
BstC8I GCNNGC 3 cut(s) 152, 655, 844
BstDEI CTNAG 5 cut(s) 201, 975, 1056, 1065, 1080
BstENI CCTNNNNNAGG 1 cut(s) 834
BstF5I GGATG 2 cut(s) 109, 1021
BstFNI CGCG 1 cut(s) 842
BstHHI GCGC 2 cut(s) 842, 1143
BstKTI GATC 2 cut(s) 200, 962
BstMAI GTCTC 1 cut(s) 976
BstMBI GATC 2 cut(s) 197, 959
BstMWI GCNNNNNNNGC 6 cut(s) 38, 50, 379, 457, 770, 1061
BstNI CCWGG 3 cut(s) 251, 391, 951
BstNSI RCATGY 1 cut(s) 386
BstSCI CCNGG 3 cut(s) 249, 389, 949
BstSFI CTRYAG 3 cut(s) 411, 791, 918
BstUI CGCG 1 cut(s) 842
BstV1I GCAGC 9 cut(s) 40, 53, 334, 397, 777, 1114, 1147, 1196, 1273
BstX2I RGATCY 2 cut(s) 197, 959
BstXI CCANNNNNNTGG 1 cut(s) 1234
BstYI RGATCY 2 cut(s) 197, 959
Bsu15I ATCGAT 1 cut(s) 937
BsuRI GGCC 4 cut(s) 150, 269, 642, 722
BsuTUI ATCGAT 1 cut(s) 937
BtsCI GGATG 2 cut(s) 109, 1021
BtsIMutI CAGTG 1 cut(s) 670
Cac8I GCNNGC 3 cut(s) 152, 655, 844
CaiI CAGNNNCTG 1 cut(s) 200
CciI TCATGA 1 cut(s) 1267
CfoI GCGC 2 cut(s) 842, 1143
Cfr13I GGNCC 1 cut(s) 1304
ClaI ATCGAT 1 cut(s) 937
Csp6I GTAC 2 cut(s) 405, 780
CviAII CATG 4 cut(s) 67, 383, 474, 1268
CviQI GTAC 2 cut(s) 405, 780
DdeI CTNAG 5 cut(s) 201, 975, 1056, 1065, 1080
DpnI GATC 2 cut(s) 199, 961
DpnII GATC 2 cut(s) 197, 959
DraI TTTAAA 2 cut(s) 814, 835
DraIII CACNNNGTG 1 cut(s) 173
EaeI YGGCCR 1 cut(s) 720
Eam1104I CTCTTC 3 cut(s) 395, 1027, 1122
EarI CTCTTC 3 cut(s) 395, 1027, 1122
Eco130I CCWWGG 2 cut(s) 562, 774
Eco24I GRGCYC 2 cut(s) 166, 883
Eco47I GGWCC 1 cut(s) 1304
Eco57I CTGAAG 4 cut(s) 228, 311, 657, 692
Eco72I CACGTG 1 cut(s) 170
Eco88I CYCGRG 1 cut(s) 1286
EcoNI CCTNNNNNAGG 1 cut(s) 834
EcoRI GAATTC 1 cut(s) 596
EcoRII CCWGG 3 cut(s) 249, 389, 949
EcoT14I CCWWGG 2 cut(s) 562, 774
EcoT38I GRGCYC 2 cut(s) 166, 883
ErhI CCWWGG 2 cut(s) 562, 774
FaeI CATG 4 cut(s) 70, 386, 477, 1271
FaqI GGGAC 1 cut(s) 252
FatI CATG 4 cut(s) 66, 382, 473, 1267
FauI CCCGC 2 cut(s) 835, 1098
FokI GGATG 2 cut(s) 116, 1028
FriOI GRGCYC 2 cut(s) 166, 883
FspBI CTAG 3 cut(s) 570, 861, 912
GlaI GCGC 2 cut(s) 841, 1142
GsuI CTGGAG 1 cut(s) 412
HaeIII GGCC 4 cut(s) 150, 269, 642, 722
HhaI GCGC 2 cut(s) 842, 1143
Hin1II CATG 4 cut(s) 70, 386, 477, 1271
Hin6I GCGC 2 cut(s) 840, 1141
HinP1I GCGC 2 cut(s) 840, 1141
HindIII AAGCTT 1 cut(s) 816
HinfI GANTC 3 cut(s) 339, 886, 1073
HphI GGTGA 2 cut(s) 17, 185
Hpy166II GTNNAC 3 cut(s) 48, 334, 1307
Hpy188I TCNGA 5 cut(s) 22, 78, 202, 613, 891
Hpy188III TCNNGA 5 cut(s) 195, 686, 974, 1268, 1297
Hpy8I GTNNAC 3 cut(s) 48, 334, 1307
HpyAV CCTTC 5 cut(s) 137, 632, 640, 687, 1078
HpyCH4III ACNGT 3 cut(s) 577, 709, 857
HpyCH4IV ACGT 1 cut(s) 169
HpyCH4V TGCA 8 cut(s) 413, 433, 749, 793, 942, 1102, 1185, 1249
HpyF10VI GCNNNNNNNGC 6 cut(s) 38, 50, 379, 457, 770, 1061
HpyF3I CTNAG 5 cut(s) 201, 975, 1056, 1065, 1080
HpySE526I ACGT 1 cut(s) 169
Hsp92II CATG 4 cut(s) 70, 386, 477, 1271
HspAI GCGC 2 cut(s) 840, 1141
Kzo9I GATC 2 cut(s) 197, 959
LmnI GCTCC 2 cut(s) 169, 613
Lsp1109I GCAGC 9 cut(s) 40, 53, 334, 397, 777, 1114, 1147, 1196, 1273
LweI GCATC 1 cut(s) 929
MaeI CTAG 3 cut(s) 570, 861, 912
MaeII ACGT 1 cut(s) 169
MaeIII GTNAC 4 cut(s) 10, 699, 1155, 1251
MalI GATC 2 cut(s) 199, 961
MboI GATC 2 cut(s) 197, 959
MboII GAAGA 7 cut(s) 221, 224, 317, 412, 492, 1044, 1139
MflI RGATCY 2 cut(s) 197, 959
MhlI GDGCHC 3 cut(s) 166, 871, 883
MmeI TCCRAC 2 cut(s) 81, 1009
MseI TTAA 8 cut(s) 378, 759, 813, 834, 897, 1089, 1170, 1190
MslI CAYNNNNRTG 1 cut(s) 135
MspA1I CMGCKG 4 cut(s) 53, 593, 1105, 1138
MspR9I CCNGG 3 cut(s) 251, 391, 951
MvaI CCWGG 3 cut(s) 251, 391, 951
MvnI CGCG 1 cut(s) 842
MwoI GCNNNNNNNGC 6 cut(s) 38, 50, 379, 457, 770, 1061
NdeII GATC 2 cut(s) 197, 959
NlaIII CATG 4 cut(s) 70, 386, 477, 1271
NlaIV GGNNCC 2 cut(s) 165, 1275
NmuCI GTSAC 1 cut(s) 1155
NspI RCATGY 1 cut(s) 386
PagI TCATGA 1 cut(s) 1267
PfeI GAWTC 3 cut(s) 339, 886, 1073
PflMI CCANNNNNTGG 1 cut(s) 649
PmaCI CACGTG 1 cut(s) 170
PmlI CACGTG 1 cut(s) 170
Ppu21I YACGTR 1 cut(s) 170
PshBI ATTAAT 1 cut(s) 1170
Psp6I CCWGG 3 cut(s) 249, 389, 949
PspCI CACGTG 1 cut(s) 170
PspGI CCWGG 3 cut(s) 249, 389, 949
PspN4I GGNNCC 2 cut(s) 165, 1275
PspPI GGNCC 1 cut(s) 1304
PstI CTGCAG 2 cut(s) 415, 795
PstNI CAGNNNCTG 1 cut(s) 200
PsuI RGATCY 2 cut(s) 197, 959
PvuII CAGCTG 1 cut(s) 593
RsaI GTAC 2 cut(s) 406, 781
RsaNI GTAC 2 cut(s) 405, 780
RseI CAYNNNNRTG 1 cut(s) 135
SaqAI TTAA 8 cut(s) 378, 759, 813, 834, 897, 1089, 1170, 1190
Sau3AI GATC 2 cut(s) 197, 959
Sau96I GGNCC 1 cut(s) 1304
ScaI AGTACT 1 cut(s) 406
ScrFI CCNGG 3 cut(s) 251, 391, 951
SduI GDGCHC 3 cut(s) 166, 871, 883
SfaNI GCATC 1 cut(s) 929
SfcI CTRYAG 3 cut(s) 411, 791, 918
SinI GGWCC 1 cut(s) 1304
SmiMI CAYNNNNRTG 1 cut(s) 135
SsiI CCGC 4 cut(s) 51, 842, 1105, 1138
SspI AATATT 2 cut(s) 1093, 1147
SspMI CTAG 3 cut(s) 570, 861, 912
StyD4I CCNGG 3 cut(s) 249, 389, 949
StyI CCWWGG 2 cut(s) 562, 774
TaaI ACNGT 3 cut(s) 577, 709, 857
TaiI ACGT 1 cut(s) 172
TaqI TCGA 2 cut(s) 442, 937
TatI WGTACW 1 cut(s) 404
TauI GCSGC 1 cut(s) 1141
TfiI GAWTC 3 cut(s) 339, 886, 1073
Tru1I TTAA 8 cut(s) 378, 759, 813, 834, 897, 1089, 1170, 1190
Tru9I TTAA 8 cut(s) 378, 759, 813, 834, 897, 1089, 1170, 1190
TscAI CASTG 1 cut(s) 670
TseFI GTSAC 1 cut(s) 1155
TseI GCWGC 9 cut(s) 41, 53, 322, 410, 790, 1102, 1135, 1209, 1261
Tsp45I GTSAC 1 cut(s) 1155
TspDTI ATGAA 5 cut(s) 83, 500, 636, 701, 1032
TspRI CASTG 1 cut(s) 670
Van91I CCANNNNNTGG 1 cut(s) 649
VpaK11BI GGWCC 1 cut(s) 1304
VspI ATTAAT 1 cut(s) 1170
XagI CCTNNNNNAGG 1 cut(s) 834
XapI RAATTY 6 cut(s) 87, 216, 273, 531, 596, 1216
XceI RCATGY 1 cut(s) 386
XcmI CCANNNNNNNNNTGG 1 cut(s) 1234
XspI CTAG 3 cut(s) 570, 861, 912
ZrmI AGTACT 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.