MD09G1167700.v1.1

Belongs to the terpene cyclase mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000173
Physical Location & Seq
Reverse (-)
13752030 .. 13757131
5102 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1167700.v1.1.491

Sequence Viewer

Length: 2283 bp
ATGTGGAAGATCAAGTTTGGAGAGGGTGCAAATGACCCTATGTTGTTCAGCACAAACAACTTCCATGGAAGGCAGACATGGGAGTTCGACCCAGATGCAGGTACCGAGGAGGAGCGAGCAGAAGTTGAAGCTGCTCGTGAGCATTTCTACCAAAATCGCTTCAAGGTCCAGCCTAGCAGCGACCTCCTATGGCGTTTTCAGATACTAAGAGAGAAAAACTTTAAACAAGAAATTCCTCCAGTAAGAGTTGGGGAGGGTGAGGACATTACATATGATCAGGCCACAGCTGCATTCAGGAGGGCTGCTACCTTCTGGAATGCCTTGCAATCACCCCATGGACATTGGCCTGCTGAAAATGCTGGCCCAAACTTTTACTTCCCTCCCTTGGTCATGGCTGCATACATTCCAGGATATCTCAATGTTATTTTCTCCGCTGAGCACAAGAAGGAAATCTTGCGTTACACATACAACCATCAAAATGAAGATGGTGGCTGGGGACTGCACATAGCGGGGCCTAGTATGATGTTTACTACATGCCTAAACTACTGTATGATGCGTATTCTCGGAGATGGCCCCGATGGTGGTCGTGACAATGCCTGCGCAAGGGCACGAAAGTGGATTCTTGACCGTGGTGGTGCGTACTATTCTGCATCATGGGGCAAAACTTGGATGGCGATTCTTGGTGTGTATGACTGGGAAGGCAGCAACCCAATGCCCCCAGAATTCTGGACTGGGTCGACTCTGCTTCCTTTTCATCCATCAAAAATGTTCTGCTACTGTAGGTTGACTTACCTACCTATGTCTTACTTTTATGCCACAAGATTTGTTGGCCCAATCACTCCACTTGTTGAGGAATTGAGACAAGAAATTTACTGTGAATCTTACAACGAAATTAACTGGCCTAAAGTGCGCCATTGGTGTGCAACAGAAGATAACTACTATCCCCATGGTCGTGTACAACGTTTTATGTGGGACGGTTTTTACAATATCGTCGAGCCTCTCCTAAAACGCTGGCCTTTCAAGAAGATCAGAGACAATGCAATTCAATTCACAATTGACCAAATTCATTATGAAGATGAGAACAGTCGTTACATTACAATCGGATGCGTGGAAAAGCCATTGATGATGCTTGCTTGCTGGGCCGAGGATCCTAGTGGAGAAGCTTTCAAGAAGCATCTTCCTAGAGTTACCGATTATATCTGGCTCGGAGAGGATGGAATCAAGATGCAAAGTTTTGGAAGCCAGTCATGGGATTGTGCTCTTGTGATTCAAGCTTTGCTTGCTGGGAATCTTAATGCTGAAATGGGACCCACCCTTAAGAAAGCACACGAATTCCTCAAGATATCTCAGGTGAGGATTAATACATCCGGTGACTACCTATCTCATTTCCGTCACATTTCAAAGGGCGCATGGACATTCTCTGACCGTGATCATGGATGGCAAGTGTCAGATTGCACTGCAGAAGCACTGAGGTGTTGCTGCATTTTTGCAAATATGTCCCCAGAAGTTGTTGGTGAGCCAATGGAAGCTGAGTGTATGTATGATGCTGTCAATGTCATCATGTCTCTTCAAAGTCCAAATGGTGGTGTATCAGCCTGGGAGCCAACAGGAGCACCAAAATGGTTGGAGTGGCTCAACCCTGTGGAGTTTCTTGAGGACCTTGTCATTGAGTACGAGTACATCGAGTGCACTTCATCTTCGATCCAGGCCTTAACATTGTTTAGGAAGTTGTACCCTGGCCATAGAAGGAAGGAGATCAACAATTTCATCACAAGGGCTGCAGACTACATTGAAGACATACAGTACCCTGATGGCTCATGGTATGGAAACTGGGGAATCTGCTTCGTGTATGGTACATGGTTCGCAATCAAAGGGCTGGAGGCTGCCGGCAGGACATACAACAACTGTGAGGCAGTGCGCAAAGGTGTCGACTTTTTGCTCAAAACACAAAGGGCAGATGGTGGCTGGGGAGAGCACTACACCTCATGCACAAACAAGAAATATACAGCTCAAGACAGTACAAATTTAGTCCAAACTGCACTCGGATTAATGGGTCTGATTCACGGTCGACAGGCTGAGAGAGATCCAACTCCTATTCACCGGGCTGCTGCGGTGTTAATGAACGGTCAATTGGATGACGGCGATTTCCCCCAACAGGAACTGATGGGAGTTTTTATGAGGAATGCTATGTTGCACTATGCAGCATACAGAAATATCTTCCCATTGTGGGCTCTCGGTGAATACCGCACACTGGTTTCGTTGCCTATCAAAAAGATCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 ko01110 map00909 map01110
Pfam Domains
Protein Families
SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

761

Amino Acids

87.38

Weight (kDa)

5.77

Isoelectric Point (pI)

44.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQHop_cyclase_N PF13249 102 - 394 9.4e-31 Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C PF13243 416 - 749 4.7e-49 Squalene-hopene cyclase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66960 AT1G66960 AT1G66960 AT1G66960 AT1G78950 AT1G78950 AT1G78955 AT1G78955 AT1G78955 AT1G78955 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78970 AT1G78970 AT1G78970
fragaria_vesca FvH4_5g29480 FvH4_6g36450 FvH4_6g36450 FvH4_6g36500 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g37620
malus_domestica MD03G1089600.v1.1 MD09G1167700.v1.1 MD09G1168200.v1.1 MD09G1168300.v1.1 MD11G1098600.v1.1 MD17G1158300.v1.1 MD17G1158700.v1.1 MD17G1159500.v1.1 MD17G1160500.v1.1 MD17G1182500.v1.1 MD17G1182700.v1.1 MD17G1245800.v1.1 MD17G1246300.v1.1 MD17G1246600.v1.1
prunus_persica Prupe.3G025700_v2.0.a1 Prupe.3G025700_v2.0.a1 Prupe.3G025800_v2.0.a1 Prupe.3G025900_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026400_v2.0.a1 Prupe.3G026500_v2.0.a1 Prupe.3G026700_v2.0.a1 Prupe.3G026800_v2.0.a1
pyrus_communis pycom03g07120 pycom09g08520 pycom09g08590 pycom09g08610 pycom10g10070 pycom11g08310 pycom16g19880 pycom17g15190 pycom17g15300
rosa_chinensis RchiOBHm_Chr2g0136221 RchiOBHm_Chr2g0148451 RchiOBHm_Chr2g0148501 RchiOBHm_Chr2g0148521 RchiOBHm_Chr2g0148661 RchiOBHm_Chr2g0148701 RchiOBHm_Chr2g0148811 RchiOBHm_Chr2g0148941 RchiOBHm_Chr2g0148981 RchiOBHm_Chr2g0150451 RchiOBHm_Chr2g0150521 RchiOBHm_Chr2g0150611 RchiOBHm_Chr4g0420831 RchiOBHm_Chr5g0049791 RchiOBHm_Chr5g0049801
rosa_laevigata RLG00000007735 RLG00000020351 RLG00000020352 RLG00000020357 RLG00000020358 RLG00000020360 RLG00000029093 RLG00000030686
rosa_multiflora Rmu_co8274513.1_g000001 Rmu_sc0000048.1_g000003 Rmu_sc0000048.1_g000033 Rmu_sc0000048.1_g000039 Rmu_sc0001397.1_g000011 Rmu_sc0001998.1_g000039 Rmu_sc0002070.1_g000055 Rmu_sc0002082.1_g000052 Rmu_sc0003181.1_g000010 Rmu_sc0005104.1_g000005 Rmu_sc0006758.1_g000002 Rmu_sc0008411.1_g000029 Rmu_sc0008411.1_g000030 Rmu_sc0017472.1_g000001 Rmu_sc0017472.1_g000003 Rmu_sc0029876.1_g000001 Rmu_ssc0000480.1_g000024 Rmu_ssc0000480.1_g000036
rosa_roxburghii Rroxscaffold_2G00097070 Rroxscaffold_2G00098540 Rroxscaffold_2G00098560 Rroxscaffold_2G00098570 Rroxscaffold_2G00098650 Rroxscaffold_2G00098710 Rroxscaffold_2G00098740 Rroxscaffold_3G00225830 Rroxscaffold_3G00236870 Rroxscaffold_4G00312240
rosa_rugosa Rorug01G0047000 Rorug02G0372300 Rorug02G0407200 Rorug02G0407300 Rorug02G0408400 Rorug02G0421500 Rorug07G0208900
rosa_samantha Rh2AG380200 Rh2AG465700 Rh2AG465800 Rh2AG466800 Rh2AG467100 Rh2AG467600 Rh2AG480900 Rh2AG481400 Rh2AG481700 Rh2CG073500 Rh2CG452200 Rh2CG453200 Rh2CG453600 Rh2CG454100 Rh2CG467100 Rh2CG467800 Rh2CG468000 Rh2DG403100 Rh2DG487800 Rh2DG487900 Rh2DG488800 Rh2DG502000 Rh2DG502200 Rh2DG502300 Rh2DG503100 Rh3AG319300 Rh4AG227700 Rh4BG371800 Rh5AG327400 Rh5DG350600 Rh7AG349800
rosa_wichuraiana Rw0G010080 Rw2G031110 Rw2G037980 Rw2G037990 Rw2G038000 Rw2G038010 Rw2G038080 Rw2G038100 Rw2G039260 Rw2G039290 Rw2G039470 Rw4G019710 Rw5G030930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 601, 1919
Acc36I ACCTGC 1 cut(s) 89
Acc65I GGTACC 1 cut(s) 101
AccB1I GGYRCC 1 cut(s) 101
AccB7I CCANNNNNTGG 1 cut(s) 1583
AccI GTMKAC 3 cut(s) 737, 1929, 2068
AciI CCGC 4 cut(s) 432, 509, 2111, 2245
AclI AACGTT 1 cut(s) 961
AclWI GGATC 4 cut(s) 1142, 1155, 1696, 2078
AcoI YGGCCR 1 cut(s) 1738
AcsI RAATTY 6 cut(s) 231, 722, 867, 1062, 1331, 2023
AfaI GTAC 9 cut(s) 103, 641, 957, 1673, 1679, 1733, 1805, 1855, 2020
AfiI CCNNNNNNNGG 9 cut(s) 98, 385, 581, 603, 1249, 1583, 2155, 2227, 2251
AflII CTTAAG 1 cut(s) 1316
AgsI TTSAA 9 cut(s) 128, 163, 1021, 1046, 1168, 1271, 1401, 1571, 1793
AjnI CCWGG 4 cut(s) 406, 1595, 1704, 1735
AjuI GAANNNNNNNTTGG 2 cut(s) 2114, 2146
AleI CACNNNNGTG 2 cut(s) 613, 1471
AluBI AGCT 6 cut(s) 131, 287, 1163, 1274, 1529, 2009
AluI AGCT 6 cut(s) 131, 287, 1163, 1274, 1529, 2009
Alw21I GWGCWC 5 cut(s) 441, 1261, 1615, 1691, 1977
Alw26I GTCTC 3 cut(s) 853, 1026, 1569
Alw44I GTGCAC 1 cut(s) 1687
AlwI GGATC 4 cut(s) 1142, 1155, 1696, 2078
AlwNI CAGNNNCTG 1 cut(s) 2161
ApaLI GTGCAC 1 cut(s) 1687
ApoI RAATTY 6 cut(s) 231, 722, 867, 1062, 1331, 2023
AseI ATTAAT 2 cut(s) 1359, 2048
Asp718I GGTACC 1 cut(s) 101
AspLEI GCGC 4 cut(s) 602, 912, 1409, 1920
AspS9I GGNCC 8 cut(s) 166, 362, 512, 572, 830, 1140, 1307, 1657
AsuC2I CCSGG 1 cut(s) 2102
AsuHPI GGTGA 7 cut(s) 269, 321, 1363, 1382, 1526, 2090, 2249
AvaII GGWCC 3 cut(s) 166, 1307, 1657
BaeGI GKGCMC 2 cut(s) 610, 1691
BalI TGGCCA 1 cut(s) 1740
BamHI GGATCC 1 cut(s) 1147
BanI GGYRCC 1 cut(s) 101
BanII GRGCYC 1 cut(s) 2233
BauI CACGAG 1 cut(s) 135
BbsI GAAGAC 1 cut(s) 1800
Bbv12I GWGCWC 5 cut(s) 441, 1261, 1615, 1691, 1977
BceAI ACGGC 1 cut(s) 2155
BcgI CGANNNNNNTGC 4 cut(s) 77, 111, 1909, 1943
BciT130I CCWGG 4 cut(s) 408, 1597, 1706, 1737
BclI TGATCA 2 cut(s) 274, 1429
BcnI CCSGG 1 cut(s) 2102
BcoDI GTCTC 3 cut(s) 853, 1026, 1569
BfaI CTAG 4 cut(s) 174, 516, 1152, 1182
BfmI CTRYAG 3 cut(s) 778, 1458, 1779
BfrI CTTAAG 1 cut(s) 1316
BfuAI ACCTGC 1 cut(s) 89
BlpI GCTNAGC 1 cut(s) 435
Bme1390I CCNGG 5 cut(s) 408, 1597, 1706, 1737, 2102
Bme18I GGWCC 3 cut(s) 166, 1307, 1657
BmgT120I GGNCC 8 cut(s) 166, 362, 512, 572, 830, 1140, 1307, 1657
BmiI GGNNCC 7 cut(s) 103, 513, 574, 1149, 1308, 1309, 1602
BmrFI CCNGG 5 cut(s) 408, 1597, 1706, 1737, 2102
BmrI ACTGGG 3 cut(s) 703, 741, 1840
BmsI GCATC 8 cut(s) 85, 543, 659, 1094, 1116, 1183, 1215, 1534
BmuI ACTGGG 3 cut(s) 703, 741, 1840
BpiI GAAGAC 1 cut(s) 1800
BpmI CTGGAG 2 cut(s) 222, 1898
Bpu1102I GCTNAGC 1 cut(s) 435
BpuEI CTTGAG 3 cut(s) 1322, 1673, 1995
BpuMI CCSGG 1 cut(s) 2102
BsaJI CCNNGG 9 cut(s) 64, 105, 334, 384, 628, 946, 1143, 1596, 1735
BsaWI WCCGGW 1 cut(s) 1367
Bsc4I CCNNNNNNNGG 9 cut(s) 98, 385, 581, 603, 1249, 1583, 2155, 2227, 2251
Bse118I RCCGGY 1 cut(s) 1886
Bse1I ACTGG 7 cut(s) 239, 698, 736, 902, 1243, 1835, 2256
BseBI CCWGG 4 cut(s) 408, 1597, 1706, 1737
BseDI CCNNGG 9 cut(s) 64, 105, 334, 384, 628, 946, 1143, 1596, 1735
BseGI GGATG 7 cut(s) 675, 754, 1109, 1219, 1364, 1442, 2140
BseLI CCNNNNNNNGG 9 cut(s) 98, 385, 581, 603, 1249, 1583, 2155, 2227, 2251
BseMII CTCAG 5 cut(s) 426, 1361, 1460, 1521, 2067
BseNI ACTGG 7 cut(s) 239, 698, 736, 902, 1243, 1835, 2256
BseRI GAGGAG 2 cut(s) 122, 125
BseSI GKGCMC 2 cut(s) 610, 1691
BseYI CCCAGC 4 cut(s) 492, 1137, 1283, 1965
BsgI GTGCAG 2 cut(s) 485, 2022
Bsh1285I CGRYCG 1 cut(s) 2068
BshNI GGYRCC 1 cut(s) 101
BsiEI CGRYCG 1 cut(s) 2068
BsiHKAI GWGCWC 5 cut(s) 441, 1261, 1615, 1691, 1977
BsiSI CCGG 3 cut(s) 1368, 1887, 2101
BslFI GGGAC 4 cut(s) 510, 986, 1320, 1483
BslI CCNNNNNNNGG 9 cut(s) 98, 385, 581, 603, 1249, 1583, 2155, 2227, 2251
BsmAI GTCTC 3 cut(s) 853, 1026, 1569
BsmFI GGGAC 4 cut(s) 510, 986, 1320, 1483
BsmI GAATGC 3 cut(s) 290, 322, 2188
Bsp1286I GDGCHC 7 cut(s) 441, 610, 1261, 1615, 1691, 1977, 2233
Bsp1407I TGTACA 1 cut(s) 955
Bsp143I GATC 9 cut(s) 9, 274, 1026, 1147, 1429, 1701, 1755, 2083, 2274
Bsp1720I GCTNAGC 1 cut(s) 435
Bsp19I CCATGG 3 cut(s) 64, 334, 946
BspACI CCGC 4 cut(s) 432, 509, 2111, 2245
BspCNI CTCAG 5 cut(s) 427, 1360, 1461, 1522, 2068
BspLI GGNNCC 7 cut(s) 103, 513, 574, 1149, 1308, 1309, 1602
BspMAI CTGCAG 2 cut(s) 1462, 1783
BspMI ACCTGC 1 cut(s) 89
BspPI GGATC 4 cut(s) 1142, 1155, 1696, 2078
BspT107I GGYRCC 1 cut(s) 101
BspTI CTTAAG 1 cut(s) 1316
BsrFI RCCGGY 1 cut(s) 1886
BsrGI TGTACA 1 cut(s) 955
BsrI ACTGG 7 cut(s) 239, 698, 736, 902, 1243, 1835, 2256
BssAI RCCGGY 1 cut(s) 1886
BssECI CCNNGG 9 cut(s) 64, 105, 334, 384, 628, 946, 1143, 1596, 1735
BssMI GATC 9 cut(s) 9, 274, 1026, 1147, 1429, 1701, 1755, 2083, 2274
BssSI CACGAG 1 cut(s) 135
BssT1I CCWWGG 4 cut(s) 64, 334, 384, 946
Bst2BI CACGAG 1 cut(s) 135
Bst2UI CCWGG 4 cut(s) 408, 1597, 1706, 1737
Bst6I CTCTTC 1 cut(s) 1572
BstAFI CTTAAG 1 cut(s) 1316
BstAUI TGTACA 1 cut(s) 955
BstC8I GCNNGC 9 cut(s) 117, 348, 361, 598, 1013, 1131, 1135, 1281, 1888
BstDEI CTNAG 6 cut(s) 206, 435, 1347, 1469, 1530, 2076
BstDSI CCRYGG 4 cut(s) 64, 334, 628, 946
BstENI CCTNNNNNAGG 1 cut(s) 601
BstF5I GGATG 7 cut(s) 675, 754, 1109, 1219, 1364, 1442, 2140
BstHHI GCGC 4 cut(s) 602, 912, 1409, 1920
BstKTI GATC 9 cut(s) 12, 277, 1029, 1150, 1432, 1704, 1758, 2086, 2277
BstMAI GTCTC 3 cut(s) 853, 1026, 1569
BstMBI GATC 9 cut(s) 9, 274, 1026, 1147, 1429, 1701, 1755, 2083, 2274
BstMCI CGRYCG 1 cut(s) 2068
BstMWI GCNNNNNNNGC 5 cut(s) 287, 356, 907, 1139, 1280
BstNI CCWGG 4 cut(s) 408, 1597, 1706, 1737
BstNSI RCATGY 1 cut(s) 537
BstSCI CCNGG 5 cut(s) 406, 1595, 1704, 1735, 2100
BstSFI CTRYAG 3 cut(s) 778, 1458, 1779
BstSLI GKGCMC 2 cut(s) 610, 1691
BstV2I GAAGAC 1 cut(s) 1800
BstX2I RGATCY 2 cut(s) 1147, 2083
BstXI CCANNNNNNTGG 1 cut(s) 726
BstYI RGATCY 2 cut(s) 1147, 2083
BtgI CCRYGG 4 cut(s) 64, 334, 628, 946
BtsCI GGATG 7 cut(s) 675, 754, 1109, 1219, 1364, 1442, 2140
BtsI GCAGTG 2 cut(s) 1455, 1920
BtsIMutI CAGTG 4 cut(s) 1455, 1466, 1920, 2249
BveI ACCTGC 1 cut(s) 89
Cac8I GCNNGC 9 cut(s) 117, 348, 361, 598, 1013, 1131, 1135, 1281, 1888
CaiI CAGNNNCTG 1 cut(s) 2161
CfoI GCGC 4 cut(s) 602, 912, 1409, 1920
Cfr10I RCCGGY 1 cut(s) 1886
Cfr13I GGNCC 8 cut(s) 166, 362, 512, 572, 830, 1140, 1307, 1657
Csp6I GTAC 9 cut(s) 102, 640, 956, 1672, 1678, 1732, 1804, 1854, 2019
CspCI CAANNNNNGTGG 2 cut(s) 805, 840
CviQI GTAC 9 cut(s) 102, 640, 956, 1672, 1678, 1732, 1804, 1854, 2019
DdeI CTNAG 6 cut(s) 206, 435, 1347, 1469, 1530, 2076
DpnI GATC 9 cut(s) 11, 276, 1028, 1149, 1431, 1703, 1757, 2085, 2276
DpnII GATC 9 cut(s) 9, 274, 1026, 1147, 1429, 1701, 1755, 2083, 2274
DraI TTTAAA 1 cut(s) 223
EaeI YGGCCR 1 cut(s) 1738
Eam1104I CTCTTC 1 cut(s) 1572
EarI CTCTTC 1 cut(s) 1572
Eco130I CCWWGG 4 cut(s) 64, 334, 384, 946
Eco147I AGGCCT 1 cut(s) 1709
Eco24I GRGCYC 1 cut(s) 2233
Eco32I GATATC 2 cut(s) 413, 1344
Eco47I GGWCC 3 cut(s) 166, 1307, 1657
EcoNI CCTNNNNNAGG 1 cut(s) 601
EcoO109I RGGNCCY 3 cut(s) 512, 1307, 1657
EcoRI GAATTC 2 cut(s) 722, 1331
EcoRII CCWGG 4 cut(s) 406, 1595, 1704, 1735
EcoRV GATATC 2 cut(s) 413, 1344
EcoT14I CCWWGG 4 cut(s) 64, 334, 384, 946
EcoT38I GRGCYC 1 cut(s) 2233
ErhI CCWWGG 4 cut(s) 64, 334, 384, 946
FalI AAGNNNNNCTT 4 cut(s) 437, 469, 1263, 1295
FaqI GGGAC 4 cut(s) 510, 986, 1320, 1483
FauI CCCGC 1 cut(s) 502
FauNDI CATATG 1 cut(s) 271
FbaI TGATCA 2 cut(s) 274, 1429
FblI GTMKAC 3 cut(s) 737, 1929, 2068
FokI GGATG 7 cut(s) 682, 741, 1116, 1226, 1351, 1449, 2147
FriOI GRGCYC 1 cut(s) 2233
FspBI CTAG 4 cut(s) 174, 516, 1152, 1182
FspI TGCGCA 2 cut(s) 601, 1919
GlaI GCGC 4 cut(s) 601, 911, 1408, 1919
GsaI CCCAGC 4 cut(s) 496, 1141, 1287, 1969
GsuI CTGGAG 2 cut(s) 222, 1898
HapII CCGG 3 cut(s) 1368, 1887, 2101
HhaI GCGC 4 cut(s) 602, 912, 1409, 1920
Hin6I GCGC 4 cut(s) 600, 910, 1407, 1918
HinP1I GCGC 4 cut(s) 600, 910, 1407, 1918
HincII GTYRAC 4 cut(s) 738, 786, 1930, 2069
HindII GTYRAC 4 cut(s) 738, 786, 1930, 2069
HindIII AAGCTT 2 cut(s) 1161, 1272
HinfI GANTC 9 cut(s) 619, 676, 739, 878, 1218, 1267, 1288, 1836, 2059
HpaII CCGG 3 cut(s) 1368, 1887, 2101
HphI GGTGA 7 cut(s) 269, 321, 1363, 1382, 1526, 2090, 2249
Hpy166II GTNNAC 7 cut(s) 528, 738, 786, 956, 1689, 1930, 2069
Hpy188I TCNGA 9 cut(s) 201, 566, 1031, 1103, 1208, 1423, 1450, 2045, 2058
Hpy8I GTNNAC 7 cut(s) 528, 738, 786, 956, 1689, 1930, 2069
Hpy99I CGWCG 1 cut(s) 995
HpyAV CCTTC 6 cut(s) 63, 319, 439, 692, 1740, 1744
HpyCH4IV ACGT 1 cut(s) 961
HpyF10VI GCNNNNNNNGC 5 cut(s) 287, 356, 907, 1139, 1280
HpyF3I CTNAG 6 cut(s) 206, 435, 1347, 1469, 1530, 2076
HpySE526I ACGT 1 cut(s) 961
HspAI GCGC 4 cut(s) 600, 910, 1407, 1918
KflI GGGWCCC 1 cut(s) 1307
KpnI GGTACC 1 cut(s) 105
KroI GCCGGC 1 cut(s) 1886
KroNI GCCGGC 1 cut(s) 1888
Ksp22I TGATCA 2 cut(s) 274, 1429
Kzo9I GATC 9 cut(s) 9, 274, 1026, 1147, 1429, 1701, 1755, 2083, 2274
LmnI GCTCC 3 cut(s) 112, 1600, 1610
LweI GCATC 8 cut(s) 85, 543, 659, 1094, 1116, 1183, 1215, 1534
MaeI CTAG 4 cut(s) 174, 516, 1152, 1182
MaeII ACGT 1 cut(s) 961
MaeIII GTNAC 6 cut(s) 458, 587, 1088, 1186, 1370, 1391
MalI GATC 9 cut(s) 11, 276, 1028, 1149, 1431, 1703, 1757, 2085, 2276
MboI GATC 9 cut(s) 9, 274, 1026, 1147, 1429, 1701, 1755, 2083, 2274
MfeI CAATTG 2 cut(s) 1053, 2129
MflI RGATCY 2 cut(s) 1147, 2083
MhlI GDGCHC 7 cut(s) 441, 610, 1261, 1615, 1691, 1977, 2233
MlsI TGGCCA 1 cut(s) 1740
MluNI TGGCCA 1 cut(s) 1740
MlyI GAGTC 1 cut(s) 733
MmeI TCCRAC 2 cut(s) 1605, 2111
Mox20I TGGCCA 1 cut(s) 1740
MroNI GCCGGC 1 cut(s) 1886
MscI TGGCCA 1 cut(s) 1740
MseI TTAA 8 cut(s) 222, 894, 1293, 1317, 1359, 1712, 2048, 2117
MslI CAYNNNNRTG 6 cut(s) 477, 613, 918, 951, 1471, 1618
Msp20I TGGCCA 1 cut(s) 1740
MspA1I CMGCKG 2 cut(s) 287, 434
MspCI CTTAAG 1 cut(s) 1316
MspI CCGG 3 cut(s) 1368, 1887, 2101
MspR9I CCNGG 5 cut(s) 408, 1597, 1706, 1737, 2102
MunI CAATTG 2 cut(s) 1053, 2129
Mva1269I GAATGC 3 cut(s) 290, 322, 2188
MvaI CCWGG 4 cut(s) 408, 1597, 1706, 1737
MwoI GCNNNNNNNGC 5 cut(s) 287, 356, 907, 1139, 1280
NaeI GCCGGC 1 cut(s) 1888
NciI CCSGG 1 cut(s) 2102
NcoI CCATGG 3 cut(s) 64, 334, 946
NdeI CATATG 1 cut(s) 271
NdeII GATC 9 cut(s) 9, 274, 1026, 1147, 1429, 1701, 1755, 2083, 2274
NgoMIV GCCGGC 1 cut(s) 1886
NlaIV GGNNCC 7 cut(s) 103, 513, 574, 1149, 1308, 1309, 1602
NmeAIII GCCGAG 1 cut(s) 1168
NmuCI GTSAC 3 cut(s) 587, 1370, 1391
NsbI TGCGCA 2 cut(s) 601, 1919
NspI RCATGY 1 cut(s) 537
OliI CACNNNNGTG 2 cut(s) 613, 1471
PceI AGGCCT 1 cut(s) 1709
PcsI WCGNNNNNNNCGW 2 cut(s) 958, 1680
PctI GAATGC 3 cut(s) 290, 322, 2188
PdiI GCCGGC 1 cut(s) 1888
PfeI GAWTC 8 cut(s) 619, 676, 878, 1218, 1267, 1288, 1836, 2059
PflFI GACNNNGTC 2 cut(s) 733, 1661
PflMI CCANNNNNTGG 1 cut(s) 1583
PfoI TCCNGGA 1 cut(s) 406
PleI GAGTC 1 cut(s) 733
PpsI GAGTC 1 cut(s) 733
PpuMI RGGWCCY 2 cut(s) 1307, 1657
PshBI ATTAAT 2 cut(s) 1359, 2048
Psp1406I AACGTT 1 cut(s) 961
Psp5II RGGWCCY 2 cut(s) 1307, 1657
Psp6I CCWGG 4 cut(s) 406, 1595, 1704, 1735
PspFI CCCAGC 4 cut(s) 492, 1137, 1283, 1965
PspGI CCWGG 4 cut(s) 406, 1595, 1704, 1735
PspN4I GGNNCC 7 cut(s) 103, 513, 574, 1149, 1308, 1309, 1602
PspPI GGNCC 8 cut(s) 166, 362, 512, 572, 830, 1140, 1307, 1657
PspPPI RGGWCCY 2 cut(s) 1307, 1657
PsrI GAACNNNNNNTAC 2 cut(s) 1073, 1105
PstI CTGCAG 2 cut(s) 1462, 1783
PstNI CAGNNNCTG 1 cut(s) 2161
PsuI RGATCY 2 cut(s) 1147, 2083
PsyI GACNNNGTC 2 cut(s) 733, 1661
PvuII CAGCTG 1 cut(s) 287
RsaI GTAC 9 cut(s) 103, 641, 957, 1673, 1679, 1733, 1805, 1855, 2020
RsaNI GTAC 9 cut(s) 102, 640, 956, 1672, 1678, 1732, 1804, 1854, 2019
RseI CAYNNNNRTG 6 cut(s) 477, 613, 918, 951, 1471, 1618
SalI GTCGAC 3 cut(s) 736, 1928, 2067
SaqAI TTAA 8 cut(s) 222, 894, 1293, 1317, 1359, 1712, 2048, 2117
Sau3AI GATC 9 cut(s) 9, 274, 1026, 1147, 1429, 1701, 1755, 2083, 2274
Sau96I GGNCC 8 cut(s) 166, 362, 512, 572, 830, 1140, 1307, 1657
SchI GAGTC 1 cut(s) 733
ScrFI CCNGG 5 cut(s) 408, 1597, 1706, 1737, 2102
SduI GDGCHC 7 cut(s) 441, 610, 1261, 1615, 1691, 1977, 2233
SfaNI GCATC 8 cut(s) 85, 543, 659, 1094, 1116, 1183, 1215, 1534
SfcI CTRYAG 3 cut(s) 778, 1458, 1779
SinI GGWCC 3 cut(s) 166, 1307, 1657
SmiMI CAYNNNNRTG 6 cut(s) 477, 613, 918, 951, 1471, 1618
SmlI CTYRAG 4 cut(s) 1316, 1337, 1652, 2010
SmoI CTYRAG 4 cut(s) 1316, 1337, 1652, 2010
SseBI AGGCCT 1 cut(s) 1709
SsiI CCGC 4 cut(s) 432, 509, 2111, 2245
SspMI CTAG 4 cut(s) 174, 516, 1152, 1182
StuI AGGCCT 1 cut(s) 1709
StyD4I CCNGG 5 cut(s) 406, 1595, 1704, 1735, 2100
StyI CCWWGG 4 cut(s) 64, 334, 384, 946
TaiI ACGT 1 cut(s) 964
TaqI TCGA 7 cut(s) 87, 737, 993, 1683, 1700, 1929, 2068
TatI WGTACW 3 cut(s) 955, 1677, 2018
TfiI GAWTC 8 cut(s) 619, 676, 878, 1218, 1267, 1288, 1836, 2059
Tru1I TTAA 8 cut(s) 222, 894, 1293, 1317, 1359, 1712, 2048, 2117
Tru9I TTAA 8 cut(s) 222, 894, 1293, 1317, 1359, 1712, 2048, 2117
TscAI CASTG 4 cut(s) 1462, 1473, 1920, 2256
TseFI GTSAC 3 cut(s) 587, 1370, 1391
Tsp45I GTSAC 3 cut(s) 587, 1370, 1391
TspDTI ATGAA 7 cut(s) 495, 743, 1055, 1086, 1683, 1756, 2135
TspGWI ACGGA 1 cut(s) 1379
TspRI CASTG 4 cut(s) 1462, 1473, 1920, 2256
Tth111I GACNNNGTC 2 cut(s) 733, 1661
Van91I CCANNNNNTGG 1 cut(s) 1583
Vha464I CTTAAG 1 cut(s) 1316
VneI GTGCAC 1 cut(s) 1687
VpaK11BI GGWCC 3 cut(s) 166, 1307, 1657
VspI ATTAAT 2 cut(s) 1359, 2048
XagI CCTNNNNNAGG 1 cut(s) 601
XapI RAATTY 6 cut(s) 231, 722, 867, 1062, 1331, 2023
XceI RCATGY 1 cut(s) 537
XmiI GTMKAC 3 cut(s) 737, 1929, 2068
XspI CTAG 4 cut(s) 174, 516, 1152, 1182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.