FvH4_5g12490

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000255
Physical Location & Seq
Forward (+)
7046376 .. 7048600
2225 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g12490.t1

Sequence Viewer

Length: 2037 bp
ATGGCACCTTCTCTCATCGGTCCACCATCCCTCAGGCAATCCCCAACCCCGGCGGCCCAGCCACCGGCCAAAACCCCTTCCGGCGACCCATTCATCGACCTCATGGTCGCCGATTTCAACAACCTGGCCCAAAACCCTCCCAGGCCCCAAATGGGCTTCACCGAGAACGGCTCCGCCACCTTCGTCACCACCGGCGATCCTTGCCTCGACCTCTTCTTCCACGTCGTCCCCAACACTCCGGCGTCGTATCTCAACCAGCAGCTCCCTCTGGCCTGGGCCCACAACGCCCTCACCACTCTAAAACTCATCTGCAACCTCCGCGGCGTACGTGGCACCGGCAAGTCCGATAAAGAAGGGTTCTACACGGCGGCATTTTGGCTCCACAAGCACCACCCCAAAACCCTAGCCTGCAACGTGGCGTCCCTCGCCGAGTTCGGGTACTTCAAGGACCTGCCGGAGATTCTGTACCGCCTTCTTGAAGGCGAAGACGTGAGGAAGAAGCAGAAGTCCGATTGGATGATGAGGAAGTCCGGCGGGGCCAAAATTCGCAAGGCGGCGAGGGTTCCGTTTTTCAGAGGCCGCGGTGGCAGACGTGGCGGCGGTGCTAAGAGAGGCAGGGGAGGCCGCCGAGGAAACAATTACAACAAGAACCCTGTGGCGGCGAAGGAGATTCGGGTGCTGAATTCGATGGAGAGGGCAAAATGGGAAAAGGAAAAAGCCTCTGCCTTGAGGAAGGAGAAGTTCGCGGCCATGGCGAAGAAGGCGGTGGAGAGGTACCAGCGTGACCCGGACTACAGATTCTTGCACGAAAGAGTTTCGGATCTTTTTGCAGAGTGCTTGAAATTGGATATGGAGAGTTTTAAGGCCAATCAGTACAAGAAGATAAGCCTGGCTGCGAAATGGTGTCCTTCTCTGGATTCGTCTTTCGATCGTGCTACTCTGCTGTGTGAAAGCATTGCAAGGAAGGTTTTCGCTAGAGAATCGTATCCGGAGTATGAGGGAGTTGAGGATGCACATTATGCTTATAGAGTGCGAGACAGGTTGAGGAAGGATGTGTTGGTGCCGTTGAGGATAGTGCTGGAGCTGCCGGAGGTTTATATGGGGGCTAATCTGTGGGGAGCTATTCCTTACAATAGAGTGGCGTCTGTGGCGATGAAGCTTTACAAGGAGAAGTTCTTGAAGCATGATGAGGAGAGGTTTAAGAAGTATCTGGAGGATGTGAAGGCCGGGAAGAGTACTATTGCGGCCGGTGCGCTGCTTCCGCATGAGATCATAGCCTCTCTGAATGATGGGGATGGAGGGCAAGTCGCCGAGCTTCAATGGAAGAGAGTGGTGGAGGACTTGTTGAAGGAAGGGAAGATGAAGAACTGCTTGGCTGTGTGTGATGTGTCCGGAAGCATGCATGGGACTCCTATGGATGTGTGTGTGGCTTTGGGGATGTTGGTGGCTGAATTGAATGAGGAGCCCTGGAAAGGGAAGGTGATCACATTCAGTGCCAATCCTCAGCTTCATTTGATACAAGGGAATGATCTAAGCTCCAAGTCTGAGTTTGTGAAGCGAATGGATTGGGGAGGCAACACTGATTTTCAGAAGGTGTTTGATCTGATCCTCCAAGTGGCTGTGAATGGGAACTTGAAGCCGGAGCAAATGATTAAGAGGGTGTTTGTTTTCAGTGACATGGAATTCGATGAGGCTTCAGGCTATGGAGGGTATGGAGGGTATGGAATGTATGGAGGGTATGGAGGATATGGAAGGAATCAAGTTCCAAGGGGATGGGAGACTGATTATGAAGTGATTCAGAGGAAGTACAGGGAGAAAGGGTATGGTGATGCAGTGCCACAGATAGTTTTCTGGAATCTGAGGGACTCGAAGGCTACCCCAGTGCCTGGGACTCAACCAGGAGTGGCGCTACTGAGTGGCTTCTCCAAGAATTTGCTCAAGCTGTTCTTGGATAATGATGGGGAGATTCGCCCTGACACAACCATGGAAGCAGCAATTTCGGGTCCTGAGTATCAAAAGCTGGTTGTGCTGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

679

Amino Acids

75.95

Weight (kDa)

9.18

Isoelectric Point (pI)

33.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2828 PF11443 54 - 454 4.7e-136 Domain of unknown function (DUF2828)
DUF7788 PF25043 456 - 657 3.4e-78 Domain of unknown function (DUF7788)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000255)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24780 AT5G13210 AT5G43390 AT5G43400
fragaria_vesca FvH4_4g23010 FvH4_5g12462 FvH4_5g12490 FvH4_5g12491 FvH4_5g20030 FvH4_5g20031 FvH4_5g21510 FvH4_6g30070
malus_domestica MD00G1095100.v1.1 MD05G1186800.v1.1 MD05G1259500.v1.1 MD05G1260200.v1.1 MD06G1159400.v1.1 MD06G1159500.v1.1 MD06G1162500.v1.1 MD06G1164600.v1.1 MD06G1187800.v1.1 MD06G1212600.v1.1 MD06G1212700.v1.1 MD13G1126800.v1.1 MD14G1168300.v1.1 MD14G1206600.v1.1 MD14G1223900.v1.1 MD14G1230800.v1.1 MD15G1240300.v1.1 MD15G1416800.v1.1 MD16G1127300.v1.1
prunus_persica Prupe.1G218700_v2.0.a1 Prupe.4G045100_v2.0.a1 Prupe.5G159300_v2.0.a1 Prupe.5G172200_v2.0.a1 Prupe.5G172300_v2.0.a1 Prupe.5G173200_v2.0.a1 Prupe.5G173300_v2.0.a1 Prupe.5G173400_v2.0.a1 Prupe.5G219000_v2.0.a1 Prupe.5G219100_v2.0.a1 Prupe.5G219200_v2.0.a1
pyrus_communis pycom05g24390 pycom06g19020 pycom10g15060 pycom10g24800 pycom13g11010 pycom16g10800 pycom16g10810 pycom17g22350 pycom17g22360
rosa_chinensis RchiOBHm_Chr2g0136111 RchiOBHm_Chr4g0429291 RchiOBHm_Chr7g0184981 RchiOBHm_Chr7g0184991 RchiOBHm_Chr7g0185001 RchiOBHm_Chr7g0204841
rosa_laevigata RLG00000003427 RLG00000004941 RLG00000004943 RLG00000004944 RLG00000004945 RLG00000004946 RLG00000007084 RLG00000013701 RLG00000019505 RLG00000019506
rosa_multiflora Rmu_co8294559.1_g000001 Rmu_co8375195.1_g000001 Rmu_co8450783.1_g000001 Rmu_co8519567.1_g000001 Rmu_sc0000054.1_g000008 Rmu_sc0001689.1_g000017 Rmu_sc0001801.1_g000027 Rmu_sc0002070.1_g000010 Rmu_sc0002180.1_g000022 Rmu_sc0002986.1_g000018 Rmu_sc0003221.1_g000027 Rmu_sc0005629.1_g000018 Rmu_sc0009898.1_g000002 Rmu_sc0018428.1_g000003 Rmu_sc0018428.1_g000005 Rmu_sc0022816.1_g000001 Rmu_sc0041781.1_g000001 Rmu_ssc0000289.1_g000007 Rmu_ssc0000289.1_g000008 Rmu_ssc0000289.1_g000010
rosa_roxburghii Rroxscaffold_2G00108460 Rroxscaffold_3G00252720 Rroxscaffold_3G00269290 Rroxscaffold_5G00349680 Rroxscaffold_5G00370900 Rroxscaffold_6G00415020
rosa_rugosa Rorug01G0043400 Rorug02G0328200 Rorug04G0234800 Rorug06G0465600 Rorug06G0465800 Rorug06G0465900 Rorug06G0466000 Rorug06G0466100 Rorug07G0086200 Rorug07G0086200
rosa_samantha Rh2BG386700 Rh2CG365900 Rh2DG402800 Rh4AG290400 Rh4BG296100 Rh4CG311900 Rh4DG293300 Rh7AG077000 Rh7AG077100 Rh7AG077200 Rh7AG216600 Rh7BG070200 Rh7BG070300 Rh7BG070400 Rh7BG213700 Rh7CG071400 Rh7CG071600 Rh7CG071700 Rh7CG071900 Rh7CG230100 Rh7DG070800 Rh7DG070900 Rh7DG224200 Rh7DG440100
rosa_wichuraiana Rw2G030970 Rw4G025150 Rw7G005840 Rw7G005850 Rw7G005860 Rw7G018770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 104
Acc36I ACCTGC 1 cut(s) 459
Acc65I GGTACC 1 cut(s) 774
AccB1I GGYRCC 4 cut(s) 4, 332, 774, 1060
AccB7I CCANNNNNTGG 1 cut(s) 1886
AccII CGCG 3 cut(s) 321, 582, 746
AccIII TCCGGA 2 cut(s) 988, 1391
AclWI GGATC 3 cut(s) 191, 828, 1600
AcoI YGGCCR 3 cut(s) 66, 747, 1245
AcsI RAATTY 4 cut(s) 543, 682, 1682, 1930
AcuI CTGAAG 1 cut(s) 1680
AcyI GRCGYC 3 cut(s) 242, 419, 1142
AfaI GTAC 7 cut(s) 327, 440, 467, 776, 875, 1237, 1808
AfiI CCNNNNNNNGG 9 cut(s) 49, 64, 152, 435, 658, 1472, 1473, 1615, 1886
AgsI TTSAA 9 cut(s) 118, 445, 479, 841, 1180, 1319, 1348, 1456, 1636
AjiI CACGTC 3 cut(s) 223, 490, 593
AjnI CCWGG 7 cut(s) 123, 140, 272, 888, 1466, 1885, 1897
AjuI GAANNNNNNNTTGG 4 cut(s) 1040, 1072, 1355, 1387
AluBI AGCT 9 cut(s) 262, 1084, 1121, 1159, 1315, 1507, 1536, 1942, 2020
AluI AGCT 9 cut(s) 262, 1084, 1121, 1159, 1315, 1507, 1536, 1942, 2020
Alw26I GTCTC 2 cut(s) 1029, 1772
AlwI GGATC 3 cut(s) 191, 828, 1600
AlwNI CAGNNNCTG 1 cut(s) 1886
Aor13HI TCCGGA 2 cut(s) 988, 1391
ApaI GGGCCC 1 cut(s) 280
ApeKI GCWGC 5 cut(s) 259, 893, 1084, 1255, 1991
ApoI RAATTY 4 cut(s) 543, 682, 1682, 1930
ArsI GACNNNNNNTTYG 2 cut(s) 1667, 1699
Asp700I GAANNNNTTC 2 cut(s) 968, 1794
Asp718I GGTACC 1 cut(s) 774
AspLEI GCGC 2 cut(s) 1255, 1909
AspS9I GGNCC 9 cut(s) 20, 55, 127, 144, 276, 277, 448, 537, 2003
AsuC2I CCSGG 3 cut(s) 50, 788, 1228
AsuHPI GGTGA 5 cut(s) 151, 178, 283, 1492, 1838
AvaII GGWCC 3 cut(s) 20, 448, 2003
AxyI CCTNAGG 1 cut(s) 32
BaeGI GKGCMC 1 cut(s) 280
BaeI ACNNNNGTAYC 2 cut(s) 766, 799
BanI GGYRCC 4 cut(s) 4, 332, 774, 1060
BanII GRGCYC 2 cut(s) 280, 1467
BbsI GAAGAC 1 cut(s) 492
BbvCI CCTCAGC 1 cut(s) 1503
BbvI GCAGC 5 cut(s) 271, 880, 1071, 1242, 2003
BccI CCATC 6 cut(s) 34, 682, 1283, 1289, 1767, 1952
BceAI ACGGC 3 cut(s) 184, 381, 1048
BcgI CGANNNNNNTGC 2 cut(s) 1980, 2014
BciT130I CCWGG 7 cut(s) 125, 142, 274, 890, 1468, 1887, 1899
BciVI GTATCC 1 cut(s) 996
BclI TGATCA 1 cut(s) 1482
BcnI CCSGG 3 cut(s) 50, 788, 1228
BcoDI GTCTC 2 cut(s) 1029, 1772
BfaI CTAG 2 cut(s) 404, 975
BfmI CTRYAG 1 cut(s) 793
BfoI RGCGCY 1 cut(s) 1910
BfuAI ACCTGC 1 cut(s) 459
BfuI GTATCC 1 cut(s) 996
BglI GCCNNNNNGGC 1 cut(s) 585
BmcAI AGTACT 1 cut(s) 1237
Bme18I GGWCC 3 cut(s) 20, 448, 2003
BmgBI CACGTC 3 cut(s) 223, 490, 593
BmgT120I GGNCC 9 cut(s) 20, 55, 127, 144, 276, 277, 448, 537, 2003
BmrI ACTGGG 1 cut(s) 1874
BmsI GCATC 2 cut(s) 1000, 1819
BmuI ACTGGG 1 cut(s) 1874
BpiI GAAGAC 1 cut(s) 492
BplI GAGNNNNNCTC 2 cut(s) 155, 187
BpmI CTGGAG 2 cut(s) 1100, 1232
Bpu10I CCTNAGC 1 cut(s) 1503
BpuEI CTTGAG 2 cut(s) 748, 1922
BpuMI CCSGG 3 cut(s) 50, 788, 1228
BsaAI YACGTR 1 cut(s) 329
BsaHI GRCGYC 3 cut(s) 242, 419, 1142
BsaWI WCCGGW 2 cut(s) 988, 1391
BsaXI ACNNNNNCTCC 8 cut(s) 449, 479, 1290, 1320, 1328, 1358, 1805, 1835
Bsc4I CCNNNNNNNGG 9 cut(s) 49, 64, 152, 435, 658, 1472, 1473, 1615, 1886
Bse118I RCCGGY 4 cut(s) 64, 191, 335, 1247
Bse1I ACTGG 1 cut(s) 1880
Bse21I CCTNAGG 1 cut(s) 32
Bse3DI GCAATG 1 cut(s) 954
BseAI TCCGGA 2 cut(s) 988, 1391
BseBI CCWGG 7 cut(s) 125, 142, 274, 890, 1468, 1887, 1899
BseGI GGATG 9 cut(s) 26, 522, 1015, 1057, 1222, 1300, 1423, 1443, 1778
BseLI CCNNNNNNNGG 9 cut(s) 49, 64, 152, 435, 658, 1472, 1473, 1615, 1886
BseMI GCAATG 1 cut(s) 954
BseMII CTCAG 6 cut(s) 46, 1517, 1536, 1850, 1904, 1998
BseNI ACTGG 1 cut(s) 1880
BseRI GAGGAG 2 cut(s) 1205, 1475
BseSI GKGCMC 1 cut(s) 280
BseX3I CGGCCG 1 cut(s) 1245
BseXI GCAGC 5 cut(s) 271, 880, 1071, 1242, 2003
BseYI CCCAGC 1 cut(s) 57
Bsh1236I CGCG 3 cut(s) 321, 582, 746
Bsh1285I CGRYCG 2 cut(s) 931, 1248
BshNI GGYRCC 4 cut(s) 4, 332, 774, 1060
BsiEI CGRYCG 2 cut(s) 931, 1248
BsiWI CGTACG 1 cut(s) 325
BslFI GGGAC 5 cut(s) 212, 406, 1420, 1877, 1903
BslI CCNNNNNNNGG 9 cut(s) 49, 64, 152, 435, 658, 1472, 1473, 1615, 1886
BsmAI GTCTC 2 cut(s) 1029, 1772
BsmFI GGGAC 5 cut(s) 212, 406, 1420, 1877, 1903
Bsp120I GGGCCC 1 cut(s) 276
Bsp1286I GDGCHC 2 cut(s) 280, 1467
Bsp13I TCCGGA 2 cut(s) 988, 1391
Bsp143I GATC 8 cut(s) 196, 820, 928, 1269, 1482, 1528, 1600, 1605
Bsp19I CCATGG 2 cut(s) 750, 1983
BspCNI CTCAG 6 cut(s) 45, 1516, 1537, 1851, 1905, 1999
BspEI TCCGGA 2 cut(s) 988, 1391
BspFNI CGCG 3 cut(s) 321, 582, 746
BspMI ACCTGC 1 cut(s) 459
BspPI GGATC 3 cut(s) 191, 828, 1600
BspT107I GGYRCC 4 cut(s) 4, 332, 774, 1060
BsrDI GCAATG 1 cut(s) 954
BsrFI RCCGGY 4 cut(s) 64, 191, 335, 1247
BsrI ACTGG 1 cut(s) 1880
BssAI RCCGGY 4 cut(s) 64, 191, 335, 1247
BssMI GATC 8 cut(s) 196, 820, 928, 1269, 1482, 1528, 1600, 1605
BssNI GRCGYC 3 cut(s) 242, 419, 1142
BssT1I CCWWGG 3 cut(s) 750, 1766, 1983
Bst2UI CCWGG 7 cut(s) 125, 142, 274, 890, 1468, 1887, 1899
Bst6I CTCTTC 3 cut(s) 218, 1226, 1319
BstACI GRCGYC 3 cut(s) 242, 419, 1142
BstAPI GCANNNNNTGC 1 cut(s) 1019
BstBAI YACGTR 1 cut(s) 329
BstC8I GCNNGC 2 cut(s) 409, 1400
BstDEI CTNAG 8 cut(s) 32, 606, 1503, 1532, 1545, 1859, 1913, 2007
BstDSI CCRYGG 4 cut(s) 319, 580, 750, 1983
BstF5I GGATG 9 cut(s) 26, 522, 1015, 1057, 1222, 1300, 1423, 1443, 1778
BstFNI CGCG 3 cut(s) 321, 582, 746
BstH2I RGCGCY 1 cut(s) 1910
BstHHI GCGC 2 cut(s) 1255, 1909
BstKTI GATC 8 cut(s) 199, 823, 931, 1272, 1485, 1531, 1603, 1608
BstMAI GTCTC 2 cut(s) 1029, 1772
BstMBI GATC 8 cut(s) 196, 820, 928, 1269, 1482, 1528, 1600, 1605
BstMCI CGRYCG 2 cut(s) 931, 1248
BstNI CCWGG 7 cut(s) 125, 142, 274, 890, 1468, 1887, 1899
BstNSI RCATGY 1 cut(s) 1402
BstSFI CTRYAG 1 cut(s) 793
BstSLI GKGCMC 1 cut(s) 280
BstUI CGCG 3 cut(s) 321, 582, 746
BstV1I GCAGC 5 cut(s) 271, 880, 1071, 1242, 2003
BstV2I GAAGAC 1 cut(s) 492
BstX2I RGATCY 1 cut(s) 820
BstXI CCANNNNNNTGG 1 cut(s) 1773
BstYI RGATCY 1 cut(s) 820
BstZI CGGCCG 1 cut(s) 1245
Bsu36I CCTNAGG 1 cut(s) 32
BsuI GTATCC 1 cut(s) 996
BtgI CCRYGG 4 cut(s) 319, 580, 750, 1983
BtgZI GCGATG 1 cut(s) 1166
BtrI CACGTC 3 cut(s) 223, 490, 593
BtsCI GGATG 9 cut(s) 26, 522, 1015, 1057, 1222, 1300, 1423, 1443, 1778
BtsI GCAGTG 1 cut(s) 1839
BtsIMutI CAGTG 5 cut(s) 1498, 1578, 1678, 1839, 1887
BveI ACCTGC 1 cut(s) 459
Cac8I GCNNGC 2 cut(s) 409, 1400
CaiI CAGNNNCTG 1 cut(s) 1886
CfoI GCGC 2 cut(s) 1255, 1909
Cfr10I RCCGGY 4 cut(s) 64, 191, 335, 1247
Cfr13I GGNCC 9 cut(s) 20, 55, 127, 144, 276, 277, 448, 537, 2003
Cfr42I CCGCGG 2 cut(s) 322, 583
CseI GACGC 3 cut(s) 231, 408, 1131
Csp6I GTAC 7 cut(s) 326, 439, 466, 775, 874, 1236, 1807
CviAII CATG 8 cut(s) 103, 751, 1184, 1265, 1399, 1403, 1678, 1984
CviQI GTAC 7 cut(s) 326, 439, 466, 775, 874, 1236, 1807
DdeI CTNAG 8 cut(s) 32, 606, 1503, 1532, 1545, 1859, 1913, 2007
DpnI GATC 8 cut(s) 198, 822, 930, 1271, 1484, 1530, 1602, 1607
DpnII GATC 8 cut(s) 196, 820, 928, 1269, 1482, 1528, 1600, 1605
DrdI GACNNNNNNGTC 1 cut(s) 104
DseDI GACNNNNNNGTC 1 cut(s) 104
EaeI YGGCCR 3 cut(s) 66, 747, 1245
EagI CGGCCG 1 cut(s) 1245
Eam1104I CTCTTC 3 cut(s) 218, 1226, 1319
EarI CTCTTC 3 cut(s) 218, 1226, 1319
EciI GGCGGA 1 cut(s) 163
EclXI CGGCCG 1 cut(s) 1245
Eco130I CCWWGG 3 cut(s) 750, 1766, 1983
Eco24I GRGCYC 2 cut(s) 280, 1467
Eco47I GGWCC 3 cut(s) 20, 448, 2003
Eco52I CGGCCG 1 cut(s) 1245
Eco57I CTGAAG 1 cut(s) 1680
Eco81I CCTNAGG 1 cut(s) 32
EcoO109I RGGNCCY 3 cut(s) 144, 448, 2003
EcoRI GAATTC 2 cut(s) 682, 1682
EcoRII CCWGG 7 cut(s) 123, 140, 272, 888, 1466, 1885, 1897
EcoT14I CCWWGG 3 cut(s) 750, 1766, 1983
EcoT22I ATGCAT 1 cut(s) 1404
EcoT38I GRGCYC 2 cut(s) 280, 1467
ErhI CCWWGG 3 cut(s) 750, 1766, 1983
FaeI CATG 8 cut(s) 106, 754, 1187, 1268, 1402, 1406, 1681, 1987
FalI AAGNNNNNCTT 4 cut(s) 1355, 1387, 1931, 1963
FaqI GGGAC 5 cut(s) 212, 406, 1420, 1877, 1903
FatI CATG 8 cut(s) 102, 750, 1183, 1264, 1398, 1402, 1677, 1983
FauI CCCGC 1 cut(s) 527
FbaI TGATCA 1 cut(s) 1482
FokI GGATG 9 cut(s) 13, 529, 1022, 1064, 1229, 1307, 1430, 1450, 1785
FriOI GRGCYC 2 cut(s) 280, 1467
FspBI CTAG 2 cut(s) 404, 975
GlaI GCGC 2 cut(s) 1254, 1908
GsaI CCCAGC 1 cut(s) 61
GsuI CTGGAG 2 cut(s) 1100, 1232
HaeII RGCGCY 1 cut(s) 1910
HgaI GACGC 3 cut(s) 231, 408, 1131
HhaI GCGC 2 cut(s) 1255, 1909
Hin1I GRCGYC 3 cut(s) 242, 419, 1142
Hin1II CATG 8 cut(s) 106, 754, 1187, 1268, 1402, 1406, 1681, 1987
Hin6I GCGC 2 cut(s) 1253, 1907
HinP1I GCGC 2 cut(s) 1253, 1907
HindIII AAGCTT 1 cut(s) 1157
HphI GGTGA 5 cut(s) 151, 178, 283, 1492, 1838
Hpy166II GTNNAC 1 cut(s) 23
Hpy188III TCNNGA 8 cut(s) 476, 914, 989, 1177, 1211, 1392, 1852, 2006
Hpy8I GTNNAC 1 cut(s) 23
Hpy99I CGWCG 2 cut(s) 227, 247
HpyCH4IV ACGT 5 cut(s) 222, 328, 414, 489, 592
HpyCH4V TGCA 8 cut(s) 312, 411, 805, 830, 959, 1013, 1402, 1832
HpyF3I CTNAG 8 cut(s) 32, 606, 1503, 1532, 1545, 1859, 1913, 2007
HpySE526I ACGT 5 cut(s) 222, 328, 414, 489, 592
Hsp92I GRCGYC 3 cut(s) 242, 419, 1142
Hsp92II CATG 8 cut(s) 106, 754, 1187, 1268, 1402, 1406, 1681, 1987
HspAI GCGC 2 cut(s) 1253, 1907
Kpn2I TCCGGA 2 cut(s) 988, 1391
KpnI GGTACC 1 cut(s) 778
Ksp22I TGATCA 1 cut(s) 1482
KspI CCGCGG 2 cut(s) 322, 583
Kzo9I GATC 8 cut(s) 196, 820, 928, 1269, 1482, 1528, 1600, 1605
LmnI GCTCC 8 cut(s) 176, 267, 384, 1081, 1118, 1462, 1541, 1642
Lsp1109I GCAGC 5 cut(s) 271, 880, 1071, 1242, 2003
LweI GCATC 2 cut(s) 1000, 1819
MaeI CTAG 2 cut(s) 404, 975
MaeII ACGT 5 cut(s) 222, 328, 414, 489, 592
MaeIII GTNAC 3 cut(s) 184, 782, 1673
MalI GATC 8 cut(s) 198, 822, 930, 1271, 1484, 1530, 1602, 1607
MboI GATC 8 cut(s) 196, 820, 928, 1269, 1482, 1528, 1600, 1605
MflI RGATCY 1 cut(s) 820
MhlI GDGCHC 2 cut(s) 280, 1467
MluCI AATT 8 cut(s) 543, 637, 682, 842, 1451, 1682, 1930, 1995
MlyI GAGTC 3 cut(s) 1402, 1859, 1885
Mph1103I ATGCAT 1 cut(s) 1404
MroI TCCGGA 2 cut(s) 988, 1391
MroXI GAANNNNTTC 2 cut(s) 968, 1794
MseI TTAA 3 cut(s) 861, 1200, 1653
MslI CAYNNNNRTG 1 cut(s) 1982
MspA1I CMGCKG 2 cut(s) 321, 582
MvaI CCWGG 7 cut(s) 125, 142, 274, 890, 1468, 1887, 1899
MvnI CGCG 3 cut(s) 321, 582, 746
NciI CCSGG 3 cut(s) 50, 788, 1228
NcoI CCATGG 2 cut(s) 750, 1983
NdeII GATC 8 cut(s) 196, 820, 928, 1269, 1482, 1528, 1600, 1605
NlaIII CATG 8 cut(s) 106, 754, 1187, 1268, 1402, 1406, 1681, 1987
NmeAIII GCCGAG 3 cut(s) 454, 653, 1336
NmuCI GTSAC 3 cut(s) 184, 782, 1673
NsiI ATGCAT 1 cut(s) 1404
NspI RCATGY 1 cut(s) 1402
PaeI GCATGC 1 cut(s) 1402
PdmI GAANNNNTTC 2 cut(s) 968, 1794
PfeI GAWTC 9 cut(s) 460, 670, 798, 917, 980, 1756, 1795, 1855, 1966
Pfl23II CGTACG 1 cut(s) 325
PflMI CCANNNNNTGG 1 cut(s) 1886
Ple19I CGATCG 1 cut(s) 931
PleI GAGTC 3 cut(s) 1402, 1859, 1885
PpsI GAGTC 3 cut(s) 1402, 1859, 1885
Ppu21I YACGTR 1 cut(s) 329
PpuMI RGGWCCY 2 cut(s) 448, 2003
Psp5II RGGWCCY 2 cut(s) 448, 2003
Psp6I CCWGG 7 cut(s) 123, 140, 272, 888, 1466, 1885, 1897
PspFI CCCAGC 1 cut(s) 57
PspGI CCWGG 7 cut(s) 123, 140, 272, 888, 1466, 1885, 1897
PspLI CGTACG 1 cut(s) 325
PspOMI GGGCCC 1 cut(s) 276
PspPI GGNCC 9 cut(s) 20, 55, 127, 144, 276, 277, 448, 537, 2003
PspPPI RGGWCCY 2 cut(s) 448, 2003
PstNI CAGNNNCTG 1 cut(s) 1886
PsuI RGATCY 1 cut(s) 820
PvuI CGATCG 1 cut(s) 931
RsaI GTAC 7 cut(s) 327, 440, 467, 776, 875, 1237, 1808
RsaNI GTAC 7 cut(s) 326, 439, 466, 775, 874, 1236, 1807
RseI CAYNNNNRTG 1 cut(s) 1982
SacII CCGCGG 2 cut(s) 322, 583
SaqAI TTAA 3 cut(s) 861, 1200, 1653
Sau3AI GATC 8 cut(s) 196, 820, 928, 1269, 1482, 1528, 1600, 1605
Sau96I GGNCC 9 cut(s) 20, 55, 127, 144, 276, 277, 448, 537, 2003
ScaI AGTACT 1 cut(s) 1237
SchI GAGTC 3 cut(s) 1402, 1859, 1885
SduI GDGCHC 2 cut(s) 280, 1467
SfaNI GCATC 2 cut(s) 1000, 1819
SfcI CTRYAG 1 cut(s) 793
Sfr303I CCGCGG 2 cut(s) 322, 583
SgrAI CRCCGGYG 1 cut(s) 191
SgrBI CCGCGG 2 cut(s) 322, 583
SinI GGWCC 3 cut(s) 20, 448, 2003
SmiMI CAYNNNNRTG 1 cut(s) 1982
SmlI CTYRAG 2 cut(s) 727, 1937
SmoI CTYRAG 2 cut(s) 727, 1937
SphI GCATGC 1 cut(s) 1402
Sse9I AATT 8 cut(s) 543, 637, 682, 842, 1451, 1682, 1930, 1995
SspMI CTAG 2 cut(s) 404, 975
StyI CCWWGG 3 cut(s) 750, 1766, 1983
TaiI ACGT 5 cut(s) 225, 331, 417, 492, 595
TaqI TCGA 6 cut(s) 96, 207, 686, 927, 1686, 1868
TaqII GACCGA 1 cut(s) 8
TasI AATT 8 cut(s) 543, 637, 682, 842, 1451, 1682, 1930, 1995
TatI WGTACW 3 cut(s) 873, 1235, 1806
TfiI GAWTC 9 cut(s) 460, 670, 798, 917, 980, 1756, 1795, 1855, 1966
Tru1I TTAA 3 cut(s) 861, 1200, 1653
Tru9I TTAA 3 cut(s) 861, 1200, 1653
TscAI CASTG 5 cut(s) 1498, 1585, 1678, 1839, 1887
TseFI GTSAC 3 cut(s) 184, 782, 1673
TseI GCWGC 5 cut(s) 259, 893, 1084, 1255, 1991
Tsp45I GTSAC 3 cut(s) 184, 782, 1673
TspDTI ATGAA 5 cut(s) 82, 1169, 1376, 1499, 1803
TspGWI ACGGA 1 cut(s) 555
TspRI CASTG 5 cut(s) 1498, 1585, 1678, 1839, 1887
Van91I CCANNNNNTGG 1 cut(s) 1886
VpaK11BI GGWCC 3 cut(s) 20, 448, 2003
XapI RAATTY 4 cut(s) 543, 682, 1682, 1930
XceI RCATGY 1 cut(s) 1402
XcmI CCANNNNNNNNNTGG 1 cut(s) 148
XmnI GAANNNNTTC 2 cut(s) 968, 1794
XspI CTAG 2 cut(s) 404, 975
ZrmI AGTACT 1 cut(s) 1237
Zsp2I ATGCAT 1 cut(s) 1404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.