FvH4_4g13904

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000291
Physical Location & Seq
Reverse (-)
17429940 .. 17434769
4830 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g13904.t1

Sequence Viewer

Length: 4830 bp
ATGCCGGGAGGAAGGACAAAGCAACAAAAATTTGATTGGTATGACCAAATGCTTGGTGAACTTGCGGGAGTCCCTGATAGATTGATTGACATCACCTCCACGCTAGACCGTGTCGACCTGGATAAACTGGCGACACGAGTCAATGACCTTGGGGAATTGAGAGGGCGAGTTGCAGCCCTAGACCGCGTCGACCTGGATGAGTTGGCGGCGCGAGTCAATGACCTTGAAAGACTGAAAGGGCGAGTTACGGCCCTTGAAAAGATGAAAGCTCCAAGACTCGATGTAGAGACCGAGAGAGATGGAGGTTCTGTTTCACAAGAGCAGATGGGGGCGATGGGTGATGCCCTCGAAACTCTACAAGTGACTATGGACACCATGGCTGAGGATGTCCGAGCCACTATTGATACATTCAAGAACGAGCTGTTGGAGATGAATACCAAGCTCAATCTAGCTATCCGGGTGATGAGTAACCAACCTGTGAAGGACTACAGGAGGGTAAAGATCCCGGAACCTCAAGCTTACGGAGGGGCGCGAGACGCCAAGGAGCTTGAGAATTTTCTATTTGATATGGAGCAATACTTCCGAGCAGTGAAGTCGGATTCGGAAGAAGCAAAAGTGGGCATGGCGACAATGTATTTGTCGGGCGATGCGAAACTGTGGTGGAGAACAAAATACAACGACATCCAGAGGAATGTCTGTACCATTGCCACATGGGAGGACCTGAAGAAAGAGCTGAAGATGCAGTTCTTCCCAGAGAATGTGGACTACATTGCAAGACGACAGTTGAGAGAGCTGAAGCACACCAACAACATTAGAGAGTTTGTGAGCAAATTCTCTGTGCTCATGCTCGATCTCCCAGATATGTCGGAGAAGGACCGACTATTCTATTTCCTCGAGGGGTTAAAGCCGTGGGCTAGAACTGAACTTCAGAGACAAAGGGTTCAAGATTTGGCTTCTGCTCAAGCAGCAGCTGAAAGGTTGACAGACTACACTTTTGAAGAAAACCCTCCCAAAAGGACCTCATCCTTTTCGAATGTGAATGCCAACAAAAGTGTGAGACCAGGACAGAGTAGAAGTGGGGGAGCGGAGTCAAAGTTCTCCAGTTTCGGAGGGGGAAGGATGACCAATGTAAGGGATACAGCCTCTTCCAAACCGGCTGCATCAACAGGAGTATTTCCCCCACGACAATTCAACTCCGCCGGCTTCACACCAAGACCTATATCTTGCTTCTTATGCAAGGGGCCTCATAGGGTGAATGAGTGTCCACACAAGACGGCTTTGACAGCCCTACAAGCCCACATTTCGGCGAAGGAGTCTGAGGAACCCCAAGAGGAAGTGGAGGAACCTAGTCAGATGGGTGCTTTGAGATTCTTGAACGCAGTGGAAAAGCAACCACCCGTGTCCCAAGAGTCTCAGCCTAAGGGCCTAATGTTTGTGGATGGGAGCATAAACGGGAAGGTTGCCAAGAGTGTGATGGTCGACACTGGCGCCACCCATAATTTTGTGTCTGAAACAGAAGCACGGAAGTTGGGATTGAAGATAGAGAAGGATACTGGCCGCATGAAAGCAGTCAACTCCTCACCCTTGGCCACAGTGGGGGTGTCTCGAAGAGCACAGGTGAAGCTGGGTAGTTGGGAAGGGAAGACAGACTTGGTTGTTGTAGAGATGGACGACTTCGACGTCATATTAGGGATGGACTTTTTGCTGGAACACAAAGCCATCCCCATTCCCAATGCCCGAAATTTGTTGATCATGGGGGAGAGACCATGTGTAGTGCCTGCGAAGATAGTCCCGCCAAGTGAACCCCGTCTCTTTTCTGCTCTCCAGTTCAAGAAAGGGGTGAAGCGACATGAGCCTTCCTACGTCGCTGTCCCCTTGGTAAAAGACAGGGCTGAACCAGAAGTGGTGCCAAAAGAAGTTCAGGGGGTGCTGGAGAGTTACAAGGATGTGATGCCAGCAGATCTCCCCAAGGAGCTGCCACCGAGGAGAAACATAGATCATGAGATTGAGTTACTACCTGGTGCAAAACCACCCGCAAGGGCCCCTTACAGAATGGCTCCGCCAGAGCTAGCAGAATTGAGGAAGCAATTGGGAGATCTGTTAGAAGCAGGGTTCATTCAGCCCTCAAAGGCTCCATTTGGTGCGCCGGTGCTATTCCAAAAGAAGCATGATGGGAGCTTGAGGTTGTGTGTAGACTACCGAGCTCTTAACAAAGTCACCGTGCGGAACAAGTACCCCATTCCCTTGATTGCAGATTTGTTTGACCAGCTTAGTGGTGCCAAATATTTTACCAAGCTGGACCTCCGCTCGGGATACTACCAAGTCCGCATAACAGAAGGAGATGAGCCCAAGACTGCATGTGTCACCCGATATGGTGCTTTTGAGTTTTTGGTGATGCCGTTCGGGTTGACCAATGCACCTGCCACTTTCTGCACGTTGATGAACCAAGTCTTCCATGGTTACCTTGACAAATTTGTGGTGGTGTATTTGGATGACATTGTGATCTACAGTTCTACTCTGGAGGAACATGTGGAACACCTAAGGCTAGTGTTTCAACAGTTACGAGCCCATCAGCTGTATGTCAAGCGTGAAAAGTGCTCCTTCGCGCAAGATACTATTAAGTTCTTGGGCCATGTTATAGAGAGGGGGAGGATTAGGATGGACATGGAGAAAGTACAAGCGATCGAGGAGTGGCAGAGTCCCACGAATGTGAAGGAGCTGCGTTCTTTTCTTGGATTGGCTAATTACTATAGGCGGTTCATTAAGAATTACTCAGGAATGACGACTCCCTTGACTGACCTCTTGAAGAAGGGAGTGGTGTGGGACTGGAACGAAGACTGTGAGCAAGCCTTCATGAACTTGAAGAAGGCTGTGATGGAAGATCCTATTCTTGCCTTGCCAGACCTGAGCAAACCATTTGAAGTGCAGACCGATGCATCTGACTTCGCCCTAGGGGGAGTCCTATTACAAGAAGGGCATCCAGTGGCGTATGAGAGTCATAAGCTCTCACCAGTGGAAAGGAGATATACAGCCCATGAGAGAGAAATGTTGGCTGTGATCCACTGTTTGAGGACTTGGAGGCACTATTTGCTAGGGGCAAGTTTTACTGTGAAGACAGACAACTCTGCAGTTAGTCATTTCCTGACACAACCAAAGTTGACCTCAAAGCAAGCTCGGTGGCAAGAGTTTCTCGCCGAGTTCGACCTCCAATTTGAGCACAAAGCAGGGTACACCAATCAAGTTGTGGACGCCCTAAGCCGCAAGGCCAACCTTGCGGTCCTTCGAAACCTGGCGACTTTGTCAGGCAGTGGAGTTGCCACTGACATTAAGGAAAGAGTAAAGGCTGGTTTGGAGAAAGATCCCGTGGCTCAAGGCCTCGTAAAGCTAGTGCGAGAAGGGAAGAGCCGCAGATTCTGGTTGGAGGACGGCTTGTTAAAGACAAAAGGAGGAAGAACATATCTTCCCCGGGTGGATGGATTGCGAAGAATGTTGATGCGAGAGTGCCATGACACCTTATGGGCAGGGCATCCCGGGTGGCACAGAACCTATGCCCTCCTGAAACAGGGTTACTACTGGCCGCAGATGCGAGACTATGTGATGGAGTACACCAGGACGTGTCTCATCTGCCAACAAGACAAGGTAGAACGACAGAAGACAGCTGGGTTGCTGGAGCCGCTGGCAACACCCACTCGTCCATGGCAGAGTGTCTCCCTCGATTTCATTACCAATCTCCCGAAGGTTGGGGAAGTATCTGGAATCCTGGTCGTGGTGGACAGGTTCTCGAAGTATGCAACATTTGTTCCAACATCGAAGCATTGCTCGGCAGAAGACACAGCGAGTCTCTTCTTCAAACATGTGGTGAAGTATTGGGGAGTACCACAGAATATCGTTAGTGATCGAGACCCTAGGTTCACAGGGTCGTTTTGGACAGAGTTGTTCAAGCTACTGGGGTCTGAACTCAACATCTCCTCAAGTTATCACCCTCAGACGGATGGGCAGACTGAGAGATTCAATGCAATGTTGGAAGAATACTTGAGGCACTTTGTTCATGCAAATATGAAGAATTGGGTGGAGTTACTGGATGTGGCCCAGTTCTGTTTCAATTCGAAGAAGAGTTCAGCCACCAACAAGAGCGCGTTTGAAGTGGTGACTGGCCAGCAACCACTCTTGCCTCATACGGTGCAGGAGGTATACAAGGGGAAAAACCCGCGGGCTTTCAACTTCACCAAAGAGTGGAAAGCCAACGGGGACATTGCTCGAAGTTACTTGGAGAAAGCTGCGAGGAGAATGAAGAAGTGGGCAGATCAGAGTCGCAAACCCCGAGAGTTCCGAGAAGGAGACATGGTGCTCGTGAAGCTGCTCCCTGAACAGATCAGGAATATGCGCTCCACAGACAAGCGGTTGTTTAGGAGGTATGAAGGCCCACTTCCGGTCCTTTCGAAAGTGGGGAAGTGCTCTTACAAAGTCGACATCCTTGCCTGGATGAAAGTTCACCCTGTCTTCCATGTCAGCAACCTCAAACCTTACCAGTCTGATGCAGAAGATCAAGCCCGCAACCAGCCTACTCGACAGCATGTGGAGCTCAAGACACACAAACCAAAGGAGGTAGAAGAGATTCTGGCCGAGAGAGTAGTTGACAAGTTCGAGCAGGGGCGGGGAGACCGCCTAGTTACAGTGCGCCAGCAACAGTACTTAGTGAAGTGGAAGAAGATGGGCGAGGAAGAAATCTGTTGGGAGAATGCCGAAGCCCTGAAGAAGTTCAAGCAGAAGATTGAAGACTTCAAGCTAAGTTCAAGCCCAAGGCAGTCGTCGAGAGCGCCGACAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asp_protease_2 Chromo RVT_1 Retrotrans_gag gag-asp_proteas rve

Protein Analysis

1610

Amino Acids

183.49

Weight (kDa)

9.04

Isoelectric Point (pI)

42.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ty3_capsid PF19259 170 - 346 2.1e-11 Ty3 transposon capsid-like protein
Retrotrans_gag PF03732 208 - 303 8.9e-16 Retrotransposon gag protein
Asp_protease PF09668 476 - 574 4.2e-06 Aspartyl protease
gag-asp_proteas PF13975 479 - 568 1.8e-14 gag-polyprotein putative aspartyl protease
Asp_protease_2 PF13650 479 - 567 2e-12 Aspartyl protease
RVP_2 PF08284 488 - 573 5.2e-13 Retroviral aspartyl protease
RVT_1 PF00078 721 - 880 8.2e-39 Reverse transcriptase (RNA-dependent DNA polymerase)
RT_RNaseH_2 PF17919 943 - 1037 3.6e-34 RNase H-like domain found in reverse transcriptase
RT_RNaseH PF17917 968 - 1067 5e-33 RNase H-like domain found in reverse transcriptase
Integrase_H2C2 PF17921 1160 - 1213 5.8e-20 Integrase zinc binding domain
rve PF00665 1230 - 1328 3.5e-07 Integrase core domain
SH3_Tf2-1 PF24626 1446 - 1509 7.9e-14 Tf2-1-like, SH3 domain
Chromo PF00385 1535 - 1595 9.8e-08 Chromo (CHRromatin Organisation MOdifier) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000291)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG00860
fragaria_vesca FvH4_1g23712 FvH4_1g29809 FvH4_1g29810 FvH4_2g02661 FvH4_2g13451 FvH4_2g17921 FvH4_2g17922 FvH4_2g19251 FvH4_2g19252 FvH4_2g28221 FvH4_2g35961 FvH4_3g10372 FvH4_3g12461 FvH4_3g12461 FvH4_3g27861 FvH4_3g34591 FvH4_3g40201 FvH4_3g44612 FvH4_4g08352 FvH4_4g12251 FvH4_4g12742 FvH4_4g13904 FvH4_4g14287 FvH4_4g15243 FvH4_4g24071 FvH4_4g33448 FvH4_5g21912 FvH4_5g39371 FvH4_6g14992 FvH4_6g15221 FvH4_6g20603 FvH4_6g31093 FvH4_6g32372 FvH4_6g33012
malus_domestica MD11G1009100.v1.1 MD13G1145000.v1.1 MD16G1138700.v1.1
prunus_persica Prupe.5G246300_v2.0.a1 Prupe.7G034800_v2.0.a1 Prupe.8G084500_v2.0.a1
pyrus_communis pycom01g18240 pycom04g00330 pycom05g09010 pycom06g01670 pycom06g01690 pycom06g07180 pycom06g11620 pycom10g05720 pycom1134g00040 pycom1134g00060 pycom12486g00010 pycom12555g00230 pycom13g21670 pycom13g23130 pycom13g23140 pycom14g10580
rosa_chinensis RchiOBHm_Chr1g0330691 RchiOBHm_Chr2g0106171 RchiOBHm_Chr2g0134731 RchiOBHm_Chr2g0142791 RchiOBHm_Chr2g0149861 RchiOBHm_Chr3g0452401 RchiOBHm_Chr3g0461881 RchiOBHm_Chr4g0401341 RchiOBHm_Chr5g0075641 RchiOBHm_Chr6g0265961 RchiOBHm_Chr7g0200201 RchiOBHm_Chr7g0237811
rosa_laevigata RLG00000002773
rosa_multiflora Rmu_co8037806.1_g000001 Rmu_co8445989.1_g000001 Rmu_sc0000745.1_g000039 Rmu_sc0004448.1_g000029 Rmu_sc0004488.1_g000002 Rmu_sc0004591.1_g000018 Rmu_sc0005065.1_g000030 Rmu_sc0005150.1_g000005 Rmu_sc0005654.1_g000011 Rmu_sc0006986.1_g000016
rosa_roxburghii Rroxscaffold_164G00436490 Rroxscaffold_175G00432490 Rroxscaffold_1G00059440 Rroxscaffold_1G00064410 Rroxscaffold_2G00100310 Rroxscaffold_2G00128170 Rroxscaffold_3G00239420 Rroxscaffold_3G00264970 Rroxscaffold_3G00271550 Rroxscaffold_4G00300240 Rroxscaffold_5G00385050 Rroxscaffold_6G00399720
rosa_rugosa Rorug02G0252200
rosa_samantha Rh2DG251900 Rh7CG329900
rosa_wichuraiana Rw0G004800 Rw1G000850 Rw1G022350 Rw2G027030 Rw2G027760 Rw2G028410 Rw2G029130 Rw2G029390 Rw2G032490 Rw2G039460 Rw2G047800 Rw2G050340 Rw3G026430 Rw4G018370 Rw4G030750 Rw4G033140 Rw5G011950 Rw5G012010 Rw5G029280 Rw5G040190 Rw5G044570 Rw5G048240 Rw6G000340 Rw6G010140 Rw6G026480 Rw7G033750 Rw7G037250 Rw7G042080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2428
AasI GACNNNNNNGTC 1 cut(s) 1679
AatII GACGTC 1 cut(s) 1683
AbsI CCTCGAGG 1 cut(s) 893
Acc36I ACCTGC 1 cut(s) 2428
AccB1I GGYRCC 3 cut(s) 1487, 1906, 2276
AccB7I CCANNNNNTGG 1 cut(s) 4233
AccBSI CCGCTC 2 cut(s) 1085, 2307
AccI GTMKAC 6 cut(s) 114, 189, 1479, 2193, 4191, 4467
AccII CGCG 6 cut(s) 186, 211, 532, 2606, 4136, 4210
AclWI GGATC 4 cut(s) 496, 2876, 3052, 3353
AcoI YGGCCR 5 cut(s) 1555, 1587, 3575, 4153, 4620
AcsI RAATTY 5 cut(s) 29, 553, 830, 1741, 2471
AcuI CTGAAG 5 cut(s) 743, 755, 815, 911, 4772
AcyI GRCGYC 4 cut(s) 537, 1488, 1680, 3249
AfaI GTAC 7 cut(s) 700, 2234, 2676, 3230, 3605, 3876, 4691
AflIII ACRYGT 3 cut(s) 2527, 3614, 3853
AjiI CACGTC 1 cut(s) 3615
AjnI CCWGG 8 cut(s) 117, 192, 1060, 2017, 3288, 3608, 3759, 4478
AjuI GAANNNNNNNTTGG 4 cut(s) 407, 439, 1634, 1666
AleI CACNNNNGTG 1 cut(s) 2708
AloI GAACNNNNNNTCC 4 cut(s) 1079, 1111, 3445, 3477
Alw21I GWGCWC 8 cut(s) 843, 1615, 2206, 2600, 3219, 4350, 4457, 4584
AlwI GGATC 4 cut(s) 496, 2876, 3052, 3353
AlwNI CAGNNNCTG 2 cut(s) 971, 3414
Ama87I CYCGRG 5 cut(s) 893, 2308, 3465, 3530, 4320
ApaI GGGCCC 1 cut(s) 2044
ApeKI GCWGC 8 cut(s) 173, 965, 968, 1157, 1975, 2719, 4277, 4357
ApoI RAATTY 5 cut(s) 29, 553, 830, 1741, 2471
ArsI GACNNNNNNTTYG 6 cut(s) 619, 651, 977, 1009, 3194, 3226
Asp700I GAANNNNTTC 2 cut(s) 4614, 4757
AspA2I CCTAGG 2 cut(s) 2950, 3905
AspLEI GCGC 9 cut(s) 211, 532, 1490, 2146, 2608, 4136, 4386, 4680, 4819
AsuC2I CCSGG 7 cut(s) 6, 458, 506, 3466, 3467, 3531, 3532
AsuII TTCGAA 4 cut(s) 1031, 3283, 4106, 4439
AsuNHI GCTAGC 1 cut(s) 2068
AvaI CYCGRG 5 cut(s) 893, 2308, 3465, 3530, 4320
AvaII GGWCC 6 cut(s) 718, 874, 1017, 2299, 3277, 4432
AvrII CCTAGG 2 cut(s) 2950, 3905
AxyI CCTNAGG 2 cut(s) 1419, 2540
BaeGI GKGCMC 1 cut(s) 2044
BalI TGGCCA 2 cut(s) 1589, 4155
BanI GGYRCC 3 cut(s) 1487, 1906, 2276
BanII GRGCYC 5 cut(s) 2044, 2206, 2349, 2569, 4584
BarI GAAGNNNNNNTAC 2 cut(s) 4442, 4474
BauI CACGAG 2 cut(s) 135, 4349
BbsI GAAGAC 8 cut(s) 1649, 2443, 2841, 3119, 3659, 3834, 4492, 4782
Bbv12I GWGCWC 8 cut(s) 843, 1615, 2206, 2600, 3219, 4350, 4457, 4584
BbvCI CCTCAGC 1 cut(s) 381
BbvI GCAGC 8 cut(s) 185, 977, 980, 1144, 1962, 2706, 4264, 4344
BceAI ACGGC 5 cut(s) 264, 892, 1290, 2383, 3442
BcgI CGANNNNNNTGC 4 cut(s) 576, 610, 4457, 4491
BciT130I CCWGG 8 cut(s) 119, 194, 1062, 2019, 3290, 3610, 3761, 4480
BciVI GTATCC 3 cut(s) 1129, 1543, 2306
BclI TGATCA 1 cut(s) 1749
BcnI CCSGG 7 cut(s) 6, 458, 506, 3466, 3467, 3531, 3532
BfmI CTRYAG 4 cut(s) 487, 2506, 2749, 3126
BfoI RGCGCY 2 cut(s) 1491, 4820
BfuAI ACCTGC 1 cut(s) 2428
BfuI GTATCC 3 cut(s) 1129, 1543, 2306
BglII AGATCT 2 cut(s) 1960, 2095
BlnI CCTAGG 2 cut(s) 2950, 3905
BmcAI AGTACT 1 cut(s) 4691
Bme18I GGWCC 6 cut(s) 718, 874, 1017, 2299, 3277, 4432
BmeT110I CYCGRG 5 cut(s) 893, 2308, 3465, 3530, 4320
BmgBI CACGTC 1 cut(s) 3615
BmrI ACTGGG 2 cut(s) 3956, 4084
BmtI GCTAGC 1 cut(s) 2072
BmuI ACTGGG 2 cut(s) 3956, 4084
BoxI GACNNNNGTC 1 cut(s) 137
BpiI GAAGAC 8 cut(s) 1649, 2443, 2841, 3119, 3659, 3834, 4492, 4782
BpmI CTGGAG 5 cut(s) 1084, 1808, 1952, 2540, 3689
Bpu10I CCTNAGC 3 cut(s) 381, 2906, 3254
Bpu14I TTCGAA 4 cut(s) 1031, 3283, 4106, 4439
BpuEI CTTGAG 8 cut(s) 498, 569, 945, 2200, 3354, 3955, 4054, 4568
BpuMI CCSGG 7 cut(s) 6, 458, 506, 3466, 3467, 3531, 3532
BsaBI GATNNNNATC 1 cut(s) 89
BsaHI GRCGYC 4 cut(s) 537, 1488, 1680, 3249
BsaI GGTCTC 5 cut(s) 281, 1051, 1756, 3894, 4653
BsaWI WCCGGW 1 cut(s) 4429
BsaXI ACNNNNNCTCC 8 cut(s) 80, 110, 294, 324, 1079, 1109, 4329, 4359
Bse118I RCCGGY 3 cut(s) 1153, 1199, 2146
Bse21I CCTNAGG 2 cut(s) 1419, 2540
Bse3DI GCAATG 5 cut(s) 702, 768, 3814, 4023, 4251
Bse8I GATNNNNATC 1 cut(s) 89
BseBI CCWGG 8 cut(s) 119, 194, 1062, 2019, 3290, 3610, 3761, 4480
BseJI GATNNNNATC 1 cut(s) 89
BseMI GCAATG 5 cut(s) 702, 768, 3814, 4023, 4251
BseMII CTCAG 7 cut(s) 372, 1308, 1427, 2787, 2897, 3993, 3998
BseRI GAGGAG 5 cut(s) 1567, 1999, 2702, 3958, 4297
BseSI GKGCMC 1 cut(s) 2044
BseXI GCAGC 8 cut(s) 185, 977, 980, 1144, 1962, 2706, 4264, 4344
BseYI CCCAGC 2 cut(s) 1624, 3659
BsgI GTGCAG 3 cut(s) 2416, 2945, 4202
Bsh1236I CGCG 6 cut(s) 186, 211, 532, 2606, 4136, 4210
Bsh1285I CGRYCG 1 cut(s) 2685
BshNI GGYRCC 3 cut(s) 1487, 1906, 2276
BsiEI CGRYCG 1 cut(s) 2685
BsiHKAI GWGCWC 8 cut(s) 843, 1615, 2206, 2600, 3219, 4350, 4457, 4584
BsiHKCI CYCGRG 5 cut(s) 893, 2308, 3465, 3530, 4320
BsiSI CCGG 9 cut(s) 5, 457, 506, 1154, 1200, 2147, 3466, 3531, 4430
BslFI GGGAC 7 cut(s) 56, 1387, 1775, 1856, 2685, 2837, 4262
BsmBI CGTCTC 2 cut(s) 528, 1814
BsmFI GGGAC 7 cut(s) 56, 1387, 1775, 1856, 2685, 2837, 4262
BsmI GAATGC 2 cut(s) 1045, 4744
Bso31I GGTCTC 5 cut(s) 281, 1051, 1756, 3894, 4653
BsoBI CYCGRG 5 cut(s) 893, 2308, 3465, 3530, 4320
Bsp119I TTCGAA 4 cut(s) 1031, 3283, 4106, 4439
Bsp120I GGGCCC 1 cut(s) 2040
Bsp19I CCATGG 3 cut(s) 375, 2455, 3695
BspCNI CTCAG 7 cut(s) 373, 1309, 1426, 2786, 2898, 3994, 3997
BspFNI CGCG 6 cut(s) 186, 211, 532, 2606, 4136, 4210
BspHI TCATGA 2 cut(s) 1999, 2853
BspMAI CTGCAG 1 cut(s) 3130
BspMI ACCTGC 1 cut(s) 2428
BspOI GCTAGC 1 cut(s) 2072
BspPI GGATC 4 cut(s) 496, 2876, 3052, 3353
BspQI GCTCTTC 2 cut(s) 1603, 3395
BspT104I TTCGAA 4 cut(s) 1031, 3283, 4106, 4439
BspT107I GGYRCC 3 cut(s) 1487, 1906, 2276
BspTNI GGTCTC 5 cut(s) 281, 1051, 1756, 3894, 4653
BsrBI CCGCTC 2 cut(s) 1085, 2307
BsrDI GCAATG 5 cut(s) 702, 768, 3814, 4023, 4251
BsrFI RCCGGY 3 cut(s) 1153, 1199, 2146
BssAI RCCGGY 3 cut(s) 1153, 1199, 2146
BssNAI GTATAC 1 cut(s) 4192
BssNI GRCGYC 4 cut(s) 537, 1488, 1680, 3249
BssSI CACGAG 2 cut(s) 135, 4349
Bst1107I GTATAC 1 cut(s) 4192
Bst2BI CACGAG 2 cut(s) 135, 4349
Bst2UI CCWGG 8 cut(s) 119, 194, 1062, 2019, 3290, 3610, 3761, 4480
Bst6I CTCTTC 6 cut(s) 1150, 1603, 3395, 3848, 4106, 4605
BstACI GRCGYC 4 cut(s) 537, 1488, 1680, 3249
BstAPI GCANNNNNTGC 1 cut(s) 3088
BstBI TTCGAA 4 cut(s) 1031, 3283, 4106, 4439
BstDSI CCRYGG 6 cut(s) 375, 908, 2455, 3363, 3695, 4208
BstEII GGTNACC 1 cut(s) 2459
BstENI CCTNNNNNAGG 1 cut(s) 3560
BstFNI CGCG 6 cut(s) 186, 211, 532, 2606, 4136, 4210
BstH2I RGCGCY 2 cut(s) 1491, 4820
BstHHI GCGC 9 cut(s) 211, 532, 1490, 2146, 2608, 4136, 4386, 4680, 4819
BstMCI CGRYCG 1 cut(s) 2685
BstNI CCWGG 8 cut(s) 119, 194, 1062, 2019, 3290, 3610, 3761, 4480
BstNSI RCATGY 4 cut(s) 2361, 2531, 3857, 4577
BstPAI GACNNNNGTC 1 cut(s) 137
BstPI GGTNACC 1 cut(s) 2459
BstSFI CTRYAG 4 cut(s) 487, 2506, 2749, 3126
BstSLI GKGCMC 1 cut(s) 2044
BstUI CGCG 6 cut(s) 186, 211, 532, 2606, 4136, 4210
BstV1I GCAGC 8 cut(s) 185, 977, 980, 1144, 1962, 2706, 4264, 4344
BstV2I GAAGAC 8 cut(s) 1649, 2443, 2841, 3119, 3659, 3834, 4492, 4782
BstX2I RGATCY 5 cut(s) 501, 1960, 2095, 2881, 3358
BstXI CCANNNNNNTGG 2 cut(s) 53, 2273
BstYI RGATCY 5 cut(s) 501, 1960, 2095, 2881, 3358
BstZ17I GTATAC 1 cut(s) 4192
Bsu36I CCTNAGG 2 cut(s) 1419, 2540
BsuI GTATCC 3 cut(s) 1129, 1543, 2306
BtgI CCRYGG 6 cut(s) 375, 908, 2455, 3363, 3695, 4208
BtgZI GCGATG 2 cut(s) 347, 660
BtrI CACGTC 1 cut(s) 3615
BtsI GCAGTG 3 cut(s) 594, 1386, 3313
BveI ACCTGC 1 cut(s) 2428
CaiI CAGNNNCTG 2 cut(s) 971, 3414
CciI TCATGA 2 cut(s) 1999, 2853
CfoI GCGC 9 cut(s) 211, 532, 1490, 2146, 2608, 4136, 4386, 4680, 4819
Cfr10I RCCGGY 3 cut(s) 1153, 1199, 2146
Cfr42I CCGCGG 1 cut(s) 4211
Cfr9I CCCGGG 2 cut(s) 3465, 3530
CseI GACGC 3 cut(s) 175, 545, 3257
CsiI ACCWGGT 1 cut(s) 2017
Csp6I GTAC 7 cut(s) 699, 2233, 2675, 3229, 3604, 3875, 4690
CspCI CAANNNNNGTGG 2 cut(s) 3158, 3193
CviQI GTAC 7 cut(s) 699, 2233, 2675, 3229, 3604, 3875, 4690
DinI GGCGCC 1 cut(s) 1489
DrdI GACNNNNNNGTC 1 cut(s) 1679
DseDI GACNNNNNNGTC 1 cut(s) 1679
EaeI YGGCCR 5 cut(s) 1555, 1587, 3575, 4153, 4620
Eam1104I CTCTTC 6 cut(s) 1150, 1603, 3395, 3848, 4106, 4605
EarI CTCTTC 6 cut(s) 1150, 1603, 3395, 3848, 4106, 4605
EciI GGCGGA 2 cut(s) 1186, 2049
Ecl136II GAGCTC 2 cut(s) 2204, 4582
Eco147I AGGCCT 1 cut(s) 3375
Eco24I GRGCYC 5 cut(s) 2044, 2206, 2349, 2569, 4584
Eco31I GGTCTC 5 cut(s) 281, 1051, 1756, 3894, 4653
Eco47I GGWCC 6 cut(s) 718, 874, 1017, 2299, 3277, 4432
Eco53kI GAGCTC 2 cut(s) 2204, 4582
Eco57I CTGAAG 5 cut(s) 743, 755, 815, 911, 4772
Eco81I CCTNAGG 2 cut(s) 1419, 2540
Eco88I CYCGRG 5 cut(s) 893, 2308, 3465, 3530, 4320
Eco91I GGTNACC 1 cut(s) 2459
EcoICRI GAGCTC 2 cut(s) 2204, 4582
EcoNI CCTNNNNNAGG 1 cut(s) 3560
EcoO109I RGGNCCY 6 cut(s) 718, 1017, 1241, 1423, 2040, 2041
EcoO65I GGTNACC 1 cut(s) 2459
EcoRII CCWGG 8 cut(s) 117, 192, 1060, 2017, 3288, 3608, 3759, 4478
EcoT22I ATGCAT 1 cut(s) 2938
EcoT38I GRGCYC 5 cut(s) 2044, 2206, 2349, 2569, 4584
EgeI GGCGCC 1 cut(s) 1489
EheI GGCGCC 1 cut(s) 1489
Esp3I CGTCTC 2 cut(s) 528, 1814
FalI AAGNNNNNCTT 8 cut(s) 1634, 1666, 2029, 2061, 2585, 2617, 4410, 4442
FaqI GGGAC 7 cut(s) 56, 1387, 1775, 1856, 2685, 2837, 4262
FauI CCCGC 7 cut(s) 58, 1800, 2041, 4203, 4215, 4559, 4647
FbaI TGATCA 1 cut(s) 1749
FblI GTMKAC 6 cut(s) 114, 189, 1479, 2193, 4191, 4467
FriOI GRGCYC 5 cut(s) 2044, 2206, 2349, 2569, 4584
GlaI GCGC 9 cut(s) 210, 531, 1489, 2145, 2607, 4135, 4385, 4679, 4818
GsaI CCCAGC 2 cut(s) 1628, 3663
GsuI CTGGAG 5 cut(s) 1084, 1808, 1952, 2540, 3689
HaeII RGCGCY 2 cut(s) 1491, 4820
HapII CCGG 9 cut(s) 5, 457, 506, 1154, 1200, 2147, 3466, 3531, 4430
HgaI GACGC 3 cut(s) 175, 545, 3257
HhaI GCGC 9 cut(s) 211, 532, 1490, 2146, 2608, 4136, 4386, 4680, 4819
Hin1I GRCGYC 4 cut(s) 537, 1488, 1680, 3249
Hin6I GCGC 9 cut(s) 209, 530, 1488, 2144, 2606, 4134, 4384, 4678, 4817
HinP1I GCGC 9 cut(s) 209, 530, 1488, 2144, 2606, 4134, 4384, 4678, 4817
HincII GTYRAC 9 cut(s) 115, 190, 981, 1480, 1573, 2409, 3159, 4468, 4636
HindII GTYRAC 9 cut(s) 115, 190, 981, 1480, 1573, 2409, 3159, 4468, 4636
HindIII AAGCTT 1 cut(s) 516
HpaII CCGG 9 cut(s) 5, 457, 506, 1154, 1200, 2147, 3466, 3531, 4430
Hpy99I CGWCG 4 cut(s) 191, 1682, 1868, 4813
HpyCH4IV ACGT 4 cut(s) 1680, 1863, 2435, 3614
HpySE526I ACGT 4 cut(s) 1680, 1863, 2435, 3614
Hsp92I GRCGYC 4 cut(s) 537, 1488, 1680, 3249
HspAI GCGC 9 cut(s) 209, 530, 1488, 2144, 2606, 4134, 4384, 4678, 4817
KasI GGCGCC 1 cut(s) 1487
KroI GCCGGC 1 cut(s) 1199
KroNI GCCGGC 1 cut(s) 1201
Ksp22I TGATCA 1 cut(s) 1749
KspI CCGCGG 1 cut(s) 4211
LguI GCTCTTC 2 cut(s) 1603, 3395
Lsp1109I GCAGC 8 cut(s) 185, 977, 980, 1144, 1962, 2706, 4264, 4344
MabI ACCWGGT 1 cut(s) 2017
MaeII ACGT 4 cut(s) 1680, 1863, 2435, 3614
MbiI CCGCTC 2 cut(s) 1085, 2307
MfeI CAATTG 1 cut(s) 2087
MflI RGATCY 5 cut(s) 501, 1960, 2095, 2881, 3358
MlsI TGGCCA 2 cut(s) 1589, 4155
MluNI TGGCCA 2 cut(s) 1589, 4155
Mly113I GGCGCC 1 cut(s) 1488
MmeI TCCRAC 6 cut(s) 405, 576, 846, 3399, 3827, 4002
Mox20I TGGCCA 2 cut(s) 1589, 4155
Mph1103I ATGCAT 1 cut(s) 2938
MroNI GCCGGC 1 cut(s) 1199
MroXI GAANNNNTTC 2 cut(s) 4614, 4757
MscI TGGCCA 2 cut(s) 1589, 4155
MseI TTAA 7 cut(s) 902, 2208, 2619, 2763, 3327, 3434, 4828
MslI CAYNNNNRTG 2 cut(s) 2438, 2708
Msp20I TGGCCA 2 cut(s) 1589, 4155
MspA1I CMGCKG 5 cut(s) 971, 2575, 3659, 3676, 4210
MspI CCGG 9 cut(s) 5, 457, 506, 1154, 1200, 2147, 3466, 3531, 4430
MunI CAATTG 1 cut(s) 2087
Mva1269I GAATGC 2 cut(s) 1045, 4744
MvaI CCWGG 8 cut(s) 119, 194, 1062, 2019, 3290, 3610, 3761, 4480
MvnI CGCG 6 cut(s) 186, 211, 532, 2606, 4136, 4210
NaeI GCCGGC 1 cut(s) 1201
NarI GGCGCC 1 cut(s) 1488
NciI CCSGG 7 cut(s) 6, 458, 506, 3466, 3467, 3531, 3532
NcoI CCATGG 3 cut(s) 375, 2455, 3695
NgoMIV GCCGGC 1 cut(s) 1199
NheI GCTAGC 1 cut(s) 2068
NmeAIII GCCGAG 3 cut(s) 3220, 3800, 4648
NmuCI GTSAC 4 cut(s) 361, 2215, 2362, 4147
NsiI ATGCAT 1 cut(s) 2938
NspI RCATGY 4 cut(s) 2361, 2531, 3857, 4577
NspV TTCGAA 4 cut(s) 1031, 3283, 4106, 4439
OliI CACNNNNGTG 1 cut(s) 2708
PaeR7I CTCGAG 1 cut(s) 893
PagI TCATGA 2 cut(s) 1999, 2853
PaqCI CACCTGC 1 cut(s) 2428
PceI AGGCCT 1 cut(s) 3375
PciI ACATGT 2 cut(s) 2527, 3853
PciSI GCTCTTC 2 cut(s) 1603, 3395
PcsI WCGNNNNNNNCGW 5 cut(s) 647, 1677, 3198, 4318, 4817
PctI GAATGC 2 cut(s) 1045, 4744
PdiI GCCGGC 1 cut(s) 1201
PdmI GAANNNNTTC 2 cut(s) 4614, 4757
PfeI GAWTC 6 cut(s) 599, 1368, 3411, 3756, 4008, 4615
PflFI GACNNNGTC 3 cut(s) 110, 185, 3298
PflMI CCANNNNNTGG 1 cut(s) 4233
PfoI TCCNGGA 1 cut(s) 504
Ple19I CGATCG 1 cut(s) 2685
PluTI GGCGCC 1 cut(s) 1491
PpuMI RGGWCCY 2 cut(s) 718, 1017
PscI ACATGT 2 cut(s) 2527, 3853
PshAI GACNNNNGTC 1 cut(s) 137
Psp124BI GAGCTC 2 cut(s) 2206, 4584
Psp5II RGGWCCY 2 cut(s) 718, 1017
Psp6I CCWGG 8 cut(s) 117, 192, 1060, 2017, 3288, 3608, 3759, 4478
PspEI GGTNACC 1 cut(s) 2459
PspFI CCCAGC 2 cut(s) 1624, 3659
PspGI CCWGG 8 cut(s) 117, 192, 1060, 2017, 3288, 3608, 3759, 4478
PspOMI GGGCCC 1 cut(s) 2040
PspPPI RGGWCCY 2 cut(s) 718, 1017
PspXI VCTCGAGB 1 cut(s) 893
PstI CTGCAG 1 cut(s) 3130
PstNI CAGNNNCTG 2 cut(s) 971, 3414
PsuI RGATCY 5 cut(s) 501, 1960, 2095, 2881, 3358
PsyI GACNNNGTC 3 cut(s) 110, 185, 3298
PvuI CGATCG 1 cut(s) 2685
PvuII CAGCTG 3 cut(s) 971, 2575, 3659
RsaI GTAC 7 cut(s) 700, 2234, 2676, 3230, 3605, 3876, 4691
RsaNI GTAC 7 cut(s) 699, 2233, 2675, 3229, 3604, 3875, 4690
RseI CAYNNNNRTG 2 cut(s) 2438, 2708
SacI GAGCTC 2 cut(s) 2206, 4584
SacII CCGCGG 1 cut(s) 4211
SalI GTCGAC 4 cut(s) 113, 188, 1478, 4466
SapI GCTCTTC 2 cut(s) 1603, 3395
SaqAI TTAA 7 cut(s) 902, 2208, 2619, 2763, 3327, 3434, 4828
ScaI AGTACT 1 cut(s) 4691
SexAI ACCWGGT 1 cut(s) 2017
SfcI CTRYAG 4 cut(s) 487, 2506, 2749, 3126
SfoI GGCGCC 1 cut(s) 1489
Sfr274I CTCGAG 1 cut(s) 893
Sfr303I CCGCGG 1 cut(s) 4211
SfuI TTCGAA 4 cut(s) 1031, 3283, 4106, 4439
SgrAI CRCCGGYG 1 cut(s) 2146
SgrBI CCGCGG 1 cut(s) 4211
SinI GGWCC 6 cut(s) 718, 874, 1017, 2299, 3277, 4432
SlaI CTCGAG 1 cut(s) 893
SmaI CCCGGG 2 cut(s) 3467, 3532
SmiMI CAYNNNNRTG 2 cut(s) 2438, 2708
SmlI CTYRAG 9 cut(s) 513, 548, 893, 960, 2179, 3369, 3970, 4033, 4583
SmoI CTYRAG 9 cut(s) 513, 548, 893, 960, 2179, 3369, 3970, 4033, 4583
SseBI AGGCCT 1 cut(s) 3375
SspDI GGCGCC 1 cut(s) 1487
SspI AATATT 1 cut(s) 2285
SstI GAGCTC 2 cut(s) 2206, 4584
StuI AGGCCT 1 cut(s) 3375
TaiI ACGT 4 cut(s) 1683, 1866, 2438, 3617
TaqII GACCGA 3 cut(s) 305, 891, 2945
TatI WGTACW 3 cut(s) 2674, 3603, 4689
TauI GCSGC 6 cut(s) 209, 1560, 3261, 3408, 3580, 3676
TfiI GAWTC 6 cut(s) 599, 1368, 3411, 3756, 4008, 4615
Tru1I TTAA 7 cut(s) 902, 2208, 2619, 2763, 3327, 3434, 4828
Tru9I TTAA 7 cut(s) 902, 2208, 2619, 2763, 3327, 3434, 4828
TseFI GTSAC 4 cut(s) 361, 2215, 2362, 4147
TseI GCWGC 8 cut(s) 173, 965, 968, 1157, 1975, 2719, 4277, 4357
Tsp45I GTSAC 4 cut(s) 361, 2215, 2362, 4147
TspGWI ACGGA 3 cut(s) 537, 1537, 4004
TspMI CCCGGG 2 cut(s) 3465, 3530
Tth111I GACNNNGTC 3 cut(s) 110, 185, 3298
Van91I CCANNNNNTGG 1 cut(s) 4233
VpaK11BI GGWCC 6 cut(s) 718, 874, 1017, 2299, 3277, 4432
XagI CCTNNNNNAGG 1 cut(s) 3560
XapI RAATTY 5 cut(s) 29, 553, 830, 1741, 2471
XceI RCATGY 4 cut(s) 2361, 2531, 3857, 4577
XcmI CCANNNNNNNNNTGG 3 cut(s) 1471, 2453, 3241
XhoI CTCGAG 1 cut(s) 893
XmaI CCCGGG 2 cut(s) 3465, 3530
XmaJI CCTAGG 2 cut(s) 2950, 3905
XmiI GTMKAC 6 cut(s) 114, 189, 1479, 2193, 4191, 4467
XmnI GAANNNNTTC 2 cut(s) 4614, 4757
ZraI GACGTC 1 cut(s) 1681
ZrmI AGTACT 1 cut(s) 4691
Zsp2I ATGCAT 1 cut(s) 2938
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.