Rw7G037250

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000291
Physical Location & Seq
Reverse (-)
56595236 .. 56599360
4125 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G037250.1

Sequence Viewer

Length: 4029 bp
ATGCGACCAGATGAATTCATTGATTGGCTTAACACCAATGAAAGAATTTTTGATTACAAGCACACTCCTGATGAAAGAAAGGTGAAGATGGTGGCGATCAAATTGACACACAAGGCCTCTGCTTGGTGGGAATCCTTGCGATCTCGCCGTGAACGCCTAGGAAAGCCGAAGATCAAGAATTGGGAAAAGATGAAGAAAGAATTGCGAAAAAGATTTTTACCAGAAAACTATACTCAAAATAACTTTATGAAACTTCACAACATAAGGCAGGGGTCGAGAACAGTTGATGAGTTCATAGAGGAGTTCGATCTCTTGACTACAAGATGTGGAGTGATAGAGGAAGAGGAGCAGTTAATTGTGGAGAAACAACTAAAAGTCCGTCAGAAGCGGCCTAGCAATGAGGACTCCTACCACAAACAAATTGCTGCCAAGTGGGTTGCACCATCTGAGACTGATAAAAACGGAGAACAAAAGATTGAATTCCAGAACAACCGCTCCAATCTCAAGGAGGGTGGCAAGCCTAAGAAGTGTTTCAAGTGTTCGGGGTTGGGACATATAGCGTCTGAATGTCCTAATCGACGGGTGGTGAACTTGGTGGAGGAAATAGGAGAAACAAGTGAAGCAAGACATGGTGAACTAATGATATCTGATGATTGTCATTATTATAATGACGAAGAAGTTACCTGGAGTGATCAAGGTGAGTCACTTATGATAAGAAGAACCATGAATGCATCAAAAATAGAAGAGCACCCAGATTGGAGAAGACATAATATATTTCATACAAAGTGCACTTCCAATGGTAAGGTGTGTCAAGTTATCATTGATGGGGGGAGTTGTGAAAACATTGTGGCACAAGAGATGGTAGACAAGTTGAACTTGCAAACAGAAAGGAAGCCAATATCCTATAAGCTGCAGTGGTTCAAAAAAGGTAGTGATGTAGTAGTCAACCAACGTTGTTTGGTATCATTCTCTATTGGAAAAAACTACCAAGACTCACAATGGTGTGATGTAGCACCAATGGATGCTTGTCATCTCTTATTAGGCCGTCCTTGGCAATTTGATAGGAGAGCTCACCATGACGGGTACCAAAACACCTACTCTTTCTTGCAGGATGGAAAGAGAATTATTTTGGGTCCATCTAGAGCAGACTACAAACCAAAATCACCCAAAGCAGAGAGCTGCAGTTTTCTCTCCTCAAAGAAGTTTTTAACTGAATCCAAAGAGAGTGGGGAATTGTATATGTTGATGGTGAAAGAAGTGGGCGCTAATGATTTTAGTTATTCCAAGCTGTTAGCACCTTTGCTGGAGGAATATAGTGAAGTCATTCCTGAGGAGTTACCTTCTGGGCTACCACCTATGCGTGACATACAACACCACATTGATCTCGTACCAGGTTCAAGTTTGCCAAACAGGCCTCACTACCGCATGAGTCCACAAGAATATGAAGAGCTCAATAGGCAAGTGACTGAGTTGCTACAGAAAGGAGTGATCCGGGAAAGTATGAGTCCGTGTGCAGTACCAGCATTACTCACACCTAAGAAGGATGGGACCTGGCGTATGTGTATTGATAGTAGAGCAATAAATAAGATAACAGTAAAGTATAGGTTTCCAATCCCACGCTTGGATGATATGCTTGACCACTTATCGGGAGCTAAGGTATTTTCAAAAATTGATTTACGAAGTGGGTACCATCAGATAAGGATAAGGCCGGGAGATGAATGGAAGACTGCTTTCAAGACCCGTGATGGTCTGTTTGAGTGGCTTGTAATGCCGTTCGGATTAACGAATGCCCCTAGCACATTCATGCGACTCATGAATCAGGTATTTCGCCCTTTTATTGGAAAATTTGTGGTTGTTTATTTCGACGATATTCTTGTGTATAGTCTTAATGAAGCAGAACACATGAAGCACCTACGAGAGATTTTTGAAGTATTGAGGGTGCAGAAGCTATATGCCAATTTGAAGAAGTGTGAGTTTTTAACTGAAAGCTTAGTTTTCTTGGGATATGTTATTTCTGCTGAAGGAATCAAAGTGGACTCGAACAAAATTGCAGCAATTTTAGAGTGGCCAACACCAAAATCCATCCATGATATTAGAAGCTTTCATGGCCTAGCTTCTTTTTATCGGCGGTTTATTCGCAATTTTAGCTCCATTATTGCTCCAATGACTGATTGTCTGCGTTCTAACGTATTCAAATGGACTGAAGAGGCTAATAAGAGTTTCCATGCAATAAAAGAAGCTATGACCAAAGCCCCTGTCTTGGCTTTACCTGATTATGATAAGGTTTTTGAGGTTGATTGTGATGCGTCCAAGAATGGTATTGGAGCTGTCTTGAGCCAAGAAGGAAAGCCCATAGCTTTCTTCAGTGAGAAATTAAATGACACCAGGCAGAGGTATTCCACATATGATGTTGAATTTTATGCCATTGTTCAATCTCTTCGGTATTGGAGGCAATATCTTATCTTCAAAGAATTTGTTCTCTACTCTGATCATGAAGCCCTCAAGTTCCTCAATGGACAACAAAAACTTAATAGGCGCCATGCCAGATGGGTCGAAGAGCTACAAGAATACAACTTTGTGATCAAGCATAAAGCTGGGGTGCAAAATAGGGTGGCTGATGCCTTGAGTAGGCGTGGAGCACTTCTATCTGTGATGGAGGTACGAGTTGACAGCTTTGATTACTTAAAGACGTTATATCAAGAAGATGTAGATTTTTGTGAGACATGGAATGAGTGCAAGGAGAAGAACTCTATTAAAGATTTCCATATTCAAGATGGTTTCTTATTCAAGAGTAGCAGGTTATGTGTCCCTAACTGTTCGCTACGGCTCTGGATCATTGAAGAAGTGCATGGAGGTGGTCTTGGTGGACATTTTGGGAAGACTAAAACTCTGTCATTAATTGAGGAGAAATTCTTTTGGCCCAGGTTGCAAAGAGATGTATTGAGGTATGTACAACGTTGTCGTATCTGCCACCTAGCTAAAAGTAGAAGTCAAAACACAGGGTTGTACACACCCCTCCCAGAACCAAAAGCTCCCTGGGAAGAAGTTAGCATGGATTTTGTGGTAGGATTACCAAGGACAAAACAGGGGTTTGATTCTATTTTTGTAGTGGTGGATAGGTTTTCCAAAATGGGACATTTTATACCTTGCAAAAAGACAATGGATGCTAGTAATATTGCCAATATCTACTTTCGTGACATTGTAAGGCTGCATGGAGTTCCTCGTACTATCACATCTGATAGAGATAGCAAGTTTGTGGGACACTTTTGGCGAACCTTGTGGAGAAAGTTGGGTACAAAGCTTCAGTTTAGTACTGCTCATCACCCTCAAACCGACGGGCAGACAGAAGTGGTGAATCGAAGCTTGGGAAACTTATTGAGAGCACTAGTTCGGAATAATAAAGCAAGTTGGGTTGAATTCCTTCCGCAAGCTGAATTTGCTTACAATAGCTCCAAGCACAGTACTACGAATATCAGTCCTTTCGAAGCCGTATACAGTAGCAACCCCAATGGGCCTTTGGATTTAATTCCATTACCTGTCACGGATCGATTCAGCGGTGATGCCAAAGATATGGCAGACCATATTAAGGAGTTACATGAACAAGTGCGGAGAAGACTTGTTGAGAGTAATCAGAAATACAAGGAGGTTGCTGACAAGCATCGTCGTTATGTTGAGTTCAAAGAGGGTGACCTGGTTTGGATCAACTTGAGCAAGGAGAGGTTTCCACGTGGGAAGTGTGGTAAACTACATGACAGAGGAGGGGGACCATACAAGATTCTCAAACGAGCTGGTAACAATGCTTATGTTCTCGAACTCCCTGATGATATTGGAGTGTCTCCTACTTTTAATGTTGCTGATTTGCAAGTCTATCATGGTGAGAATGAATCTACTGCTGTGGACTCGAGGGCGAGTCCTGTCCAACCCGGGTGCCTTTATACGGGTGTATCCTCCAACTTAGAGGAAGGAAGGCCCAAGAGGACAACTCAAGGACAACCTCCTGCTTATTTGCATGATTACATTAGGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 Retrotrans_gag rve zf-CCHC zf-H2C2

Protein Analysis

1342

Amino Acids

155.54

Weight (kDa)

8.94

Isoelectric Point (pI)

48.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotrans_gag PF03732 29 - 118 1.9e-16 Retrotransposon gag protein
RVT_1 PF00078 512 - 671 2.5e-41 Reverse transcriptase (RNA-dependent DNA polymerase)
RT_RNaseH_2 PF17919 733 - 826 6.7e-31 RNase H-like domain found in reverse transcriptase
RT_RNaseH PF17917 758 - 857 8.3e-32 RNase H-like domain found in reverse transcriptase
Integrase_H2C2 PF17921 939 - 996 9e-18 Integrase zinc binding domain
rve PF00665 1012 - 1109 3.8e-10 Integrase core domain
SH3_Tf2-1 PF24626 1229 - 1291 1.2e-17 Tf2-1-like, SH3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000291)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG00860
fragaria_vesca FvH4_1g23712 FvH4_1g29809 FvH4_1g29810 FvH4_2g02661 FvH4_2g13451 FvH4_2g17921 FvH4_2g17922 FvH4_2g19251 FvH4_2g19252 FvH4_2g28221 FvH4_2g35961 FvH4_3g10372 FvH4_3g12461 FvH4_3g12461 FvH4_3g27861 FvH4_3g34591 FvH4_3g40201 FvH4_3g44612 FvH4_4g08352 FvH4_4g12251 FvH4_4g12742 FvH4_4g13904 FvH4_4g14287 FvH4_4g15243 FvH4_4g24071 FvH4_4g33448 FvH4_5g21912 FvH4_5g39371 FvH4_6g14992 FvH4_6g15221 FvH4_6g20603 FvH4_6g31093 FvH4_6g32372 FvH4_6g33012
malus_domestica MD11G1009100.v1.1 MD13G1145000.v1.1 MD16G1138700.v1.1
prunus_persica Prupe.5G246300_v2.0.a1 Prupe.7G034800_v2.0.a1 Prupe.8G084500_v2.0.a1
pyrus_communis pycom01g18240 pycom04g00330 pycom05g09010 pycom06g01670 pycom06g01690 pycom06g07180 pycom06g11620 pycom10g05720 pycom1134g00040 pycom1134g00060 pycom12486g00010 pycom12555g00230 pycom13g21670 pycom13g23130 pycom13g23140 pycom14g10580
rosa_chinensis RchiOBHm_Chr1g0330691 RchiOBHm_Chr2g0106171 RchiOBHm_Chr2g0134731 RchiOBHm_Chr2g0142791 RchiOBHm_Chr2g0149861 RchiOBHm_Chr3g0452401 RchiOBHm_Chr3g0461881 RchiOBHm_Chr4g0401341 RchiOBHm_Chr5g0075641 RchiOBHm_Chr6g0265961 RchiOBHm_Chr7g0200201 RchiOBHm_Chr7g0237811
rosa_laevigata RLG00000002773
rosa_multiflora Rmu_co8037806.1_g000001 Rmu_co8445989.1_g000001 Rmu_sc0000745.1_g000039 Rmu_sc0004448.1_g000029 Rmu_sc0004488.1_g000002 Rmu_sc0004591.1_g000018 Rmu_sc0005065.1_g000030 Rmu_sc0005150.1_g000005 Rmu_sc0005654.1_g000011 Rmu_sc0006986.1_g000016
rosa_roxburghii Rroxscaffold_164G00436490 Rroxscaffold_175G00432490 Rroxscaffold_1G00059440 Rroxscaffold_1G00064410 Rroxscaffold_2G00100310 Rroxscaffold_2G00128170 Rroxscaffold_3G00239420 Rroxscaffold_3G00264970 Rroxscaffold_3G00271550 Rroxscaffold_4G00300240 Rroxscaffold_5G00385050 Rroxscaffold_6G00399720
rosa_rugosa Rorug02G0252200
rosa_samantha Rh2DG251900 Rh7CG329900
rosa_wichuraiana Rw0G004800 Rw1G000850 Rw1G022350 Rw2G027030 Rw2G027760 Rw2G028410 Rw2G029130 Rw2G029390 Rw2G032490 Rw2G039460 Rw2G047800 Rw2G050340 Rw3G026430 Rw4G018370 Rw4G030750 Rw4G033140 Rw5G011950 Rw5G012010 Rw5G029280 Rw5G040190 Rw5G044570 Rw5G048240 Rw6G000340 Rw6G010140 Rw6G026480 Rw7G033750 Rw7G037250 Rw7G042080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 666
Acc36I ACCTGC 1 cut(s) 2787
Acc65I GGTACC 2 cut(s) 1083, 1686
AccB1I GGYRCC 4 cut(s) 1083, 1686, 2535, 3927
AccBSI CCGCTC 1 cut(s) 495
AccI GTMKAC 2 cut(s) 864, 3492
AciI CCGC 7 cut(s) 388, 493, 1423, 2128, 3425, 3555, 3607
AclI AACGTT 2 cut(s) 952, 2956
AclWI GGATC 4 cut(s) 1483, 2840, 3552, 3707
AcoI YGGCCR 1 cut(s) 2066
AcsI RAATTY 9 cut(s) 14, 45, 479, 1844, 2414, 2471, 2909, 3416, 3434
AcuI CTGAAG 4 cut(s) 2040, 2223, 2347, 3287
AcvI CACGTG 1 cut(s) 3728
AcyI GRCGYC 1 cut(s) 2536
AfiI CCNNNNNNNGG 9 cut(s) 123, 1621, 1645, 1838, 2260, 2391, 2628, 3586, 3937
AhdI GACNNNNNGTC 1 cut(s) 2172
AhlI ACTAGT 1 cut(s) 3385
AjnI CCWGG 7 cut(s) 683, 1390, 1550, 2384, 2921, 3035, 3690
AjuI GAANNNNNNNTTGG 2 cut(s) 3347, 3379
AleI CACNNNNGTG 1 cut(s) 1000
AloI GAACNNNNNNTCC 2 cut(s) 479, 511
Alw21I GWGCWC 6 cut(s) 750, 791, 1072, 1452, 2641, 3385
Alw26I GTCTC 3 cut(s) 443, 2714, 3840
Alw44I GTGCAC 1 cut(s) 787
AlwI GGATC 4 cut(s) 1483, 2840, 3552, 3707
Ama87I CYCGRG 2 cut(s) 3901, 3923
ApaLI GTGCAC 1 cut(s) 787
ApeKI GCWGC 5 cut(s) 425, 910, 1179, 2051, 3208
ApoI RAATTY 9 cut(s) 14, 45, 479, 1844, 2414, 2471, 2909, 3416, 3434
ArsI GACNNNNNNTTYG 2 cut(s) 2241, 2273
AseI ATTAAT 1 cut(s) 2897
Asp700I GAANNNNTTC 4 cut(s) 530, 1323, 2475, 3420
Asp718I GGTACC 2 cut(s) 1083, 1686
AspA2I CCTAGG 1 cut(s) 157
AspLEI GCGC 2 cut(s) 1265, 2538
AspS9I GGNCC 6 cut(s) 1133, 1548, 2919, 3512, 3764, 3969
AsuC2I CCSGG 4 cut(s) 1493, 1710, 3924, 3925
AsuII TTCGAA 1 cut(s) 3483
AvaI CYCGRG 2 cut(s) 3901, 3923
AvaII GGWCC 3 cut(s) 1133, 1548, 3764
AvrII CCTAGG 1 cut(s) 157
AxyI CCTNAGG 1 cut(s) 1329
BaeGI GKGCMC 1 cut(s) 791
BaeI ACNNNNGTAYC 2 cut(s) 3927, 3960
BalI TGGCCA 1 cut(s) 2068
BanI GGYRCC 4 cut(s) 1083, 1686, 2535, 3927
BanII GRGCYC 2 cut(s) 1072, 1452
BbrPI CACGTG 1 cut(s) 3728
BbsI GAAGAC 4 cut(s) 769, 1730, 2885, 3619
Bbv12I GWGCWC 6 cut(s) 750, 791, 1072, 1452, 2641, 3385
BbvI GCAGC 5 cut(s) 412, 897, 1166, 2063, 3195
BceAI ACGGC 5 cut(s) 132, 1029, 1756, 2840, 3473
BciT130I CCWGG 7 cut(s) 685, 1392, 1552, 2386, 2923, 3037, 3692
BciVI GTATCC 1 cut(s) 3955
BclI TGATCA 3 cut(s) 691, 2488, 2580
BcnI CCSGG 4 cut(s) 1493, 1710, 3924, 3925
BcoDI GTCTC 3 cut(s) 443, 2714, 3840
BcuI ACTAGT 1 cut(s) 3385
BfaI CTAG 8 cut(s) 158, 393, 1140, 1794, 2111, 2975, 3168, 3386
BfmI CTRYAG 3 cut(s) 911, 1180, 1475
BfoI RGCGCY 2 cut(s) 1266, 2539
BfuAI ACCTGC 1 cut(s) 2787
BfuI GTATCC 1 cut(s) 3955
BglI GCCNNNNNGGC 1 cut(s) 1411
BisI GCNGC 6 cut(s) 389, 426, 911, 1180, 2052, 3209
BlnI CCTAGG 1 cut(s) 157
BlsI GCNGC 6 cut(s) 390, 427, 912, 1181, 2053, 3210
BmcAI AGTACT 2 cut(s) 3313, 3463
Bme18I GGWCC 3 cut(s) 1133, 1548, 3764
BmeRI GACNNNNNGTC 1 cut(s) 2172
BmeT110I CYCGRG 2 cut(s) 3901, 3923
BmgT120I GGNCC 6 cut(s) 1133, 1548, 2919, 3512, 3764, 3969
BmiI GGNNCC 7 cut(s) 1085, 1134, 1549, 1688, 2537, 3765, 3929
BmsI GCATC 7 cut(s) 740, 1012, 2293, 2608, 3154, 3550, 3667
BpiI GAAGAC 4 cut(s) 769, 1730, 2885, 3619
BplI GAGNNNNNCTC 4 cut(s) 2732, 2764, 3967, 3999
BpmI CTGGAG 2 cut(s) 706, 1325
Bpu10I CCTNAGC 1 cut(s) 1653
Bpu14I TTCGAA 1 cut(s) 3483
BpuEI CTTGAG 6 cut(s) 488, 2353, 2486, 2644, 3727, 3969
BpuMI CCSGG 4 cut(s) 1493, 1710, 3924, 3925
Bsa29I ATCGAT 1 cut(s) 3547
BsaAI YACGTR 1 cut(s) 3728
BsaHI GRCGYC 1 cut(s) 2536
BsaJI CCNNGG 7 cut(s) 157, 1049, 2921, 3035, 3036, 3074, 3923
BsaXI ACNNNNNCTCC 6 cut(s) 479, 509, 1641, 1671, 3434, 3464
Bsc4I CCNNNNNNNGG 9 cut(s) 123, 1621, 1645, 1838, 2260, 2391, 2628, 3586, 3937
Bse21I CCTNAGG 1 cut(s) 1329
Bse3DI GCAATG 1 cut(s) 403
BseBI CCWGG 7 cut(s) 685, 1392, 1552, 2386, 2923, 3037, 3692
BseCI ATCGAT 1 cut(s) 3547
BseDI CCNNGG 7 cut(s) 157, 1049, 2921, 3035, 3036, 3074, 3923
BseGI GGATG 6 cut(s) 1027, 1117, 1549, 1630, 2082, 3169
BseLI CCNNNNNNNGG 9 cut(s) 123, 1621, 1645, 1838, 2260, 2391, 2628, 3586, 3937
BseMI GCAATG 1 cut(s) 403
BseMII CTCAG 3 cut(s) 438, 1320, 1458
BseRI GAGGAG 6 cut(s) 314, 359, 1183, 1346, 2918, 3771
BseSI GKGCMC 1 cut(s) 791
BseXI GCAGC 5 cut(s) 412, 897, 1166, 2063, 3195
BseYI CCCAGC 1 cut(s) 2594
BsgI GTGCAG 2 cut(s) 1533, 1961
BshNI GGYRCC 4 cut(s) 1083, 1686, 2535, 3927
BshVI ATCGAT 1 cut(s) 3547
BsiHKAI GWGCWC 6 cut(s) 750, 791, 1072, 1452, 2641, 3385
BsiHKCI CYCGRG 2 cut(s) 3901, 3923
BsiSI CCGG 3 cut(s) 1492, 1709, 3924
BslFI GGGAC 6 cut(s) 564, 1561, 2792, 3147, 3273, 3777
BslI CCNNNNNNNGG 9 cut(s) 123, 1621, 1645, 1838, 2260, 2391, 2628, 3586, 3937
BsmAI GTCTC 3 cut(s) 443, 2714, 3840
BsmFI GGGAC 6 cut(s) 564, 1561, 2792, 3147, 3273, 3777
BsmI GAATGC 2 cut(s) 733, 1792
BsoBI CYCGRG 2 cut(s) 3901, 3923
Bsp119I TTCGAA 1 cut(s) 3483
Bsp1286I GDGCHC 6 cut(s) 750, 791, 1072, 1452, 2641, 3385
Bsp1407I TGTACA 2 cut(s) 2950, 3006
BspACI CCGC 7 cut(s) 388, 493, 1423, 2128, 3425, 3555, 3607
BspCNI CTCAG 3 cut(s) 439, 1321, 1459
BspDI ATCGAT 1 cut(s) 3547
BspHI TCATGA 2 cut(s) 1812, 2491
BspLI GGNNCC 7 cut(s) 1085, 1134, 1549, 1688, 2537, 3765, 3929
BspMAI CTGCAG 2 cut(s) 915, 1184
BspMI ACCTGC 1 cut(s) 2787
BspPI GGATC 4 cut(s) 1483, 2840, 3552, 3707
BspQI GCTCTTC 3 cut(s) 738, 1440, 2550
BspT104I TTCGAA 1 cut(s) 3483
BspT107I GGYRCC 4 cut(s) 1083, 1686, 2535, 3927
BsrBI CCGCTC 1 cut(s) 495
BsrDI GCAATG 1 cut(s) 403
BsrGI TGTACA 2 cut(s) 2950, 3006
BssECI CCNNGG 7 cut(s) 157, 1049, 2921, 3035, 3036, 3074, 3923
BssNAI GTATAC 1 cut(s) 3493
BssNI GRCGYC 1 cut(s) 2536
BssT1I CCWWGG 3 cut(s) 157, 1049, 3074
Bst1107I GTATAC 1 cut(s) 3493
Bst2UI CCWGG 7 cut(s) 685, 1392, 1552, 2386, 2923, 3037, 3692
Bst4CI ACNGT 5 cut(s) 283, 1594, 2816, 3461, 3497
Bst6I CTCTTC 6 cut(s) 336, 738, 1440, 2199, 2442, 2550
BstACI GRCGYC 1 cut(s) 2536
BstAUI TGTACA 2 cut(s) 2950, 3006
BstBAI YACGTR 1 cut(s) 3728
BstBI TTCGAA 1 cut(s) 3483
BstC8I GCNNGC 2 cut(s) 518, 3429
BstDEI CTNAG 8 cut(s) 447, 522, 1329, 1467, 1536, 1653, 1990, 3955
BstEII GGTNACC 1 cut(s) 3686
BstENI CCTNNNNNAGG 1 cut(s) 2626
BstF5I GGATG 6 cut(s) 1027, 1117, 1549, 1630, 2082, 3169
BstH2I RGCGCY 2 cut(s) 1266, 2539
BstHHI GCGC 2 cut(s) 1265, 2538
BstMAI GTCTC 3 cut(s) 443, 2714, 3840
BstMWI GCNNNNNNNGC 9 cut(s) 153, 1411, 1456, 1520, 1768, 2106, 2145, 2926, 3437
BstNI CCWGG 7 cut(s) 685, 1392, 1552, 2386, 2923, 3037, 3692
BstPI GGTNACC 1 cut(s) 3686
BstSFI CTRYAG 3 cut(s) 911, 1180, 1475
BstSLI GKGCMC 1 cut(s) 791
BstV1I GCAGC 5 cut(s) 412, 897, 1166, 2063, 3195
BstV2I GAAGAC 4 cut(s) 769, 1730, 2885, 3619
BstXI CCANNNNNNTGG 1 cut(s) 3571
BstZ17I GTATAC 1 cut(s) 3493
Bsu15I ATCGAT 1 cut(s) 3547
Bsu36I CCTNAGG 1 cut(s) 1329
BsuI GTATCC 1 cut(s) 3955
BsuTUI ATCGAT 1 cut(s) 3547
BtsCI GGATG 6 cut(s) 1027, 1117, 1549, 1630, 2082, 3169
BtsI GCAGTG 1 cut(s) 920
BtsIMutI CAGTG 2 cut(s) 920, 2371
BveI ACCTGC 1 cut(s) 2787
Cac8I GCNNGC 2 cut(s) 518, 3429
CciI TCATGA 2 cut(s) 1812, 2491
CfoI GCGC 2 cut(s) 1265, 2538
Cfr13I GGNCC 6 cut(s) 1133, 1548, 2919, 3512, 3764, 3969
Cfr9I CCCGGG 1 cut(s) 3923
ClaI ATCGAT 1 cut(s) 3547
CseI GACGC 2 cut(s) 549, 2295
CsiI ACCWGGT 2 cut(s) 1390, 3690
CspCI CAANNNNNGTGG 4 cut(s) 493, 528, 1207, 1242
DdeI CTNAG 8 cut(s) 447, 522, 1329, 1467, 1536, 1653, 1990, 3955
DinI GGCGCC 1 cut(s) 2537
DriI GACNNNNNGTC 1 cut(s) 2172
EaeI YGGCCR 1 cut(s) 2066
Eam1104I CTCTTC 6 cut(s) 336, 738, 1440, 2199, 2442, 2550
Eam1105I GACNNNNNGTC 1 cut(s) 2172
EarI CTCTTC 6 cut(s) 336, 738, 1440, 2199, 2442, 2550
Ecl136II GAGCTC 2 cut(s) 1070, 1450
Eco130I CCWWGG 3 cut(s) 157, 1049, 3074
Eco147I AGGCCT 2 cut(s) 116, 1414
Eco24I GRGCYC 2 cut(s) 1072, 1452
Eco32I GATATC 1 cut(s) 645
Eco47I GGWCC 3 cut(s) 1133, 1548, 3764
Eco53kI GAGCTC 2 cut(s) 1070, 1450
Eco57I CTGAAG 4 cut(s) 2040, 2223, 2347, 3287
Eco72I CACGTG 1 cut(s) 3728
Eco81I CCTNAGG 1 cut(s) 1329
Eco88I CYCGRG 2 cut(s) 3901, 3923
Eco91I GGTNACC 1 cut(s) 3686
EcoICRI GAGCTC 2 cut(s) 1070, 1450
EcoNI CCTNNNNNAGG 1 cut(s) 2626
EcoO109I RGGNCCY 1 cut(s) 1548
EcoO65I GGTNACC 1 cut(s) 3686
EcoRI GAATTC 3 cut(s) 14, 479, 3416
EcoRII CCWGG 7 cut(s) 683, 1390, 1550, 2384, 2921, 3035, 3690
EcoRV GATATC 1 cut(s) 645
EcoT14I CCWWGG 3 cut(s) 157, 1049, 3074
EcoT22I ATGCAT 1 cut(s) 733
EcoT38I GRGCYC 2 cut(s) 1072, 1452
EgeI GGCGCC 1 cut(s) 2537
EheI GGCGCC 1 cut(s) 2537
ErhI CCWWGG 3 cut(s) 157, 1049, 3074
FalI AAGNNNNNCTT 2 cut(s) 860, 892
FaqI GGGAC 6 cut(s) 564, 1561, 2792, 3147, 3273, 3777
FauNDI CATATG 1 cut(s) 2404
FbaI TGATCA 3 cut(s) 691, 2488, 2580
FblI GTMKAC 2 cut(s) 864, 3492
Fnu4HI GCNGC 6 cut(s) 389, 426, 911, 1180, 2052, 3209
FokI GGATG 6 cut(s) 1034, 1124, 1556, 1637, 2069, 3176
FriOI GRGCYC 2 cut(s) 1072, 1452
Fsp4HI GCNGC 6 cut(s) 389, 426, 911, 1180, 2052, 3209
FspBI CTAG 8 cut(s) 158, 393, 1140, 1794, 2111, 2975, 3168, 3386
GlaI GCGC 2 cut(s) 1264, 2537
GluI GCNGC 6 cut(s) 389, 426, 911, 1180, 2052, 3209
GsaI CCCAGC 1 cut(s) 2598
GsuI CTGGAG 2 cut(s) 706, 1325
HaeII RGCGCY 2 cut(s) 1266, 2539
HapII CCGG 3 cut(s) 1492, 1709, 3924
HgaI GACGC 2 cut(s) 549, 2295
HhaI GCGC 2 cut(s) 1265, 2538
Hin1I GRCGYC 1 cut(s) 2536
Hin6I GCGC 2 cut(s) 1263, 2536
HinP1I GCGC 2 cut(s) 1263, 2536
HincII GTYRAC 2 cut(s) 946, 2668
HindII GTYRAC 2 cut(s) 946, 2668
HindIII AAGCTT 4 cut(s) 1987, 2098, 3299, 3361
HpaII CCGG 3 cut(s) 1492, 1709, 3924
Hpy99I CGWCG 4 cut(s) 582, 1868, 3338, 3666
HpyAV CCTTC 7 cut(s) 1350, 1534, 2015, 2336, 3431, 3956, 3960
HpyCH4III ACNGT 5 cut(s) 283, 1594, 2816, 3461, 3497
HpyCH4IV ACGT 5 cut(s) 952, 2187, 2690, 2956, 3727
HpyF10VI GCNNNNNNNGC 9 cut(s) 153, 1411, 1456, 1520, 1768, 2106, 2145, 2926, 3437
HpyF3I CTNAG 8 cut(s) 447, 522, 1329, 1467, 1536, 1653, 1990, 3955
HpySE526I ACGT 5 cut(s) 952, 2187, 2690, 2956, 3727
Hsp92I GRCGYC 1 cut(s) 2536
HspAI GCGC 2 cut(s) 1263, 2536
KasI GGCGCC 1 cut(s) 2535
KpnI GGTACC 2 cut(s) 1087, 1690
Ksp22I TGATCA 3 cut(s) 691, 2488, 2580
LguI GCTCTTC 3 cut(s) 738, 1440, 2550
LmnI GCTCC 9 cut(s) 346, 500, 1649, 2153, 2164, 2324, 2636, 3037, 3455
Lsp1109I GCAGC 5 cut(s) 412, 897, 1166, 2063, 3195
LweI GCATC 7 cut(s) 740, 1012, 2293, 2608, 3154, 3550, 3667
MabI ACCWGGT 2 cut(s) 1390, 3690
MaeI CTAG 8 cut(s) 158, 393, 1140, 1794, 2111, 2975, 3168, 3386
MaeII ACGT 5 cut(s) 952, 2187, 2690, 2956, 3727
MbiI CCGCTC 1 cut(s) 495
MhlI GDGCHC 6 cut(s) 750, 791, 1072, 1452, 2641, 3385
MlsI TGGCCA 1 cut(s) 2068
MluNI TGGCCA 1 cut(s) 2068
Mly113I GGCGCC 1 cut(s) 2536
MlyI GAGTC 9 cut(s) 398, 710, 986, 1438, 1513, 1803, 2030, 3893, 3919
MmeI TCCRAC 2 cut(s) 3943, 3975
Mox20I TGGCCA 1 cut(s) 2068
Mph1103I ATGCAT 1 cut(s) 733
MroXI GAANNNNTTC 4 cut(s) 530, 1323, 2475, 3420
MscI TGGCCA 1 cut(s) 2068
MslI CAYNNNNRTG 3 cut(s) 1000, 1802, 2853
Msp20I TGGCCA 1 cut(s) 2068
MspA1I CMGCKG 1 cut(s) 3555
MspI CCGG 3 cut(s) 1492, 1709, 3924
Mva1269I GAATGC 2 cut(s) 733, 1792
MvaI CCWGG 7 cut(s) 685, 1392, 1552, 2386, 2923, 3037, 3692
MwoI GCNNNNNNNGC 9 cut(s) 153, 1411, 1456, 1520, 1768, 2106, 2145, 2926, 3437
NarI GGCGCC 1 cut(s) 2536
NciI CCSGG 4 cut(s) 1493, 1710, 3924, 3925
NdeI CATATG 1 cut(s) 2404
NlaIV GGNNCC 7 cut(s) 1085, 1134, 1549, 1688, 2537, 3765, 3929
NmuCI GTSAC 6 cut(s) 702, 1361, 1462, 3194, 3538, 3686
NsiI ATGCAT 1 cut(s) 733
NspV TTCGAA 1 cut(s) 3483
OliI CACNNNNGTG 1 cut(s) 1000
PaeR7I CTCGAG 1 cut(s) 3901
PagI TCATGA 2 cut(s) 1812, 2491
PasI CCCWGGG 1 cut(s) 3036
PceI AGGCCT 2 cut(s) 116, 1414
PciSI GCTCTTC 3 cut(s) 738, 1440, 2550
PctI GAATGC 2 cut(s) 733, 1792
PdmI GAANNNNTTC 4 cut(s) 530, 1323, 2475, 3420
PfeI GAWTC 9 cut(s) 131, 1214, 1816, 2025, 3095, 3355, 3549, 3775, 3884
PfoI TCCNGGA 1 cut(s) 1491
PkrI GCNGC 6 cut(s) 390, 427, 912, 1181, 2053, 3210
PleI GAGTC 9 cut(s) 398, 709, 986, 1437, 1512, 1803, 2030, 3893, 3918
PluTI GGCGCC 1 cut(s) 2539
PmaCI CACGTG 1 cut(s) 3728
PmlI CACGTG 1 cut(s) 3728
PpsI GAGTC 9 cut(s) 398, 709, 986, 1437, 1512, 1803, 2030, 3893, 3918
Ppu21I YACGTR 1 cut(s) 3728
PpuMI RGGWCCY 1 cut(s) 1548
PshBI ATTAAT 1 cut(s) 2897
PsiI TTATAA 1 cut(s) 666
Psp124BI GAGCTC 2 cut(s) 1072, 1452
Psp1406I AACGTT 2 cut(s) 952, 2956
Psp5II RGGWCCY 1 cut(s) 1548
Psp6I CCWGG 7 cut(s) 683, 1390, 1550, 2384, 2921, 3035, 3690
PspCI CACGTG 1 cut(s) 3728
PspEI GGTNACC 1 cut(s) 3686
PspFI CCCAGC 1 cut(s) 2594
PspGI CCWGG 7 cut(s) 683, 1390, 1550, 2384, 2921, 3035, 3690
PspN4I GGNNCC 7 cut(s) 1085, 1134, 1549, 1688, 2537, 3765, 3929
PspPI GGNCC 6 cut(s) 1133, 1548, 2919, 3512, 3764, 3969
PspPPI RGGWCCY 1 cut(s) 1548
PspXI VCTCGAGB 1 cut(s) 3901
PstI CTGCAG 2 cut(s) 915, 1184
RseI CAYNNNNRTG 3 cut(s) 1000, 1802, 2853
SacI GAGCTC 2 cut(s) 1072, 1452
SapI GCTCTTC 3 cut(s) 738, 1440, 2550
SatI GCNGC 6 cut(s) 389, 426, 911, 1180, 2052, 3209
Sau96I GGNCC 6 cut(s) 1133, 1548, 2919, 3512, 3764, 3969
ScaI AGTACT 2 cut(s) 3313, 3463
SchI GAGTC 9 cut(s) 398, 710, 986, 1438, 1513, 1803, 2030, 3893, 3919
SduI GDGCHC 6 cut(s) 750, 791, 1072, 1452, 2641, 3385
SexAI ACCWGGT 2 cut(s) 1390, 3690
SfaNI GCATC 7 cut(s) 740, 1012, 2293, 2608, 3154, 3550, 3667
SfcI CTRYAG 3 cut(s) 911, 1180, 1475
SfoI GGCGCC 1 cut(s) 2537
Sfr274I CTCGAG 1 cut(s) 3901
SfuI TTCGAA 1 cut(s) 3483
SinI GGWCC 3 cut(s) 1133, 1548, 3764
SlaI CTCGAG 1 cut(s) 3901
SmaI CCCGGG 1 cut(s) 3925
SmiMI CAYNNNNRTG 3 cut(s) 1000, 1802, 2853
SmlI CTYRAG 7 cut(s) 503, 2332, 2501, 2623, 3706, 3901, 3984
SmoI CTYRAG 7 cut(s) 503, 2332, 2501, 2623, 3706, 3901, 3984
SpeI ACTAGT 1 cut(s) 3385
SseBI AGGCCT 2 cut(s) 116, 1414
SsiI CCGC 7 cut(s) 388, 493, 1423, 2128, 3425, 3555, 3607
SspDI GGCGCC 1 cut(s) 2535
SspI AATATT 1 cut(s) 3175
SspMI CTAG 8 cut(s) 158, 393, 1140, 1794, 2111, 2975, 3168, 3386
SstI GAGCTC 2 cut(s) 1072, 1452
StuI AGGCCT 2 cut(s) 116, 1414
StyI CCWWGG 3 cut(s) 157, 1049, 3074
TaaI ACNGT 5 cut(s) 283, 1594, 2816, 3461, 3497
TaiI ACGT 5 cut(s) 955, 2190, 2693, 2959, 3730
TatI WGTACW 4 cut(s) 2950, 3006, 3311, 3461
TauI GCSGC 1 cut(s) 391
TfiI GAWTC 9 cut(s) 131, 1214, 1816, 2025, 3095, 3355, 3549, 3775, 3884
TscAI CASTG 2 cut(s) 920, 2371
TseFI GTSAC 6 cut(s) 702, 1361, 1462, 3194, 3538, 3686
TseI GCWGC 5 cut(s) 425, 910, 1179, 2051, 3208
Tsp45I GTSAC 6 cut(s) 702, 1361, 1462, 3194, 3538, 3686
TspGWI ACGGA 4 cut(s) 368, 477, 1497, 3557
TspMI CCCGGG 1 cut(s) 3923
TspRI CASTG 2 cut(s) 920, 2371
VneI GTGCAC 1 cut(s) 787
VpaK11BI GGWCC 3 cut(s) 1133, 1548, 3764
VspI ATTAAT 1 cut(s) 2897
XagI CCTNNNNNAGG 1 cut(s) 2626
XapI RAATTY 9 cut(s) 14, 45, 479, 1844, 2414, 2471, 2909, 3416, 3434
XbaI TCTAGA 1 cut(s) 1139
XcmI CCANNNNNNNNNTGG 3 cut(s) 2771, 3033, 3514
XhoI CTCGAG 1 cut(s) 3901
XmaI CCCGGG 1 cut(s) 3923
XmaJI CCTAGG 1 cut(s) 157
XmiI GTMKAC 2 cut(s) 864, 3492
XmnI GAANNNNTTC 4 cut(s) 530, 1323, 2475, 3420
XspI CTAG 8 cut(s) 158, 393, 1140, 1794, 2111, 2975, 3168, 3386
ZrmI AGTACT 2 cut(s) 3313, 3463
Zsp2I ATGCAT 1 cut(s) 733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.