MD14G1230800.v1.1

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000255
Physical Location & Seq
Forward (+)
31147473 .. 31147943
471 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1230800.v1.1.491

Sequence Viewer

Length: 471 bp
ATGTTAGCTCCCCCATATCATAAGCTCAAACAACTCCACCAATCCATGGTTGCCGCCAGGACGAATATAATCAGTGACCCCTCCCTTGATCTGTACTTTGGCGTCCAACCAGACAGCCTGGCCCCCGATAAGTACAAAAAACTCGATCAGCTACTACCACTGGCATGGTCCCACAATCCCCTCACCACCCTCAAGCTCATTTGTAACCTACGAGACGATTCCAACGCTCGTGGAAAATCTGATGAAAAAGCGTTTTACAGGGCGGCGCTATGGCTCCGCAAGAACCACCCCCGGGCTCTGAAACGCAATGTCGCCTCTATTGCCCGCGGCGAGTATACCAAGTCGGTCGGAACTGTGGATGACCTCGCCCAGATTCTCTACTGCATTACAAGGACCTTGAGAGGTACGAGCGTGACTTGGATTATCGCTTCTTACAATACCGGGTTACGGATCTCTTCGCAGACTATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

157

Amino Acids

17.6

Weight (kDa)

9.82

Isoelectric Point (pI)

30.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2828 PF11443 25 - 131 3.8e-24 Domain of unknown function (DUF2828)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000255)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24780 AT5G13210 AT5G43390 AT5G43400
fragaria_vesca FvH4_4g23010 FvH4_5g12462 FvH4_5g12490 FvH4_5g12491 FvH4_5g20030 FvH4_5g20031 FvH4_5g21510 FvH4_6g30070
malus_domestica MD00G1095100.v1.1 MD05G1186800.v1.1 MD05G1259500.v1.1 MD05G1260200.v1.1 MD06G1159400.v1.1 MD06G1159500.v1.1 MD06G1162500.v1.1 MD06G1164600.v1.1 MD06G1187800.v1.1 MD06G1212600.v1.1 MD06G1212700.v1.1 MD13G1126800.v1.1 MD14G1168300.v1.1 MD14G1206600.v1.1 MD14G1223900.v1.1 MD14G1230800.v1.1 MD15G1240300.v1.1 MD15G1416800.v1.1 MD16G1127300.v1.1
prunus_persica Prupe.1G218700_v2.0.a1 Prupe.4G045100_v2.0.a1 Prupe.5G159300_v2.0.a1 Prupe.5G172200_v2.0.a1 Prupe.5G172300_v2.0.a1 Prupe.5G173200_v2.0.a1 Prupe.5G173300_v2.0.a1 Prupe.5G173400_v2.0.a1 Prupe.5G219000_v2.0.a1 Prupe.5G219100_v2.0.a1 Prupe.5G219200_v2.0.a1
pyrus_communis pycom05g24390 pycom06g19020 pycom10g15060 pycom10g24800 pycom13g11010 pycom16g10800 pycom16g10810 pycom17g22350 pycom17g22360
rosa_chinensis RchiOBHm_Chr2g0136111 RchiOBHm_Chr4g0429291 RchiOBHm_Chr7g0184981 RchiOBHm_Chr7g0184991 RchiOBHm_Chr7g0185001 RchiOBHm_Chr7g0204841
rosa_laevigata RLG00000003427 RLG00000004941 RLG00000004943 RLG00000004944 RLG00000004945 RLG00000004946 RLG00000007084 RLG00000013701 RLG00000019505 RLG00000019506
rosa_multiflora Rmu_co8294559.1_g000001 Rmu_co8375195.1_g000001 Rmu_co8450783.1_g000001 Rmu_co8519567.1_g000001 Rmu_sc0000054.1_g000008 Rmu_sc0001689.1_g000017 Rmu_sc0001801.1_g000027 Rmu_sc0002070.1_g000010 Rmu_sc0002180.1_g000022 Rmu_sc0002986.1_g000018 Rmu_sc0003221.1_g000027 Rmu_sc0005629.1_g000018 Rmu_sc0009898.1_g000002 Rmu_sc0018428.1_g000003 Rmu_sc0018428.1_g000005 Rmu_sc0022816.1_g000001 Rmu_sc0041781.1_g000001 Rmu_ssc0000289.1_g000007 Rmu_ssc0000289.1_g000008 Rmu_ssc0000289.1_g000010
rosa_roxburghii Rroxscaffold_2G00108460 Rroxscaffold_3G00252720 Rroxscaffold_3G00269290 Rroxscaffold_5G00349680 Rroxscaffold_5G00370900 Rroxscaffold_6G00415020
rosa_rugosa Rorug01G0043400 Rorug02G0328200 Rorug04G0234800 Rorug06G0465600 Rorug06G0465800 Rorug06G0465900 Rorug06G0466000 Rorug06G0466100 Rorug07G0086200 Rorug07G0086200
rosa_samantha Rh2BG386700 Rh2CG365900 Rh2DG402800 Rh4AG290400 Rh4BG296100 Rh4CG311900 Rh4DG293300 Rh7AG077000 Rh7AG077100 Rh7AG077200 Rh7AG216600 Rh7BG070200 Rh7BG070300 Rh7BG070400 Rh7BG213700 Rh7CG071400 Rh7CG071600 Rh7CG071700 Rh7CG071900 Rh7CG230100 Rh7DG070800 Rh7DG070900 Rh7DG224200 Rh7DG440100
rosa_wichuraiana Rw2G030970 Rw4G025150 Rw7G005840 Rw7G005850 Rw7G005860 Rw7G018770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 46
AccI GTMKAC 1 cut(s) 335
AccII CGCG 1 cut(s) 327
AciI CCGC 5 cut(s) 54, 263, 277, 325, 327
AclWI GGATC 1 cut(s) 458
AcyI GRCGYC 1 cut(s) 102
AfaI GTAC 3 cut(s) 95, 134, 406
AfiI CCNNNNNNNGG 3 cut(s) 46, 292, 447
AjnI CCWGG 2 cut(s) 56, 117
AluBI AGCT 4 cut(s) 8, 25, 151, 196
AluI AGCT 4 cut(s) 8, 25, 151, 196
Alw26I GTCTC 1 cut(s) 207
AlwI GGATC 1 cut(s) 458
Ama87I CYCGRG 1 cut(s) 291
AoxI GGCC 1 cut(s) 120
AspLEI GCGC 1 cut(s) 268
AspS9I GGNCC 3 cut(s) 121, 168, 393
AsuC2I CCSGG 3 cut(s) 292, 293, 442
AsuHPI GGTGA 1 cut(s) 175
AvaI CYCGRG 1 cut(s) 291
AvaII GGWCC 2 cut(s) 168, 393
BaeI ACNNNNGTAYC 2 cut(s) 396, 429
BanII GRGCYC 1 cut(s) 298
BauI CACGAG 1 cut(s) 228
BciT130I CCWGG 2 cut(s) 58, 119
BcnI CCSGG 3 cut(s) 292, 293, 442
BcoDI GTCTC 1 cut(s) 207
BfoI RGCGCY 1 cut(s) 269
BisI GCNGC 3 cut(s) 54, 264, 328
BlsI GCNGC 3 cut(s) 55, 265, 329
Bme1390I CCNGG 5 cut(s) 58, 119, 292, 293, 442
Bme18I GGWCC 2 cut(s) 168, 393
BmeT110I CYCGRG 1 cut(s) 291
BmgT120I GGNCC 3 cut(s) 121, 168, 393
BmiI GGNNCC 3 cut(s) 123, 170, 275
BmrFI CCNGG 5 cut(s) 58, 119, 292, 293, 442
BpuEI CTTGAG 2 cut(s) 176, 418
BpuMI CCSGG 3 cut(s) 292, 293, 442
BsaHI GRCGYC 1 cut(s) 102
BsaJI CCNNGG 4 cut(s) 45, 290, 291, 325
Bsc4I CCNNNNNNNGG 3 cut(s) 46, 292, 447
Bse1I ACTGG 1 cut(s) 165
Bse3DI GCAATG 1 cut(s) 313
BseBI CCWGG 2 cut(s) 58, 119
BseDI CCNNGG 4 cut(s) 45, 290, 291, 325
BseGI GGATG 1 cut(s) 364
BseLI CCNNNNNNNGG 3 cut(s) 46, 292, 447
BseMI GCAATG 1 cut(s) 313
BseNI ACTGG 1 cut(s) 165
Bsh1236I CGCG 1 cut(s) 327
Bsh1285I CGRYCG 1 cut(s) 348
BshFI GGCC 1 cut(s) 122
BsiEI CGRYCG 1 cut(s) 348
BsiHKCI CYCGRG 1 cut(s) 291
BsiSI CCGG 2 cut(s) 292, 441
BslFI GGGAC 1 cut(s) 154
BslI CCNNNNNNNGG 3 cut(s) 46, 292, 447
BsmAI GTCTC 1 cut(s) 207
BsmBI CGTCTC 1 cut(s) 207
BsmFI GGGAC 1 cut(s) 154
BsnI GGCC 1 cut(s) 122
BsoBI CYCGRG 1 cut(s) 291
Bsp1286I GDGCHC 1 cut(s) 298
Bsp143I GATC 3 cut(s) 88, 145, 450
Bsp19I CCATGG 1 cut(s) 45
BspACI CCGC 5 cut(s) 54, 263, 277, 325, 327
BspANI GGCC 1 cut(s) 122
BspFNI CGCG 1 cut(s) 327
BspLI GGNNCC 3 cut(s) 123, 170, 275
BspPI GGATC 1 cut(s) 458
BsrDI GCAATG 1 cut(s) 313
BsrI ACTGG 1 cut(s) 165
BssECI CCNNGG 4 cut(s) 45, 290, 291, 325
BssMI GATC 3 cut(s) 88, 145, 450
BssNAI GTATAC 1 cut(s) 336
BssNI GRCGYC 1 cut(s) 102
BssSI CACGAG 1 cut(s) 228
BssT1I CCWWGG 1 cut(s) 45
Bst1107I GTATAC 1 cut(s) 336
Bst2BI CACGAG 1 cut(s) 228
Bst2UI CCWGG 2 cut(s) 58, 119
Bst4CI ACNGT 1 cut(s) 355
Bst6I CTCTTC 1 cut(s) 460
BstACI GRCGYC 1 cut(s) 102
BstC8I GCNNGC 1 cut(s) 325
BstDSI CCRYGG 2 cut(s) 45, 325
BstF5I GGATG 1 cut(s) 364
BstFNI CGCG 1 cut(s) 327
BstH2I RGCGCY 1 cut(s) 269
BstHHI GCGC 1 cut(s) 268
BstKTI GATC 3 cut(s) 91, 148, 453
BstMAI GTCTC 1 cut(s) 207
BstMBI GATC 3 cut(s) 88, 145, 450
BstMCI CGRYCG 1 cut(s) 348
BstMWI GCNNNNNNNGC 1 cut(s) 320
BstNI CCWGG 2 cut(s) 58, 119
BstSCI CCNGG 5 cut(s) 56, 117, 290, 291, 440
BstUI CGCG 1 cut(s) 327
BstX2I RGATCY 1 cut(s) 450
BstXI CCANNNNNNTGG 1 cut(s) 165
BstYI RGATCY 1 cut(s) 450
BstZ17I GTATAC 1 cut(s) 336
BsuRI GGCC 1 cut(s) 122
BtgI CCRYGG 2 cut(s) 45, 325
BtsCI GGATG 1 cut(s) 364
BtsIMutI CAGTG 2 cut(s) 79, 158
Cac8I GCNNGC 1 cut(s) 325
CfoI GCGC 1 cut(s) 268
Cfr13I GGNCC 3 cut(s) 121, 168, 393
Cfr42I CCGCGG 1 cut(s) 328
Cfr9I CCCGGG 1 cut(s) 291
CseI GACGC 1 cut(s) 91
Csp6I GTAC 3 cut(s) 94, 133, 405
CspCI CAANNNNNGTGG 2 cut(s) 211, 246
CviAII CATG 2 cut(s) 46, 165
CviJI RGCY 8 cut(s) 8, 25, 117, 122, 151, 196, 274, 296
CviKI_1 RGCY 8 cut(s) 8, 25, 117, 122, 151, 196, 274, 296
CviQI GTAC 3 cut(s) 94, 133, 405
DpnI GATC 3 cut(s) 90, 147, 452
DpnII GATC 3 cut(s) 88, 145, 450
Eam1104I CTCTTC 1 cut(s) 460
EarI CTCTTC 1 cut(s) 460
Eco130I CCWWGG 1 cut(s) 45
Eco24I GRGCYC 1 cut(s) 298
Eco47I GGWCC 2 cut(s) 168, 393
Eco88I CYCGRG 1 cut(s) 291
EcoO109I RGGNCCY 1 cut(s) 393
EcoRII CCWGG 2 cut(s) 56, 117
EcoT14I CCWWGG 1 cut(s) 45
EcoT38I GRGCYC 1 cut(s) 298
ErhI CCWWGG 1 cut(s) 45
Esp3I CGTCTC 1 cut(s) 207
FaeI CATG 2 cut(s) 49, 168
FaiI YATR 7 cut(s) 16, 21, 47, 68, 166, 271, 336
FaqI GGGAC 1 cut(s) 154
FatI CATG 2 cut(s) 45, 164
FauI CCCGC 1 cut(s) 332
FblI GTMKAC 1 cut(s) 335
Fnu4HI GCNGC 3 cut(s) 54, 264, 328
FokI GGATG 1 cut(s) 371
FriOI GRGCYC 1 cut(s) 298
Fsp4HI GCNGC 3 cut(s) 54, 264, 328
GlaI GCGC 1 cut(s) 267
GluI GCNGC 3 cut(s) 54, 264, 328
HaeII RGCGCY 1 cut(s) 269
HaeIII GGCC 1 cut(s) 122
HapII CCGG 2 cut(s) 292, 441
HgaI GACGC 1 cut(s) 91
HhaI GCGC 1 cut(s) 268
Hin1I GRCGYC 1 cut(s) 102
Hin1II CATG 2 cut(s) 49, 168
Hin6I GCGC 1 cut(s) 266
HinP1I GCGC 1 cut(s) 266
HinfI GANTC 2 cut(s) 218, 373
HpaII CCGG 2 cut(s) 292, 441
HphI GGTGA 1 cut(s) 175
Hpy166II GTNNAC 1 cut(s) 336
Hpy188I TCNGA 3 cut(s) 241, 300, 350
Hpy8I GTNNAC 1 cut(s) 336
HpyCH4III ACNGT 1 cut(s) 355
HpyCH4V TGCA 1 cut(s) 384
HpyF10VI GCNNNNNNNGC 1 cut(s) 320
Hsp92I GRCGYC 1 cut(s) 102
Hsp92II CATG 2 cut(s) 49, 168
HspAI GCGC 1 cut(s) 266
KspI CCGCGG 1 cut(s) 328
Kzo9I GATC 3 cut(s) 88, 145, 450
LmnI GCTCC 2 cut(s) 13, 279
MaeIII GTNAC 4 cut(s) 74, 203, 412, 444
MalI GATC 3 cut(s) 90, 147, 452
MboI GATC 3 cut(s) 88, 145, 450
MboII GAAGA 1 cut(s) 447
MflI RGATCY 1 cut(s) 450
MhlI GDGCHC 1 cut(s) 298
MmeI TCCRAC 3 cut(s) 130, 246, 328
MnlI CCTC 6 cut(s) 91, 191, 200, 325, 374, 395
MseI TTAA 1 cut(s) 469
MslI CAYNNNNRTG 1 cut(s) 163
MspA1I CMGCKG 1 cut(s) 327
MspI CCGG 2 cut(s) 292, 441
MspR9I CCNGG 5 cut(s) 58, 119, 292, 293, 442
MvaI CCWGG 2 cut(s) 58, 119
MvnI CGCG 1 cut(s) 327
MwoI GCNNNNNNNGC 1 cut(s) 320
NciI CCSGG 3 cut(s) 292, 293, 442
NcoI CCATGG 1 cut(s) 45
NdeII GATC 3 cut(s) 88, 145, 450
NlaIII CATG 2 cut(s) 49, 168
NlaIV GGNNCC 3 cut(s) 123, 170, 275
NmuCI GTSAC 2 cut(s) 74, 412
PfeI GAWTC 2 cut(s) 218, 373
PflMI CCANNNNNTGG 1 cut(s) 46
PkrI GCNGC 3 cut(s) 55, 265, 329
PpuMI RGGWCCY 1 cut(s) 393
Psp5II RGGWCCY 1 cut(s) 393
Psp6I CCWGG 2 cut(s) 56, 117
PspGI CCWGG 2 cut(s) 56, 117
PspN4I GGNNCC 3 cut(s) 123, 170, 275
PspPI GGNCC 3 cut(s) 121, 168, 393
PspPPI RGGWCCY 1 cut(s) 393
PsuI RGATCY 1 cut(s) 450
RsaI GTAC 3 cut(s) 95, 134, 406
RsaNI GTAC 3 cut(s) 94, 133, 405
RseI CAYNNNNRTG 1 cut(s) 163
SacII CCGCGG 1 cut(s) 328
SaqAI TTAA 1 cut(s) 469
SatI GCNGC 3 cut(s) 54, 264, 328
Sau3AI GATC 3 cut(s) 88, 145, 450
Sau96I GGNCC 3 cut(s) 121, 168, 393
ScrFI CCNGG 5 cut(s) 58, 119, 292, 293, 442
SduI GDGCHC 1 cut(s) 298
SetI ASST 8 cut(s) 10, 27, 153, 198, 210, 366, 398, 406
Sfr303I CCGCGG 1 cut(s) 328
SgrBI CCGCGG 1 cut(s) 328
SinI GGWCC 2 cut(s) 168, 393
SmaI CCCGGG 1 cut(s) 293
SmiMI CAYNNNNRTG 1 cut(s) 163
SmlI CTYRAG 2 cut(s) 191, 397
SmoI CTYRAG 2 cut(s) 191, 397
SsiI CCGC 5 cut(s) 54, 263, 277, 325, 327
StyD4I CCNGG 5 cut(s) 56, 117, 290, 291, 440
StyI CCWWGG 1 cut(s) 45
TaaI ACNGT 1 cut(s) 355
TaqI TCGA 1 cut(s) 144
TaqII GACCGA 1 cut(s) 334
TatI WGTACW 2 cut(s) 93, 132
TauI GCSGC 3 cut(s) 56, 266, 330
TfiI GAWTC 2 cut(s) 218, 373
Tru1I TTAA 1 cut(s) 469
Tru9I TTAA 1 cut(s) 469
TscAI CASTG 2 cut(s) 79, 165
TseFI GTSAC 2 cut(s) 74, 412
Tsp45I GTSAC 2 cut(s) 74, 412
TspDTI ATGAA 1 cut(s) 258
TspGWI ACGGA 1 cut(s) 463
TspMI CCCGGG 1 cut(s) 291
TspRI CASTG 2 cut(s) 79, 165
Van91I CCANNNNNTGG 1 cut(s) 46
VpaK11BI GGWCC 2 cut(s) 168, 393
XmaI CCCGGG 1 cut(s) 291
XmiI GTMKAC 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.