Rroxscaffold_3G00269290

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000255
Physical Location & Seq
Forward (+)
62194693 .. 62222099
27407 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00269290.1

Sequence Viewer

Length: 2307 bp
ATGAAGCCACAAATCCTCTCAATTCTCAAAGGCACCAAACCAAACCAAAACCATGTCAACTTGGCAAGAACCCAAACTCCTACTCTTCTTCTCGGTCCTCCAGAGATCGGAACAGTACCCACCACCAATGCTCCACCACAAGTCAATTCCGGCGACCCCTTCATGGATTTCATGGTCTCCAATTACAACAACACCACGTCAACTTCCCCCCGACCCCCAATGGGTTTGACCGAGAACAGTTCGCCTACTTTTCTCACCTCCGGCAACCCCTGCCTTGATTTTTTCTTCCACGTGGTGCCTGACACCAAGCCTGAATATTTCAACCAACAGCTCCCGTTGGCTTGGTCCAACGATGCCGTAACCACTCTCAAGCTCATCTGCAACCTGCGAGGTGTACGTGGCACCGGAAAGTGTGACAAAGAAGGGTTCTACACAGCAGCGTATTGGCTCTACAACCACCACCCTAAAACCCTAGCTTACAATGTAGCGTCGGTTGCCGCTTTTGGGTATTTCAAAGATCTGCCGGAGATTCTATACCGCCTTCTAGAAGGCCAAGACATCAGGAAGACCCAAAAGGAAGAGTGGGAAAGGATCAAGCGCTATTGCTATAAGGGACCGGGTAAGGTAGACGGGTTAAGGTCACTAAAGCTTGGGCATTTAATGGGTCCGGCGGATCATCCACCTAGAGATGTTAAACTGCTGAGGCTTTGGGAGAAACTCATCGCCGTCAAATCTAACAAGAAAAAACTCGAGAAGGGGGAGTATAAAACTGTTCAAGCAGAATGGGCAGCGGATAGAGACGAGCTGAAAAGTGATGAAGCTAGAGAACTGATGCATCAAAAGATCATAGACAAGACCAAGAATGCCGTGGCTAGGTATGAGCAAGACTCGGATTTGAAGTTCCTATACGAACAAATTTGGGATGTGTTTGGGGATTGCTTGAAGTCCATGGAGGCGGACACGGAGTCTGAGAGGAACGAGATGTTCCGATTGAAAAAAGAAAAAGTCAGGGAATTGAGGTATGAAAGAATCTACGAAATGGCCAGGAAGGCTGTTGAGAGGTACCAGCGTGACCCGGACTACAGATTCTTGCACGAACGGGTTTCGGATCTTTTCGCAGAGTACTTGAAATCAGATATGGAGAATTTGAAGTCCAATCAGTACAAGAAGATTAGTCTTGCGGCGAAATGGTGTCCTTCTCTGGACTCGTCTTTTGATCGTGCTACTCTGCTCTGTGAGAGCATTGCCAGGAAGGTTTTCACCCGAGAATCGCATCCGGAATATGAAGGAATTGAGGACGCACATTATGCTTATAGAGTCCGAGACCGGCTGAGGAAGGATGTGTTGGTGCCATTGAGGATAGTGTTGGAGTTGCCGGAGGTTTATATGGGTGCTAATCTGTGGGGTGCCATTCCTTACAACAGAGTGGCCTCTGTGGCGATGAAGCTTTACAAGGAGAAGTTCTTGAAGCATGATGAGGAGAGGTTTAAGAAGTATTTGGAGGATGTGAAGGCTGGGAAGTCTACTATTGCGGCCGGTGCTCTGCTTCCACATGAGATCATATCCTCTCTGAACGATGAGGATGGTGGGGAAGTGGGTGAGCTTCAGTGGAAGAGAATGGTGGAGGACCTGGTGGAGCTTGGCAAGTTGAGGAATTGCATGGCTGTGTGTGACGTGTCCGGAAGCATGGAAGGGACTCCTATGGAGGTGTGTGTGGCTTTGGGGCTGTTGGTGTCGGAATTGAGTGAAGACCCATGGAAAGGAAAGCTGATCACTTTCAGTCAGAACCCTCAGATGCATTTGATTCAAGGCGATGATCTTAGCTCCAAGTGCGAGTTTGTGAGGAGGATGGAATGGGGGATGAACACTGATTTTCAAAAGGTGTTTGATCTGATTCTCCAAGTGGCTGTGAAGGGGAAGTTGAAGCCGGAGCAGATGATAAAGAGGGTGTTTGTGTTCAGCGACATGGAGTTTGATGAGGCTTCGGGGCATAGGGGATATGGATATGGGTATTATGGAAGGGAGGATAATGAGCCCCAGAGCTGGGAGACCAATTATGAGGTGATACAGAGAAAGTATAAGGAAAATGGGTATGGTGATGCGGTGCCTCAGATCGTGTTTTGGAATCTCAGGGACTCTGGGTCTACACCGGTGACCGAGTCACAAAATGGGGTGGCGCTGCTCAGTGGCTTCTCCAAGAATTTGCTGAAATTGTTCTTGGGTAAGGATGTGGTGACTCGCCCGGACCAAACCATGGAAGAGGCCATCTCTGGCAAAGAATATCAGAATTTAGTTGTAGTGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

768

Amino Acids

88.23

Weight (kDa)

6.35

Isoelectric Point (pI)

27.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2828 PF11443 77 - 316 4.7e-49 Domain of unknown function (DUF2828)
DUF2828 PF11443 323 - 550 7.1e-86 Domain of unknown function (DUF2828)
DUF7788 PF25043 552 - 758 1.7e-82 Domain of unknown function (DUF7788)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000255)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24780 AT5G13210 AT5G43390 AT5G43400
fragaria_vesca FvH4_4g23010 FvH4_5g12462 FvH4_5g12490 FvH4_5g12491 FvH4_5g20030 FvH4_5g20031 FvH4_5g21510 FvH4_6g30070
malus_domestica MD00G1095100.v1.1 MD05G1186800.v1.1 MD05G1259500.v1.1 MD05G1260200.v1.1 MD06G1159400.v1.1 MD06G1159500.v1.1 MD06G1162500.v1.1 MD06G1164600.v1.1 MD06G1187800.v1.1 MD06G1212600.v1.1 MD06G1212700.v1.1 MD13G1126800.v1.1 MD14G1168300.v1.1 MD14G1206600.v1.1 MD14G1223900.v1.1 MD14G1230800.v1.1 MD15G1240300.v1.1 MD15G1416800.v1.1 MD16G1127300.v1.1
prunus_persica Prupe.1G218700_v2.0.a1 Prupe.4G045100_v2.0.a1 Prupe.5G159300_v2.0.a1 Prupe.5G172200_v2.0.a1 Prupe.5G172300_v2.0.a1 Prupe.5G173200_v2.0.a1 Prupe.5G173300_v2.0.a1 Prupe.5G173400_v2.0.a1 Prupe.5G219000_v2.0.a1 Prupe.5G219100_v2.0.a1 Prupe.5G219200_v2.0.a1
pyrus_communis pycom05g24390 pycom06g19020 pycom10g15060 pycom10g24800 pycom13g11010 pycom16g10800 pycom16g10810 pycom17g22350 pycom17g22360
rosa_chinensis RchiOBHm_Chr2g0136111 RchiOBHm_Chr4g0429291 RchiOBHm_Chr7g0184981 RchiOBHm_Chr7g0184991 RchiOBHm_Chr7g0185001 RchiOBHm_Chr7g0204841
rosa_laevigata RLG00000003427 RLG00000004941 RLG00000004943 RLG00000004944 RLG00000004945 RLG00000004946 RLG00000007084 RLG00000013701 RLG00000019505 RLG00000019506
rosa_multiflora Rmu_co8294559.1_g000001 Rmu_co8375195.1_g000001 Rmu_co8450783.1_g000001 Rmu_co8519567.1_g000001 Rmu_sc0000054.1_g000008 Rmu_sc0001689.1_g000017 Rmu_sc0001801.1_g000027 Rmu_sc0002070.1_g000010 Rmu_sc0002180.1_g000022 Rmu_sc0002986.1_g000018 Rmu_sc0003221.1_g000027 Rmu_sc0005629.1_g000018 Rmu_sc0009898.1_g000002 Rmu_sc0018428.1_g000003 Rmu_sc0018428.1_g000005 Rmu_sc0022816.1_g000001 Rmu_sc0041781.1_g000001 Rmu_ssc0000289.1_g000007 Rmu_ssc0000289.1_g000008 Rmu_ssc0000289.1_g000010
rosa_roxburghii Rroxscaffold_2G00108460 Rroxscaffold_3G00252720 Rroxscaffold_3G00269290 Rroxscaffold_5G00349680 Rroxscaffold_5G00370900 Rroxscaffold_6G00415020
rosa_rugosa Rorug01G0043400 Rorug02G0328200 Rorug04G0234800 Rorug06G0465600 Rorug06G0465800 Rorug06G0465900 Rorug06G0466000 Rorug06G0466100 Rorug07G0086200 Rorug07G0086200
rosa_samantha Rh2BG386700 Rh2CG365900 Rh2DG402800 Rh4AG290400 Rh4BG296100 Rh4CG311900 Rh4DG293300 Rh7AG077000 Rh7AG077100 Rh7AG077200 Rh7AG216600 Rh7BG070200 Rh7BG070300 Rh7BG070400 Rh7BG213700 Rh7CG071400 Rh7CG071600 Rh7CG071700 Rh7CG071900 Rh7CG230100 Rh7DG070800 Rh7DG070900 Rh7DG224200 Rh7DG440100
rosa_wichuraiana Rw2G030970 Rw4G025150 Rw7G005840 Rw7G005850 Rw7G005860 Rw7G018770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 393
Acc65I GGTACC 1 cut(s) 1062
AccB1I GGYRCC 7 cut(s) 32, 295, 401, 1062, 1348, 1406, 2104
AccB7I CCANNNNNTGG 1 cut(s) 2254
AccI GTMKAC 3 cut(s) 627, 1523, 2144
AccIII TCCGGA 2 cut(s) 1276, 1679
AciI CCGC 8 cut(s) 498, 538, 671, 791, 956, 1181, 1532, 2102
AclWI GGATC 3 cut(s) 599, 681, 1116
AcoI YGGCCR 2 cut(s) 1041, 1533
AcsI RAATTY 4 cut(s) 915, 1144, 2200, 2287
AcuI CTGAAG 1 cut(s) 1589
AcvI CACGTG 1 cut(s) 292
AdeI CACNNNGTG 1 cut(s) 295
AfaI GTAC 5 cut(s) 117, 396, 1064, 1124, 1163
AfeI AGCGCT 1 cut(s) 599
AfiI CCNNNNNNNGG 9 cut(s) 107, 163, 221, 504, 873, 1760, 2043, 2044, 2254
AflIII ACRYGT 1 cut(s) 1674
AgeI ACCGGT 1 cut(s) 2149
AhdI GACNNNNNGTC 2 cut(s) 964, 2140
AjiI CACGTC 2 cut(s) 198, 1675
AjnI CCWGG 3 cut(s) 1043, 1247, 1629
AjuI GAANNNNNNNTTGG 4 cut(s) 1328, 1360, 2201, 2233
AloI GAACNNNNNNTCC 4 cut(s) 61, 93, 884, 916
Alw21I GWGCWC 1 cut(s) 1543
Alw26I GTCTC 4 cut(s) 181, 792, 1317, 2042
AlwI GGATC 3 cut(s) 599, 681, 1116
Ama87I CYCGRG 2 cut(s) 749, 1263
Aor13HI TCCGGA 2 cut(s) 1276, 1679
Aor51HI AGCGCT 1 cut(s) 599
AoxI GGCC 5 cut(s) 550, 1041, 1428, 1533, 2262
ApeKI GCWGC 3 cut(s) 437, 788, 2179
ApoI RAATTY 4 cut(s) 915, 1144, 2200, 2287
ArsI GACNNNNNNTTYG 4 cut(s) 1196, 1228, 1955, 1987
AsiGI ACCGGT 1 cut(s) 2149
Asp700I GAANNNNTTC 2 cut(s) 1256, 2213
Asp718I GGTACC 1 cut(s) 1062
AspLEI GCGC 2 cut(s) 600, 2179
AspS9I GGNCC 6 cut(s) 95, 345, 614, 665, 1627, 2245
AsuC2I CCSGG 3 cut(s) 618, 1076, 2243
AsuHPI GGTGA 7 cut(s) 247, 1252, 1610, 2074, 2108, 2164, 2245
AvaI CYCGRG 2 cut(s) 749, 1263
AvaII GGWCC 6 cut(s) 95, 345, 614, 665, 1627, 2245
BaeI ACNNNNGTAYC 2 cut(s) 1054, 1087
BalI TGGCCA 1 cut(s) 1043
BanI GGYRCC 7 cut(s) 32, 295, 401, 1062, 1348, 1406, 2104
BanII GRGCYC 1 cut(s) 2037
BarI GAAGNNNNNNTAC 2 cut(s) 890, 922
BbrPI CACGTG 1 cut(s) 292
BbsI GAAGAC 2 cut(s) 572, 1755
Bbv12I GWGCWC 1 cut(s) 1543
BbvCI CCTCAGC 2 cut(s) 701, 1331
BbvI GCAGC 3 cut(s) 449, 800, 2166
BccI CCATC 3 cut(s) 1577, 1843, 2273
BceAI ACGGC 3 cut(s) 341, 710, 851
BciT130I CCWGG 3 cut(s) 1045, 1249, 1631
BclI TGATCA 1 cut(s) 1770
BcnI CCSGG 3 cut(s) 618, 1076, 2243
BcoDI GTCTC 4 cut(s) 181, 792, 1317, 2042
BfaI CTAG 5 cut(s) 473, 545, 684, 822, 873
BfmI CTRYAG 1 cut(s) 1081
BfoI RGCGCY 2 cut(s) 601, 2180
BfuAI ACCTGC 1 cut(s) 393
BglI GCCNNNNNGGC 2 cut(s) 1049, 1436
BglII AGATCT 1 cut(s) 517
BisI GCNGC 6 cut(s) 438, 498, 789, 1182, 1533, 2180
BlsI GCNGC 6 cut(s) 439, 499, 790, 1183, 1534, 2181
BmcAI AGTACT 1 cut(s) 1124
Bme1390I CCNGG 6 cut(s) 618, 1045, 1076, 1249, 1631, 2243
Bme18I GGWCC 6 cut(s) 95, 345, 614, 665, 1627, 2245
BmeRI GACNNNNNGTC 2 cut(s) 964, 2140
BmeT110I CYCGRG 2 cut(s) 749, 1263
BmgBI CACGTC 2 cut(s) 198, 1675
BmgT120I GGNCC 6 cut(s) 95, 345, 614, 665, 1627, 2245
BmiI GGNNCC 9 cut(s) 34, 297, 403, 615, 666, 1064, 1350, 1408, 2106
BmrFI CCNGG 6 cut(s) 618, 1045, 1076, 1249, 1631, 2243
BmsI GCATC 6 cut(s) 343, 822, 844, 1282, 1785, 2089
BpiI GAAGAC 2 cut(s) 572, 1755
BplI GAGNNNNNCTC 4 cut(s) 872, 904, 2252, 2284
BpmI CTGGAG 1 cut(s) 84
Bpu10I CCTNAGC 2 cut(s) 701, 1331
BpuEI CTTGAG 1 cut(s) 353
BpuMI CCSGG 3 cut(s) 618, 1076, 2243
BsaAI YACGTR 2 cut(s) 292, 398
BsaI GGTCTC 3 cut(s) 181, 1317, 2042
BsaJI CCNNGG 4 cut(s) 867, 948, 1754, 2253
BsaWI WCCGGW 4 cut(s) 404, 1276, 1679, 2149
BsaXI ACNNNNNCTCC 4 cut(s) 61, 91, 115, 145
Bsc4I CCNNNNNNNGG 9 cut(s) 107, 163, 221, 504, 873, 1760, 2043, 2044, 2254
Bse118I RCCGGY 3 cut(s) 1326, 1535, 2149
Bse3DI GCAATG 1 cut(s) 1242
BseAI TCCGGA 2 cut(s) 1276, 1679
BseBI CCWGG 3 cut(s) 1045, 1249, 1631
BseDI CCNNGG 4 cut(s) 867, 948, 1754, 2253
BseGI GGATG 9 cut(s) 676, 928, 1273, 1345, 1510, 1588, 1854, 1866, 2233
BseLI CCNNNNNNNGG 9 cut(s) 107, 163, 221, 504, 873, 1760, 2043, 2044, 2254
BseMI GCAATG 1 cut(s) 1242
BseMII CTCAG 7 cut(s) 692, 960, 1322, 1805, 2123, 2143, 2197
BseRI GAGGAG 2 cut(s) 1493, 1858
BseX3I CGGCCG 1 cut(s) 1533
BseXI GCAGC 3 cut(s) 449, 800, 2166
BseYI CCCAGC 2 cut(s) 1514, 2043
Bsh1285I CGRYCG 1 cut(s) 1536
BshFI GGCC 5 cut(s) 552, 1043, 1430, 1535, 2264
BshNI GGYRCC 7 cut(s) 32, 295, 401, 1062, 1348, 1406, 2104
BshTI ACCGGT 1 cut(s) 2149
BsiEI CGRYCG 1 cut(s) 1536
BsiHKAI GWGCWC 1 cut(s) 1543
BsiHKCI CYCGRG 2 cut(s) 749, 1263
BslFI GGGAC 3 cut(s) 627, 1708, 2147
BslI CCNNNNNNNGG 9 cut(s) 107, 163, 221, 504, 873, 1760, 2043, 2044, 2254
BsmAI GTCTC 4 cut(s) 181, 792, 1317, 2042
BsmBI CGTCTC 1 cut(s) 792
BsmFI GGGAC 3 cut(s) 627, 1708, 2147
BsmI GAATGC 1 cut(s) 868
BsnI GGCC 5 cut(s) 552, 1043, 1430, 1535, 2264
Bso31I GGTCTC 3 cut(s) 181, 1317, 2042
BsoBI CYCGRG 2 cut(s) 749, 1263
Bsp1286I GDGCHC 2 cut(s) 1543, 2037
Bsp13I TCCGGA 2 cut(s) 1276, 1679
Bsp19I CCATGG 3 cut(s) 948, 1754, 2253
BspACI CCGC 8 cut(s) 498, 538, 671, 791, 956, 1181, 1532, 2102
BspANI GGCC 5 cut(s) 552, 1043, 1430, 1535, 2264
BspCNI CTCAG 7 cut(s) 693, 961, 1323, 1804, 2122, 2142, 2196
BspEI TCCGGA 2 cut(s) 1276, 1679
BspLI GGNNCC 9 cut(s) 34, 297, 403, 615, 666, 1064, 1350, 1408, 2106
BspMI ACCTGC 1 cut(s) 393
BspPI GGATC 3 cut(s) 599, 681, 1116
BspT107I GGYRCC 7 cut(s) 32, 295, 401, 1062, 1348, 1406, 2104
BspTNI GGTCTC 3 cut(s) 181, 1317, 2042
BsrDI GCAATG 1 cut(s) 1242
BsrFI RCCGGY 3 cut(s) 1326, 1535, 2149
BssAI RCCGGY 3 cut(s) 1326, 1535, 2149
BssECI CCNNGG 4 cut(s) 867, 948, 1754, 2253
BssT1I CCWWGG 3 cut(s) 948, 1754, 2253
Bst2UI CCWGG 3 cut(s) 1045, 1249, 1631
Bst4CI ACNGT 3 cut(s) 115, 239, 772
Bst6I CTCTTC 4 cut(s) 90, 573, 1607, 2253
BstAPI GCANNNNNTGC 2 cut(s) 270, 1307
BstBAI YACGTR 2 cut(s) 292, 398
BstDEI CTNAG 8 cut(s) 701, 969, 1331, 1791, 1820, 2109, 2129, 2183
BstDSI CCRYGG 4 cut(s) 867, 948, 1754, 2253
BstEII GGTNACC 1 cut(s) 2152
BstF5I GGATG 9 cut(s) 676, 928, 1273, 1345, 1510, 1588, 1854, 1866, 2233
BstH2I RGCGCY 2 cut(s) 601, 2180
BstHHI GCGC 2 cut(s) 600, 2179
BstMAI GTCTC 4 cut(s) 181, 792, 1317, 2042
BstMCI CGRYCG 1 cut(s) 1536
BstMWI GCNNNNNNNGC 8 cut(s) 270, 494, 785, 1049, 1307, 1436, 1538, 1830
BstNI CCWGG 3 cut(s) 1045, 1249, 1631
BstPI GGTNACC 1 cut(s) 2152
BstSCI CCNGG 6 cut(s) 616, 1043, 1074, 1247, 1629, 2241
BstSFI CTRYAG 1 cut(s) 1081
BstV1I GCAGC 3 cut(s) 449, 800, 2166
BstV2I GAAGAC 2 cut(s) 572, 1755
BstX2I RGATCY 2 cut(s) 517, 1108
BstYI RGATCY 2 cut(s) 517, 1108
BstZI CGGCCG 1 cut(s) 1533
BsuRI GGCC 5 cut(s) 552, 1043, 1430, 1535, 2264
BtgI CCRYGG 4 cut(s) 867, 948, 1754, 2253
BtgZI GCGATG 3 cut(s) 706, 1454, 1827
BtrI CACGTC 2 cut(s) 198, 1675
BtsCI GGATG 9 cut(s) 676, 928, 1273, 1345, 1510, 1588, 1854, 1866, 2233
BtsIMutI CAGTG 3 cut(s) 1613, 1866, 2191
BveI ACCTGC 1 cut(s) 393
CfoI GCGC 2 cut(s) 600, 2179
Cfr10I RCCGGY 3 cut(s) 1326, 1535, 2149
Cfr13I GGNCC 6 cut(s) 95, 345, 614, 665, 1627, 2245
CseI GACGC 2 cut(s) 477, 1307
CsiI ACCWGGT 1 cut(s) 1629
Csp6I GTAC 5 cut(s) 116, 395, 1063, 1123, 1162
CspAI ACCGGT 1 cut(s) 2149
CviQI GTAC 5 cut(s) 116, 395, 1063, 1123, 1162
DdeI CTNAG 8 cut(s) 701, 969, 1331, 1791, 1820, 2109, 2129, 2183
DraIII CACNNNGTG 1 cut(s) 295
DriI GACNNNNNGTC 2 cut(s) 964, 2140
EaeI YGGCCR 2 cut(s) 1041, 1533
EagI CGGCCG 1 cut(s) 1533
Eam1104I CTCTTC 4 cut(s) 90, 573, 1607, 2253
Eam1105I GACNNNNNGTC 2 cut(s) 964, 2140
EarI CTCTTC 4 cut(s) 90, 573, 1607, 2253
EciI GGCGGA 2 cut(s) 686, 971
EclXI CGGCCG 1 cut(s) 1533
Eco130I CCWWGG 3 cut(s) 948, 1754, 2253
Eco24I GRGCYC 1 cut(s) 2037
Eco31I GGTCTC 3 cut(s) 181, 1317, 2042
Eco47I GGWCC 6 cut(s) 95, 345, 614, 665, 1627, 2245
Eco47III AGCGCT 1 cut(s) 599
Eco52I CGGCCG 1 cut(s) 1533
Eco57I CTGAAG 1 cut(s) 1589
Eco72I CACGTG 1 cut(s) 292
Eco88I CYCGRG 2 cut(s) 749, 1263
Eco91I GGTNACC 1 cut(s) 2152
EcoO109I RGGNCCY 1 cut(s) 1627
EcoO65I GGTNACC 1 cut(s) 2152
EcoRII CCWGG 3 cut(s) 1043, 1247, 1629
EcoT14I CCWWGG 3 cut(s) 948, 1754, 2253
EcoT22I ATGCAT 2 cut(s) 837, 1800
EcoT38I GRGCYC 1 cut(s) 2037
ErhI CCWWGG 3 cut(s) 948, 1754, 2253
Esp3I CGTCTC 1 cut(s) 792
FaqI GGGAC 3 cut(s) 627, 1708, 2147
FbaI TGATCA 1 cut(s) 1770
FblI GTMKAC 3 cut(s) 627, 1523, 2144
Fnu4HI GCNGC 6 cut(s) 438, 498, 789, 1182, 1533, 2180
FokI GGATG 9 cut(s) 663, 935, 1260, 1352, 1517, 1595, 1861, 1873, 2240
FriOI GRGCYC 1 cut(s) 2037
Fsp4HI GCNGC 6 cut(s) 438, 498, 789, 1182, 1533, 2180
FspBI CTAG 5 cut(s) 473, 545, 684, 822, 873
GlaI GCGC 2 cut(s) 599, 2178
GluI GCNGC 6 cut(s) 438, 498, 789, 1182, 1533, 2180
GsaI CCCAGC 2 cut(s) 1518, 2047
GsuI CTGGAG 1 cut(s) 84
HaeII RGCGCY 2 cut(s) 601, 2180
HaeIII GGCC 5 cut(s) 552, 1043, 1430, 1535, 2264
HgaI GACGC 2 cut(s) 477, 1307
HhaI GCGC 2 cut(s) 600, 2179
Hin6I GCGC 2 cut(s) 598, 2177
HinP1I GCGC 2 cut(s) 598, 2177
HincII GTYRAC 2 cut(s) 58, 201
HindII GTYRAC 2 cut(s) 58, 201
HindIII AAGCTT 2 cut(s) 647, 1445
HphI GGTGA 7 cut(s) 247, 1252, 1610, 2074, 2108, 2164, 2245
Hpy166II GTNNAC 6 cut(s) 58, 201, 395, 628, 1524, 2145
Hpy188III TCNNGA 8 cut(s) 101, 545, 562, 751, 1202, 1277, 1465, 1680
Hpy8I GTNNAC 6 cut(s) 58, 201, 395, 628, 1524, 2145
Hpy99I CGWCG 1 cut(s) 493
HpyCH4III ACNGT 3 cut(s) 115, 239, 772
HpyCH4IV ACGT 4 cut(s) 197, 291, 397, 1674
HpyCH4V TGCA 5 cut(s) 381, 835, 1093, 1659, 1798
HpyF10VI GCNNNNNNNGC 8 cut(s) 270, 494, 785, 1049, 1307, 1436, 1538, 1830
HpyF3I CTNAG 8 cut(s) 701, 969, 1331, 1791, 1820, 2109, 2129, 2183
HpySE526I ACGT 4 cut(s) 197, 291, 397, 1674
HspAI GCGC 2 cut(s) 598, 2177
Kpn2I TCCGGA 2 cut(s) 1276, 1679
KpnI GGTACC 1 cut(s) 1066
Ksp22I TGATCA 1 cut(s) 1770
LmnI GCTCC 5 cut(s) 136, 336, 1636, 1829, 1930
Lsp1109I GCAGC 3 cut(s) 449, 800, 2166
LweI GCATC 6 cut(s) 343, 822, 844, 1282, 1785, 2089
MabI ACCWGGT 1 cut(s) 1629
MaeI CTAG 5 cut(s) 473, 545, 684, 822, 873
MaeII ACGT 4 cut(s) 197, 291, 397, 1674
MaeIII GTNAC 8 cut(s) 358, 413, 639, 1070, 1670, 2152, 2160, 2233
MboII GAAGA 9 cut(s) 77, 80, 277, 577, 590, 1180, 1624, 1760, 2270
MflI RGATCY 2 cut(s) 517, 1108
MhlI GDGCHC 2 cut(s) 1543, 2037
MlsI TGGCCA 1 cut(s) 1043
MluNI TGGCCA 1 cut(s) 1043
MlyI GAGTC 8 cut(s) 881, 974, 1199, 1326, 1690, 2129, 2168, 2230
MmeI TCCRAC 3 cut(s) 372, 1347, 1716
Mox20I TGGCCA 1 cut(s) 1043
Mph1103I ATGCAT 2 cut(s) 837, 1800
MroI TCCGGA 2 cut(s) 1276, 1679
MroXI GAANNNNTTC 2 cut(s) 1256, 2213
MscI TGGCCA 1 cut(s) 1043
MseI TTAA 4 cut(s) 635, 659, 693, 1488
MslI CAYNNNNRTG 1 cut(s) 1664
Msp20I TGGCCA 1 cut(s) 1043
MspA1I CMGCKG 1 cut(s) 791
MspR9I CCNGG 6 cut(s) 618, 1045, 1076, 1249, 1631, 2243
Mva1269I GAATGC 1 cut(s) 868
MvaI CCWGG 3 cut(s) 1045, 1249, 1631
MwoI GCNNNNNNNGC 8 cut(s) 270, 494, 785, 1049, 1307, 1436, 1538, 1830
NciI CCSGG 3 cut(s) 618, 1076, 2243
NcoI CCATGG 3 cut(s) 948, 1754, 2253
NlaIV GGNNCC 9 cut(s) 34, 297, 403, 615, 666, 1064, 1350, 1408, 2106
NmuCI GTSAC 7 cut(s) 413, 639, 1070, 1670, 2152, 2160, 2233
NsiI ATGCAT 2 cut(s) 837, 1800
PaeR7I CTCGAG 1 cut(s) 749
PctI GAATGC 1 cut(s) 868
PdmI GAANNNNTTC 2 cut(s) 1256, 2213
PfeI GAWTC 7 cut(s) 529, 1029, 1086, 1268, 1804, 1894, 2125
PflFI GACNNNGTC 1 cut(s) 2158
PflMI CCANNNNNTGG 1 cut(s) 2254
PinAI ACCGGT 1 cut(s) 2149
PkrI GCNGC 6 cut(s) 439, 499, 790, 1183, 1534, 2181
PleI GAGTC 8 cut(s) 881, 973, 1199, 1325, 1690, 2129, 2167, 2230
PmaCI CACGTG 1 cut(s) 292
PmlI CACGTG 1 cut(s) 292
PpsI GAGTC 8 cut(s) 881, 973, 1199, 1325, 1690, 2129, 2167, 2230
Ppu21I YACGTR 2 cut(s) 292, 398
PpuMI RGGWCCY 1 cut(s) 1627
Psp5II RGGWCCY 1 cut(s) 1627
Psp6I CCWGG 3 cut(s) 1043, 1247, 1629
PspCI CACGTG 1 cut(s) 292
PspEI GGTNACC 1 cut(s) 2152
PspFI CCCAGC 2 cut(s) 1514, 2043
PspGI CCWGG 3 cut(s) 1043, 1247, 1629
PspN4I GGNNCC 9 cut(s) 34, 297, 403, 615, 666, 1064, 1350, 1408, 2106
PspPI GGNCC 6 cut(s) 95, 345, 614, 665, 1627, 2245
PspPPI RGGWCCY 1 cut(s) 1627
PsuI RGATCY 2 cut(s) 517, 1108
PsyI GACNNNGTC 1 cut(s) 2158
RsaI GTAC 5 cut(s) 117, 396, 1064, 1124, 1163
RsaNI GTAC 5 cut(s) 116, 395, 1063, 1123, 1162
RseI CAYNNNNRTG 1 cut(s) 1664
SaqAI TTAA 4 cut(s) 635, 659, 693, 1488
SatI GCNGC 6 cut(s) 438, 498, 789, 1182, 1533, 2180
Sau96I GGNCC 6 cut(s) 95, 345, 614, 665, 1627, 2245
ScaI AGTACT 1 cut(s) 1124
SchI GAGTC 8 cut(s) 881, 974, 1199, 1326, 1690, 2129, 2168, 2230
ScrFI CCNGG 6 cut(s) 618, 1045, 1076, 1249, 1631, 2243
SduI GDGCHC 2 cut(s) 1543, 2037
SexAI ACCWGGT 1 cut(s) 1629
SfaNI GCATC 6 cut(s) 343, 822, 844, 1282, 1785, 2089
SfcI CTRYAG 1 cut(s) 1081
Sfr274I CTCGAG 1 cut(s) 749
SgrAI CRCCGGYG 1 cut(s) 2149
SinI GGWCC 6 cut(s) 95, 345, 614, 665, 1627, 2245
SlaI CTCGAG 1 cut(s) 749
SmiMI CAYNNNNRTG 1 cut(s) 1664
SmlI CTYRAG 2 cut(s) 368, 749
SmoI CTYRAG 2 cut(s) 368, 749
SsiI CCGC 8 cut(s) 498, 538, 671, 791, 956, 1181, 1532, 2102
SspI AATATT 1 cut(s) 317
SspMI CTAG 5 cut(s) 473, 545, 684, 822, 873
StyD4I CCNGG 6 cut(s) 616, 1043, 1074, 1247, 1629, 2241
StyI CCWWGG 3 cut(s) 948, 1754, 2253
TaaI ACNGT 3 cut(s) 115, 239, 772
TaiI ACGT 4 cut(s) 200, 294, 400, 1677
TaqI TCGA 1 cut(s) 750
TaqII GACCGA 3 cut(s) 83, 245, 2171
TatI WGTACW 2 cut(s) 1122, 1161
TauI GCSGC 3 cut(s) 500, 1184, 1535
TfiI GAWTC 7 cut(s) 529, 1029, 1086, 1268, 1804, 1894, 2125
Tru1I TTAA 4 cut(s) 635, 659, 693, 1488
Tru9I TTAA 4 cut(s) 635, 659, 693, 1488
TscAI CASTG 3 cut(s) 1613, 1873, 2191
TseFI GTSAC 7 cut(s) 413, 639, 1070, 1670, 2152, 2160, 2233
TseI GCWGC 3 cut(s) 437, 788, 2179
Tsp45I GTSAC 7 cut(s) 413, 639, 1070, 1670, 2152, 2160, 2233
TspDTI ATGAA 8 cut(s) 17, 151, 160, 831, 1038, 1299, 1457, 1877
TspGWI ACGGA 1 cut(s) 977
TspRI CASTG 3 cut(s) 1613, 1873, 2191
Tth111I GACNNNGTC 1 cut(s) 2158
Van91I CCANNNNNTGG 1 cut(s) 2254
VpaK11BI GGWCC 6 cut(s) 95, 345, 614, 665, 1627, 2245
XapI RAATTY 4 cut(s) 915, 1144, 2200, 2287
XbaI TCTAGA 1 cut(s) 544
XcmI CCANNNNNNNNNTGG 1 cut(s) 865
XhoI CTCGAG 1 cut(s) 749
XmiI GTMKAC 3 cut(s) 627, 1523, 2144
XmnI GAANNNNTTC 2 cut(s) 1256, 2213
XspI CTAG 5 cut(s) 473, 545, 684, 822, 873
ZrmI AGTACT 1 cut(s) 1124
Zsp2I ATGCAT 2 cut(s) 837, 1800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.