Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0000173

Physical Location & Seq

Genomic Coordinates Forward (+)

29454974 .. 29460150

Gene Structure 5177 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1246300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1128 bp

ATGTGGAAGCTTAAGGTTGCAGATGGTGGCAATGATCCATACATCTACAGCACAAACAACTTCGTGGGTAGGCAGATATTTGAGTTTGACCCTGAAGCAGGAACTACCGAAGAGCGAGCTAAGGTGGAAGAAGCTCGTCTTCATTTCTACAATAATCGCTATCAGGTTAAGCCCAATAGTGACCTCCTATGGCGAATGCAGTTCCTAAGAGAGAAGAACTTCAAACAGACAATTCCCCCAGTGAAAGTTGAGGATGGAGAGGAAATCACATACGAAAAGGCCACCGCTGCATTGAGAAGGTCTGTCCACTTCTTTTCAGCCTTGCAAGCTAGTGATGGCCATTGGCCGGCAGAAAATGCTGGCCCGTTGTTTTTCCTTCCTCCTTTGGTCATGTGCACTTACATTACGGGACATCTTAATACTATACTCCCTGCAGAACACCGCAAAGAAATTTTGCGTTACATATACTATCATCAGATGTTGAATCCCACAGAATTCTTGGCGGACATTGTGGTTGAGCATGAATACGTTGAGTGCACTTCATCTGCAATCCAGGCTTTAGTTTTGTTTAAGAAGTTATACCCTGGCCATAGGAAGGAAGAGATTGATCAATTTATTACCGCTGCTGCACTGTACCTTGAAAATGTACAAATGGCAGATGGTTCATGGTACGGGAATTGGGGAGTTTGCTTCACATATGGTACCTGGTTTGCTCTTGGAGGGCTAACTGCTGCTGGTAAGACCTTCAACAATTGTGCAGCCATGCGCAAAGCTATTAGTTTTCTACTCGCAATCCAGAAAGAGAATGGTGGTTGGGGAGAGAGCTACCTTTCATGTCCAAAAAAGGAGTACGTTCCTCTTGAAGGAAACCGATCAAATTTAGTACACACAGCTTGGGCTATGATGGGTTTGATTCATGCAGGGCAGGCGGAAAGAGACCCCACACCCCTTCATCGTGCAGCAAAGTTGATAATCAATTCTCAGATGGAAAATGGTGATTTTCCTCAGCAGGAAATCACTGGAGTATTCTTGAAGAACTGCATGCTACATTATGCAGCTTATAGAAATATCTTTCCCATGTGGGCACTTGCAGAATACCGCAGGCGGGTTCCGTTCGTTGATGTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006629 GO:0006720 GO:0006721 GO:0006722 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009987 GO:0016104 GO:0016114 GO:0016853 GO:0016866 GO:0019742 GO:0019745 GO:0031559 GO:0042300 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0071704 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00909 ko01110 map00909 map01110

Pfam Domains

Protein Families

SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

376

Amino Acids

43.0

Weight (kDa)

6.47

Isoelectric Point (pI)

35.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SQHop_cyclase_C	PF13243	200 - 368	7.6e-37	Squalene-hopene cyclase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000173)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G66960 AT1G66960 AT1G66960 AT1G66960 AT1G78950 AT1G78950 AT1G78955 AT1G78955 AT1G78955 AT1G78955 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78970 AT1G78970 AT1G78970
fragaria_vesca	FvH4_5g29480 FvH4_6g36450 FvH4_6g36450 FvH4_6g36500 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g37620
malus_domestica	MD03G1089600.v1.1 MD09G1167700.v1.1 MD09G1168200.v1.1 MD09G1168300.v1.1 MD11G1098600.v1.1 MD17G1158300.v1.1 MD17G1158700.v1.1 MD17G1159500.v1.1 MD17G1160500.v1.1 MD17G1182500.v1.1 MD17G1182700.v1.1 MD17G1245800.v1.1 MD17G1246300.v1.1 MD17G1246600.v1.1
prunus_persica	Prupe.3G025700_v2.0.a1 Prupe.3G025700_v2.0.a1 Prupe.3G025800_v2.0.a1 Prupe.3G025900_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026400_v2.0.a1 Prupe.3G026500_v2.0.a1 Prupe.3G026700_v2.0.a1 Prupe.3G026800_v2.0.a1
pyrus_communis	pycom03g07120 pycom09g08520 pycom09g08590 pycom09g08610 pycom10g10070 pycom11g08310 pycom16g19880 pycom17g15190 pycom17g15300
rosa_chinensis	RchiOBHm_Chr2g0136221 RchiOBHm_Chr2g0148451 RchiOBHm_Chr2g0148501 RchiOBHm_Chr2g0148521 RchiOBHm_Chr2g0148661 RchiOBHm_Chr2g0148701 RchiOBHm_Chr2g0148811 RchiOBHm_Chr2g0148941 RchiOBHm_Chr2g0148981 RchiOBHm_Chr2g0150451 RchiOBHm_Chr2g0150521 RchiOBHm_Chr2g0150611 RchiOBHm_Chr4g0420831 RchiOBHm_Chr5g0049791 RchiOBHm_Chr5g0049801
rosa_laevigata	RLG00000007735 RLG00000020351 RLG00000020352 RLG00000020357 RLG00000020358 RLG00000020360 RLG00000029093 RLG00000030686
rosa_multiflora	Rmu_co8274513.1_g000001 Rmu_sc0000048.1_g000003 Rmu_sc0000048.1_g000033 Rmu_sc0000048.1_g000039 Rmu_sc0001397.1_g000011 Rmu_sc0001998.1_g000039 Rmu_sc0002070.1_g000055 Rmu_sc0002082.1_g000052 Rmu_sc0003181.1_g000010 Rmu_sc0005104.1_g000005 Rmu_sc0006758.1_g000002 Rmu_sc0008411.1_g000029 Rmu_sc0008411.1_g000030 Rmu_sc0017472.1_g000001 Rmu_sc0017472.1_g000003 Rmu_sc0029876.1_g000001 Rmu_ssc0000480.1_g000024 Rmu_ssc0000480.1_g000036
rosa_roxburghii	Rroxscaffold_2G00097070 Rroxscaffold_2G00098540 Rroxscaffold_2G00098560 Rroxscaffold_2G00098570 Rroxscaffold_2G00098650 Rroxscaffold_2G00098710 Rroxscaffold_2G00098740 Rroxscaffold_3G00225830 Rroxscaffold_3G00236870 Rroxscaffold_4G00312240
rosa_rugosa	Rorug01G0047000 Rorug02G0372300 Rorug02G0407200 Rorug02G0407300 Rorug02G0408400 Rorug02G0421500 Rorug07G0208900
rosa_samantha	Rh2AG380200 Rh2AG465700 Rh2AG465800 Rh2AG466800 Rh2AG467100 Rh2AG467600 Rh2AG480900 Rh2AG481400 Rh2AG481700 Rh2CG073500 Rh2CG452200 Rh2CG453200 Rh2CG453600 Rh2CG454100 Rh2CG467100 Rh2CG467800 Rh2CG468000 Rh2DG403100 Rh2DG487800 Rh2DG487900 Rh2DG488800 Rh2DG502000 Rh2DG502200 Rh2DG502300 Rh2DG503100 Rh3AG319300 Rh4AG227700 Rh4BG371800 Rh5AG327400 Rh5DG350600 Rh7AG349800
rosa_wichuraiana	Rw0G010080 Rw2G031110 Rw2G037980 Rw2G037990 Rw2G038000 Rw2G038010 Rw2G038080 Rw2G038100 Rw2G039260 Rw2G039290 Rw2G039470 Rw4G019710 Rw5G030930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	767
Acc65I	GGTACC	1 cut(s)	701
AccB1I	GGYRCC	1 cut(s)	701
AciI	CCGC	7 cut(s)	285, 442, 503, 621, 929, 1099, 1105
AclWI	GGATC	1 cut(s)	29
AcoI	YGGCCR	3 cut(s)	337, 344, 586
AcsI	RAATTY	3 cut(s)	450, 494, 877
AcuI	CTGAAG	1 cut(s)	114
AfaI	GTAC	6 cut(s)	635, 648, 671, 703, 851, 885
AfiI	CCNNNNNNNGG	5 cut(s)	98, 346, 595, 863, 1105
AflII	CTTAAG	1 cut(s)	11
AgsI	TTSAA	6 cut(s)	223, 484, 641, 748, 863, 1033
AjnI	CCWGG	3 cut(s)	552, 583, 704
AluBI	AGCT	8 cut(s)	10, 119, 134, 329, 773, 825, 893, 1058
AluI	AGCT	8 cut(s)	10, 119, 134, 329, 773, 825, 893, 1058
Alw21I	GWGCWC	2 cut(s)	398, 539
Alw26I	GTCTC	1 cut(s)	930
Alw44I	GTGCAC	2 cut(s)	394, 535
AlwI	GGATC	1 cut(s)	29
AoxI	GGCC	5 cut(s)	279, 337, 344, 361, 586
ApaLI	GTGCAC	2 cut(s)	394, 535
ApeKI	GCWGC	7 cut(s)	287, 623, 626, 731, 758, 959, 1055
ApoI	RAATTY	3 cut(s)	450, 494, 877
Asp700I	GAANNNNTTC	1 cut(s)	218
Asp718I	GGTACC	1 cut(s)	701
AspLEI	GCGC	1 cut(s)	768
AspS9I	GGNCC	1 cut(s)	362
AsuHPI	GGTGA	1 cut(s)	1007
BaeGI	GKGCMC	3 cut(s)	398, 539, 1087
BalI	TGGCCA	2 cut(s)	339, 588
BanI	GGYRCC	1 cut(s)	701
BbsI	GAAGAC	1 cut(s)	131
Bbv12I	GWGCWC	2 cut(s)	398, 539
BbvCI	CCTCAGC	1 cut(s)	1005
BbvI	GCAGC	7 cut(s)	274, 610, 613, 718, 770, 971, 1067
BccI	CCATC	6 cut(s)	17, 248, 329, 653, 898, 979
BciT130I	CCWGG	3 cut(s)	554, 585, 706
BclI	TGATCA	1 cut(s)	607
BcoDI	GTCTC	1 cut(s)	930
BfaI	CTAG	1 cut(s)	330
BfmI	CTRYAG	2 cut(s)	46, 432
BfrI	CTTAAG	1 cut(s)	11
BisI	GCNGC	7 cut(s)	288, 624, 627, 732, 759, 960, 1056
BlsI	GCNGC	7 cut(s)	289, 625, 628, 733, 760, 961, 1057
Bme1390I	CCNGG	3 cut(s)	554, 585, 706
BmgT120I	GGNCC	1 cut(s)	362
BmiI	GGNNCC	2 cut(s)	703, 1110
BmrFI	CCNGG	3 cut(s)	554, 585, 706
BmrI	ACTGGG	1 cut(s)	233
BmuI	ACTGGG	1 cut(s)	233
BpiI	GAAGAC	1 cut(s)	131
BpmI	CTGGAG	1 cut(s)	1041
Bpu10I	CCTNAGC	2 cut(s)	120, 1005
BsaI	GGTCTC	1 cut(s)	930
BsaJI	CCNNGG	1 cut(s)	583
Bsc4I	CCNNNNNNNGG	5 cut(s)	98, 346, 595, 863, 1105
Bse118I	RCCGGY	1 cut(s)	346
Bse1I	ACTGG	2 cut(s)	239, 1024
Bse3DI	GCAATG	1 cut(s)	37
BseBI	CCWGG	3 cut(s)	554, 585, 706
BseDI	CCNNGG	1 cut(s)	583
BseGI	GGATG	1 cut(s)	259
BseLI	CCNNNNNNNGG	5 cut(s)	98, 346, 595, 863, 1105
BseMI	GCAATG	1 cut(s)	37
BseMII	CTCAG	2 cut(s)	995, 1019
BseNI	ACTGG	2 cut(s)	239, 1024
BseSI	GKGCMC	3 cut(s)	398, 539, 1087
BseXI	GCAGC	7 cut(s)	274, 610, 613, 718, 770, 971, 1067
BsgI	GTGCAG	3 cut(s)	612, 777, 978
BshFI	GGCC	5 cut(s)	281, 339, 346, 363, 588
BshNI	GGYRCC	1 cut(s)	701
BsiHKAI	GWGCWC	2 cut(s)	398, 539
BsiSI	CCGG	1 cut(s)	347
BslFI	GGGAC	1 cut(s)	423
BslI	CCNNNNNNNGG	5 cut(s)	98, 346, 595, 863, 1105
BsmAI	GTCTC	1 cut(s)	930
BsmFI	GGGAC	1 cut(s)	423
BsmI	GAATGC	1 cut(s)	201
BsnI	GGCC	5 cut(s)	281, 339, 346, 363, 588
Bso31I	GGTCTC	1 cut(s)	930
Bsp1286I	GDGCHC	3 cut(s)	398, 539, 1087
Bsp1407I	TGTACA	1 cut(s)	646
Bsp143I	GATC	3 cut(s)	34, 607, 872
BspACI	CCGC	7 cut(s)	285, 442, 503, 621, 929, 1099, 1105
BspANI	GGCC	5 cut(s)	281, 339, 346, 363, 588
BspCNI	CTCAG	2 cut(s)	994, 1018
BspLI	GGNNCC	2 cut(s)	703, 1110
BspMAI	CTGCAG	1 cut(s)	436
BspPI	GGATC	1 cut(s)	29
BspQI	GCTCTTC	1 cut(s)	105
BspT107I	GGYRCC	1 cut(s)	701
BspTI	CTTAAG	1 cut(s)	11
BspTNI	GGTCTC	1 cut(s)	930
BsrDI	GCAATG	1 cut(s)	37
BsrFI	RCCGGY	1 cut(s)	346
BsrGI	TGTACA	1 cut(s)	646
BsrI	ACTGG	2 cut(s)	239, 1024
BssAI	RCCGGY	1 cut(s)	346
BssECI	CCNNGG	1 cut(s)	583
BssMI	GATC	3 cut(s)	34, 607, 872
Bst2UI	CCWGG	3 cut(s)	554, 585, 706
Bst4CI	ACNGT	1 cut(s)	633
Bst6I	CTCTTC	2 cut(s)	105, 594
BstAFI	CTTAAG	1 cut(s)	11
BstAPI	GCANNNNNTGC	1 cut(s)	356
BstAUI	TGTACA	1 cut(s)	646
BstC8I	GCNNGC	7 cut(s)	117, 327, 348, 361, 927, 1043, 1103
BstDEI	CTNAG	4 cut(s)	120, 206, 981, 1005
BstENI	CCTNNNNNAGG	2 cut(s)	96, 861
BstF5I	GGATG	1 cut(s)	259
BstHHI	GCGC	1 cut(s)	768
BstKTI	GATC	3 cut(s)	37, 610, 875
BstMAI	GTCTC	1 cut(s)	930
BstMBI	GATC	3 cut(s)	34, 607, 872
BstMWI	GCNNNNNNNGC	5 cut(s)	287, 326, 356, 554, 926
BstNI	CCWGG	3 cut(s)	554, 585, 706
BstNSI	RCATGY	1 cut(s)	1045
BstSCI	CCNGG	3 cut(s)	552, 583, 704
BstSFI	CTRYAG	2 cut(s)	46, 432
BstSLI	GKGCMC	3 cut(s)	398, 539, 1087
BstV1I	GCAGC	7 cut(s)	274, 610, 613, 718, 770, 971, 1067
BstV2I	GAAGAC	1 cut(s)	131
BsuRI	GGCC	5 cut(s)	281, 339, 346, 363, 588
BtsCI	GGATG	1 cut(s)	259
BtsIMutI	CAGTG	3 cut(s)	246, 629, 1017
Cac8I	GCNNGC	7 cut(s)	117, 327, 348, 361, 927, 1043, 1103
CfoI	GCGC	1 cut(s)	768
Cfr10I	RCCGGY	1 cut(s)	346
Cfr13I	GGNCC	1 cut(s)	362
CsiI	ACCWGGT	1 cut(s)	704
Csp6I	GTAC	6 cut(s)	634, 647, 670, 702, 850, 884
CviAII	CATG	8 cut(s)	391, 521, 666, 763, 834, 917, 1042, 1078
CviQI	GTAC	6 cut(s)	634, 647, 670, 702, 850, 884
DdeI	CTNAG	4 cut(s)	120, 206, 981, 1005
DpnI	GATC	3 cut(s)	36, 609, 874
DpnII	GATC	3 cut(s)	34, 607, 872
EaeI	YGGCCR	3 cut(s)	337, 344, 586
Eam1104I	CTCTTC	2 cut(s)	105, 594
EarI	CTCTTC	2 cut(s)	105, 594
EciI	GGCGGA	2 cut(s)	518, 944
Eco31I	GGTCTC	1 cut(s)	930
Eco57I	CTGAAG	1 cut(s)	114
EcoNI	CCTNNNNNAGG	2 cut(s)	96, 861
EcoRI	GAATTC	1 cut(s)	494
EcoRII	CCWGG	3 cut(s)	552, 583, 704
FaeI	CATG	8 cut(s)	394, 524, 669, 766, 837, 920, 1045, 1081
FalI	AAGNNNNNCTT	2 cut(s)	123, 155
FaqI	GGGAC	1 cut(s)	423
FatI	CATG	8 cut(s)	390, 520, 665, 762, 833, 916, 1041, 1077
FauI	CCCGC	1 cut(s)	1098
FauNDI	CATATG	1 cut(s)	697
FbaI	TGATCA	1 cut(s)	607
Fnu4HI	GCNGC	7 cut(s)	288, 624, 627, 732, 759, 960, 1056
FokI	GGATG	1 cut(s)	266
Fsp4HI	GCNGC	7 cut(s)	288, 624, 627, 732, 759, 960, 1056
FspBI	CTAG	1 cut(s)	330
FspI	TGCGCA	1 cut(s)	767
GlaI	GCGC	1 cut(s)	767
GluI	GCNGC	7 cut(s)	288, 624, 627, 732, 759, 960, 1056
GsuI	CTGGAG	1 cut(s)	1041
HaeIII	GGCC	5 cut(s)	281, 339, 346, 363, 588
HapII	CCGG	1 cut(s)	347
HhaI	GCGC	1 cut(s)	768
Hin1II	CATG	8 cut(s)	394, 524, 669, 766, 837, 920, 1045, 1081
Hin6I	GCGC	1 cut(s)	766
HinP1I	GCGC	1 cut(s)	766
HindIII	AAGCTT	1 cut(s)	8
HinfI	GANTC	2 cut(s)	484, 913
HpaII	CCGG	1 cut(s)	347
HphI	GGTGA	1 cut(s)	1007
Hpy166II	GTNNAC	4 cut(s)	307, 396, 537, 886
Hpy188I	TCNGA	2 cut(s)	477, 984
Hpy188III	TCNNGA	3 cut(s)	796, 860, 1030
Hpy8I	GTNNAC	4 cut(s)	307, 396, 537, 886
HpyAV	CCTTC	6 cut(s)	291, 386, 589, 754, 857, 959
HpyCH4III	ACNGT	1 cut(s)	633
HpyCH4IV	ACGT	2 cut(s)	528, 852
HpyF10VI	GCNNNNNNNGC	5 cut(s)	287, 326, 356, 554, 926
HpyF3I	CTNAG	4 cut(s)	120, 206, 981, 1005
HpySE526I	ACGT	2 cut(s)	528, 852
Hsp92II	CATG	8 cut(s)	394, 524, 669, 766, 837, 920, 1045, 1081
HspAI	GCGC	1 cut(s)	766
KpnI	GGTACC	1 cut(s)	705
KroI	GCCGGC	1 cut(s)	346
KroNI	GCCGGC	1 cut(s)	348
Ksp22I	TGATCA	1 cut(s)	607
Kzo9I	GATC	3 cut(s)	34, 607, 872
LguI	GCTCTTC	1 cut(s)	105
Lsp1109I	GCAGC	7 cut(s)	274, 610, 613, 718, 770, 971, 1067
MabI	ACCWGGT	1 cut(s)	704
MaeI	CTAG	1 cut(s)	330
MaeII	ACGT	2 cut(s)	528, 852
MaeIII	GTNAC	2 cut(s)	179, 458
MalI	GATC	3 cut(s)	36, 609, 874
MboI	GATC	3 cut(s)	34, 607, 872
MboII	GAAGA	6 cut(s)	122, 131, 140, 226, 611, 1045
MfeI	CAATTG	1 cut(s)	751
MhlI	GDGCHC	3 cut(s)	398, 539, 1087
MlsI	TGGCCA	2 cut(s)	339, 588
MluCI	AATT	8 cut(s)	231, 450, 494, 611, 676, 751, 877, 976
MluNI	TGGCCA	2 cut(s)	339, 588
MnlI	CCTC	7 cut(s)	194, 244, 253, 390, 713, 867, 1014
Mox20I	TGGCCA	2 cut(s)	339, 588
MroNI	GCCGGC	1 cut(s)	346
MroXI	GAANNNNTTC	1 cut(s)	218
MscI	TGGCCA	2 cut(s)	339, 588
MseI	TTAA	4 cut(s)	12, 168, 417, 570
Msp20I	TGGCCA	2 cut(s)	339, 588
MspA1I	CMGCKG	2 cut(s)	287, 623
MspCI	CTTAAG	1 cut(s)	11
MspI	CCGG	1 cut(s)	347
MspR9I	CCNGG	3 cut(s)	554, 585, 706
MunI	CAATTG	1 cut(s)	751
Mva1269I	GAATGC	1 cut(s)	201
MvaI	CCWGG	3 cut(s)	554, 585, 706
MwoI	GCNNNNNNNGC	5 cut(s)	287, 326, 356, 554, 926
NaeI	GCCGGC	1 cut(s)	348
NdeI	CATATG	1 cut(s)	697
NdeII	GATC	3 cut(s)	34, 607, 872
NgoMIV	GCCGGC	1 cut(s)	346
NlaIII	CATG	8 cut(s)	394, 524, 669, 766, 837, 920, 1045, 1081
NlaIV	GGNNCC	2 cut(s)	703, 1110
NmuCI	GTSAC	1 cut(s)	179
NsbI	TGCGCA	1 cut(s)	767
NspI	RCATGY	1 cut(s)	1045
PaeI	GCATGC	1 cut(s)	1045
PciSI	GCTCTTC	1 cut(s)	105
PctI	GAATGC	1 cut(s)	201
PdiI	GCCGGC	1 cut(s)	348
PdmI	GAANNNNTTC	1 cut(s)	218
PfeI	GAWTC	2 cut(s)	484, 913
PkrI	GCNGC	7 cut(s)	289, 625, 628, 733, 760, 961, 1057
Psp6I	CCWGG	3 cut(s)	552, 583, 704
PspGI	CCWGG	3 cut(s)	552, 583, 704
PspN4I	GGNNCC	2 cut(s)	703, 1110
PspPI	GGNCC	1 cut(s)	362
PstI	CTGCAG	1 cut(s)	436
RsaI	GTAC	6 cut(s)	635, 648, 671, 703, 851, 885
RsaNI	GTAC	6 cut(s)	634, 647, 670, 702, 850, 884
SapI	GCTCTTC	1 cut(s)	105
SaqAI	TTAA	4 cut(s)	12, 168, 417, 570
SatI	GCNGC	7 cut(s)	288, 624, 627, 732, 759, 960, 1056
Sau3AI	GATC	3 cut(s)	34, 607, 872
Sau96I	GGNCC	1 cut(s)	362
ScrFI	CCNGG	3 cut(s)	554, 585, 706
SduI	GDGCHC	3 cut(s)	398, 539, 1087
SexAI	ACCWGGT	1 cut(s)	704
SfcI	CTRYAG	2 cut(s)	46, 432
SmlI	CTYRAG	1 cut(s)	11
SmoI	CTYRAG	1 cut(s)	11
SphI	GCATGC	1 cut(s)	1045
Sse9I	AATT	8 cut(s)	231, 450, 494, 611, 676, 751, 877, 976
SsiI	CCGC	7 cut(s)	285, 442, 503, 621, 929, 1099, 1105
SspMI	CTAG	1 cut(s)	330
StyD4I	CCNGG	3 cut(s)	552, 583, 704
TaaI	ACNGT	1 cut(s)	633
TaiI	ACGT	2 cut(s)	531, 855
TasI	AATT	8 cut(s)	231, 450, 494, 611, 676, 751, 877, 976
TatI	WGTACW	2 cut(s)	646, 883
TfiI	GAWTC	2 cut(s)	484, 913
Tru1I	TTAA	4 cut(s)	12, 168, 417, 570
Tru9I	TTAA	4 cut(s)	12, 168, 417, 570
TscAI	CASTG	3 cut(s)	246, 636, 1024
TseFI	GTSAC	1 cut(s)	179
TseI	GCWGC	7 cut(s)	287, 623, 626, 731, 758, 959, 1055
Tsp45I	GTSAC	1 cut(s)	179
TspDTI	ATGAA	7 cut(s)	131, 531, 537, 654, 822, 905, 941
TspGWI	ACGGA	1 cut(s)	1101
TspRI	CASTG	3 cut(s)	246, 636, 1024
Vha464I	CTTAAG	1 cut(s)	11
VneI	GTGCAC	2 cut(s)	394, 535
XagI	CCTNNNNNAGG	2 cut(s)	96, 861
XapI	RAATTY	3 cut(s)	450, 494, 877
XceI	RCATGY	1 cut(s)	1045
XcmI	CCANNNNNNNNNTGG	2 cut(s)	496, 803
XmnI	GAANNNNTTC	1 cut(s)	218
XspI	CTAG	1 cut(s)	330

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.