FvH4_2g19252

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000291
Physical Location & Seq
Forward (+)
16405542 .. 16406840
1299 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g19252.t1

Sequence Viewer

Length: 1299 bp
ATGGTTCGATGCAGTATTGATGAACCGGAAGAGCAAACTATTGCCCGTTATTTGGGAGGGTTACGGAAGGAGATTCATGATGTGGTAACATTGCAGCCTTATTGGTCATATGATGATGTCTACAAGCTTGCAGTCAAAGTAGAGAAGCAATTGAAGCAGAAGGCTAGTCGCACGGCAAGTGGATTTGGTCCTTGCAACCCTATTAGAAAGGAACAGCCTTTTGTATCTGTTCGGGAACCCGAAAAGCCGATTCCAAGGCATTTTGAGAAGGGAGCCGATGGTAAGAGAGATGGCAGCTCTAGTTCTAGTCGGTCAACCGGTTTTGGCAACCGAATGGTGAAATGTTTCAAGTGCTCAGGTTTGGGGCATGTGCAAGCTGATTGTCCAAATAAGAGTTTTGTCAACTTTGCAAGTGAGGCTCTGGTTTCGGATGACTCAACCAAAGAAGATGATACTCCTATATATGATGAATACAATGATGATGATGATGACGGTGTTACATGGAGTGATCATGGAAAGGCCTTAGTCATTCAACGAAGCATGAATGCTTCAAGAGTTGAGGACCATGACTGGTTGCGCCACAACATTTTTCATACTCGGTGCACTGCCAATGGGAAGATATGCAATGTAATCATAGATGGTGGAAGTTCAGAAAATGTTGTGTCACAAGAAATGGTTGACAAGCTAAGGTTGAGGGTCGAGAAACGCCCAACTTCATACAAGCTTGCTTGGTTCAAGAAAGGAAGTGAGGTAAATGTTGATAAACGTTGTTTAGTTTCATTTTCTATTGGCAAATTGTATCAAGACAAAGTGTGGTGCGATGTGGTACCTATGGATGCATGTCACTTACTGTTAGGGAGGCCATGGCAGTATGATCGAAAAGCTTTCCATGATGGGTACAAGAACACTTATACTTTTGTGAAGGATGGTGTTAAGGTGATTTTGGGGCCTTCAAGAAGTGATCAGACAGTAGCCAAGTGCAACACTCAAGAGAGGGCTACCTTCCTTACCATGACAGAATTTGTAGAAGAGATTCAAGGAGAAGCTCAAGTGTATGCGTTGGTGGTGCAAGAGGTGAATGCTGGAAGTCCAATTCCTCAACGGCTTGAACCTTTATTCAAAGAGTTTTCTGATGTTATACCTGAGGAGTTACCAAAAGGCTTACCACCTATGAGGGATGTACAACACCAAATCGACTTCATTCCAGGATCTAATTTACTGAACAAAGCTGCTTACCATATGAGCCCAGCCGAGCATCTTGAGCTGCAAAGGCAAATTAGAGAGTTAGTTGAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 Retrotrans_gag rve zf-CCHC zf-H2C2

Protein Analysis

433

Amino Acids

49.05

Weight (kDa)

6.21

Isoelectric Point (pI)

43.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp_protease_2 PF13650 201 - 289 9.3e-06 Aspartyl protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000291)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG00860
fragaria_vesca FvH4_1g23712 FvH4_1g29809 FvH4_1g29810 FvH4_2g02661 FvH4_2g13451 FvH4_2g17921 FvH4_2g17922 FvH4_2g19251 FvH4_2g19252 FvH4_2g28221 FvH4_2g35961 FvH4_3g10372 FvH4_3g12461 FvH4_3g12461 FvH4_3g27861 FvH4_3g34591 FvH4_3g40201 FvH4_3g44612 FvH4_4g08352 FvH4_4g12251 FvH4_4g12742 FvH4_4g13904 FvH4_4g14287 FvH4_4g15243 FvH4_4g24071 FvH4_4g33448 FvH4_5g21912 FvH4_5g39371 FvH4_6g14992 FvH4_6g15221 FvH4_6g20603 FvH4_6g31093 FvH4_6g32372 FvH4_6g33012
malus_domestica MD11G1009100.v1.1 MD13G1145000.v1.1 MD16G1138700.v1.1
prunus_persica Prupe.5G246300_v2.0.a1 Prupe.7G034800_v2.0.a1 Prupe.8G084500_v2.0.a1
pyrus_communis pycom01g18240 pycom04g00330 pycom05g09010 pycom06g01670 pycom06g01690 pycom06g07180 pycom06g11620 pycom10g05720 pycom1134g00040 pycom1134g00060 pycom12486g00010 pycom12555g00230 pycom13g21670 pycom13g23130 pycom13g23140 pycom14g10580
rosa_chinensis RchiOBHm_Chr1g0330691 RchiOBHm_Chr2g0106171 RchiOBHm_Chr2g0134731 RchiOBHm_Chr2g0142791 RchiOBHm_Chr2g0149861 RchiOBHm_Chr3g0452401 RchiOBHm_Chr3g0461881 RchiOBHm_Chr4g0401341 RchiOBHm_Chr5g0075641 RchiOBHm_Chr6g0265961 RchiOBHm_Chr7g0200201 RchiOBHm_Chr7g0237811
rosa_laevigata RLG00000002773
rosa_multiflora Rmu_co8037806.1_g000001 Rmu_co8445989.1_g000001 Rmu_sc0000745.1_g000039 Rmu_sc0004448.1_g000029 Rmu_sc0004488.1_g000002 Rmu_sc0004591.1_g000018 Rmu_sc0005065.1_g000030 Rmu_sc0005150.1_g000005 Rmu_sc0005654.1_g000011 Rmu_sc0006986.1_g000016
rosa_roxburghii Rroxscaffold_164G00436490 Rroxscaffold_175G00432490 Rroxscaffold_1G00059440 Rroxscaffold_1G00064410 Rroxscaffold_2G00100310 Rroxscaffold_2G00128170 Rroxscaffold_3G00239420 Rroxscaffold_3G00264970 Rroxscaffold_3G00271550 Rroxscaffold_4G00300240 Rroxscaffold_5G00385050 Rroxscaffold_6G00399720
rosa_rugosa Rorug02G0252200
rosa_samantha Rh2DG251900 Rh7CG329900
rosa_wichuraiana Rw0G004800 Rw1G000850 Rw1G022350 Rw2G027030 Rw2G027760 Rw2G028410 Rw2G029130 Rw2G029390 Rw2G032490 Rw2G039460 Rw2G047800 Rw2G050340 Rw3G026430 Rw4G018370 Rw4G030750 Rw4G033140 Rw5G011950 Rw5G012010 Rw5G029280 Rw5G040190 Rw5G044570 Rw5G048240 Rw6G000340 Rw6G010140 Rw6G026480 Rw7G033750 Rw7G037250 Rw7G042080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 826
AccB1I GGYRCC 1 cut(s) 826
AccI GTMKAC 1 cut(s) 120
AclI AACGTT 1 cut(s) 766
AclWI GGATC 1 cut(s) 1216
AcsI RAATTY 1 cut(s) 1019
AfaI GTAC 3 cut(s) 828, 899, 1182
AfiI CCNNNNNNNGG 1 cut(s) 52
AgeI ACCGGT 1 cut(s) 317
AgsI TTSAA 9 cut(s) 154, 349, 533, 552, 736, 954, 1037, 1109, 1120
AjnI CCWGG 1 cut(s) 1204
AluBI AGCT 9 cut(s) 127, 297, 377, 685, 724, 884, 1046, 1229, 1264
AluI AGCT 9 cut(s) 127, 297, 377, 685, 724, 884, 1046, 1229, 1264
Alw21I GWGCWC 2 cut(s) 356, 605
Alw44I GTGCAC 1 cut(s) 601
AlwI GGATC 1 cut(s) 1216
AoxI GGCC 3 cut(s) 519, 860, 947
ApaLI GTGCAC 1 cut(s) 601
ApeKI GCWGC 4 cut(s) 94, 294, 1229, 1264
ApoI RAATTY 1 cut(s) 1019
AsiGI ACCGGT 1 cut(s) 317
Asp700I GAANNNNTTC 2 cut(s) 344, 1032
Asp718I GGTACC 1 cut(s) 826
AspLEI GCGC 1 cut(s) 579
AspS9I GGNCC 3 cut(s) 188, 562, 947
AsuHPI GGTGA 3 cut(s) 349, 949, 1087
AvaII GGWCC 2 cut(s) 188, 562
AxyI CCTNAGG 1 cut(s) 1143
BaeGI GKGCMC 1 cut(s) 605
BanI GGYRCC 1 cut(s) 826
BanII GRGCYC 1 cut(s) 1247
Bbv12I GWGCWC 2 cut(s) 356, 605
BbvI GCAGC 4 cut(s) 106, 306, 1216, 1251
BccI CCATC 5 cut(s) 272, 284, 632, 887, 920
BceAI ACGGC 2 cut(s) 189, 1118
BcgI CGANNNNNNTGC 2 cut(s) 857, 891
BciT130I CCWGG 1 cut(s) 1206
BclI TGATCA 2 cut(s) 508, 961
BfaI CTAG 3 cut(s) 165, 300, 306
BisI GCNGC 4 cut(s) 95, 295, 1230, 1265
BlsI GCNGC 4 cut(s) 96, 296, 1231, 1266
Bme1390I CCNGG 1 cut(s) 1206
Bme18I GGWCC 2 cut(s) 188, 562
BmgT120I GGNCC 3 cut(s) 188, 562, 947
BmiI GGNNCC 4 cut(s) 237, 274, 828, 948
BmrFI CCNGG 1 cut(s) 1206
BmsI GCATC 2 cut(s) 826, 1264
Bpu10I CCTNAGC 2 cut(s) 355, 686
BpuEI CTTGAG 3 cut(s) 972, 1032, 1280
BsaJI CCNNGG 2 cut(s) 254, 863
BsaWI WCCGGW 2 cut(s) 25, 317
Bsc4I CCNNNNNNNGG 1 cut(s) 52
Bse118I RCCGGY 1 cut(s) 317
Bse1I ACTGG 1 cut(s) 575
Bse21I CCTNAGG 1 cut(s) 1143
Bse3DI GCAATG 2 cut(s) 89, 631
BseBI CCWGG 1 cut(s) 1206
BseDI CCNNGG 2 cut(s) 254, 863
BseGI GGATG 4 cut(s) 436, 841, 931, 1183
BseLI CCNNNNNNNGG 1 cut(s) 52
BseMI GCAATG 2 cut(s) 89, 631
BseMII CTCAG 2 cut(s) 369, 1134
BseNI ACTGG 1 cut(s) 575
BseRI GAGGAG 1 cut(s) 1160
BseSI GKGCMC 1 cut(s) 605
BseXI GCAGC 4 cut(s) 106, 306, 1216, 1251
BseYI CCCAGC 1 cut(s) 1246
BshFI GGCC 3 cut(s) 521, 862, 949
BshNI GGYRCC 1 cut(s) 826
BshTI ACCGGT 1 cut(s) 317
BsiHKAI GWGCWC 2 cut(s) 356, 605
BsiSI CCGG 2 cut(s) 26, 318
BslI CCNNNNNNNGG 1 cut(s) 52
BsmI GAATGC 2 cut(s) 550, 1084
BsnI GGCC 3 cut(s) 521, 862, 949
Bsp1286I GDGCHC 3 cut(s) 356, 605, 1247
Bsp1407I TGTACA 1 cut(s) 1180
Bsp143I GATC 4 cut(s) 508, 874, 961, 1208
Bsp19I CCATGG 1 cut(s) 863
BspANI GGCC 3 cut(s) 521, 862, 949
BspCNI CTCAG 2 cut(s) 368, 1135
BspHI TCATGA 1 cut(s) 76
BspLI GGNNCC 4 cut(s) 237, 274, 828, 948
BspPI GGATC 1 cut(s) 1216
BspQI GCTCTTC 1 cut(s) 24
BspT107I GGYRCC 1 cut(s) 826
BsrDI GCAATG 2 cut(s) 89, 631
BsrFI RCCGGY 1 cut(s) 317
BsrGI TGTACA 1 cut(s) 1180
BsrI ACTGG 1 cut(s) 575
BssAI RCCGGY 1 cut(s) 317
BssECI CCNNGG 2 cut(s) 254, 863
BssMI GATC 4 cut(s) 508, 874, 961, 1208
BssT1I CCWWGG 2 cut(s) 254, 863
Bst2UI CCWGG 1 cut(s) 1206
Bst4CI ACNGT 3 cut(s) 494, 852, 970
Bst6I CTCTTC 2 cut(s) 24, 1023
BstAUI TGTACA 1 cut(s) 1180
BstC8I GCNNGC 3 cut(s) 129, 375, 726
BstDEI CTNAG 4 cut(s) 355, 523, 686, 1143
BstDSI CCRYGG 1 cut(s) 863
BstF5I GGATG 4 cut(s) 436, 841, 931, 1183
BstHHI GCGC 1 cut(s) 579
BstKTI GATC 4 cut(s) 511, 877, 964, 1211
BstMBI GATC 4 cut(s) 508, 874, 961, 1208
BstMWI GCNNNNNNNGC 4 cut(s) 154, 416, 1261, 1270
BstNI CCWGG 1 cut(s) 1206
BstNSI RCATGY 2 cut(s) 371, 843
BstSCI CCNGG 1 cut(s) 1204
BstSLI GKGCMC 1 cut(s) 605
BstV1I GCAGC 4 cut(s) 106, 306, 1216, 1251
BstX2I RGATCY 1 cut(s) 1208
BstYI RGATCY 1 cut(s) 1208
Bsu36I CCTNAGG 1 cut(s) 1143
BsuRI GGCC 3 cut(s) 521, 862, 949
BtgI CCRYGG 1 cut(s) 863
BtgZI GCGATG 1 cut(s) 834
BtsCI GGATG 4 cut(s) 436, 841, 931, 1183
BtsI GCAGTG 1 cut(s) 603
BtsIMutI CAGTG 1 cut(s) 603
Cac8I GCNNGC 3 cut(s) 129, 375, 726
CciI TCATGA 1 cut(s) 76
CfoI GCGC 1 cut(s) 579
Cfr10I RCCGGY 1 cut(s) 317
Cfr13I GGNCC 3 cut(s) 188, 562, 947
Csp6I GTAC 3 cut(s) 827, 898, 1181
CspAI ACCGGT 1 cut(s) 317
CviQI GTAC 3 cut(s) 827, 898, 1181
DdeI CTNAG 4 cut(s) 355, 523, 686, 1143
DpnI GATC 4 cut(s) 510, 876, 963, 1210
DpnII GATC 4 cut(s) 508, 874, 961, 1208
Eam1104I CTCTTC 2 cut(s) 24, 1023
EarI CTCTTC 2 cut(s) 24, 1023
Eco130I CCWWGG 2 cut(s) 254, 863
Eco147I AGGCCT 1 cut(s) 521
Eco24I GRGCYC 1 cut(s) 1247
Eco47I GGWCC 2 cut(s) 188, 562
Eco81I CCTNAGG 1 cut(s) 1143
EcoO109I RGGNCCY 1 cut(s) 947
EcoRII CCWGG 1 cut(s) 1204
EcoT14I CCWWGG 2 cut(s) 254, 863
EcoT22I ATGCAT 1 cut(s) 841
EcoT38I GRGCYC 1 cut(s) 1247
ErhI CCWWGG 2 cut(s) 254, 863
FauNDI CATATG 2 cut(s) 109, 1239
FbaI TGATCA 2 cut(s) 508, 961
FblI GTMKAC 1 cut(s) 120
Fnu4HI GCNGC 4 cut(s) 95, 295, 1230, 1265
FokI GGATG 4 cut(s) 443, 848, 938, 1190
FriOI GRGCYC 1 cut(s) 1247
Fsp4HI GCNGC 4 cut(s) 95, 295, 1230, 1265
FspBI CTAG 3 cut(s) 165, 300, 306
GlaI GCGC 1 cut(s) 578
GluI GCNGC 4 cut(s) 95, 295, 1230, 1265
GsaI CCCAGC 1 cut(s) 1250
HaeIII GGCC 3 cut(s) 521, 862, 949
HapII CCGG 2 cut(s) 26, 318
HhaI GCGC 1 cut(s) 579
Hin6I GCGC 1 cut(s) 577
HinP1I GCGC 1 cut(s) 577
HincII GTYRAC 3 cut(s) 315, 403, 679
HindII GTYRAC 3 cut(s) 315, 403, 679
HindIII AAGCTT 3 cut(s) 125, 722, 882
HinfI GANTC 4 cut(s) 73, 250, 434, 1033
HpaII CCGG 2 cut(s) 26, 318
HphI GGTGA 3 cut(s) 349, 949, 1087
Hpy166II GTNNAC 5 cut(s) 121, 315, 403, 603, 679
Hpy188I TCNGA 4 cut(s) 430, 652, 966, 1132
Hpy188III TCNNGA 9 cut(s) 77, 233, 552, 700, 736, 803, 954, 989, 1259
Hpy8I GTNNAC 5 cut(s) 121, 315, 403, 603, 679
HpyAV CCTTC 6 cut(s) 61, 154, 262, 916, 960, 1012
HpyCH4III ACNGT 3 cut(s) 494, 852, 970
HpyCH4IV ACGT 1 cut(s) 766
HpyF10VI GCNNNNNNNGC 4 cut(s) 154, 416, 1261, 1270
HpyF3I CTNAG 4 cut(s) 355, 523, 686, 1143
HpySE526I ACGT 1 cut(s) 766
HspAI GCGC 1 cut(s) 577
KpnI GGTACC 1 cut(s) 830
Ksp22I TGATCA 2 cut(s) 508, 961
Kzo9I GATC 4 cut(s) 508, 874, 961, 1208
LguI GCTCTTC 1 cut(s) 24
LmnI GCTCC 1 cut(s) 272
Lsp1109I GCAGC 4 cut(s) 106, 306, 1216, 1251
LweI GCATC 2 cut(s) 826, 1264
MaeI CTAG 3 cut(s) 165, 300, 306
MaeII ACGT 1 cut(s) 766
MaeIII GTNAC 6 cut(s) 60, 85, 496, 663, 842, 1149
MalI GATC 4 cut(s) 510, 876, 963, 1210
MboI GATC 4 cut(s) 508, 874, 961, 1208
MboII GAAGA 4 cut(s) 41, 458, 628, 1040
MfeI CAATTG 1 cut(s) 149
MflI RGATCY 1 cut(s) 1208
MhlI GDGCHC 3 cut(s) 356, 605, 1247
MluCI AATT 6 cut(s) 149, 794, 1019, 1092, 1213, 1275
MlyI GAGTC 1 cut(s) 428
Mph1103I ATGCAT 1 cut(s) 841
MroXI GAANNNNTTC 2 cut(s) 344, 1032
MseI TTAA 1 cut(s) 933
MspI CCGG 2 cut(s) 26, 318
MspR9I CCNGG 1 cut(s) 1206
MunI CAATTG 1 cut(s) 149
Mva1269I GAATGC 2 cut(s) 550, 1084
MvaI CCWGG 1 cut(s) 1206
MwoI GCNNNNNNNGC 4 cut(s) 154, 416, 1261, 1270
NcoI CCATGG 1 cut(s) 863
NdeI CATATG 2 cut(s) 109, 1239
NdeII GATC 4 cut(s) 508, 874, 961, 1208
NlaIV GGNNCC 4 cut(s) 237, 274, 828, 948
NmeAIII GCCGAG 1 cut(s) 1276
NmuCI GTSAC 2 cut(s) 663, 842
NsiI ATGCAT 1 cut(s) 841
NspI RCATGY 2 cut(s) 371, 843
PagI TCATGA 1 cut(s) 76
PceI AGGCCT 1 cut(s) 521
PciSI GCTCTTC 1 cut(s) 24
PctI GAATGC 2 cut(s) 550, 1084
PdmI GAANNNNTTC 2 cut(s) 344, 1032
PfeI GAWTC 3 cut(s) 73, 250, 1033
PfoI TCCNGGA 1 cut(s) 1204
PinAI ACCGGT 1 cut(s) 317
PkrI GCNGC 4 cut(s) 96, 296, 1231, 1266
PleI GAGTC 1 cut(s) 428
PpsI GAGTC 1 cut(s) 428
Psp1406I AACGTT 1 cut(s) 766
Psp6I CCWGG 1 cut(s) 1204
PspFI CCCAGC 1 cut(s) 1246
PspGI CCWGG 1 cut(s) 1204
PspN4I GGNNCC 4 cut(s) 237, 274, 828, 948
PspPI GGNCC 3 cut(s) 188, 562, 947
PsuI RGATCY 1 cut(s) 1208
RsaI GTAC 3 cut(s) 828, 899, 1182
RsaNI GTAC 3 cut(s) 827, 898, 1181
SapI GCTCTTC 1 cut(s) 24
SaqAI TTAA 1 cut(s) 933
SatI GCNGC 4 cut(s) 95, 295, 1230, 1265
Sau3AI GATC 4 cut(s) 508, 874, 961, 1208
Sau96I GGNCC 3 cut(s) 188, 562, 947
SchI GAGTC 1 cut(s) 428
ScrFI CCNGG 1 cut(s) 1206
SduI GDGCHC 3 cut(s) 356, 605, 1247
SfaNI GCATC 2 cut(s) 826, 1264
SinI GGWCC 2 cut(s) 188, 562
SmlI CTYRAG 3 cut(s) 987, 1047, 1259
SmoI CTYRAG 3 cut(s) 987, 1047, 1259
Sse9I AATT 6 cut(s) 149, 794, 1019, 1092, 1213, 1275
SseBI AGGCCT 1 cut(s) 521
SspMI CTAG 3 cut(s) 165, 300, 306
StuI AGGCCT 1 cut(s) 521
StyD4I CCNGG 1 cut(s) 1204
StyI CCWWGG 2 cut(s) 254, 863
TaaI ACNGT 3 cut(s) 494, 852, 970
TaiI ACGT 1 cut(s) 769
TaqI TCGA 4 cut(s) 7, 699, 877, 1194
TaqII GACCGA 1 cut(s) 300
TasI AATT 6 cut(s) 149, 794, 1019, 1092, 1213, 1275
TatI WGTACW 1 cut(s) 1180
TfiI GAWTC 3 cut(s) 73, 250, 1033
Tru1I TTAA 1 cut(s) 933
Tru9I TTAA 1 cut(s) 933
TscAI CASTG 1 cut(s) 610
TseFI GTSAC 2 cut(s) 663, 842
TseI GCWGC 4 cut(s) 94, 294, 1229, 1264
Tsp45I GTSAC 2 cut(s) 663, 842
TspDTI ATGAA 8 cut(s) 36, 65, 483, 557, 581, 705, 768, 1189
TspGWI ACGGA 1 cut(s) 79
TspRI CASTG 1 cut(s) 610
VneI GTGCAC 1 cut(s) 601
VpaK11BI GGWCC 2 cut(s) 188, 562
XapI RAATTY 1 cut(s) 1019
XceI RCATGY 2 cut(s) 371, 843
XmiI GTMKAC 1 cut(s) 120
XmnI GAANNNNTTC 2 cut(s) 344, 1032
XspI CTAG 3 cut(s) 165, 300, 306
Zsp2I ATGCAT 1 cut(s) 841
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.