MD16G1127300.v1.1

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000255
Physical Location & Seq
Forward (+)
9254653 .. 9256581
1929 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1127300.v1.1.491

Sequence Viewer

Length: 1929 bp
ATGGCTCCTCCAAGCCTACTCGGTCCTCCAGAGCTCAGCAACCCCACCAAGACCACATCCGAGCCCGAACCCCCCACCGGCGAACCCTTGGTCGACTTGTTGGTCTCGGATTTCAACAACAAATCGATGGAAGATGAGCCCGAGATGGGCCTGACGGAGAACGAATCCGCCACCTACGTCTCCACCGGCAACCCCTGCCTCGATCTCTTCTTCCACGTCGTTCCGGACACCCCCGCCGATTACCTGAACGAGCAACTCGCACAGGCCTGGGACCAGAACGCTCTGACCACGCTCAAGCTTATCTGCAACCTCCGAGGCGTGCGCGGCACCGGGAAGTCCGACAAGGAGAACTTCCACACGGCGGCGTTTTGGCTCCACAAGCACCACCCCAAAACCCTCGCCTGCAATCTCAAAGCGTTTGTGGAATTCGGGTATTTCAAGGACCTCCCGGAGATTCTATACCGGCTTCTGGAAGGCGAGGACGTGCGGAGGAACCAGAGGGAGGTGTGGAAGACCCAAAAATCGTGCGTTGGAAATAAGATTCGGACTTGGGGACCGTTCAAGAAAATCGGAGGAAAATGGGAGAAGAACCCCGTCAAAAAGGTGCTGACGGAAGAGGAAAAGAAGGCGAAGCAAGAGAAGTTGAGGGAGTTGGCAAAATTGGAGAAGGAAAATGCGAGCAGGCTGAGGAAGGAGAAGAAGGTCGCCATGGCCAAGAAGGCGGTGGCCCGGTACGAGCACGATCCGGATTTCAGATTCCTGCACGAGCGCATTTCGGATATTTTCGCCGAGTTTCTGAAGGCCGATATGGAAAGTTTGAAGTCGAATCAGCGCAACAAGATCACCCTGGCGGCGAAGTGGTGCCCTTCGATCGACTCCTCGTTCGATCAGGTCACTCTGATCTGCGAGAGCATCGCGAGGAAGGTTTTCCCGCGCGAATCGTACAAGGAATACGACGGCGTTGAAGAAGCCCATTACGCGTACAAAGTGAGGGACCGGCTGAGGAAGGAGGTGCTGGTGCCGCTGAGGAAGATTCTGGAGCTGCCGGAGGTGTACATGGGGGCCAACGAGTGGGGTTCAATTCTGTACAACAGGGTGGCGTCGGTGGCGATGAAGAATTACAAGAAGTCCTTCTTGAAGCATGACGAAGAGCGGTTCAAGAAGTACTTGGAGGACGTGAAGACGGGCAAGGCGAAGATTGCTGCCGGAGCCCTGCTGCCCCACGAGATTATTGGGTCTCTAGGGGACGGTGATGTTGGGCAGGTGGCGGATCTTCAGTGGAAGAGAATGGTGGAGGACATGTTGAAGTTGGGTAAGATGAAGAACTGCTTGGCCGTGTGTGACGTGTCCGGGAGCATGGAAGGGACTCCAATGGAGGTGTCGGTGGCGTTGGGGCTGCTGGTATCTGAGCTGAGCGAGGACCCCTGGAAAGGGCTGGTGATCACATTCAGTGAGAATCCTCAGCTGCATTTGGTGGAAGGGGATGATCTGATGACCAAGTGCCAGTTTATGAGGGAGATGGATTGGGGGATGAACACCGATTTCCAGCAGGTGTTTGATTTGCTGCTGAGCGTTGCGGTGAAGGGGAATTTGAAACCGGAGAATATGATCAAGAGGATTTTCGTGTTTAGTGACATGGAGTTTGATCAGGCTTCGCCCAACAGCTGGGAGACGGATTATGACGTGATACAAAAGAAGTTTAGGGAGAGCGGGTATGGTGACGCGATTCCGCAGCTGGTGTTTTGGAATCTGAGGGACTCGAGGTCTGTACCGGTGCCGGGGAACCAGCCTGGGGTGGCTCTGCTGAGTGGCTTCTCAAAGAATCTGCTGAAGTTGTTCCTGGATAATGACGGGGAAGTTCGACCGGATGTGTTCATGGAATTAGCGATATCGGGAGAAGAGTATGAGAAGCTAGTTGTGGTCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

643

Amino Acids

73.13

Weight (kDa)

5.79

Isoelectric Point (pI)

31.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2828 PF11443 52 - 440 5.2e-138 Domain of unknown function (DUF2828)
DUF7788 PF25043 442 - 620 2.5e-79 Domain of unknown function (DUF7788)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000255)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24780 AT5G13210 AT5G43390 AT5G43400
fragaria_vesca FvH4_4g23010 FvH4_5g12462 FvH4_5g12490 FvH4_5g12491 FvH4_5g20030 FvH4_5g20031 FvH4_5g21510 FvH4_6g30070
malus_domestica MD00G1095100.v1.1 MD05G1186800.v1.1 MD05G1259500.v1.1 MD05G1260200.v1.1 MD06G1159400.v1.1 MD06G1159500.v1.1 MD06G1162500.v1.1 MD06G1164600.v1.1 MD06G1187800.v1.1 MD06G1212600.v1.1 MD06G1212700.v1.1 MD13G1126800.v1.1 MD14G1168300.v1.1 MD14G1206600.v1.1 MD14G1223900.v1.1 MD14G1230800.v1.1 MD15G1240300.v1.1 MD15G1416800.v1.1 MD16G1127300.v1.1
prunus_persica Prupe.1G218700_v2.0.a1 Prupe.4G045100_v2.0.a1 Prupe.5G159300_v2.0.a1 Prupe.5G172200_v2.0.a1 Prupe.5G172300_v2.0.a1 Prupe.5G173200_v2.0.a1 Prupe.5G173300_v2.0.a1 Prupe.5G173400_v2.0.a1 Prupe.5G219000_v2.0.a1 Prupe.5G219100_v2.0.a1 Prupe.5G219200_v2.0.a1
pyrus_communis pycom05g24390 pycom06g19020 pycom10g15060 pycom10g24800 pycom13g11010 pycom16g10800 pycom16g10810 pycom17g22350 pycom17g22360
rosa_chinensis RchiOBHm_Chr2g0136111 RchiOBHm_Chr4g0429291 RchiOBHm_Chr7g0184981 RchiOBHm_Chr7g0184991 RchiOBHm_Chr7g0185001 RchiOBHm_Chr7g0204841
rosa_laevigata RLG00000003427 RLG00000004941 RLG00000004943 RLG00000004944 RLG00000004945 RLG00000004946 RLG00000007084 RLG00000013701 RLG00000019505 RLG00000019506
rosa_multiflora Rmu_co8294559.1_g000001 Rmu_co8375195.1_g000001 Rmu_co8450783.1_g000001 Rmu_co8519567.1_g000001 Rmu_sc0000054.1_g000008 Rmu_sc0001689.1_g000017 Rmu_sc0001801.1_g000027 Rmu_sc0002070.1_g000010 Rmu_sc0002180.1_g000022 Rmu_sc0002986.1_g000018 Rmu_sc0003221.1_g000027 Rmu_sc0005629.1_g000018 Rmu_sc0009898.1_g000002 Rmu_sc0018428.1_g000003 Rmu_sc0018428.1_g000005 Rmu_sc0022816.1_g000001 Rmu_sc0041781.1_g000001 Rmu_ssc0000289.1_g000007 Rmu_ssc0000289.1_g000008 Rmu_ssc0000289.1_g000010
rosa_roxburghii Rroxscaffold_2G00108460 Rroxscaffold_3G00252720 Rroxscaffold_3G00269290 Rroxscaffold_5G00349680 Rroxscaffold_5G00370900 Rroxscaffold_6G00415020
rosa_rugosa Rorug01G0043400 Rorug02G0328200 Rorug04G0234800 Rorug06G0465600 Rorug06G0465800 Rorug06G0465900 Rorug06G0466000 Rorug06G0466100 Rorug07G0086200 Rorug07G0086200
rosa_samantha Rh2BG386700 Rh2CG365900 Rh2DG402800 Rh4AG290400 Rh4BG296100 Rh4CG311900 Rh4DG293300 Rh7AG077000 Rh7AG077100 Rh7AG077200 Rh7AG216600 Rh7BG070200 Rh7BG070300 Rh7BG070400 Rh7BG213700 Rh7CG071400 Rh7CG071600 Rh7CG071700 Rh7CG071900 Rh7CG230100 Rh7DG070800 Rh7DG070900 Rh7DG224200 Rh7DG440100
rosa_wichuraiana Rw2G030970 Rw4G025150 Rw7G005840 Rw7G005850 Rw7G005860 Rw7G018770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1252, 1540
AasI GACNNNNNNGTC 1 cut(s) 101
Acc36I ACCTGC 2 cut(s) 1252, 1540
AccB1I GGYRCC 4 cut(s) 326, 861, 1018, 1774
AccB7I CCANNNNNTGG 2 cut(s) 1071, 1665
AccBSI CCGCTC 2 cut(s) 1153, 1710
AccI GTMKAC 1 cut(s) 93
AccII CGCG 6 cut(s) 324, 917, 934, 936, 980, 1724
AccIII TCCGGA 2 cut(s) 223, 745
AclWI GGATC 2 cut(s) 737, 1278
AcoI YGGCCR 2 cut(s) 711, 1332
AcsI RAATTY 2 cut(s) 425, 1588
AcuI CTGAAG 3 cut(s) 818, 1259, 1850
AcyI GRCGYC 1 cut(s) 1100
AfaI GTAC 7 cut(s) 734, 944, 983, 1055, 1088, 1166, 1770
AflIII ACRYGT 3 cut(s) 978, 1299, 1344
AgeI ACCGGT 1 cut(s) 1771
AhdI GACNNNNNGTC 1 cut(s) 1762
AjiI CACGTC 5 cut(s) 217, 484, 1177, 1345, 1684
AjnI CCWGG 5 cut(s) 266, 846, 1424, 1789, 1839
AjuI GAANNNNNNNTTGG 2 cut(s) 1313, 1345
AluBI AGCT 8 cut(s) 34, 298, 1042, 1411, 1465, 1665, 1735, 1912
AluI AGCT 8 cut(s) 34, 298, 1042, 1411, 1465, 1665, 1735, 1912
Alw21I GWGCWC 2 cut(s) 36, 741
Alw26I GTCTC 4 cut(s) 109, 184, 1242, 1664
AlwI GGATC 2 cut(s) 737, 1278
Ama87I CYCGRG 2 cut(s) 140, 1759
Aor13HI TCCGGA 2 cut(s) 223, 745
AoxI GGCC 7 cut(s) 148, 264, 711, 726, 801, 1062, 1332
ApeKI GCWGC 7 cut(s) 1042, 1202, 1216, 1396, 1465, 1564, 1732
ApoI RAATTY 2 cut(s) 425, 1588
ArsI GACNNNNNNTTYG 6 cut(s) 75, 107, 866, 898, 1625, 1657
AsiGI ACCGGT 1 cut(s) 1771
Asp700I GAANNNNTTC 3 cut(s) 926, 1130, 1835
AspLEI GCGC 4 cut(s) 324, 771, 834, 936
AspS9I GGNCC 9 cut(s) 23, 148, 271, 442, 554, 727, 994, 1062, 1420
AsuC2I CCSGG 5 cut(s) 331, 449, 730, 1351, 1779
AsuHPI GGTGA 5 cut(s) 835, 1262, 1450, 1591, 1730
AvaI CYCGRG 2 cut(s) 140, 1759
AvaII GGWCC 6 cut(s) 23, 271, 442, 554, 994, 1420
BaeGI GKGCMC 1 cut(s) 866
BalI TGGCCA 1 cut(s) 713
BanI GGYRCC 4 cut(s) 326, 861, 1018, 1774
BanII GRGCYC 4 cut(s) 36, 66, 141, 1213
BauI CACGAG 2 cut(s) 764, 1223
BbsI GAAGAC 2 cut(s) 518, 1187
Bbv12I GWGCWC 2 cut(s) 36, 741
BbvCI CCTCAGC 4 cut(s) 686, 1001, 1025, 1461
BbvI GCAGC 7 cut(s) 1029, 1189, 1203, 1383, 1452, 1551, 1744
BccI CCATC 3 cut(s) 121, 139, 1513
BceAI ACGGC 3 cut(s) 375, 973, 1319
BciT130I CCWGG 5 cut(s) 268, 848, 1426, 1791, 1841
BclI TGATCA 3 cut(s) 1440, 1608, 1645
BcnI CCSGG 5 cut(s) 331, 449, 730, 1351, 1779
BcoDI GTCTC 4 cut(s) 109, 184, 1242, 1664
BfaI CTAG 2 cut(s) 1241, 1913
BfuAI ACCTGC 2 cut(s) 1252, 1540
BglI GCCNNNNNGGC 1 cut(s) 719
BlpI GCTNAGC 3 cut(s) 35, 1412, 1568
BmcAI AGTACT 1 cut(s) 1166
Bme18I GGWCC 6 cut(s) 23, 271, 442, 554, 994, 1420
BmeRI GACNNNNNGTC 1 cut(s) 1762
BmeT110I CYCGRG 2 cut(s) 140, 1759
BmgBI CACGTC 5 cut(s) 217, 484, 1177, 1345, 1684
BmgT120I GGNCC 9 cut(s) 23, 148, 271, 442, 554, 727, 994, 1062, 1420
BmsI GCATC 1 cut(s) 921
BpiI GAAGAC 2 cut(s) 518, 1187
BpmI CTGGAG 2 cut(s) 12, 1058
Bpu10I CCTNAGC 4 cut(s) 686, 1001, 1025, 1461
Bpu1102I GCTNAGC 3 cut(s) 35, 1412, 1568
BpuEI CTTGAG 1 cut(s) 278
BpuMI CCSGG 5 cut(s) 331, 449, 730, 1351, 1779
Bsa29I ATCGAT 1 cut(s) 125
BsaHI GRCGYC 1 cut(s) 1100
BsaI GGTCTC 2 cut(s) 109, 1242
BsaJI CCNNGG 8 cut(s) 87, 267, 313, 708, 846, 1424, 1778, 1790
BsaWI WCCGGW 5 cut(s) 223, 745, 1597, 1771, 1864
BsaXI ACNNNNNCTCC 4 cut(s) 1286, 1316, 1697, 1727
Bse118I RCCGGY 5 cut(s) 77, 185, 462, 996, 1771
Bse1I ACTGG 1 cut(s) 1504
BseAI TCCGGA 2 cut(s) 223, 745
BseBI CCWGG 5 cut(s) 268, 848, 1426, 1791, 1841
BseCI ATCGAT 1 cut(s) 125
BseDI CCNNGG 8 cut(s) 87, 267, 313, 708, 846, 1424, 1778, 1790
BseGI GGATG 4 cut(s) 56, 1489, 1536, 1873
BseNI ACTGG 1 cut(s) 1504
BseRI GAGGAG 1 cut(s) 868
BseSI GKGCMC 1 cut(s) 866
BseXI GCAGC 7 cut(s) 1029, 1189, 1203, 1383, 1452, 1551, 1744
BseYI CCCAGC 1 cut(s) 1665
BsgI GTGCAG 1 cut(s) 746
Bsh1236I CGCG 6 cut(s) 324, 917, 934, 936, 980, 1724
Bsh1285I CGRYCG 2 cut(s) 873, 1865
BshFI GGCC 7 cut(s) 150, 266, 713, 728, 803, 1064, 1334
BshNI GGYRCC 4 cut(s) 326, 861, 1018, 1774
BshTI ACCGGT 1 cut(s) 1771
BshVI ATCGAT 1 cut(s) 125
BsiEI CGRYCG 2 cut(s) 873, 1865
BsiHKAI GWGCWC 2 cut(s) 36, 741
BsiHKCI CYCGRG 2 cut(s) 140, 1759
BslFI GGGAC 6 cut(s) 284, 567, 1007, 1259, 1378, 1769
BsmAI GTCTC 4 cut(s) 109, 184, 1242, 1664
BsmBI CGTCTC 2 cut(s) 184, 1664
BsmFI GGGAC 6 cut(s) 284, 567, 1007, 1259, 1378, 1769
BsnI GGCC 7 cut(s) 150, 266, 713, 728, 803, 1064, 1334
Bso31I GGTCTC 2 cut(s) 109, 1242
BsoBI CYCGRG 2 cut(s) 140, 1759
Bsp1286I GDGCHC 6 cut(s) 36, 66, 141, 741, 866, 1213
Bsp13I TCCGGA 2 cut(s) 223, 745
Bsp1407I TGTACA 2 cut(s) 1053, 1086
Bsp1720I GCTNAGC 3 cut(s) 35, 1412, 1568
Bsp19I CCATGG 1 cut(s) 708
Bsp68I TCGCGA 1 cut(s) 917
BspANI GGCC 7 cut(s) 150, 266, 713, 728, 803, 1064, 1334
BspDI ATCGAT 1 cut(s) 125
BspEI TCCGGA 2 cut(s) 223, 745
BspFNI CGCG 6 cut(s) 324, 917, 934, 936, 980, 1724
BspMI ACCTGC 2 cut(s) 1252, 1540
BspPI GGATC 2 cut(s) 737, 1278
BspQI GCTCTTC 1 cut(s) 1143
BspT107I GGYRCC 4 cut(s) 326, 861, 1018, 1774
BspTNI GGTCTC 2 cut(s) 109, 1242
BsrBI CCGCTC 2 cut(s) 1153, 1710
BsrFI RCCGGY 5 cut(s) 77, 185, 462, 996, 1771
BsrGI TGTACA 2 cut(s) 1053, 1086
BsrI ACTGG 1 cut(s) 1504
BssAI RCCGGY 5 cut(s) 77, 185, 462, 996, 1771
BssECI CCNNGG 8 cut(s) 87, 267, 313, 708, 846, 1424, 1778, 1790
BssNI GRCGYC 1 cut(s) 1100
BssSI CACGAG 2 cut(s) 764, 1223
BssT1I CCWWGG 2 cut(s) 87, 708
Bst2BI CACGAG 2 cut(s) 764, 1223
Bst2UI CCWGG 5 cut(s) 268, 848, 1426, 1791, 1841
Bst4CI ACNGT 2 cut(s) 558, 1250
Bst6I CTCTTC 5 cut(s) 212, 609, 1143, 1277, 1893
BstACI GRCGYC 1 cut(s) 1100
BstAPI GCANNNNNTGC 1 cut(s) 195
BstAUI TGTACA 2 cut(s) 1053, 1086
BstC8I GCNNGC 4 cut(s) 320, 403, 679, 683
BstDSI CCRYGG 1 cut(s) 708
BstF5I GGATG 4 cut(s) 56, 1489, 1536, 1873
BstFNI CGCG 6 cut(s) 324, 917, 934, 936, 980, 1724
BstHHI GCGC 4 cut(s) 324, 771, 834, 936
BstMAI GTCTC 4 cut(s) 109, 184, 1242, 1664
BstMCI CGRYCG 2 cut(s) 873, 1865
BstMWI GCNNNNNNNGC 9 cut(s) 195, 324, 379, 719, 977, 1021, 1106, 1199, 1208
BstNI CCWGG 5 cut(s) 268, 848, 1426, 1791, 1841
BstNSI RCATGY 1 cut(s) 1303
BstSLI GKGCMC 1 cut(s) 866
BstUI CGCG 6 cut(s) 324, 917, 934, 936, 980, 1724
BstV1I GCAGC 7 cut(s) 1029, 1189, 1203, 1383, 1452, 1551, 1744
BstV2I GAAGAC 2 cut(s) 518, 1187
BstX2I RGATCY 1 cut(s) 1270
BstYI RGATCY 1 cut(s) 1270
Bsu15I ATCGAT 1 cut(s) 125
BsuRI GGCC 7 cut(s) 150, 266, 713, 728, 803, 1064, 1334
BsuTUI ATCGAT 1 cut(s) 125
BtgI CCRYGG 1 cut(s) 708
BtgZI GCGATG 2 cut(s) 898, 1124
BtrI CACGTC 5 cut(s) 217, 484, 1177, 1345, 1684
BtsCI GGATG 4 cut(s) 56, 1489, 1536, 1873
BtsIMutI CAGTG 2 cut(s) 1283, 1456
BtuMI TCGCGA 1 cut(s) 917
BveI ACCTGC 2 cut(s) 1252, 1540
Cac8I GCNNGC 4 cut(s) 320, 403, 679, 683
CfoI GCGC 4 cut(s) 324, 771, 834, 936
Cfr10I RCCGGY 5 cut(s) 77, 185, 462, 996, 1771
Cfr13I GGNCC 9 cut(s) 23, 148, 271, 442, 554, 727, 994, 1062, 1420
ClaI ATCGAT 1 cut(s) 125
CseI GACGC 2 cut(s) 1089, 1730
Csp6I GTAC 7 cut(s) 733, 943, 982, 1054, 1087, 1165, 1769
CspAI ACCGGT 1 cut(s) 1771
CviAII CATG 7 cut(s) 709, 1057, 1142, 1300, 1357, 1636, 1876
CviQI GTAC 7 cut(s) 733, 943, 982, 1054, 1087, 1165, 1769
DrdI GACNNNNNNGTC 1 cut(s) 101
DriI GACNNNNNGTC 1 cut(s) 1762
DseDI GACNNNNNNGTC 1 cut(s) 101
EaeI YGGCCR 2 cut(s) 711, 1332
Eam1104I CTCTTC 5 cut(s) 212, 609, 1143, 1277, 1893
Eam1105I GACNNNNNGTC 1 cut(s) 1762
EarI CTCTTC 5 cut(s) 212, 609, 1143, 1277, 1893
EciI GGCGGA 2 cut(s) 157, 1283
Ecl136II GAGCTC 1 cut(s) 34
Eco130I CCWWGG 2 cut(s) 87, 708
Eco147I AGGCCT 1 cut(s) 266
Eco24I GRGCYC 4 cut(s) 36, 66, 141, 1213
Eco31I GGTCTC 2 cut(s) 109, 1242
Eco32I GATATC 1 cut(s) 1890
Eco47I GGWCC 6 cut(s) 23, 271, 442, 554, 994, 1420
Eco53kI GAGCTC 1 cut(s) 34
Eco57I CTGAAG 3 cut(s) 818, 1259, 1850
Eco88I CYCGRG 2 cut(s) 140, 1759
EcoICRI GAGCTC 1 cut(s) 34
EcoO109I RGGNCCY 2 cut(s) 442, 1420
EcoRI GAATTC 1 cut(s) 425
EcoRII CCWGG 5 cut(s) 266, 846, 1424, 1789, 1839
EcoRV GATATC 1 cut(s) 1890
EcoT14I CCWWGG 2 cut(s) 87, 708
EcoT38I GRGCYC 4 cut(s) 36, 66, 141, 1213
ErhI CCWWGG 2 cut(s) 87, 708
Esp3I CGTCTC 2 cut(s) 184, 1664
FaeI CATG 7 cut(s) 712, 1060, 1145, 1303, 1360, 1639, 1879
FaqI GGGAC 6 cut(s) 284, 567, 1007, 1259, 1378, 1769
FatI CATG 7 cut(s) 708, 1056, 1141, 1299, 1356, 1635, 1875
FauI CCCGC 3 cut(s) 241, 939, 1703
FbaI TGATCA 3 cut(s) 1440, 1608, 1645
FblI GTMKAC 1 cut(s) 93
FokI GGATG 4 cut(s) 43, 1496, 1543, 1880
FriOI GRGCYC 4 cut(s) 36, 66, 141, 1213
FspBI CTAG 2 cut(s) 1241, 1913
GlaI GCGC 4 cut(s) 323, 770, 833, 935
GsaI CCCAGC 1 cut(s) 1669
GsuI CTGGAG 2 cut(s) 12, 1058
HaeIII GGCC 7 cut(s) 150, 266, 713, 728, 803, 1064, 1334
HgaI GACGC 2 cut(s) 1089, 1730
HhaI GCGC 4 cut(s) 324, 771, 834, 936
Hin1I GRCGYC 1 cut(s) 1100
Hin1II CATG 7 cut(s) 712, 1060, 1145, 1303, 1360, 1639, 1879
Hin6I GCGC 4 cut(s) 322, 769, 832, 934
HinP1I GCGC 4 cut(s) 322, 769, 832, 934
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HindIII AAGCTT 1 cut(s) 296
HphI GGTGA 5 cut(s) 835, 1262, 1450, 1591, 1730
Hpy166II GTNNAC 2 cut(s) 94, 1054
Hpy8I GTNNAC 2 cut(s) 94, 1054
Hpy99I CGWCG 3 cut(s) 221, 959, 1105
HpyCH4III ACNGT 2 cut(s) 558, 1250
HpyCH4IV ACGT 6 cut(s) 177, 216, 483, 1176, 1344, 1683
HpyCH4V TGCA 4 cut(s) 306, 405, 763, 1468
HpyF10VI GCNNNNNNNGC 9 cut(s) 195, 324, 379, 719, 977, 1021, 1106, 1199, 1208
HpySE526I ACGT 6 cut(s) 177, 216, 483, 1176, 1344, 1683
Hsp92I GRCGYC 1 cut(s) 1100
Hsp92II CATG 7 cut(s) 712, 1060, 1145, 1303, 1360, 1639, 1879
HspAI GCGC 4 cut(s) 322, 769, 832, 934
Kpn2I TCCGGA 2 cut(s) 223, 745
Ksp22I TGATCA 3 cut(s) 1440, 1608, 1645
LguI GCTCTTC 1 cut(s) 1143
LmnI GCTCC 5 cut(s) 10, 378, 1039, 1208, 1353
Lsp1109I GCAGC 7 cut(s) 1029, 1189, 1203, 1383, 1452, 1551, 1744
LweI GCATC 1 cut(s) 921
MaeI CTAG 2 cut(s) 1241, 1913
MaeII ACGT 6 cut(s) 177, 216, 483, 1176, 1344, 1683
MaeIII GTNAC 4 cut(s) 892, 1340, 1631, 1718
MbiI CCGCTC 2 cut(s) 1153, 1710
MflI RGATCY 1 cut(s) 1270
MhlI GDGCHC 6 cut(s) 36, 66, 141, 741, 866, 1213
MlsI TGGCCA 1 cut(s) 713
MluCI AATT 6 cut(s) 425, 659, 1080, 1117, 1588, 1880
MluI ACGCGT 1 cut(s) 978
MluNI TGGCCA 1 cut(s) 713
MlyI GAGTC 3 cut(s) 869, 1360, 1751
MmeI TCCRAC 2 cut(s) 363, 511
Mox20I TGGCCA 1 cut(s) 713
MroI TCCGGA 2 cut(s) 223, 745
MroXI GAANNNNTTC 3 cut(s) 926, 1130, 1835
MscI TGGCCA 1 cut(s) 713
Msp20I TGGCCA 1 cut(s) 713
MspA1I CMGCKG 4 cut(s) 1024, 1465, 1665, 1735
MvaI CCWGG 5 cut(s) 268, 848, 1426, 1791, 1841
MvnI CGCG 6 cut(s) 324, 917, 934, 936, 980, 1724
MwoI GCNNNNNNNGC 9 cut(s) 195, 324, 379, 719, 977, 1021, 1106, 1199, 1208
NciI CCSGG 5 cut(s) 331, 449, 730, 1351, 1779
NcoI CCATGG 1 cut(s) 708
NlaIII CATG 7 cut(s) 712, 1060, 1145, 1303, 1360, 1639, 1879
NmeAIII GCCGAG 1 cut(s) 814
NmuCI GTSAC 4 cut(s) 892, 1340, 1631, 1718
NruI TCGCGA 1 cut(s) 917
NspI RCATGY 1 cut(s) 1303
PaeR7I CTCGAG 1 cut(s) 1759
PaqCI CACCTGC 2 cut(s) 1252, 1540
PceI AGGCCT 1 cut(s) 266
PciI ACATGT 1 cut(s) 1299
PciSI GCTCTTC 1 cut(s) 1143
PcsI WCGNNNNNNNCGW 1 cut(s) 1190
PdmI GAANNNNTTC 3 cut(s) 926, 1130, 1835
PflMI CCANNNNNTGG 2 cut(s) 1071, 1665
PfoI TCCNGGA 3 cut(s) 447, 1349, 1839
PinAI ACCGGT 1 cut(s) 1771
Ple19I CGATCG 1 cut(s) 873
PleI GAGTC 3 cut(s) 869, 1360, 1751
PpsI GAGTC 3 cut(s) 869, 1360, 1751
PpuMI RGGWCCY 2 cut(s) 442, 1420
PscI ACATGT 1 cut(s) 1299
Psp124BI GAGCTC 1 cut(s) 36
Psp5II RGGWCCY 2 cut(s) 442, 1420
Psp6I CCWGG 5 cut(s) 266, 846, 1424, 1789, 1839
PspFI CCCAGC 1 cut(s) 1665
PspGI CCWGG 5 cut(s) 266, 846, 1424, 1789, 1839
PspPI GGNCC 9 cut(s) 23, 148, 271, 442, 554, 727, 994, 1062, 1420
PspPPI RGGWCCY 2 cut(s) 442, 1420
PspXI VCTCGAGB 1 cut(s) 1759
PsuI RGATCY 1 cut(s) 1270
PvuI CGATCG 1 cut(s) 873
PvuII CAGCTG 3 cut(s) 1465, 1665, 1735
RruI TCGCGA 1 cut(s) 917
RsaI GTAC 7 cut(s) 734, 944, 983, 1055, 1088, 1166, 1770
RsaNI GTAC 7 cut(s) 733, 943, 982, 1054, 1087, 1165, 1769
SacI GAGCTC 1 cut(s) 36
SalI GTCGAC 1 cut(s) 92
SapI GCTCTTC 1 cut(s) 1143
Sau96I GGNCC 9 cut(s) 23, 148, 271, 442, 554, 727, 994, 1062, 1420
ScaI AGTACT 1 cut(s) 1166
SchI GAGTC 3 cut(s) 869, 1360, 1751
SduI GDGCHC 6 cut(s) 36, 66, 141, 741, 866, 1213
SfaNI GCATC 1 cut(s) 921
Sfr274I CTCGAG 1 cut(s) 1759
SgrAI CRCCGGYG 1 cut(s) 77
SinI GGWCC 6 cut(s) 23, 271, 442, 554, 994, 1420
SlaI CTCGAG 1 cut(s) 1759
SmlI CTYRAG 2 cut(s) 293, 1759
SmoI CTYRAG 2 cut(s) 293, 1759
Sse9I AATT 6 cut(s) 425, 659, 1080, 1117, 1588, 1880
SseBI AGGCCT 1 cut(s) 266
SspMI CTAG 2 cut(s) 1241, 1913
SstI GAGCTC 1 cut(s) 36
StuI AGGCCT 1 cut(s) 266
StyI CCWWGG 2 cut(s) 87, 708
TaaI ACNGT 2 cut(s) 558, 1250
TaiI ACGT 6 cut(s) 180, 219, 486, 1179, 1347, 1686
TaqII GACCGA 1 cut(s) 11
TasI AATT 6 cut(s) 425, 659, 1080, 1117, 1588, 1880
TatI WGTACW 3 cut(s) 1053, 1086, 1164
TauI GCSGC 4 cut(s) 327, 365, 854, 1024
TscAI CASTG 2 cut(s) 1283, 1456
TseFI GTSAC 4 cut(s) 892, 1340, 1631, 1718
TseI GCWGC 7 cut(s) 1042, 1202, 1216, 1396, 1465, 1564, 1732
Tsp45I GTSAC 4 cut(s) 892, 1340, 1631, 1718
TspDTI ATGAA 4 cut(s) 1127, 1334, 1547, 1864
TspGWI ACGGA 3 cut(s) 170, 626, 1688
TspRI CASTG 2 cut(s) 1283, 1456
Van91I CCANNNNNTGG 2 cut(s) 1071, 1665
VpaK11BI GGWCC 6 cut(s) 23, 271, 442, 554, 994, 1420
XapI RAATTY 2 cut(s) 425, 1588
XceI RCATGY 1 cut(s) 1303
XcmI CCANNNNNNNNNTGG 2 cut(s) 721, 1229
XhoI CTCGAG 1 cut(s) 1759
XmiI GTMKAC 1 cut(s) 93
XmnI GAANNNNTTC 3 cut(s) 926, 1130, 1835
XspI CTAG 2 cut(s) 1241, 1913
ZrmI AGTACT 1 cut(s) 1166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.