RchiOBHm_Chr2g0148981

Belongs to the terpene cyclase mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000173
Physical Location & Seq
Forward (+)
66822152 .. 66827404
5253 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51841

Sequence Viewer

Length: 2280 bp
ATGTGGAGGATTAAGTTCGGAGAGGGTGACAATGACCCTTTGCTCTTCAGCACAAACAACTTCCACGGGAGGCAGACATGGGAGTTCGACCCTGATGCAGGCACACCGGAAGAGCGCGCCGAGGTTGAGGCCGCTCGTGAGAATTTCTACCAGAATCGCTACAAGGTTGCTCCCAGCAGTGACCTCCTCTGGCGTTTTCAGATGCTAAGAGAGAGAAACTTCAAGCAAGAAATTCCTCCGGTGAGAATTGGGGAAGGTGAGGAAATCACATTTGATCAGGCCACAGCTGCATACAAGAGGGCTGCTTCCTTCTGGAATGCCTTGCAGTCACCCCATGGTCACTGGCCTGCTGAGAATGCTGGCCCAAACTTTTACTTTCCTCCATTGGTCATGAGTGTATACATACCAGGATACCTCAATGTTGTTTTCCCTGCCGAGTACAAGAAGGAAATCTTGCGCTACACATACAACCATCAGAACGAAGATGGTGGCTGGGGTTTGCACATTGCAGGGCCTAGTATGATGTTCACCACATGTCTCAACTATTGTATGATGCGTATTCTCGGAGAAGGCCCTGAGGGTGGTCGAGACAACGCCTGTGCAAGGGCCCGGAAATGGATTCTTGACCGCGGTGGTGCACAATATTCTGCCTCATGGGGAAAGACCTGGATGGCGATACTTGGTGTTTATGATTGGGAAGGAAGCAACCCAATGCCCCCAGAGTTTTGGACCTACCCTACTCTGCTTCCATTTCATCCATCAAAAATGTTTTGCTACTGTAGGTTGACTTACCTACCCATGTCCTACTTTTATGCAACAAGATTTGTTGGCCCAATCACTCCTCTTGTTGAGCAACTGAGACAAGAAATCTACAATGAGCCTTACGATCAAATCAAGTGGAGTCAAGTGCGCCATCATTGTGCAACAGAAGATAACTACTATCCTCATGGAAGAGTACAGCGCTTTATGTGGGATAGTCTTTACAATGTTTGTGAGCCTCTTCTCAAAATCTGGCCCTTCAAAAAGATCAGAGACAATGCTGTTCAATTCACCATTGACCAAATTCATTACGAAGACGAGAACAGTCGCTACATTACCATTGGATGTGTTGAAAAGCCATTGATGATGCTTGCTTGTTGGGCTGAGGATCCTAGTGGAGAAGCTTTTAAGAAGCATCTTCCTAGAGTCACTGATTATATTTGGCTCGGTGAAGATGGTATCAAGATGCAGAGTTTCGGTAGTCAGTCATGGGATTGTGCTCTTGTGATTCAAGCATTGCTTTGTGGGAATCTCAACAATGAATTTGGACCTGTACTTAAGCTGGCACATGACTTCCTAAAGAAATCTCAGGTGAGGATGAATACTTCCGGCGACTACTTGGCTCATTTCCGTCATATTTCTAAAGGATGCTGGACTTTCTCTGATCGGGATCATGGATGGCAAGTGTCTGACTGCACTGCAGAAGCATTGAGGTGCTGCTGTCTGTTTGCAATGATGTCACCAGAGCTTGTTGGTGAGCCAATGGAAGCTGAGTGTATGTATGATGCTGTCAATGTCATAATGTCACTACAGAGTCCAAATGGTGGTGTATCGGCCTGGGAGCCAACTGGAGCTCCTAAATGGTTGGAGTGGCTCAACCCCGTCGAATTTCTTGAGGACCTTGTCATTGAATACGAATACATCGAGTGTACTTCATCTTCAATCCAGGCCCTAGTGTTGTTTAGGAAGTTGTACCCTGGTCACAGAAGGAAGGAGATTAACAACTTCATCACAAGGGCTGCAGACTACATTGAAAGTATCCAATACCCTGATGGTTCATGGTATGGAAACTGGGGTATCTGCTTTGTCTATGGAACATGGTTCGGAATCAAAGGATTAGAGGCTGCTGGAAGAACATACAACAACTGTGAGGCTGTTCGTAAAGGTGTCGATTTCTTGCTCAATTCACAGAGGGAAGATGGTGGTTGGGGAGAGCACTACACCTCATGCACAAATAAGAAATACACAGCTCAGGACAGTACAAATCTGGTGCAAACTGCCCTTGGATTGATGGGTCTAATTCACGGTCGACAGGCTGAGAGAGATCCAACTCCTATCCACCGTGCCGCAAGGGTGTTGATGAATGGTCAATTGGATGACGGTGATTTCCCCCAACAGGAACTGATGGGAGTTTTCATGAGGAATGCCATGTTGCACTATGCAGCATATAGAAATATCTTCCCATTGTGGGCTCTCGGAGAGTACCGCACTCTGGTGCAATTGCCTACCAAAAGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 ko01110 map00909 map01110
Pfam Domains
Protein Families
SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

759

Amino Acids

87.64

Weight (kDa)

5.74

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQHop_cyclase_N PF13249 144 - 396 1.2e-32 Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C PF13243 416 - 749 7.5e-48 Squalene-hopene cyclase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66960 AT1G66960 AT1G66960 AT1G66960 AT1G78950 AT1G78950 AT1G78955 AT1G78955 AT1G78955 AT1G78955 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78970 AT1G78970 AT1G78970
fragaria_vesca FvH4_5g29480 FvH4_6g36450 FvH4_6g36450 FvH4_6g36500 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g37620
malus_domestica MD03G1089600.v1.1 MD09G1167700.v1.1 MD09G1168200.v1.1 MD09G1168300.v1.1 MD11G1098600.v1.1 MD17G1158300.v1.1 MD17G1158700.v1.1 MD17G1159500.v1.1 MD17G1160500.v1.1 MD17G1182500.v1.1 MD17G1182700.v1.1 MD17G1245800.v1.1 MD17G1246300.v1.1 MD17G1246600.v1.1
prunus_persica Prupe.3G025700_v2.0.a1 Prupe.3G025700_v2.0.a1 Prupe.3G025800_v2.0.a1 Prupe.3G025900_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026400_v2.0.a1 Prupe.3G026500_v2.0.a1 Prupe.3G026700_v2.0.a1 Prupe.3G026800_v2.0.a1
pyrus_communis pycom03g07120 pycom09g08520 pycom09g08590 pycom09g08610 pycom10g10070 pycom11g08310 pycom16g19880 pycom17g15190 pycom17g15300
rosa_chinensis RchiOBHm_Chr2g0136221 RchiOBHm_Chr2g0148451 RchiOBHm_Chr2g0148501 RchiOBHm_Chr2g0148521 RchiOBHm_Chr2g0148661 RchiOBHm_Chr2g0148701 RchiOBHm_Chr2g0148811 RchiOBHm_Chr2g0148941 RchiOBHm_Chr2g0148981 RchiOBHm_Chr2g0150451 RchiOBHm_Chr2g0150521 RchiOBHm_Chr2g0150611 RchiOBHm_Chr4g0420831 RchiOBHm_Chr5g0049791 RchiOBHm_Chr5g0049801
rosa_laevigata RLG00000007735 RLG00000020351 RLG00000020352 RLG00000020357 RLG00000020358 RLG00000020360 RLG00000029093 RLG00000030686
rosa_multiflora Rmu_co8274513.1_g000001 Rmu_sc0000048.1_g000003 Rmu_sc0000048.1_g000033 Rmu_sc0000048.1_g000039 Rmu_sc0001397.1_g000011 Rmu_sc0001998.1_g000039 Rmu_sc0002070.1_g000055 Rmu_sc0002082.1_g000052 Rmu_sc0003181.1_g000010 Rmu_sc0005104.1_g000005 Rmu_sc0006758.1_g000002 Rmu_sc0008411.1_g000029 Rmu_sc0008411.1_g000030 Rmu_sc0017472.1_g000001 Rmu_sc0017472.1_g000003 Rmu_sc0029876.1_g000001 Rmu_ssc0000480.1_g000024 Rmu_ssc0000480.1_g000036
rosa_roxburghii Rroxscaffold_2G00097070 Rroxscaffold_2G00098540 Rroxscaffold_2G00098560 Rroxscaffold_2G00098570 Rroxscaffold_2G00098650 Rroxscaffold_2G00098710 Rroxscaffold_2G00098740 Rroxscaffold_3G00225830 Rroxscaffold_3G00236870 Rroxscaffold_4G00312240
rosa_rugosa Rorug01G0047000 Rorug02G0372300 Rorug02G0407200 Rorug02G0407300 Rorug02G0408400 Rorug02G0421500 Rorug07G0208900
rosa_samantha Rh2AG380200 Rh2AG465700 Rh2AG465800 Rh2AG466800 Rh2AG467100 Rh2AG467600 Rh2AG480900 Rh2AG481400 Rh2AG481700 Rh2CG073500 Rh2CG452200 Rh2CG453200 Rh2CG453600 Rh2CG454100 Rh2CG467100 Rh2CG467800 Rh2CG468000 Rh2DG403100 Rh2DG487800 Rh2DG487900 Rh2DG488800 Rh2DG502000 Rh2DG502200 Rh2DG502300 Rh2DG503100 Rh3AG319300 Rh4AG227700 Rh4BG371800 Rh5AG327400 Rh5DG350600 Rh7AG349800
rosa_wichuraiana Rw0G010080 Rw2G031110 Rw2G037980 Rw2G037990 Rw2G038000 Rw2G038010 Rw2G038080 Rw2G038100 Rw2G039260 Rw2G039290 Rw2G039470 Rw4G019710 Rw5G030930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1583
AccBSI CCGCTC 1 cut(s) 134
AccI GTMKAC 2 cut(s) 399, 2068
AccII CGCG 2 cut(s) 117, 630
AciI CCGC 5 cut(s) 132, 628, 630, 2106, 2245
AclWI GGATC 4 cut(s) 1142, 1155, 1437, 2078
AcsI RAATTY 5 cut(s) 142, 231, 1062, 1301, 1646
AcuI CTGAAG 1 cut(s) 31
AfaI GTAC 7 cut(s) 440, 957, 1314, 1690, 1733, 2020, 2243
AfeI AGCGCT 1 cut(s) 962
AfiI CCNNNNNNNGG 8 cut(s) 98, 581, 603, 615, 1583, 2155, 2227, 2251
AflII CTTAAG 1 cut(s) 1316
AflIII ACRYGT 1 cut(s) 533
AgsI TTSAA 8 cut(s) 223, 1021, 1046, 1112, 1271, 1670, 1701, 1793
AjnI CCWGG 5 cut(s) 406, 665, 1595, 1704, 1735
AjuI GAANNNNNNNTTGG 2 cut(s) 2114, 2146
AleI CACNNNNGTG 1 cut(s) 2252
AluBI AGCT 7 cut(s) 287, 1163, 1321, 1507, 1529, 1613, 2009
AluI AGCT 7 cut(s) 287, 1163, 1321, 1507, 1529, 1613, 2009
Alw21I GWGCWC 4 cut(s) 640, 1261, 1615, 1977
Alw26I GTCTC 4 cut(s) 542, 582, 853, 1026
Alw44I GTGCAC 1 cut(s) 636
AlwI GGATC 4 cut(s) 1142, 1155, 1437, 2078
AlwNI CAGNNNCTG 1 cut(s) 2161
Aor51HI AGCGCT 1 cut(s) 962
ApaI GGGCCC 1 cut(s) 610
ApaLI GTGCAC 1 cut(s) 636
ApeKI GCWGC 6 cut(s) 287, 302, 1476, 1778, 1883, 2201
ApoI RAATTY 5 cut(s) 142, 231, 1062, 1301, 1646
AspLEI GCGC 5 cut(s) 117, 119, 459, 912, 963
AsuC2I CCSGG 1 cut(s) 610
AvaII GGWCC 3 cut(s) 729, 1307, 1657
AxyI CCTNAGG 1 cut(s) 576
BaeGI GKGCMC 2 cut(s) 610, 640
BaeI ACNNNNGTAYC 2 cut(s) 1819, 1852
BamHI GGATCC 1 cut(s) 1147
BanII GRGCYC 3 cut(s) 610, 1615, 2233
BarI GAAGNNNNNNTAC 2 cut(s) 1681, 1713
BauI CACGAG 1 cut(s) 135
BbsI GAAGAC 1 cut(s) 1080
Bbv12I GWGCWC 4 cut(s) 640, 1261, 1615, 1977
BbvCI CCTCAGC 1 cut(s) 1143
BbvI GCAGC 6 cut(s) 274, 289, 1463, 1765, 1870, 2213
BcgI CGANNNNNNTGC 2 cut(s) 77, 111
BciT130I CCWGG 5 cut(s) 408, 667, 1597, 1706, 1737
BciVI GTATCC 2 cut(s) 404, 1808
BclI TGATCA 1 cut(s) 274
BcnI CCSGG 1 cut(s) 610
BcoDI GTCTC 4 cut(s) 542, 582, 853, 1026
BfaI CTAG 4 cut(s) 516, 1152, 1182, 1712
BfmI CTRYAG 4 cut(s) 778, 1458, 1568, 1779
BfoI RGCGCY 1 cut(s) 964
BfrI CTTAAG 1 cut(s) 1316
BfuI GTATCC 2 cut(s) 404, 1808
BisI GCNGC 8 cut(s) 132, 288, 303, 1477, 1779, 1884, 2106, 2202
BlsI GCNGC 8 cut(s) 133, 289, 304, 1478, 1780, 1885, 2107, 2203
Bme1390I CCNGG 6 cut(s) 408, 610, 667, 1597, 1706, 1737
Bme18I GGWCC 3 cut(s) 729, 1307, 1657
BmiI GGNNCC 3 cut(s) 608, 1149, 1602
BmrFI CCNGG 6 cut(s) 408, 610, 667, 1597, 1706, 1737
BmrI ACTGGG 1 cut(s) 1840
BmsI GCATC 8 cut(s) 85, 192, 543, 1116, 1183, 1215, 1397, 1534
BmuI ACTGGG 1 cut(s) 1840
BpiI GAAGAC 1 cut(s) 1080
BpmI CTGGAG 1 cut(s) 1629
Bpu10I CCTNAGC 2 cut(s) 1143, 2010
BpuEI CTTGAG 1 cut(s) 1673
BpuMI CCSGG 1 cut(s) 610
BsaBI GATNNNNATC 1 cut(s) 1428
BsaJI CCNNGG 7 cut(s) 64, 120, 334, 628, 1596, 1735, 2041
BsaWI WCCGGW 2 cut(s) 106, 238
BsaXI ACNNNNNCTCC 2 cut(s) 1597, 1627
Bsc4I CCNNNNNNNGG 8 cut(s) 98, 581, 603, 615, 1583, 2155, 2227, 2251
Bse1I ACTGG 3 cut(s) 347, 1612, 1835
Bse21I CCTNAGG 1 cut(s) 576
Bse3DI GCAATG 3 cut(s) 504, 1274, 1497
Bse8I GATNNNNATC 1 cut(s) 1428
BseBI CCWGG 5 cut(s) 408, 667, 1597, 1706, 1737
BseDI CCNNGG 7 cut(s) 64, 120, 334, 628, 1596, 1735, 2041
BseGI GGATG 7 cut(s) 675, 754, 1109, 1362, 1412, 1442, 2140
BseJI GATNNNNATC 1 cut(s) 1428
BseLI CCNNNNNNNGG 8 cut(s) 98, 581, 603, 615, 1583, 2155, 2227, 2251
BseMI GCAATG 3 cut(s) 504, 1274, 1497
BseMII CTCAG 8 cut(s) 342, 567, 848, 1134, 1361, 1521, 2024, 2067
BseNI ACTGG 3 cut(s) 347, 1612, 1835
BsePI GCGCGC 1 cut(s) 115
BseRI GAGGAG 2 cut(s) 176, 831
BseSI GKGCMC 2 cut(s) 610, 640
BseXI GCAGC 6 cut(s) 274, 289, 1463, 1765, 1870, 2213
BseYI CCCAGC 2 cut(s) 173, 492
BsgI GTGCAG 1 cut(s) 1438
Bsh1236I CGCG 2 cut(s) 117, 630
Bsh1285I CGRYCG 1 cut(s) 2068
BsiEI CGRYCG 1 cut(s) 2068
BsiHKAI GWGCWC 4 cut(s) 640, 1261, 1615, 1977
BsiSI CCGG 4 cut(s) 107, 239, 610, 1368
BslI CCNNNNNNNGG 8 cut(s) 98, 581, 603, 615, 1583, 2155, 2227, 2251
BsmAI GTCTC 4 cut(s) 542, 582, 853, 1026
BsmI GAATGC 3 cut(s) 322, 361, 2188
Bsp120I GGGCCC 1 cut(s) 606
Bsp1286I GDGCHC 6 cut(s) 610, 640, 1261, 1615, 1977, 2233
Bsp143I GATC 7 cut(s) 274, 886, 1026, 1147, 1423, 1429, 2083
Bsp19I CCATGG 1 cut(s) 334
BspACI CCGC 5 cut(s) 132, 628, 630, 2106, 2245
BspCNI CTCAG 8 cut(s) 343, 568, 849, 1135, 1360, 1522, 2023, 2068
BspFNI CGCG 2 cut(s) 117, 630
BspHI TCATGA 2 cut(s) 390, 2175
BspLI GGNNCC 3 cut(s) 608, 1149, 1602
BspMAI CTGCAG 2 cut(s) 1462, 1783
BspPI GGATC 4 cut(s) 1142, 1155, 1437, 2078
BspQI GCTCTTC 2 cut(s) 50, 105
BspTI CTTAAG 1 cut(s) 1316
BsrBI CCGCTC 1 cut(s) 134
BsrDI GCAATG 3 cut(s) 504, 1274, 1497
BsrI ACTGG 3 cut(s) 347, 1612, 1835
BssECI CCNNGG 7 cut(s) 64, 120, 334, 628, 1596, 1735, 2041
BssHII GCGCGC 1 cut(s) 115
BssMI GATC 7 cut(s) 274, 886, 1026, 1147, 1423, 1429, 2083
BssNAI GTATAC 1 cut(s) 400
BssSI CACGAG 1 cut(s) 135
BssT1I CCWWGG 2 cut(s) 334, 2041
Bst1107I GTATAC 1 cut(s) 400
Bst2BI CACGAG 1 cut(s) 135
Bst2UI CCWGG 5 cut(s) 408, 667, 1597, 1706, 1737
Bst4CI ACNGT 7 cut(s) 779, 1085, 1907, 2018, 2066, 2102, 2141
Bst6I CTCTTC 4 cut(s) 50, 105, 946, 1005
BstAFI CTTAAG 1 cut(s) 1316
BstC8I GCNNGC 6 cut(s) 100, 117, 348, 361, 1131, 1323
BstDEI CTNAG 9 cut(s) 206, 351, 576, 857, 1143, 1347, 1530, 2010, 2076
BstDSI CCRYGG 3 cut(s) 64, 334, 628
BstENI CCTNNNNNAGG 2 cut(s) 96, 601
BstF5I GGATG 7 cut(s) 675, 754, 1109, 1362, 1412, 1442, 2140
BstFNI CGCG 2 cut(s) 117, 630
BstH2I RGCGCY 1 cut(s) 964
BstHHI GCGC 5 cut(s) 117, 119, 459, 912, 963
BstKTI GATC 7 cut(s) 277, 889, 1029, 1150, 1426, 1432, 2086
BstMAI GTCTC 4 cut(s) 542, 582, 853, 1026
BstMBI GATC 7 cut(s) 274, 886, 1026, 1147, 1423, 1429, 2083
BstMCI CGRYCG 1 cut(s) 2068
BstMWI GCNNNNNNNGC 3 cut(s) 287, 356, 1139
BstNI CCWGG 5 cut(s) 408, 667, 1597, 1706, 1737
BstNSI RCATGY 1 cut(s) 537
BstSCI CCNGG 6 cut(s) 406, 608, 665, 1595, 1704, 1735
BstSFI CTRYAG 4 cut(s) 778, 1458, 1568, 1779
BstSLI GKGCMC 2 cut(s) 610, 640
BstUI CGCG 2 cut(s) 117, 630
BstV1I GCAGC 6 cut(s) 274, 289, 1463, 1765, 1870, 2213
BstV2I GAAGAC 1 cut(s) 1080
BstX2I RGATCY 2 cut(s) 1147, 2083
BstXI CCANNNNNNTGG 1 cut(s) 726
BstYI RGATCY 2 cut(s) 1147, 2083
BstZ17I GTATAC 1 cut(s) 400
Bsu36I CCTNAGG 1 cut(s) 576
BsuI GTATCC 2 cut(s) 404, 1808
BtgI CCRYGG 3 cut(s) 64, 334, 628
BtsCI GGATG 7 cut(s) 675, 754, 1109, 1362, 1412, 1442, 2140
BtsI GCAGTG 2 cut(s) 184, 1455
BtsIMutI CAGTG 4 cut(s) 184, 340, 1188, 1455
Cac8I GCNNGC 6 cut(s) 100, 117, 348, 361, 1131, 1323
CaiI CAGNNNCTG 1 cut(s) 2161
CciI TCATGA 2 cut(s) 390, 2175
CfoI GCGC 5 cut(s) 117, 119, 459, 912, 963
Cfr42I CCGCGG 1 cut(s) 631
Csp6I GTAC 7 cut(s) 439, 956, 1313, 1689, 1732, 2019, 2242
CspCI CAANNNNNGTGG 2 cut(s) 878, 913
CviQI GTAC 7 cut(s) 439, 956, 1313, 1689, 1732, 2019, 2242
DdeI CTNAG 9 cut(s) 206, 351, 576, 857, 1143, 1347, 1530, 2010, 2076
DpnI GATC 7 cut(s) 276, 888, 1028, 1149, 1425, 1431, 2085
DpnII GATC 7 cut(s) 274, 886, 1026, 1147, 1423, 1429, 2083
Eam1104I CTCTTC 4 cut(s) 50, 105, 946, 1005
EarI CTCTTC 4 cut(s) 50, 105, 946, 1005
Ecl136II GAGCTC 1 cut(s) 1613
Eco130I CCWWGG 2 cut(s) 334, 2041
Eco24I GRGCYC 3 cut(s) 610, 1615, 2233
Eco47I GGWCC 3 cut(s) 729, 1307, 1657
Eco47III AGCGCT 1 cut(s) 962
Eco53kI GAGCTC 1 cut(s) 1613
Eco57I CTGAAG 1 cut(s) 31
Eco81I CCTNAGG 1 cut(s) 576
EcoICRI GAGCTC 1 cut(s) 1613
EcoNI CCTNNNNNAGG 2 cut(s) 96, 601
EcoO109I RGGNCCY 5 cut(s) 512, 572, 606, 1657, 1708
EcoRII CCWGG 5 cut(s) 406, 665, 1595, 1704, 1735
EcoT14I CCWWGG 2 cut(s) 334, 2041
EcoT38I GRGCYC 3 cut(s) 610, 1615, 2233
ErhI CCWWGG 2 cut(s) 334, 2041
FalI AAGNNNNNCTT 4 cut(s) 437, 469, 1263, 1295
FbaI TGATCA 1 cut(s) 274
FblI GTMKAC 2 cut(s) 399, 2068
Fnu4HI GCNGC 8 cut(s) 132, 288, 303, 1477, 1779, 1884, 2106, 2202
FokI GGATG 7 cut(s) 682, 741, 1116, 1369, 1419, 1449, 2147
FriOI GRGCYC 3 cut(s) 610, 1615, 2233
Fsp4HI GCNGC 8 cut(s) 132, 288, 303, 1477, 1779, 1884, 2106, 2202
FspBI CTAG 4 cut(s) 516, 1152, 1182, 1712
GlaI GCGC 5 cut(s) 116, 118, 458, 911, 962
GluI GCNGC 8 cut(s) 132, 288, 303, 1477, 1779, 1884, 2106, 2202
GsaI CCCAGC 2 cut(s) 177, 496
GsuI CTGGAG 1 cut(s) 1629
HaeII RGCGCY 1 cut(s) 964
HapII CCGG 4 cut(s) 107, 239, 610, 1368
HhaI GCGC 5 cut(s) 117, 119, 459, 912, 963
Hin6I GCGC 5 cut(s) 115, 117, 457, 910, 961
HinP1I GCGC 5 cut(s) 115, 117, 457, 910, 961
HincII GTYRAC 2 cut(s) 786, 2069
HindII GTYRAC 2 cut(s) 786, 2069
HindIII AAGCTT 1 cut(s) 1161
HinfI GANTC 8 cut(s) 154, 619, 901, 1185, 1267, 1288, 1573, 1866
HpaII CCGG 4 cut(s) 107, 239, 610, 1368
Hpy166II GTNNAC 6 cut(s) 400, 528, 638, 786, 1689, 2069
Hpy188I TCNGA 9 cut(s) 20, 201, 477, 566, 1031, 1423, 1450, 1865, 2237
Hpy8I GTNNAC 6 cut(s) 400, 528, 638, 786, 1689, 2069
Hpy99I CGWCG 1 cut(s) 1646
HpyAV CCTTC 8 cut(s) 248, 319, 439, 563, 692, 1027, 1740, 1744
HpyCH4III ACNGT 7 cut(s) 779, 1085, 1907, 2018, 2066, 2102, 2141
HpyF10VI GCNNNNNNNGC 3 cut(s) 287, 356, 1139
HpyF3I CTNAG 9 cut(s) 206, 351, 576, 857, 1143, 1347, 1530, 2010, 2076
HspAI GCGC 5 cut(s) 115, 117, 457, 910, 961
Ksp22I TGATCA 1 cut(s) 274
KspI CCGCGG 1 cut(s) 631
Kzo9I GATC 7 cut(s) 274, 886, 1026, 1147, 1423, 1429, 2083
LguI GCTCTTC 2 cut(s) 50, 105
LmnI GCTCC 4 cut(s) 175, 1600, 1610, 1618
Lsp1109I GCAGC 6 cut(s) 274, 289, 1463, 1765, 1870, 2213
LweI GCATC 8 cut(s) 85, 192, 543, 1116, 1183, 1215, 1397, 1534
MaeI CTAG 4 cut(s) 516, 1152, 1182, 1712
MaeIII GTNAC 8 cut(s) 26, 179, 327, 338, 1186, 1497, 1563, 1739
MalI GATC 7 cut(s) 276, 888, 1028, 1149, 1425, 1431, 2085
MbiI CCGCTC 1 cut(s) 134
MboI GATC 7 cut(s) 274, 886, 1026, 1147, 1423, 1429, 2083
MfeI CAATTG 2 cut(s) 2129, 2258
MflI RGATCY 2 cut(s) 1147, 2083
MhlI GDGCHC 6 cut(s) 610, 640, 1261, 1615, 1977, 2233
MlyI GAGTC 3 cut(s) 910, 1194, 1582
MmeI TCCRAC 2 cut(s) 1605, 2111
MseI TTAA 4 cut(s) 12, 1167, 1317, 1758
MslI CAYNNNNRTG 3 cut(s) 918, 1471, 2252
MspA1I CMGCKG 2 cut(s) 287, 630
MspCI CTTAAG 1 cut(s) 1316
MspI CCGG 4 cut(s) 107, 239, 610, 1368
MspR9I CCNGG 6 cut(s) 408, 610, 667, 1597, 1706, 1737
MunI CAATTG 2 cut(s) 2129, 2258
Mva1269I GAATGC 3 cut(s) 322, 361, 2188
MvaI CCWGG 5 cut(s) 408, 667, 1597, 1706, 1737
MvnI CGCG 2 cut(s) 117, 630
MwoI GCNNNNNNNGC 3 cut(s) 287, 356, 1139
NciI CCSGG 1 cut(s) 610
NcoI CCATGG 1 cut(s) 334
NdeII GATC 7 cut(s) 274, 886, 1026, 1147, 1423, 1429, 2083
NlaIV GGNNCC 3 cut(s) 608, 1149, 1602
NmeAIII GCCGAG 2 cut(s) 145, 460
NmuCI GTSAC 8 cut(s) 26, 179, 327, 338, 1186, 1497, 1563, 1739
NspI RCATGY 1 cut(s) 537
OliI CACNNNNGTG 1 cut(s) 2252
PagI TCATGA 2 cut(s) 390, 2175
PauI GCGCGC 1 cut(s) 115
PciI ACATGT 1 cut(s) 533
PciSI GCTCTTC 2 cut(s) 50, 105
PcsI WCGNNNNNNNCGW 1 cut(s) 1680
PctI GAATGC 3 cut(s) 322, 361, 2188
PfeI GAWTC 5 cut(s) 154, 619, 1267, 1288, 1866
PflFI GACNNNGTC 1 cut(s) 1661
PflMI CCANNNNNTGG 1 cut(s) 1583
PkrI GCNGC 8 cut(s) 133, 289, 304, 1478, 1780, 1885, 2107, 2203
PleI GAGTC 3 cut(s) 909, 1193, 1581
PpsI GAGTC 3 cut(s) 909, 1193, 1581
PpuMI RGGWCCY 1 cut(s) 1657
PscI ACATGT 1 cut(s) 533
Psp124BI GAGCTC 1 cut(s) 1615
Psp5II RGGWCCY 1 cut(s) 1657
Psp6I CCWGG 5 cut(s) 406, 665, 1595, 1704, 1735
PspFI CCCAGC 2 cut(s) 173, 492
PspGI CCWGG 5 cut(s) 406, 665, 1595, 1704, 1735
PspN4I GGNNCC 3 cut(s) 608, 1149, 1602
PspOMI GGGCCC 1 cut(s) 606
PspPPI RGGWCCY 1 cut(s) 1657
PsrI GAACNNNNNNTAC 2 cut(s) 1073, 1105
PstI CTGCAG 2 cut(s) 1462, 1783
PstNI CAGNNNCTG 1 cut(s) 2161
PsuI RGATCY 2 cut(s) 1147, 2083
PsyI GACNNNGTC 1 cut(s) 1661
PteI GCGCGC 1 cut(s) 115
PvuII CAGCTG 1 cut(s) 287
RsaI GTAC 7 cut(s) 440, 957, 1314, 1690, 1733, 2020, 2243
RsaNI GTAC 7 cut(s) 439, 956, 1313, 1689, 1732, 2019, 2242
RseI CAYNNNNRTG 3 cut(s) 918, 1471, 2252
SacI GAGCTC 1 cut(s) 1615
SacII CCGCGG 1 cut(s) 631
SalI GTCGAC 1 cut(s) 2067
SapI GCTCTTC 2 cut(s) 50, 105
SaqAI TTAA 4 cut(s) 12, 1167, 1317, 1758
SatI GCNGC 8 cut(s) 132, 288, 303, 1477, 1779, 1884, 2106, 2202
Sau3AI GATC 7 cut(s) 274, 886, 1026, 1147, 1423, 1429, 2083
SchI GAGTC 3 cut(s) 910, 1194, 1582
ScrFI CCNGG 6 cut(s) 408, 610, 667, 1597, 1706, 1737
SduI GDGCHC 6 cut(s) 610, 640, 1261, 1615, 1977, 2233
SfaNI GCATC 8 cut(s) 85, 192, 543, 1116, 1183, 1215, 1397, 1534
SfcI CTRYAG 4 cut(s) 778, 1458, 1568, 1779
Sfr303I CCGCGG 1 cut(s) 631
SgrBI CCGCGG 1 cut(s) 631
SinI GGWCC 3 cut(s) 729, 1307, 1657
SmiMI CAYNNNNRTG 3 cut(s) 918, 1471, 2252
SmlI CTYRAG 2 cut(s) 1316, 1652
SmoI CTYRAG 2 cut(s) 1316, 1652
SsiI CCGC 5 cut(s) 132, 628, 630, 2106, 2245
SspI AATATT 1 cut(s) 644
SspMI CTAG 4 cut(s) 516, 1152, 1182, 1712
SstI GAGCTC 1 cut(s) 1615
StyD4I CCNGG 6 cut(s) 406, 608, 665, 1595, 1704, 1735
StyI CCWWGG 2 cut(s) 334, 2041
TaaI ACNGT 7 cut(s) 779, 1085, 1907, 2018, 2066, 2102, 2141
TaqI TCGA 6 cut(s) 87, 586, 1644, 1683, 1929, 2068
TatI WGTACW 5 cut(s) 438, 955, 1312, 1688, 2018
TauI GCSGC 2 cut(s) 134, 2108
TfiI GAWTC 5 cut(s) 154, 619, 1267, 1288, 1866
Tru1I TTAA 4 cut(s) 12, 1167, 1317, 1758
Tru9I TTAA 4 cut(s) 12, 1167, 1317, 1758
TscAI CASTG 4 cut(s) 184, 347, 1195, 1462
TseFI GTSAC 8 cut(s) 26, 179, 327, 338, 1186, 1497, 1563, 1739
TseI GCWGC 6 cut(s) 287, 302, 1476, 1778, 1883, 2201
Tsp45I GTSAC 8 cut(s) 26, 179, 327, 338, 1186, 1497, 1563, 1739
TspDTI ATGAA 9 cut(s) 743, 1055, 1314, 1373, 1683, 1756, 1806, 2135, 2164
TspGWI ACGGA 1 cut(s) 1379
TspRI CASTG 4 cut(s) 184, 347, 1195, 1462
Tth111I GACNNNGTC 1 cut(s) 1661
Van91I CCANNNNNTGG 1 cut(s) 1583
Vha464I CTTAAG 1 cut(s) 1316
VneI GTGCAC 1 cut(s) 636
VpaK11BI GGWCC 3 cut(s) 729, 1307, 1657
XagI CCTNNNNNAGG 2 cut(s) 96, 601
XapI RAATTY 5 cut(s) 142, 231, 1062, 1301, 1646
XceI RCATGY 1 cut(s) 537
XcmI CCANNNNNNNNNTGG 1 cut(s) 1808
XmiI GTMKAC 2 cut(s) 399, 2068
XspI CTAG 4 cut(s) 516, 1152, 1182, 1712
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.