Rw2G031110

Belongs to the terpene cyclase mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000173
Physical Location & Seq
Reverse (-)
51131887 .. 51145427
13541 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G031110.1

Sequence Viewer

Length: 2229 bp
ATGTGGAAGCTTAGGTTTGGAAACGAAGGTGCAAATAATAACCCTTACAGTCCTTACTTGTTTAGCACAAACAACTTTTATGGGAGGCAAACATGGGAGTTTGACCCGGCTGCCGGTACGCCTGAAGAGCGAGCAGAGGTCGAAGAAGTTCGACTAAGTTTTTACAACAATCGTCACCAGGTCCAGGCTTGCAGTGACCTCCTCTGCCGAATGCAGAAAAAGTTCAAACAAAAGATTCCCCAAGTAAAGTTGGAGGATGGTGAGGACTGTACATATGAAAAGGCCACCGCTGCATTCACAAGGGCAGCTACCTTCTACTCTGCCTTGCAATCACCTCACGGCCATTGGCCTGCTGAACATGCCGGTCTCATGTTTTATATTCCTTCCTTGGTCATACCGCTCTATATTATGGGGCATCTTAGTAAGGTGTTCTCTGCAGAGCATTATAAGGAAATGCTTAGATATATATACTGTCATCAGAACAAGGATGGTGGGTGGGGATTACACATACAGAGCCCTAGTATGATGTATTGTACACTTCTGAACTATATTTGCATGCGTCTACTTGGAGAAGGACCTGATGGTGGTTTAAACAACGCTTGTGCAAGAGCACGCAAGTGGATTCTTGATCATGGTGGTGCAACACATTCATCATCCTGGGGAAAGACTTTTATGGCAATACTTGGTGTGTATGAGTGGGAGGGATGCAACCCGATTCCCCCTGCGTTTTGGATTTTCCCGTCTTTGTTCCCTTTTCATCCAGGTTTATTTGTTGCCCCCATCACCCCTCTCATTCTACAACTGAGAGAAGAAATGTACAATGAGCCATACAATGAAATTCAATGGGGCAAAGTACGCCATCTTTGTGCAAAGGAGGATAACTACAATCCCCATGGCAAGTTACAATGTTTGTTGTGGGATACTCTGAACACAGTTTATGAGCCTCTTCTTTCTTGCTGGCCCTTCAATAAGTTGAGAGAGAAGGCTCTTCAAGAAACTATTGAACACATTCATTATGAAGATGAAAATAGTCGATACATAACAATTGGATGTGCGGAAAAGCCATTAATGATGCTTGCTTGCTGGATTGAAGATCCGAATGGAGAACCCTTTAAGAAGCATCTTGCAAGAGTTATAGATTACATATGGGTTGCAGAAGATGGAATAACTACGCAGAGTTTTGGTAGCCAGGCATGGGATGCTAGTCTTTCTGTTCAAGCTTTGCTTGCTGGAAATCTCACTGATGAACTTGGATCTGTACTTCAGAAAGCACATGAATTCCTAAAAAAATCTCAGGTTAGGGATAATCCTTCTGGTGACTTCTTAGCTCATTTCCGTCACATTTCTAAAGGTGCTTGGACTTTGTCTGACCGAGATCATGGATGGCAAGTTTCTGATTGTACTGCAGAGGGTTTGAAGTGTTGCCTATTGTTTTCAATGATGCCGACGCAAATCGTGGGTGAACCAATTGACTCTGAGCGCTTTTATGATGCTGTCAATGTCATACTTTCTTTGCAAAATCCAAATGGTGGTGTATCAGCCTGGCAGCCAACAGGAGCTCCACAATGGTTGGAGTGGTTCAATCCAGTAGAATTTCTCGAGAAAGTCGTCATTGAATACGAGTATGTTGAGTGCACTTCATCTTCAATTCAAGCGTTAGCTTTATTCAGGAAGTTATACCCTGGGCATAGAAAGAAACATATCGATGATTTCATCACAAATGCAGCATGTTACCTTGAAGATATGCAGACACCTGATGGCTCATGGTATGGAATATGGGGAATCTGCTTCGTCTATGGAACATGGTTTGCGATTCTAGGGTTGAAGGCTGCTGGTAGAACTTACAAAAATTGTGAGGCGATACGCAGAGGTGTTGAATTTTTACTCAAAATACAGAGAAATGATGGTGGTTGGGGAGAGAGCTATACTTCTTGTACAAACAAGATCTATACACCTCTTGAAGGCGAACGGTCAAATTTGGTGCAGACTGCAATAGGACTAATGGGATTAATTCATGGTGGACAGGCCGAGAGAGACCCTACTCCCATTCACAGAGCTGCGAAGCTTTTGGTTAATTCTCAATTGGAAAATGGAGATTTCCCCCAACAGGAACTGATGGGGGCTGCCAATGTCAATATCATGTTACACTATGCAGCATATAGGAATATCTTCCCAATTTGGGCTCTCGGAGAATATCGTTCCCTTCCCTCCTTCTGGATAGATGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 ko01110 map00909 map01110
Pfam Domains
Protein Families
SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

742

Amino Acids

84.62

Weight (kDa)

5.84

Isoelectric Point (pI)

45.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQHop_cyclase_N PF13249 101 - 342 3.1e-26 Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C PF13243 396 - 733 2e-44 Squalene-hopene cyclase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66960 AT1G66960 AT1G66960 AT1G66960 AT1G78950 AT1G78950 AT1G78955 AT1G78955 AT1G78955 AT1G78955 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78970 AT1G78970 AT1G78970
fragaria_vesca FvH4_5g29480 FvH4_6g36450 FvH4_6g36450 FvH4_6g36500 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g37620
malus_domestica MD03G1089600.v1.1 MD09G1167700.v1.1 MD09G1168200.v1.1 MD09G1168300.v1.1 MD11G1098600.v1.1 MD17G1158300.v1.1 MD17G1158700.v1.1 MD17G1159500.v1.1 MD17G1160500.v1.1 MD17G1182500.v1.1 MD17G1182700.v1.1 MD17G1245800.v1.1 MD17G1246300.v1.1 MD17G1246600.v1.1
prunus_persica Prupe.3G025700_v2.0.a1 Prupe.3G025700_v2.0.a1 Prupe.3G025800_v2.0.a1 Prupe.3G025900_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026400_v2.0.a1 Prupe.3G026500_v2.0.a1 Prupe.3G026700_v2.0.a1 Prupe.3G026800_v2.0.a1
pyrus_communis pycom03g07120 pycom09g08520 pycom09g08590 pycom09g08610 pycom10g10070 pycom11g08310 pycom16g19880 pycom17g15190 pycom17g15300
rosa_chinensis RchiOBHm_Chr2g0136221 RchiOBHm_Chr2g0148451 RchiOBHm_Chr2g0148501 RchiOBHm_Chr2g0148521 RchiOBHm_Chr2g0148661 RchiOBHm_Chr2g0148701 RchiOBHm_Chr2g0148811 RchiOBHm_Chr2g0148941 RchiOBHm_Chr2g0148981 RchiOBHm_Chr2g0150451 RchiOBHm_Chr2g0150521 RchiOBHm_Chr2g0150611 RchiOBHm_Chr4g0420831 RchiOBHm_Chr5g0049791 RchiOBHm_Chr5g0049801
rosa_laevigata RLG00000007735 RLG00000020351 RLG00000020352 RLG00000020357 RLG00000020358 RLG00000020360 RLG00000029093 RLG00000030686
rosa_multiflora Rmu_co8274513.1_g000001 Rmu_sc0000048.1_g000003 Rmu_sc0000048.1_g000033 Rmu_sc0000048.1_g000039 Rmu_sc0001397.1_g000011 Rmu_sc0001998.1_g000039 Rmu_sc0002070.1_g000055 Rmu_sc0002082.1_g000052 Rmu_sc0003181.1_g000010 Rmu_sc0005104.1_g000005 Rmu_sc0006758.1_g000002 Rmu_sc0008411.1_g000029 Rmu_sc0008411.1_g000030 Rmu_sc0017472.1_g000001 Rmu_sc0017472.1_g000003 Rmu_sc0029876.1_g000001 Rmu_ssc0000480.1_g000024 Rmu_ssc0000480.1_g000036
rosa_roxburghii Rroxscaffold_2G00097070 Rroxscaffold_2G00098540 Rroxscaffold_2G00098560 Rroxscaffold_2G00098570 Rroxscaffold_2G00098650 Rroxscaffold_2G00098710 Rroxscaffold_2G00098740 Rroxscaffold_3G00225830 Rroxscaffold_3G00236870 Rroxscaffold_4G00312240
rosa_rugosa Rorug01G0047000 Rorug02G0372300 Rorug02G0407200 Rorug02G0407300 Rorug02G0408400 Rorug02G0421500 Rorug07G0208900
rosa_samantha Rh2AG380200 Rh2AG465700 Rh2AG465800 Rh2AG466800 Rh2AG467100 Rh2AG467600 Rh2AG480900 Rh2AG481400 Rh2AG481700 Rh2CG073500 Rh2CG452200 Rh2CG453200 Rh2CG453600 Rh2CG454100 Rh2CG467100 Rh2CG467800 Rh2CG468000 Rh2DG403100 Rh2DG487800 Rh2DG487900 Rh2DG488800 Rh2DG502000 Rh2DG502200 Rh2DG502300 Rh2DG503100 Rh3AG319300 Rh4AG227700 Rh4BG371800 Rh5AG327400 Rh5DG350600 Rh7AG349800
rosa_wichuraiana Rw0G010080 Rw2G031110 Rw2G037980 Rw2G037990 Rw2G038000 Rw2G038010 Rw2G038080 Rw2G038100 Rw2G039260 Rw2G039290 Rw2G039470 Rw4G019710 Rw5G030930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 447
AccB7I CCANNNNNTGG 1 cut(s) 1529
AccBSI CCGCTC 1 cut(s) 400
AccI GTMKAC 1 cut(s) 562
AciI CCGC 3 cut(s) 288, 398, 1055
AclWI GGATC 2 cut(s) 1088, 1261
AcoI YGGCCR 1 cut(s) 340
AcsI RAATTY 5 cut(s) 837, 1277, 1592, 1877, 1975
AcuI CTGAAG 2 cut(s) 144, 1247
AfaI GTAC 8 cut(s) 118, 271, 535, 818, 855, 1260, 1402, 1936
AfeI AGCGCT 1 cut(s) 1481
AfiI CCNNNNNNNGG 9 cut(s) 113, 184, 584, 1195, 1529, 1961, 2107, 2179, 2214
AjnI CCWGG 7 cut(s) 177, 183, 656, 760, 1188, 1541, 1681
AleI CACNNNNGTG 1 cut(s) 616
AluBI AGCT 9 cut(s) 10, 308, 1220, 1328, 1559, 1661, 1923, 2057, 2065
AluI AGCT 9 cut(s) 10, 308, 1220, 1328, 1559, 1661, 1923, 2057, 2065
Alw21I GWGCWC 3 cut(s) 613, 1561, 1637
Alw26I GTCTC 2 cut(s) 371, 2028
Alw44I GTGCAC 1 cut(s) 1633
AlwI GGATC 2 cut(s) 1088, 1261
AlwNI CAGNNNCTG 1 cut(s) 2113
Ama87I CYCGRG 1 cut(s) 1598
Aor51HI AGCGCT 1 cut(s) 1481
AoxI GGCC 5 cut(s) 282, 340, 347, 959, 2025
ApaLI GTGCAC 1 cut(s) 1633
ApeKI GCWGC 9 cut(s) 110, 290, 305, 1546, 1724, 1829, 2057, 2123, 2153
ApoI RAATTY 5 cut(s) 837, 1277, 1592, 1877, 1975
AseI ATTAAT 2 cut(s) 1067, 2009
Asp700I GAANNNNTTC 4 cut(s) 147, 221, 1008, 2168
AspLEI GCGC 1 cut(s) 1482
AspS9I GGNCC 3 cut(s) 181, 575, 960
AsuC2I CCSGG 1 cut(s) 107
AsuHPI GGTGA 6 cut(s) 167, 272, 324, 775, 1328, 1472
AvaI CYCGRG 1 cut(s) 1598
AvaII GGWCC 2 cut(s) 181, 575
BaeGI GKGCMC 1 cut(s) 1637
BanII GRGCYC 3 cut(s) 518, 1561, 2185
Bbv12I GWGCWC 3 cut(s) 613, 1561, 1637
BbvI GCAGC 9 cut(s) 97, 277, 317, 1558, 1736, 1816, 2044, 2110, 2165
BceAI ACGGC 1 cut(s) 355
BciT130I CCWGG 7 cut(s) 179, 185, 658, 762, 1190, 1543, 1683
BciVI GTATCC 1 cut(s) 913
BclI TGATCA 1 cut(s) 628
BcnI CCSGG 1 cut(s) 107
BcoDI GTCTC 2 cut(s) 371, 2028
BfaI CTAG 3 cut(s) 519, 1203, 1817
BfmI CTRYAG 2 cut(s) 435, 1404
BfoI RGCGCY 1 cut(s) 1483
BfuI GTATCC 1 cut(s) 913
BglII AGATCT 1 cut(s) 1944
BisI GCNGC 9 cut(s) 111, 291, 306, 1547, 1725, 1830, 2058, 2124, 2154
BlsI GCNGC 9 cut(s) 112, 292, 307, 1548, 1726, 1831, 2059, 2125, 2155
Bme1390I CCNGG 8 cut(s) 107, 179, 185, 658, 762, 1190, 1543, 1683
Bme18I GGWCC 2 cut(s) 181, 575
BmeT110I CYCGRG 1 cut(s) 1598
BmgT120I GGNCC 3 cut(s) 181, 575, 960
BmrFI CCNGG 8 cut(s) 107, 179, 185, 658, 762, 1190, 1543, 1683
BmsI GCATC 7 cut(s) 424, 695, 1062, 1129, 1189, 1431, 1480
Bpu10I CCTNAGC 1 cut(s) 11
BpuMI CCSGG 1 cut(s) 107
Bsa29I ATCGAT 1 cut(s) 1704
BsaI GGTCTC 2 cut(s) 371, 2028
BsaJI CCNNGG 5 cut(s) 387, 657, 892, 1681, 1682
BsaXI ACNNNNNCTCC 2 cut(s) 1543, 1573
Bsc4I CCNNNNNNNGG 9 cut(s) 113, 184, 584, 1195, 1529, 1961, 2107, 2179, 2214
Bse118I RCCGGY 2 cut(s) 113, 362
Bse1I ACTGG 1 cut(s) 1586
BseBI CCWGG 7 cut(s) 179, 185, 658, 762, 1190, 1543, 1683
BseCI ATCGAT 1 cut(s) 1704
BseDI CCNNGG 5 cut(s) 387, 657, 892, 1681, 1682
BseGI GGATG 8 cut(s) 262, 493, 653, 710, 757, 1055, 1204, 1388
BseLI CCNNNNNNNGG 9 cut(s) 113, 184, 584, 1195, 1529, 1961, 2107, 2179, 2214
BseMII CTCAG 3 cut(s) 794, 1307, 1467
BseNI ACTGG 1 cut(s) 1586
BseRI GAGGAG 1 cut(s) 191
BseSI GKGCMC 1 cut(s) 1637
BseXI GCAGC 9 cut(s) 97, 277, 317, 1558, 1736, 1816, 2044, 2110, 2165
BsgI GTGCAG 1 cut(s) 2003
BshFI GGCC 5 cut(s) 284, 342, 349, 961, 2027
BshVI ATCGAT 1 cut(s) 1704
BsiHKAI GWGCWC 3 cut(s) 613, 1561, 1637
BsiHKCI CYCGRG 1 cut(s) 1598
BsiSI CCGG 3 cut(s) 107, 114, 363
BslI CCNNNNNNNGG 9 cut(s) 113, 184, 584, 1195, 1529, 1961, 2107, 2179, 2214
BsmAI GTCTC 2 cut(s) 371, 2028
BsmI GAATGC 2 cut(s) 216, 293
BsnI GGCC 5 cut(s) 284, 342, 349, 961, 2027
Bso31I GGTCTC 2 cut(s) 371, 2028
BsoBI CYCGRG 1 cut(s) 1598
Bsp1286I GDGCHC 5 cut(s) 518, 613, 1561, 1637, 2185
Bsp1407I TGTACA 4 cut(s) 269, 533, 816, 1934
Bsp143I GATC 5 cut(s) 628, 1093, 1253, 1375, 1944
Bsp19I CCATGG 1 cut(s) 892
BspACI CCGC 3 cut(s) 288, 398, 1055
BspANI GGCC 5 cut(s) 284, 342, 349, 961, 2027
BspCNI CTCAG 3 cut(s) 795, 1306, 1468
BspDI ATCGAT 1 cut(s) 1704
BspMAI CTGCAG 2 cut(s) 439, 1408
BspPI GGATC 2 cut(s) 1088, 1261
BspQI GCTCTTC 2 cut(s) 120, 993
BspTNI GGTCTC 2 cut(s) 371, 2028
BsrBI CCGCTC 1 cut(s) 400
BsrFI RCCGGY 2 cut(s) 113, 362
BsrGI TGTACA 4 cut(s) 269, 533, 816, 1934
BsrI ACTGG 1 cut(s) 1586
BssAI RCCGGY 2 cut(s) 113, 362
BssECI CCNNGG 5 cut(s) 387, 657, 892, 1681, 1682
BssMI GATC 5 cut(s) 628, 1093, 1253, 1375, 1944
BssT1I CCWWGG 2 cut(s) 387, 892
Bst2UI CCWGG 7 cut(s) 179, 185, 658, 762, 1190, 1543, 1683
Bst4CI ACNGT 5 cut(s) 50, 269, 473, 934, 1971
Bst6I CTCTTC 3 cut(s) 120, 951, 993
BstAPI GCANNNNNTGC 1 cut(s) 1199
BstAUI TGTACA 4 cut(s) 269, 533, 816, 1934
BstC8I GCNNGC 9 cut(s) 132, 190, 351, 557, 613, 959, 1077, 1081, 1227
BstDEI CTNAG 8 cut(s) 11, 155, 419, 458, 803, 1293, 1324, 1476
BstDSI CCRYGG 1 cut(s) 892
BstENI CCTNNNNNAGG 1 cut(s) 1959
BstF5I GGATG 8 cut(s) 262, 493, 653, 710, 757, 1055, 1204, 1388
BstH2I RGCGCY 1 cut(s) 1483
BstHHI GCGC 1 cut(s) 1482
BstKTI GATC 5 cut(s) 631, 1096, 1256, 1378, 1947
BstMAI GTCTC 2 cut(s) 371, 2028
BstMBI GATC 5 cut(s) 628, 1093, 1253, 1375, 1944
BstMWI GCNNNNNNNGC 6 cut(s) 127, 290, 359, 855, 1199, 1226
BstNI CCWGG 7 cut(s) 179, 185, 658, 762, 1190, 1543, 1683
BstNSI RCATGY 3 cut(s) 362, 559, 1731
BstSCI CCNGG 8 cut(s) 105, 177, 183, 656, 760, 1188, 1541, 1681
BstSFI CTRYAG 2 cut(s) 435, 1404
BstSLI GKGCMC 1 cut(s) 1637
BstV1I GCAGC 9 cut(s) 97, 277, 317, 1558, 1736, 1816, 2044, 2110, 2165
BstX2I RGATCY 3 cut(s) 1093, 1253, 1944
BstYI RGATCY 3 cut(s) 1093, 1253, 1944
Bsu15I ATCGAT 1 cut(s) 1704
BsuI GTATCC 1 cut(s) 913
BsuRI GGCC 5 cut(s) 284, 342, 349, 961, 2027
BsuTUI ATCGAT 1 cut(s) 1704
BtgI CCRYGG 1 cut(s) 892
BtsCI GGATG 8 cut(s) 262, 493, 653, 710, 757, 1055, 1204, 1388
BtsI GCAGTG 1 cut(s) 199
BtsIMutI CAGTG 2 cut(s) 199, 1239
Cac8I GCNNGC 9 cut(s) 132, 190, 351, 557, 613, 959, 1077, 1081, 1227
CaiI CAGNNNCTG 1 cut(s) 2113
CfoI GCGC 1 cut(s) 1482
Cfr10I RCCGGY 2 cut(s) 113, 362
Cfr13I GGNCC 3 cut(s) 181, 575, 960
ClaI ATCGAT 1 cut(s) 1704
CseI GACGC 2 cut(s) 548, 1456
CsiI ACCWGGT 1 cut(s) 177
Csp6I GTAC 8 cut(s) 117, 270, 534, 817, 854, 1259, 1401, 1935
CspCI CAANNNNNGTGG 4 cut(s) 472, 507, 1551, 1586
CviQI GTAC 8 cut(s) 117, 270, 534, 817, 854, 1259, 1401, 1935
DdeI CTNAG 8 cut(s) 11, 155, 419, 458, 803, 1293, 1324, 1476
DpnI GATC 5 cut(s) 630, 1095, 1255, 1377, 1946
DpnII GATC 5 cut(s) 628, 1093, 1253, 1375, 1944
DraI TTTAAA 1 cut(s) 591
EaeI YGGCCR 1 cut(s) 340
Eam1104I CTCTTC 3 cut(s) 120, 951, 993
EarI CTCTTC 3 cut(s) 120, 951, 993
Ecl136II GAGCTC 1 cut(s) 1559
Eco130I CCWWGG 2 cut(s) 387, 892
Eco24I GRGCYC 3 cut(s) 518, 1561, 2185
Eco31I GGTCTC 2 cut(s) 371, 2028
Eco47I GGWCC 2 cut(s) 181, 575
Eco47III AGCGCT 1 cut(s) 1481
Eco53kI GAGCTC 1 cut(s) 1559
Eco57I CTGAAG 2 cut(s) 144, 1247
Eco88I CYCGRG 1 cut(s) 1598
EcoICRI GAGCTC 1 cut(s) 1559
EcoNI CCTNNNNNAGG 1 cut(s) 1959
EcoO109I RGGNCCY 1 cut(s) 575
EcoRI GAATTC 1 cut(s) 1277
EcoRII CCWGG 7 cut(s) 177, 183, 656, 760, 1188, 1541, 1681
EcoT14I CCWWGG 2 cut(s) 387, 892
EcoT38I GRGCYC 3 cut(s) 518, 1561, 2185
ErhI CCWWGG 2 cut(s) 387, 892
FalI AAGNNNNNCTT 2 cut(s) 1209, 1241
FauNDI CATATG 2 cut(s) 274, 1145
FbaI TGATCA 1 cut(s) 628
FblI GTMKAC 1 cut(s) 562
Fnu4HI GCNGC 9 cut(s) 111, 291, 306, 1547, 1725, 1830, 2058, 2124, 2154
FokI GGATG 8 cut(s) 269, 500, 640, 717, 744, 1062, 1211, 1395
FriOI GRGCYC 3 cut(s) 518, 1561, 2185
Fsp4HI GCNGC 9 cut(s) 111, 291, 306, 1547, 1725, 1830, 2058, 2124, 2154
FspBI CTAG 3 cut(s) 519, 1203, 1817
GlaI GCGC 1 cut(s) 1481
GluI GCNGC 9 cut(s) 111, 291, 306, 1547, 1725, 1830, 2058, 2124, 2154
HaeII RGCGCY 1 cut(s) 1483
HaeIII GGCC 5 cut(s) 284, 342, 349, 961, 2027
HapII CCGG 3 cut(s) 107, 114, 363
HgaI GACGC 2 cut(s) 548, 1456
HhaI GCGC 1 cut(s) 1482
Hin6I GCGC 1 cut(s) 1480
HinP1I GCGC 1 cut(s) 1480
HindIII AAGCTT 3 cut(s) 8, 1218, 2063
HinfI GANTC 6 cut(s) 235, 622, 715, 1472, 1782, 1813
HpaII CCGG 3 cut(s) 107, 114, 363
HphI GGTGA 6 cut(s) 167, 272, 324, 775, 1328, 1472
Hpy166II GTNNAC 5 cut(s) 536, 563, 1463, 1635, 2021
Hpy188I TCNGA 9 cut(s) 480, 543, 927, 1098, 1266, 1369, 1396, 1477, 2189
Hpy188III TCNNGA 7 cut(s) 626, 992, 1598, 1600, 1669, 1958, 2215
Hpy8I GTNNAC 5 cut(s) 536, 563, 1463, 1635, 2021
Hpy99I CGWCG 1 cut(s) 1450
HpyCH4III ACNGT 5 cut(s) 50, 269, 473, 934, 1971
HpyF10VI GCNNNNNNNGC 6 cut(s) 127, 290, 359, 855, 1199, 1226
HpyF3I CTNAG 8 cut(s) 11, 155, 419, 458, 803, 1293, 1324, 1476
HspAI GCGC 1 cut(s) 1480
Ksp22I TGATCA 1 cut(s) 628
Kzo9I GATC 5 cut(s) 628, 1093, 1253, 1375, 1944
LguI GCTCTTC 2 cut(s) 120, 993
LmnI GCTCC 2 cut(s) 1556, 1564
Lsp1109I GCAGC 9 cut(s) 97, 277, 317, 1558, 1736, 1816, 2044, 2110, 2165
LweI GCATC 7 cut(s) 424, 695, 1062, 1129, 1189, 1431, 1480
MabI ACCWGGT 1 cut(s) 177
MaeI CTAG 3 cut(s) 519, 1203, 1817
MaeIII GTNAC 7 cut(s) 173, 194, 900, 1316, 1337, 1730, 2142
MalI GATC 5 cut(s) 630, 1095, 1255, 1377, 1946
MbiI CCGCTC 1 cut(s) 400
MboI GATC 5 cut(s) 628, 1093, 1253, 1375, 1944
MfeI CAATTG 3 cut(s) 1044, 1467, 2081
MflI RGATCY 3 cut(s) 1093, 1253, 1944
MhlI GDGCHC 5 cut(s) 518, 613, 1561, 1637, 2185
MlyI GAGTC 1 cut(s) 1466
MmeI TCCRAC 2 cut(s) 231, 1551
MroXI GAANNNNTTC 4 cut(s) 147, 221, 1008, 2168
MseI TTAA 5 cut(s) 590, 1067, 1113, 2009, 2073
MslI CAYNNNNRTG 4 cut(s) 616, 636, 864, 1704
MspA1I CMGCKG 1 cut(s) 290
MspI CCGG 3 cut(s) 107, 114, 363
MspR9I CCNGG 8 cut(s) 107, 179, 185, 658, 762, 1190, 1543, 1683
MssI GTTTAAAC 1 cut(s) 591
MunI CAATTG 3 cut(s) 1044, 1467, 2081
Mva1269I GAATGC 2 cut(s) 216, 293
MvaI CCWGG 7 cut(s) 179, 185, 658, 762, 1190, 1543, 1683
MwoI GCNNNNNNNGC 6 cut(s) 127, 290, 359, 855, 1199, 1226
NciI CCSGG 1 cut(s) 107
NcoI CCATGG 1 cut(s) 892
NdeI CATATG 2 cut(s) 274, 1145
NdeII GATC 5 cut(s) 628, 1093, 1253, 1375, 1944
NmeAIII GCCGAG 1 cut(s) 2053
NmuCI GTSAC 4 cut(s) 173, 194, 1316, 1337
NspI RCATGY 3 cut(s) 362, 559, 1731
OliI CACNNNNGTG 1 cut(s) 616
PaeI GCATGC 1 cut(s) 559
PaeR7I CTCGAG 1 cut(s) 1598
PasI CCCWGGG 1 cut(s) 1682
PciSI GCTCTTC 2 cut(s) 120, 993
PcsI WCGNNNNNNNCGW 1 cut(s) 1605
PctI GAATGC 2 cut(s) 216, 293
PdmI GAANNNNTTC 4 cut(s) 147, 221, 1008, 2168
PfeI GAWTC 5 cut(s) 235, 622, 715, 1782, 1813
PflFI GACNNNGTC 1 cut(s) 1363
PflMI CCANNNNNTGG 1 cut(s) 1529
PkrI GCNGC 9 cut(s) 112, 292, 307, 1548, 1726, 1831, 2059, 2125, 2155
PleI GAGTC 1 cut(s) 1466
PmeI GTTTAAAC 1 cut(s) 591
PpsI GAGTC 1 cut(s) 1466
PpuMI RGGWCCY 1 cut(s) 575
PshBI ATTAAT 2 cut(s) 1067, 2009
PsiI TTATAA 1 cut(s) 447
Psp124BI GAGCTC 1 cut(s) 1561
Psp5II RGGWCCY 1 cut(s) 575
Psp6I CCWGG 7 cut(s) 177, 183, 656, 760, 1188, 1541, 1681
PspGI CCWGG 7 cut(s) 177, 183, 656, 760, 1188, 1541, 1681
PspPI GGNCC 3 cut(s) 181, 575, 960
PspPPI RGGWCCY 1 cut(s) 575
PstI CTGCAG 2 cut(s) 439, 1408
PstNI CAGNNNCTG 1 cut(s) 2113
PsuI RGATCY 3 cut(s) 1093, 1253, 1944
PsyI GACNNNGTC 1 cut(s) 1363
RsaI GTAC 8 cut(s) 118, 271, 535, 818, 855, 1260, 1402, 1936
RsaNI GTAC 8 cut(s) 117, 270, 534, 817, 854, 1259, 1401, 1935
RseI CAYNNNNRTG 4 cut(s) 616, 636, 864, 1704
SacI GAGCTC 1 cut(s) 1561
SapI GCTCTTC 2 cut(s) 120, 993
SaqAI TTAA 5 cut(s) 590, 1067, 1113, 2009, 2073
SatI GCNGC 9 cut(s) 111, 291, 306, 1547, 1725, 1830, 2058, 2124, 2154
Sau3AI GATC 5 cut(s) 628, 1093, 1253, 1375, 1944
Sau96I GGNCC 3 cut(s) 181, 575, 960
SchI GAGTC 1 cut(s) 1466
ScrFI CCNGG 8 cut(s) 107, 179, 185, 658, 762, 1190, 1543, 1683
SduI GDGCHC 5 cut(s) 518, 613, 1561, 1637, 2185
SexAI ACCWGGT 1 cut(s) 177
SfaNI GCATC 7 cut(s) 424, 695, 1062, 1129, 1189, 1431, 1480
SfcI CTRYAG 2 cut(s) 435, 1404
Sfr274I CTCGAG 1 cut(s) 1598
SinI GGWCC 2 cut(s) 181, 575
SlaI CTCGAG 1 cut(s) 1598
SmiMI CAYNNNNRTG 4 cut(s) 616, 636, 864, 1704
SmlI CTYRAG 1 cut(s) 1598
SmoI CTYRAG 1 cut(s) 1598
SphI GCATGC 1 cut(s) 559
SsiI CCGC 3 cut(s) 288, 398, 1055
SspMI CTAG 3 cut(s) 519, 1203, 1817
SstI GAGCTC 1 cut(s) 1561
StyD4I CCNGG 8 cut(s) 105, 177, 183, 656, 760, 1188, 1541, 1681
StyI CCWWGG 2 cut(s) 387, 892
TaaI ACNGT 5 cut(s) 50, 269, 473, 934, 1971
TaqI TCGA 5 cut(s) 141, 151, 1033, 1599, 1704
TaqII GACCGA 1 cut(s) 1386
TatI WGTACW 6 cut(s) 269, 533, 816, 1258, 1400, 1934
TfiI GAWTC 5 cut(s) 235, 622, 715, 1782, 1813
Tru1I TTAA 5 cut(s) 590, 1067, 1113, 2009, 2073
Tru9I TTAA 5 cut(s) 590, 1067, 1113, 2009, 2073
TscAI CASTG 2 cut(s) 199, 1246
TseFI GTSAC 4 cut(s) 173, 194, 1316, 1337
TseI GCWGC 9 cut(s) 110, 290, 305, 1546, 1724, 1829, 2057, 2123, 2153
Tsp45I GTSAC 4 cut(s) 173, 194, 1316, 1337
TspGWI ACGGA 1 cut(s) 1325
TspRI CASTG 2 cut(s) 199, 1246
Tth111I GACNNNGTC 1 cut(s) 1363
Van91I CCANNNNNTGG 1 cut(s) 1529
VneI GTGCAC 1 cut(s) 1633
VpaK11BI GGWCC 2 cut(s) 181, 575
VspI ATTAAT 2 cut(s) 1067, 2009
XagI CCTNNNNNAGG 1 cut(s) 1959
XapI RAATTY 5 cut(s) 837, 1277, 1592, 1877, 1975
XceI RCATGY 3 cut(s) 362, 559, 1731
XhoI CTCGAG 1 cut(s) 1598
XmiI GTMKAC 1 cut(s) 562
XmnI GAANNNNTTC 4 cut(s) 147, 221, 1008, 2168
XspI CTAG 3 cut(s) 519, 1203, 1817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.