FvH4_6g36500

Belongs to the terpene cyclase mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000173
Physical Location & Seq
Forward (+)
28722843 .. 28727509
4667 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g36500.t1

Sequence Viewer

Length: 2295 bp
ATGTGGAAGCTTAAGATTGCAGAAGGCGGGGAAGATTCCTCGCCTTACATATACAGCACAAACAACTTCGTCGGAAGGCAGACGTGGGAGTTCGATCCCGACGCAGGGACTGATGAAGAGCGAGCTGAGGTTGAAGAGGCTCGTCTTCAGTTCTACAACAATCGCTATCAGGTGAAGCCCAGTGGTGACCTTCTGTGGCGGATGCAGTTCCTGAGAGAGAAGAAGTTCAAACCCACAATTCCTCCGGTAAAAATTGAAGATGGCGAGGCTATAACACATGAAAAAACTACAGATGCACTGAGAAGGTCGGTCCACTTCTTCTCAGCGTTGCAGGCAAGCGATGGTCATTGGCCTGCGGAAAATGCCGGCCCTTTGTTTTTCCTTCCTCCTCTGGTCATATGTATGTATATTACAGGGCATCTCAACAGTGTATTCCCCGAGGAGCACCGGAAAGAAATTCTGCGTTACCTATACTATCATCAGAATGAAGATGGTGGCTGGGGACTACACATCGAAGGTCACAGCACCATGTTCAGCACAGGTCTCAGCTACATTTGCATGCGCATTCTCGGAGAAGGACCTGATGATGGTGGCCAAGACAATGCTTGTTCAAGAGCAAGAAAGTGGATTCTTGACCATGGTGGTGTCACACACATGCCCTCTTGGGGAAAGACTTGGCTTTCGATACTTGGGTTGTTTGATTGGTCCGGAAGCAACCCTATGCCCCCAGAGTTTTGGATTCTTCCTTCATTTCTTCCTATGCATCCAGCAAAAATGTGGTGCTATTGTAGGATGGTTTACATGCCAATGTCATACCTATATGGAAAAAGGTTTGTTGGCCCAATTACGCCTCTCATTCTTCAACTGAGGGAAGAACTTTTCATTCAACCTTACAATGAAATCAACTGGAAGAAAGTACGCCATCTATGTGCCAAGGAGGATATCTACTACCCTCATCCTTGGATACAGGATCTCCTTTGGGATAGCCTCTACATATCCACAGAGCCTCTTCTAACTCGTTGGCCCTTCAACAAGTTGATCAGAGAAAAGGCTCTTCAAGTGACAATGAAGCATATTCATTACGAAGATGAGAACAGCCGATACATCACCATTGGATGTGTGGAAAAGGTGTTATGTATGCTTGCTTGTTGGGCTGAAGATCCAAATGGGGACTATTTCAAGAAGCATCTCGCAAGGATCCCGGATTATTTATGGGTTTCTGAGGATGGGATGAAGATGCAAAGTTTTGGTAGTCAGCAATGGGATACTGGTTTTTCCATTCAAGCTCTGCTTGCTACTAATCTAACGGACGAGATTGGACCAGCTCTCGCTAGAGGACATGACTTTATCAAGAAATCTCAGGTCAAGGATAACCCATCTGGTGACTTCAAATGCATGCACCGTCACATTTCTAAAGGATCGTGGACTTTCTCCGATCAAGATCATGGATGGCAGGTATCTGATTGCACTGCAGAAGGTTTAAAGTGTTGCCTTCTTTTGTCGATGATGCCATCGGAGATAGTTGGTGAAAAAATGGAACCTGAGCGCTTGTACGATTCTGTCAACGTTCTACTTTCCCTACAGAGCAAGAATGGTGGTTTATCAGCCTGGGAGCCAGCAGGGGCTGCAGAGTGGTTAGAACTGTTAAATCCGACAGAGTTCTTCGCGGACATTGTGATTGAGCATGAATATGTTGAGTGCACCTCATCTGCAATCCAGGCTTTAGTTCTCTTTAAGAAGCTATACCCTGGCCATAGGAAGAAAGAGATCGAACATTCCATCACCAAATCTATACACTACCTTGAAAATATACAAATGCCGGATGGTTCATGGTATGGAAATTGGGGAGTTTGCTTCACATATGGTACATGGTTTGCGCTAGGAGGATTGGCAGCTGCTGGCGAGACTTTCCGAACAAGCGCAGCTATGCGCAAAGGGGTTAGTTTTCTACTTAAGGCGCAGAGAGAGAACGGTGGTTGGGGAGAGAGTTATCTTTCATGTCCACAAAAGACATACATTCCTTTAGAAGGAAACCGATCCAACTTAGTACATACTGCTTGGGCAATGATGGGTCTCATTCATGCGGGACAGGCAGAAAGAGACCCGACACCTCTTCATCGTGCAGCAAAGTTTATAATCAATTCTCAGCTGGAAAATGGTGATTTTCCCCAGCAGGAAATCACTGGAGTCTTCATGAAGAACTGCATGCTACATTATGCAGCTTATAGAAATATCTACCCACTATGGGCTCTTGCAGAATACCGCAAGCGGGTTCCATTGCCTTCCAAGGCTTGA

Protein Analysis

765

Amino Acids

87.74

Weight (kDa)

6.12

Isoelectric Point (pI)

51.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQHop_cyclase_N PF13249 101 - 405 2e-35 Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C PF13243 420 - 756 1.2e-51 Squalene-hopene cyclase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66960 AT1G66960 AT1G66960 AT1G66960 AT1G78950 AT1G78950 AT1G78955 AT1G78955 AT1G78955 AT1G78955 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78970 AT1G78970 AT1G78970
fragaria_vesca FvH4_5g29480 FvH4_6g36450 FvH4_6g36450 FvH4_6g36500 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g37620
malus_domestica MD03G1089600.v1.1 MD09G1167700.v1.1 MD09G1168200.v1.1 MD09G1168300.v1.1 MD11G1098600.v1.1 MD17G1158300.v1.1 MD17G1158700.v1.1 MD17G1159500.v1.1 MD17G1160500.v1.1 MD17G1182500.v1.1 MD17G1182700.v1.1 MD17G1245800.v1.1 MD17G1246300.v1.1 MD17G1246600.v1.1
prunus_persica Prupe.3G025700_v2.0.a1 Prupe.3G025700_v2.0.a1 Prupe.3G025800_v2.0.a1 Prupe.3G025900_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026400_v2.0.a1 Prupe.3G026500_v2.0.a1 Prupe.3G026700_v2.0.a1 Prupe.3G026800_v2.0.a1
pyrus_communis pycom03g07120 pycom09g08520 pycom09g08590 pycom09g08610 pycom10g10070 pycom11g08310 pycom16g19880 pycom17g15190 pycom17g15300
rosa_chinensis RchiOBHm_Chr2g0136221 RchiOBHm_Chr2g0148451 RchiOBHm_Chr2g0148501 RchiOBHm_Chr2g0148521 RchiOBHm_Chr2g0148661 RchiOBHm_Chr2g0148701 RchiOBHm_Chr2g0148811 RchiOBHm_Chr2g0148941 RchiOBHm_Chr2g0148981 RchiOBHm_Chr2g0150451 RchiOBHm_Chr2g0150521 RchiOBHm_Chr2g0150611 RchiOBHm_Chr4g0420831 RchiOBHm_Chr5g0049791 RchiOBHm_Chr5g0049801
rosa_laevigata RLG00000007735 RLG00000020351 RLG00000020352 RLG00000020357 RLG00000020358 RLG00000020360 RLG00000029093 RLG00000030686
rosa_multiflora Rmu_co8274513.1_g000001 Rmu_sc0000048.1_g000003 Rmu_sc0000048.1_g000033 Rmu_sc0000048.1_g000039 Rmu_sc0001397.1_g000011 Rmu_sc0001998.1_g000039 Rmu_sc0002070.1_g000055 Rmu_sc0002082.1_g000052 Rmu_sc0003181.1_g000010 Rmu_sc0005104.1_g000005 Rmu_sc0006758.1_g000002 Rmu_sc0008411.1_g000029 Rmu_sc0008411.1_g000030 Rmu_sc0017472.1_g000001 Rmu_sc0017472.1_g000003 Rmu_sc0029876.1_g000001 Rmu_ssc0000480.1_g000024 Rmu_ssc0000480.1_g000036
rosa_roxburghii Rroxscaffold_2G00097070 Rroxscaffold_2G00098540 Rroxscaffold_2G00098560 Rroxscaffold_2G00098570 Rroxscaffold_2G00098650 Rroxscaffold_2G00098710 Rroxscaffold_2G00098740 Rroxscaffold_3G00225830 Rroxscaffold_3G00236870 Rroxscaffold_4G00312240
rosa_rugosa Rorug01G0047000 Rorug02G0372300 Rorug02G0407200 Rorug02G0407300 Rorug02G0408400 Rorug02G0421500 Rorug07G0208900
rosa_samantha Rh2AG380200 Rh2AG465700 Rh2AG465800 Rh2AG466800 Rh2AG467100 Rh2AG467600 Rh2AG480900 Rh2AG481400 Rh2AG481700 Rh2CG073500 Rh2CG452200 Rh2CG453200 Rh2CG453600 Rh2CG454100 Rh2CG467100 Rh2CG467800 Rh2CG468000 Rh2DG403100 Rh2DG487800 Rh2DG487900 Rh2DG488800 Rh2DG502000 Rh2DG502200 Rh2DG502300 Rh2DG503100 Rh3AG319300 Rh4AG227700 Rh4BG371800 Rh5AG327400 Rh5DG350600 Rh7AG349800
rosa_wichuraiana Rw0G010080 Rw2G031110 Rw2G037980 Rw2G037990 Rw2G038000 Rw2G038010 Rw2G038080 Rw2G038100 Rw2G039260 Rw2G039290 Rw2G039470 Rw4G019710 Rw5G030930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2135
Acc16I TGCGCA 2 cut(s) 563, 1931
Acc36I ACCTGC 1 cut(s) 1444
AccII CGCG 1 cut(s) 1667
AccIII TCCGGA 1 cut(s) 707
AciI CCGC 7 cut(s) 27, 199, 356, 1667, 2084, 2263, 2269
AclI AACGTT 1 cut(s) 1566
AclWI GGATC 7 cut(s) 89, 978, 1154, 1192, 1205, 1426, 2031
AcoI YGGCCR 2 cut(s) 592, 1750
AcsI RAATTY 1 cut(s) 456
AcuI CTGAAG 2 cut(s) 131, 1176
AfaI GTAC 4 cut(s) 918, 1553, 1867, 2049
AfeI AGCGCT 1 cut(s) 1547
AfiI CCNNNNNNNGG 7 cut(s) 104, 105, 587, 665, 2027, 2245, 2269
AflII CTTAAG 2 cut(s) 11, 1952
AjiI CACGTC 1 cut(s) 84
AjnI CCWGG 3 cut(s) 1607, 1716, 1747
Alw21I GWGCWC 2 cut(s) 447, 1703
Alw26I GTCTC 4 cut(s) 548, 1898, 2078, 2094
Alw44I GTGCAC 1 cut(s) 1699
AlwI GGATC 7 cut(s) 89, 978, 1154, 1192, 1205, 1426, 2031
AlwNI CAGNNNCTG 4 cut(s) 110, 211, 1625, 1898
Ama87I CYCGRG 1 cut(s) 437
Aor13HI TCCGGA 1 cut(s) 707
Aor51HI AGCGCT 1 cut(s) 1547
AoxI GGCC 6 cut(s) 350, 367, 592, 838, 1022, 1750
ApaLI GTGCAC 1 cut(s) 1699
ApeKI GCWGC 6 cut(s) 1625, 1892, 1895, 1922, 2123, 2219
ApoI RAATTY 1 cut(s) 456
ArsI GACNNNNNNTTYG 2 cut(s) 664, 696
Asp700I GAANNNNTTC 1 cut(s) 224
AspLEI GCGC 6 cut(s) 564, 1548, 1879, 1922, 1932, 1960
AspS9I GGNCC 7 cut(s) 310, 368, 578, 705, 839, 1023, 1319
AsuC2I CCSGG 1 cut(s) 1202
AsuHPI GGTGA 7 cut(s) 184, 197, 1099, 1394, 1538, 1774, 2171
AvaI CYCGRG 1 cut(s) 437
AvaII GGWCC 4 cut(s) 310, 578, 705, 1319
BaeGI GKGCMC 1 cut(s) 1703
BaeI ACNNNNGTAYC 2 cut(s) 677, 710
BalI TGGCCA 2 cut(s) 594, 1752
BamHI GGATCC 1 cut(s) 1197
BanII GRGCYC 1 cut(s) 2251
BbsI GAAGAC 2 cut(s) 137, 2182
Bbv12I GWGCWC 2 cut(s) 447, 1703
BbvCI CCTCAGC 1 cut(s) 126
BbvI GCAGC 6 cut(s) 1612, 1882, 1904, 1934, 2135, 2231
BciT130I CCWGG 3 cut(s) 1609, 1718, 1749
BciVI GTATCC 2 cut(s) 957, 1258
BclI TGATCA 1 cut(s) 1038
BcnI CCSGG 1 cut(s) 1202
BcoDI GTCTC 4 cut(s) 548, 1898, 2078, 2094
BfaI CTAG 2 cut(s) 1332, 1880
BfmI CTRYAG 4 cut(s) 288, 1470, 1580, 1626
BfoI RGCGCY 1 cut(s) 1549
BfrI CTTAAG 2 cut(s) 11, 1952
BfuAI ACCTGC 1 cut(s) 1444
BfuI GTATCC 2 cut(s) 957, 1258
BisI GCNGC 6 cut(s) 1626, 1893, 1896, 1923, 2124, 2220
BlsI GCNGC 6 cut(s) 1627, 1894, 1897, 1924, 2125, 2221
Bme1390I CCNGG 4 cut(s) 1202, 1609, 1718, 1749
Bme18I GGWCC 4 cut(s) 310, 578, 705, 1319
BmeT110I CYCGRG 1 cut(s) 437
BmgBI CACGTC 1 cut(s) 84
BmgT120I GGNCC 7 cut(s) 310, 368, 578, 705, 839, 1023, 1319
BmiI GGNNCC 4 cut(s) 1199, 1539, 1614, 2274
BmrFI CCNGG 4 cut(s) 1202, 1609, 1718, 1749
BmrI ACTGGG 1 cut(s) 174
BmsI GCATC 7 cut(s) 192, 283, 427, 772, 1195, 1227, 1497
BmuI ACTGGG 1 cut(s) 174
BpiI GAAGAC 2 cut(s) 137, 2182
BplI GAGNNNNNCTC 2 cut(s) 1688, 1720
BpmI CTGGAG 1 cut(s) 2205
Bpu10I CCTNAGC 2 cut(s) 126, 1542
BpuMI CCSGG 1 cut(s) 1202
BsaBI GATNNNNATC 1 cut(s) 1440
BsaI GGTCTC 3 cut(s) 548, 2078, 2094
BsaJI CCNNGG 7 cut(s) 438, 637, 933, 959, 1608, 1747, 2286
BsaWI WCCGGW 3 cut(s) 244, 447, 707
BsaXI ACNNNNNCTCC 4 cut(s) 226, 256, 957, 987
Bsc4I CCNNNNNNNGG 7 cut(s) 104, 105, 587, 665, 2027, 2245, 2269
Bse118I RCCGGY 1 cut(s) 365
Bse1I ACTGG 4 cut(s) 180, 911, 1273, 2188
Bse3DI GCAATG 3 cut(s) 1265, 2070, 2276
Bse8I GATNNNNATC 1 cut(s) 1440
BseAI TCCGGA 1 cut(s) 707
BseBI CCWGG 3 cut(s) 1609, 1718, 1749
BseDI CCNNGG 7 cut(s) 438, 637, 933, 959, 1608, 1747, 2286
BseGI GGATG 9 cut(s) 207, 763, 798, 955, 1121, 1231, 1236, 1454, 1828
BseJI GATNNNNATC 1 cut(s) 1440
BseLI CCNNNNNNNGG 7 cut(s) 104, 105, 587, 665, 2027, 2245, 2269
BseMI GCAATG 3 cut(s) 1265, 2070, 2276
BseNI ACTGG 4 cut(s) 180, 911, 1273, 2188
BseRI GAGGAG 2 cut(s) 378, 455
BseSI GKGCMC 1 cut(s) 1703
BseXI GCAGC 6 cut(s) 1612, 1882, 1904, 1934, 2135, 2231
BseYI CCCAGC 2 cut(s) 498, 2169
BsgI GTGCAG 1 cut(s) 2142
Bsh1236I CGCG 1 cut(s) 1667
BshFI GGCC 6 cut(s) 352, 369, 594, 840, 1024, 1752
BsiHKAI GWGCWC 2 cut(s) 447, 1703
BsiHKCI CYCGRG 1 cut(s) 437
BsiSI CCGG 6 cut(s) 245, 366, 448, 708, 1202, 1820
BslFI GGGAC 4 cut(s) 121, 516, 1184, 2100
BslI CCNNNNNNNGG 7 cut(s) 104, 105, 587, 665, 2027, 2245, 2269
BsmAI GTCTC 4 cut(s) 548, 1898, 2078, 2094
BsmFI GGGAC 4 cut(s) 121, 516, 1184, 2100
BsmI GAATGC 1 cut(s) 564
BsnI GGCC 6 cut(s) 352, 369, 594, 840, 1024, 1752
Bso31I GGTCTC 3 cut(s) 548, 2078, 2094
BsoBI CYCGRG 1 cut(s) 437
Bsp1286I GDGCHC 3 cut(s) 447, 1703, 2251
Bsp13I TCCGGA 1 cut(s) 707
Bsp19I CCATGG 1 cut(s) 637
BspACI CCGC 7 cut(s) 27, 199, 356, 1667, 2084, 2263, 2269
BspANI GGCC 6 cut(s) 352, 369, 594, 840, 1024, 1752
BspEI TCCGGA 1 cut(s) 707
BspFNI CGCG 1 cut(s) 1667
BspHI TCATGA 1 cut(s) 2193
BspLI GGNNCC 4 cut(s) 1199, 1539, 1614, 2274
BspMAI CTGCAG 2 cut(s) 1474, 1630
BspMI ACCTGC 1 cut(s) 1444
BspPI GGATC 7 cut(s) 89, 978, 1154, 1192, 1205, 1426, 2031
BspQI GCTCTTC 2 cut(s) 111, 1059
BspTI CTTAAG 2 cut(s) 11, 1952
BspTNI GGTCTC 3 cut(s) 548, 2078, 2094
BsrDI GCAATG 3 cut(s) 1265, 2070, 2276
BsrFI RCCGGY 1 cut(s) 365
BsrI ACTGG 4 cut(s) 180, 911, 1273, 2188
BssAI RCCGGY 1 cut(s) 365
BssECI CCNNGG 7 cut(s) 438, 637, 933, 959, 1608, 1747, 2286
BssT1I CCWWGG 4 cut(s) 637, 933, 959, 2286
Bst2UI CCWGG 3 cut(s) 1609, 1718, 1749
Bst4CI ACNGT 4 cut(s) 428, 1403, 1644, 1973
Bst6I CTCTTC 5 cut(s) 111, 129, 1014, 1059, 2118
BstAFI CTTAAG 2 cut(s) 11, 1952
BstAPI GCANNNNNTGC 1 cut(s) 1625
BstDSI CCRYGG 1 cut(s) 637
BstEII GGTNACC 1 cut(s) 185
BstENI CCTNNNNNAGG 1 cut(s) 2025
BstF5I GGATG 9 cut(s) 207, 763, 798, 955, 1121, 1231, 1236, 1454, 1828
BstFNI CGCG 1 cut(s) 1667
BstH2I RGCGCY 1 cut(s) 1549
BstHHI GCGC 6 cut(s) 564, 1548, 1879, 1922, 1932, 1960
BstMAI GTCTC 4 cut(s) 548, 1898, 2078, 2094
BstMWI GCNNNNNNNGC 8 cut(s) 332, 362, 555, 1151, 1292, 1625, 1718, 2090
BstNI CCWGG 3 cut(s) 1609, 1718, 1749
BstNSI RCATGY 5 cut(s) 562, 658, 805, 1399, 2209
BstPI GGTNACC 1 cut(s) 185
BstSCI CCNGG 4 cut(s) 1200, 1607, 1716, 1747
BstSFI CTRYAG 4 cut(s) 288, 1470, 1580, 1626
BstSLI GKGCMC 1 cut(s) 1703
BstUI CGCG 1 cut(s) 1667
BstV1I GCAGC 6 cut(s) 1612, 1882, 1904, 1934, 2135, 2231
BstV2I GAAGAC 2 cut(s) 137, 2182
BstX2I RGATCY 3 cut(s) 970, 1159, 1197
BstXI CCANNNNNNTGG 1 cut(s) 735
BstYI RGATCY 3 cut(s) 970, 1159, 1197
BsuI GTATCC 2 cut(s) 957, 1258
BsuRI GGCC 6 cut(s) 352, 369, 594, 840, 1024, 1752
BtgI CCRYGG 1 cut(s) 637
BtgZI GCGATG 1 cut(s) 354
BtrI CACGTC 1 cut(s) 84
BtsCI GGATG 9 cut(s) 207, 763, 798, 955, 1121, 1231, 1236, 1454, 1828
BtsI GCAGTG 1 cut(s) 1467
BtsIMutI CAGTG 5 cut(s) 187, 296, 433, 1467, 2181
BveI ACCTGC 1 cut(s) 1444
CaiI CAGNNNCTG 4 cut(s) 110, 211, 1625, 1898
CciI TCATGA 1 cut(s) 2193
CfoI GCGC 6 cut(s) 564, 1548, 1879, 1922, 1932, 1960
Cfr10I RCCGGY 1 cut(s) 365
Cfr13I GGNCC 7 cut(s) 310, 368, 578, 705, 839, 1023, 1319
CseI GACGC 1 cut(s) 110
Csp6I GTAC 4 cut(s) 917, 1552, 1866, 2048
CspCI CAANNNNNGTGG 2 cut(s) 1576, 1611
CviQI GTAC 4 cut(s) 917, 1552, 1866, 2048
DraI TTTAAA 1 cut(s) 1482
EaeI YGGCCR 2 cut(s) 592, 1750
Eam1104I CTCTTC 5 cut(s) 111, 129, 1014, 1059, 2118
EarI CTCTTC 5 cut(s) 111, 129, 1014, 1059, 2118
EciI GGCGGA 1 cut(s) 214
Eco130I CCWWGG 4 cut(s) 637, 933, 959, 2286
Eco24I GRGCYC 1 cut(s) 2251
Eco31I GGTCTC 3 cut(s) 548, 2078, 2094
Eco32I GATATC 1 cut(s) 943
Eco47I GGWCC 4 cut(s) 310, 578, 705, 1319
Eco47III AGCGCT 1 cut(s) 1547
Eco57I CTGAAG 2 cut(s) 131, 1176
Eco88I CYCGRG 1 cut(s) 437
Eco91I GGTNACC 1 cut(s) 185
EcoNI CCTNNNNNAGG 1 cut(s) 2025
EcoO109I RGGNCCY 1 cut(s) 578
EcoO65I GGTNACC 1 cut(s) 185
EcoRII CCWGG 3 cut(s) 1607, 1716, 1747
EcoRV GATATC 1 cut(s) 943
EcoT14I CCWWGG 4 cut(s) 637, 933, 959, 2286
EcoT22I ATGCAT 2 cut(s) 765, 1397
EcoT38I GRGCYC 1 cut(s) 2251
ErhI CCWWGG 4 cut(s) 637, 933, 959, 2286
FalI AAGNNNNNCTT 2 cut(s) 1275, 1307
FaqI GGGAC 4 cut(s) 121, 516, 1184, 2100
FauI CCCGC 3 cut(s) 20, 2077, 2262
FauNDI CATATG 2 cut(s) 398, 1861
FbaI TGATCA 1 cut(s) 1038
Fnu4HI GCNGC 6 cut(s) 1626, 1893, 1896, 1923, 2124, 2220
FokI GGATG 9 cut(s) 214, 750, 805, 942, 1128, 1238, 1243, 1461, 1835
FriOI GRGCYC 1 cut(s) 2251
Fsp4HI GCNGC 6 cut(s) 1626, 1893, 1896, 1923, 2124, 2220
FspAI RTGCGCAY 1 cut(s) 563
FspBI CTAG 2 cut(s) 1332, 1880
FspI TGCGCA 2 cut(s) 563, 1931
GlaI GCGC 6 cut(s) 563, 1547, 1878, 1921, 1931, 1959
GluI GCNGC 6 cut(s) 1626, 1893, 1896, 1923, 2124, 2220
GsaI CCCAGC 2 cut(s) 502, 2173
GsuI CTGGAG 1 cut(s) 2205
HaeII RGCGCY 1 cut(s) 1549
HaeIII GGCC 6 cut(s) 352, 369, 594, 840, 1024, 1752
HapII CCGG 6 cut(s) 245, 366, 448, 708, 1202, 1820
HgaI GACGC 1 cut(s) 110
HhaI GCGC 6 cut(s) 564, 1548, 1879, 1922, 1932, 1960
Hin6I GCGC 6 cut(s) 562, 1546, 1877, 1920, 1930, 1958
HinP1I GCGC 6 cut(s) 562, 1546, 1877, 1920, 1930, 1958
HincII GTYRAC 1 cut(s) 1564
HindII GTYRAC 1 cut(s) 1564
HindIII AAGCTT 1 cut(s) 8
HinfI GANTC 5 cut(s) 35, 628, 739, 1556, 2187
HpaII CCGG 6 cut(s) 245, 366, 448, 708, 1202, 1820
HphI GGTGA 7 cut(s) 184, 197, 1099, 1394, 1538, 1774, 2171
Hpy166II GTNNAC 6 cut(s) 313, 799, 1425, 1564, 1701, 2003
Hpy188III TCNNGA 9 cut(s) 98, 211, 612, 632, 708, 1180, 1351, 1439, 2194
Hpy8I GTNNAC 6 cut(s) 313, 799, 1425, 1564, 1701, 2003
Hpy99I CGWCG 2 cut(s) 74, 104
HpyCH4III ACNGT 4 cut(s) 428, 1403, 1644, 1973
HpyCH4IV ACGT 2 cut(s) 83, 1566
HpyF10VI GCNNNNNNNGC 8 cut(s) 332, 362, 555, 1151, 1292, 1625, 1718, 2090
HpySE526I ACGT 2 cut(s) 83, 1566
HspAI GCGC 6 cut(s) 562, 1546, 1877, 1920, 1930, 1958
Kpn2I TCCGGA 1 cut(s) 707
KroI GCCGGC 1 cut(s) 365
KroNI GCCGGC 1 cut(s) 367
Ksp22I TGATCA 1 cut(s) 1038
LguI GCTCTTC 2 cut(s) 111, 1059
LmnI GCTCC 2 cut(s) 442, 1612
Lsp1109I GCAGC 6 cut(s) 1612, 1882, 1904, 1934, 2135, 2231
LweI GCATC 7 cut(s) 192, 283, 427, 772, 1195, 1227, 1497
MaeI CTAG 2 cut(s) 1332, 1880
MaeII ACGT 2 cut(s) 83, 1566
MaeIII GTNAC 7 cut(s) 185, 464, 518, 646, 1060, 1382, 1403
MflI RGATCY 3 cut(s) 970, 1159, 1197
MhlI GDGCHC 3 cut(s) 447, 1703, 2251
MlsI TGGCCA 2 cut(s) 594, 1752
MluCI AATT 6 cut(s) 237, 252, 456, 843, 1840, 2140
MluNI TGGCCA 2 cut(s) 594, 1752
MlyI GAGTC 1 cut(s) 2196
MmeI TCCRAC 3 cut(s) 52, 1676, 2064
Mox20I TGGCCA 2 cut(s) 594, 1752
Mph1103I ATGCAT 2 cut(s) 765, 1397
MroI TCCGGA 1 cut(s) 707
MroNI GCCGGC 1 cut(s) 365
MroXI GAANNNNTTC 1 cut(s) 224
MscI TGGCCA 2 cut(s) 594, 1752
MseI TTAA 5 cut(s) 12, 1481, 1646, 1734, 1953
MslI CAYNNNNRTG 8 cut(s) 401, 483, 557, 642, 653, 806, 927, 1689
Msp20I TGGCCA 2 cut(s) 594, 1752
MspA1I CMGCKG 2 cut(s) 1895, 2149
MspCI CTTAAG 2 cut(s) 11, 1952
MspI CCGG 6 cut(s) 245, 366, 448, 708, 1202, 1820
MspR9I CCNGG 4 cut(s) 1202, 1609, 1718, 1749
Mva1269I GAATGC 1 cut(s) 564
MvaI CCWGG 3 cut(s) 1609, 1718, 1749
MvnI CGCG 1 cut(s) 1667
MwoI GCNNNNNNNGC 8 cut(s) 332, 362, 555, 1151, 1292, 1625, 1718, 2090
NaeI GCCGGC 1 cut(s) 367
NciI CCSGG 1 cut(s) 1202
NcoI CCATGG 1 cut(s) 637
NdeI CATATG 2 cut(s) 398, 1861
NgoMIV GCCGGC 1 cut(s) 365
NlaIV GGNNCC 4 cut(s) 1199, 1539, 1614, 2274
NmuCI GTSAC 6 cut(s) 185, 518, 646, 1060, 1382, 1403
NsbI TGCGCA 2 cut(s) 563, 1931
NsiI ATGCAT 2 cut(s) 765, 1397
NspI RCATGY 5 cut(s) 562, 658, 805, 1399, 2209
PaeI GCATGC 3 cut(s) 562, 1399, 2209
PagI TCATGA 1 cut(s) 2193
PciSI GCTCTTC 2 cut(s) 111, 1059
PctI GAATGC 1 cut(s) 564
PdiI GCCGGC 1 cut(s) 367
PdmI GAANNNNTTC 1 cut(s) 224
PfeI GAWTC 4 cut(s) 35, 628, 739, 1556
PfoI TCCNGGA 1 cut(s) 1200
PkrI GCNGC 6 cut(s) 1627, 1894, 1897, 1924, 2125, 2221
PleI GAGTC 1 cut(s) 2195
PpsI GAGTC 1 cut(s) 2195
PpuMI RGGWCCY 1 cut(s) 578
PsiI TTATAA 1 cut(s) 2135
Psp1406I AACGTT 1 cut(s) 1566
Psp5II RGGWCCY 1 cut(s) 578
Psp6I CCWGG 3 cut(s) 1607, 1716, 1747
PspEI GGTNACC 1 cut(s) 185
PspFI CCCAGC 2 cut(s) 498, 2169
PspGI CCWGG 3 cut(s) 1607, 1716, 1747
PspN4I GGNNCC 4 cut(s) 1199, 1539, 1614, 2274
PspPI GGNCC 7 cut(s) 310, 368, 578, 705, 839, 1023, 1319
PspPPI RGGWCCY 1 cut(s) 578
PsrI GAACNNNNNNTAC 2 cut(s) 1085, 1117
PstI CTGCAG 2 cut(s) 1474, 1630
PstNI CAGNNNCTG 4 cut(s) 110, 211, 1625, 1898
PsuI RGATCY 3 cut(s) 970, 1159, 1197
PvuII CAGCTG 2 cut(s) 1895, 2149
RsaI GTAC 4 cut(s) 918, 1553, 1867, 2049
RsaNI GTAC 4 cut(s) 917, 1552, 1866, 2048
RseI CAYNNNNRTG 8 cut(s) 401, 483, 557, 642, 653, 806, 927, 1689
SapI GCTCTTC 2 cut(s) 111, 1059
SaqAI TTAA 5 cut(s) 12, 1481, 1646, 1734, 1953
SatI GCNGC 6 cut(s) 1626, 1893, 1896, 1923, 2124, 2220
Sau96I GGNCC 7 cut(s) 310, 368, 578, 705, 839, 1023, 1319
SchI GAGTC 1 cut(s) 2196
ScrFI CCNGG 4 cut(s) 1202, 1609, 1718, 1749
SduI GDGCHC 3 cut(s) 447, 1703, 2251
SfaNI GCATC 7 cut(s) 192, 283, 427, 772, 1195, 1227, 1497
SfcI CTRYAG 4 cut(s) 288, 1470, 1580, 1626
SinI GGWCC 4 cut(s) 310, 578, 705, 1319
SmiMI CAYNNNNRTG 8 cut(s) 401, 483, 557, 642, 653, 806, 927, 1689
SmlI CTYRAG 2 cut(s) 11, 1952
SmoI CTYRAG 2 cut(s) 11, 1952
SphI GCATGC 3 cut(s) 562, 1399, 2209
Sse9I AATT 6 cut(s) 237, 252, 456, 843, 1840, 2140
SsiI CCGC 7 cut(s) 27, 199, 356, 1667, 2084, 2263, 2269
SspMI CTAG 2 cut(s) 1332, 1880
StyD4I CCNGG 4 cut(s) 1200, 1607, 1716, 1747
StyI CCWWGG 4 cut(s) 637, 933, 959, 2286
TaaI ACNGT 4 cut(s) 428, 1403, 1644, 1973
TaiI ACGT 2 cut(s) 86, 1569
TaqI TCGA 5 cut(s) 93, 513, 683, 1502, 1770
TaqII GACCGA 1 cut(s) 298
TasI AATT 6 cut(s) 237, 252, 456, 843, 1840, 2140
TatI WGTACW 1 cut(s) 2047
TfiI GAWTC 4 cut(s) 35, 628, 739, 1556
Tru1I TTAA 5 cut(s) 12, 1481, 1646, 1734, 1953
Tru9I TTAA 5 cut(s) 12, 1481, 1646, 1734, 1953
TscAI CASTG 5 cut(s) 187, 303, 433, 1474, 2188
TseFI GTSAC 6 cut(s) 185, 518, 646, 1060, 1382, 1403
TseI GCWGC 6 cut(s) 1625, 1892, 1895, 1922, 2123, 2219
Tsp45I GTSAC 6 cut(s) 185, 518, 646, 1060, 1382, 1403
TspGWI ACGGA 1 cut(s) 1322
TspRI CASTG 5 cut(s) 187, 303, 433, 1474, 2188
Vha464I CTTAAG 2 cut(s) 11, 1952
VneI GTGCAC 1 cut(s) 1699
VpaK11BI GGWCC 4 cut(s) 310, 578, 705, 1319
XagI CCTNNNNNAGG 1 cut(s) 2025
XapI RAATTY 1 cut(s) 456
XceI RCATGY 5 cut(s) 562, 658, 805, 1399, 2209
XcmI CCANNNNNNNNNTGG 2 cut(s) 774, 1117
XmnI GAANNNNTTC 1 cut(s) 224
XspI CTAG 2 cut(s) 1332, 1880
Zsp2I ATGCAT 2 cut(s) 765, 1397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.