Rroxscaffold_3G00252720

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000255
Physical Location & Seq
Forward (+)
46780676 .. 46783139
2464 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00252720.1

Sequence Viewer

Length: 1977 bp
ATGCTTCCTTATACTACTATAAGAACTGCATTGAGACCCAAATCATCAGACTCCACCACCACCGCCACAACGAAACCTCCGTTGGGACGCACCGAGAACAACTCCGCCACCTACCTCTCCTCCGGCAACCCCTGCCTCGACCTCTTCTTCCGCATCGTACCGGACACCCCGTCCTCTTACATCAACGAACAACTTCCCGAGTCCTGGGCCCACAACGCCCTAACCACCCTCAAGCTAATCTGCAACCTCCGCGGCGTCCGCGGCACTGGAAAGTCAGACAAAGAAGCCTTCTTCACGGCAGCATTTTGGCTCCACAGACACCACCCCAAAACCCTAGCCTGCAATCTCGCCTCTTTCGCCCAATTCGGGTGCTTCAAAGACCTCCCCGAGATTCTCTACCGGCTTCTAGAAGGCCAAGACATAAGGAAGAACCGCAAGGGCGAGTGGTCCATGAAAAAGAAACTCTCCCGCCGCCGTAGAGGTTCAAAATCTCCGTCACGCTCAAGTTCTAGCTCAAGTTCTAGGTCATACTCAAGAGATAGAGAGAGCAACTGTACGTCTCGATCAAGAACACGATCTCGGTCGCCCCCGCAGCGGCGGAGGCCAGGTTTTAAGTCGAGAACCAACAAAAGGAAGAGGCTGAGTTACACCACCCGGAAGGAGCCGAGGGAGGAGCGGGTGGTGAAGGCGACGGAGAGGACAAACTCGGAAAAGGAAAAGGCAAGTGTTTTGAGAAAACAGAAATTCACTGACATGGCAAACAAGGCCGTCTCGAGATACGATCGCGACCCGGATTACCGGTTCCTCCACGAGCGCGTCTCGGATCTCTTTGCCGCTTGCTTGAAATCCGACCTCGAAAATCTCAACTCTAATAAGCTCAACAACATCAGCCTCGCCGCCAAGTGGTGCCCTTCGCTCGACTCGTCTTTTGATCGCGCCACTCTCCTCTGCGAAAGGATTGCAAAGAAGGTTTTCCCGATCGAGTCGTGTCCGGAATACGAGGGCGTTTTGGAGCAGCATTATGCTTACAGAGTCCGGGACCGGCTGAGGAAGGATGTGCTTGTTCCTTTAAGGAAGGCTCTGGAGTTACCGGAGGTTTATATGGGGGAACGAAAATGGAGGTCCCTTCCTTACAATAGGGTCCCCTCCGTGGCCATGAAGCTTTACAAGGAAACGTTTTTTAAGCACGACCTTGTGAGGTTTTCGAAGTATTTGGTTGATGCAAAGGCCGGGAAGACCACCATTGCGGCCGGTGCTCTGCTGCCGCACGAGATCATTGCCTCGAATTCAAGAGGCAGTGATGGCGTGGCGGAGCTTCAGTGGAAGAGGATGGTGGAGGATTTGGTGAAGAAGGGGCAGCTGAAGAACTGCTTGGCTGTGTGTGACGTGTCAGGCAGCATGGAGGGGCTTCCTATGGATGTGAGCGTGGCCTTGGGGCTTTTGGTTTCGGAATTAAGCGAAAAGCCGTGGAAAGGGAAGGTCATCACTTTTAGTGAGAAGCCTGAGCTGCATTTGATTGAAGGTGAAGATTTGAAGTCAAAGTGTACTTTTATGAGGTCAATGGACTCGGGGTTCAACACCGATTTTCAAAAGGTGTTTGATCTGATACTTAGAGTGGCTGTGAAGGGGAACTTAAAGCCTGAGGATATGATAAAGAGGGTGTTTGTGTTTAGTGACATGGAGTTTGATGAGGCTAATCATGAGAGTACTTGGGAGACGGATTATGAGGCGATTCAGAGGAGGTTCAAGAGGAATGGTTATGGGAATGTGGTGCCTCAGATTGTGTTTTGGAATCTGAGGCACTCTATGTCTACGCCTGTGACGGAGAAGCAACCTGGGGTGGCAATGTTGAGTGGCTTTTCAAAGAATTTGTTGAAGTTGTTCTTGGATAATGATGGGGAAGTTAGCCCGGATATGGCCATGGAGTTGGCCATCTCCGGCGATGAGTATCAGAAACTGGCTGTGGTCGACAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

658

Amino Acids

75.23

Weight (kDa)

9.63

Isoelectric Point (pI)

50.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2828 PF11443 31 - 454 2.2e-132 Domain of unknown function (DUF2828)
DUF7788 PF25043 456 - 636 1.3e-79 Domain of unknown function (DUF7788)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000255)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24780 AT5G13210 AT5G43390 AT5G43400
fragaria_vesca FvH4_4g23010 FvH4_5g12462 FvH4_5g12490 FvH4_5g12491 FvH4_5g20030 FvH4_5g20031 FvH4_5g21510 FvH4_6g30070
malus_domestica MD00G1095100.v1.1 MD05G1186800.v1.1 MD05G1259500.v1.1 MD05G1260200.v1.1 MD06G1159400.v1.1 MD06G1159500.v1.1 MD06G1162500.v1.1 MD06G1164600.v1.1 MD06G1187800.v1.1 MD06G1212600.v1.1 MD06G1212700.v1.1 MD13G1126800.v1.1 MD14G1168300.v1.1 MD14G1206600.v1.1 MD14G1223900.v1.1 MD14G1230800.v1.1 MD15G1240300.v1.1 MD15G1416800.v1.1 MD16G1127300.v1.1
prunus_persica Prupe.1G218700_v2.0.a1 Prupe.4G045100_v2.0.a1 Prupe.5G159300_v2.0.a1 Prupe.5G172200_v2.0.a1 Prupe.5G172300_v2.0.a1 Prupe.5G173200_v2.0.a1 Prupe.5G173300_v2.0.a1 Prupe.5G173400_v2.0.a1 Prupe.5G219000_v2.0.a1 Prupe.5G219100_v2.0.a1 Prupe.5G219200_v2.0.a1
pyrus_communis pycom05g24390 pycom06g19020 pycom10g15060 pycom10g24800 pycom13g11010 pycom16g10800 pycom16g10810 pycom17g22350 pycom17g22360
rosa_chinensis RchiOBHm_Chr2g0136111 RchiOBHm_Chr4g0429291 RchiOBHm_Chr7g0184981 RchiOBHm_Chr7g0184991 RchiOBHm_Chr7g0185001 RchiOBHm_Chr7g0204841
rosa_laevigata RLG00000003427 RLG00000004941 RLG00000004943 RLG00000004944 RLG00000004945 RLG00000004946 RLG00000007084 RLG00000013701 RLG00000019505 RLG00000019506
rosa_multiflora Rmu_co8294559.1_g000001 Rmu_co8375195.1_g000001 Rmu_co8450783.1_g000001 Rmu_co8519567.1_g000001 Rmu_sc0000054.1_g000008 Rmu_sc0001689.1_g000017 Rmu_sc0001801.1_g000027 Rmu_sc0002070.1_g000010 Rmu_sc0002180.1_g000022 Rmu_sc0002986.1_g000018 Rmu_sc0003221.1_g000027 Rmu_sc0005629.1_g000018 Rmu_sc0009898.1_g000002 Rmu_sc0018428.1_g000003 Rmu_sc0018428.1_g000005 Rmu_sc0022816.1_g000001 Rmu_sc0041781.1_g000001 Rmu_ssc0000289.1_g000007 Rmu_ssc0000289.1_g000008 Rmu_ssc0000289.1_g000010
rosa_roxburghii Rroxscaffold_2G00108460 Rroxscaffold_3G00252720 Rroxscaffold_3G00269290 Rroxscaffold_5G00349680 Rroxscaffold_5G00370900 Rroxscaffold_6G00415020
rosa_rugosa Rorug01G0043400 Rorug02G0328200 Rorug04G0234800 Rorug06G0465600 Rorug06G0465800 Rorug06G0465900 Rorug06G0466000 Rorug06G0466100 Rorug07G0086200 Rorug07G0086200
rosa_samantha Rh2BG386700 Rh2CG365900 Rh2DG402800 Rh4AG290400 Rh4BG296100 Rh4CG311900 Rh4DG293300 Rh7AG077000 Rh7AG077100 Rh7AG077200 Rh7AG216600 Rh7BG070200 Rh7BG070300 Rh7BG070400 Rh7BG213700 Rh7CG071400 Rh7CG071600 Rh7CG071700 Rh7CG071900 Rh7CG230100 Rh7DG070800 Rh7DG070900 Rh7DG224200 Rh7DG440100
rosa_wichuraiana Rw2G030970 Rw4G025150 Rw7G005840 Rw7G005850 Rw7G005860 Rw7G018770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 906, 1771
AccBSI CCGCTC 1 cut(s) 676
AccI GTMKAC 2 cut(s) 1811, 1968
AccII CGCG 5 cut(s) 252, 261, 786, 816, 936
AccIII TCCGGA 1 cut(s) 991
AclI AACGTT 1 cut(s) 1175
AclWI GGATC 1 cut(s) 831
AcoI YGGCCR 4 cut(s) 1152, 1248, 1917, 1929
AcsI RAATTY 3 cut(s) 743, 1285, 1867
AcuI CTGAAG 2 cut(s) 1301, 1382
AcyI GRCGYC 1 cut(s) 255
AfaI GTAC 4 cut(s) 159, 556, 1546, 1708
AfiI CCNNNNNNNGG 9 cut(s) 83, 204, 366, 594, 630, 903, 1150, 1472, 1915
AflIII ACRYGT 1 cut(s) 1386
AgeI ACCGGT 1 cut(s) 798
AhdI GACNNNNNGTC 1 cut(s) 169
AjiI CACGTC 1 cut(s) 1387
AjnI CCWGG 3 cut(s) 203, 604, 1834
AjuI GAANNNNNNNTTGG 6 cut(s) 65, 97, 1355, 1387, 1868, 1900
AloI GAACNNNNNNTCC 4 cut(s) 785, 817, 1556, 1588
AluBI AGCT 7 cut(s) 235, 513, 877, 1162, 1315, 1360, 1507
AluI AGCT 7 cut(s) 235, 513, 877, 1162, 1315, 1360, 1507
Alw21I GWGCWC 1 cut(s) 1258
Alw26I GTCTC 5 cut(s) 28, 564, 775, 823, 1709
AlwI GGATC 1 cut(s) 831
AlwNI CAGNNNCTG 1 cut(s) 1957
Ama87I CYCGRG 4 cut(s) 197, 386, 772, 1567
Aor13HI TCCGGA 1 cut(s) 991
ApaI GGGCCC 1 cut(s) 211
ApeKI GCWGC 7 cut(s) 299, 592, 1015, 1261, 1357, 1395, 1507
ApoI RAATTY 3 cut(s) 743, 1285, 1867
ArsI GACNNNNNNTTYG 6 cut(s) 479, 511, 911, 943, 1667, 1699
AsiGI ACCGGT 1 cut(s) 798
Asp700I GAANNNNTTC 3 cut(s) 192, 971, 1880
AspLEI GCGC 2 cut(s) 816, 938
AspS9I GGNCC 6 cut(s) 207, 208, 447, 1039, 1122, 1141
AsuC2I CCSGG 5 cut(s) 655, 791, 1037, 1231, 1910
AsuHPI GGTGA 3 cut(s) 694, 1357, 1535
AsuII TTCGAA 1 cut(s) 1205
AvaI CYCGRG 4 cut(s) 197, 386, 772, 1567
AvaII GGWCC 4 cut(s) 447, 1039, 1122, 1141
AxyI CCTNAGG 1 cut(s) 1641
BaeGI GKGCMC 2 cut(s) 211, 911
BalI TGGCCA 3 cut(s) 1154, 1919, 1931
BanI GGYRCC 2 cut(s) 906, 1771
BanII GRGCYC 1 cut(s) 211
BauI CACGAG 2 cut(s) 809, 1268
BbsI GAAGAC 1 cut(s) 1241
Bbv12I GWGCWC 1 cut(s) 1258
BbvCI CCTCAGC 1 cut(s) 1046
BbvI GCAGC 7 cut(s) 311, 604, 1027, 1248, 1369, 1407, 1494
BccI CCATC 4 cut(s) 1295, 1324, 1889, 1940
BceAI ACGGC 4 cut(s) 312, 459, 752, 1450
BcgI CGANNNNNNTGC 4 cut(s) 941, 975, 1259, 1293
BciT130I CCWGG 3 cut(s) 205, 606, 1836
BcnI CCSGG 5 cut(s) 655, 791, 1037, 1231, 1910
BcoDI GTCTC 5 cut(s) 28, 564, 775, 823, 1709
BfaI CTAG 4 cut(s) 335, 407, 510, 522
BmcAI AGTACT 1 cut(s) 1708
Bme1390I CCNGG 8 cut(s) 205, 606, 655, 791, 1037, 1231, 1836, 1910
Bme18I GGWCC 4 cut(s) 447, 1039, 1122, 1141
BmeRI GACNNNNNGTC 1 cut(s) 169
BmeT110I CYCGRG 4 cut(s) 197, 386, 772, 1567
BmgBI CACGTC 1 cut(s) 1387
BmgT120I GGNCC 6 cut(s) 207, 208, 447, 1039, 1122, 1141
BmrFI CCNGG 8 cut(s) 205, 606, 655, 791, 1037, 1231, 1836, 1910
BmsI GCATC 2 cut(s) 162, 1210
BpiI GAAGAC 1 cut(s) 1241
BplI GAGNNNNNCTC 4 cut(s) 86, 118, 803, 835
BpmI CTGGAG 1 cut(s) 1103
Bpu10I CCTNAGC 2 cut(s) 1046, 1503
Bpu14I TTCGAA 1 cut(s) 1205
BpuEI CTTGAG 4 cut(s) 215, 487, 499, 517
BpuMI CCSGG 5 cut(s) 655, 791, 1037, 1231, 1910
BsaBI GATNNNNATC 1 cut(s) 1947
BsaHI GRCGYC 1 cut(s) 255
BsaI GGTCTC 1 cut(s) 28
BsaJI CCNNGG 9 cut(s) 204, 250, 259, 665, 1149, 1431, 1466, 1835, 1920
BsaWI WCCGGW 4 cut(s) 160, 798, 991, 1090
BsaXI ACNNNNNCTCC 8 cut(s) 61, 91, 104, 134, 592, 622, 1304, 1334
Bsc4I CCNNNNNNNGG 9 cut(s) 83, 204, 366, 594, 630, 903, 1150, 1472, 1915
Bse118I RCCGGY 4 cut(s) 399, 798, 1041, 1250
Bse1I ACTGG 2 cut(s) 271, 1962
Bse21I CCTNAGG 1 cut(s) 1641
Bse3DI GCAATG 3 cut(s) 1242, 1275, 1851
Bse8I GATNNNNATC 1 cut(s) 1947
BseAI TCCGGA 1 cut(s) 991
BseBI CCWGG 3 cut(s) 205, 606, 1836
BseDI CCNNGG 9 cut(s) 204, 250, 259, 665, 1149, 1431, 1466, 1835, 1920
BseGI GGATG 3 cut(s) 1060, 1335, 1423
BseJI GATNNNNATC 1 cut(s) 1947
BseLI CCNNNNNNNGG 9 cut(s) 83, 204, 366, 594, 630, 903, 1150, 1472, 1915
BseMI GCAATG 3 cut(s) 1242, 1275, 1851
BseMII CTCAG 6 cut(s) 632, 1037, 1494, 1632, 1787, 1790
BseNI ACTGG 2 cut(s) 271, 1962
BseRI GAGGAG 4 cut(s) 109, 686, 935, 1753
BseSI GKGCMC 2 cut(s) 211, 911
BseX3I CGGCCG 1 cut(s) 1248
BseXI GCAGC 7 cut(s) 311, 604, 1027, 1248, 1369, 1407, 1494
Bsh1236I CGCG 5 cut(s) 252, 261, 786, 816, 936
Bsh1285I CGRYCG 4 cut(s) 584, 784, 981, 1251
BshNI GGYRCC 2 cut(s) 906, 1771
BshTI ACCGGT 1 cut(s) 798
BsiEI CGRYCG 4 cut(s) 584, 784, 981, 1251
BsiHKAI GWGCWC 1 cut(s) 1258
BsiHKCI CYCGRG 4 cut(s) 197, 386, 772, 1567
BslFI GGGAC 4 cut(s) 99, 1052, 1108, 1127
BslI CCNNNNNNNGG 9 cut(s) 83, 204, 366, 594, 630, 903, 1150, 1472, 1915
BsmAI GTCTC 5 cut(s) 28, 564, 775, 823, 1709
BsmBI CGTCTC 4 cut(s) 564, 775, 823, 1709
BsmFI GGGAC 4 cut(s) 99, 1052, 1108, 1127
Bso31I GGTCTC 1 cut(s) 28
BsoBI CYCGRG 4 cut(s) 197, 386, 772, 1567
Bsp119I TTCGAA 1 cut(s) 1205
Bsp120I GGGCCC 1 cut(s) 207
Bsp1286I GDGCHC 3 cut(s) 211, 911, 1258
Bsp13I TCCGGA 1 cut(s) 991
Bsp143I GATC 8 cut(s) 563, 575, 781, 823, 931, 978, 1272, 1600
Bsp19I CCATGG 1 cut(s) 1920
Bsp68I TCGCGA 1 cut(s) 786
BspCNI CTCAG 6 cut(s) 633, 1038, 1495, 1633, 1788, 1789
BspEI TCCGGA 1 cut(s) 991
BspFNI CGCG 5 cut(s) 252, 261, 786, 816, 936
BspHI TCATGA 1 cut(s) 1699
BspPI GGATC 1 cut(s) 831
BspT104I TTCGAA 1 cut(s) 1205
BspT107I GGYRCC 2 cut(s) 906, 1771
BspTNI GGTCTC 1 cut(s) 28
BsrBI CCGCTC 1 cut(s) 676
BsrDI GCAATG 3 cut(s) 1242, 1275, 1851
BsrFI RCCGGY 4 cut(s) 399, 798, 1041, 1250
BsrI ACTGG 2 cut(s) 271, 1962
BssAI RCCGGY 4 cut(s) 399, 798, 1041, 1250
BssECI CCNNGG 9 cut(s) 204, 250, 259, 665, 1149, 1431, 1466, 1835, 1920
BssMI GATC 8 cut(s) 563, 575, 781, 823, 931, 978, 1272, 1600
BssNI GRCGYC 1 cut(s) 255
BssSI CACGAG 2 cut(s) 809, 1268
BssT1I CCWWGG 2 cut(s) 1431, 1920
Bst2BI CACGAG 2 cut(s) 809, 1268
Bst2UI CCWGG 3 cut(s) 205, 606, 1836
Bst4CI ACNGT 1 cut(s) 554
Bst6I CTCTTC 3 cut(s) 149, 629, 1319
BstACI GRCGYC 1 cut(s) 255
BstAPI GCANNNNNTGC 1 cut(s) 132
BstBI TTCGAA 1 cut(s) 1205
BstC8I GCNNGC 2 cut(s) 340, 838
BstDEI CTNAG 7 cut(s) 641, 1046, 1503, 1610, 1641, 1776, 1796
BstDSI CCRYGG 5 cut(s) 250, 259, 1149, 1466, 1920
BstF5I GGATG 3 cut(s) 1060, 1335, 1423
BstFNI CGCG 5 cut(s) 252, 261, 786, 816, 936
BstHHI GCGC 2 cut(s) 816, 938
BstKTI GATC 8 cut(s) 566, 578, 784, 826, 934, 981, 1275, 1603
BstMAI GTCTC 5 cut(s) 28, 564, 775, 823, 1709
BstMBI GATC 8 cut(s) 563, 575, 781, 823, 931, 978, 1272, 1600
BstMCI CGRYCG 4 cut(s) 584, 784, 981, 1251
BstNI CCWGG 3 cut(s) 205, 606, 1836
BstSCI CCNGG 8 cut(s) 203, 604, 653, 789, 1035, 1229, 1834, 1908
BstSLI GKGCMC 2 cut(s) 211, 911
BstUI CGCG 5 cut(s) 252, 261, 786, 816, 936
BstV1I GCAGC 7 cut(s) 311, 604, 1027, 1248, 1369, 1407, 1494
BstV2I GAAGAC 1 cut(s) 1241
BstX2I RGATCY 1 cut(s) 823
BstXI CCANNNNNNTGG 1 cut(s) 1927
BstYI RGATCY 1 cut(s) 823
BstZI CGGCCG 1 cut(s) 1248
Bsu36I CCTNAGG 1 cut(s) 1641
BtgI CCRYGG 5 cut(s) 250, 259, 1149, 1466, 1920
BtgZI GCGATG 1 cut(s) 1956
BtrI CACGTC 1 cut(s) 1387
BtsCI GGATG 3 cut(s) 1060, 1335, 1423
BtsI GCAGTG 1 cut(s) 1303
BtsIMutI CAGTG 4 cut(s) 264, 747, 1303, 1325
BtuMI TCGCGA 1 cut(s) 786
Cac8I GCNNGC 2 cut(s) 340, 838
CaiI CAGNNNCTG 1 cut(s) 1957
CciI TCATGA 1 cut(s) 1699
CfoI GCGC 2 cut(s) 816, 938
Cfr10I RCCGGY 4 cut(s) 399, 798, 1041, 1250
Cfr13I GGNCC 6 cut(s) 207, 208, 447, 1039, 1122, 1141
Cfr42I CCGCGG 2 cut(s) 253, 262
CseI GACGC 3 cut(s) 96, 244, 805
Csp6I GTAC 4 cut(s) 158, 555, 1545, 1707
CspAI ACCGGT 1 cut(s) 798
CviAII CATG 7 cut(s) 451, 754, 1156, 1399, 1678, 1700, 1921
CviQI GTAC 4 cut(s) 158, 555, 1545, 1707
DdeI CTNAG 7 cut(s) 641, 1046, 1503, 1610, 1641, 1776, 1796
DpnI GATC 8 cut(s) 565, 577, 783, 825, 933, 980, 1274, 1602
DpnII GATC 8 cut(s) 563, 575, 781, 823, 931, 978, 1272, 1600
DriI GACNNNNNGTC 1 cut(s) 169
EaeI YGGCCR 4 cut(s) 1152, 1248, 1917, 1929
EagI CGGCCG 1 cut(s) 1248
Eam1104I CTCTTC 3 cut(s) 149, 629, 1319
Eam1105I GACNNNNNGTC 1 cut(s) 169
EarI CTCTTC 3 cut(s) 149, 629, 1319
EciI GGCGGA 3 cut(s) 94, 613, 1325
EclXI CGGCCG 1 cut(s) 1248
Eco130I CCWWGG 2 cut(s) 1431, 1920
Eco24I GRGCYC 1 cut(s) 211
Eco31I GGTCTC 1 cut(s) 28
Eco47I GGWCC 4 cut(s) 447, 1039, 1122, 1141
Eco52I CGGCCG 1 cut(s) 1248
Eco57I CTGAAG 2 cut(s) 1301, 1382
Eco81I CCTNAGG 1 cut(s) 1641
Eco88I CYCGRG 4 cut(s) 197, 386, 772, 1567
EcoO109I RGGNCCY 2 cut(s) 1122, 1141
EcoRI GAATTC 1 cut(s) 1285
EcoRII CCWGG 3 cut(s) 203, 604, 1834
EcoT14I CCWWGG 2 cut(s) 1431, 1920
EcoT38I GRGCYC 1 cut(s) 211
ErhI CCWWGG 2 cut(s) 1431, 1920
Esp3I CGTCTC 4 cut(s) 564, 775, 823, 1709
FaeI CATG 7 cut(s) 454, 757, 1159, 1402, 1681, 1703, 1924
FalI AAGNNNNNCTT 6 cut(s) 1355, 1387, 1616, 1648, 1868, 1900
FaqI GGGAC 4 cut(s) 99, 1052, 1108, 1127
FatI CATG 7 cut(s) 450, 753, 1155, 1398, 1677, 1699, 1920
FauI CCCGC 3 cut(s) 476, 597, 669
FblI GTMKAC 2 cut(s) 1811, 1968
FokI GGATG 3 cut(s) 1067, 1342, 1430
FriOI GRGCYC 1 cut(s) 211
FspBI CTAG 4 cut(s) 335, 407, 510, 522
GlaI GCGC 2 cut(s) 815, 937
GsuI CTGGAG 1 cut(s) 1103
HgaI GACGC 3 cut(s) 96, 244, 805
HhaI GCGC 2 cut(s) 816, 938
Hin1I GRCGYC 1 cut(s) 255
Hin1II CATG 7 cut(s) 454, 757, 1159, 1402, 1681, 1703, 1924
Hin6I GCGC 2 cut(s) 814, 936
HinP1I GCGC 2 cut(s) 814, 936
HincII GTYRAC 1 cut(s) 1969
HindII GTYRAC 1 cut(s) 1969
HindIII AAGCTT 1 cut(s) 1160
HinfI GANTC 9 cut(s) 50, 200, 391, 920, 983, 1032, 1565, 1732, 1792
HphI GGTGA 3 cut(s) 694, 1357, 1535
Hpy166II GTNNAC 3 cut(s) 1545, 1812, 1969
Hpy8I GTNNAC 3 cut(s) 1545, 1812, 1969
Hpy99I CGWCG 1 cut(s) 694
HpyCH4III ACNGT 1 cut(s) 554
HpyCH4IV ACGT 3 cut(s) 557, 1175, 1386
HpyCH4V TGCA 6 cut(s) 29, 243, 342, 962, 1223, 1510
HpyF3I CTNAG 7 cut(s) 641, 1046, 1503, 1610, 1641, 1776, 1796
HpySE526I ACGT 3 cut(s) 557, 1175, 1386
Hsp92I GRCGYC 1 cut(s) 255
Hsp92II CATG 7 cut(s) 454, 757, 1159, 1402, 1681, 1703, 1924
HspAI GCGC 2 cut(s) 814, 936
KflI GGGWCCC 1 cut(s) 1141
Kpn2I TCCGGA 1 cut(s) 991
KspI CCGCGG 2 cut(s) 253, 262
Kzo9I GATC 8 cut(s) 563, 575, 781, 823, 931, 978, 1272, 1600
LmnI GCTCC 5 cut(s) 315, 661, 673, 1012, 1312
Lsp1109I GCAGC 7 cut(s) 311, 604, 1027, 1248, 1369, 1407, 1494
LweI GCATC 2 cut(s) 162, 1210
MaeI CTAG 4 cut(s) 335, 407, 510, 522
MaeII ACGT 3 cut(s) 557, 1175, 1386
MaeIII GTNAC 6 cut(s) 495, 644, 1086, 1382, 1673, 1819
MalI GATC 8 cut(s) 565, 577, 783, 825, 933, 980, 1274, 1602
MbiI CCGCTC 1 cut(s) 676
MboI GATC 8 cut(s) 563, 575, 781, 823, 931, 978, 1272, 1600
MflI RGATCY 1 cut(s) 823
MhlI GDGCHC 3 cut(s) 211, 911, 1258
MlsI TGGCCA 3 cut(s) 1154, 1919, 1931
MluCI AATT 5 cut(s) 362, 743, 1285, 1451, 1867
MluNI TGGCCA 3 cut(s) 1154, 1919, 1931
MlyI GAGTC 6 cut(s) 44, 209, 914, 992, 1041, 1559
MmeI TCCRAC 1 cut(s) 873
Mox20I TGGCCA 3 cut(s) 1154, 1919, 1931
MroI TCCGGA 1 cut(s) 991
MroXI GAANNNNTTC 3 cut(s) 192, 971, 1880
MscI TGGCCA 3 cut(s) 1154, 1919, 1931
MseI TTAA 5 cut(s) 612, 1070, 1182, 1454, 1634
MslI CAYNNNNRTG 1 cut(s) 752
Msp20I TGGCCA 3 cut(s) 1154, 1919, 1931
MspA1I CMGCKG 4 cut(s) 252, 261, 595, 1360
MspR9I CCNGG 8 cut(s) 205, 606, 655, 791, 1037, 1231, 1836, 1910
MvaI CCWGG 3 cut(s) 205, 606, 1836
MvnI CGCG 5 cut(s) 252, 261, 786, 816, 936
NciI CCSGG 5 cut(s) 655, 791, 1037, 1231, 1910
NcoI CCATGG 1 cut(s) 1920
NdeII GATC 8 cut(s) 563, 575, 781, 823, 931, 978, 1272, 1600
NlaIII CATG 7 cut(s) 454, 757, 1159, 1402, 1681, 1703, 1924
NmeAIII GCCGAG 1 cut(s) 690
NmuCI GTSAC 4 cut(s) 495, 1382, 1673, 1819
NruI TCGCGA 1 cut(s) 786
NspV TTCGAA 1 cut(s) 1205
PaeR7I CTCGAG 1 cut(s) 772
PagI TCATGA 1 cut(s) 1699
PcsI WCGNNNNNNNCGW 2 cut(s) 77, 920
PdmI GAANNNNTTC 3 cut(s) 192, 971, 1880
PfeI GAWTC 3 cut(s) 391, 1732, 1792
PfoI TCCNGGA 1 cut(s) 1035
PinAI ACCGGT 1 cut(s) 798
Ple19I CGATCG 2 cut(s) 784, 981
PleI GAGTC 6 cut(s) 44, 208, 914, 991, 1040, 1559
PpsI GAGTC 6 cut(s) 44, 208, 914, 991, 1040, 1559
PpuMI RGGWCCY 2 cut(s) 1122, 1141
Psp1406I AACGTT 1 cut(s) 1175
Psp5II RGGWCCY 2 cut(s) 1122, 1141
Psp6I CCWGG 3 cut(s) 203, 604, 1834
PspGI CCWGG 3 cut(s) 203, 604, 1834
PspOMI GGGCCC 1 cut(s) 207
PspPI GGNCC 6 cut(s) 207, 208, 447, 1039, 1122, 1141
PspPPI RGGWCCY 2 cut(s) 1122, 1141
PstNI CAGNNNCTG 1 cut(s) 1957
PsuI RGATCY 1 cut(s) 823
PvuI CGATCG 2 cut(s) 784, 981
PvuII CAGCTG 1 cut(s) 1360
RruI TCGCGA 1 cut(s) 786
RsaI GTAC 4 cut(s) 159, 556, 1546, 1708
RsaNI GTAC 4 cut(s) 158, 555, 1545, 1707
RseI CAYNNNNRTG 1 cut(s) 752
SacII CCGCGG 2 cut(s) 253, 262
SalI GTCGAC 1 cut(s) 1967
SaqAI TTAA 5 cut(s) 612, 1070, 1182, 1454, 1634
Sau3AI GATC 8 cut(s) 563, 575, 781, 823, 931, 978, 1272, 1600
Sau96I GGNCC 6 cut(s) 207, 208, 447, 1039, 1122, 1141
ScaI AGTACT 1 cut(s) 1708
SchI GAGTC 6 cut(s) 44, 209, 914, 992, 1041, 1559
ScrFI CCNGG 8 cut(s) 205, 606, 655, 791, 1037, 1231, 1836, 1910
SduI GDGCHC 3 cut(s) 211, 911, 1258
SfaNI GCATC 2 cut(s) 162, 1210
Sfr274I CTCGAG 1 cut(s) 772
Sfr303I CCGCGG 2 cut(s) 253, 262
SfuI TTCGAA 1 cut(s) 1205
SgrBI CCGCGG 2 cut(s) 253, 262
SinI GGWCC 4 cut(s) 447, 1039, 1122, 1141
SlaI CTCGAG 1 cut(s) 772
SmiMI CAYNNNNRTG 1 cut(s) 752
SmlI CTYRAG 5 cut(s) 230, 502, 514, 532, 772
SmoI CTYRAG 5 cut(s) 230, 502, 514, 532, 772
Sse9I AATT 5 cut(s) 362, 743, 1285, 1451, 1867
SspMI CTAG 4 cut(s) 335, 407, 510, 522
StyD4I CCNGG 8 cut(s) 203, 604, 653, 789, 1035, 1229, 1834, 1908
StyI CCWWGG 2 cut(s) 1431, 1920
TaaI ACNGT 1 cut(s) 554
TaiI ACGT 3 cut(s) 560, 1178, 1389
TaqII GACCGA 1 cut(s) 570
TasI AATT 5 cut(s) 362, 743, 1285, 1451, 1867
TatI WGTACW 2 cut(s) 1544, 1706
TauI GCSGC 8 cut(s) 255, 264, 474, 598, 836, 899, 1250, 1267
TfiI GAWTC 3 cut(s) 391, 1732, 1792
Tru1I TTAA 5 cut(s) 612, 1070, 1182, 1454, 1634
Tru9I TTAA 5 cut(s) 612, 1070, 1182, 1454, 1634
TscAI CASTG 4 cut(s) 271, 754, 1303, 1325
TseFI GTSAC 4 cut(s) 495, 1382, 1673, 1819
TseI GCWGC 7 cut(s) 299, 592, 1015, 1261, 1357, 1395, 1507
Tsp45I GTSAC 4 cut(s) 495, 1382, 1673, 1819
TspDTI ATGAA 2 cut(s) 467, 1172
TspGWI ACGGA 6 cut(s) 69, 483, 707, 1138, 1733, 1838
TspRI CASTG 4 cut(s) 271, 754, 1303, 1325
VpaK11BI GGWCC 4 cut(s) 447, 1039, 1122, 1141
XapI RAATTY 3 cut(s) 743, 1285, 1867
XbaI TCTAGA 1 cut(s) 406
XhoI CTCGAG 1 cut(s) 772
XmiI GTMKAC 2 cut(s) 1811, 1968
XmnI GAANNNNTTC 3 cut(s) 192, 971, 1880
XspI CTAG 4 cut(s) 335, 407, 510, 522
ZrmI AGTACT 1 cut(s) 1708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.