Domain of unknown function (DUF2828)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 471 |
| AarI | CACCTGC | 1 cut(s) | 68 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 242 |
| AatII | GACGTC | 1 cut(s) | 246 |
| Acc36I | ACCTGC | 1 cut(s) | 68 |
| AciI | CCGC | 1 cut(s) | 796 |
| AclI | AACGTT | 1 cut(s) | 596 |
| AclWI | GGATC | 2 cut(s) | 398, 561 |
| AcoI | YGGCCR | 2 cut(s) | 741, 780 |
| AcsI | RAATTY | 4 cut(s) | 21, 199, 248, 685 |
| AcuI | CTGAAG | 1 cut(s) | 298 |
| AcyI | GRCGYC | 1 cut(s) | 243 |
| AdeI | CACNNNGTG | 1 cut(s) | 967 |
| AfaI | GTAC | 1 cut(s) | 183 |
| AfeI | AGCGCT | 1 cut(s) | 1078 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 832 |
| AflII | CTTAAG | 1 cut(s) | 755 |
| AgsI | TTSAA | 3 cut(s) | 122, 766, 988 |
| AjnI | CCWGG | 1 cut(s) | 99 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 752, 784 |
| AluBI | AGCT | 4 cut(s) | 67, 522, 617, 875 |
| AluI | AGCT | 4 cut(s) | 67, 522, 617, 875 |
| Alw21I | GWGCWC | 2 cut(s) | 681, 1052 |
| Alw26I | GTCTC | 3 cut(s) | 272, 323, 652 |
| AlwI | GGATC | 2 cut(s) | 398, 561 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 806, 875 |
| Ama87I | CYCGRG | 1 cut(s) | 275 |
| Aor51HI | AGCGCT | 1 cut(s) | 1078 |
| AoxI | GGCC | 3 cut(s) | 49, 741, 780 |
| ApeKI | GCWGC | 2 cut(s) | 773, 806 |
| ApoI | RAATTY | 4 cut(s) | 21, 199, 248, 685 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 117 |
| AspLEI | GCGC | 3 cut(s) | 778, 947, 1079 |
| AsuHPI | GGTGA | 4 cut(s) | 52, 92, 223, 1019 |
| AsuII | TTCGAA | 1 cut(s) | 213 |
| AvaI | CYCGRG | 1 cut(s) | 275 |
| BalI | TGGCCA | 1 cut(s) | 782 |
| BanII | GRGCYC | 1 cut(s) | 490 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 382, 414 |
| BauI | CACGAG | 1 cut(s) | 1061 |
| BbsI | GAAGAC | 3 cut(s) | 146, 923, 1063 |
| Bbv12I | GWGCWC | 2 cut(s) | 681, 1052 |
| BbvI | GCAGC | 2 cut(s) | 760, 793 |
| BccI | CCATC | 1 cut(s) | 1063 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 904, 938 |
| BciT130I | CCWGG | 1 cut(s) | 101 |
| BcoDI | GTCTC | 3 cut(s) | 272, 323, 652 |
| BfaI | CTAG | 4 cut(s) | 189, 335, 614, 1053 |
| BfoI | RGCGCY | 1 cut(s) | 1080 |
| BfrI | CTTAAG | 1 cut(s) | 755 |
| BfuAI | ACCTGC | 1 cut(s) | 68 |
| BisI | GCNGC | 2 cut(s) | 774, 807 |
| BlpI | GCTNAGC | 1 cut(s) | 68 |
| BlsI | GCNGC | 2 cut(s) | 775, 808 |
| Bme1390I | CCNGG | 1 cut(s) | 101 |
| BmeT110I | CYCGRG | 1 cut(s) | 275 |
| BmrFI | CCNGG | 1 cut(s) | 101 |
| BmsI | GCATC | 4 cut(s) | 934, 956, 965, 1029 |
| BpiI | GAAGAC | 3 cut(s) | 146, 923, 1063 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 68 |
| Bpu14I | TTCGAA | 1 cut(s) | 213 |
| BpuEI | CTTGAG | 1 cut(s) | 634 |
| BsaHI | GRCGYC | 1 cut(s) | 243 |
| BsaI | GGTCTC | 2 cut(s) | 272, 652 |
| BsaJI | CCNNGG | 2 cut(s) | 99, 369 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 832 |
| Bse3DI | GCAATG | 1 cut(s) | 674 |
| BseBI | CCWGG | 1 cut(s) | 101 |
| BseDI | CCNNGG | 2 cut(s) | 99, 369 |
| BseGI | GGATG | 2 cut(s) | 980, 1074 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 832 |
| BseMI | GCAATG | 1 cut(s) | 674 |
| BseMII | CTCAG | 2 cut(s) | 82, 830 |
| BseRI | GAGGAG | 1 cut(s) | 1025 |
| BseXI | GCAGC | 2 cut(s) | 760, 793 |
| BseYI | CCCAGC | 1 cut(s) | 970 |
| BshFI | GGCC | 3 cut(s) | 51, 743, 782 |
| BsiHKAI | GWGCWC | 2 cut(s) | 681, 1052 |
| BsiHKCI | CYCGRG | 1 cut(s) | 275 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 832 |
| BsmAI | GTCTC | 3 cut(s) | 272, 323, 652 |
| BsnI | GGCC | 3 cut(s) | 51, 743, 782 |
| Bso31I | GGTCTC | 2 cut(s) | 272, 652 |
| BsoBI | CYCGRG | 1 cut(s) | 275 |
| Bsp119I | TTCGAA | 1 cut(s) | 213 |
| Bsp1286I | GDGCHC | 3 cut(s) | 490, 681, 1052 |
| Bsp143I | GATC | 4 cut(s) | 140, 403, 553, 711 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 68 |
| BspACI | CCGC | 1 cut(s) | 796 |
| BspANI | GGCC | 3 cut(s) | 51, 743, 782 |
| BspCNI | CTCAG | 2 cut(s) | 81, 829 |
| BspMI | ACCTGC | 1 cut(s) | 68 |
| BspPI | GGATC | 2 cut(s) | 398, 561 |
| BspT104I | TTCGAA | 1 cut(s) | 213 |
| BspTI | CTTAAG | 1 cut(s) | 755 |
| BspTNI | GGTCTC | 2 cut(s) | 272, 652 |
| BsrDI | GCAATG | 1 cut(s) | 674 |
| BssECI | CCNNGG | 2 cut(s) | 99, 369 |
| BssMI | GATC | 4 cut(s) | 140, 403, 553, 711 |
| BssNI | GRCGYC | 1 cut(s) | 243 |
| BssSI | CACGAG | 1 cut(s) | 1061 |
| BssT1I | CCWWGG | 1 cut(s) | 369 |
| Bst2BI | CACGAG | 1 cut(s) | 1061 |
| Bst2UI | CCWGG | 1 cut(s) | 101 |
| Bst4CI | ACNGT | 3 cut(s) | 698, 789, 802 |
| BstACI | GRCGYC | 1 cut(s) | 243 |
| BstAFI | CTTAAG | 1 cut(s) | 755 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 664 |
| BstBI | TTCGAA | 1 cut(s) | 213 |
| BstC8I | GCNNGC | 1 cut(s) | 780 |
| BstDEI | CTNAG | 4 cut(s) | 68, 253, 518, 816 |
| BstF5I | GGATG | 2 cut(s) | 980, 1074 |
| BstH2I | RGCGCY | 1 cut(s) | 1080 |
| BstHHI | GCGC | 3 cut(s) | 778, 947, 1079 |
| BstKTI | GATC | 4 cut(s) | 143, 406, 556, 714 |
| BstMAI | GTCTC | 3 cut(s) | 272, 323, 652 |
| BstMBI | GATC | 4 cut(s) | 140, 403, 553, 711 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 494, 664, 740, 779, 812, 1039 |
| BstNI | CCWGG | 1 cut(s) | 101 |
| BstNSI | RCATGY | 1 cut(s) | 267 |
| BstSCI | CCNGG | 1 cut(s) | 99 |
| BstV1I | GCAGC | 2 cut(s) | 760, 793 |
| BstV2I | GAAGAC | 3 cut(s) | 146, 923, 1063 |
| BsuRI | GGCC | 3 cut(s) | 51, 743, 782 |
| BtsCI | GGATG | 2 cut(s) | 980, 1074 |
| BtsIMutI | CAGTG | 1 cut(s) | 714 |
| BveI | ACCTGC | 1 cut(s) | 68 |
| Cac8I | GCNNGC | 1 cut(s) | 780 |
| CaiI | CAGNNNCTG | 2 cut(s) | 806, 875 |
| CfoI | GCGC | 3 cut(s) | 778, 947, 1079 |
| CseI | GACGC | 2 cut(s) | 83, 245 |
| Csp6I | GTAC | 1 cut(s) | 182 |
| CviAII | CATG | 3 cut(s) | 264, 899, 1004 |
| CviQI | GTAC | 1 cut(s) | 182 |
| DdeI | CTNAG | 4 cut(s) | 68, 253, 518, 816 |
| DpnI | GATC | 4 cut(s) | 142, 405, 555, 713 |
| DpnII | GATC | 4 cut(s) | 140, 403, 553, 711 |
| DraIII | CACNNNGTG | 1 cut(s) | 967 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 242 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 242 |
| EaeI | YGGCCR | 2 cut(s) | 741, 780 |
| Eco130I | CCWWGG | 1 cut(s) | 369 |
| Eco24I | GRGCYC | 1 cut(s) | 490 |
| Eco31I | GGTCTC | 2 cut(s) | 272, 652 |
| Eco47III | AGCGCT | 1 cut(s) | 1078 |
| Eco57I | CTGAAG | 1 cut(s) | 298 |
| Eco88I | CYCGRG | 1 cut(s) | 275 |
| EcoRI | GAATTC | 2 cut(s) | 21, 248 |
| EcoRII | CCWGG | 1 cut(s) | 99 |
| EcoT14I | CCWWGG | 1 cut(s) | 369 |
| EcoT38I | GRGCYC | 1 cut(s) | 490 |
| ErhI | CCWWGG | 1 cut(s) | 369 |
| FaeI | CATG | 3 cut(s) | 267, 902, 1007 |
| FaiI | YATR | 9 cut(s) | 265, 306, 425, 471, 708, 848, 900, 995, 1005 |
| FatI | CATG | 3 cut(s) | 263, 898, 1003 |
| Fnu4HI | GCNGC | 2 cut(s) | 774, 807 |
| FokI | GGATG | 2 cut(s) | 987, 1081 |
| FriOI | GRGCYC | 1 cut(s) | 490 |
| Fsp4HI | GCNGC | 2 cut(s) | 774, 807 |
| FspBI | CTAG | 4 cut(s) | 189, 335, 614, 1053 |
| GlaI | GCGC | 3 cut(s) | 777, 946, 1078 |
| GluI | GCNGC | 2 cut(s) | 774, 807 |
| GsaI | CCCAGC | 1 cut(s) | 974 |
| HaeII | RGCGCY | 1 cut(s) | 1080 |
| HaeIII | GGCC | 3 cut(s) | 51, 743, 782 |
| HgaI | GACGC | 2 cut(s) | 83, 245 |
| HhaI | GCGC | 3 cut(s) | 778, 947, 1079 |
| Hin1I | GRCGYC | 1 cut(s) | 243 |
| Hin1II | CATG | 3 cut(s) | 267, 902, 1007 |
| Hin6I | GCGC | 3 cut(s) | 776, 945, 1077 |
| HinP1I | GCGC | 3 cut(s) | 776, 945, 1077 |
| HincII | GTYRAC | 1 cut(s) | 867 |
| HindII | GTYRAC | 1 cut(s) | 867 |
| HinfI | GANTC | 3 cut(s) | 192, 536, 1059 |
| HpaI | GTTAAC | 1 cut(s) | 867 |
| HphI | GGTGA | 4 cut(s) | 52, 92, 223, 1019 |
| Hpy166II | GTNNAC | 2 cut(s) | 515, 867 |
| Hpy188I | TCNGA | 5 cut(s) | 161, 223, 481, 819, 952 |
| Hpy188III | TCNNGA | 3 cut(s) | 38, 277, 1063 |
| Hpy8I | GTNNAC | 2 cut(s) | 515, 867 |
| Hpy99I | CGWCG | 3 cut(s) | 77, 239, 248 |
| HpyAV | CCTTC | 3 cut(s) | 103, 322, 384 |
| HpyCH4III | ACNGT | 3 cut(s) | 698, 789, 802 |
| HpyCH4IV | ACGT | 3 cut(s) | 243, 296, 596 |
| HpyCH4V | TGCA | 7 cut(s) | 108, 575, 658, 667, 887, 925, 1003 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 494, 664, 740, 779, 812, 1039 |
| HpyF3I | CTNAG | 4 cut(s) | 68, 253, 518, 816 |
| HpySE526I | ACGT | 3 cut(s) | 243, 296, 596 |
| Hsp92I | GRCGYC | 1 cut(s) | 243 |
| Hsp92II | CATG | 3 cut(s) | 267, 902, 1007 |
| HspAI | GCGC | 3 cut(s) | 776, 945, 1077 |
| KspAI | GTTAAC | 1 cut(s) | 867 |
| Kzo9I | GATC | 4 cut(s) | 140, 403, 553, 711 |
| LmnI | GCTCC | 2 cut(s) | 676, 872 |
| Lsp1109I | GCAGC | 2 cut(s) | 760, 793 |
| LweI | GCATC | 4 cut(s) | 934, 956, 965, 1029 |
| MaeI | CTAG | 4 cut(s) | 189, 335, 614, 1053 |
| MaeII | ACGT | 3 cut(s) | 243, 296, 596 |
| MaeIII | GTNAC | 3 cut(s) | 35, 80, 229 |
| MalI | GATC | 4 cut(s) | 142, 405, 555, 713 |
| MboI | GATC | 4 cut(s) | 140, 403, 553, 711 |
| MboII | GAAGA | 4 cut(s) | 53, 146, 923, 1068 |
| MfeI | CAATTG | 1 cut(s) | 498 |
| MhlI | GDGCHC | 3 cut(s) | 490, 681, 1052 |
| MlsI | TGGCCA | 1 cut(s) | 782 |
| MluNI | TGGCCA | 1 cut(s) | 782 |
| MlyI | GAGTC | 1 cut(s) | 1053 |
| MmeI | TCCRAC | 3 cut(s) | 184, 717, 816 |
| MnlI | CCTC | 9 cut(s) | 68, 121, 229, 455, 500, 696, 829, 1003, 1059 |
| Mox20I | TGGCCA | 1 cut(s) | 782 |
| MroXI | GAANNNNTTC | 1 cut(s) | 117 |
| MscI | TGGCCA | 1 cut(s) | 782 |
| MseI | TTAA | 5 cut(s) | 381, 599, 747, 756, 866 |
| Msp20I | TGGCCA | 1 cut(s) | 782 |
| MspCI | CTTAAG | 1 cut(s) | 755 |
| MspR9I | CCNGG | 1 cut(s) | 101 |
| MunI | CAATTG | 1 cut(s) | 498 |
| MvaI | CCWGG | 1 cut(s) | 101 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 494, 664, 740, 779, 812, 1039 |
| NdeII | GATC | 4 cut(s) | 140, 403, 553, 711 |
| NlaIII | CATG | 3 cut(s) | 267, 902, 1007 |
| NmeAIII | GCCGAG | 1 cut(s) | 719 |
| NmuCI | GTSAC | 3 cut(s) | 35, 80, 229 |
| NspI | RCATGY | 1 cut(s) | 267 |
| NspV | TTCGAA | 1 cut(s) | 213 |
| PaeR7I | CTCGAG | 1 cut(s) | 275 |
| PaqCI | CACCTGC | 1 cut(s) | 68 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 219, 243 |
| PdmI | GAANNNNTTC | 1 cut(s) | 117 |
| PfeI | GAWTC | 2 cut(s) | 192, 536 |
| PkrI | GCNGC | 2 cut(s) | 775, 808 |
| PleI | GAGTC | 1 cut(s) | 1053 |
| PpsI | GAGTC | 1 cut(s) | 1053 |
| PsiI | TTATAA | 1 cut(s) | 471 |
| Psp1406I | AACGTT | 1 cut(s) | 596 |
| Psp6I | CCWGG | 1 cut(s) | 99 |
| PspFI | CCCAGC | 1 cut(s) | 970 |
| PspGI | CCWGG | 1 cut(s) | 99 |
| PstNI | CAGNNNCTG | 2 cut(s) | 806, 875 |
| RsaI | GTAC | 1 cut(s) | 183 |
| RsaNI | GTAC | 1 cut(s) | 182 |
| SaqAI | TTAA | 5 cut(s) | 381, 599, 747, 756, 866 |
| SatI | GCNGC | 2 cut(s) | 774, 807 |
| Sau3AI | GATC | 4 cut(s) | 140, 403, 553, 711 |
| SchI | GAGTC | 1 cut(s) | 1053 |
| ScrFI | CCNGG | 1 cut(s) | 101 |
| SduI | GDGCHC | 3 cut(s) | 490, 681, 1052 |
| SfaNI | GCATC | 4 cut(s) | 934, 956, 965, 1029 |
| Sfr274I | CTCGAG | 1 cut(s) | 275 |
| SfuI | TTCGAA | 1 cut(s) | 213 |
| SlaI | CTCGAG | 1 cut(s) | 275 |
| SmlI | CTYRAG | 3 cut(s) | 275, 649, 755 |
| SmoI | CTYRAG | 3 cut(s) | 275, 649, 755 |
| SsiI | CCGC | 1 cut(s) | 796 |
| SspMI | CTAG | 4 cut(s) | 189, 335, 614, 1053 |
| StyD4I | CCNGG | 1 cut(s) | 99 |
| StyI | CCWWGG | 1 cut(s) | 369 |
| TaaI | ACNGT | 3 cut(s) | 698, 789, 802 |
| TaiI | ACGT | 3 cut(s) | 246, 299, 599 |
| TaqI | TCGA | 6 cut(s) | 126, 213, 246, 276, 590, 701 |
| TfiI | GAWTC | 2 cut(s) | 192, 536 |
| Tru1I | TTAA | 5 cut(s) | 381, 599, 747, 756, 866 |
| Tru9I | TTAA | 5 cut(s) | 381, 599, 747, 756, 866 |
| TscAI | CASTG | 1 cut(s) | 721 |
| TseFI | GTSAC | 3 cut(s) | 35, 80, 229 |
| TseI | GCWGC | 2 cut(s) | 773, 806 |
| Tsp45I | GTSAC | 3 cut(s) | 35, 80, 229 |
| TspDTI | ATGAA | 1 cut(s) | 923 |
| TspGWI | ACGGA | 3 cut(s) | 140, 164, 566 |
| TspRI | CASTG | 1 cut(s) | 721 |
| Vha464I | CTTAAG | 1 cut(s) | 755 |
| XapI | RAATTY | 4 cut(s) | 21, 199, 248, 685 |
| XceI | RCATGY | 1 cut(s) | 267 |
| XhoI | CTCGAG | 1 cut(s) | 275 |
| XmnI | GAANNNNTTC | 1 cut(s) | 117 |
| XspI | CTAG | 4 cut(s) | 189, 335, 614, 1053 |
| ZraI | GACGTC | 1 cut(s) | 244 |