Prupe.1G218700_v2.0.a1

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000255
Physical Location & Seq
Reverse (-)
23366892 .. 23368817
1926 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G218700.1

Sequence Viewer

Length: 1926 bp
ATGGCTCCTCCCAGTCTTCTCGGTCCTCCAGAGCTCACCAATCCCACCTACACCTCACCGCCCCAATCTCAACCTGTACCCGACCCCGACCGCGACCCCAAACCCGAACCCGCCACCGGCGAACCCTTCGTTGACCTCATGGTCTCAAATTTCAACGACATAGCGATGGACAACAAACTCCCGATGGGCTTGACCGAGAATGATTCCGCCACCTATCTCTCCACCGGCAACCCCTGCCTCGATTTCTTCTTCCACGTGGTGCCGGACACCCCCGCCGACTACGTCAACAACCAGCTCCCGCTGGCCTGGACCCACAGCGCGCCGACCACGCTCAAGCTCATCTGCAACCTCCGCGGCGTACGCGGCACCGGAAAGTCTGACAAGGAACACTTCCACACGGCGGCGGTTTGGCTCCACAACCACCACCCCAAAACCCTCGCCTGCAATCTCAGGTCCTTTGCGGACTTCGGTTATATCAAGGACCTACCCGAAATTCTCTACCGCCTTCTAGAAGGCGAGGACGTGCGGAGGAAACAGAAGGATGCGTTGAAGGAAAGAAAATCCGCCGGCGGCAGGAAGAAACATAGGTTGGGCTGTGACTTGAGCCCCGAAGCCGTTCCGTTCAAGAAAATCAAGACCGGGGGAGAGAAAAAAGCCGCCAAAAAGGACATGTCGAAGGAGGAAAATGAAGCGAAGGAGAAGGAGTGGGTAAAATGGGAAAAGGAAAAGGCAAGCGTGTTGAGGAAGGCGAAGAAGATCGCCATGGCCAAGAAGGCGGTTGCCAAGTATGGATCCGACCCGGATTTCCGATTCTTGCACGAGAGGGTTTCGGATCTTTTCGCCGAGTTGTTGAAGGCCGATATGGAGAATTTGAAGTCGAATCAGAGCAACAAGATCAGCCTCGCGGCCAAGTGGTGCCCTTCGATTGACTCTTCGTTCGATCGAGCCACTCTAATCTGCGAGAGCATTGCGAGAAAGGTGTTCCCGCGCGAATCGTACAAGGAATACGAAGGCATGGAGGAAACCCATTACGCTTACAGAGTGAGAGATCGGCTGAGGAAAGAGGTTCTGGTGCCTCTGAGGAAGGTGCTGGAGTTGCCGGAGGTTTATATGGGAGCGAAGCAGTGGGGTTCGCTTCCGTACAACAGAGTGGCGTCAGTGGCCATGAAGAATTACAAAAGGTTCTTCCTAAAGCACGACAAGGAGAGGTTCAAGAAGTACTTGGAGGACGTGAAGGCCGGCAAGGCGAAGATGGCGGCCGGTGCTCTGCTTCCCCATGAGATTATTGGGTCTGTGGAGGAAGGTGACGTCGGGGAAGTGGCTGAGCTTCAATGGAAGAGAATGGTGGAGGACATGTTGAAGTTGGGGAAGATGAAGAACTGCTTGGCCGTGTGTGACGTGTCCGGTAGCATGTGTGGGACCCCCATGGAGGTGGCGATAGCGTTGGGGCTGTTGGTGTCTGAGCTGAGTGAGGACCCGTGGAAGGGGCTGGTGATCACATTCAGTAGGAATCCTCAGCTGCATTTGGTGAAAGGGGATGATCTGAAGACCAAGTGCCAGTTTGTGAGGGATATGGAGTTCGATATGAACACTGATTTTCAGAAGGTGTTTGATTTGCTGCTGAGGGTTGCGGTGAAGGGGAATTTGAAGCCGGAGAATATGATCAAGAGGGTGTTTGTGTTCAGCGACATGGAGTTTGATGAGGCTTCGGCCAACAGCTGGGAGACTGATTATGACGTGATACAGAAGAAGTTTAGGGAGCATGGTTATGGGAATGCGATCCCACAGCTGGTGTTTTGGAATCTGAGGGACTCGAGGTCTACACCGGTGCCCGGGAATCAGCCCGGGGTGGCTCTGTTGAGTGGCTTCTCTAAGAACTTGCTGAAGCTCTTCATGGACAACGAATGGGGAAATCCGACCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

642

Amino Acids

72.71

Weight (kDa)

8.75

Isoelectric Point (pI)

33.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000255)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24780 AT5G13210 AT5G43390 AT5G43400
fragaria_vesca FvH4_4g23010 FvH4_5g12462 FvH4_5g12490 FvH4_5g12491 FvH4_5g20030 FvH4_5g20031 FvH4_5g21510 FvH4_6g30070
malus_domestica MD00G1095100.v1.1 MD05G1186800.v1.1 MD05G1259500.v1.1 MD05G1260200.v1.1 MD06G1159400.v1.1 MD06G1159500.v1.1 MD06G1162500.v1.1 MD06G1164600.v1.1 MD06G1187800.v1.1 MD06G1212600.v1.1 MD06G1212700.v1.1 MD13G1126800.v1.1 MD14G1168300.v1.1 MD14G1206600.v1.1 MD14G1223900.v1.1 MD14G1230800.v1.1 MD15G1240300.v1.1 MD15G1416800.v1.1 MD16G1127300.v1.1
prunus_persica Prupe.1G218700_v2.0.a1 Prupe.4G045100_v2.0.a1 Prupe.5G159300_v2.0.a1 Prupe.5G172200_v2.0.a1 Prupe.5G172300_v2.0.a1 Prupe.5G173200_v2.0.a1 Prupe.5G173300_v2.0.a1 Prupe.5G173400_v2.0.a1 Prupe.5G219000_v2.0.a1 Prupe.5G219100_v2.0.a1 Prupe.5G219200_v2.0.a1
pyrus_communis pycom05g24390 pycom06g19020 pycom10g15060 pycom10g24800 pycom13g11010 pycom16g10800 pycom16g10810 pycom17g22350 pycom17g22360
rosa_chinensis RchiOBHm_Chr2g0136111 RchiOBHm_Chr4g0429291 RchiOBHm_Chr7g0184981 RchiOBHm_Chr7g0184991 RchiOBHm_Chr7g0185001 RchiOBHm_Chr7g0204841
rosa_laevigata RLG00000003427 RLG00000004941 RLG00000004943 RLG00000004944 RLG00000004945 RLG00000004946 RLG00000007084 RLG00000013701 RLG00000019505 RLG00000019506
rosa_multiflora Rmu_co8294559.1_g000001 Rmu_co8375195.1_g000001 Rmu_co8450783.1_g000001 Rmu_co8519567.1_g000001 Rmu_sc0000054.1_g000008 Rmu_sc0001689.1_g000017 Rmu_sc0001801.1_g000027 Rmu_sc0002070.1_g000010 Rmu_sc0002180.1_g000022 Rmu_sc0002986.1_g000018 Rmu_sc0003221.1_g000027 Rmu_sc0005629.1_g000018 Rmu_sc0009898.1_g000002 Rmu_sc0018428.1_g000003 Rmu_sc0018428.1_g000005 Rmu_sc0022816.1_g000001 Rmu_sc0041781.1_g000001 Rmu_ssc0000289.1_g000007 Rmu_ssc0000289.1_g000008 Rmu_ssc0000289.1_g000010
rosa_roxburghii Rroxscaffold_2G00108460 Rroxscaffold_3G00252720 Rroxscaffold_3G00269290 Rroxscaffold_5G00349680 Rroxscaffold_5G00370900 Rroxscaffold_6G00415020
rosa_rugosa Rorug01G0043400 Rorug02G0328200 Rorug04G0234800 Rorug06G0465600 Rorug06G0465800 Rorug06G0465900 Rorug06G0466000 Rorug06G0466100 Rorug07G0086200 Rorug07G0086200
rosa_samantha Rh2BG386700 Rh2CG365900 Rh2DG402800 Rh4AG290400 Rh4BG296100 Rh4CG311900 Rh4DG293300 Rh7AG077000 Rh7AG077100 Rh7AG077200 Rh7AG216600 Rh7BG070200 Rh7BG070300 Rh7BG070400 Rh7BG213700 Rh7CG071400 Rh7CG071600 Rh7CG071700 Rh7CG071900 Rh7CG230100 Rh7DG070800 Rh7DG070900 Rh7DG224200 Rh7DG440100
rosa_wichuraiana Rw2G030970 Rw4G025150 Rw7G005840 Rw7G005850 Rw7G005860 Rw7G018770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 140
AatII GACGTC 1 cut(s) 1311
AccB1I GGYRCC 5 cut(s) 259, 365, 915, 1072, 1828
AccB7I CCANNNNNTGG 1 cut(s) 1719
AccI GTMKAC 1 cut(s) 1820
AccII CGCG 7 cut(s) 93, 320, 354, 363, 905, 988, 990
AclWI GGATC 4 cut(s) 786, 799, 840, 1774
AcoI YGGCCR 6 cut(s) 765, 906, 1161, 1257, 1386, 1710
AcsI RAATTY 4 cut(s) 148, 492, 868, 1642
AcuI CTGAAG 2 cut(s) 1565, 1904
AcvI CACGTG 1 cut(s) 256
AcyI GRCGYC 2 cut(s) 1154, 1308
AdeI CACNNNGTG 1 cut(s) 259
AfaI GTAC 5 cut(s) 78, 360, 998, 1142, 1220
AfiI CCNNNNNNNGG 9 cut(s) 116, 400, 573, 1429, 1483, 1484, 1719, 1789, 1832
AflIII ACRYGT 3 cut(s) 669, 1353, 1398
AgeI ACCGGT 1 cut(s) 1825
AgsI TTSAA 9 cut(s) 154, 550, 625, 853, 874, 1213, 1331, 1360, 1648
AhdI GACNNNNNGTC 1 cut(s) 1816
AjiI CACGTC 4 cut(s) 523, 1231, 1399, 1738
AjnI CCWGG 1 cut(s) 305
AjuI GAANNNNNNNTTGG 4 cut(s) 572, 604, 1367, 1399
AloI GAACNNNNNNTCC 2 cut(s) 1562, 1594
AluBI AGCT 9 cut(s) 34, 295, 337, 1327, 1465, 1519, 1719, 1789, 1888
AluI AGCT 9 cut(s) 34, 295, 337, 1327, 1465, 1519, 1719, 1789, 1888
Alw21I GWGCWC 2 cut(s) 36, 1267
Alw26I GTCTC 2 cut(s) 148, 1718
AlwI GGATC 4 cut(s) 786, 799, 840, 1774
Ama87I CYCGRG 3 cut(s) 1813, 1832, 1844
AoxI GGCC 9 cut(s) 303, 765, 855, 906, 1161, 1236, 1257, 1386, 1710
ApeKI GCWGC 2 cut(s) 1519, 1618
ApoI RAATTY 4 cut(s) 148, 492, 868, 1642
ArsI GACNNNNNNTTYG 4 cut(s) 920, 952, 1679, 1711
AsiGI ACCGGT 1 cut(s) 1825
Asp700I GAANNNNTTC 2 cut(s) 615, 1889
AspLEI GCGC 3 cut(s) 320, 322, 990
AspS9I GGNCC 6 cut(s) 23, 309, 453, 481, 1419, 1474
AsuC2I CCSGG 6 cut(s) 640, 800, 1833, 1834, 1845, 1846
AsuHPI GGTGA 6 cut(s) 28, 48, 1316, 1504, 1540, 1645
AvaI CYCGRG 3 cut(s) 1813, 1832, 1844
AvaII GGWCC 6 cut(s) 23, 309, 453, 481, 1419, 1474
BaeGI GKGCMC 2 cut(s) 920, 1833
BalI TGGCCA 2 cut(s) 767, 1163
BamHI GGATCC 1 cut(s) 791
BanI GGYRCC 5 cut(s) 259, 365, 915, 1072, 1828
BanII GRGCYC 2 cut(s) 36, 608
BauI CACGAG 1 cut(s) 818
BbrPI CACGTG 1 cut(s) 256
BbsI GAAGAC 2 cut(s) 8, 1553
Bbv12I GWGCWC 2 cut(s) 36, 1267
BbvCI CCTCAGC 3 cut(s) 1055, 1515, 1622
BbvI GCAGC 2 cut(s) 1506, 1605
BccI CCATC 3 cut(s) 160, 178, 1246
BceAI ACGGC 3 cut(s) 414, 599, 1373
BcgI CGANNNNNNTGC 2 cut(s) 950, 984
BciT130I CCWGG 1 cut(s) 307
BclI TGATCA 2 cut(s) 1494, 1662
BcnI CCSGG 6 cut(s) 640, 800, 1833, 1834, 1845, 1846
BcoDI GTCTC 2 cut(s) 148, 1718
BfaI CTAG 1 cut(s) 509
BglI GCCNNNNNGGC 2 cut(s) 773, 1244
BisI GCNGC 9 cut(s) 355, 364, 402, 571, 657, 906, 1257, 1520, 1619
BlpI GCTNAGC 1 cut(s) 1323
BlsI GCNGC 9 cut(s) 356, 365, 403, 572, 658, 907, 1258, 1521, 1620
BmcAI AGTACT 1 cut(s) 1220
Bme1390I CCNGG 7 cut(s) 307, 640, 800, 1833, 1834, 1845, 1846
Bme18I GGWCC 6 cut(s) 23, 309, 453, 481, 1419, 1474
BmeRI GACNNNNNGTC 1 cut(s) 1816
BmeT110I CYCGRG 3 cut(s) 1813, 1832, 1844
BmgBI CACGTC 4 cut(s) 523, 1231, 1399, 1738
BmgT120I GGNCC 6 cut(s) 23, 309, 453, 481, 1419, 1474
BmrFI CCNGG 7 cut(s) 307, 640, 800, 1833, 1834, 1845, 1846
BmrI ACTGGG 1 cut(s) 6
BmsI GCATC 1 cut(s) 532
BmuI ACTGGG 1 cut(s) 6
BpiI GAAGAC 2 cut(s) 8, 1553
BpmI CTGGAG 2 cut(s) 12, 1112
Bpu10I CCTNAGC 3 cut(s) 1055, 1515, 1622
Bpu1102I GCTNAGC 1 cut(s) 1323
BpuEI CTTGAG 2 cut(s) 317, 622
BpuMI CCSGG 6 cut(s) 640, 800, 1833, 1834, 1845, 1846
BsaAI YACGTR 1 cut(s) 256
BsaHI GRCGYC 2 cut(s) 1154, 1308
BsaI GGTCTC 1 cut(s) 148
BsaJI CCNNGG 8 cut(s) 352, 639, 762, 1425, 1478, 1832, 1844, 1845
BsaWI WCCGGW 3 cut(s) 368, 1403, 1825
BsaXI ACNNNNNCTCC 4 cut(s) 689, 719, 1340, 1370
Bsc4I CCNNNNNNNGG 9 cut(s) 116, 400, 573, 1429, 1483, 1484, 1719, 1789, 1832
Bse118I RCCGGY 6 cut(s) 116, 224, 566, 1238, 1259, 1825
Bse1I ACTGG 2 cut(s) 12, 1558
Bse3DI GCAATG 1 cut(s) 966
BseBI CCWGG 1 cut(s) 307
BseDI CCNNGG 8 cut(s) 352, 639, 762, 1425, 1478, 1832, 1844, 1845
BseGI GGATG 2 cut(s) 547, 1543
BseLI CCNNNNNNNGG 9 cut(s) 116, 400, 573, 1429, 1483, 1484, 1719, 1789, 1832
BseMI GCAATG 1 cut(s) 966
BseMII CTCAG 9 cut(s) 463, 1046, 1070, 1314, 1452, 1457, 1529, 1613, 1796
BseNI ACTGG 2 cut(s) 12, 1558
BsePI GCGCGC 1 cut(s) 318
BseSI GKGCMC 2 cut(s) 920, 1833
BseX3I CGGCCG 1 cut(s) 1257
BseXI GCAGC 2 cut(s) 1506, 1605
BseYI CCCAGC 1 cut(s) 1719
Bsh1236I CGCG 7 cut(s) 93, 320, 354, 363, 905, 988, 990
Bsh1285I CGRYCG 3 cut(s) 91, 943, 1260
BshFI GGCC 9 cut(s) 305, 767, 857, 908, 1163, 1238, 1259, 1388, 1712
BshNI GGYRCC 5 cut(s) 259, 365, 915, 1072, 1828
BshTI ACCGGT 1 cut(s) 1825
BsiEI CGRYCG 3 cut(s) 91, 943, 1260
BsiHKAI GWGCWC 2 cut(s) 36, 1267
BsiHKCI CYCGRG 3 cut(s) 1813, 1832, 1844
BsiWI CGTACG 1 cut(s) 358
BslFI GGGAC 2 cut(s) 1432, 1823
BslI CCNNNNNNNGG 9 cut(s) 116, 400, 573, 1429, 1483, 1484, 1719, 1789, 1832
BsmAI GTCTC 2 cut(s) 148, 1718
BsmFI GGGAC 2 cut(s) 1432, 1823
BsmI GAATGC 1 cut(s) 1780
BsnI GGCC 9 cut(s) 305, 767, 857, 908, 1163, 1238, 1259, 1388, 1712
Bso31I GGTCTC 1 cut(s) 148
BsoBI CYCGRG 3 cut(s) 1813, 1832, 1844
Bsp1286I GDGCHC 5 cut(s) 36, 608, 920, 1267, 1833
Bsp1720I GCTNAGC 1 cut(s) 1323
Bsp19I CCATGG 2 cut(s) 762, 1425
BspANI GGCC 9 cut(s) 305, 767, 857, 908, 1163, 1238, 1259, 1388, 1712
BspCNI CTCAG 9 cut(s) 462, 1047, 1071, 1315, 1453, 1458, 1528, 1614, 1797
BspFNI CGCG 7 cut(s) 93, 320, 354, 363, 905, 988, 990
BspPI GGATC 4 cut(s) 786, 799, 840, 1774
BspQI GCTCTTC 1 cut(s) 1895
BspT107I GGYRCC 5 cut(s) 259, 365, 915, 1072, 1828
BspTNI GGTCTC 1 cut(s) 148
BsrDI GCAATG 1 cut(s) 966
BsrFI RCCGGY 6 cut(s) 116, 224, 566, 1238, 1259, 1825
BsrI ACTGG 2 cut(s) 12, 1558
BssAI RCCGGY 6 cut(s) 116, 224, 566, 1238, 1259, 1825
BssECI CCNNGG 8 cut(s) 352, 639, 762, 1425, 1478, 1832, 1844, 1845
BssHII GCGCGC 1 cut(s) 318
BssNI GRCGYC 2 cut(s) 1154, 1308
BssSI CACGAG 1 cut(s) 818
BssT1I CCWWGG 2 cut(s) 762, 1425
Bst2BI CACGAG 1 cut(s) 818
Bst2UI CCWGG 1 cut(s) 307
Bst6I CTCTTC 3 cut(s) 937, 1331, 1895
BstACI GRCGYC 2 cut(s) 1154, 1308
BstAPI GCANNNNNTGC 1 cut(s) 234
BstBAI YACGTR 1 cut(s) 256
BstC8I GCNNGC 6 cut(s) 303, 320, 442, 568, 733, 1240
BstDSI CCRYGG 4 cut(s) 352, 762, 1425, 1478
BstF5I GGATG 2 cut(s) 547, 1543
BstFNI CGCG 7 cut(s) 93, 320, 354, 363, 905, 988, 990
BstHHI GCGC 3 cut(s) 320, 322, 990
BstMAI GTCTC 2 cut(s) 148, 1718
BstMCI CGRYCG 3 cut(s) 91, 943, 1260
BstNI CCWGG 1 cut(s) 307
BstNSI RCATGY 3 cut(s) 673, 1357, 1414
BstSCI CCNGG 7 cut(s) 305, 638, 798, 1831, 1832, 1843, 1844
BstSLI GKGCMC 2 cut(s) 920, 1833
BstUI CGCG 7 cut(s) 93, 320, 354, 363, 905, 988, 990
BstV1I GCAGC 2 cut(s) 1506, 1605
BstV2I GAAGAC 2 cut(s) 8, 1553
BstX2I RGATCY 2 cut(s) 791, 832
BstXI CCANNNNNNTGG 1 cut(s) 1432
BstYI RGATCY 2 cut(s) 791, 832
BstZI CGGCCG 1 cut(s) 1257
BsuRI GGCC 9 cut(s) 305, 767, 857, 908, 1163, 1238, 1259, 1388, 1712
BtgI CCRYGG 4 cut(s) 352, 762, 1425, 1478
BtgZI GCGATG 1 cut(s) 179
BtrI CACGTC 4 cut(s) 523, 1231, 1399, 1738
BtsCI GGATG 2 cut(s) 547, 1543
BtsI GCAGTG 1 cut(s) 1130
BtsIMutI CAGTG 3 cut(s) 1130, 1164, 1590
Cac8I GCNNGC 6 cut(s) 303, 320, 442, 568, 733, 1240
CfoI GCGC 3 cut(s) 320, 322, 990
Cfr10I RCCGGY 6 cut(s) 116, 224, 566, 1238, 1259, 1825
Cfr13I GGNCC 6 cut(s) 23, 309, 453, 481, 1419, 1474
Cfr42I CCGCGG 1 cut(s) 355
Cfr9I CCCGGG 2 cut(s) 1832, 1844
CseI GACGC 1 cut(s) 1143
Csp6I GTAC 5 cut(s) 77, 359, 997, 1141, 1219
CspAI ACCGGT 1 cut(s) 1825
CviQI GTAC 5 cut(s) 77, 359, 997, 1141, 1219
DraIII CACNNNGTG 1 cut(s) 259
DrdI GACNNNNNNGTC 1 cut(s) 140
DriI GACNNNNNGTC 1 cut(s) 1816
DseDI GACNNNNNNGTC 1 cut(s) 140
EaeI YGGCCR 6 cut(s) 765, 906, 1161, 1257, 1386, 1710
EagI CGGCCG 1 cut(s) 1257
Eam1104I CTCTTC 3 cut(s) 937, 1331, 1895
Eam1105I GACNNNNNGTC 1 cut(s) 1816
EarI CTCTTC 3 cut(s) 937, 1331, 1895
EciI GGCGGA 2 cut(s) 196, 553
Ecl136II GAGCTC 1 cut(s) 34
EclXI CGGCCG 1 cut(s) 1257
Eco130I CCWWGG 2 cut(s) 762, 1425
Eco24I GRGCYC 2 cut(s) 36, 608
Eco31I GGTCTC 1 cut(s) 148
Eco47I GGWCC 6 cut(s) 23, 309, 453, 481, 1419, 1474
Eco52I CGGCCG 1 cut(s) 1257
Eco53kI GAGCTC 1 cut(s) 34
Eco57I CTGAAG 2 cut(s) 1565, 1904
Eco72I CACGTG 1 cut(s) 256
Eco88I CYCGRG 3 cut(s) 1813, 1832, 1844
EcoICRI GAGCTC 1 cut(s) 34
EcoO109I RGGNCCY 4 cut(s) 453, 481, 1419, 1474
EcoRII CCWGG 1 cut(s) 305
EcoT14I CCWWGG 2 cut(s) 762, 1425
EcoT38I GRGCYC 2 cut(s) 36, 608
ErhI CCWWGG 2 cut(s) 762, 1425
FalI AAGNNNNNCTT 6 cut(s) 374, 406, 1205, 1237, 1367, 1399
FaqI GGGAC 2 cut(s) 1432, 1823
FauI CCCGC 4 cut(s) 118, 280, 306, 993
FbaI TGATCA 2 cut(s) 1494, 1662
FblI GTMKAC 1 cut(s) 1820
Fnu4HI GCNGC 9 cut(s) 355, 364, 402, 571, 657, 906, 1257, 1520, 1619
FokI GGATG 2 cut(s) 554, 1550
FriOI GRGCYC 2 cut(s) 36, 608
Fsp4HI GCNGC 9 cut(s) 355, 364, 402, 571, 657, 906, 1257, 1520, 1619
FspBI CTAG 1 cut(s) 509
GlaI GCGC 3 cut(s) 319, 321, 989
GluI GCNGC 9 cut(s) 355, 364, 402, 571, 657, 906, 1257, 1520, 1619
GsaI CCCAGC 1 cut(s) 1723
GsuI CTGGAG 2 cut(s) 12, 1112
HaeIII GGCC 9 cut(s) 305, 767, 857, 908, 1163, 1238, 1259, 1388, 1712
HgaI GACGC 1 cut(s) 1143
HhaI GCGC 3 cut(s) 320, 322, 990
Hin1I GRCGYC 2 cut(s) 1154, 1308
Hin6I GCGC 3 cut(s) 318, 320, 988
HinP1I GCGC 3 cut(s) 318, 320, 988
HincII GTYRAC 2 cut(s) 133, 286
HindII GTYRAC 2 cut(s) 133, 286
HinfI GANTC 9 cut(s) 203, 810, 880, 929, 992, 1510, 1801, 1811, 1837
HphI GGTGA 6 cut(s) 28, 48, 1316, 1504, 1540, 1645
Hpy166II GTNNAC 3 cut(s) 133, 286, 1821
Hpy188III TCNNGA 7 cut(s) 29, 181, 509, 625, 634, 1213, 1666
Hpy8I GTNNAC 3 cut(s) 133, 286, 1821
Hpy99I CGWCG 1 cut(s) 1313
HpyCH4IV ACGT 7 cut(s) 255, 282, 522, 1230, 1308, 1398, 1737
HpyCH4V TGCA 4 cut(s) 345, 444, 817, 1522
HpySE526I ACGT 7 cut(s) 255, 282, 522, 1230, 1308, 1398, 1737
Hsp92I GRCGYC 2 cut(s) 1154, 1308
HspAI GCGC 3 cut(s) 318, 320, 988
KflI GGGWCCC 1 cut(s) 1419
KroI GCCGGC 2 cut(s) 566, 1238
KroNI GCCGGC 2 cut(s) 568, 1240
Ksp22I TGATCA 2 cut(s) 1494, 1662
KspI CCGCGG 1 cut(s) 355
LguI GCTCTTC 1 cut(s) 1895
LmnI GCTCC 5 cut(s) 10, 300, 417, 1115, 1759
Lsp1109I GCAGC 2 cut(s) 1506, 1605
LweI GCATC 1 cut(s) 532
MaeI CTAG 1 cut(s) 509
MaeII ACGT 7 cut(s) 255, 282, 522, 1230, 1308, 1398, 1737
MaeIII GTNAC 3 cut(s) 596, 1304, 1394
MflI RGATCY 2 cut(s) 791, 832
MhlI GDGCHC 5 cut(s) 36, 608, 920, 1267, 1833
MlsI TGGCCA 2 cut(s) 767, 1163
MluCI AATT 5 cut(s) 148, 492, 868, 1171, 1642
MluNI TGGCCA 2 cut(s) 767, 1163
MlyI GAGTC 2 cut(s) 923, 1805
MmeI TCCRAC 1 cut(s) 819
Mox20I TGGCCA 2 cut(s) 767, 1163
MreI CGCCGGCG 1 cut(s) 566
MroNI GCCGGC 2 cut(s) 566, 1238
MroXI GAANNNNTTC 2 cut(s) 615, 1889
MscI TGGCCA 2 cut(s) 767, 1163
MslI CAYNNNNRTG 3 cut(s) 164, 1430, 1767
Msp20I TGGCCA 2 cut(s) 767, 1163
MspA1I CMGCKG 5 cut(s) 301, 354, 1519, 1719, 1789
MspR9I CCNGG 7 cut(s) 307, 640, 800, 1833, 1834, 1845, 1846
Mva1269I GAATGC 1 cut(s) 1780
MvaI CCWGG 1 cut(s) 307
MvnI CGCG 7 cut(s) 93, 320, 354, 363, 905, 988, 990
NaeI GCCGGC 2 cut(s) 568, 1240
NciI CCSGG 6 cut(s) 640, 800, 1833, 1834, 1845, 1846
NcoI CCATGG 2 cut(s) 762, 1425
NgoMIV GCCGGC 2 cut(s) 566, 1238
NmeAIII GCCGAG 1 cut(s) 868
NmuCI GTSAC 3 cut(s) 596, 1304, 1394
NspI RCATGY 3 cut(s) 673, 1357, 1414
PaeR7I CTCGAG 1 cut(s) 1813
PauI GCGCGC 1 cut(s) 318
PciI ACATGT 2 cut(s) 669, 1353
PciSI GCTCTTC 1 cut(s) 1895
PctI GAATGC 1 cut(s) 1780
PdiI GCCGGC 2 cut(s) 568, 1240
PdmI GAANNNNTTC 2 cut(s) 615, 1889
PfeI GAWTC 7 cut(s) 203, 810, 880, 992, 1510, 1801, 1837
Pfl23II CGTACG 1 cut(s) 358
PflFI GACNNNGTC 1 cut(s) 281
PflMI CCANNNNNTGG 1 cut(s) 1719
PinAI ACCGGT 1 cut(s) 1825
PkrI GCNGC 9 cut(s) 356, 365, 403, 572, 658, 907, 1258, 1521, 1620
Ple19I CGATCG 1 cut(s) 943
PleI GAGTC 2 cut(s) 923, 1805
PmaCI CACGTG 1 cut(s) 256
PmlI CACGTG 1 cut(s) 256
PpsI GAGTC 2 cut(s) 923, 1805
Ppu21I YACGTR 1 cut(s) 256
PpuMI RGGWCCY 4 cut(s) 453, 481, 1419, 1474
PscI ACATGT 2 cut(s) 669, 1353
Psp124BI GAGCTC 1 cut(s) 36
Psp5II RGGWCCY 4 cut(s) 453, 481, 1419, 1474
Psp6I CCWGG 1 cut(s) 305
PspCI CACGTG 1 cut(s) 256
PspFI CCCAGC 1 cut(s) 1719
PspGI CCWGG 1 cut(s) 305
PspLI CGTACG 1 cut(s) 358
PspPI GGNCC 6 cut(s) 23, 309, 453, 481, 1419, 1474
PspPPI RGGWCCY 4 cut(s) 453, 481, 1419, 1474
PspXI VCTCGAGB 1 cut(s) 1813
PsuI RGATCY 2 cut(s) 791, 832
PsyI GACNNNGTC 1 cut(s) 281
PteI GCGCGC 1 cut(s) 318
PvuI CGATCG 1 cut(s) 943
PvuII CAGCTG 3 cut(s) 1519, 1719, 1789
RsaI GTAC 5 cut(s) 78, 360, 998, 1142, 1220
RsaNI GTAC 5 cut(s) 77, 359, 997, 1141, 1219
RseI CAYNNNNRTG 3 cut(s) 164, 1430, 1767
SacI GAGCTC 1 cut(s) 36
SacII CCGCGG 1 cut(s) 355
SapI GCTCTTC 1 cut(s) 1895
SatI GCNGC 9 cut(s) 355, 364, 402, 571, 657, 906, 1257, 1520, 1619
Sau96I GGNCC 6 cut(s) 23, 309, 453, 481, 1419, 1474
ScaI AGTACT 1 cut(s) 1220
SchI GAGTC 2 cut(s) 923, 1805
ScrFI CCNGG 7 cut(s) 307, 640, 800, 1833, 1834, 1845, 1846
SduI GDGCHC 5 cut(s) 36, 608, 920, 1267, 1833
SfaNI GCATC 1 cut(s) 532
Sfr274I CTCGAG 1 cut(s) 1813
Sfr303I CCGCGG 1 cut(s) 355
SgrAI CRCCGGYG 3 cut(s) 116, 566, 1825
SgrBI CCGCGG 1 cut(s) 355
SinI GGWCC 6 cut(s) 23, 309, 453, 481, 1419, 1474
SlaI CTCGAG 1 cut(s) 1813
SmaI CCCGGG 2 cut(s) 1834, 1846
SmiMI CAYNNNNRTG 3 cut(s) 164, 1430, 1767
SmlI CTYRAG 3 cut(s) 332, 601, 1813
SmoI CTYRAG 3 cut(s) 332, 601, 1813
Sse9I AATT 5 cut(s) 148, 492, 868, 1171, 1642
SspMI CTAG 1 cut(s) 509
SstI GAGCTC 1 cut(s) 36
StyD4I CCNGG 7 cut(s) 305, 638, 798, 1831, 1832, 1843, 1844
StyI CCWWGG 2 cut(s) 762, 1425
TaiI ACGT 7 cut(s) 258, 285, 525, 1233, 1311, 1401, 1740
TaqI TCGA 8 cut(s) 240, 674, 878, 923, 939, 943, 1581, 1814
TaqII GACCGA 2 cut(s) 11, 209
TasI AATT 5 cut(s) 148, 492, 868, 1171, 1642
TatI WGTACW 1 cut(s) 1218
TauI GCSGC 7 cut(s) 357, 366, 404, 573, 659, 908, 1259
TfiI GAWTC 7 cut(s) 203, 810, 880, 992, 1510, 1801, 1837
TscAI CASTG 3 cut(s) 1130, 1164, 1597
TseFI GTSAC 3 cut(s) 596, 1304, 1394
TseI GCWGC 2 cut(s) 1519, 1618
Tsp45I GTSAC 3 cut(s) 596, 1304, 1394
TspDTI ATGAA 5 cut(s) 702, 1181, 1388, 1601, 1882
TspGWI ACGGA 2 cut(s) 609, 1128
TspMI CCCGGG 2 cut(s) 1832, 1844
TspRI CASTG 3 cut(s) 1130, 1164, 1597
Tth111I GACNNNGTC 1 cut(s) 281
Van91I CCANNNNNTGG 1 cut(s) 1719
VpaK11BI GGWCC 6 cut(s) 23, 309, 453, 481, 1419, 1474
XapI RAATTY 4 cut(s) 148, 492, 868, 1642
XbaI TCTAGA 1 cut(s) 508
XceI RCATGY 3 cut(s) 673, 1357, 1414
XcmI CCANNNNNNNNNTGG 1 cut(s) 1283
XhoI CTCGAG 1 cut(s) 1813
XmaI CCCGGG 2 cut(s) 1832, 1844
XmiI GTMKAC 1 cut(s) 1820
XmnI GAANNNNTTC 2 cut(s) 615, 1889
XspI CTAG 1 cut(s) 509
ZraI GACGTC 1 cut(s) 1309
ZrmI AGTACT 1 cut(s) 1220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.