Rh4AG290400

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000255
Physical Location & Seq
Reverse (-)
60421575 .. 60426370
4796 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG290400.1

Sequence Viewer

Length: 1962 bp
ATGGCTCCTCCCAGTCTTCTCGGTCCTCCCGAGCTCCACAAACCCTCACCCACCACCACCACCGCTCCGCCACCACCCCAACCCGAATCCAAACCCGCCACCGGCGAACCCTTCGTGGACCTGATGGTGGCGAATTTCAACGACGTCGGAACCAAGCACGACCTCCCGATGGGACTGACGGAGAACGCCTCCGCCACGTTTCTCTCCACCGGCAACCCCTGCCTCGATCTCTTCTTCCACGTCGTCCCGAACACTCCGGCCGACTACCTTACCGAGCAGCTCCCCAAGGCCTGGGACCGCGACGCCCTCACCACGCTCAAGCTCATCTGCAACCTCCGTGGCGTGCGTGGCACCGGGAAATCCGACAAGGAAGGGTTCCACACGGCGGCGTTTTGGCTCCACAAGCACCACCCCAAAACCCTCGCCTGCAACCTCGCGTCCTTCGCCGAGTTCGGTTACTTCAAGGACCTGCCGGAGATCGTGTACCGGCTCCTGGAGGGCGACGACGTGAGGAAGAAACAGAAGGCGGAGTGGCGGGAGAGGAAACGCGGCGGAGGCAGGAAGCGACGCGAGTCGAGTCAGGAACCCGGATCCGACGGCGAAACCGGGTCGAGGTCCGAGAAGAAGAGAATCAGGAGCGAGGTGCCGAAAGAGGAGAGGGAGAAGAAGGCCAAGGAGTGGCAGATGGGGGAGAAGGCCAAGGCGAGCGGGTTGAGGAAGGAGAAGAAGATCGCCATGGCCAAGAAGGTCGTGGTCCGGTACGAGCGCGACCCGGATTTCCGGTTCCTTCACGACCGGGTCTCGGATCTTTTCGCCGAGTGCTTGAAATCCGATATGGAGAATCTCAAGTCCAATCAGCTCAACAAGATAACCCTAGCGGCCAAGTGGTGCCCTTCGATTGACTCCTCATTCGATCGGGCCACTTTGATCTGTGAGAGCGTTGCGAAGAAGATTTTCCCGCGCGAATCGAACAAGGAATATGAAGGCGTGGAGGAGTCACATTATGCTTACAGAGTGAGGGACCGGCTGAGGAAGGAGGTGCTGGTGCCGCTGAGGAAGGCCTTGGAGTTGCCGGAGATTTACATGGGGGCTAACCAGTGGGAGAAGATTCCGTATAACAGAGTGGCCTCTGTGGCAATGAAGCTGTACAAGGACAAGTTCAAGAAGCACGATGAGGAGAGGTTTAATAAGTATTTGGAGGATGTGAAAGGCGGGAAGGCTAAGATTGCGGCGGGAGCTCTGCTTCCGCATGAGATTATTGAGTCTCTGAATGATGGGGAGGGTGATGATGGGCAAGTAGCGGAGCTTCAGTGGAAGAGAATGGTGGAGGATATGTTGAAGTTGGGGAAGATGAACAACTGTTTGGCTGTGTGTGATGTGAGTGGGAGCATGAGTGGGACTCCGATGGAGGTGGCGGTGGCGTTGGGGCTGTTGGTTTCGGAGTTGAGTGAGGACCCTTGGAAAGGGTTGGTGGTCAGTTTCAGTGCGGATCCTCAGCTGCATTTGGTGAAGGGGGAGAGTCTTAAGGACAAGACTGATTTCATCACGAAAATGGATTGGGGGATGAACACTAATTTCCAGAAGGTGTTTGATTTGCTTCTGAAAGTGGCTGTCAAGGGGAATTTGAAGCCGGAGAATATGATCAAGAGGCTGTTTGTGTTCAGTGACATGGAGTTTGATCAGGCTTCGGAAAATGAGTGGGAGACTGATTATGAGGTGATAGAGAAGAAGTTTAAGGAGGCCGGGTATGGGGATGTGATTCCGCAGTTGGTGTTTTGGAATCTTAGGCACTCCAGGTCTATCCCTGTGCCTGGGAATCAGAAAGGCACTGCTCTGTTGAGTGGATTCTCTAAGAACTTGCTGAAACTCTTTATGGATAATGATGGGGAAGTTCAGCCGGATATGTTCATGGATTTGGCCATTGCTGGACCAGAGTATCAGAAGCTGGTTGTGCTGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

653

Amino Acids

73.91

Weight (kDa)

7.2

Isoelectric Point (pI)

36.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2828 PF11443 60 - 451 3.9e-142 Domain of unknown function (DUF2828)
DUF7788 PF25043 453 - 631 2.5e-79 Domain of unknown function (DUF7788)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000255)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24780 AT5G13210 AT5G43390 AT5G43400
fragaria_vesca FvH4_4g23010 FvH4_5g12462 FvH4_5g12490 FvH4_5g12491 FvH4_5g20030 FvH4_5g20031 FvH4_5g21510 FvH4_6g30070
malus_domestica MD00G1095100.v1.1 MD05G1186800.v1.1 MD05G1259500.v1.1 MD05G1260200.v1.1 MD06G1159400.v1.1 MD06G1159500.v1.1 MD06G1162500.v1.1 MD06G1164600.v1.1 MD06G1187800.v1.1 MD06G1212600.v1.1 MD06G1212700.v1.1 MD13G1126800.v1.1 MD14G1168300.v1.1 MD14G1206600.v1.1 MD14G1223900.v1.1 MD14G1230800.v1.1 MD15G1240300.v1.1 MD15G1416800.v1.1 MD16G1127300.v1.1
prunus_persica Prupe.1G218700_v2.0.a1 Prupe.4G045100_v2.0.a1 Prupe.5G159300_v2.0.a1 Prupe.5G172200_v2.0.a1 Prupe.5G172300_v2.0.a1 Prupe.5G173200_v2.0.a1 Prupe.5G173300_v2.0.a1 Prupe.5G173400_v2.0.a1 Prupe.5G219000_v2.0.a1 Prupe.5G219100_v2.0.a1 Prupe.5G219200_v2.0.a1
pyrus_communis pycom05g24390 pycom06g19020 pycom10g15060 pycom10g24800 pycom13g11010 pycom16g10800 pycom16g10810 pycom17g22350 pycom17g22360
rosa_chinensis RchiOBHm_Chr2g0136111 RchiOBHm_Chr4g0429291 RchiOBHm_Chr7g0184981 RchiOBHm_Chr7g0184991 RchiOBHm_Chr7g0185001 RchiOBHm_Chr7g0204841
rosa_laevigata RLG00000003427 RLG00000004941 RLG00000004943 RLG00000004944 RLG00000004945 RLG00000004946 RLG00000007084 RLG00000013701 RLG00000019505 RLG00000019506
rosa_multiflora Rmu_co8294559.1_g000001 Rmu_co8375195.1_g000001 Rmu_co8450783.1_g000001 Rmu_co8519567.1_g000001 Rmu_sc0000054.1_g000008 Rmu_sc0001689.1_g000017 Rmu_sc0001801.1_g000027 Rmu_sc0002070.1_g000010 Rmu_sc0002180.1_g000022 Rmu_sc0002986.1_g000018 Rmu_sc0003221.1_g000027 Rmu_sc0005629.1_g000018 Rmu_sc0009898.1_g000002 Rmu_sc0018428.1_g000003 Rmu_sc0018428.1_g000005 Rmu_sc0022816.1_g000001 Rmu_sc0041781.1_g000001 Rmu_ssc0000289.1_g000007 Rmu_ssc0000289.1_g000008 Rmu_ssc0000289.1_g000010
rosa_roxburghii Rroxscaffold_2G00108460 Rroxscaffold_3G00252720 Rroxscaffold_3G00269290 Rroxscaffold_5G00349680 Rroxscaffold_5G00370900 Rroxscaffold_6G00415020
rosa_rugosa Rorug01G0043400 Rorug02G0328200 Rorug04G0234800 Rorug06G0465600 Rorug06G0465800 Rorug06G0465900 Rorug06G0466000 Rorug06G0466100 Rorug07G0086200 Rorug07G0086200
rosa_samantha Rh2BG386700 Rh2CG365900 Rh2DG402800 Rh4AG290400 Rh4BG296100 Rh4CG311900 Rh4DG293300 Rh7AG077000 Rh7AG077100 Rh7AG077200 Rh7AG216600 Rh7BG070200 Rh7BG070300 Rh7BG070400 Rh7BG213700 Rh7CG071400 Rh7CG071600 Rh7CG071700 Rh7CG071900 Rh7CG230100 Rh7DG070800 Rh7DG070900 Rh7DG224200 Rh7DG440100
rosa_wichuraiana Rw2G030970 Rw4G025150 Rw7G005840 Rw7G005850 Rw7G005860 Rw7G018770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 147
Acc36I ACCTGC 1 cut(s) 477
AccB1I GGYRCC 4 cut(s) 350, 643, 888, 1045
AccB7I CCANNNNNTGG 2 cut(s) 291, 678
AccBSI CCGCTC 2 cut(s) 65, 708
AccII CGCG 7 cut(s) 300, 437, 549, 570, 768, 961, 963
AclWI GGATC 5 cut(s) 585, 598, 813, 1484, 1497
AcoI YGGCCR 4 cut(s) 258, 738, 879, 1917
AcsI RAATTY 2 cut(s) 133, 1621
AcuI CTGAAG 1 cut(s) 1292
AcyI GRCGYC 2 cut(s) 144, 303
AfaI GTAC 3 cut(s) 485, 761, 1148
AflII CTTAAG 1 cut(s) 1523
AgsI TTSAA 6 cut(s) 139, 463, 826, 1162, 1339, 1627
AjiI CACGTC 2 cut(s) 241, 508
AjnI CCWGG 4 cut(s) 290, 492, 1793, 1810
AjuI GAANNNNNNNTTGG 2 cut(s) 1346, 1378
AloI GAACNNNNNNTCC 2 cut(s) 767, 799
AluBI AGCT 9 cut(s) 34, 280, 322, 859, 1144, 1238, 1306, 1498, 1945
AluI AGCT 9 cut(s) 34, 280, 322, 859, 1144, 1238, 1306, 1498, 1945
Alw21I GWGCWC 2 cut(s) 36, 1240
Alw26I GTCTC 3 cut(s) 805, 1269, 1697
AlwI GGATC 5 cut(s) 585, 598, 813, 1484, 1497
AlwNI CAGNNNCTG 1 cut(s) 1945
Ama87I CYCGRG 1 cut(s) 29
ApeKI GCWGC 2 cut(s) 277, 1498
ApoI RAATTY 2 cut(s) 133, 1621
ArsI GACNNNNNNTTYG 4 cut(s) 893, 925, 1658, 1690
Asp700I GAANNNNTTC 1 cut(s) 953
AspLEI GCGC 2 cut(s) 768, 963
AsuC2I CCSGG 6 cut(s) 355, 588, 607, 773, 797, 1744
AsuHPI GGTGA 5 cut(s) 39, 301, 1295, 1519, 1729
AvaI CYCGRG 1 cut(s) 29
AvaII GGWCC 9 cut(s) 23, 118, 295, 466, 615, 754, 1021, 1453, 1928
BaeGI GKGCMC 1 cut(s) 893
BaeI ACNNNNGTAYC 2 cut(s) 1919, 1952
BalI TGGCCA 2 cut(s) 740, 1919
BamHI GGATCC 2 cut(s) 590, 1489
BanI GGYRCC 4 cut(s) 350, 643, 888, 1045
BanII GRGCYC 2 cut(s) 36, 1240
BarI GAAGNNNNNNTAC 2 cut(s) 1290, 1322
BbsI GAAGAC 1 cut(s) 8
Bbv12I GWGCWC 2 cut(s) 36, 1240
BbvCI CCTCAGC 3 cut(s) 1028, 1052, 1494
BbvI GCAGC 2 cut(s) 289, 1485
BccI CCATC 7 cut(s) 118, 163, 679, 1268, 1283, 1399, 1877
BceAI ACGGC 2 cut(s) 399, 613
BciT130I CCWGG 4 cut(s) 292, 494, 1795, 1812
BclI TGATCA 2 cut(s) 1641, 1678
BcnI CCSGG 6 cut(s) 355, 588, 607, 773, 797, 1744
BcoDI GTCTC 3 cut(s) 805, 1269, 1697
BfaI CTAG 1 cut(s) 875
BfrI CTTAAG 1 cut(s) 1523
BfuAI ACCTGC 1 cut(s) 477
BglI GCCNNNNNGGC 1 cut(s) 1133
BisI GCNGC 7 cut(s) 278, 387, 550, 879, 1049, 1230, 1499
BlsI GCNGC 7 cut(s) 279, 388, 551, 880, 1050, 1231, 1500
Bme18I GGWCC 9 cut(s) 23, 118, 295, 466, 615, 754, 1021, 1453, 1928
BmeT110I CYCGRG 1 cut(s) 29
BmgBI CACGTC 2 cut(s) 241, 508
BmrI ACTGGG 1 cut(s) 6
BmuI ACTGGG 1 cut(s) 6
BoxI GACNNNNGTC 1 cut(s) 571
BpiI GAAGAC 1 cut(s) 8
BplI GAGNNNNNCTC 4 cut(s) 173, 205, 1384, 1416
BpmI CTGGAG 2 cut(s) 515, 1777
Bpu10I CCTNAGC 3 cut(s) 1028, 1052, 1494
BpuEI CTTGAG 2 cut(s) 302, 830
BpuMI CCSGG 6 cut(s) 355, 588, 607, 773, 797, 1744
BsaHI GRCGYC 2 cut(s) 144, 303
BsaI GGTCTC 1 cut(s) 805
BsaJI CCNNGG 9 cut(s) 285, 291, 337, 672, 699, 735, 1062, 1457, 1811
BsaWI WCCGGW 2 cut(s) 756, 780
Bse118I RCCGGY 4 cut(s) 101, 209, 486, 1023
Bse1I ACTGG 2 cut(s) 12, 1096
Bse3DI GCAATG 2 cut(s) 1143, 1920
BseBI CCWGG 4 cut(s) 292, 494, 1795, 1812
BseDI CCNNGG 9 cut(s) 285, 291, 337, 672, 699, 735, 1062, 1457, 1811
BseGI GGATG 3 cut(s) 1207, 1569, 1759
BseMI GCAATG 2 cut(s) 1143, 1920
BseMII CTCAG 3 cut(s) 1019, 1043, 1508
BseNI ACTGG 2 cut(s) 12, 1096
BseRI GAGGAG 4 cut(s) 668, 895, 1007, 1190
BseSI GKGCMC 1 cut(s) 893
BseX3I CGGCCG 1 cut(s) 258
BseXI GCAGC 2 cut(s) 289, 1485
Bsh1236I CGCG 7 cut(s) 300, 437, 549, 570, 768, 961, 963
Bsh1285I CGRYCG 3 cut(s) 261, 796, 916
BshNI GGYRCC 4 cut(s) 350, 643, 888, 1045
BsiEI CGRYCG 3 cut(s) 261, 796, 916
BsiHKAI GWGCWC 2 cut(s) 36, 1240
BsiHKCI CYCGRG 1 cut(s) 29
BslFI GGGAC 5 cut(s) 186, 230, 308, 1034, 1411
BsmAI GTCTC 3 cut(s) 805, 1269, 1697
BsmFI GGGAC 5 cut(s) 186, 230, 308, 1034, 1411
Bso31I GGTCTC 1 cut(s) 805
BsoBI CYCGRG 1 cut(s) 29
Bsp1286I GDGCHC 3 cut(s) 36, 893, 1240
Bsp1407I TGTACA 1 cut(s) 1146
Bsp19I CCATGG 1 cut(s) 735
BspCNI CTCAG 3 cut(s) 1020, 1044, 1507
BspFNI CGCG 7 cut(s) 300, 437, 549, 570, 768, 961, 963
BspMI ACCTGC 1 cut(s) 477
BspPI GGATC 5 cut(s) 585, 598, 813, 1484, 1497
BspT107I GGYRCC 4 cut(s) 350, 643, 888, 1045
BspTI CTTAAG 1 cut(s) 1523
BspTNI GGTCTC 1 cut(s) 805
BsrBI CCGCTC 2 cut(s) 65, 708
BsrDI GCAATG 2 cut(s) 1143, 1920
BsrFI RCCGGY 4 cut(s) 101, 209, 486, 1023
BsrGI TGTACA 1 cut(s) 1146
BsrI ACTGG 2 cut(s) 12, 1096
BssAI RCCGGY 4 cut(s) 101, 209, 486, 1023
BssECI CCNNGG 9 cut(s) 285, 291, 337, 672, 699, 735, 1062, 1457, 1811
BssNI GRCGYC 2 cut(s) 144, 303
BssT1I CCWWGG 6 cut(s) 285, 672, 699, 735, 1062, 1457
Bst2UI CCWGG 4 cut(s) 292, 494, 1795, 1812
Bst4CI ACNGT 1 cut(s) 1361
Bst6I CTCTTC 3 cut(s) 236, 620, 1310
BstACI GRCGYC 2 cut(s) 144, 303
BstAFI CTTAAG 1 cut(s) 1523
BstAPI GCANNNNNTGC 1 cut(s) 219
BstAUI TGTACA 1 cut(s) 1146
BstC8I GCNNGC 3 cut(s) 344, 427, 706
BstDEI CTNAG 6 cut(s) 1028, 1052, 1221, 1494, 1784, 1851
BstDSI CCRYGG 2 cut(s) 337, 735
BstENI CCTNNNNNAGG 1 cut(s) 1461
BstF5I GGATG 3 cut(s) 1207, 1569, 1759
BstFNI CGCG 7 cut(s) 300, 437, 549, 570, 768, 961, 963
BstHHI GCGC 2 cut(s) 768, 963
BstMAI GTCTC 3 cut(s) 805, 1269, 1697
BstMCI CGRYCG 3 cut(s) 261, 796, 916
BstNI CCWGG 4 cut(s) 292, 494, 1795, 1812
BstPAI GACNNNNGTC 1 cut(s) 571
BstSLI GKGCMC 1 cut(s) 893
BstUI CGCG 7 cut(s) 300, 437, 549, 570, 768, 961, 963
BstV1I GCAGC 2 cut(s) 289, 1485
BstV2I GAAGAC 1 cut(s) 8
BstX2I RGATCY 3 cut(s) 590, 805, 1489
BstYI RGATCY 3 cut(s) 590, 805, 1489
BstZI CGGCCG 1 cut(s) 258
BtgI CCRYGG 2 cut(s) 337, 735
BtrI CACGTC 2 cut(s) 241, 508
BtsCI GGATG 3 cut(s) 1207, 1569, 1759
BtsI GCAGTG 1 cut(s) 1827
BtsIMutI CAGTG 5 cut(s) 1103, 1316, 1489, 1669, 1827
BveI ACCTGC 1 cut(s) 477
Cac8I GCNNGC 3 cut(s) 344, 427, 706
CaiI CAGNNNCTG 1 cut(s) 1945
CfoI GCGC 2 cut(s) 768, 963
Cfr10I RCCGGY 4 cut(s) 101, 209, 486, 1023
CseI GACGC 3 cut(s) 311, 426, 576
Csp6I GTAC 3 cut(s) 484, 760, 1147
CspCI CAANNNNNGTGG 2 cut(s) 319, 354
CviAII CATG 6 cut(s) 736, 1084, 1250, 1390, 1669, 1909
CviQI GTAC 3 cut(s) 484, 760, 1147
DdeI CTNAG 6 cut(s) 1028, 1052, 1221, 1494, 1784, 1851
EaeI YGGCCR 4 cut(s) 258, 738, 879, 1917
EagI CGGCCG 1 cut(s) 258
Eam1104I CTCTTC 3 cut(s) 236, 620, 1310
EarI CTCTTC 3 cut(s) 236, 620, 1310
EciI GGCGGA 4 cut(s) 57, 181, 542, 567
Ecl136II GAGCTC 2 cut(s) 34, 1238
EclXI CGGCCG 1 cut(s) 258
Eco130I CCWWGG 6 cut(s) 285, 672, 699, 735, 1062, 1457
Eco147I AGGCCT 2 cut(s) 290, 1061
Eco24I GRGCYC 2 cut(s) 36, 1240
Eco31I GGTCTC 1 cut(s) 805
Eco47I GGWCC 9 cut(s) 23, 118, 295, 466, 615, 754, 1021, 1453, 1928
Eco52I CGGCCG 1 cut(s) 258
Eco53kI GAGCTC 2 cut(s) 34, 1238
Eco57I CTGAAG 1 cut(s) 1292
Eco88I CYCGRG 1 cut(s) 29
EcoICRI GAGCTC 2 cut(s) 34, 1238
EcoNI CCTNNNNNAGG 1 cut(s) 1461
EcoO109I RGGNCCY 2 cut(s) 466, 1453
EcoRII CCWGG 4 cut(s) 290, 492, 1793, 1810
EcoT14I CCWWGG 6 cut(s) 285, 672, 699, 735, 1062, 1457
EcoT38I GRGCYC 2 cut(s) 36, 1240
ErhI CCWWGG 6 cut(s) 285, 672, 699, 735, 1062, 1457
FaeI CATG 6 cut(s) 739, 1087, 1253, 1393, 1672, 1912
FaqI GGGAC 5 cut(s) 186, 230, 308, 1034, 1411
FatI CATG 6 cut(s) 735, 1083, 1249, 1389, 1668, 1908
FauI CCCGC 6 cut(s) 103, 528, 701, 966, 1205, 1225
FbaI TGATCA 2 cut(s) 1641, 1678
Fnu4HI GCNGC 7 cut(s) 278, 387, 550, 879, 1049, 1230, 1499
FokI GGATG 3 cut(s) 1214, 1576, 1766
FriOI GRGCYC 2 cut(s) 36, 1240
Fsp4HI GCNGC 7 cut(s) 278, 387, 550, 879, 1049, 1230, 1499
FspBI CTAG 1 cut(s) 875
GlaI GCGC 2 cut(s) 767, 962
GluI GCNGC 7 cut(s) 278, 387, 550, 879, 1049, 1230, 1499
GsuI CTGGAG 2 cut(s) 515, 1777
HgaI GACGC 3 cut(s) 311, 426, 576
HhaI GCGC 2 cut(s) 768, 963
Hin1I GRCGYC 2 cut(s) 144, 303
Hin1II CATG 6 cut(s) 739, 1087, 1253, 1393, 1672, 1912
Hin6I GCGC 2 cut(s) 766, 961
HinP1I GCGC 2 cut(s) 766, 961
HphI GGTGA 5 cut(s) 39, 301, 1295, 1519, 1729
Hpy166II GTNNAC 2 cut(s) 118, 484
Hpy8I GTNNAC 2 cut(s) 118, 484
Hpy99I CGWCG 8 cut(s) 146, 149, 245, 305, 506, 509, 570, 599
HpyCH4III ACNGT 1 cut(s) 1361
HpyCH4IV ACGT 4 cut(s) 144, 197, 240, 507
HpyCH4V TGCA 3 cut(s) 330, 429, 1501
HpyF3I CTNAG 6 cut(s) 1028, 1052, 1221, 1494, 1784, 1851
HpySE526I ACGT 4 cut(s) 144, 197, 240, 507
Hsp92I GRCGYC 2 cut(s) 144, 303
Hsp92II CATG 6 cut(s) 739, 1087, 1253, 1393, 1672, 1912
HspAI GCGC 2 cut(s) 766, 961
Ksp22I TGATCA 2 cut(s) 1641, 1678
Lsp1109I GCAGC 2 cut(s) 289, 1485
MaeI CTAG 1 cut(s) 875
MaeII ACGT 4 cut(s) 144, 197, 240, 507
MaeIII GTNAC 3 cut(s) 455, 996, 1664
MbiI CCGCTC 2 cut(s) 65, 708
MflI RGATCY 3 cut(s) 590, 805, 1489
MhlI GDGCHC 3 cut(s) 36, 893, 1240
MlsI TGGCCA 2 cut(s) 740, 1919
MluCI AATT 3 cut(s) 133, 1573, 1621
MluNI TGGCCA 2 cut(s) 740, 1919
MlyI GAGTC 7 cut(s) 581, 586, 896, 1004, 1271, 1393, 1528
MmeI TCCRAC 3 cut(s) 127, 387, 618
Mox20I TGGCCA 2 cut(s) 740, 1919
MroXI GAANNNNTTC 1 cut(s) 953
MscI TGGCCA 2 cut(s) 740, 1919
MseI TTAA 3 cut(s) 1185, 1524, 1734
MslI CAYNNNNRTG 1 cut(s) 1550
Msp20I TGGCCA 2 cut(s) 740, 1919
MspA1I CMGCKG 2 cut(s) 1051, 1498
MspCI CTTAAG 1 cut(s) 1523
MvaI CCWGG 4 cut(s) 292, 494, 1795, 1812
MvnI CGCG 7 cut(s) 300, 437, 549, 570, 768, 961, 963
NciI CCSGG 6 cut(s) 355, 588, 607, 773, 797, 1744
NcoI CCATGG 1 cut(s) 735
NlaIII CATG 6 cut(s) 739, 1087, 1253, 1393, 1672, 1912
NmeAIII GCCGAG 2 cut(s) 472, 841
NmuCI GTSAC 2 cut(s) 996, 1664
PceI AGGCCT 2 cut(s) 290, 1061
PcsI WCGNNNNNNNCGW 1 cut(s) 27
PdmI GAANNNNTTC 1 cut(s) 953
PfeI GAWTC 9 cut(s) 86, 630, 841, 965, 1108, 1759, 1780, 1816, 1845
PflFI GACNNNGTC 1 cut(s) 797
PflMI CCANNNNNTGG 2 cut(s) 291, 678
PfoI TCCNGGA 1 cut(s) 492
PkrI GCNGC 7 cut(s) 279, 388, 551, 880, 1050, 1231, 1500
Ple19I CGATCG 1 cut(s) 916
PleI GAGTC 7 cut(s) 580, 585, 896, 1003, 1270, 1393, 1527
PpsI GAGTC 7 cut(s) 580, 585, 896, 1003, 1270, 1393, 1527
PpuMI RGGWCCY 2 cut(s) 466, 1453
PshAI GACNNNNGTC 1 cut(s) 571
Psp124BI GAGCTC 2 cut(s) 36, 1240
Psp5II RGGWCCY 2 cut(s) 466, 1453
Psp6I CCWGG 4 cut(s) 290, 492, 1793, 1810
PspGI CCWGG 4 cut(s) 290, 492, 1793, 1810
PspPPI RGGWCCY 2 cut(s) 466, 1453
PstNI CAGNNNCTG 1 cut(s) 1945
PsuI RGATCY 3 cut(s) 590, 805, 1489
PsyI GACNNNGTC 1 cut(s) 797
PvuI CGATCG 1 cut(s) 916
PvuII CAGCTG 1 cut(s) 1498
RsaI GTAC 3 cut(s) 485, 761, 1148
RsaNI GTAC 3 cut(s) 484, 760, 1147
RseI CAYNNNNRTG 1 cut(s) 1550
SacI GAGCTC 2 cut(s) 36, 1240
SaqAI TTAA 3 cut(s) 1185, 1524, 1734
SatI GCNGC 7 cut(s) 278, 387, 550, 879, 1049, 1230, 1499
SchI GAGTC 7 cut(s) 581, 586, 896, 1004, 1271, 1393, 1528
SduI GDGCHC 3 cut(s) 36, 893, 1240
SgrAI CRCCGGYG 1 cut(s) 101
SinI GGWCC 9 cut(s) 23, 118, 295, 466, 615, 754, 1021, 1453, 1928
SmiMI CAYNNNNRTG 1 cut(s) 1550
SmlI CTYRAG 3 cut(s) 317, 845, 1523
SmoI CTYRAG 3 cut(s) 317, 845, 1523
Sse9I AATT 3 cut(s) 133, 1573, 1621
SseBI AGGCCT 2 cut(s) 290, 1061
SspMI CTAG 1 cut(s) 875
SstI GAGCTC 2 cut(s) 36, 1240
StuI AGGCCT 2 cut(s) 290, 1061
StyI CCWWGG 6 cut(s) 285, 672, 699, 735, 1062, 1457
TaaI ACNGT 1 cut(s) 1361
TaiI ACGT 4 cut(s) 147, 200, 243, 510
TaqI TCGA 6 cut(s) 225, 575, 611, 896, 912, 968
TaqII GACCGA 1 cut(s) 11
TasI AATT 3 cut(s) 133, 1573, 1621
TatI WGTACW 1 cut(s) 1146
TauI GCSGC 5 cut(s) 389, 552, 881, 1051, 1232
TfiI GAWTC 9 cut(s) 86, 630, 841, 965, 1108, 1759, 1780, 1816, 1845
Tru1I TTAA 3 cut(s) 1185, 1524, 1734
Tru9I TTAA 3 cut(s) 1185, 1524, 1734
TscAI CASTG 5 cut(s) 1103, 1316, 1489, 1669, 1834
TseFI GTSAC 2 cut(s) 996, 1664
TseI GCWGC 2 cut(s) 277, 1498
Tsp45I GTSAC 2 cut(s) 996, 1664
TspDTI ATGAA 6 cut(s) 996, 1154, 1367, 1531, 1580, 1897
TspGWI ACGGA 3 cut(s) 194, 326, 1101
TspRI CASTG 5 cut(s) 1103, 1316, 1489, 1669, 1834
Tth111I GACNNNGTC 1 cut(s) 797
Van91I CCANNNNNTGG 2 cut(s) 291, 678
Vha464I CTTAAG 1 cut(s) 1523
VpaK11BI GGWCC 9 cut(s) 23, 118, 295, 466, 615, 754, 1021, 1453, 1928
XagI CCTNNNNNAGG 1 cut(s) 1461
XapI RAATTY 2 cut(s) 133, 1621
XcmI CCANNNNNNNNNTGG 1 cut(s) 748
XmnI GAANNNNTTC 1 cut(s) 953
XspI CTAG 1 cut(s) 875
ZraI GACGTC 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.