Rorug06G0466000

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000255
Physical Location & Seq
Forward (+)
59045333 .. 59051238
5906 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0466000.1

Sequence Viewer

Length: 2187 bp
ATGAAAAATAGTCAAATGGCTCTCTCGGCCATCGCTACTCTCACTCGCTTCTCAACTACTACCACTTCTTCTTCTTCTTCTCACTTCCACTGCATCAGTAAACGGCTCAGACTCATCGCCAGAGCACCAACCAAAAGTCAGCACCGCCAGAAAATGACGTCATCTTCTCCGGAAGCCAAGCAAGAGAAAATCGCAGCTCCTTTCGGCTCGTGGAAGTCCCCAATCACCGCCGATGTCGTCTCCGGCGCCTCCAAGCGGCTCGGCGGCACCGCCGTAGATTCCCACGGCCGCCTCATCTGGCTCGAGTCTCGTCCGTCGGAATCCGGGCGAGCGGTTCTGGTGAGAGAACCGGAGAGACCGGAAGACGAACCGGTTGATATCACGCCGAAGGACTACGCGGTTCGAACTCTGGCTCAGGAGTACGGCGGAGGCGCCTTCAGCGTTTCAGGAGATACACTTGTCTTCTCAAATTATAAGGATCAACGCCTTTACAAGCAGTCTTTAAGCTCCAAAGATTCTTCACCAGTACCACTCACTCCAGATTATGGTGGACCAGTTGTTAGTTATGCTGATGGGGTGTTTGATGCACGTTTTAACCGTTTTATCACTGTGAGGGAAGATTGTCGCGAAAGCAGCATGAATTCAATCACAACAATTGTAACAGTAAGGCTTGATGGAAAGGACATTCAGGAATCAGAAACACTCGTAGGTGGATGTGACTTCTATGCCTTCCCGCGTCTGGATGCCAAAGGAGAACGACTTGCATGGGTTGAATGGTGCCATCCTAACATGCCATGGGATATGTCTCAACTTTGGGTTGGGTATATATCTGAGAAAGGGGAAGTCTCAAAACGAGTCTGTGTTTCTGGCTCTGATCCTGCAATTAAGGAATCTCCAATTGAACCCAAGTGGTCCGCAAAAGGGGAACTATTTTTCATTACTGACAGAAAAAGTGGGTTTTGGAATCTCTATAAATGGGTTGAGTCTACTAATGAGGTCATATCAGTTTATTCTTTGGATGCTGAATTTTCCAAACCATTATGGGTTTTTGGCTTGAATTCTTATGAATTTATTGAGAGCCATGAACAAAAGAACCTTATTGCTTGCAGCTATAGGAAAAACGGGAGATCATATCTTGGAATTGTGGATGATGTTCAGGGCTCATTATCCCTGCTTAATATTCCTTTCACGGATATTGATAATATTACTTTGGGGATTAATTGCCTATATGTGGAAGGAGCATCAGAAGTTCATCCGTTGTCTGTTGCTAAGGTGACATTAGATGATCAAAAATCAAAAGCAGTTGAATTCAAGATTACTTGGTCTTCTTCACCCGATTGCTTAAAATATGAGTCCTACTTCAGCCTACCTGAATTAATTGAGTTCCCAACAGAAGTTCCTGGTCAAACTGCCTTTGCATACTTTTATCCACCATCAAATCCTAGCTACCAAGCAACTAAGGACGAAAAGCCTCCACTATTACTGAAAAGCCATGGAGGACCCACAGCTGAAGCACGCGGAATTTTAAATCTAAGTGTTCAGTACTGGACTAGTCGTGGTTGGGCATTCGTTGATGTTAACTATGGTGGAAGCACTGGTTATGGCAGAGAATATCGTGAAAGGCTGTTGAAGAAATGGGGAATTGTGGATGTAAATGATTGTTGCAGCTGTGCTAGTTATCTGGTGGAATCTGGAAAGGTAGATGGGAAACGACTTTGTATTACTGGAGGCTCTGCAGGTGGATACACAACCTTAGCTGCACTTGCATTTCGAGACACCTTCAAGGCTGGAGCTTCCTTGTATGGTGTAGCTGACTTAAACATGTTGAGGGCCGAAACTCACAAATTTGAATCTCATTATATGGATAATCTTGTTGGTAGTGATCAGGATTTCTTTGATAGATCACCAATCAACTTTGTTGATAAATTTTCTTGCCCAATAATCTTATTCCAAGGATTGGAAGACAAGGTTGTACCCCCAGATCAAGCTCGTACAATTTACCGTGCTCTGAAGGAAAAGGGCTTGCCAGTTGCTCTCGTGGAGTATGAAGGAGAACAACATGGTTTCCGCAAGGCTGAGAACATTATATTCACTCTGGAACAGCAAATGGTTTTCTTTGCACGTTTAGTTGGACACTTCAAGGTTGCAGATGAGATTACTCCGATCAAAATTGATAACTTGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

728

Amino Acids

81.18

Weight (kDa)

5.8

Isoelectric Point (pI)

39.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S9 PF00326 512 - 711 2.7e-47 Prolyl oligopeptidase family
BD-FAE PF20434 550 - 670 8.5e-09 BD-FAE
DLH PF01738 559 - 695 5.4e-09 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000255)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24780 AT5G13210 AT5G43390 AT5G43400
fragaria_vesca FvH4_4g23010 FvH4_5g12462 FvH4_5g12490 FvH4_5g12491 FvH4_5g20030 FvH4_5g20031 FvH4_5g21510 FvH4_6g30070
malus_domestica MD00G1095100.v1.1 MD05G1186800.v1.1 MD05G1259500.v1.1 MD05G1260200.v1.1 MD06G1159400.v1.1 MD06G1159500.v1.1 MD06G1162500.v1.1 MD06G1164600.v1.1 MD06G1187800.v1.1 MD06G1212600.v1.1 MD06G1212700.v1.1 MD13G1126800.v1.1 MD14G1168300.v1.1 MD14G1206600.v1.1 MD14G1223900.v1.1 MD14G1230800.v1.1 MD15G1240300.v1.1 MD15G1416800.v1.1 MD16G1127300.v1.1
prunus_persica Prupe.1G218700_v2.0.a1 Prupe.4G045100_v2.0.a1 Prupe.5G159300_v2.0.a1 Prupe.5G172200_v2.0.a1 Prupe.5G172300_v2.0.a1 Prupe.5G173200_v2.0.a1 Prupe.5G173300_v2.0.a1 Prupe.5G173400_v2.0.a1 Prupe.5G219000_v2.0.a1 Prupe.5G219100_v2.0.a1 Prupe.5G219200_v2.0.a1
pyrus_communis pycom05g24390 pycom06g19020 pycom10g15060 pycom10g24800 pycom13g11010 pycom16g10800 pycom16g10810 pycom17g22350 pycom17g22360
rosa_chinensis RchiOBHm_Chr2g0136111 RchiOBHm_Chr4g0429291 RchiOBHm_Chr7g0184981 RchiOBHm_Chr7g0184991 RchiOBHm_Chr7g0185001 RchiOBHm_Chr7g0204841
rosa_laevigata RLG00000003427 RLG00000004941 RLG00000004943 RLG00000004944 RLG00000004945 RLG00000004946 RLG00000007084 RLG00000013701 RLG00000019505 RLG00000019506
rosa_multiflora Rmu_co8294559.1_g000001 Rmu_co8375195.1_g000001 Rmu_co8450783.1_g000001 Rmu_co8519567.1_g000001 Rmu_sc0000054.1_g000008 Rmu_sc0001689.1_g000017 Rmu_sc0001801.1_g000027 Rmu_sc0002070.1_g000010 Rmu_sc0002180.1_g000022 Rmu_sc0002986.1_g000018 Rmu_sc0003221.1_g000027 Rmu_sc0005629.1_g000018 Rmu_sc0009898.1_g000002 Rmu_sc0018428.1_g000003 Rmu_sc0018428.1_g000005 Rmu_sc0022816.1_g000001 Rmu_sc0041781.1_g000001 Rmu_ssc0000289.1_g000007 Rmu_ssc0000289.1_g000008 Rmu_ssc0000289.1_g000010
rosa_roxburghii Rroxscaffold_2G00108460 Rroxscaffold_3G00252720 Rroxscaffold_3G00269290 Rroxscaffold_5G00349680 Rroxscaffold_5G00370900 Rroxscaffold_6G00415020
rosa_rugosa Rorug01G0043400 Rorug02G0328200 Rorug04G0234800 Rorug06G0465600 Rorug06G0465800 Rorug06G0465900 Rorug06G0466000 Rorug06G0466100 Rorug07G0086200 Rorug07G0086200
rosa_samantha Rh2BG386700 Rh2CG365900 Rh2DG402800 Rh4AG290400 Rh4BG296100 Rh4CG311900 Rh4DG293300 Rh7AG077000 Rh7AG077100 Rh7AG077200 Rh7AG216600 Rh7BG070200 Rh7BG070300 Rh7BG070400 Rh7BG213700 Rh7CG071400 Rh7CG071600 Rh7CG071700 Rh7CG071900 Rh7CG230100 Rh7DG070800 Rh7DG070900 Rh7DG224200 Rh7DG440100
rosa_wichuraiana Rw2G030970 Rw4G025150 Rw7G005840 Rw7G005850 Rw7G005860 Rw7G018770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 474
AarI CACCTGC 1 cut(s) 1727
AatII GACGTC 1 cut(s) 161
Acc36I ACCTGC 1 cut(s) 1727
AccB1I GGYRCC 4 cut(s) 245, 266, 431, 777
AccB7I CCANNNNNTGG 2 cut(s) 545, 1957
AccBSI CCGCTC 1 cut(s) 332
AccI GTMKAC 1 cut(s) 986
AccII CGCG 4 cut(s) 398, 627, 736, 1518
AccIII TCCGGA 1 cut(s) 169
AclWI GGATC 2 cut(s) 486, 869
AcoI YGGCCR 2 cut(s) 27, 286
AcsI RAATTY 8 cut(s) 640, 1025, 1057, 1067, 1307, 1521, 1844, 1925
AcuI CTGAAG 4 cut(s) 421, 1345, 1530, 2030
AcyI GRCGYC 3 cut(s) 158, 246, 432
AfaI GTAC 5 cut(s) 422, 528, 1544, 1974, 1993
AfiI CCNNNNNNNGG 6 cut(s) 255, 545, 739, 921, 1231, 1957
AflIII ACRYGT 1 cut(s) 1821
AgeI ACCGGT 1 cut(s) 370
AhlI ACTAGT 1 cut(s) 1550
AjnI CCWGG 1 cut(s) 1399
Alw21I GWGCWC 2 cut(s) 127, 2008
Alw26I GTCTC 6 cut(s) 244, 312, 349, 810, 850, 1767
AlwI GGATC 2 cut(s) 486, 869
Ama87I CYCGRG 1 cut(s) 302
Aor13HI TCCGGA 1 cut(s) 169
AoxI GGCC 3 cut(s) 27, 286, 1830
ApeKI GCWGC 5 cut(s) 194, 633, 1107, 1665, 1757
ApoI RAATTY 8 cut(s) 640, 1025, 1057, 1067, 1307, 1521, 1844, 1925
AseI ATTAAT 2 cut(s) 1218, 1376
AsiGI ACCGGT 1 cut(s) 370
AspLEI GCGC 2 cut(s) 248, 434
AspS9I GGNCC 4 cut(s) 551, 912, 1499, 1830
AsuC2I CCSGG 1 cut(s) 325
AsuHPI GGTGA 6 cut(s) 217, 352, 513, 1285, 1323, 1896
AsuII TTCGAA 1 cut(s) 403
AvaI CYCGRG 1 cut(s) 302
AvaII GGWCC 3 cut(s) 551, 912, 1499
BaeI ACNNNNGTAYC 2 cut(s) 444, 477
BanI GGYRCC 4 cut(s) 245, 266, 431, 777
BanII GRGCYC 1 cut(s) 1163
BauI CACGAG 2 cut(s) 208, 2036
BbsI GAAGAC 4 cut(s) 369, 454, 1317, 1968
Bbv12I GWGCWC 2 cut(s) 127, 2008
BbvI GCAGC 5 cut(s) 206, 645, 1119, 1677, 1744
BccI CCATC 6 cut(s) 38, 566, 668, 789, 1441, 1697
BceAI ACGGC 4 cut(s) 119, 257, 301, 439
BciT130I CCWGG 1 cut(s) 1401
BciVI GTATCC 1 cut(s) 1736
BclI TGATCA 2 cut(s) 1285, 1882
BcnI CCSGG 1 cut(s) 325
BcoDI GTCTC 6 cut(s) 244, 312, 349, 810, 850, 1767
BcuI ACTAGT 1 cut(s) 1550
BfaI CTAG 3 cut(s) 1443, 1551, 1674
BfmI CTRYAG 2 cut(s) 1111, 1734
BfoI RGCGCY 2 cut(s) 249, 435
BfuAI ACCTGC 1 cut(s) 1727
BfuI GTATCC 1 cut(s) 1736
BisI GCNGC 8 cut(s) 195, 257, 265, 289, 634, 1108, 1666, 1758
BlsI GCNGC 8 cut(s) 196, 258, 266, 290, 635, 1109, 1667, 1759
BmcAI AGTACT 1 cut(s) 1544
Bme1390I CCNGG 2 cut(s) 325, 1401
Bme18I GGWCC 3 cut(s) 551, 912, 1499
BmeT110I CYCGRG 1 cut(s) 302
BmgT120I GGNCC 4 cut(s) 551, 912, 1499, 1830
BmiI GGNNCC 5 cut(s) 247, 268, 433, 779, 1501
BmrFI CCNGG 2 cut(s) 325, 1401
BmsI GCATC 5 cut(s) 102, 574, 733, 1009, 1250
BpiI GAAGAC 4 cut(s) 369, 454, 1317, 1968
BpmI CTGGAG 3 cut(s) 522, 1746, 1809
Bpu10I CCTNAGC 3 cut(s) 414, 1269, 1753
Bpu14I TTCGAA 1 cut(s) 403
BpuMI CCSGG 1 cut(s) 325
BsaHI GRCGYC 3 cut(s) 158, 246, 432
BsaI GGTCTC 1 cut(s) 349
BsaJI CCNNGG 4 cut(s) 283, 794, 1492, 1951
BsaWI WCCGGW 4 cut(s) 169, 349, 358, 370
Bsc4I CCNNNNNNNGG 6 cut(s) 255, 545, 739, 921, 1231, 1957
Bse118I RCCGGY 1 cut(s) 370
Bse1I ACTGG 6 cut(s) 524, 554, 1550, 1600, 1729, 2027
BseAI TCCGGA 1 cut(s) 169
BseBI CCWGG 1 cut(s) 1401
BseDI CCNNGG 4 cut(s) 283, 794, 1492, 1951
BseGI GGATG 7 cut(s) 719, 748, 781, 1024, 1153, 1252, 1654
BseLI CCNNNNNNNGG 6 cut(s) 255, 545, 739, 921, 1231, 1957
BseMII CTCAG 4 cut(s) 121, 428, 822, 2067
BseNI ACTGG 6 cut(s) 524, 554, 1550, 1600, 1729, 2027
BseX3I CGGCCG 1 cut(s) 286
BseXI GCAGC 5 cut(s) 206, 645, 1119, 1677, 1744
BsgI GTGCAG 1 cut(s) 1743
Bsh1236I CGCG 4 cut(s) 398, 627, 736, 1518
Bsh1285I CGRYCG 1 cut(s) 289
BshFI GGCC 3 cut(s) 29, 288, 1832
BshNI GGYRCC 4 cut(s) 245, 266, 431, 777
BshTI ACCGGT 1 cut(s) 370
BsiEI CGRYCG 1 cut(s) 289
BsiHKAI GWGCWC 2 cut(s) 127, 2008
BsiHKCI CYCGRG 1 cut(s) 302
BsiSI CCGG 6 cut(s) 170, 243, 324, 350, 359, 371
BslFI GGGAC 1 cut(s) 202
BslI CCNNNNNNNGG 6 cut(s) 255, 545, 739, 921, 1231, 1957
BsmAI GTCTC 6 cut(s) 244, 312, 349, 810, 850, 1767
BsmBI CGTCTC 1 cut(s) 244
BsmFI GGGAC 1 cut(s) 202
BsmI GAATGC 1 cut(s) 1565
BsnI GGCC 3 cut(s) 29, 288, 1832
Bso31I GGTCTC 1 cut(s) 349
BsoBI CYCGRG 1 cut(s) 302
Bsp119I TTCGAA 1 cut(s) 403
Bsp1286I GDGCHC 3 cut(s) 127, 1163, 2008
Bsp13I TCCGGA 1 cut(s) 169
Bsp143I GATC 8 cut(s) 478, 874, 1127, 1285, 1882, 1901, 1981, 2163
Bsp19I CCATGG 2 cut(s) 794, 1492
Bsp68I TCGCGA 1 cut(s) 627
BspANI GGCC 3 cut(s) 29, 288, 1832
BspCNI CTCAG 4 cut(s) 120, 427, 823, 2068
BspEI TCCGGA 1 cut(s) 169
BspFNI CGCG 4 cut(s) 398, 627, 736, 1518
BspLI GGNNCC 5 cut(s) 247, 268, 433, 779, 1501
BspMAI CTGCAG 1 cut(s) 1738
BspMI ACCTGC 1 cut(s) 1727
BspPI GGATC 2 cut(s) 486, 869
BspT104I TTCGAA 1 cut(s) 403
BspT107I GGYRCC 4 cut(s) 245, 266, 431, 777
BspTNI GGTCTC 1 cut(s) 349
BsrBI CCGCTC 1 cut(s) 332
BsrFI RCCGGY 1 cut(s) 370
BsrI ACTGG 6 cut(s) 524, 554, 1550, 1600, 1729, 2027
BssAI RCCGGY 1 cut(s) 370
BssECI CCNNGG 4 cut(s) 283, 794, 1492, 1951
BssMI GATC 8 cut(s) 478, 874, 1127, 1285, 1882, 1901, 1981, 2163
BssNI GRCGYC 3 cut(s) 158, 246, 432
BssSI CACGAG 2 cut(s) 208, 2036
BssT1I CCWWGG 3 cut(s) 794, 1492, 1951
Bst2BI CACGAG 2 cut(s) 208, 2036
Bst2UI CCWGG 1 cut(s) 1401
Bst4CI ACNGT 4 cut(s) 599, 610, 664, 2003
BstACI GRCGYC 3 cut(s) 158, 246, 432
BstBI TTCGAA 1 cut(s) 403
BstC8I GCNNGC 4 cut(s) 330, 1105, 1516, 2024
BstDEI CTNAG 8 cut(s) 107, 414, 831, 1269, 1458, 1532, 1753, 2076
BstDSI CCRYGG 3 cut(s) 283, 794, 1492
BstF5I GGATG 7 cut(s) 719, 748, 781, 1024, 1153, 1252, 1654
BstFNI CGCG 4 cut(s) 398, 627, 736, 1518
BstH2I RGCGCY 2 cut(s) 249, 435
BstHHI GCGC 2 cut(s) 248, 434
BstKTI GATC 8 cut(s) 481, 877, 1130, 1288, 1885, 1904, 1984, 2166
BstMAI GTCTC 6 cut(s) 244, 312, 349, 810, 850, 1767
BstMBI GATC 8 cut(s) 478, 874, 1127, 1285, 1882, 1901, 1981, 2163
BstMCI CGRYCG 1 cut(s) 289
BstMWI GCNNNNNNNGC 4 cut(s) 26, 438, 633, 1763
BstNI CCWGG 1 cut(s) 1401
BstNSI RCATGY 2 cut(s) 793, 1825
BstSCI CCNGG 2 cut(s) 323, 1399
BstSFI CTRYAG 2 cut(s) 1111, 1734
BstUI CGCG 4 cut(s) 398, 627, 736, 1518
BstV1I GCAGC 5 cut(s) 206, 645, 1119, 1677, 1744
BstV2I GAAGAC 4 cut(s) 369, 454, 1317, 1968
BstZI CGGCCG 1 cut(s) 286
BsuI GTATCC 1 cut(s) 1736
BsuRI GGCC 3 cut(s) 29, 288, 1832
BtgI CCRYGG 3 cut(s) 283, 794, 1492
BtgZI GCGATG 2 cut(s) 16, 100
BtsCI GGATG 7 cut(s) 719, 748, 781, 1024, 1153, 1252, 1654
BtsI GCAGTG 1 cut(s) 88
BtsIMutI CAGTG 3 cut(s) 88, 606, 1593
BtuMI TCGCGA 1 cut(s) 627
BveI ACCTGC 1 cut(s) 1727
Cac8I GCNNGC 4 cut(s) 330, 1105, 1516, 2024
CfoI GCGC 2 cut(s) 248, 434
Cfr10I RCCGGY 1 cut(s) 370
Cfr13I GGNCC 4 cut(s) 551, 912, 1499, 1830
CseI GACGC 1 cut(s) 725
Csp6I GTAC 5 cut(s) 421, 527, 1543, 1973, 1992
CspAI ACCGGT 1 cut(s) 370
CviAII CATG 8 cut(s) 637, 765, 790, 795, 1082, 1493, 1822, 2060
CviQI GTAC 5 cut(s) 421, 527, 1543, 1973, 1992
DdeI CTNAG 8 cut(s) 107, 414, 831, 1269, 1458, 1532, 1753, 2076
DinI GGCGCC 2 cut(s) 247, 433
DpnI GATC 8 cut(s) 480, 876, 1129, 1287, 1884, 1903, 1983, 2165
DpnII GATC 8 cut(s) 478, 874, 1127, 1285, 1882, 1901, 1981, 2163
DraI TTTAAA 1 cut(s) 1527
EaeI YGGCCR 2 cut(s) 27, 286
EagI CGGCCG 1 cut(s) 286
EciI GGCGGA 1 cut(s) 441
EclXI CGGCCG 1 cut(s) 286
Eco130I CCWWGG 3 cut(s) 794, 1492, 1951
Eco24I GRGCYC 1 cut(s) 1163
Eco31I GGTCTC 1 cut(s) 349
Eco32I GATATC 1 cut(s) 379
Eco47I GGWCC 3 cut(s) 551, 912, 1499
Eco52I CGGCCG 1 cut(s) 286
Eco57I CTGAAG 4 cut(s) 421, 1345, 1530, 2030
Eco88I CYCGRG 1 cut(s) 302
EcoO109I RGGNCCY 1 cut(s) 1499
EcoRI GAATTC 3 cut(s) 640, 1057, 1307
EcoRII CCWGG 1 cut(s) 1399
EcoRV GATATC 1 cut(s) 379
EcoT14I CCWWGG 3 cut(s) 794, 1492, 1951
EcoT38I GRGCYC 1 cut(s) 1163
EgeI GGCGCC 2 cut(s) 247, 433
EheI GGCGCC 2 cut(s) 247, 433
ErhI CCWWGG 3 cut(s) 794, 1492, 1951
Esp3I CGTCTC 1 cut(s) 244
FaeI CATG 8 cut(s) 640, 768, 793, 798, 1085, 1496, 1825, 2063
FaqI GGGAC 1 cut(s) 202
FatI CATG 8 cut(s) 636, 764, 789, 794, 1081, 1492, 1821, 2059
FauI CCCGC 1 cut(s) 741
FbaI TGATCA 2 cut(s) 1285, 1882
FblI GTMKAC 1 cut(s) 986
Fnu4HI GCNGC 8 cut(s) 195, 257, 265, 289, 634, 1108, 1666, 1758
FokI GGATG 7 cut(s) 726, 755, 768, 1031, 1160, 1239, 1661
FriOI GRGCYC 1 cut(s) 1163
Fsp4HI GCNGC 8 cut(s) 195, 257, 265, 289, 634, 1108, 1666, 1758
FspBI CTAG 3 cut(s) 1443, 1551, 1674
GlaI GCGC 2 cut(s) 247, 433
GluI GCNGC 8 cut(s) 195, 257, 265, 289, 634, 1108, 1666, 1758
GsuI CTGGAG 3 cut(s) 522, 1746, 1809
HaeII RGCGCY 2 cut(s) 249, 435
HaeIII GGCC 3 cut(s) 29, 288, 1832
HapII CCGG 6 cut(s) 170, 243, 324, 350, 359, 371
HgaI GACGC 1 cut(s) 725
HhaI GCGC 2 cut(s) 248, 434
Hin1I GRCGYC 3 cut(s) 158, 246, 432
Hin1II CATG 8 cut(s) 640, 768, 793, 798, 1085, 1496, 1825, 2063
Hin6I GCGC 2 cut(s) 246, 432
HinP1I GCGC 2 cut(s) 246, 432
HincII GTYRAC 1 cut(s) 1579
HindII GTYRAC 1 cut(s) 1579
HpaI GTTAAC 1 cut(s) 1579
HpaII CCGG 6 cut(s) 170, 243, 324, 350, 359, 371
HphI GGTGA 6 cut(s) 217, 352, 513, 1285, 1323, 1896
Hpy166II GTNNAC 4 cut(s) 101, 551, 987, 1579
Hpy188I TCNGA 8 cut(s) 110, 319, 697, 832, 874, 1246, 2010, 2163
Hpy8I GTNNAC 4 cut(s) 101, 551, 987, 1579
Hpy99I CGWCG 1 cut(s) 319
HpyAV CCTTC 7 cut(s) 382, 445, 739, 1229, 1789, 2005, 2042
HpyCH4III ACNGT 4 cut(s) 599, 610, 664, 2003
HpyCH4IV ACGT 3 cut(s) 158, 589, 2122
HpyF10VI GCNNNNNNNGC 4 cut(s) 26, 438, 633, 1763
HpyF3I CTNAG 8 cut(s) 107, 414, 831, 1269, 1458, 1532, 1753, 2076
HpySE526I ACGT 3 cut(s) 158, 589, 2122
Hsp92I GRCGYC 3 cut(s) 158, 246, 432
Hsp92II CATG 8 cut(s) 640, 768, 793, 798, 1085, 1496, 1825, 2063
HspAI GCGC 2 cut(s) 246, 432
KasI GGCGCC 2 cut(s) 245, 431
Kpn2I TCCGGA 1 cut(s) 169
Ksp22I TGATCA 2 cut(s) 1285, 1882
KspAI GTTAAC 1 cut(s) 1579
Kzo9I GATC 8 cut(s) 478, 874, 1127, 1285, 1882, 1901, 1981, 2163
LmnI GCTCC 4 cut(s) 202, 512, 1238, 1790
Lsp1109I GCAGC 5 cut(s) 206, 645, 1119, 1677, 1744
LweI GCATC 5 cut(s) 102, 574, 733, 1009, 1250
MaeI CTAG 3 cut(s) 1443, 1551, 1674
MaeII ACGT 3 cut(s) 158, 589, 2122
MaeIII GTNAC 3 cut(s) 658, 716, 1273
MalI GATC 8 cut(s) 480, 876, 1129, 1287, 1884, 1903, 1983, 2165
MbiI CCGCTC 1 cut(s) 332
MboI GATC 8 cut(s) 478, 874, 1127, 1285, 1882, 1901, 1981, 2163
MfeI CAATTG 2 cut(s) 654, 897
MhlI GDGCHC 3 cut(s) 127, 1163, 2008
Mly113I GGCGCC 2 cut(s) 246, 432
MlyI GAGTC 5 cut(s) 105, 314, 864, 992, 1361
MmeI TCCRAC 2 cut(s) 297, 2110
MnlI CCTC 9 cut(s) 259, 302, 422, 606, 988, 1482, 1490, 1721, 1821
MroI TCCGGA 1 cut(s) 169
MspA1I CMGCKG 2 cut(s) 1508, 1668
MspI CCGG 6 cut(s) 170, 243, 324, 350, 359, 371
MspR9I CCNGG 2 cut(s) 325, 1401
MunI CAATTG 2 cut(s) 654, 897
Mva1269I GAATGC 1 cut(s) 1565
MvaI CCWGG 1 cut(s) 1401
MvnI CGCG 4 cut(s) 398, 627, 736, 1518
MwoI GCNNNNNNNGC 4 cut(s) 26, 438, 633, 1763
NarI GGCGCC 2 cut(s) 246, 432
NciI CCSGG 1 cut(s) 325
NcoI CCATGG 2 cut(s) 794, 1492
NdeII GATC 8 cut(s) 478, 874, 1127, 1285, 1882, 1901, 1981, 2163
NlaIII CATG 8 cut(s) 640, 768, 793, 798, 1085, 1496, 1825, 2063
NlaIV GGNNCC 5 cut(s) 247, 268, 433, 779, 1501
NmeAIII GCCGAG 2 cut(s) 5, 240
NmuCI GTSAC 2 cut(s) 716, 1273
NruI TCGCGA 1 cut(s) 627
NspI RCATGY 2 cut(s) 793, 1825
NspV TTCGAA 1 cut(s) 403
PaeR7I CTCGAG 1 cut(s) 302
PaqCI CACCTGC 1 cut(s) 1727
PciI ACATGT 1 cut(s) 1821
PcsI WCGNNNNNNNCGW 1 cut(s) 595
PctI GAATGC 1 cut(s) 1565
PfeI GAWTC 8 cut(s) 278, 320, 515, 692, 890, 964, 1688, 1850
PflMI CCANNNNNTGG 2 cut(s) 545, 1957
PinAI ACCGGT 1 cut(s) 370
PkrI GCNGC 8 cut(s) 196, 258, 266, 290, 635, 1109, 1667, 1759
PleI GAGTC 5 cut(s) 105, 313, 863, 991, 1360
PluTI GGCGCC 2 cut(s) 249, 435
PpsI GAGTC 5 cut(s) 105, 313, 863, 991, 1360
PpuMI RGGWCCY 1 cut(s) 1499
PscI ACATGT 1 cut(s) 1821
PshBI ATTAAT 2 cut(s) 1218, 1376
PsiI TTATAA 1 cut(s) 474
Psp5II RGGWCCY 1 cut(s) 1499
Psp6I CCWGG 1 cut(s) 1399
PspGI CCWGG 1 cut(s) 1399
PspN4I GGNNCC 5 cut(s) 247, 268, 433, 779, 1501
PspPI GGNCC 4 cut(s) 551, 912, 1499, 1830
PspPPI RGGWCCY 1 cut(s) 1499
PspXI VCTCGAGB 1 cut(s) 302
PstI CTGCAG 1 cut(s) 1738
PvuII CAGCTG 2 cut(s) 1508, 1668
RruI TCGCGA 1 cut(s) 627
RsaI GTAC 5 cut(s) 422, 528, 1544, 1974, 1993
RsaNI GTAC 5 cut(s) 421, 527, 1543, 1973, 1992
SatI GCNGC 8 cut(s) 195, 257, 265, 289, 634, 1108, 1666, 1758
Sau3AI GATC 8 cut(s) 478, 874, 1127, 1285, 1882, 1901, 1981, 2163
Sau96I GGNCC 4 cut(s) 551, 912, 1499, 1830
ScaI AGTACT 1 cut(s) 1544
SchI GAGTC 5 cut(s) 105, 314, 864, 992, 1361
ScrFI CCNGG 2 cut(s) 325, 1401
SduI GDGCHC 3 cut(s) 127, 1163, 2008
SfaNI GCATC 5 cut(s) 102, 574, 733, 1009, 1250
SfcI CTRYAG 2 cut(s) 1111, 1734
SfoI GGCGCC 2 cut(s) 247, 433
Sfr274I CTCGAG 1 cut(s) 302
SfuI TTCGAA 1 cut(s) 403
SinI GGWCC 3 cut(s) 551, 912, 1499
SlaI CTCGAG 1 cut(s) 302
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
SpeI ACTAGT 1 cut(s) 1550
SspDI GGCGCC 2 cut(s) 245, 431
SspI AATATT 2 cut(s) 1180, 1204
SspMI CTAG 3 cut(s) 1443, 1551, 1674
StyD4I CCNGG 2 cut(s) 323, 1399
StyI CCWWGG 3 cut(s) 794, 1492, 1951
TaaI ACNGT 4 cut(s) 599, 610, 664, 2003
TaiI ACGT 3 cut(s) 161, 592, 2125
TaqI TCGA 3 cut(s) 303, 403, 1771
TatI WGTACW 1 cut(s) 1542
TauI GCSGC 3 cut(s) 259, 267, 291
TfiI GAWTC 8 cut(s) 278, 320, 515, 692, 890, 964, 1688, 1850
TscAI CASTG 3 cut(s) 95, 613, 1600
TseFI GTSAC 2 cut(s) 716, 1273
TseI GCWGC 5 cut(s) 194, 633, 1107, 1665, 1757
Tsp45I GTSAC 2 cut(s) 716, 1273
TspDTI ATGAA 7 cut(s) 17, 653, 925, 1080, 1098, 1241, 2061
TspGWI ACGGA 3 cut(s) 303, 1205, 1245
TspRI CASTG 3 cut(s) 95, 613, 1600
Van91I CCANNNNNTGG 2 cut(s) 545, 1957
VpaK11BI GGWCC 3 cut(s) 551, 912, 1499
VspI ATTAAT 2 cut(s) 1218, 1376
XapI RAATTY 8 cut(s) 640, 1025, 1057, 1067, 1307, 1521, 1844, 1925
XceI RCATGY 2 cut(s) 793, 1825
XhoI CTCGAG 1 cut(s) 302
XmiI GTMKAC 1 cut(s) 986
XspI CTAG 3 cut(s) 1443, 1551, 1674
ZraI GACGTC 1 cut(s) 159
ZrmI AGTACT 1 cut(s) 1544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.