MD14G1223900.v1.1

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000255
Physical Location & Seq
Reverse (-)
30491846 .. 30493822
1977 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1223900.v1.1.491

Sequence Viewer

Length: 1977 bp
ATGGCTCCTCCTTCTCCTCCACGTGCTATTCCTATTGTTGGTCCTCCACAGATCAACACCTTCGCCCCAGTAGCACAACCCAATCCCGCTCCCAGCGGGTCCCGACCGCCCATGGGCTTCACCGAGAACGGTTCGGGCACCTTCCTCTCCTCCGGCAACGCCTGCCTCGATCTCTTCTTCCAAATCGTACCATCCACCCCCGCCTCCTATATCAACCAACAACTTCCTCTGGCCTGGGCCCACGACGCCCTAACCACCCTCAAGCTCATATGCAACCTCCGTGGAGTCCGCGGCACCGGAAAGTCCGATAAGGAAGGGTTCTACACGGCGGCATTTTGGCTCCACAAGAACCACCCCAAAACCCTAGCCTGCAACCTCGCTTCCTTTGCCGAATTCGGGTATTTTAAAGACATGCCGGAGATTCTATACCGCCTTCTAGAAGGCCATGACGTCAGGAAGAAGCAAAGGGAGGAGTGGTCCATTGCAAAAGGAAGATTTCTCGGCCGTAGGAGACCGACAATCCGGCGACTGTTATGGAGAAGAACCCGACGTACTAGTACTAAAATCTCTGGAGCAATCAACGGCAGGAGACAGAGTGAGCCTAAAGCGGTTAAGATGATGAAGGCATTGGAGAGGGTAAAATTGGAAAAAGAAAAGGTGAGTGCTGCGAGGAAAGAGAAGAAGATCGCCATGGCGAAGAAGGCTGTTGCGAGGTACCAGCGTGACCCGGATTACCGGTTCTTGCACCATCAGGTTTCGGATCTCTTTGCGGAGTGCTTGAGGTCGGATCTCGAAAATTTCAAGTCCAATGAGTACAAGAAGATCACCCTGGCGGCGAAGTGGTGCCCTTCCCTCGACTCCTCCTTCGATCGCTCCACTCTTCTCTGTGAAGCGATTGCAAGGAAGGTGTTTCCGCGAGAATCCTACCTGGAATATGAAGGTGTAGAGGAGGCGCATTACGCGTACAGAGTGAGAGACCGGCTGAGGAAGGAGGTGCTGGTGCCGTTGAGGAAGGTGTTGCAGTTGCCCGAGGTGTATATGGGTGCTAATCAATGGGGAGCTATTCCGTACAATCGTGTGGCCTCCGTGGCCATGAAACTTTACAAAGAAAAGTTCATGAAGCACGATGAAGAGAGGTTTAAGAAGTATTTGGAGGACGTGAAGGCTGGAAAGTCTAAGATTGCGGCGGGTGCTCTGCTTCCTCATGAGATCATAGCCTCGCTGAAAGATGGTGATGGCGGGGAAGTGGGTGAGCTGCATTGGAAAAGAATGGTGGAGGACATGTTGAAACAAGGCAAGATGAAAAATTGTTTGGCTGTGTGTGATGTCTCGGGAAGCATGTGCGGGACTCCGATGGAGGTGTGTGTTGCTTTAGGACTGTTGTTGTCCGAACTGAGTGGAGAGCCGTGGAAGGGAAAGGTCATCACTTTCAGTCATGACCCTCAGCTACATTTGATACAAGGCGATGATCTAAGTTCGAAGTGCGAGTTTATAAGGAGGATGGAATGGGGTGCGAACACTGATTTCCAGAAAGTGTTTGATTTACTTTTGGAAGTGGCCGTGAATGGGAATTTGAAACCAGAGCATATGATCAAGAGAATCTTTGTGTTTAGCGACATGGAGTTCGATGAGGCTTCGGCTTCGAGTTGCAGGTATCCTGGGGGATGGGGTTGGGATACTGGTAGTGGTAGGGAGGAGAGCGGGAAGTGGGAGACTGATTATGAGGTAATACAAAGGAAGTTTGAGGAGAAAGGGTACGGGGATGTGGTGCCCCAGATTGTGTTTTGGAATCTGAGACACTCGCGTTCTACGCCAGTGTCTGGATCACAAAAAGGAGTGGCTCTCTTGAGTGGTTTCTCCAAGAATTTGATGAAATTGTTCTTGGATGAAGACGGTGAAATTGACATCAGCCCGGAACGTATTATGGAAGAGGCCATCTCCGGGGAATTGTACCAGAAGCTGGTTGTCATAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

659

Amino Acids

74.59

Weight (kDa)

9.1

Isoelectric Point (pI)

40.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2828 PF11443 41 - 434 1.3e-135 Domain of unknown function (DUF2828)
DUF7788 PF25043 436 - 644 2.1e-79 Domain of unknown function (DUF7788)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000255)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24780 AT5G13210 AT5G43390 AT5G43400
fragaria_vesca FvH4_4g23010 FvH4_5g12462 FvH4_5g12490 FvH4_5g12491 FvH4_5g20030 FvH4_5g20031 FvH4_5g21510 FvH4_6g30070
malus_domestica MD00G1095100.v1.1 MD05G1186800.v1.1 MD05G1259500.v1.1 MD05G1260200.v1.1 MD06G1159400.v1.1 MD06G1159500.v1.1 MD06G1162500.v1.1 MD06G1164600.v1.1 MD06G1187800.v1.1 MD06G1212600.v1.1 MD06G1212700.v1.1 MD13G1126800.v1.1 MD14G1168300.v1.1 MD14G1206600.v1.1 MD14G1223900.v1.1 MD14G1230800.v1.1 MD15G1240300.v1.1 MD15G1416800.v1.1 MD16G1127300.v1.1
prunus_persica Prupe.1G218700_v2.0.a1 Prupe.4G045100_v2.0.a1 Prupe.5G159300_v2.0.a1 Prupe.5G172200_v2.0.a1 Prupe.5G172300_v2.0.a1 Prupe.5G173200_v2.0.a1 Prupe.5G173300_v2.0.a1 Prupe.5G173400_v2.0.a1 Prupe.5G219000_v2.0.a1 Prupe.5G219100_v2.0.a1 Prupe.5G219200_v2.0.a1
pyrus_communis pycom05g24390 pycom06g19020 pycom10g15060 pycom10g24800 pycom13g11010 pycom16g10800 pycom16g10810 pycom17g22350 pycom17g22360
rosa_chinensis RchiOBHm_Chr2g0136111 RchiOBHm_Chr4g0429291 RchiOBHm_Chr7g0184981 RchiOBHm_Chr7g0184991 RchiOBHm_Chr7g0185001 RchiOBHm_Chr7g0204841
rosa_laevigata RLG00000003427 RLG00000004941 RLG00000004943 RLG00000004944 RLG00000004945 RLG00000004946 RLG00000007084 RLG00000013701 RLG00000019505 RLG00000019506
rosa_multiflora Rmu_co8294559.1_g000001 Rmu_co8375195.1_g000001 Rmu_co8450783.1_g000001 Rmu_co8519567.1_g000001 Rmu_sc0000054.1_g000008 Rmu_sc0001689.1_g000017 Rmu_sc0001801.1_g000027 Rmu_sc0002070.1_g000010 Rmu_sc0002180.1_g000022 Rmu_sc0002986.1_g000018 Rmu_sc0003221.1_g000027 Rmu_sc0005629.1_g000018 Rmu_sc0009898.1_g000002 Rmu_sc0018428.1_g000003 Rmu_sc0018428.1_g000005 Rmu_sc0022816.1_g000001 Rmu_sc0041781.1_g000001 Rmu_ssc0000289.1_g000007 Rmu_ssc0000289.1_g000008 Rmu_ssc0000289.1_g000010
rosa_roxburghii Rroxscaffold_2G00108460 Rroxscaffold_3G00252720 Rroxscaffold_3G00269290 Rroxscaffold_5G00349680 Rroxscaffold_5G00370900 Rroxscaffold_6G00415020
rosa_rugosa Rorug01G0043400 Rorug02G0328200 Rorug04G0234800 Rorug06G0465600 Rorug06G0465800 Rorug06G0465900 Rorug06G0466000 Rorug06G0466100 Rorug07G0086200 Rorug07G0086200
rosa_samantha Rh2BG386700 Rh2CG365900 Rh2DG402800 Rh4AG290400 Rh4BG296100 Rh4CG311900 Rh4DG293300 Rh7AG077000 Rh7AG077100 Rh7AG077200 Rh7AG216600 Rh7BG070200 Rh7BG070300 Rh7BG070400 Rh7BG213700 Rh7CG071400 Rh7CG071600 Rh7CG071700 Rh7CG071900 Rh7CG230100 Rh7DG070800 Rh7DG070900 Rh7DG224200 Rh7DG440100
rosa_wichuraiana Rw2G030970 Rw4G025150 Rw7G005840 Rw7G005850 Rw7G005860 Rw7G018770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1493
AatII GACGTC 1 cut(s) 453
Acc36I ACCTGC 1 cut(s) 1641
Acc65I GGTACC 1 cut(s) 714
AccB1I GGYRCC 6 cut(s) 137, 293, 714, 843, 1000, 1768
AccB7I CCANNNNNTGG 2 cut(s) 1820, 1960
AccBSI CCGCTC 2 cut(s) 89, 1701
AccII CGCG 4 cut(s) 291, 916, 962, 1804
AclWI GGATC 3 cut(s) 768, 795, 1831
AcoI YGGCCR 3 cut(s) 502, 1089, 1557
AcsI RAATTY 4 cut(s) 392, 796, 1570, 1864
AcvI CACGTG 1 cut(s) 23
AcyI GRCGYC 2 cut(s) 246, 450
AfaI GTAC 9 cut(s) 189, 553, 559, 716, 815, 965, 1070, 1757, 1952
AfiI CCNNNNNNNGG 8 cut(s) 38, 113, 396, 1412, 1566, 1820, 1941, 1960
AflIII ACRYGT 2 cut(s) 960, 1281
AgeI ACCGGT 1 cut(s) 735
AgsI TTSAA 3 cut(s) 802, 1288, 1576
AhlI ACTAGT 1 cut(s) 554
AjiI CACGTC 1 cut(s) 1159
AjnI CCWGG 4 cut(s) 233, 828, 927, 1657
AjuI GAANNNNNNNTTGG 4 cut(s) 1295, 1327, 1865, 1897
AloI GAACNNNNNNTCC 2 cut(s) 722, 754
AluBI AGCT 5 cut(s) 265, 1061, 1255, 1447, 1960
AluI AGCT 5 cut(s) 265, 1061, 1255, 1447, 1960
Alw21I GWGCWC 1 cut(s) 1195
Alw26I GTCTC 6 cut(s) 505, 583, 969, 1333, 1706, 1789
AlwI GGATC 3 cut(s) 768, 795, 1831
AlwNI CAGNNNCTG 2 cut(s) 1820, 1960
Ama87I CYCGRG 2 cut(s) 1028, 1330
AoxI GGCC 8 cut(s) 231, 237, 442, 502, 1080, 1089, 1557, 1932
ApaI GGGCCC 1 cut(s) 241
ApeKI GCWGC 2 cut(s) 665, 1255
ApoI RAATTY 4 cut(s) 392, 796, 1570, 1864
ArsI GACNNNNNNTTYG 4 cut(s) 848, 880, 1607, 1639
AsiGI ACCGGT 1 cut(s) 735
Asp700I GAANNNNTTC 1 cut(s) 1877
Asp718I GGTACC 1 cut(s) 714
AspLEI GCGC 1 cut(s) 955
AspS9I GGNCC 5 cut(s) 41, 99, 237, 238, 477
AsuC2I CCSGG 3 cut(s) 728, 1913, 1942
AsuHPI GGTGA 6 cut(s) 112, 670, 817, 1244, 1262, 1907
AsuII TTCGAA 1 cut(s) 1478
AvaI CYCGRG 2 cut(s) 1028, 1330
AvaII GGWCC 3 cut(s) 41, 99, 477
BaeGI GKGCMC 4 cut(s) 140, 241, 848, 1773
BaeI ACNNNNGTAYC 2 cut(s) 706, 739
BalI TGGCCA 1 cut(s) 1091
BanI GGYRCC 6 cut(s) 137, 293, 714, 843, 1000, 1768
BanII GRGCYC 1 cut(s) 241
BbrPI CACGTG 1 cut(s) 23
BbsI GAAGAC 1 cut(s) 1896
Bbv12I GWGCWC 1 cut(s) 1195
BbvCI CCTCAGC 2 cut(s) 983, 1443
BbvI GCAGC 2 cut(s) 652, 1242
BccI CCATC 8 cut(s) 199, 756, 1223, 1229, 1348, 1495, 1659, 1943
BceAI ACGGC 6 cut(s) 342, 489, 598, 988, 1390, 1544
BciT130I CCWGG 4 cut(s) 235, 830, 929, 1659
BciVI GTATCC 2 cut(s) 1665, 1669
BclI TGATCA 1 cut(s) 1590
BcnI CCSGG 3 cut(s) 728, 1913, 1942
BcoDI GTCTC 6 cut(s) 505, 583, 969, 1333, 1706, 1789
BcuI ACTAGT 1 cut(s) 554
BfaI CTAG 3 cut(s) 365, 437, 555
BfuAI ACCTGC 1 cut(s) 1641
BfuI GTATCC 2 cut(s) 1665, 1669
BglI GCCNNNNNGGC 1 cut(s) 1088
BisI GCNGC 6 cut(s) 292, 330, 666, 834, 1185, 1256
BlsI GCNGC 6 cut(s) 293, 331, 667, 835, 1186, 1257
BmcAI AGTACT 1 cut(s) 559
Bme1390I CCNGG 7 cut(s) 235, 728, 830, 929, 1659, 1913, 1942
Bme18I GGWCC 3 cut(s) 41, 99, 477
BmeT110I CYCGRG 2 cut(s) 1028, 1330
BmgBI CACGTC 1 cut(s) 1159
BmgT120I GGNCC 5 cut(s) 41, 99, 237, 238, 477
BmrFI CCNGG 7 cut(s) 235, 728, 830, 929, 1659, 1913, 1942
BmrI ACTGGG 1 cut(s) 62
BmuI ACTGGG 1 cut(s) 62
BpiI GAAGAC 1 cut(s) 1896
BplI GAGNNNNNCTC 4 cut(s) 1827, 1859, 1922, 1954
BpmI CTGGAG 1 cut(s) 591
Bpu10I CCTNAGC 2 cut(s) 983, 1443
Bpu14I TTCGAA 1 cut(s) 1478
BpuEI CTTGAG 3 cut(s) 245, 799, 1867
BpuMI CCSGG 3 cut(s) 728, 1913, 1942
BsaAI YACGTR 1 cut(s) 23
BsaHI GRCGYC 2 cut(s) 246, 450
BsaI GGTCTC 2 cut(s) 505, 969
BsaWI WCCGGW 2 cut(s) 296, 735
BsaXI ACNNNNNCTCC 8 cut(s) 461, 491, 580, 610, 1268, 1298, 1739, 1769
Bsc4I CCNNNNNNNGG 8 cut(s) 38, 113, 396, 1412, 1566, 1820, 1941, 1960
Bse118I RCCGGY 2 cut(s) 735, 978
Bse1I ACTGG 3 cut(s) 68, 1684, 1814
Bse3DI GCAATG 1 cut(s) 480
BseBI CCWGG 4 cut(s) 235, 830, 929, 1659
BseGI GGATG 5 cut(s) 191, 1506, 1670, 1768, 1891
BseLI CCNNNNNNNGG 8 cut(s) 38, 113, 396, 1412, 1566, 1820, 1941, 1960
BseMI GCAATG 1 cut(s) 480
BseMII CTCAG 4 cut(s) 974, 1385, 1457, 1784
BseNI ACTGG 3 cut(s) 68, 1684, 1814
BseRI GAGGAG 7 cut(s) 6, 139, 485, 850, 962, 1709, 1760
BseSI GKGCMC 4 cut(s) 140, 241, 848, 1773
BseX3I CGGCCG 1 cut(s) 502
BseXI GCAGC 2 cut(s) 652, 1242
BseYI CCCAGC 1 cut(s) 92
Bsh1236I CGCG 4 cut(s) 291, 916, 962, 1804
Bsh1285I CGRYCG 3 cut(s) 107, 505, 871
BshFI GGCC 8 cut(s) 233, 239, 444, 504, 1082, 1091, 1559, 1934
BshNI GGYRCC 6 cut(s) 137, 293, 714, 843, 1000, 1768
BshTI ACCGGT 1 cut(s) 735
BsiEI CGRYCG 3 cut(s) 107, 505, 871
BsiHKAI GWGCWC 1 cut(s) 1195
BsiHKCI CYCGRG 2 cut(s) 1028, 1330
BsiSI CCGG 9 cut(s) 153, 297, 416, 523, 728, 736, 979, 1913, 1941
BslFI GGGAC 2 cut(s) 85, 1360
BslI CCNNNNNNNGG 8 cut(s) 38, 113, 396, 1412, 1566, 1820, 1941, 1960
BsmAI GTCTC 6 cut(s) 505, 583, 969, 1333, 1706, 1789
BsmFI GGGAC 2 cut(s) 85, 1360
BsnI GGCC 8 cut(s) 233, 239, 444, 504, 1082, 1091, 1559, 1934
Bso31I GGTCTC 2 cut(s) 505, 969
BsoBI CYCGRG 2 cut(s) 1028, 1330
Bsp119I TTCGAA 1 cut(s) 1478
Bsp120I GGGCCC 1 cut(s) 237
Bsp1286I GDGCHC 5 cut(s) 140, 241, 848, 1195, 1773
Bsp19I CCATGG 2 cut(s) 111, 690
BspANI GGCC 8 cut(s) 233, 239, 444, 504, 1082, 1091, 1559, 1934
BspCNI CTCAG 4 cut(s) 975, 1386, 1456, 1785
BspFNI CGCG 4 cut(s) 291, 916, 962, 1804
BspHI TCATGA 3 cut(s) 1116, 1204, 1435
BspMI ACCTGC 1 cut(s) 1641
BspPI GGATC 3 cut(s) 768, 795, 1831
BspT104I TTCGAA 1 cut(s) 1478
BspT107I GGYRCC 6 cut(s) 137, 293, 714, 843, 1000, 1768
BspTNI GGTCTC 2 cut(s) 505, 969
BsrBI CCGCTC 2 cut(s) 89, 1701
BsrDI GCAATG 1 cut(s) 480
BsrFI RCCGGY 2 cut(s) 735, 978
BsrI ACTGG 3 cut(s) 68, 1684, 1814
BssAI RCCGGY 2 cut(s) 735, 978
BssNI GRCGYC 2 cut(s) 246, 450
BssT1I CCWWGG 2 cut(s) 111, 690
Bst2UI CCWGG 4 cut(s) 235, 830, 929, 1659
Bst4CI ACNGT 4 cut(s) 131, 531, 1380, 1895
Bst6I CTCTTC 4 cut(s) 179, 885, 1125, 1923
BstACI GRCGYC 2 cut(s) 246, 450
BstAPI GCANNNNNTGC 1 cut(s) 162
BstBAI YACGTR 1 cut(s) 23
BstBI TTCGAA 1 cut(s) 1478
BstC8I GCNNGC 2 cut(s) 163, 370
BstDEI CTNAG 6 cut(s) 983, 1176, 1394, 1443, 1472, 1793
BstDSI CCRYGG 6 cut(s) 111, 280, 289, 690, 1086, 1406
BstF5I GGATG 5 cut(s) 191, 1506, 1670, 1768, 1891
BstFNI CGCG 4 cut(s) 291, 916, 962, 1804
BstHHI GCGC 1 cut(s) 955
BstMAI GTCTC 6 cut(s) 505, 583, 969, 1333, 1706, 1789
BstMCI CGRYCG 3 cut(s) 107, 505, 871
BstMWI GCNNNNNNNGC 9 cut(s) 71, 162, 245, 386, 701, 959, 1088, 1190, 1810
BstNI CCWGG 4 cut(s) 235, 830, 929, 1659
BstNSI RCATGY 3 cut(s) 415, 1285, 1342
BstSCI CCNGG 7 cut(s) 233, 726, 828, 927, 1657, 1911, 1940
BstSLI GKGCMC 4 cut(s) 140, 241, 848, 1773
BstUI CGCG 4 cut(s) 291, 916, 962, 1804
BstV1I GCAGC 2 cut(s) 652, 1242
BstV2I GAAGAC 1 cut(s) 1896
BstX2I RGATCY 2 cut(s) 760, 787
BstYI RGATCY 2 cut(s) 760, 787
BstZI CGGCCG 1 cut(s) 502
BsuI GTATCC 2 cut(s) 1665, 1669
BsuRI GGCC 8 cut(s) 233, 239, 444, 504, 1082, 1091, 1559, 1934
BtgI CCRYGG 6 cut(s) 111, 280, 289, 690, 1086, 1406
BtgZI GCGATG 1 cut(s) 1479
BtrI CACGTC 1 cut(s) 1159
BtsCI GGATG 5 cut(s) 191, 1506, 1670, 1768, 1891
BtsIMutI CAGTG 2 cut(s) 1518, 1821
BveI ACCTGC 1 cut(s) 1641
Cac8I GCNNGC 2 cut(s) 163, 370
CaiI CAGNNNCTG 2 cut(s) 1820, 1960
CciI TCATGA 3 cut(s) 1116, 1204, 1435
CfoI GCGC 1 cut(s) 955
Cfr10I RCCGGY 2 cut(s) 735, 978
Cfr13I GGNCC 5 cut(s) 41, 99, 237, 238, 477
Cfr42I CCGCGG 1 cut(s) 292
CseI GACGC 1 cut(s) 254
Csp6I GTAC 9 cut(s) 188, 552, 558, 715, 814, 964, 1069, 1756, 1951
CspAI ACCGGT 1 cut(s) 735
CspCI CAANNNNNGTGG 2 cut(s) 262, 297
CviQI GTAC 9 cut(s) 188, 552, 558, 715, 814, 964, 1069, 1756, 1951
DdeI CTNAG 6 cut(s) 983, 1176, 1394, 1443, 1472, 1793
DraI TTTAAA 1 cut(s) 406
EaeI YGGCCR 3 cut(s) 502, 1089, 1557
EagI CGGCCG 1 cut(s) 502
Eam1104I CTCTTC 4 cut(s) 179, 885, 1125, 1923
EarI CTCTTC 4 cut(s) 179, 885, 1125, 1923
EclXI CGGCCG 1 cut(s) 502
Eco130I CCWWGG 2 cut(s) 111, 690
Eco24I GRGCYC 1 cut(s) 241
Eco31I GGTCTC 2 cut(s) 505, 969
Eco47I GGWCC 3 cut(s) 41, 99, 477
Eco52I CGGCCG 1 cut(s) 502
Eco72I CACGTG 1 cut(s) 23
Eco88I CYCGRG 2 cut(s) 1028, 1330
EcoO109I RGGNCCY 1 cut(s) 99
EcoRI GAATTC 1 cut(s) 392
EcoRII CCWGG 4 cut(s) 233, 828, 927, 1657
EcoT14I CCWWGG 2 cut(s) 111, 690
EcoT38I GRGCYC 1 cut(s) 241
ErhI CCWWGG 2 cut(s) 111, 690
FalI AAGNNNNNCTT 2 cut(s) 1586, 1618
FaqI GGGAC 2 cut(s) 85, 1360
FauI CCCGC 7 cut(s) 89, 94, 208, 1180, 1232, 1337, 1694
FauNDI CATATG 2 cut(s) 269, 1587
FbaI TGATCA 1 cut(s) 1590
Fnu4HI GCNGC 6 cut(s) 292, 330, 666, 834, 1185, 1256
FokI GGATG 5 cut(s) 178, 1513, 1677, 1775, 1898
FriOI GRGCYC 1 cut(s) 241
Fsp4HI GCNGC 6 cut(s) 292, 330, 666, 834, 1185, 1256
FspBI CTAG 3 cut(s) 365, 437, 555
GlaI GCGC 1 cut(s) 954
GluI GCNGC 6 cut(s) 292, 330, 666, 834, 1185, 1256
GsaI CCCAGC 1 cut(s) 96
GsuI CTGGAG 1 cut(s) 591
HaeIII GGCC 8 cut(s) 233, 239, 444, 504, 1082, 1091, 1559, 1934
HapII CCGG 9 cut(s) 153, 297, 416, 523, 728, 736, 979, 1913, 1941
HgaI GACGC 1 cut(s) 254
HhaI GCGC 1 cut(s) 955
Hin1I GRCGYC 2 cut(s) 246, 450
Hin6I GCGC 1 cut(s) 953
HinP1I GCGC 1 cut(s) 953
HinfI GANTC 7 cut(s) 285, 421, 857, 920, 1348, 1599, 1789
HpaII CCGG 9 cut(s) 153, 297, 416, 523, 728, 736, 979, 1913, 1941
HphI GGTGA 6 cut(s) 112, 670, 817, 1244, 1262, 1907
Hpy188I TCNGA 6 cut(s) 307, 760, 787, 1353, 1390, 1794
Hpy99I CGWCG 2 cut(s) 248, 552
HpyCH4III ACNGT 4 cut(s) 131, 531, 1380, 1895
HpyCH4IV ACGT 5 cut(s) 22, 450, 550, 1158, 1918
HpyCH4V TGCA 8 cut(s) 273, 372, 485, 745, 899, 1021, 1258, 1650
HpyF10VI GCNNNNNNNGC 9 cut(s) 71, 162, 245, 386, 701, 959, 1088, 1190, 1810
HpyF3I CTNAG 6 cut(s) 983, 1176, 1394, 1443, 1472, 1793
HpySE526I ACGT 5 cut(s) 22, 450, 550, 1158, 1918
Hsp92I GRCGYC 2 cut(s) 246, 450
HspAI GCGC 1 cut(s) 953
KflI GGGWCCC 1 cut(s) 99
KpnI GGTACC 1 cut(s) 718
Ksp22I TGATCA 1 cut(s) 1590
KspI CCGCGG 1 cut(s) 292
LmnI GCTCC 6 cut(s) 10, 94, 345, 572, 878, 1058
Lsp1109I GCAGC 2 cut(s) 652, 1242
MaeI CTAG 3 cut(s) 365, 437, 555
MaeII ACGT 5 cut(s) 22, 450, 550, 1158, 1918
MaeIII GTNAC 1 cut(s) 722
MbiI CCGCTC 2 cut(s) 89, 1701
MflI RGATCY 2 cut(s) 760, 787
MhlI GDGCHC 5 cut(s) 140, 241, 848, 1195, 1773
MlsI TGGCCA 1 cut(s) 1091
MluCI AATT 9 cut(s) 392, 641, 796, 1306, 1570, 1864, 1874, 1899, 1946
MluI ACGCGT 1 cut(s) 960
MluNI TGGCCA 1 cut(s) 1091
MlyI GAGTC 3 cut(s) 294, 851, 1342
MmeI TCCRAC 1 cut(s) 765
Mox20I TGGCCA 1 cut(s) 1091
MroXI GAANNNNTTC 1 cut(s) 1877
MscI TGGCCA 1 cut(s) 1091
MseI TTAA 3 cut(s) 405, 612, 1140
Msp20I TGGCCA 1 cut(s) 1091
MspA1I CMGCKG 2 cut(s) 96, 291
MspI CCGG 9 cut(s) 153, 297, 416, 523, 728, 736, 979, 1913, 1941
MspR9I CCNGG 7 cut(s) 235, 728, 830, 929, 1659, 1913, 1942
MvaI CCWGG 4 cut(s) 235, 830, 929, 1659
MvnI CGCG 4 cut(s) 291, 916, 962, 1804
MwoI GCNNNNNNNGC 9 cut(s) 71, 162, 245, 386, 701, 959, 1088, 1190, 1810
NciI CCSGG 3 cut(s) 728, 1913, 1942
NcoI CCATGG 2 cut(s) 111, 690
NdeI CATATG 2 cut(s) 269, 1587
NmeAIII GCCGAG 1 cut(s) 480
NmuCI GTSAC 1 cut(s) 722
NspI RCATGY 3 cut(s) 415, 1285, 1342
NspV TTCGAA 1 cut(s) 1478
PagI TCATGA 3 cut(s) 1116, 1204, 1435
PciI ACATGT 1 cut(s) 1281
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PdmI GAANNNNTTC 1 cut(s) 1877
PfeI GAWTC 4 cut(s) 421, 920, 1599, 1789
PflMI CCANNNNNTGG 2 cut(s) 1820, 1960
PinAI ACCGGT 1 cut(s) 735
PkrI GCNGC 6 cut(s) 293, 331, 667, 835, 1186, 1257
Ple19I CGATCG 1 cut(s) 871
PleI GAGTC 3 cut(s) 293, 851, 1342
PmaCI CACGTG 1 cut(s) 23
PmlI CACGTG 1 cut(s) 23
PpsI GAGTC 3 cut(s) 293, 851, 1342
Ppu21I YACGTR 1 cut(s) 23
PpuMI RGGWCCY 1 cut(s) 99
PscI ACATGT 1 cut(s) 1281
PsiI TTATAA 1 cut(s) 1493
Psp5II RGGWCCY 1 cut(s) 99
Psp6I CCWGG 4 cut(s) 233, 828, 927, 1657
PspCI CACGTG 1 cut(s) 23
PspFI CCCAGC 1 cut(s) 92
PspGI CCWGG 4 cut(s) 233, 828, 927, 1657
PspOMI GGGCCC 1 cut(s) 237
PspPI GGNCC 5 cut(s) 41, 99, 237, 238, 477
PspPPI RGGWCCY 1 cut(s) 99
PsrI GAACNNNNNNTAC 2 cut(s) 535, 567
PstNI CAGNNNCTG 2 cut(s) 1820, 1960
PsuI RGATCY 2 cut(s) 760, 787
PvuI CGATCG 1 cut(s) 871
RsaI GTAC 9 cut(s) 189, 553, 559, 716, 815, 965, 1070, 1757, 1952
RsaNI GTAC 9 cut(s) 188, 552, 558, 715, 814, 964, 1069, 1756, 1951
SacII CCGCGG 1 cut(s) 292
SaqAI TTAA 3 cut(s) 405, 612, 1140
SatI GCNGC 6 cut(s) 292, 330, 666, 834, 1185, 1256
Sau96I GGNCC 5 cut(s) 41, 99, 237, 238, 477
ScaI AGTACT 1 cut(s) 559
SchI GAGTC 3 cut(s) 294, 851, 1342
ScrFI CCNGG 7 cut(s) 235, 728, 830, 929, 1659, 1913, 1942
SduI GDGCHC 5 cut(s) 140, 241, 848, 1195, 1773
SfiI GGCCNNNNNGGCC 1 cut(s) 1088
Sfr303I CCGCGG 1 cut(s) 292
SfuI TTCGAA 1 cut(s) 1478
SgrBI CCGCGG 1 cut(s) 292
SinI GGWCC 3 cut(s) 41, 99, 477
SmlI CTYRAG 3 cut(s) 260, 778, 1846
SmoI CTYRAG 3 cut(s) 260, 778, 1846
SpeI ACTAGT 1 cut(s) 554
Sse9I AATT 9 cut(s) 392, 641, 796, 1306, 1570, 1864, 1874, 1899, 1946
SspMI CTAG 3 cut(s) 365, 437, 555
StyD4I CCNGG 7 cut(s) 233, 726, 828, 927, 1657, 1911, 1940
StyI CCWWGG 2 cut(s) 111, 690
TaaI ACNGT 4 cut(s) 131, 531, 1380, 1895
TaiI ACGT 5 cut(s) 25, 453, 553, 1161, 1921
TaqI TCGA 7 cut(s) 168, 792, 855, 867, 1478, 1626, 1643
TaqII GACCGA 1 cut(s) 529
TasI AATT 9 cut(s) 392, 641, 796, 1306, 1570, 1864, 1874, 1899, 1946
TatI WGTACW 2 cut(s) 557, 813
TauI GCSGC 4 cut(s) 294, 332, 836, 1187
TfiI GAWTC 4 cut(s) 421, 920, 1599, 1789
Tru1I TTAA 3 cut(s) 405, 612, 1140
Tru9I TTAA 3 cut(s) 405, 612, 1140
TscAI CASTG 2 cut(s) 1525, 1821
TseFI GTSAC 1 cut(s) 722
TseI GCWGC 2 cut(s) 665, 1255
Tsp45I GTSAC 1 cut(s) 722
TspDTI ATGAA 9 cut(s) 635, 951, 1105, 1109, 1133, 1143, 1316, 1886, 1902
TspGWI ACGGA 3 cut(s) 269, 1056, 1075
TspRI CASTG 2 cut(s) 1525, 1821
Van91I CCANNNNNTGG 2 cut(s) 1820, 1960
VpaK11BI GGWCC 3 cut(s) 41, 99, 477
XapI RAATTY 4 cut(s) 392, 796, 1570, 1864
XbaI TCTAGA 1 cut(s) 436
XceI RCATGY 3 cut(s) 415, 1285, 1342
XmnI GAANNNNTTC 1 cut(s) 1877
XspI CTAG 3 cut(s) 365, 437, 555
ZraI GACGTC 1 cut(s) 451
ZrmI AGTACT 1 cut(s) 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.