pycom04g00330
ERF Family

DNA RNA polymerases superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0000291
Physical Location & Seq
Reverse (-)
347481 .. 347930
450 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g00330.1

Sequence Viewer

Length: 450 bp
ATGGATAAGGTTCAGTTTCTTGGGTTTGTTGTGTCAAAAGACGGGATTTTTGTAGATGAATCCAAGGTTGCAGCAATTAAAGCTTGGTCACTACCACAAAATCTGCATGAATTAGTCTATTTCTATAGACGTTTTATTCATAATTTCAGTGCTATCATGGCTCTTATAACAGATTGTATGAAGACTGGAAAATTTAAATGGACTAAGGAGGCTACGGCGGCTTTTCATAATATCAAAGGGAAACTTATCAGTGCACCAGTTCTCATTTTGCCTGATTTTAGCAAACCATTTGAGTTGCATTGTGATGCATCCAAAGTGGGTATTGGGGCTGTGTTGAGCCAAGAGGGACGACCTATAGCATTTTACAATGAGAAATTGAGTGGGTCGAAAATGAATTACAGCACATATGATGTGGATTTTTATGCTGTTGTTCAAGCTCTTAAGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotrans_gag

Protein Analysis

150

Amino Acids

16.8

Weight (kDa)

9.02

Isoelectric Point (pI)

32.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RT_RNaseH_2 PF17919 67 - 149 8.7e-27 RNase H-like domain found in reverse transcriptase
RT_RNaseH PF17917 92 - 149 1.8e-16 RNase H-like domain found in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000291)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG00860
fragaria_vesca FvH4_1g23712 FvH4_1g29809 FvH4_1g29810 FvH4_2g02661 FvH4_2g13451 FvH4_2g17921 FvH4_2g17922 FvH4_2g19251 FvH4_2g19252 FvH4_2g28221 FvH4_2g35961 FvH4_3g10372 FvH4_3g12461 FvH4_3g12461 FvH4_3g27861 FvH4_3g34591 FvH4_3g40201 FvH4_3g44612 FvH4_4g08352 FvH4_4g12251 FvH4_4g12742 FvH4_4g13904 FvH4_4g14287 FvH4_4g15243 FvH4_4g24071 FvH4_4g33448 FvH4_5g21912 FvH4_5g39371 FvH4_6g14992 FvH4_6g15221 FvH4_6g20603 FvH4_6g31093 FvH4_6g32372 FvH4_6g33012
malus_domestica MD11G1009100.v1.1 MD13G1145000.v1.1 MD16G1138700.v1.1
prunus_persica Prupe.5G246300_v2.0.a1 Prupe.7G034800_v2.0.a1 Prupe.8G084500_v2.0.a1
pyrus_communis pycom01g18240 pycom04g00330 pycom05g09010 pycom06g01670 pycom06g01690 pycom06g07180 pycom06g11620 pycom10g05720 pycom1134g00040 pycom1134g00060 pycom12486g00010 pycom12555g00230 pycom13g21670 pycom13g23130 pycom13g23140 pycom14g10580
rosa_chinensis RchiOBHm_Chr1g0330691 RchiOBHm_Chr2g0106171 RchiOBHm_Chr2g0134731 RchiOBHm_Chr2g0142791 RchiOBHm_Chr2g0149861 RchiOBHm_Chr3g0452401 RchiOBHm_Chr3g0461881 RchiOBHm_Chr4g0401341 RchiOBHm_Chr5g0075641 RchiOBHm_Chr6g0265961 RchiOBHm_Chr7g0200201 RchiOBHm_Chr7g0237811
rosa_laevigata RLG00000002773
rosa_multiflora Rmu_co8037806.1_g000001 Rmu_co8445989.1_g000001 Rmu_sc0000745.1_g000039 Rmu_sc0004448.1_g000029 Rmu_sc0004488.1_g000002 Rmu_sc0004591.1_g000018 Rmu_sc0005065.1_g000030 Rmu_sc0005150.1_g000005 Rmu_sc0005654.1_g000011 Rmu_sc0006986.1_g000016
rosa_roxburghii Rroxscaffold_164G00436490 Rroxscaffold_175G00432490 Rroxscaffold_1G00059440 Rroxscaffold_1G00064410 Rroxscaffold_2G00100310 Rroxscaffold_2G00128170 Rroxscaffold_3G00239420 Rroxscaffold_3G00264970 Rroxscaffold_3G00271550 Rroxscaffold_4G00300240 Rroxscaffold_5G00385050 Rroxscaffold_6G00399720
rosa_rugosa Rorug02G0252200
rosa_samantha Rh2DG251900 Rh7CG329900
rosa_wichuraiana Rw0G004800 Rw1G000850 Rw1G022350 Rw2G027030 Rw2G027760 Rw2G028410 Rw2G029130 Rw2G029390 Rw2G032490 Rw2G039460 Rw2G047800 Rw2G050340 Rw3G026430 Rw4G018370 Rw4G030750 Rw4G033140 Rw5G011950 Rw5G012010 Rw5G029280 Rw5G040190 Rw5G044570 Rw5G048240 Rw6G000340 Rw6G010140 Rw6G026480 Rw7G033750 Rw7G037250 Rw7G042080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 167
AciI CCGC 1 cut(s) 218
AcsI RAATTY 1 cut(s) 191
AflII CTTAAG 1 cut(s) 440
AgsI TTSAA 1 cut(s) 434
AluBI AGCT 2 cut(s) 83, 437
AluI AGCT 2 cut(s) 83, 437
Alw21I GWGCWC 1 cut(s) 256
Alw44I GTGCAC 1 cut(s) 252
ApaLI GTGCAC 1 cut(s) 252
ApeKI GCWGC 1 cut(s) 71
ApoI RAATTY 1 cut(s) 191
ArsI GACNNNNNNTTYG 2 cut(s) 32, 64
BaeGI GKGCMC 1 cut(s) 256
BbsI GAAGAC 1 cut(s) 188
Bbv12I GWGCWC 1 cut(s) 256
BbvI GCAGC 1 cut(s) 83
BceAI ACGGC 1 cut(s) 231
BfmI CTRYAG 2 cut(s) 124, 354
BfrI CTTAAG 1 cut(s) 440
BisI GCNGC 2 cut(s) 72, 219
BlsI GCNGC 2 cut(s) 73, 220
BmsI GCATC 2 cut(s) 295, 317
BpiI GAAGAC 1 cut(s) 188
BsaJI CCNNGG 1 cut(s) 63
Bse1I ACTGG 2 cut(s) 190, 257
BseDI CCNNGG 1 cut(s) 63
BseGI GGATG 1 cut(s) 308
BseNI ACTGG 2 cut(s) 190, 257
BseSI GKGCMC 1 cut(s) 256
BseXI GCAGC 1 cut(s) 83
BsiHKAI GWGCWC 1 cut(s) 256
BslFI GGGAC 1 cut(s) 360
BsmFI GGGAC 1 cut(s) 360
Bsp1286I GDGCHC 1 cut(s) 256
BspACI CCGC 1 cut(s) 218
BspTI CTTAAG 1 cut(s) 440
BsrI ACTGG 2 cut(s) 190, 257
BssECI CCNNGG 1 cut(s) 63
BssT1I CCWWGG 1 cut(s) 63
BstAFI CTTAAG 1 cut(s) 440
BstDEI CTNAG 1 cut(s) 204
BstF5I GGATG 1 cut(s) 308
BstMWI GCNNNNNNNGC 3 cut(s) 80, 158, 218
BstSFI CTRYAG 2 cut(s) 124, 354
BstSLI GKGCMC 1 cut(s) 256
BstV1I GCAGC 1 cut(s) 83
BstV2I GAAGAC 1 cut(s) 188
BtsCI GGATG 1 cut(s) 308
BtsIMutI CAGTG 2 cut(s) 154, 256
CviAII CATG 2 cut(s) 107, 157
CviJI RGCY 7 cut(s) 83, 161, 212, 221, 329, 339, 437
CviKI_1 RGCY 7 cut(s) 83, 161, 212, 221, 329, 339, 437
DdeI CTNAG 1 cut(s) 204
DraI TTTAAA 1 cut(s) 196
Eco130I CCWWGG 1 cut(s) 63
EcoT14I CCWWGG 1 cut(s) 63
EcoT22I ATGCAT 1 cut(s) 310
ErhI CCWWGG 1 cut(s) 63
FaeI CATG 2 cut(s) 110, 160
FalI AAGNNNNNCTT 2 cut(s) 228, 260
FaqI GGGAC 1 cut(s) 360
FatI CATG 2 cut(s) 106, 156
FauNDI CATATG 1 cut(s) 406
Fnu4HI GCNGC 2 cut(s) 72, 219
FokI GGATG 1 cut(s) 295
Fsp4HI GCNGC 2 cut(s) 72, 219
GluI GCNGC 2 cut(s) 72, 219
Hin1II CATG 2 cut(s) 110, 160
HindIII AAGCTT 1 cut(s) 81
HinfI GANTC 1 cut(s) 59
Hpy166II GTNNAC 1 cut(s) 254
Hpy8I GTNNAC 1 cut(s) 254
HpyCH4IV ACGT 1 cut(s) 130
HpyCH4V TGCA 5 cut(s) 71, 106, 254, 298, 308
HpyF10VI GCNNNNNNNGC 3 cut(s) 80, 158, 218
HpyF3I CTNAG 1 cut(s) 204
HpySE526I ACGT 1 cut(s) 130
Hsp92II CATG 2 cut(s) 110, 160
LpnPI CCDG 3 cut(s) 171, 270, 285
Lsp1109I GCAGC 1 cut(s) 83
LweI GCATC 2 cut(s) 295, 317
MaeII ACGT 1 cut(s) 130
MaeIII GTNAC 1 cut(s) 87
MboII GAAGA 1 cut(s) 193
MhlI GDGCHC 1 cut(s) 256
MluCI AATT 6 cut(s) 75, 110, 142, 191, 374, 394
MnlI CCTC 2 cut(s) 202, 337
Mph1103I ATGCAT 1 cut(s) 310
MseI TTAA 3 cut(s) 78, 195, 441
MslI CAYNNNNRTG 1 cut(s) 303
MspCI CTTAAG 1 cut(s) 440
MwoI GCNNNNNNNGC 3 cut(s) 80, 158, 218
NdeI CATATG 1 cut(s) 406
NlaIII CATG 2 cut(s) 110, 160
NmuCI GTSAC 1 cut(s) 87
NsiI ATGCAT 1 cut(s) 310
PfeI GAWTC 1 cut(s) 59
PkrI GCNGC 2 cut(s) 73, 220
PsiI TTATAA 1 cut(s) 167
RseI CAYNNNNRTG 1 cut(s) 303
SaqAI TTAA 3 cut(s) 78, 195, 441
SatI GCNGC 2 cut(s) 72, 219
SduI GDGCHC 1 cut(s) 256
SetI ASST 6 cut(s) 12, 69, 85, 133, 355, 439
SfaNI GCATC 2 cut(s) 295, 317
SfcI CTRYAG 2 cut(s) 124, 354
SmiI ATTTAAAT 1 cut(s) 196
SmiMI CAYNNNNRTG 1 cut(s) 303
SmlI CTYRAG 1 cut(s) 440
SmoI CTYRAG 1 cut(s) 440
Sse9I AATT 6 cut(s) 75, 110, 142, 191, 374, 394
SsiI CCGC 1 cut(s) 218
StyI CCWWGG 1 cut(s) 63
SwaI ATTTAAAT 1 cut(s) 196
TaiI ACGT 1 cut(s) 133
TaqI TCGA 1 cut(s) 386
TasI AATT 6 cut(s) 75, 110, 142, 191, 374, 394
TauI GCSGC 1 cut(s) 221
TfiI GAWTC 1 cut(s) 59
Tru1I TTAA 3 cut(s) 78, 195, 441
Tru9I TTAA 3 cut(s) 78, 195, 441
TscAI CASTG 2 cut(s) 154, 256
TseFI GTSAC 1 cut(s) 87
TseI GCWGC 1 cut(s) 71
Tsp45I GTSAC 1 cut(s) 87
TspDTI ATGAA 6 cut(s) 72, 123, 128, 194, 215, 407
TspRI CASTG 2 cut(s) 154, 256
Vha464I CTTAAG 1 cut(s) 440
VneI GTGCAC 1 cut(s) 252
XapI RAATTY 1 cut(s) 191
Zsp2I ATGCAT 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.