RchiOBHm_Chr7g0185001

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000255
Physical Location & Seq
Reverse (-)
5278501 .. 5281106
2606 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16507

Sequence Viewer

Length: 2322 bp
ATGAAGCCACAAATCCTCTCAATTCTCAAAGGCGCCAAACCAAACCAAAACCATGTCAACTTGGCAAGAACCCAAACTCCTACTCTTCTTCTCGGTCCTCCAGAGATCGGAACAGTACCCACCACCAATGCTCCACCACAAGTCAATTACGGCGACCCCTTCATGGATCTCATGGTCTCCAATTACAACAACACCACGTCAACTTCCCCCCGACCCCCAATGGGTTTGACCGAGAACAGTTCGCCTACTTTTCTCACCTCCGGCAACCCCTGCCTTGATTTTTTCTTCCACGTGGTGCCTGACACCAAGCCTGAATATTTCAACCAACAGCTCCCATTGGCTTGGTCCAACGATGCCGTAACCGCTCTCAAGCTCATCTGCAACCTGCGAGGTGTACGTGGCACCGGAAAGTGTGACAAAGAAGGGTTCTACACAGCAGCGTATTGGCTCTACAACCACCACCCTAAAACCCTAGCTTACAATGTAGCGTCGGTTGCCGCTTTTGGGTATTTCAAAGATCTGCCGGAGATTCTATACCGCCTTCTAGAAGGCCAAGACATCAGGAAGACCCAAAAGGAAGAGTGGCAAAGGATCAAGCGCTATTTCTATAAGGGGCCGGGTAAGGTAGACGGGTTAAGGTCACTAAAGCTTGGGCATTTAATGGGTCCGGCGGATCATCCACCTAGAGATGTTAAACTGCTGAGGCTTTGGGAGAAACTCATCGCCGTCAAATCTAACAAGAAAAAACTCGAGAAGGAGGAGTATAAAACTGTTCAAGCAGAATGGGCAGCGGATAGAGACGAACTGAAAAGTGATGAAGCTAGAGAACTGATGCATCAAAAGATCATAGACAAGACCAAGAATGCCGTGGCTAGGTATGAGCAAGACTCGGATTTGAAGTTCCTATACGAACAAATTTGGGATGTGTTTGGGGATTGCTTGAAGTCCATGGAGGCGGACACGGAGGCTGAGAGGAACGAGATGATCCGATTGAAAAAAGAAAAAGTCAGGGAATTGAGGTATGAAAGAATCTATGACATGGCCAGGAAGGTGGTTTCCAAGTATCAGCAAGACTCCAATTTTCAGTTGCTTCACGAGCAGATTTCTGATGTTTTCGCCGAGTGCTTGAGATCTGACATGGAATGCTTGAAGAAGCACCAGAGAAACAAGATAAGCCTCGCAGCGAAATGGTGCCCTTCCCTCGACTCCTCCTTCGACAAGGCCACTTTGCTCTGCGAAAGCATTGCCAGGAAGGTTTTCCCGCGTGAATTGTACCCGGAGTATCAGGGACTTGAGGAGGCACACTATGCTTACAGAGTGAGAGACCGGCTGAGGAAGGAGGTTTATGTGCCGTTGAGGAGGGCATTGGAATTGCCGGAACTTTATATGACTGACAGAGAGTGGAATTCACTTCCTTACAACAGGGTGGCCTCTGTGGCAATGAAGCTCTACAAACATAGGTTCATTGAGAAGGACTGTGAGAGGTTTGAAAAATATTTGGCGGAAGTGAAAGAAGGTGTGGCCAAGATTGCGGCCGGTGCTCTGCTTCCACATGAGATCATAGCCTCTCTGAATGATGGGGATGGAGGGCAAGTGGCTGAGCTTCAGTGGAAGAGAATGGTGGAGGACCTGGTGGAGCTTGGCAAGCTGAGGAATTGCTTGGCTGTGTGTGACGTGTCCGGAAGCATGGAAGGGACTCCTATGGAGGTGTGTGTGGCTTTGGGTCTGTTGGTGTCGGAATTGATTGAAGAGCCATGGAAAGGAAAGGTGATCACTTTCAGTCAGAACCCTCAGTTGCATTTGATTCAAGGCGAGGATCTTAGCTCCAAGTGCGAGTTTGTGAGGAGGATGGAATGGGGGATGAACACTGATTTTCAAAAGGTGTTTGATCTGATTCTCCAAGTGGCTGTGAATGGGAACTTGAAGCCGGAGCAAATGATAAAGAGGGTGTTTGTGTTTAGCGACATGGAGTTTGATCAGGCTTCAGGGCATATATCACAGTTCTATGATAGTTATTGGGGGTATCATCATTGTGGTCAAAGGGAGAAAAGCAATAGTTGGGAGACTGATTATGAGGTGATACAGAGGAAGTTTAGGGAGAAAGGGTACGGGGATGCAGTGCCACAGATAGTGTTTTGGAATCTGAGGGACTCTAGGTCTACGCCGGTAACGGAGACACAAAATGGAGTGGCGCTGGTGAGTGGCTTCTCCAAGAATCTACTCAAGCTGTTCTTGGACAATGATGGCCAAATTCGGCCGGACCTTATCATGGAAAAAGCTATCTCTGGGAAAGAGTATCAGCATCTAGTTGTTGTGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

773

Amino Acids

89.19

Weight (kDa)

6.81

Isoelectric Point (pI)

34.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2828 PF11443 77 - 316 2.5e-48 Domain of unknown function (DUF2828)
DUF2828 PF11443 322 - 550 5.9e-82 Domain of unknown function (DUF2828)
DUF7788 PF25043 552 - 751 1.4e-82 Domain of unknown function (DUF7788)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000255)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24780 AT5G13210 AT5G43390 AT5G43400
fragaria_vesca FvH4_4g23010 FvH4_5g12462 FvH4_5g12490 FvH4_5g12491 FvH4_5g20030 FvH4_5g20031 FvH4_5g21510 FvH4_6g30070
malus_domestica MD00G1095100.v1.1 MD05G1186800.v1.1 MD05G1259500.v1.1 MD05G1260200.v1.1 MD06G1159400.v1.1 MD06G1159500.v1.1 MD06G1162500.v1.1 MD06G1164600.v1.1 MD06G1187800.v1.1 MD06G1212600.v1.1 MD06G1212700.v1.1 MD13G1126800.v1.1 MD14G1168300.v1.1 MD14G1206600.v1.1 MD14G1223900.v1.1 MD14G1230800.v1.1 MD15G1240300.v1.1 MD15G1416800.v1.1 MD16G1127300.v1.1
prunus_persica Prupe.1G218700_v2.0.a1 Prupe.4G045100_v2.0.a1 Prupe.5G159300_v2.0.a1 Prupe.5G172200_v2.0.a1 Prupe.5G172300_v2.0.a1 Prupe.5G173200_v2.0.a1 Prupe.5G173300_v2.0.a1 Prupe.5G173400_v2.0.a1 Prupe.5G219000_v2.0.a1 Prupe.5G219100_v2.0.a1 Prupe.5G219200_v2.0.a1
pyrus_communis pycom05g24390 pycom06g19020 pycom10g15060 pycom10g24800 pycom13g11010 pycom16g10800 pycom16g10810 pycom17g22350 pycom17g22360
rosa_chinensis RchiOBHm_Chr2g0136111 RchiOBHm_Chr4g0429291 RchiOBHm_Chr7g0184981 RchiOBHm_Chr7g0184991 RchiOBHm_Chr7g0185001 RchiOBHm_Chr7g0204841
rosa_laevigata RLG00000003427 RLG00000004941 RLG00000004943 RLG00000004944 RLG00000004945 RLG00000004946 RLG00000007084 RLG00000013701 RLG00000019505 RLG00000019506
rosa_multiflora Rmu_co8294559.1_g000001 Rmu_co8375195.1_g000001 Rmu_co8450783.1_g000001 Rmu_co8519567.1_g000001 Rmu_sc0000054.1_g000008 Rmu_sc0001689.1_g000017 Rmu_sc0001801.1_g000027 Rmu_sc0002070.1_g000010 Rmu_sc0002180.1_g000022 Rmu_sc0002986.1_g000018 Rmu_sc0003221.1_g000027 Rmu_sc0005629.1_g000018 Rmu_sc0009898.1_g000002 Rmu_sc0018428.1_g000003 Rmu_sc0018428.1_g000005 Rmu_sc0022816.1_g000001 Rmu_sc0041781.1_g000001 Rmu_ssc0000289.1_g000007 Rmu_ssc0000289.1_g000008 Rmu_ssc0000289.1_g000010
rosa_roxburghii Rroxscaffold_2G00108460 Rroxscaffold_3G00252720 Rroxscaffold_3G00269290 Rroxscaffold_5G00349680 Rroxscaffold_5G00370900 Rroxscaffold_6G00415020
rosa_rugosa Rorug01G0043400 Rorug02G0328200 Rorug04G0234800 Rorug06G0465600 Rorug06G0465800 Rorug06G0465900 Rorug06G0466000 Rorug06G0466100 Rorug07G0086200 Rorug07G0086200
rosa_samantha Rh2BG386700 Rh2CG365900 Rh2DG402800 Rh4AG290400 Rh4BG296100 Rh4CG311900 Rh4DG293300 Rh7AG077000 Rh7AG077100 Rh7AG077200 Rh7AG216600 Rh7BG070200 Rh7BG070300 Rh7BG070400 Rh7BG213700 Rh7CG071400 Rh7CG071600 Rh7CG071700 Rh7CG071900 Rh7CG230100 Rh7DG070800 Rh7DG070900 Rh7DG224200 Rh7DG440100
rosa_wichuraiana Rw2G030970 Rw4G025150 Rw7G005840 Rw7G005850 Rw7G005860 Rw7G018770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 393
AccB1I GGYRCC 4 cut(s) 32, 295, 401, 1191
AccBSI CCGCTC 1 cut(s) 365
AccI GTMKAC 2 cut(s) 627, 2159
AccII CGCG 1 cut(s) 1264
AccIII TCCGGA 1 cut(s) 1679
AciI CCGC 9 cut(s) 363, 498, 538, 671, 791, 956, 1262, 1502, 1532
AclWI GGATC 5 cut(s) 174, 599, 681, 979, 1824
AcoI YGGCCR 5 cut(s) 1041, 1521, 1533, 2245, 2255
AcsI RAATTY 3 cut(s) 915, 1405, 2250
AcuI CTGAAG 2 cut(s) 1589, 1968
AcvI CACGTG 1 cut(s) 292
AcyI GRCGYC 1 cut(s) 33
AdeI CACNNNGTG 1 cut(s) 295
AfaI GTAC 4 cut(s) 117, 396, 1274, 2108
AfeI AGCGCT 1 cut(s) 599
AfiI CCNNNNNNNGG 7 cut(s) 107, 163, 221, 504, 873, 1760, 2269
AflIII ACRYGT 1 cut(s) 1674
AhdI GACNNNNNGTC 1 cut(s) 2155
AjiI CACGTC 2 cut(s) 198, 1675
AjnI CCWGG 3 cut(s) 1043, 1247, 1629
AloI GAACNNNNNNTCC 4 cut(s) 61, 93, 884, 916
Alw21I GWGCWC 1 cut(s) 1543
Alw26I GTCTC 5 cut(s) 181, 792, 1317, 2057, 2168
AlwI GGATC 5 cut(s) 174, 599, 681, 979, 1824
Ama87I CYCGRG 1 cut(s) 749
Aor13HI TCCGGA 1 cut(s) 1679
Aor51HI AGCGCT 1 cut(s) 599
AoxI GGCC 9 cut(s) 550, 614, 1041, 1221, 1428, 1521, 1533, 2245, 2255
ApeKI GCWGC 3 cut(s) 437, 788, 1181
ApoI RAATTY 3 cut(s) 915, 1405, 2250
ArsI GACNNNNNNTTYG 4 cut(s) 1196, 1228, 1955, 1987
Asp700I GAANNNNTTC 1 cut(s) 1256
AspLEI GCGC 3 cut(s) 35, 600, 2194
AspS9I GGNCC 6 cut(s) 95, 345, 614, 665, 1627, 2260
AsuC2I CCSGG 2 cut(s) 618, 1277
AsuHPI GGTGA 4 cut(s) 247, 1780, 2089, 2209
AvaI CYCGRG 1 cut(s) 749
AvaII GGWCC 5 cut(s) 95, 345, 665, 1627, 2260
BaeGI GKGCMC 1 cut(s) 1196
BalI TGGCCA 3 cut(s) 1043, 1523, 2247
BanI GGYRCC 4 cut(s) 32, 295, 401, 1191
BarI GAAGNNNNNNTAC 2 cut(s) 890, 922
BauI CACGAG 1 cut(s) 1094
BbrPI CACGTG 1 cut(s) 292
BbsI GAAGAC 1 cut(s) 572
Bbv12I GWGCWC 1 cut(s) 1543
BbvCI CCTCAGC 3 cut(s) 701, 1331, 1649
BbvI GCAGC 3 cut(s) 449, 800, 1193
BccI CCATC 4 cut(s) 1571, 1577, 1843, 2237
BceAI ACGGC 5 cut(s) 166, 341, 710, 851, 1336
BcgI CGANNNNNNTGC 2 cut(s) 1226, 1260
BciT130I CCWGG 3 cut(s) 1045, 1249, 1631
BclI TGATCA 2 cut(s) 1770, 1975
BcnI CCSGG 2 cut(s) 618, 1277
BcoDI GTCTC 5 cut(s) 181, 792, 1317, 2057, 2168
BfaI CTAG 7 cut(s) 473, 545, 684, 822, 873, 2154, 2306
BfoI RGCGCY 3 cut(s) 36, 601, 2195
BfuAI ACCTGC 1 cut(s) 393
BglI GCCNNNNNGGC 1 cut(s) 1436
BglII AGATCT 2 cut(s) 517, 1130
BisI GCNGC 5 cut(s) 438, 498, 789, 1182, 1533
BlpI GCTNAGC 1 cut(s) 1599
BlsI GCNGC 5 cut(s) 439, 499, 790, 1183, 1534
Bme1390I CCNGG 5 cut(s) 618, 1045, 1249, 1277, 1631
Bme18I GGWCC 5 cut(s) 95, 345, 665, 1627, 2260
BmeRI GACNNNNNGTC 1 cut(s) 2155
BmeT110I CYCGRG 1 cut(s) 749
BmgBI CACGTC 2 cut(s) 198, 1675
BmgT120I GGNCC 6 cut(s) 95, 345, 614, 665, 1627, 2260
BmiI GGNNCC 6 cut(s) 34, 297, 403, 615, 666, 1193
BmrFI CCNGG 5 cut(s) 618, 1045, 1249, 1277, 1631
BmsI GCATC 5 cut(s) 343, 822, 844, 2104, 2311
BpiI GAAGAC 1 cut(s) 572
BpmI CTGGAG 1 cut(s) 84
Bpu10I CCTNAGC 3 cut(s) 701, 1331, 1649
Bpu1102I GCTNAGC 1 cut(s) 1599
BpuEI CTTGAG 4 cut(s) 353, 1147, 1313, 2207
BpuMI CCSGG 2 cut(s) 618, 1277
BsaAI YACGTR 2 cut(s) 292, 398
BsaHI GRCGYC 1 cut(s) 33
BsaI GGTCTC 2 cut(s) 181, 1317
BsaJI CCNNGG 3 cut(s) 867, 948, 1754
BsaWI WCCGGW 2 cut(s) 404, 1679
BsaXI ACNNNNNCTCC 8 cut(s) 61, 91, 115, 145, 1578, 1608, 2090, 2120
Bsc4I CCNNNNNNNGG 7 cut(s) 107, 163, 221, 504, 873, 1760, 2269
Bse118I RCCGGY 3 cut(s) 1326, 1535, 2164
Bse3DI GCAATG 2 cut(s) 1242, 1446
BseAI TCCGGA 1 cut(s) 1679
BseBI CCWGG 3 cut(s) 1045, 1249, 1631
BseDI CCNNGG 3 cut(s) 867, 948, 1754
BseGI GGATG 6 cut(s) 676, 928, 1588, 1854, 1866, 2119
BseLI CCNNNNNNNGG 7 cut(s) 107, 163, 221, 504, 873, 1760, 2269
BseMI GCAATG 2 cut(s) 1242, 1446
BseMII CTCAG 7 cut(s) 692, 960, 1322, 1590, 1640, 1805, 2135
BseRI GAGGAG 5 cut(s) 773, 1198, 1310, 1372, 1858
BseSI GKGCMC 1 cut(s) 1196
BseX3I CGGCCG 2 cut(s) 1533, 2255
BseXI GCAGC 3 cut(s) 449, 800, 1193
Bsh1236I CGCG 1 cut(s) 1264
Bsh1285I CGRYCG 2 cut(s) 1536, 2258
BshFI GGCC 9 cut(s) 552, 616, 1043, 1223, 1430, 1523, 1535, 2247, 2257
BshNI GGYRCC 4 cut(s) 32, 295, 401, 1191
BsiEI CGRYCG 2 cut(s) 1536, 2258
BsiHKAI GWGCWC 1 cut(s) 1543
BsiHKCI CYCGRG 1 cut(s) 749
BslFI GGGAC 3 cut(s) 1302, 1708, 2162
BslI CCNNNNNNNGG 7 cut(s) 107, 163, 221, 504, 873, 1760, 2269
BsmAI GTCTC 5 cut(s) 181, 792, 1317, 2057, 2168
BsmBI CGTCTC 1 cut(s) 792
BsmFI GGGAC 3 cut(s) 1302, 1708, 2162
BsmI GAATGC 2 cut(s) 868, 1148
BsnI GGCC 9 cut(s) 552, 616, 1043, 1223, 1430, 1523, 1535, 2247, 2257
Bso31I GGTCTC 2 cut(s) 181, 1317
BsoBI CYCGRG 1 cut(s) 749
Bsp1286I GDGCHC 2 cut(s) 1196, 1543
Bsp13I TCCGGA 1 cut(s) 1679
Bsp1720I GCTNAGC 1 cut(s) 1599
Bsp19I CCATGG 2 cut(s) 948, 1754
BspACI CCGC 9 cut(s) 363, 498, 538, 671, 791, 956, 1262, 1502, 1532
BspANI GGCC 9 cut(s) 552, 616, 1043, 1223, 1430, 1523, 1535, 2247, 2257
BspCNI CTCAG 7 cut(s) 693, 961, 1323, 1591, 1641, 1804, 2136
BspEI TCCGGA 1 cut(s) 1679
BspFNI CGCG 1 cut(s) 1264
BspLI GGNNCC 6 cut(s) 34, 297, 403, 615, 666, 1193
BspMI ACCTGC 1 cut(s) 393
BspPI GGATC 5 cut(s) 174, 599, 681, 979, 1824
BspQI GCTCTTC 1 cut(s) 1743
BspT107I GGYRCC 4 cut(s) 32, 295, 401, 1191
BspTNI GGTCTC 2 cut(s) 181, 1317
BsrBI CCGCTC 1 cut(s) 365
BsrDI GCAATG 2 cut(s) 1242, 1446
BsrFI RCCGGY 3 cut(s) 1326, 1535, 2164
BssAI RCCGGY 3 cut(s) 1326, 1535, 2164
BssECI CCNNGG 3 cut(s) 867, 948, 1754
BssNI GRCGYC 1 cut(s) 33
BssSI CACGAG 1 cut(s) 1094
BssT1I CCWWGG 2 cut(s) 948, 1754
Bst2BI CACGAG 1 cut(s) 1094
Bst2UI CCWGG 3 cut(s) 1045, 1249, 1631
Bst4CI ACNGT 5 cut(s) 115, 239, 772, 1478, 2001
Bst6I CTCTTC 4 cut(s) 90, 573, 1607, 1743
BstACI GRCGYC 1 cut(s) 33
BstAPI GCANNNNNTGC 2 cut(s) 270, 1307
BstBAI YACGTR 2 cut(s) 292, 398
BstC8I GCNNGC 1 cut(s) 1646
BstDEI CTNAG 8 cut(s) 701, 969, 1331, 1599, 1649, 1791, 1820, 2144
BstDSI CCRYGG 3 cut(s) 867, 948, 1754
BstF5I GGATG 6 cut(s) 676, 928, 1588, 1854, 1866, 2119
BstFNI CGCG 1 cut(s) 1264
BstH2I RGCGCY 3 cut(s) 36, 601, 2195
BstHHI GCGC 3 cut(s) 35, 600, 2194
BstMAI GTCTC 5 cut(s) 181, 792, 1317, 2057, 2168
BstMCI CGRYCG 2 cut(s) 1536, 2258
BstNI CCWGG 3 cut(s) 1045, 1249, 1631
BstSCI CCNGG 5 cut(s) 616, 1043, 1247, 1275, 1629
BstSLI GKGCMC 1 cut(s) 1196
BstUI CGCG 1 cut(s) 1264
BstV1I GCAGC 3 cut(s) 449, 800, 1193
BstV2I GAAGAC 1 cut(s) 572
BstX2I RGATCY 4 cut(s) 166, 517, 1130, 1816
BstXI CCANNNNNNTGG 2 cut(s) 342, 1051
BstYI RGATCY 4 cut(s) 166, 517, 1130, 1816
BstZI CGGCCG 2 cut(s) 1533, 2255
BsuRI GGCC 9 cut(s) 552, 616, 1043, 1223, 1430, 1523, 1535, 2247, 2257
BtgI CCRYGG 3 cut(s) 867, 948, 1754
BtgZI GCGATG 1 cut(s) 706
BtrI CACGTC 2 cut(s) 198, 1675
BtsCI GGATG 6 cut(s) 676, 928, 1588, 1854, 1866, 2119
BtsI GCAGTG 1 cut(s) 2124
BtsIMutI CAGTG 3 cut(s) 1613, 1866, 2124
BveI ACCTGC 1 cut(s) 393
Cac8I GCNNGC 1 cut(s) 1646
CfoI GCGC 3 cut(s) 35, 600, 2194
Cfr10I RCCGGY 3 cut(s) 1326, 1535, 2164
Cfr13I GGNCC 6 cut(s) 95, 345, 614, 665, 1627, 2260
CseI GACGC 1 cut(s) 477
CsiI ACCWGGT 1 cut(s) 1629
Csp6I GTAC 4 cut(s) 116, 395, 1273, 2107
CviQI GTAC 4 cut(s) 116, 395, 1273, 2107
DdeI CTNAG 8 cut(s) 701, 969, 1331, 1599, 1649, 1791, 1820, 2144
DinI GGCGCC 1 cut(s) 34
DraIII CACNNNGTG 1 cut(s) 295
DriI GACNNNNNGTC 1 cut(s) 2155
EaeI YGGCCR 5 cut(s) 1041, 1521, 1533, 2245, 2255
EagI CGGCCG 2 cut(s) 1533, 2255
Eam1104I CTCTTC 4 cut(s) 90, 573, 1607, 1743
Eam1105I GACNNNNNGTC 1 cut(s) 2155
EarI CTCTTC 4 cut(s) 90, 573, 1607, 1743
EciI GGCGGA 3 cut(s) 686, 971, 1517
EclXI CGGCCG 2 cut(s) 1533, 2255
Eco130I CCWWGG 2 cut(s) 948, 1754
Eco31I GGTCTC 2 cut(s) 181, 1317
Eco47I GGWCC 5 cut(s) 95, 345, 665, 1627, 2260
Eco47III AGCGCT 1 cut(s) 599
Eco52I CGGCCG 2 cut(s) 1533, 2255
Eco57I CTGAAG 2 cut(s) 1589, 1968
Eco72I CACGTG 1 cut(s) 292
Eco88I CYCGRG 1 cut(s) 749
EcoO109I RGGNCCY 1 cut(s) 1627
EcoRI GAATTC 1 cut(s) 1405
EcoRII CCWGG 3 cut(s) 1043, 1247, 1629
EcoT14I CCWWGG 2 cut(s) 948, 1754
EcoT22I ATGCAT 1 cut(s) 837
EgeI GGCGCC 1 cut(s) 34
EheI GGCGCC 1 cut(s) 34
ErhI CCWWGG 2 cut(s) 948, 1754
Esp3I CGTCTC 1 cut(s) 792
FalI AAGNNNNNCTT 2 cut(s) 2216, 2248
FaqI GGGAC 3 cut(s) 1302, 1708, 2162
FauI CCCGC 1 cut(s) 1269
FbaI TGATCA 2 cut(s) 1770, 1975
FblI GTMKAC 2 cut(s) 627, 2159
Fnu4HI GCNGC 5 cut(s) 438, 498, 789, 1182, 1533
FokI GGATG 6 cut(s) 663, 935, 1595, 1861, 1873, 2126
Fsp4HI GCNGC 5 cut(s) 438, 498, 789, 1182, 1533
FspBI CTAG 7 cut(s) 473, 545, 684, 822, 873, 2154, 2306
GlaI GCGC 3 cut(s) 34, 599, 2193
GluI GCNGC 5 cut(s) 438, 498, 789, 1182, 1533
GsuI CTGGAG 1 cut(s) 84
HaeII RGCGCY 3 cut(s) 36, 601, 2195
HaeIII GGCC 9 cut(s) 552, 616, 1043, 1223, 1430, 1523, 1535, 2247, 2257
HgaI GACGC 1 cut(s) 477
HhaI GCGC 3 cut(s) 35, 600, 2194
Hin1I GRCGYC 1 cut(s) 33
Hin6I GCGC 3 cut(s) 33, 598, 2192
HinP1I GCGC 3 cut(s) 33, 598, 2192
HincII GTYRAC 2 cut(s) 58, 201
HindII GTYRAC 2 cut(s) 58, 201
HindIII AAGCTT 1 cut(s) 647
HphI GGTGA 4 cut(s) 247, 1780, 2089, 2209
Hpy166II GTNNAC 5 cut(s) 58, 201, 395, 628, 2160
Hpy188III TCNNGA 6 cut(s) 101, 545, 562, 751, 1094, 1680
Hpy8I GTNNAC 5 cut(s) 58, 201, 395, 628, 2160
Hpy99I CGWCG 1 cut(s) 493
HpyCH4III ACNGT 5 cut(s) 115, 239, 772, 1478, 2001
HpyCH4IV ACGT 4 cut(s) 197, 291, 397, 1674
HpyCH4V TGCA 4 cut(s) 381, 835, 1798, 2117
HpyF3I CTNAG 8 cut(s) 701, 969, 1331, 1599, 1649, 1791, 1820, 2144
HpySE526I ACGT 4 cut(s) 197, 291, 397, 1674
Hsp92I GRCGYC 1 cut(s) 33
HspAI GCGC 3 cut(s) 33, 598, 2192
KasI GGCGCC 1 cut(s) 32
Kpn2I TCCGGA 1 cut(s) 1679
Ksp22I TGATCA 2 cut(s) 1770, 1975
LguI GCTCTTC 1 cut(s) 1743
LmnI GCTCC 5 cut(s) 136, 336, 1636, 1829, 1930
Lsp1109I GCAGC 3 cut(s) 449, 800, 1193
LweI GCATC 5 cut(s) 343, 822, 844, 2104, 2311
MabI ACCWGGT 1 cut(s) 1629
MaeI CTAG 7 cut(s) 473, 545, 684, 822, 873, 2154, 2306
MaeII ACGT 4 cut(s) 197, 291, 397, 1674
MaeIII GTNAC 5 cut(s) 358, 413, 639, 1670, 2167
MbiI CCGCTC 1 cut(s) 365
MboII GAAGA 8 cut(s) 77, 80, 277, 577, 590, 1162, 1624, 1760
MflI RGATCY 4 cut(s) 166, 517, 1130, 1816
MhlI GDGCHC 2 cut(s) 1196, 1543
MlsI TGGCCA 3 cut(s) 1043, 1523, 2247
MluNI TGGCCA 3 cut(s) 1043, 1523, 2247
Mly113I GGCGCC 1 cut(s) 33
MlyI GAGTC 5 cut(s) 881, 1067, 1199, 1690, 2144
MmeI TCCRAC 2 cut(s) 372, 1716
Mox20I TGGCCA 3 cut(s) 1043, 1523, 2247
Mph1103I ATGCAT 1 cut(s) 837
MroI TCCGGA 1 cut(s) 1679
MroXI GAANNNNTTC 1 cut(s) 1256
MscI TGGCCA 3 cut(s) 1043, 1523, 2247
MseI TTAA 4 cut(s) 635, 659, 693, 2320
MslI CAYNNNNRTG 1 cut(s) 2031
Msp20I TGGCCA 3 cut(s) 1043, 1523, 2247
MspA1I CMGCKG 1 cut(s) 791
MspR9I CCNGG 5 cut(s) 618, 1045, 1249, 1277, 1631
Mva1269I GAATGC 2 cut(s) 868, 1148
MvaI CCWGG 3 cut(s) 1045, 1249, 1631
MvnI CGCG 1 cut(s) 1264
NarI GGCGCC 1 cut(s) 33
NciI CCSGG 2 cut(s) 618, 1277
NcoI CCATGG 2 cut(s) 948, 1754
NlaIV GGNNCC 6 cut(s) 34, 297, 403, 615, 666, 1193
NmeAIII GCCGAG 1 cut(s) 1144
NmuCI GTSAC 3 cut(s) 413, 639, 1670
NsiI ATGCAT 1 cut(s) 837
PaeR7I CTCGAG 1 cut(s) 749
PciSI GCTCTTC 1 cut(s) 1743
PctI GAATGC 2 cut(s) 868, 1148
PdmI GAANNNNTTC 1 cut(s) 1256
PfeI GAWTC 6 cut(s) 529, 1029, 1804, 1894, 2140, 2215
PkrI GCNGC 5 cut(s) 439, 499, 790, 1183, 1534
PleI GAGTC 5 cut(s) 881, 1067, 1199, 1690, 2144
PluTI GGCGCC 1 cut(s) 36
PmaCI CACGTG 1 cut(s) 292
PmlI CACGTG 1 cut(s) 292
PpsI GAGTC 5 cut(s) 881, 1067, 1199, 1690, 2144
Ppu21I YACGTR 2 cut(s) 292, 398
PpuMI RGGWCCY 1 cut(s) 1627
Psp5II RGGWCCY 1 cut(s) 1627
Psp6I CCWGG 3 cut(s) 1043, 1247, 1629
PspCI CACGTG 1 cut(s) 292
PspGI CCWGG 3 cut(s) 1043, 1247, 1629
PspN4I GGNNCC 6 cut(s) 34, 297, 403, 615, 666, 1193
PspPI GGNCC 6 cut(s) 95, 345, 614, 665, 1627, 2260
PspPPI RGGWCCY 1 cut(s) 1627
PsuI RGATCY 4 cut(s) 166, 517, 1130, 1816
RsaI GTAC 4 cut(s) 117, 396, 1274, 2108
RsaNI GTAC 4 cut(s) 116, 395, 1273, 2107
RseI CAYNNNNRTG 1 cut(s) 2031
SapI GCTCTTC 1 cut(s) 1743
SaqAI TTAA 4 cut(s) 635, 659, 693, 2320
SatI GCNGC 5 cut(s) 438, 498, 789, 1182, 1533
Sau96I GGNCC 6 cut(s) 95, 345, 614, 665, 1627, 2260
SchI GAGTC 5 cut(s) 881, 1067, 1199, 1690, 2144
ScrFI CCNGG 5 cut(s) 618, 1045, 1249, 1277, 1631
SduI GDGCHC 2 cut(s) 1196, 1543
SexAI ACCWGGT 1 cut(s) 1629
SfaNI GCATC 5 cut(s) 343, 822, 844, 2104, 2311
SfoI GGCGCC 1 cut(s) 34
Sfr274I CTCGAG 1 cut(s) 749
SinI GGWCC 5 cut(s) 95, 345, 665, 1627, 2260
SlaI CTCGAG 1 cut(s) 749
SmiMI CAYNNNNRTG 1 cut(s) 2031
SmlI CTYRAG 5 cut(s) 368, 749, 1126, 1292, 2222
SmoI CTYRAG 5 cut(s) 368, 749, 1126, 1292, 2222
SsiI CCGC 9 cut(s) 363, 498, 538, 671, 791, 956, 1262, 1502, 1532
SspDI GGCGCC 1 cut(s) 32
SspI AATATT 2 cut(s) 317, 1496
SspMI CTAG 7 cut(s) 473, 545, 684, 822, 873, 2154, 2306
StyD4I CCNGG 5 cut(s) 616, 1043, 1247, 1275, 1629
StyI CCWWGG 2 cut(s) 948, 1754
TaaI ACNGT 5 cut(s) 115, 239, 772, 1478, 2001
TaiI ACGT 4 cut(s) 200, 294, 400, 1677
TaqI TCGA 3 cut(s) 750, 1203, 1215
TaqII GACCGA 2 cut(s) 83, 245
TauI GCSGC 2 cut(s) 500, 1535
TfiI GAWTC 6 cut(s) 529, 1029, 1804, 1894, 2140, 2215
Tru1I TTAA 4 cut(s) 635, 659, 693, 2320
Tru9I TTAA 4 cut(s) 635, 659, 693, 2320
TscAI CASTG 3 cut(s) 1613, 1873, 2124
TseFI GTSAC 3 cut(s) 413, 639, 1670
TseI GCWGC 3 cut(s) 437, 788, 1181
Tsp45I GTSAC 3 cut(s) 413, 639, 1670
TspDTI ATGAA 7 cut(s) 17, 151, 831, 1038, 1453, 1457, 1877
TspGWI ACGGA 2 cut(s) 977, 2186
TspRI CASTG 3 cut(s) 1613, 1873, 2124
VpaK11BI GGWCC 5 cut(s) 95, 345, 665, 1627, 2260
XapI RAATTY 3 cut(s) 915, 1405, 2250
XbaI TCTAGA 1 cut(s) 544
XcmI CCANNNNNNNNNTGG 1 cut(s) 865
XhoI CTCGAG 1 cut(s) 749
XmiI GTMKAC 2 cut(s) 627, 2159
XmnI GAANNNNTTC 1 cut(s) 1256
XspI CTAG 7 cut(s) 473, 545, 684, 822, 873, 2154, 2306
Zsp2I ATGCAT 1 cut(s) 837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.