Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0000173

Physical Location & Seq

Genomic Coordinates Forward (+)

15406995 .. 15415268

Gene Structure 8274 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1158700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2283 bp

ATGTGGAAGCTTAAGGTCGCAGATGGTGGCAATGATCCATACATCTACAGCACAAACAACTTCGTGGGTAGGCAGATATTTGAGTTTGACCCTGAAGCAGGAACTACCGAACAGCGAGCTGAGGTGGAAGAAGCTCGTCTTCATTTCTACAATAATCGCTATCAGGTTAAGCCCAATAGTGACCTCCTATGGCGAATGCAGTTACTAAGGGAGAAGAACTTCAAACAGACAATTCCCCCGGTGAAAGTTGATGATGGAGAGGAAATCACATACGAAAAGGCCACCGTTACATTGAGAAGGTCTATCCACTTCTTTTCAGCTTTGCAGGCTAGTGATGGCCATTGGCCGGCTGAAAATGCCGGCGTGTTGTTTTTCCTTCCTCCTTTGGTCATGTGCACTTACATTACGGGACATCTTAACACTGTATTCTCTACAGAACACTGCAAAGAAATTTTGCGTTACATAAACTATCATCAGAACCAAGATGGTGGTTGGGGATTACACATTGAAGGACACAGCACCATGTTCTGCACAGCTCTCAACTACATTTGCATGCGTATTCTTGGAGAAGGCCCTGATGGTGGCCAAGACAATGCTTGTGCAAGAGCTAGAAAGTGGATTCTTGATCATGGCAGTGTTACCCACATCCCCTCTTGGGGTAAAACTTGGCTTTCGATACTTGGTGTGTTTGAGTGGTCCGGAAGCAACCCAATGCCCCCAGAGTTTTGGATTCTGCCTTCATTTCTTCCTATGCATCCAGCAAAAATGTGGTGCTACTGTAGGATGGTTTACATGCCTATGTCATATTTATATGGGAAAAGATTTGTTGGTCCAATAACATCTCTCACACTACAATTAAGGGAAGAACTCTATGCTCAACCTTATGACGAAATTAATTGGAAGGGAGTGCGTCATCACTGCGCTAAGGTGGACATCTACTACCCTCATCCTTGGATACAGGACTTTCTATGGGATTGTCTGTACGTATGTACGGAGCCTCTTCTTACTCGCTGGCCGTTTAACAAGCTGATCAGGAAAAGGGCTCTTGAAGTAACAATGGAGCATGTTCATTATGAAGATGAAAACAGCAGATACATTACCATTGGATGTGTGGAAAAGGTGTTATGCATGTTGGCTTGTTGGGTGGAAGATCCAAATGGGGATTATTTTAAAAAGCATCTTGCCAGGATACCGGATTATTTATGGGTTGCTGAGGATGGGATGAAGATGCATGGTTTTGGCAGTCAACTGTGGGATACTGGTCTTGCCATTCAAGCTTTGCTTGCTTCCAATCTAACCGATGAAATTGCACCCACTCTTGCTAGAGGACATGACTTCGTCAAGAAATCTCAGGTCAAGGACAATCCGTCGGGTGACTTCAAAAGCATGTACCGCCACATTTCCAAAGGATCGTGGACTTTCTCTGATCAAGATCATGGATGGCAAGTTTCTGATTGCACGGCAGAGGGTTTAAAGTGTTGCCTGCTTTTATCGATGATGCCACCAGAGATAGTTGGTGAAAAAATGGAGCCTGAGCGGTTATACGATGCTGTCAATGTCCTAATTTCCCTACAGAGTAAGAATGGTGGCTTAGCAGCCTGGGAACCAGCAGGAGCAGCAGAATGGTTAGAAATGTTAAATCCCACAGAATTCCTCGCGGACATTGTGGTTGAGCATGAATATGCTGAGTGCACTTCATCTGCAATCCAGGCTTTAGTTTTATTTAAGAAGTTATACCCTGGCCACAGGAAGGAAGAGATTGATCAATTTATTACCGCCGCTGCACTGTACCTTGAAAACGTACAAATGGCAGATGGTTCATGGTACGGGAGTTGGGGAGTTTGCTTCATATATGGTACCTGGTTTGCTCTTGGGGGGCTAACTGCTGCTGGTAAGACCTTCAACAATTGTGCAGCCATGCGCAAAGCTATTAGTTTTCTACTCGCAATCCAGAAAGAGAATGGTGGTTGGGGAGAGAGCTACCTTTCATGTCCAAAAAAGGAGTACGTTCCTCTTGAAGGAAACCGATCAAATTTAGTGCACACAGCTTGGGCTATGATGGGTTTGATTCATGCAGGGCAGGCGGAAAGAGACCCCACACCTCTTCATCGTGCAGCAAAGTTGATAATCAATTCTCAGATGGAAAATGGTGATTTTCCTCAACAGGAAATCACTGGAGTATTCTTGAAGAACTGCATGCTACATTATGCAGCTTATAGAAATATCTTTCCCATGTGGGCACTTGCAGAATACCGCAGGCGGGTTCCGTTCGTTGATGTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006629 GO:0006720 GO:0006721 GO:0006722 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009987 GO:0016104 GO:0016114 GO:0016853 GO:0016866 GO:0019742 GO:0019745 GO:0031559 GO:0042300 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0071704 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00909 ko01110 map00909 map01110

Pfam Domains

Protein Families

SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

761

Amino Acids

87.03

Weight (kDa)

5.94

Isoelectric Point (pI)

44.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SQHop_cyclase_N	PF13249	99 - 306	1.7e-34	Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C	PF13243	415 - 753	8e-52	Squalene-hopene cyclase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000173)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G66960 AT1G66960 AT1G66960 AT1G66960 AT1G78950 AT1G78950 AT1G78955 AT1G78955 AT1G78955 AT1G78955 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78970 AT1G78970 AT1G78970
fragaria_vesca	FvH4_5g29480 FvH4_6g36450 FvH4_6g36450 FvH4_6g36500 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g37620
malus_domestica	MD03G1089600.v1.1 MD09G1167700.v1.1 MD09G1168200.v1.1 MD09G1168300.v1.1 MD11G1098600.v1.1 MD17G1158300.v1.1 MD17G1158700.v1.1 MD17G1159500.v1.1 MD17G1160500.v1.1 MD17G1182500.v1.1 MD17G1182700.v1.1 MD17G1245800.v1.1 MD17G1246300.v1.1 MD17G1246600.v1.1
prunus_persica	Prupe.3G025700_v2.0.a1 Prupe.3G025700_v2.0.a1 Prupe.3G025800_v2.0.a1 Prupe.3G025900_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026400_v2.0.a1 Prupe.3G026500_v2.0.a1 Prupe.3G026700_v2.0.a1 Prupe.3G026800_v2.0.a1
pyrus_communis	pycom03g07120 pycom09g08520 pycom09g08590 pycom09g08610 pycom10g10070 pycom11g08310 pycom16g19880 pycom17g15190 pycom17g15300
rosa_chinensis	RchiOBHm_Chr2g0136221 RchiOBHm_Chr2g0148451 RchiOBHm_Chr2g0148501 RchiOBHm_Chr2g0148521 RchiOBHm_Chr2g0148661 RchiOBHm_Chr2g0148701 RchiOBHm_Chr2g0148811 RchiOBHm_Chr2g0148941 RchiOBHm_Chr2g0148981 RchiOBHm_Chr2g0150451 RchiOBHm_Chr2g0150521 RchiOBHm_Chr2g0150611 RchiOBHm_Chr4g0420831 RchiOBHm_Chr5g0049791 RchiOBHm_Chr5g0049801
rosa_laevigata	RLG00000007735 RLG00000020351 RLG00000020352 RLG00000020357 RLG00000020358 RLG00000020360 RLG00000029093 RLG00000030686
rosa_multiflora	Rmu_co8274513.1_g000001 Rmu_sc0000048.1_g000003 Rmu_sc0000048.1_g000033 Rmu_sc0000048.1_g000039 Rmu_sc0001397.1_g000011 Rmu_sc0001998.1_g000039 Rmu_sc0002070.1_g000055 Rmu_sc0002082.1_g000052 Rmu_sc0003181.1_g000010 Rmu_sc0005104.1_g000005 Rmu_sc0006758.1_g000002 Rmu_sc0008411.1_g000029 Rmu_sc0008411.1_g000030 Rmu_sc0017472.1_g000001 Rmu_sc0017472.1_g000003 Rmu_sc0029876.1_g000001 Rmu_ssc0000480.1_g000024 Rmu_ssc0000480.1_g000036
rosa_roxburghii	Rroxscaffold_2G00097070 Rroxscaffold_2G00098540 Rroxscaffold_2G00098560 Rroxscaffold_2G00098570 Rroxscaffold_2G00098650 Rroxscaffold_2G00098710 Rroxscaffold_2G00098740 Rroxscaffold_3G00225830 Rroxscaffold_3G00236870 Rroxscaffold_4G00312240
rosa_rugosa	Rorug01G0047000 Rorug02G0372300 Rorug02G0407200 Rorug02G0407300 Rorug02G0408400 Rorug02G0421500 Rorug07G0208900
rosa_samantha	Rh2AG380200 Rh2AG465700 Rh2AG465800 Rh2AG466800 Rh2AG467100 Rh2AG467600 Rh2AG480900 Rh2AG481400 Rh2AG481700 Rh2CG073500 Rh2CG452200 Rh2CG453200 Rh2CG453600 Rh2CG454100 Rh2CG467100 Rh2CG467800 Rh2CG468000 Rh2DG403100 Rh2DG487800 Rh2DG487900 Rh2DG488800 Rh2DG502000 Rh2DG502200 Rh2DG502300 Rh2DG503100 Rh3AG319300 Rh4AG227700 Rh4BG371800 Rh5AG327400 Rh5DG350600 Rh7AG349800
rosa_wichuraiana	Rw0G010080 Rw2G031110 Rw2G037980 Rw2G037990 Rw2G038000 Rw2G038010 Rw2G038080 Rw2G038100 Rw2G039260 Rw2G039290 Rw2G039470 Rw4G019710 Rw5G030930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1922
Acc65I	GGTACC	1 cut(s)	1856
AccB1I	GGYRCC	1 cut(s)	1856
AccBSI	CCGCTC	1 cut(s)	1537
AccII	CGCG	1 cut(s)	1658
AccIII	TCCGGA	1 cut(s)	698
AciI	CCGC	8 cut(s)	1393, 1537, 1658, 1776, 1779, 2084, 2254, 2260
AclWI	GGATC	3 cut(s)	29, 1145, 1417
AcoI	YGGCCR	5 cut(s)	337, 344, 583, 1013, 1741
AcsI	RAATTY	3 cut(s)	450, 1649, 2032
AcuI	CTGAAG	1 cut(s)	114
AfaI	GTAC	8 cut(s)	983, 991, 1391, 1790, 1803, 1826, 1858, 2006
AfiI	CCNNNNNNNGG	8 cut(s)	98, 346, 581, 655, 656, 1750, 2018, 2260
AflII	CTTAAG	1 cut(s)	11
AgsI	TTSAA	9 cut(s)	223, 509, 1049, 1274, 1381, 1796, 1903, 2018, 2188
AhdI	GACNNNNNGTC	1 cut(s)	1366
AjnI	CCWGG	5 cut(s)	1184, 1598, 1707, 1738, 1859
Alw21I	GWGCWC	3 cut(s)	398, 1694, 2043
Alw26I	GTCTC	1 cut(s)	2085
Alw44I	GTGCAC	3 cut(s)	394, 1690, 2039
AlwI	GGATC	3 cut(s)	29, 1145, 1417
Aor13HI	TCCGGA	1 cut(s)	698
AoxI	GGCC	7 cut(s)	279, 337, 344, 571, 583, 1013, 1741
ApaLI	GTGCAC	3 cut(s)	394, 1690, 2039
ApeKI	GCWGC	7 cut(s)	1595, 1616, 1781, 1886, 1913, 2114, 2210
ApoI	RAATTY	3 cut(s)	450, 1649, 2032
AseI	ATTAAT	1 cut(s)	894
Asp700I	GAANNNNTTC	1 cut(s)	218
Asp718I	GGTACC	1 cut(s)	1856
AspLEI	GCGC	2 cut(s)	923, 1923
AspS9I	GGNCC	3 cut(s)	572, 696, 830
AsuC2I	CCSGG	1 cut(s)	239
AsuHPI	GGTGA	4 cut(s)	253, 1385, 1529, 2162
AvaII	GGWCC	2 cut(s)	696, 830
BaeGI	GKGCMC	4 cut(s)	398, 1694, 2043, 2242
BaeI	ACNNNNGTAYC	2 cut(s)	1816, 1849
BalI	TGGCCA	3 cut(s)	339, 585, 1743
BanI	GGYRCC	1 cut(s)	1856
BanII	GRGCYC	1 cut(s)	1045
BbsI	GAAGAC	1 cut(s)	131
Bbv12I	GWGCWC	3 cut(s)	398, 1694, 2043
BbvCI	CCTCAGC	2 cut(s)	120, 1212
BbvI	GCAGC	7 cut(s)	1607, 1628, 1768, 1873, 1925, 2126, 2222
BceAI	ACGGC	2 cut(s)	1000, 1476
BcgI	CGANNNNNNTGC	2 cut(s)	1289, 1323
BciT130I	CCWGG	5 cut(s)	1186, 1600, 1709, 1740, 1861
BciVI	GTATCC	3 cut(s)	948, 1182, 1249
BclI	TGATCA	4 cut(s)	625, 1029, 1426, 1762
BcnI	CCSGG	1 cut(s)	239
BcoDI	GTCTC	1 cut(s)	2085
BfaI	CTAG	3 cut(s)	330, 609, 1323
BfmI	CTRYAG	4 cut(s)	46, 432, 778, 1571
BfrI	CTTAAG	1 cut(s)	11
BfuI	GTATCC	3 cut(s)	948, 1182, 1249
BisI	GCNGC	8 cut(s)	1596, 1617, 1779, 1782, 1887, 1914, 2115, 2211
BlpI	GCTNAGC	1 cut(s)	1591
BlsI	GCNGC	8 cut(s)	1597, 1618, 1780, 1783, 1888, 1915, 2116, 2212
Bme1390I	CCNGG	6 cut(s)	239, 1186, 1600, 1709, 1740, 1861
Bme18I	GGWCC	2 cut(s)	696, 830
BmeRI	GACNNNNNGTC	1 cut(s)	1366
BmgT120I	GGNCC	3 cut(s)	572, 696, 830
BmiI	GGNNCC	5 cut(s)	996, 1530, 1605, 1858, 2265
BmrFI	CCNGG	6 cut(s)	239, 1186, 1600, 1709, 1740, 1861
BmsI	GCATC	5 cut(s)	763, 1186, 1218, 1488, 1537
BpiI	GAAGAC	1 cut(s)	131
BpmI	CTGGAG	1 cut(s)	2196
Bpu10I	CCTNAGC	4 cut(s)	120, 924, 1212, 1533
Bpu1102I	GCTNAGC	1 cut(s)	1591
BpuMI	CCSGG	1 cut(s)	239
Bsa29I	ATCGAT	1 cut(s)	1493
BsaAI	YACGTR	1 cut(s)	985
BsaBI	GATNNNNATC	1 cut(s)	1431
BsaI	GGTCTC	1 cut(s)	2085
BsaJI	CCNNGG	4 cut(s)	237, 950, 1599, 1738
BsaWI	WCCGGW	2 cut(s)	698, 1192
Bsc4I	CCNNNNNNNGG	8 cut(s)	98, 346, 581, 655, 656, 1750, 2018, 2260
Bse118I	RCCGGY	2 cut(s)	346, 359
Bse1I	ACTGG	2 cut(s)	1264, 2179
Bse3DI	GCAATG	1 cut(s)	37
Bse8I	GATNNNNATC	1 cut(s)	1431
BseAI	TCCGGA	1 cut(s)	698
BseBI	CCWGG	5 cut(s)	1186, 1600, 1709, 1740, 1861
BseCI	ATCGAT	1 cut(s)	1493
BseDI	CCNNGG	4 cut(s)	237, 950, 1599, 1738
BseGI	GGATG	8 cut(s)	645, 754, 789, 946, 1112, 1222, 1227, 1445
BseJI	GATNNNNATC	1 cut(s)	1431
BseLI	CCNNNNNNNGG	8 cut(s)	98, 346, 581, 655, 656, 1750, 2018, 2260
BseMI	GCAATG	1 cut(s)	37
BseMII	CTCAG	6 cut(s)	111, 1203, 1364, 1524, 1677, 2150
BseNI	ACTGG	2 cut(s)	1264, 2179
BseSI	GKGCMC	4 cut(s)	398, 1694, 2043, 2242
BseXI	GCAGC	7 cut(s)	1607, 1628, 1768, 1873, 1925, 2126, 2222
BsgI	GTGCAG	4 cut(s)	514, 1767, 1932, 2133
Bsh1236I	CGCG	1 cut(s)	1658
BshFI	GGCC	7 cut(s)	281, 339, 346, 573, 585, 1015, 1743
BshNI	GGYRCC	1 cut(s)	1856
BshVI	ATCGAT	1 cut(s)	1493
BsiHKAI	GWGCWC	3 cut(s)	398, 1694, 2043
BsiSI	CCGG	5 cut(s)	239, 347, 360, 699, 1193
BslFI	GGGAC	1 cut(s)	423
BslI	CCNNNNNNNGG	8 cut(s)	98, 346, 581, 655, 656, 1750, 2018, 2260
BsmAI	GTCTC	1 cut(s)	2085
BsmFI	GGGAC	1 cut(s)	423
BsmI	GAATGC	1 cut(s)	201
BsnI	GGCC	7 cut(s)	281, 339, 346, 573, 585, 1015, 1743
Bso31I	GGTCTC	1 cut(s)	2085
Bsp1286I	GDGCHC	5 cut(s)	398, 1045, 1694, 2043, 2242
Bsp13I	TCCGGA	1 cut(s)	698
Bsp143I	GATC	9 cut(s)	34, 625, 1029, 1150, 1409, 1426, 1432, 1762, 2027
Bsp1720I	GCTNAGC	1 cut(s)	1591
BspACI	CCGC	8 cut(s)	1393, 1537, 1658, 1776, 1779, 2084, 2254, 2260
BspANI	GGCC	7 cut(s)	281, 339, 346, 573, 585, 1015, 1743
BspCNI	CTCAG	6 cut(s)	112, 1204, 1363, 1525, 1678, 2149
BspDI	ATCGAT	1 cut(s)	1493
BspEI	TCCGGA	1 cut(s)	698
BspFNI	CGCG	1 cut(s)	1658
BspLI	GGNNCC	5 cut(s)	996, 1530, 1605, 1858, 2265
BspPI	GGATC	3 cut(s)	29, 1145, 1417
BspT107I	GGYRCC	1 cut(s)	1856
BspTI	CTTAAG	1 cut(s)	11
BspTNI	GGTCTC	1 cut(s)	2085
BsrBI	CCGCTC	1 cut(s)	1537
BsrDI	GCAATG	1 cut(s)	37
BsrFI	RCCGGY	2 cut(s)	346, 359
BsrI	ACTGG	2 cut(s)	1264, 2179
BssAI	RCCGGY	2 cut(s)	346, 359
BssECI	CCNNGG	4 cut(s)	237, 950, 1599, 1738
BssMI	GATC	9 cut(s)	34, 625, 1029, 1150, 1409, 1426, 1432, 1762, 2027
BssT1I	CCWWGG	1 cut(s)	950
Bst2UI	CCWGG	5 cut(s)	1186, 1600, 1709, 1740, 1861
Bst4CI	ACNGT	5 cut(s)	286, 424, 779, 1251, 1788
Bst6I	CTCTTC	3 cut(s)	1005, 1749, 2109
BstAFI	CTTAAG	1 cut(s)	11
BstBAI	YACGTR	1 cut(s)	985
BstDEI	CTNAG	9 cut(s)	120, 206, 924, 1212, 1350, 1533, 1591, 1686, 2136
BstENI	CCTNNNNNAGG	2 cut(s)	96, 2016
BstF5I	GGATG	8 cut(s)	645, 754, 789, 946, 1112, 1222, 1227, 1445
BstFNI	CGCG	1 cut(s)	1658
BstHHI	GCGC	2 cut(s)	923, 1923
BstKTI	GATC	9 cut(s)	37, 628, 1032, 1153, 1412, 1429, 1435, 1765, 2030
BstMAI	GTCTC	1 cut(s)	2085
BstMBI	GATC	9 cut(s)	34, 625, 1029, 1150, 1409, 1426, 1432, 1762, 2027
BstMWI	GCNNNNNNNGC	8 cut(s)	326, 356, 1274, 1283, 1392, 1616, 1709, 2081
BstNI	CCWGG	5 cut(s)	1186, 1600, 1709, 1740, 1861
BstNSI	RCATGY	6 cut(s)	556, 796, 1067, 1132, 1390, 2200
BstSCI	CCNGG	6 cut(s)	237, 1184, 1598, 1707, 1738, 1859
BstSFI	CTRYAG	4 cut(s)	46, 432, 778, 1571
BstSLI	GKGCMC	4 cut(s)	398, 1694, 2043, 2242
BstSNI	TACGTA	1 cut(s)	985
BstUI	CGCG	1 cut(s)	1658
BstV1I	GCAGC	7 cut(s)	1607, 1628, 1768, 1873, 1925, 2126, 2222
BstV2I	GAAGAC	1 cut(s)	131
BstX2I	RGATCY	1 cut(s)	1150
BstXI	CCANNNNNNTGG	2 cut(s)	488, 726
BstYI	RGATCY	1 cut(s)	1150
Bsu15I	ATCGAT	1 cut(s)	1493
BsuI	GTATCC	3 cut(s)	948, 1182, 1249
BsuRI	GGCC	7 cut(s)	281, 339, 346, 573, 585, 1015, 1743
BsuTUI	ATCGAT	1 cut(s)	1493
BtsCI	GGATG	8 cut(s)	645, 754, 789, 946, 1112, 1222, 1227, 1445
BtsI	GCAGTG	3 cut(s)	439, 640, 916
BtsIMutI	CAGTG	6 cut(s)	420, 439, 640, 916, 1784, 2172
CfoI	GCGC	2 cut(s)	923, 1923
Cfr10I	RCCGGY	2 cut(s)	346, 359
Cfr13I	GGNCC	3 cut(s)	572, 696, 830
ClaI	ATCGAT	1 cut(s)	1493
CseI	GACGC	1 cut(s)	899
CsiI	ACCWGGT	1 cut(s)	1859
Csp6I	GTAC	8 cut(s)	982, 990, 1390, 1789, 1802, 1825, 1857, 2005
CviQI	GTAC	8 cut(s)	982, 990, 1390, 1789, 1802, 1825, 1857, 2005
DdeI	CTNAG	9 cut(s)	120, 206, 924, 1212, 1350, 1533, 1591, 1686, 2136
DpnI	GATC	9 cut(s)	36, 627, 1031, 1152, 1411, 1428, 1434, 1764, 2029
DpnII	GATC	9 cut(s)	34, 625, 1029, 1150, 1409, 1426, 1432, 1762, 2027
DraI	TTTAAA	2 cut(s)	1171, 1473
DriI	GACNNNNNGTC	1 cut(s)	1366
EaeI	YGGCCR	5 cut(s)	337, 344, 583, 1013, 1741
Eam1104I	CTCTTC	3 cut(s)	1005, 1749, 2109
Eam1105I	GACNNNNNGTC	1 cut(s)	1366
EarI	CTCTTC	3 cut(s)	1005, 1749, 2109
EciI	GGCGGA	1 cut(s)	2099
Eco105I	TACGTA	1 cut(s)	985
Eco130I	CCWWGG	1 cut(s)	950
Eco24I	GRGCYC	1 cut(s)	1045
Eco31I	GGTCTC	1 cut(s)	2085
Eco47I	GGWCC	2 cut(s)	696, 830
Eco57I	CTGAAG	1 cut(s)	114
EcoNI	CCTNNNNNAGG	2 cut(s)	96, 2016
EcoO109I	RGGNCCY	1 cut(s)	572
EcoRI	GAATTC	1 cut(s)	1649
EcoRII	CCWGG	5 cut(s)	1184, 1598, 1707, 1738, 1859
EcoT14I	CCWWGG	1 cut(s)	950
EcoT22I	ATGCAT	3 cut(s)	756, 1130, 1233
EcoT38I	GRGCYC	1 cut(s)	1045
ErhI	CCWWGG	1 cut(s)	950
FalI	AAGNNNNNCTT	4 cut(s)	123, 155, 1266, 1298
FaqI	GGGAC	1 cut(s)	423
FauI	CCCGC	1 cut(s)	2253
FbaI	TGATCA	4 cut(s)	625, 1029, 1426, 1762
Fnu4HI	GCNGC	8 cut(s)	1596, 1617, 1779, 1782, 1887, 1914, 2115, 2211
FokI	GGATG	8 cut(s)	632, 741, 796, 933, 1119, 1229, 1234, 1452
FriOI	GRGCYC	1 cut(s)	1045
Fsp4HI	GCNGC	8 cut(s)	1596, 1617, 1779, 1782, 1887, 1914, 2115, 2211
FspBI	CTAG	3 cut(s)	330, 609, 1323
FspI	TGCGCA	1 cut(s)	1922
GlaI	GCGC	2 cut(s)	922, 1922
GluI	GCNGC	8 cut(s)	1596, 1617, 1779, 1782, 1887, 1914, 2115, 2211
GsuI	CTGGAG	1 cut(s)	2196
HaeIII	GGCC	7 cut(s)	281, 339, 346, 573, 585, 1015, 1743
HapII	CCGG	5 cut(s)	239, 347, 360, 699, 1193
HgaI	GACGC	1 cut(s)	899
HhaI	GCGC	2 cut(s)	923, 1923
Hin6I	GCGC	2 cut(s)	921, 1921
HinP1I	GCGC	2 cut(s)	921, 1921
HincII	GTYRAC	1 cut(s)	1247
HindII	GTYRAC	1 cut(s)	1247
HindIII	AAGCTT	2 cut(s)	8, 1275
HinfI	GANTC	3 cut(s)	619, 730, 2068
HpaII	CCGG	5 cut(s)	239, 347, 360, 699, 1193
HphI	GGTGA	4 cut(s)	253, 1385, 1529, 2162
Hpy166II	GTNNAC	7 cut(s)	396, 790, 931, 1247, 1416, 1692, 2041
Hpy188I	TCNGA	4 cut(s)	477, 1426, 1453, 2139
Hpy188III	TCNNGA	9 cut(s)	623, 699, 1033, 1046, 1342, 1430, 1951, 2015, 2185
Hpy8I	GTNNAC	7 cut(s)	396, 790, 931, 1247, 1416, 1692, 2041
Hpy99I	CGWCG	1 cut(s)	1372
HpyAV	CCTTC	9 cut(s)	291, 386, 503, 563, 747, 895, 1744, 1909, 2012
HpyCH4III	ACNGT	5 cut(s)	286, 424, 779, 1251, 1788
HpyCH4IV	ACGT	3 cut(s)	984, 1800, 2007
HpyF10VI	GCNNNNNNNGC	8 cut(s)	326, 356, 1274, 1283, 1392, 1616, 1709, 2081
HpyF3I	CTNAG	9 cut(s)	120, 206, 924, 1212, 1350, 1533, 1591, 1686, 2136
HpySE526I	ACGT	3 cut(s)	984, 1800, 2007
HspAI	GCGC	2 cut(s)	921, 1921
Kpn2I	TCCGGA	1 cut(s)	698
KpnI	GGTACC	1 cut(s)	1860
KroI	GCCGGC	2 cut(s)	346, 359
KroNI	GCCGGC	2 cut(s)	348, 361
Ksp22I	TGATCA	4 cut(s)	625, 1029, 1426, 1762
Kzo9I	GATC	9 cut(s)	34, 625, 1029, 1150, 1409, 1426, 1432, 1762, 2027
LmnI	GCTCC	4 cut(s)	994, 1060, 1528, 1613
Lsp1109I	GCAGC	7 cut(s)	1607, 1628, 1768, 1873, 1925, 2126, 2222
LweI	GCATC	5 cut(s)	763, 1186, 1218, 1488, 1537
MabI	ACCWGGT	1 cut(s)	1859
MaeI	CTAG	3 cut(s)	330, 609, 1323
MaeII	ACGT	3 cut(s)	984, 1800, 2007
MaeIII	GTNAC	7 cut(s)	179, 201, 286, 458, 637, 1051, 1373
MalI	GATC	9 cut(s)	36, 627, 1031, 1152, 1411, 1428, 1434, 1764, 2029
MbiI	CCGCTC	1 cut(s)	1537
MboI	GATC	9 cut(s)	34, 625, 1029, 1150, 1409, 1426, 1432, 1762, 2027
MfeI	CAATTG	1 cut(s)	1906
MflI	RGATCY	1 cut(s)	1150
MhlI	GDGCHC	5 cut(s)	398, 1045, 1694, 2043, 2242
MlsI	TGGCCA	3 cut(s)	339, 585, 1743
MluNI	TGGCCA	3 cut(s)	339, 585, 1743
Mox20I	TGGCCA	3 cut(s)	339, 585, 1743
Mph1103I	ATGCAT	3 cut(s)	756, 1130, 1233
MroI	TCCGGA	1 cut(s)	698
MroNI	GCCGGC	2 cut(s)	346, 359
MroXI	GAANNNNTTC	1 cut(s)	218
MscI	TGGCCA	3 cut(s)	339, 585, 1743
MslI	CAYNNNNRTG	4 cut(s)	551, 633, 797, 1680
Msp20I	TGGCCA	3 cut(s)	339, 585, 1743
MspA1I	CMGCKG	1 cut(s)	1781
MspCI	CTTAAG	1 cut(s)	11
MspI	CCGG	5 cut(s)	239, 347, 360, 699, 1193
MspR9I	CCNGG	6 cut(s)	239, 1186, 1600, 1709, 1740, 1861
MunI	CAATTG	1 cut(s)	1906
Mva1269I	GAATGC	1 cut(s)	201
MvaI	CCWGG	5 cut(s)	1186, 1600, 1709, 1740, 1861
MvnI	CGCG	1 cut(s)	1658
MwoI	GCNNNNNNNGC	8 cut(s)	326, 356, 1274, 1283, 1392, 1616, 1709, 2081
NaeI	GCCGGC	2 cut(s)	348, 361
NciI	CCSGG	1 cut(s)	239
NdeII	GATC	9 cut(s)	34, 625, 1029, 1150, 1409, 1426, 1432, 1762, 2027
NgoMIV	GCCGGC	2 cut(s)	346, 359
NlaIV	GGNNCC	5 cut(s)	996, 1530, 1605, 1858, 2265
NmuCI	GTSAC	2 cut(s)	179, 1373
NsbI	TGCGCA	1 cut(s)	1922
NsiI	ATGCAT	3 cut(s)	756, 1130, 1233
NspI	RCATGY	6 cut(s)	556, 796, 1067, 1132, 1390, 2200
PaeI	GCATGC	2 cut(s)	556, 2200
PctI	GAATGC	1 cut(s)	201
PdiI	GCCGGC	2 cut(s)	348, 361
PdmI	GAANNNNTTC	1 cut(s)	218
PfeI	GAWTC	3 cut(s)	619, 730, 2068
PflFI	GACNNNGTC	1 cut(s)	1337
PkrI	GCNGC	8 cut(s)	1597, 1618, 1780, 1783, 1888, 1915, 2116, 2212
Ppu21I	YACGTR	1 cut(s)	985
PshBI	ATTAAT	1 cut(s)	894
Psp6I	CCWGG	5 cut(s)	1184, 1598, 1707, 1738, 1859
PspGI	CCWGG	5 cut(s)	1184, 1598, 1707, 1738, 1859
PspN4I	GGNNCC	5 cut(s)	996, 1530, 1605, 1858, 2265
PspPI	GGNCC	3 cut(s)	572, 696, 830
PsuI	RGATCY	1 cut(s)	1150
PsyI	GACNNNGTC	1 cut(s)	1337
RsaI	GTAC	8 cut(s)	983, 991, 1391, 1790, 1803, 1826, 1858, 2006
RsaNI	GTAC	8 cut(s)	982, 990, 1390, 1789, 1802, 1825, 1857, 2005
RseI	CAYNNNNRTG	4 cut(s)	551, 633, 797, 1680
SatI	GCNGC	8 cut(s)	1596, 1617, 1779, 1782, 1887, 1914, 2115, 2211
Sau3AI	GATC	9 cut(s)	34, 625, 1029, 1150, 1409, 1426, 1432, 1762, 2027
Sau96I	GGNCC	3 cut(s)	572, 696, 830
ScrFI	CCNGG	6 cut(s)	239, 1186, 1600, 1709, 1740, 1861
SduI	GDGCHC	5 cut(s)	398, 1045, 1694, 2043, 2242
SexAI	ACCWGGT	1 cut(s)	1859
SfaNI	GCATC	5 cut(s)	763, 1186, 1218, 1488, 1537
SfcI	CTRYAG	4 cut(s)	46, 432, 778, 1571
SinI	GGWCC	2 cut(s)	696, 830
SmiMI	CAYNNNNRTG	4 cut(s)	551, 633, 797, 1680
SmlI	CTYRAG	1 cut(s)	11
SmoI	CTYRAG	1 cut(s)	11
SnaBI	TACGTA	1 cut(s)	985
SphI	GCATGC	2 cut(s)	556, 2200
SsiI	CCGC	8 cut(s)	1393, 1537, 1658, 1776, 1779, 2084, 2254, 2260
SspMI	CTAG	3 cut(s)	330, 609, 1323
StyD4I	CCNGG	6 cut(s)	237, 1184, 1598, 1707, 1738, 1859
StyI	CCWWGG	1 cut(s)	950
TaaI	ACNGT	5 cut(s)	286, 424, 779, 1251, 1788
TaiI	ACGT	3 cut(s)	987, 1803, 2010
TaqI	TCGA	2 cut(s)	674, 1493
TauI	GCSGC	1 cut(s)	1781
TfiI	GAWTC	3 cut(s)	619, 730, 2068
TscAI	CASTG	6 cut(s)	427, 446, 640, 923, 1791, 2179
TseFI	GTSAC	2 cut(s)	179, 1373
TseI	GCWGC	7 cut(s)	1595, 1616, 1781, 1886, 1913, 2114, 2210
Tsp45I	GTSAC	2 cut(s)	179, 1373
TspGWI	ACGGA	3 cut(s)	1007, 1356, 2256
TspRI	CASTG	6 cut(s)	427, 446, 640, 923, 1791, 2179
Tth111I	GACNNNGTC	1 cut(s)	1337
Vha464I	CTTAAG	1 cut(s)	11
VneI	GTGCAC	3 cut(s)	394, 1690, 2039
VpaK11BI	GGWCC	2 cut(s)	696, 830
VspI	ATTAAT	1 cut(s)	894
XagI	CCTNNNNNAGG	2 cut(s)	96, 2016
XapI	RAATTY	3 cut(s)	450, 1649, 2032
XceI	RCATGY	6 cut(s)	556, 796, 1067, 1132, 1390, 2200
XcmI	CCANNNNNNNNNTGG	3 cut(s)	765, 1108, 1958
XmnI	GAANNNNTTC	1 cut(s)	218
XspI	CTAG	3 cut(s)	330, 609, 1323
Zsp2I	ATGCAT	3 cut(s)	756, 1130, 1233

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.