FvH4_5g12491

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000255
Physical Location & Seq
Forward (+)
7051290 .. 7053854
2565 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g12491.t1

Sequence Viewer

Length: 1965 bp
ATGGCTCCTCCTAGTCTTCTTGGCCCTCCACAGGTCACTAATCCCGTCCCATCGCCCCAACCCGACTCCACCGCAAGACCTCCGATGGGCTTGACCGAGAATTGCTCCCCAACCTTCTTAACCTCCGGCAACCCCTGCCTTGATCTCTTCTTCAAAGTCGTACCGGACACTCCGGCGTCCTATCTCAACAAACAGCTCCCTCTCGCCTGGGCCTACAATCCCCTCACTACCCTAAAACTCATCTGCAACCTCAGAAGCGTACGTGGCACAGGGAAATCCGATAAAGAAGGGTTCTACACTGCGGCGCTCTGGCTCCACAAGAACCACCCCAAAACCCTAGCGTCGAACGCGGCGTCTTTCGCGGAGTTCGGTTACTTCAAAGACTTGCCGGAGATTTTGTACCGCCTTCTAGAAGGCCAGGACGTGAGGGCGGAGCAAAAGATGGAGTGGCGGCGTCGGAAAGGGACTATCCGGAGGCTGGTAGGAAGACGTGGCACTGGACGCCGGAGGATCGTCAGACGTGGCTTGAAGACATCAAAACAGGCCGGCGGGAAAGCCAAAACCGCCGCCGCCAGCCGGCAGAAGTTGCCGAAAGAAGTAAGGGAGAAGATGGCGGCGGAGAAGCGGAGACTGGAGAAGGAGAAAGTGAGCGCGGCGAGGCTGGAGAAGAAGATTGCAATGGCTAAGAAGGCGGTGGCTAGATACCAGCGTGACCCGGATTACCGGTTCTTTCATGACCGGGTTTCGGATCTTTTCGCCGAGTGCCTGAAAGCTGATATGGAGAATCTCAAGAACAAGGAGACTAACAAGATAGGCCTGGCGGCGAAGTGGTGTCCTTCGCTTGATTCTTCGTTTGATCGAGCCACATTGCTCTGTGAAAGCATTGCGAGGAAGATTTTCACACGAGAATCGTATCGGGAATATGAAGGAGTTGAGGATGAACATTATGCTTATAGAGTCCGAGACCGGCTGAGGAAGGAGGTGCTGGTGCCGTTGAGGAAGGTGCTGCAGCTGCCGGAGATTTATATGGGTGCCAACAAGTGGGGGGAGATACCCTACAAGAGAGTGGCCTCAGTCGCCATGAAGCTTTACAAGGGGAAATTTTTGAAGCACGATGAGGAGAGGTTTAAGAAGTATTTGGAGCATGTGAAGGCCGGGAAGTCCACCATTGCTGCCGGTGCGCTGCTTCCGCATGAGATCATAGCCTCTCTGAATGATGAGGATGGTGGGGAAGTGGGTGAGCTTCAGTGGAATAGAATGGTGGAGGATCTAGTGGAGCTTGGGAAACTGAGGAACTGCGTGGCGGTGTGTGACGTGTCCGGAAGCATGGATGGGACTCCTATGGAGGTGTGTGTGGCATTGGGGTTGTTGGTGTCTGAACTGAGCGAAGAGCCATGGAAAGGAAAGGTGATCACTTTCAGTCAGAACCCTCAGATGCATTTGATTCAAGGGGACAATCTAAAATCCAAGTGTGAGTTTGTGGAGAATATGGAATGGGGGATGAACACTGATTTTCAAAAGGTGTTTGATTTGATTCTCCAAGTAGCTGTGAATGGAAAATTGAAGCCGGAGCAGATGATAAAGAGGGTGTTTGTGTTCAGTGACATGGAGTTTGATGAGGCTTCGGGGTATAGGAGTAGTTATGGATATAGATATTCTAGCTATTCTGAAAGCGAGGATGATGAGTCTAAAAACGGATGGGAGACTGATTATGAGGTGATACAGAGAAAGTTTGAAGAGAAGGGGTATGGTGATGCGGTGCCTCAGATTGTGTTTTGGAATCTGAGGGACTCACAGTCTGTACCGGTGACTGCAACAACACCAGGGGTGGCTCTGTTGAGCGGTTTCTCCAAGAATGCCCTCAAACTGTTCTTGGATGATGACATTTCCAAAATTCAACCAGACCTTGTCATGGAAGCAGCCATCTCCGGGGAGGAGTATCAGAAGCTGGTTGTGGTTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

655

Amino Acids

74.17

Weight (kDa)

8.93

Isoelectric Point (pI)

40.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2828 PF11443 32 - 430 5.4e-136 Domain of unknown function (DUF2828)
DUF7788 PF25043 432 - 632 2.2e-81 Domain of unknown function (DUF7788)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000255)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24780 AT5G13210 AT5G43390 AT5G43400
fragaria_vesca FvH4_4g23010 FvH4_5g12462 FvH4_5g12490 FvH4_5g12491 FvH4_5g20030 FvH4_5g20031 FvH4_5g21510 FvH4_6g30070
malus_domestica MD00G1095100.v1.1 MD05G1186800.v1.1 MD05G1259500.v1.1 MD05G1260200.v1.1 MD06G1159400.v1.1 MD06G1159500.v1.1 MD06G1162500.v1.1 MD06G1164600.v1.1 MD06G1187800.v1.1 MD06G1212600.v1.1 MD06G1212700.v1.1 MD13G1126800.v1.1 MD14G1168300.v1.1 MD14G1206600.v1.1 MD14G1223900.v1.1 MD14G1230800.v1.1 MD15G1240300.v1.1 MD15G1416800.v1.1 MD16G1127300.v1.1
prunus_persica Prupe.1G218700_v2.0.a1 Prupe.4G045100_v2.0.a1 Prupe.5G159300_v2.0.a1 Prupe.5G172200_v2.0.a1 Prupe.5G172300_v2.0.a1 Prupe.5G173200_v2.0.a1 Prupe.5G173300_v2.0.a1 Prupe.5G173400_v2.0.a1 Prupe.5G219000_v2.0.a1 Prupe.5G219100_v2.0.a1 Prupe.5G219200_v2.0.a1
pyrus_communis pycom05g24390 pycom06g19020 pycom10g15060 pycom10g24800 pycom13g11010 pycom16g10800 pycom16g10810 pycom17g22350 pycom17g22360
rosa_chinensis RchiOBHm_Chr2g0136111 RchiOBHm_Chr4g0429291 RchiOBHm_Chr7g0184981 RchiOBHm_Chr7g0184991 RchiOBHm_Chr7g0185001 RchiOBHm_Chr7g0204841
rosa_laevigata RLG00000003427 RLG00000004941 RLG00000004943 RLG00000004944 RLG00000004945 RLG00000004946 RLG00000007084 RLG00000013701 RLG00000019505 RLG00000019506
rosa_multiflora Rmu_co8294559.1_g000001 Rmu_co8375195.1_g000001 Rmu_co8450783.1_g000001 Rmu_co8519567.1_g000001 Rmu_sc0000054.1_g000008 Rmu_sc0001689.1_g000017 Rmu_sc0001801.1_g000027 Rmu_sc0002070.1_g000010 Rmu_sc0002180.1_g000022 Rmu_sc0002986.1_g000018 Rmu_sc0003221.1_g000027 Rmu_sc0005629.1_g000018 Rmu_sc0009898.1_g000002 Rmu_sc0018428.1_g000003 Rmu_sc0018428.1_g000005 Rmu_sc0022816.1_g000001 Rmu_sc0041781.1_g000001 Rmu_ssc0000289.1_g000007 Rmu_ssc0000289.1_g000008 Rmu_ssc0000289.1_g000010
rosa_roxburghii Rroxscaffold_2G00108460 Rroxscaffold_3G00252720 Rroxscaffold_3G00269290 Rroxscaffold_5G00349680 Rroxscaffold_5G00370900 Rroxscaffold_6G00415020
rosa_rugosa Rorug01G0043400 Rorug02G0328200 Rorug04G0234800 Rorug06G0465600 Rorug06G0465800 Rorug06G0465900 Rorug06G0466000 Rorug06G0466100 Rorug07G0086200 Rorug07G0086200
rosa_samantha Rh2BG386700 Rh2CG365900 Rh2DG402800 Rh4AG290400 Rh4BG296100 Rh4CG311900 Rh4DG293300 Rh7AG077000 Rh7AG077100 Rh7AG077200 Rh7AG216600 Rh7BG070200 Rh7BG070300 Rh7BG070400 Rh7BG213700 Rh7CG071400 Rh7CG071600 Rh7CG071700 Rh7CG071900 Rh7CG230100 Rh7DG070800 Rh7DG070900 Rh7DG224200 Rh7DG440100
rosa_wichuraiana Rw2G030970 Rw4G025150 Rw7G005840 Rw7G005850 Rw7G005860 Rw7G018770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 988, 1031, 1759
AccB7I CCANNNNNTGG 1 cut(s) 1041
AccBSI CCGCTC 1 cut(s) 1842
AccII CGCG 3 cut(s) 350, 362, 653
AccIII TCCGGA 2 cut(s) 471, 1319
AclWI GGATC 3 cut(s) 518, 756, 1275
AcsI RAATTY 2 cut(s) 1100, 1893
AcuI CTGAAG 1 cut(s) 1229
AcyI GRCGYC 4 cut(s) 176, 353, 454, 502
AfaI GTAC 4 cut(s) 162, 261, 401, 1803
AfiI CCNNNNNNNGG 9 cut(s) 31, 86, 478, 576, 745, 1041, 1400, 1912, 1929
AflIII ACRYGT 1 cut(s) 1314
AgeI ACCGGT 2 cut(s) 723, 1804
AgsI TTSAA 9 cut(s) 154, 379, 529, 1108, 1448, 1517, 1564, 1736, 1898
AhdI GACNNNNNGTC 1 cut(s) 1795
AjiI CACGTC 4 cut(s) 424, 491, 521, 1315
AjnI CCWGG 4 cut(s) 206, 417, 816, 1822
AloI GAACNNNNNNTCC 2 cut(s) 710, 742
AluBI AGCT 9 cut(s) 196, 773, 1012, 1087, 1243, 1279, 1547, 1662, 1948
AluI AGCT 9 cut(s) 196, 773, 1012, 1087, 1243, 1279, 1547, 1662, 1948
Alw26I GTCTC 4 cut(s) 622, 794, 957, 1697
AlwI GGATC 3 cut(s) 518, 756, 1275
AlwNI CAGNNNCTG 1 cut(s) 1948
Aor13HI TCCGGA 2 cut(s) 471, 1319
AoxI GGCC 7 cut(s) 22, 210, 415, 543, 814, 1068, 1152
ApeKI GCWGC 6 cut(s) 1006, 1009, 1012, 1172, 1183, 1919
ApoI RAATTY 2 cut(s) 1100, 1893
ArsI GACNNNNNNTTYG 4 cut(s) 338, 370, 1595, 1627
AsiGI ACCGGT 2 cut(s) 723, 1804
Asp700I GAANNNNTTC 1 cut(s) 896
AspLEI GCGC 3 cut(s) 307, 653, 1183
AspS9I GGNCC 2 cut(s) 23, 210
AsuC2I CCSGG 4 cut(s) 716, 740, 1156, 1930
AsuHPI GGTGA 5 cut(s) 1250, 1420, 1729, 1763, 1819
BaeI ACNNNNGTAYC 2 cut(s) 694, 727
BanI GGYRCC 3 cut(s) 988, 1031, 1759
BauI CACGAG 1 cut(s) 903
BbsI GAAGAC 3 cut(s) 8, 493, 536
BbvCI CCTCAGC 1 cut(s) 971
BbvI GCAGC 6 cut(s) 993, 999, 1021, 1159, 1170, 1931
BccI CCATC 8 cut(s) 58, 79, 436, 604, 1217, 1325, 1692, 1931
BceAI ACGGC 1 cut(s) 976
BciT130I CCWGG 4 cut(s) 208, 419, 818, 1824
BclI TGATCA 1 cut(s) 1410
BcnI CCSGG 4 cut(s) 716, 740, 1156, 1930
BcoDI GTCTC 4 cut(s) 622, 794, 957, 1697
BfaI CTAG 6 cut(s) 12, 338, 410, 699, 1271, 1659
BfmI CTRYAG 1 cut(s) 1007
BfoI RGCGCY 1 cut(s) 308
Bme1390I CCNGG 8 cut(s) 208, 419, 716, 740, 818, 1156, 1824, 1930
BmeRI GACNNNNNGTC 1 cut(s) 1795
BmgBI CACGTC 4 cut(s) 424, 491, 521, 1315
BmgT120I GGNCC 2 cut(s) 23, 210
BmiI GGNNCC 5 cut(s) 6, 314, 990, 1033, 1761
BmrFI CCNGG 8 cut(s) 208, 419, 716, 740, 818, 1156, 1824, 1930
BmsI GCATC 2 cut(s) 1425, 1744
BpiI GAAGAC 3 cut(s) 8, 493, 536
BplI GAGNNNNNCTC 2 cut(s) 89, 121
BpmI CTGGAG 2 cut(s) 653, 683
Bpu10I CCTNAGC 1 cut(s) 971
BpuEI CTTGAG 1 cut(s) 773
BpuMI CCSGG 4 cut(s) 716, 740, 1156, 1930
BsaAI YACGTR 1 cut(s) 263
BsaHI GRCGYC 4 cut(s) 176, 353, 454, 502
BsaI GGTCTC 1 cut(s) 957
BsaJI CCNNGG 4 cut(s) 207, 1394, 1823, 1929
BsaWI WCCGGW 5 cut(s) 163, 471, 723, 1319, 1804
BsaXI ACNNNNNCTCC 2 cut(s) 383, 413
Bsc4I CCNNNNNNNGG 9 cut(s) 31, 86, 478, 576, 745, 1041, 1400, 1912, 1929
Bse118I RCCGGY 6 cut(s) 545, 576, 723, 966, 1175, 1804
Bse1I ACTGG 2 cut(s) 502, 636
Bse3DI GCAATG 4 cut(s) 684, 866, 882, 1167
BseAI TCCGGA 2 cut(s) 471, 1319
BseBI CCWGG 4 cut(s) 208, 419, 818, 1824
BseDI CCNNGG 4 cut(s) 207, 1394, 1823, 1929
BseGI GGATG 7 cut(s) 943, 1228, 1336, 1506, 1684, 1703, 1882
BseLI CCNNNNNNNGG 9 cut(s) 31, 86, 478, 576, 745, 1041, 1400, 1912, 1929
BseMI GCAATG 4 cut(s) 684, 866, 882, 1167
BseMII CTCAG 8 cut(s) 265, 962, 1086, 1280, 1373, 1445, 1775, 1778
BseNI ACTGG 2 cut(s) 502, 636
BseRI GAGGAG 2 cut(s) 1133, 1949
BseXI GCAGC 6 cut(s) 993, 999, 1021, 1159, 1170, 1931
Bsh1236I CGCG 3 cut(s) 350, 362, 653
BshFI GGCC 7 cut(s) 24, 212, 417, 545, 816, 1070, 1154
BshNI GGYRCC 3 cut(s) 988, 1031, 1759
BshTI ACCGGT 2 cut(s) 723, 1804
BsiWI CGTACG 1 cut(s) 259
BslFI GGGAC 5 cut(s) 32, 478, 1348, 1466, 1802
BslI CCNNNNNNNGG 9 cut(s) 31, 86, 478, 576, 745, 1041, 1400, 1912, 1929
BsmAI GTCTC 4 cut(s) 622, 794, 957, 1697
BsmFI GGGAC 5 cut(s) 32, 478, 1348, 1466, 1802
BsmI GAATGC 1 cut(s) 1861
BsnI GGCC 7 cut(s) 24, 212, 417, 545, 816, 1070, 1154
Bso31I GGTCTC 1 cut(s) 957
Bsp13I TCCGGA 2 cut(s) 471, 1319
Bsp143I GATC 7 cut(s) 142, 510, 748, 856, 1197, 1267, 1410
Bsp19I CCATGG 1 cut(s) 1394
BspANI GGCC 7 cut(s) 24, 212, 417, 545, 816, 1070, 1154
BspCNI CTCAG 8 cut(s) 264, 963, 1085, 1281, 1374, 1444, 1776, 1777
BspEI TCCGGA 2 cut(s) 471, 1319
BspFNI CGCG 3 cut(s) 350, 362, 653
BspHI TCATGA 1 cut(s) 733
BspLI GGNNCC 5 cut(s) 6, 314, 990, 1033, 1761
BspMAI CTGCAG 1 cut(s) 1011
BspPI GGATC 3 cut(s) 518, 756, 1275
BspQI GCTCTTC 1 cut(s) 1383
BspT107I GGYRCC 3 cut(s) 988, 1031, 1759
BspTNI GGTCTC 1 cut(s) 957
BsrBI CCGCTC 1 cut(s) 1842
BsrDI GCAATG 4 cut(s) 684, 866, 882, 1167
BsrFI RCCGGY 6 cut(s) 545, 576, 723, 966, 1175, 1804
BsrI ACTGG 2 cut(s) 502, 636
BssAI RCCGGY 6 cut(s) 545, 576, 723, 966, 1175, 1804
BssECI CCNNGG 4 cut(s) 207, 1394, 1823, 1929
BssMI GATC 7 cut(s) 142, 510, 748, 856, 1197, 1267, 1410
BssNI GRCGYC 4 cut(s) 176, 353, 454, 502
BssSI CACGAG 1 cut(s) 903
BssT1I CCWWGG 1 cut(s) 1394
Bst2BI CACGAG 1 cut(s) 903
Bst2UI CCWGG 4 cut(s) 208, 419, 818, 1824
Bst4CI ACNGT 2 cut(s) 1797, 1869
Bst6I CTCTTC 3 cut(s) 152, 1383, 1731
BstACI GRCGYC 4 cut(s) 176, 353, 454, 502
BstAPI GCANNNNNTGC 2 cut(s) 135, 586
BstBAI YACGTR 1 cut(s) 263
BstC8I GCNNGC 3 cut(s) 547, 574, 578
BstDEI CTNAG 9 cut(s) 251, 684, 971, 1072, 1289, 1382, 1431, 1764, 1784
BstDSI CCRYGG 1 cut(s) 1394
BstF5I GGATG 7 cut(s) 943, 1228, 1336, 1506, 1684, 1703, 1882
BstFNI CGCG 3 cut(s) 350, 362, 653
BstH2I RGCGCY 1 cut(s) 308
BstHHI GCGC 3 cut(s) 307, 653, 1183
BstKTI GATC 7 cut(s) 145, 513, 751, 859, 1200, 1270, 1413
BstMAI GTCTC 4 cut(s) 622, 794, 957, 1697
BstMBI GATC 7 cut(s) 142, 510, 748, 856, 1197, 1267, 1410
BstNI CCWGG 4 cut(s) 208, 419, 818, 1824
BstNSI RCATGY 1 cut(s) 1148
BstSCI CCNGG 8 cut(s) 206, 417, 714, 738, 816, 1154, 1822, 1928
BstSFI CTRYAG 1 cut(s) 1007
BstUI CGCG 3 cut(s) 350, 362, 653
BstV1I GCAGC 6 cut(s) 993, 999, 1021, 1159, 1170, 1931
BstV2I GAAGAC 3 cut(s) 8, 493, 536
BstX2I RGATCY 2 cut(s) 748, 1267
BstYI RGATCY 2 cut(s) 748, 1267
BsuRI GGCC 7 cut(s) 24, 212, 417, 545, 816, 1070, 1154
BtgI CCRYGG 1 cut(s) 1394
BtgZI GCGATG 1 cut(s) 36
BtrI CACGTC 4 cut(s) 424, 491, 521, 1315
BtsCI GGATG 7 cut(s) 943, 1228, 1336, 1506, 1684, 1703, 1882
BtsI GCAGTG 1 cut(s) 297
BtsIMutI CAGTG 5 cut(s) 297, 495, 1253, 1506, 1606
Cac8I GCNNGC 3 cut(s) 547, 574, 578
CaiI CAGNNNCTG 1 cut(s) 1948
CciI TCATGA 1 cut(s) 733
CfoI GCGC 3 cut(s) 307, 653, 1183
Cfr10I RCCGGY 6 cut(s) 545, 576, 723, 966, 1175, 1804
Cfr13I GGNCC 2 cut(s) 23, 210
CseI GACGC 5 cut(s) 165, 330, 342, 443, 510
Csp6I GTAC 4 cut(s) 161, 260, 400, 1802
CspAI ACCGGT 2 cut(s) 723, 1804
CviAII CATG 8 cut(s) 734, 1081, 1145, 1193, 1327, 1395, 1606, 1912
CviQI GTAC 4 cut(s) 161, 260, 400, 1802
DdeI CTNAG 9 cut(s) 251, 684, 971, 1072, 1289, 1382, 1431, 1764, 1784
DpnI GATC 7 cut(s) 144, 512, 750, 858, 1199, 1269, 1412
DpnII GATC 7 cut(s) 142, 510, 748, 856, 1197, 1267, 1410
DriI GACNNNNNGTC 1 cut(s) 1795
Eam1104I CTCTTC 3 cut(s) 152, 1383, 1731
Eam1105I GACNNNNNGTC 1 cut(s) 1795
EarI CTCTTC 3 cut(s) 152, 1383, 1731
EciI GGCGGA 2 cut(s) 446, 632
Eco130I CCWWGG 1 cut(s) 1394
Eco147I AGGCCT 1 cut(s) 816
Eco31I GGTCTC 1 cut(s) 957
Eco57I CTGAAG 1 cut(s) 1229
EcoRII CCWGG 4 cut(s) 206, 417, 816, 1822
EcoT14I CCWWGG 1 cut(s) 1394
EcoT22I ATGCAT 1 cut(s) 1440
ErhI CCWWGG 1 cut(s) 1394
FaeI CATG 8 cut(s) 737, 1084, 1148, 1196, 1330, 1398, 1609, 1915
FaqI GGGAC 5 cut(s) 32, 478, 1348, 1466, 1802
FatI CATG 8 cut(s) 733, 1080, 1144, 1192, 1326, 1394, 1605, 1911
FauI CCCGC 1 cut(s) 542
FbaI TGATCA 1 cut(s) 1410
FokI GGATG 7 cut(s) 950, 1235, 1343, 1513, 1691, 1710, 1889
FspBI CTAG 6 cut(s) 12, 338, 410, 699, 1271, 1659
GlaI GCGC 3 cut(s) 306, 652, 1182
GsuI CTGGAG 2 cut(s) 653, 683
HaeII RGCGCY 1 cut(s) 308
HaeIII GGCC 7 cut(s) 24, 212, 417, 545, 816, 1070, 1154
HgaI GACGC 5 cut(s) 165, 330, 342, 443, 510
HhaI GCGC 3 cut(s) 307, 653, 1183
Hin1I GRCGYC 4 cut(s) 176, 353, 454, 502
Hin1II CATG 8 cut(s) 737, 1084, 1148, 1196, 1330, 1398, 1609, 1915
Hin6I GCGC 3 cut(s) 305, 651, 1181
HinP1I GCGC 3 cut(s) 305, 651, 1181
HindIII AAGCTT 1 cut(s) 1085
HphI GGTGA 5 cut(s) 1250, 1420, 1729, 1763, 1819
Hpy166II GTNNAC 1 cut(s) 1164
Hpy188III TCNNGA 6 cut(s) 410, 472, 734, 790, 917, 1320
Hpy8I GTNNAC 1 cut(s) 1164
Hpy99I CGWCG 2 cut(s) 346, 459
HpyCH4III ACNGT 2 cut(s) 1797, 1869
HpyCH4IV ACGT 5 cut(s) 262, 423, 490, 520, 1314
HpyCH4V TGCA 5 cut(s) 246, 677, 1009, 1438, 1814
HpyF3I CTNAG 9 cut(s) 251, 684, 971, 1072, 1289, 1382, 1431, 1764, 1784
HpySE526I ACGT 5 cut(s) 262, 423, 490, 520, 1314
Hsp92I GRCGYC 4 cut(s) 176, 353, 454, 502
Hsp92II CATG 8 cut(s) 737, 1084, 1148, 1196, 1330, 1398, 1609, 1915
HspAI GCGC 3 cut(s) 305, 651, 1181
Kpn2I TCCGGA 2 cut(s) 471, 1319
KroI GCCGGC 2 cut(s) 545, 576
KroNI GCCGGC 2 cut(s) 547, 578
Ksp22I TGATCA 1 cut(s) 1410
Kzo9I GATC 7 cut(s) 142, 510, 748, 856, 1197, 1267, 1410
LguI GCTCTTC 1 cut(s) 1383
LmnI GCTCC 8 cut(s) 10, 110, 201, 318, 433, 1141, 1276, 1570
Lsp1109I GCAGC 6 cut(s) 993, 999, 1021, 1159, 1170, 1931
LweI GCATC 2 cut(s) 1425, 1744
MaeI CTAG 6 cut(s) 12, 338, 410, 699, 1271, 1659
MaeII ACGT 5 cut(s) 262, 423, 490, 520, 1314
MaeIII GTNAC 6 cut(s) 34, 371, 710, 1310, 1601, 1807
MalI GATC 7 cut(s) 144, 512, 750, 858, 1199, 1269, 1412
MbiI CCGCTC 1 cut(s) 1842
MboI GATC 7 cut(s) 142, 510, 748, 856, 1197, 1267, 1410
MflI RGATCY 2 cut(s) 748, 1267
MluCI AATT 4 cut(s) 100, 1100, 1559, 1893
MlyI GAGTC 5 cut(s) 59, 966, 1330, 1694, 1784
MmeI TCCRAC 1 cut(s) 437
Mph1103I ATGCAT 1 cut(s) 1440
MroI TCCGGA 2 cut(s) 471, 1319
MroNI GCCGGC 2 cut(s) 545, 576
MroXI GAANNNNTTC 1 cut(s) 896
MseI TTAA 2 cut(s) 119, 1128
MspA1I CMGCKG 1 cut(s) 1012
MspR9I CCNGG 8 cut(s) 208, 419, 716, 740, 818, 1156, 1824, 1930
Mva1269I GAATGC 1 cut(s) 1861
MvaI CCWGG 4 cut(s) 208, 419, 818, 1824
MvnI CGCG 3 cut(s) 350, 362, 653
NaeI GCCGGC 2 cut(s) 547, 578
NciI CCSGG 4 cut(s) 716, 740, 1156, 1930
NcoI CCATGG 1 cut(s) 1394
NdeII GATC 7 cut(s) 142, 510, 748, 856, 1197, 1267, 1410
NgoMIV GCCGGC 2 cut(s) 545, 576
NlaIII CATG 8 cut(s) 737, 1084, 1148, 1196, 1330, 1398, 1609, 1915
NlaIV GGNNCC 5 cut(s) 6, 314, 990, 1033, 1761
NmeAIII GCCGAG 1 cut(s) 784
NmuCI GTSAC 5 cut(s) 34, 710, 1310, 1601, 1807
NsiI ATGCAT 1 cut(s) 1440
NspI RCATGY 1 cut(s) 1148
PagI TCATGA 1 cut(s) 733
PceI AGGCCT 1 cut(s) 816
PciSI GCTCTTC 1 cut(s) 1383
PcsI WCGNNNNNNNCGW 1 cut(s) 350
PctI GAATGC 1 cut(s) 1861
PdiI GCCGGC 2 cut(s) 547, 578
PdmI GAANNNNTTC 1 cut(s) 896
PfeI GAWTC 6 cut(s) 784, 845, 908, 1444, 1534, 1780
Pfl23II CGTACG 1 cut(s) 259
PflFI GACNNNGTC 1 cut(s) 1907
PflMI CCANNNNNTGG 1 cut(s) 1041
PinAI ACCGGT 2 cut(s) 723, 1804
PleI GAGTC 5 cut(s) 59, 965, 1330, 1693, 1784
PpsI GAGTC 5 cut(s) 59, 965, 1330, 1693, 1784
Ppu21I YACGTR 1 cut(s) 263
Psp6I CCWGG 4 cut(s) 206, 417, 816, 1822
PspGI CCWGG 4 cut(s) 206, 417, 816, 1822
PspLI CGTACG 1 cut(s) 259
PspN4I GGNNCC 5 cut(s) 6, 314, 990, 1033, 1761
PspPI GGNCC 2 cut(s) 23, 210
PstI CTGCAG 1 cut(s) 1011
PstNI CAGNNNCTG 1 cut(s) 1948
PsuI RGATCY 2 cut(s) 748, 1267
PsyI GACNNNGTC 1 cut(s) 1907
PvuII CAGCTG 1 cut(s) 1012
RsaI GTAC 4 cut(s) 162, 261, 401, 1803
RsaNI GTAC 4 cut(s) 161, 260, 400, 1802
SapI GCTCTTC 1 cut(s) 1383
SaqAI TTAA 2 cut(s) 119, 1128
Sau3AI GATC 7 cut(s) 142, 510, 748, 856, 1197, 1267, 1410
Sau96I GGNCC 2 cut(s) 23, 210
SchI GAGTC 5 cut(s) 59, 966, 1330, 1694, 1784
ScrFI CCNGG 8 cut(s) 208, 419, 716, 740, 818, 1156, 1824, 1930
SfaNI GCATC 2 cut(s) 1425, 1744
SfcI CTRYAG 1 cut(s) 1007
SmlI CTYRAG 1 cut(s) 788
SmoI CTYRAG 1 cut(s) 788
Sse9I AATT 4 cut(s) 100, 1100, 1559, 1893
SseBI AGGCCT 1 cut(s) 816
SspMI CTAG 6 cut(s) 12, 338, 410, 699, 1271, 1659
StuI AGGCCT 1 cut(s) 816
StyD4I CCNGG 8 cut(s) 206, 417, 714, 738, 816, 1154, 1822, 1928
StyI CCWWGG 1 cut(s) 1394
TaaI ACNGT 2 cut(s) 1797, 1869
TaiI ACGT 5 cut(s) 265, 426, 493, 523, 1317
TaqI TCGA 2 cut(s) 344, 859
TaqII GACCGA 1 cut(s) 110
TasI AATT 4 cut(s) 100, 1100, 1559, 1893
TauI GCSGC 8 cut(s) 305, 353, 454, 569, 572, 617, 656, 824
TfiI GAWTC 6 cut(s) 784, 845, 908, 1444, 1534, 1780
Tru1I TTAA 2 cut(s) 119, 1128
Tru9I TTAA 2 cut(s) 119, 1128
TscAI CASTG 5 cut(s) 304, 502, 1253, 1513, 1606
TseFI GTSAC 5 cut(s) 34, 710, 1310, 1601, 1807
TseI GCWGC 6 cut(s) 1006, 1009, 1012, 1172, 1183, 1919
Tsp45I GTSAC 5 cut(s) 34, 710, 1310, 1601, 1807
TspDTI ATGAA 5 cut(s) 722, 939, 954, 1097, 1517
TspGWI ACGGA 1 cut(s) 1710
TspRI CASTG 5 cut(s) 304, 502, 1253, 1513, 1606
Tth111I GACNNNGTC 1 cut(s) 1907
Van91I CCANNNNNTGG 1 cut(s) 1041
XapI RAATTY 2 cut(s) 1100, 1893
XbaI TCTAGA 1 cut(s) 409
XceI RCATGY 1 cut(s) 1148
XmnI GAANNNNTTC 1 cut(s) 896
XspI CTAG 6 cut(s) 12, 338, 410, 699, 1271, 1659
Zsp2I ATGCAT 1 cut(s) 1440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.