MD06G1212600.v1.1

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0000255
Physical Location & Seq
Reverse (-)
34523839 .. 34525884
2046 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1212600.v1.1.491

Sequence Viewer

Length: 2046 bp
ATGGCTCCTCCAAGTCTCCTCGGTCCTCCGGAGCTCAGGACCCCGACCCCACCACCCCAACCCCAACCCGCAGCCCCCTCTGGCAACCCATTCGTCGACCTCATGGTCGCAAATTACAACGACGCAGCCAAGGTTCCGGTGATCGCACCTCCGATGGGATACACCGAGAACGGCGCCTCCACGTTCCTCAACTCCGGCAACCCCTGCGTCGATTTCTTCTTCCACATCGTACCCACCACCCCTGCCTCCTATTTCAACACCCAGCTCCCGCTTGCCTGGGCACACGACGACCTGACCACCCTCAAGCTCATCTGCAACCTCCGCGGCGTGCGGGGAACCGGAAAGTCCGACAAGGAGGGGTTCTACACGGCGGCGTTCTGGCTCCACAAACACCACCCCAAAACCCTCGCCTGCAACGTCGCATCCTTCGCCGAGTTCGGGTACTTTAAGGACTTGCCAGAGATCCTGTACCGCCTTCTAGAAGGCGAAGACGTGAGGAAGAAGCAAAAGGCAGAGTGGAATATCAGGAAAAGCGGGTCGGGTCGAACTAGGAGGGATAGGAGGATGCGAGGCCGCACTCGGCCTTTACGCTACAAACCGCCACCATCAACCTACGACAATTCACCATTCTATTCAGCATCGCATCAGCACCCGTGGGGTCTGAAACCCCCATTCAGCGGCGGCCGCGGCCGCAAGTCAAGTAATAAAAAATATGGTCGAGGGGATGAGCAATCTAAAGAGGCCAGGATACAACTGGCAAAGGAAAGGGCACAATCGGAGAAGGAAAAGGCAAGCCTTTTGAGGAATGAGAAGAAGGCTGCCATGGCCAAGAAAGCCATTGTGAGGTATCAGCGCGACCCTGATTTCCGATTTCTGTATGAGAGGGTTTCAGACCTTTTCGCAGAGTGTTTGAAGTCCGATATGGAAAATTTCAAGGCCAATCAGTACAAGAATATCAGCCTGGCAGCCAAATGGTGCCCTTCAATCGATTCCTCCTTCGATAAGGCCACTCTGTTGTGCGAAAGCATTGCCAATAAGGTTTTCCCAAGAGAATCGTACCTGGAGTACGAAGGGGTTGAAGAGGCACATTATGCGTACAGAGTGAGAGATCGGCTGAGGAAGGAGGTGTTGGTGCCGCTGAGGAAGGTGTTGGAGTTGCCAGAGGTTTATATCGGTGCTAATCAGTGGGGTTTGATTCCTTACAACCGAGTGGCCTCTGTGGCCATGAAGTTTTACAAGGAGAAATTCTTGAAGCACGATGAGGAGAGGTTTAAGAAGTATTTGGCGGATGTTAAGGCCGGAAAGTCCACCATTGCTGCAGGTGCTCTGCTTCCGCACGAGATCATAGAGTCTCTGAATCATGGAGATGGCGGTCAGGTGGCCGAGCTTCAATGGAAGAGAATGGTGGATGATCTGTTGAAGCAAGGGAAGATGAAGAACTGTTTGGCTGTGTGTGACGTCTCTGGCAGCATGAATGGGACCCCAATGGAGGTTTCCGTGGCTTTGGGATTGTTGGTGTCCGAGCTGATTGATGAGCCATGGAAGGGGAAGGTCATCACTTTCAGTCACAACCCTCAGCTGCATCAGATACAAGGCCATGATCTAAGCTCCAAGTGCAACTTTATAAGGACAATGGAATGGGGACGCAACACGAATTTCCAAAAGGTGTTTGATTTGATTCTCCGAGTGGCGGTGGACGGGAAGTTGAAGCCGGAGCAAATGATAAACAGGATCTTCGTGTTTAGCGACATGGAGTTTGATCAGGCTTCAAGTAGCAGGCATTGGGAGACTGATTACCAGGCTATACAGAGGAAGTTTACTGAGAAGGGGTACGGGAATGCGGTGCCGCAGATTATCTTTTGGAATCTGAGGCATTCGAAGTCTACGCCAATGCCGAGCAACCAACCAGGAGTGGCATTGTTGAGTGGCTTCTCCAAGAATTTGATGAAGTTGTTCTTGGACAACGATGGGGAAGTTCGCCCGGACCTTGTCATGGAAGCAGCCATCTCTGGAGAAGAATATCAGAAGCTTGTTGTGCTGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

682

Amino Acids

77.3

Weight (kDa)

9.25

Isoelectric Point (pI)

36.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2828 PF11443 55 - 478 2.3e-137 Domain of unknown function (DUF2828)
DUF7788 PF25043 480 - 659 1.5e-78 Domain of unknown function (DUF7788)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000255)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24780 AT5G13210 AT5G43390 AT5G43400
fragaria_vesca FvH4_4g23010 FvH4_5g12462 FvH4_5g12490 FvH4_5g12491 FvH4_5g20030 FvH4_5g20031 FvH4_5g21510 FvH4_6g30070
malus_domestica MD00G1095100.v1.1 MD05G1186800.v1.1 MD05G1259500.v1.1 MD05G1260200.v1.1 MD06G1159400.v1.1 MD06G1159500.v1.1 MD06G1162500.v1.1 MD06G1164600.v1.1 MD06G1187800.v1.1 MD06G1212600.v1.1 MD06G1212700.v1.1 MD13G1126800.v1.1 MD14G1168300.v1.1 MD14G1206600.v1.1 MD14G1223900.v1.1 MD14G1230800.v1.1 MD15G1240300.v1.1 MD15G1416800.v1.1 MD16G1127300.v1.1
prunus_persica Prupe.1G218700_v2.0.a1 Prupe.4G045100_v2.0.a1 Prupe.5G159300_v2.0.a1 Prupe.5G172200_v2.0.a1 Prupe.5G172300_v2.0.a1 Prupe.5G173200_v2.0.a1 Prupe.5G173300_v2.0.a1 Prupe.5G173400_v2.0.a1 Prupe.5G219000_v2.0.a1 Prupe.5G219100_v2.0.a1 Prupe.5G219200_v2.0.a1
pyrus_communis pycom05g24390 pycom06g19020 pycom10g15060 pycom10g24800 pycom13g11010 pycom16g10800 pycom16g10810 pycom17g22350 pycom17g22360
rosa_chinensis RchiOBHm_Chr2g0136111 RchiOBHm_Chr4g0429291 RchiOBHm_Chr7g0184981 RchiOBHm_Chr7g0184991 RchiOBHm_Chr7g0185001 RchiOBHm_Chr7g0204841
rosa_laevigata RLG00000003427 RLG00000004941 RLG00000004943 RLG00000004944 RLG00000004945 RLG00000004946 RLG00000007084 RLG00000013701 RLG00000019505 RLG00000019506
rosa_multiflora Rmu_co8294559.1_g000001 Rmu_co8375195.1_g000001 Rmu_co8450783.1_g000001 Rmu_co8519567.1_g000001 Rmu_sc0000054.1_g000008 Rmu_sc0001689.1_g000017 Rmu_sc0001801.1_g000027 Rmu_sc0002070.1_g000010 Rmu_sc0002180.1_g000022 Rmu_sc0002986.1_g000018 Rmu_sc0003221.1_g000027 Rmu_sc0005629.1_g000018 Rmu_sc0009898.1_g000002 Rmu_sc0018428.1_g000003 Rmu_sc0018428.1_g000005 Rmu_sc0022816.1_g000001 Rmu_sc0041781.1_g000001 Rmu_ssc0000289.1_g000007 Rmu_ssc0000289.1_g000008 Rmu_ssc0000289.1_g000010
rosa_roxburghii Rroxscaffold_2G00108460 Rroxscaffold_3G00252720 Rroxscaffold_3G00269290 Rroxscaffold_5G00349680 Rroxscaffold_5G00370900 Rroxscaffold_6G00415020
rosa_rugosa Rorug01G0043400 Rorug02G0328200 Rorug04G0234800 Rorug06G0465600 Rorug06G0465800 Rorug06G0465900 Rorug06G0466000 Rorug06G0466100 Rorug07G0086200 Rorug07G0086200
rosa_samantha Rh2BG386700 Rh2CG365900 Rh2DG402800 Rh4AG290400 Rh4BG296100 Rh4CG311900 Rh4DG293300 Rh7AG077000 Rh7AG077100 Rh7AG077200 Rh7AG216600 Rh7BG070200 Rh7BG070300 Rh7BG070400 Rh7BG213700 Rh7CG071400 Rh7CG071600 Rh7CG071700 Rh7CG071900 Rh7CG230100 Rh7DG070800 Rh7DG070900 Rh7DG224200 Rh7DG440100
rosa_wichuraiana Rw2G030970 Rw4G025150 Rw7G005840 Rw7G005850 Rw7G005860 Rw7G018770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1625
AarI CACCTGC 1 cut(s) 1310
AasI GACNNNNNNGTC 1 cut(s) 104
AatII GACGTC 1 cut(s) 1461
Acc36I ACCTGC 1 cut(s) 1310
AccB1I GGYRCC 4 cut(s) 173, 975, 1132, 1843
AccI GTMKAC 2 cut(s) 96, 1883
AccII CGCG 3 cut(s) 324, 687, 855
AccIII TCCGGA 1 cut(s) 28
AclWI GGATC 2 cut(s) 457, 1739
AcoI YGGCCR 5 cut(s) 682, 688, 825, 1221, 1380
AcsI RAATTY 4 cut(s) 928, 1244, 1654, 1939
AcyI GRCGYC 2 cut(s) 174, 1458
AfaI GTAC 8 cut(s) 231, 443, 470, 947, 1058, 1067, 1097, 1832
AfiI CCNNNNNNNGG 8 cut(s) 155, 438, 677, 843, 1489, 1544, 1690, 1993
AjiI CACGTC 1 cut(s) 493
AjnI CCWGG 6 cut(s) 275, 743, 960, 1059, 1797, 1906
AloI GAACNNNNNNTCC 2 cut(s) 161, 193
AluBI AGCT 8 cut(s) 34, 265, 307, 1387, 1525, 1579, 1608, 2029
AluI AGCT 8 cut(s) 34, 265, 307, 1387, 1525, 1579, 1608, 2029
Alw21I GWGCWC 2 cut(s) 36, 1327
Alw26I GTCTC 4 cut(s) 20, 1356, 1465, 1781
AlwI GGATC 2 cut(s) 457, 1739
Aor13HI TCCGGA 1 cut(s) 28
ApeKI GCWGC 8 cut(s) 71, 125, 818, 965, 1316, 1467, 1579, 2000
ApoI RAATTY 4 cut(s) 928, 1244, 1654, 1939
ArsI GACNNNNNNTTYG 2 cut(s) 1739, 1771
Asp700I GAANNNNTTC 1 cut(s) 1952
AspLEI GCGC 2 cut(s) 176, 855
AspS9I GGNCC 4 cut(s) 23, 39, 1479, 1984
AsuC2I CCSGG 1 cut(s) 1982
AsuHPI GGTGA 2 cut(s) 151, 615
AsuII TTCGAA 1 cut(s) 1877
AvaII GGWCC 4 cut(s) 23, 39, 1479, 1984
BaeGI GKGCMC 3 cut(s) 283, 772, 980
BalI TGGCCA 2 cut(s) 827, 1223
BanI GGYRCC 4 cut(s) 173, 975, 1132, 1843
BanII GRGCYC 1 cut(s) 36
BauI CACGAG 1 cut(s) 1337
BbsI GAAGAC 1 cut(s) 495
Bbv12I GWGCWC 2 cut(s) 36, 1327
BbvCI CCTCAGC 3 cut(s) 1115, 1139, 1575
BbvI GCAGC 8 cut(s) 83, 137, 805, 977, 1303, 1479, 1566, 2012
BccI CCATC 5 cut(s) 148, 613, 1361, 1961, 2012
BceAI ACGGC 2 cut(s) 187, 384
BcgI CGANNNNNNTGC 4 cut(s) 73, 107, 1010, 1044
BciT130I CCWGG 6 cut(s) 277, 745, 962, 1061, 1799, 1908
BciVI GTATCC 2 cut(s) 152, 741
BclI TGATCA 1 cut(s) 1759
BcnI CCSGG 1 cut(s) 1982
BcoDI GTCTC 4 cut(s) 20, 1356, 1465, 1781
BfaI CTAG 2 cut(s) 479, 549
BfmI CTRYAG 1 cut(s) 1317
BfoI RGCGCY 1 cut(s) 177
BfuAI ACCTGC 1 cut(s) 1310
BfuI GTATCC 2 cut(s) 152, 741
BglI GCCNNNNNGGC 1 cut(s) 1220
Bme1390I CCNGG 7 cut(s) 277, 745, 962, 1061, 1799, 1908, 1982
Bme18I GGWCC 4 cut(s) 23, 39, 1479, 1984
BmgBI CACGTC 1 cut(s) 493
BmgT120I GGNCC 4 cut(s) 23, 39, 1479, 1984
BmrFI CCNGG 7 cut(s) 277, 745, 962, 1061, 1799, 1908, 1982
BmsI GCATC 5 cut(s) 431, 555, 647, 652, 1591
BpiI GAAGAC 1 cut(s) 495
BpmI CTGGAG 2 cut(s) 1082, 2031
Bpu10I CCTNAGC 4 cut(s) 35, 1115, 1139, 1575
Bpu14I TTCGAA 1 cut(s) 1877
BpuEI CTTGAG 1 cut(s) 287
BpuMI CCSGG 1 cut(s) 1982
Bsa29I ATCGAT 1 cut(s) 987
BsaHI GRCGYC 2 cut(s) 174, 1458
BsaJI CCNNGG 9 cut(s) 19, 129, 276, 322, 653, 685, 822, 1497, 1538
BsaWI WCCGGW 3 cut(s) 28, 136, 338
BsaXI ACNNNNNCTCC 2 cut(s) 161, 191
Bsc4I CCNNNNNNNGG 8 cut(s) 155, 438, 677, 843, 1489, 1544, 1690, 1993
Bse1I ACTGG 1 cut(s) 759
Bse3DI GCAATG 2 cut(s) 1026, 1311
BseAI TCCGGA 1 cut(s) 28
BseBI CCWGG 6 cut(s) 277, 745, 962, 1061, 1799, 1908
BseCI ATCGAT 1 cut(s) 987
BseDI CCNNGG 9 cut(s) 19, 129, 276, 322, 653, 685, 822, 1497, 1538
BseGI GGATG 5 cut(s) 422, 570, 730, 1294, 1414
BseLI CCNNNNNNNGG 8 cut(s) 155, 438, 677, 843, 1489, 1544, 1690, 1993
BseMI GCAATG 2 cut(s) 1026, 1311
BseMII CTCAG 6 cut(s) 49, 1106, 1130, 1589, 1812, 1859
BseNI ACTGG 1 cut(s) 759
BseRI GAGGAG 2 cut(s) 8, 1277
BseSI GKGCMC 3 cut(s) 283, 772, 980
BseX3I CGGCCG 2 cut(s) 682, 688
BseXI GCAGC 8 cut(s) 83, 137, 805, 977, 1303, 1479, 1566, 2012
BseYI CCCAGC 1 cut(s) 261
Bsh1236I CGCG 3 cut(s) 324, 687, 855
Bsh1285I CGRYCG 2 cut(s) 685, 691
BshNI GGYRCC 4 cut(s) 173, 975, 1132, 1843
BshVI ATCGAT 1 cut(s) 987
BsiEI CGRYCG 2 cut(s) 685, 691
BsiHKAI GWGCWC 2 cut(s) 36, 1327
BsiSI CCGG 7 cut(s) 29, 137, 195, 339, 1299, 1712, 1982
BslFI GGGAC 2 cut(s) 1492, 1656
BslI CCNNNNNNNGG 8 cut(s) 155, 438, 677, 843, 1489, 1544, 1690, 1993
BsmAI GTCTC 4 cut(s) 20, 1356, 1465, 1781
BsmBI CGTCTC 1 cut(s) 1465
BsmFI GGGAC 2 cut(s) 1492, 1656
BsmI GAATGC 2 cut(s) 1843, 1873
Bsp119I TTCGAA 1 cut(s) 1877
Bsp1286I GDGCHC 5 cut(s) 36, 283, 772, 980, 1327
Bsp13I TCCGGA 1 cut(s) 28
Bsp143I GATC 8 cut(s) 141, 462, 1108, 1341, 1411, 1600, 1731, 1759
Bsp19I CCATGG 2 cut(s) 822, 1538
BspCNI CTCAG 6 cut(s) 48, 1107, 1131, 1588, 1813, 1860
BspDI ATCGAT 1 cut(s) 987
BspEI TCCGGA 1 cut(s) 28
BspFNI CGCG 3 cut(s) 324, 687, 855
BspMAI CTGCAG 1 cut(s) 1321
BspMI ACCTGC 1 cut(s) 1310
BspPI GGATC 2 cut(s) 457, 1739
BspT104I TTCGAA 1 cut(s) 1877
BspT107I GGYRCC 4 cut(s) 173, 975, 1132, 1843
BsrDI GCAATG 2 cut(s) 1026, 1311
BsrI ACTGG 1 cut(s) 759
BssECI CCNNGG 9 cut(s) 19, 129, 276, 322, 653, 685, 822, 1497, 1538
BssMI GATC 8 cut(s) 141, 462, 1108, 1341, 1411, 1600, 1731, 1759
BssNI GRCGYC 2 cut(s) 174, 1458
BssSI CACGAG 1 cut(s) 1337
BssT1I CCWWGG 3 cut(s) 129, 822, 1538
Bst2BI CACGAG 1 cut(s) 1337
Bst2UI CCWGG 6 cut(s) 277, 745, 962, 1061, 1799, 1908
Bst4CI ACNGT 1 cut(s) 1442
Bst6I CTCTTC 2 cut(s) 1074, 1391
BstACI GRCGYC 2 cut(s) 174, 1458
BstAPI GCANNNNNTGC 2 cut(s) 204, 1091
BstBI TTCGAA 1 cut(s) 1877
BstC8I GCNNGC 5 cut(s) 273, 329, 412, 793, 1778
BstDEI CTNAG 7 cut(s) 35, 1115, 1139, 1575, 1604, 1821, 1868
BstDSI CCRYGG 6 cut(s) 322, 653, 685, 822, 1497, 1538
BstF5I GGATG 5 cut(s) 422, 570, 730, 1294, 1414
BstFNI CGCG 3 cut(s) 324, 687, 855
BstH2I RGCGCY 1 cut(s) 177
BstHHI GCGC 2 cut(s) 176, 855
BstKTI GATC 8 cut(s) 144, 465, 1111, 1344, 1414, 1603, 1734, 1762
BstMAI GTCTC 4 cut(s) 20, 1356, 1465, 1781
BstMBI GATC 8 cut(s) 141, 462, 1108, 1341, 1411, 1600, 1731, 1759
BstMCI CGRYCG 2 cut(s) 685, 691
BstNI CCWGG 6 cut(s) 277, 745, 962, 1061, 1799, 1908
BstSCI CCNGG 7 cut(s) 275, 743, 960, 1059, 1797, 1906, 1980
BstSFI CTRYAG 1 cut(s) 1317
BstSLI GKGCMC 3 cut(s) 283, 772, 980
BstUI CGCG 3 cut(s) 324, 687, 855
BstV1I GCAGC 8 cut(s) 83, 137, 805, 977, 1303, 1479, 1566, 2012
BstV2I GAAGAC 1 cut(s) 495
BstX2I RGATCY 2 cut(s) 462, 1731
BstYI RGATCY 2 cut(s) 462, 1731
BstZI CGGCCG 2 cut(s) 682, 688
Bsu15I ATCGAT 1 cut(s) 987
BsuI GTATCC 2 cut(s) 152, 741
BsuTUI ATCGAT 1 cut(s) 987
BtgI CCRYGG 6 cut(s) 322, 653, 685, 822, 1497, 1538
BtgZI GCGATG 1 cut(s) 624
BtrI CACGTC 1 cut(s) 493
BtsCI GGATG 5 cut(s) 422, 570, 730, 1294, 1414
BtsIMutI CAGTG 1 cut(s) 1190
BveI ACCTGC 1 cut(s) 1310
Cac8I GCNNGC 5 cut(s) 273, 329, 412, 793, 1778
CciNI GCGGCCGC 2 cut(s) 682, 688
CfoI GCGC 2 cut(s) 176, 855
Cfr13I GGNCC 4 cut(s) 23, 39, 1479, 1984
Cfr42I CCGCGG 2 cut(s) 325, 688
ClaI ATCGAT 1 cut(s) 987
CseI GACGC 3 cut(s) 131, 196, 1653
Csp6I GTAC 8 cut(s) 230, 442, 469, 946, 1057, 1066, 1096, 1831
CviAII CATG 9 cut(s) 103, 823, 1225, 1361, 1471, 1539, 1598, 1750, 1993
CviQI GTAC 8 cut(s) 230, 442, 469, 946, 1057, 1066, 1096, 1831
DdeI CTNAG 7 cut(s) 35, 1115, 1139, 1575, 1604, 1821, 1868
DinI GGCGCC 1 cut(s) 175
DpnI GATC 8 cut(s) 143, 464, 1110, 1343, 1413, 1602, 1733, 1761
DpnII GATC 8 cut(s) 141, 462, 1108, 1341, 1411, 1600, 1731, 1759
DrdI GACNNNNNNGTC 1 cut(s) 104
DseDI GACNNNNNNGTC 1 cut(s) 104
EaeI YGGCCR 5 cut(s) 682, 688, 825, 1221, 1380
EagI CGGCCG 2 cut(s) 682, 688
Eam1104I CTCTTC 2 cut(s) 1074, 1391
EarI CTCTTC 2 cut(s) 1074, 1391
EciI GGCGGA 1 cut(s) 1301
Ecl136II GAGCTC 1 cut(s) 34
EclXI CGGCCG 2 cut(s) 682, 688
Eco130I CCWWGG 3 cut(s) 129, 822, 1538
Eco24I GRGCYC 1 cut(s) 36
Eco47I GGWCC 4 cut(s) 23, 39, 1479, 1984
Eco52I CGGCCG 2 cut(s) 682, 688
Eco53kI GAGCTC 1 cut(s) 34
EcoICRI GAGCTC 1 cut(s) 34
EcoO109I RGGNCCY 2 cut(s) 39, 1479
EcoRII CCWGG 6 cut(s) 275, 743, 960, 1059, 1797, 1906
EcoT14I CCWWGG 3 cut(s) 129, 822, 1538
EcoT38I GRGCYC 1 cut(s) 36
EgeI GGCGCC 1 cut(s) 175
EheI GGCGCC 1 cut(s) 175
ErhI CCWWGG 3 cut(s) 129, 822, 1538
Esp3I CGTCTC 1 cut(s) 1465
FaeI CATG 9 cut(s) 106, 826, 1228, 1364, 1474, 1542, 1601, 1753, 1996
FalI AAGNNNNNCTT 4 cut(s) 1604, 1636, 1940, 1972
FaqI GGGAC 2 cut(s) 1492, 1656
FatI CATG 9 cut(s) 102, 822, 1224, 1360, 1470, 1538, 1597, 1749, 1992
FauI CCCGC 4 cut(s) 76, 276, 324, 527
FbaI TGATCA 1 cut(s) 1759
FblI GTMKAC 2 cut(s) 96, 1883
FokI GGATG 5 cut(s) 409, 577, 737, 1301, 1421
FriOI GRGCYC 1 cut(s) 36
FspBI CTAG 2 cut(s) 479, 549
GlaI GCGC 2 cut(s) 175, 854
GsaI CCCAGC 1 cut(s) 265
GsuI CTGGAG 2 cut(s) 1082, 2031
HaeII RGCGCY 1 cut(s) 177
HapII CCGG 7 cut(s) 29, 137, 195, 339, 1299, 1712, 1982
HgaI GACGC 3 cut(s) 131, 196, 1653
HhaI GCGC 2 cut(s) 176, 855
Hin1I GRCGYC 2 cut(s) 174, 1458
Hin1II CATG 9 cut(s) 106, 826, 1228, 1364, 1474, 1542, 1601, 1753, 1996
Hin6I GCGC 2 cut(s) 174, 853
HinP1I GCGC 2 cut(s) 174, 853
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HindIII AAGCTT 1 cut(s) 2027
HinfI GANTC 7 cut(s) 989, 1052, 1195, 1349, 1357, 1678, 1864
HpaII CCGG 7 cut(s) 29, 137, 195, 339, 1299, 1712, 1982
HphI GGTGA 2 cut(s) 151, 615
Hpy166II GTNNAC 5 cut(s) 97, 1308, 1696, 1818, 1884
Hpy188III TCNNGA 6 cut(s) 29, 37, 479, 526, 1249, 2010
Hpy8I GTNNAC 5 cut(s) 97, 1308, 1696, 1818, 1884
Hpy99I CGWCG 5 cut(s) 98, 125, 212, 290, 422
HpyCH4III ACNGT 1 cut(s) 1442
HpyCH4IV ACGT 4 cut(s) 182, 417, 492, 1458
HpyCH4V TGCA 5 cut(s) 315, 414, 1319, 1582, 1617
HpyF3I CTNAG 7 cut(s) 35, 1115, 1139, 1575, 1604, 1821, 1868
HpySE526I ACGT 4 cut(s) 182, 417, 492, 1458
Hsp92I GRCGYC 2 cut(s) 174, 1458
Hsp92II CATG 9 cut(s) 106, 826, 1228, 1364, 1474, 1542, 1601, 1753, 1996
HspAI GCGC 2 cut(s) 174, 853
KasI GGCGCC 1 cut(s) 173
KflI GGGWCCC 1 cut(s) 1479
Kpn2I TCCGGA 1 cut(s) 28
Ksp22I TGATCA 1 cut(s) 1759
KspI CCGCGG 2 cut(s) 325, 688
Kzo9I GATC 8 cut(s) 141, 462, 1108, 1341, 1411, 1600, 1731, 1759
LmnI GCTCC 6 cut(s) 10, 31, 270, 387, 1613, 1714
Lsp1109I GCAGC 8 cut(s) 83, 137, 805, 977, 1303, 1479, 1566, 2012
LweI GCATC 5 cut(s) 431, 555, 647, 652, 1591
MaeI CTAG 2 cut(s) 479, 549
MaeII ACGT 4 cut(s) 182, 417, 492, 1458
MaeIII GTNAC 2 cut(s) 1454, 1565
MalI GATC 8 cut(s) 143, 464, 1110, 1343, 1413, 1602, 1733, 1761
MboI GATC 8 cut(s) 141, 462, 1108, 1341, 1411, 1600, 1731, 1759
MflI RGATCY 2 cut(s) 462, 1731
MhlI GDGCHC 5 cut(s) 36, 283, 772, 980, 1327
MlsI TGGCCA 2 cut(s) 827, 1223
MluCI AATT 6 cut(s) 112, 619, 928, 1244, 1654, 1939
MluNI TGGCCA 2 cut(s) 827, 1223
Mly113I GGCGCC 1 cut(s) 174
MlyI GAGTC 1 cut(s) 1358
MmeI TCCRAC 2 cut(s) 372, 1131
Mox20I TGGCCA 2 cut(s) 827, 1223
MroI TCCGGA 1 cut(s) 28
MroXI GAANNNNTTC 1 cut(s) 1952
MscI TGGCCA 2 cut(s) 827, 1223
MseI TTAA 3 cut(s) 447, 1272, 1293
MslI CAYNNNNRTG 1 cut(s) 1365
Msp20I TGGCCA 2 cut(s) 827, 1223
MspA1I CMGCKG 5 cut(s) 324, 678, 687, 1138, 1579
MspI CCGG 7 cut(s) 29, 137, 195, 339, 1299, 1712, 1982
MspR9I CCNGG 7 cut(s) 277, 745, 962, 1061, 1799, 1908, 1982
Mva1269I GAATGC 2 cut(s) 1843, 1873
MvaI CCWGG 6 cut(s) 277, 745, 962, 1061, 1799, 1908
MvnI CGCG 3 cut(s) 324, 687, 855
NarI GGCGCC 1 cut(s) 174
NciI CCSGG 1 cut(s) 1982
NcoI CCATGG 2 cut(s) 822, 1538
NdeII GATC 8 cut(s) 141, 462, 1108, 1341, 1411, 1600, 1731, 1759
NlaIII CATG 9 cut(s) 106, 826, 1228, 1364, 1474, 1542, 1601, 1753, 1996
NmeAIII GCCGAG 4 cut(s) 457, 559, 1408, 1920
NmuCI GTSAC 2 cut(s) 1454, 1565
NotI GCGGCCGC 2 cut(s) 682, 688
NspV TTCGAA 1 cut(s) 1877
PaqCI CACCTGC 1 cut(s) 1310
PcsI WCGNNNNNNNCGW 3 cut(s) 414, 1743, 1892
PctI GAATGC 2 cut(s) 1843, 1873
PdmI GAANNNNTTC 1 cut(s) 1952
PfeI GAWTC 6 cut(s) 989, 1052, 1195, 1357, 1678, 1864
PflFI GACNNNGTC 1 cut(s) 1988
PleI GAGTC 1 cut(s) 1357
PluTI GGCGCC 1 cut(s) 177
PpsI GAGTC 1 cut(s) 1357
PpuMI RGGWCCY 2 cut(s) 39, 1479
PsiI TTATAA 1 cut(s) 1625
Psp124BI GAGCTC 1 cut(s) 36
Psp5II RGGWCCY 2 cut(s) 39, 1479
Psp6I CCWGG 6 cut(s) 275, 743, 960, 1059, 1797, 1906
PspFI CCCAGC 1 cut(s) 261
PspGI CCWGG 6 cut(s) 275, 743, 960, 1059, 1797, 1906
PspPI GGNCC 4 cut(s) 23, 39, 1479, 1984
PspPPI RGGWCCY 2 cut(s) 39, 1479
PstI CTGCAG 1 cut(s) 1321
PsuI RGATCY 2 cut(s) 462, 1731
PsyI GACNNNGTC 1 cut(s) 1988
PvuII CAGCTG 1 cut(s) 1579
RsaI GTAC 8 cut(s) 231, 443, 470, 947, 1058, 1067, 1097, 1832
RsaNI GTAC 8 cut(s) 230, 442, 469, 946, 1057, 1066, 1096, 1831
RseI CAYNNNNRTG 1 cut(s) 1365
SacI GAGCTC 1 cut(s) 36
SacII CCGCGG 2 cut(s) 325, 688
SalI GTCGAC 1 cut(s) 95
SaqAI TTAA 3 cut(s) 447, 1272, 1293
Sau3AI GATC 8 cut(s) 141, 462, 1108, 1341, 1411, 1600, 1731, 1759
Sau96I GGNCC 4 cut(s) 23, 39, 1479, 1984
SchI GAGTC 1 cut(s) 1358
ScrFI CCNGG 7 cut(s) 277, 745, 962, 1061, 1799, 1908, 1982
SduI GDGCHC 5 cut(s) 36, 283, 772, 980, 1327
SfaNI GCATC 5 cut(s) 431, 555, 647, 652, 1591
SfcI CTRYAG 1 cut(s) 1317
SfiI GGCCNNNNNGGCC 1 cut(s) 1220
SfoI GGCGCC 1 cut(s) 175
Sfr303I CCGCGG 2 cut(s) 325, 688
SfuI TTCGAA 1 cut(s) 1877
SgrBI CCGCGG 2 cut(s) 325, 688
SinI GGWCC 4 cut(s) 23, 39, 1479, 1984
SmiMI CAYNNNNRTG 1 cut(s) 1365
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
Sse9I AATT 6 cut(s) 112, 619, 928, 1244, 1654, 1939
SspDI GGCGCC 1 cut(s) 173
SspMI CTAG 2 cut(s) 479, 549
SstI GAGCTC 1 cut(s) 36
StyD4I CCNGG 7 cut(s) 275, 743, 960, 1059, 1797, 1906, 1980
StyI CCWWGG 3 cut(s) 129, 822, 1538
TaaI ACNGT 1 cut(s) 1442
TaiI ACGT 4 cut(s) 185, 420, 495, 1461
TaqI TCGA 7 cut(s) 96, 210, 544, 718, 987, 999, 1877
TaqII GACCGA 1 cut(s) 11
TasI AATT 6 cut(s) 112, 619, 928, 1244, 1654, 1939
TatI WGTACW 1 cut(s) 945
TfiI GAWTC 6 cut(s) 989, 1052, 1195, 1357, 1678, 1864
Tru1I TTAA 3 cut(s) 447, 1272, 1293
Tru9I TTAA 3 cut(s) 447, 1272, 1293
TscAI CASTG 1 cut(s) 1190
TseFI GTSAC 2 cut(s) 1454, 1565
TseI GCWGC 8 cut(s) 71, 125, 818, 965, 1316, 1467, 1579, 2000
Tsp45I GTSAC 2 cut(s) 1454, 1565
TspDTI ATGAA 4 cut(s) 1241, 1448, 1487, 1961
TspGWI ACGGA 1 cut(s) 1486
TspRI CASTG 1 cut(s) 1190
Tth111I GACNNNGTC 1 cut(s) 1988
VpaK11BI GGWCC 4 cut(s) 23, 39, 1479, 1984
XapI RAATTY 4 cut(s) 928, 1244, 1654, 1939
XbaI TCTAGA 1 cut(s) 478
XcmI CCANNNNNNNNNTGG 1 cut(s) 751
XmiI GTMKAC 2 cut(s) 96, 1883
XmnI GAANNNNTTC 1 cut(s) 1952
XspI CTAG 2 cut(s) 479, 549
ZraI GACGTC 1 cut(s) 1459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.