Belongs to the terpene cyclase mutase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 2135 |
| Acc16I | TGCGCA | 1 cut(s) | 1931 |
| Acc65I | GGTACC | 1 cut(s) | 1865 |
| AccB1I | GGYRCC | 1 cut(s) | 1865 |
| AciI | CCGC | 5 cut(s) | 137, 163, 356, 1402, 2084 |
| AclWI | GGATC | 7 cut(s) | 89, 978, 1154, 1192, 1205, 1426, 2031 |
| AcoI | YGGCCR | 2 cut(s) | 343, 592 |
| AcsI | RAATTY | 6 cut(s) | 456, 564, 731, 876, 1658, 1815 |
| AcuI | CTGAAG | 3 cut(s) | 135, 1176, 1242 |
| AfaI | GTAC | 6 cut(s) | 918, 1400, 1553, 1794, 1867, 2049 |
| AfeI | AGCGCT | 1 cut(s) | 1547 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 104, 169, 587, 665, 2027, 2245, 2288 |
| AflII | CTTAAG | 1 cut(s) | 11 |
| AflIII | ACRYGT | 2 cut(s) | 508, 1690 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 509 |
| AhlI | ACTAGT | 1 cut(s) | 1268 |
| AjnI | CCWGG | 5 cut(s) | 1200, 1607, 1716, 1747, 1868 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 209, 241 |
| Alw21I | GWGCWC | 1 cut(s) | 1703 |
| Alw26I | GTCTC | 2 cut(s) | 1164, 2078 |
| Alw44I | GTGCAC | 1 cut(s) | 1699 |
| AlwI | GGATC | 7 cut(s) | 89, 978, 1154, 1192, 1205, 1426, 2031 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 110, 1898 |
| Aor51HI | AGCGCT | 1 cut(s) | 1547 |
| AoxI | GGCC | 6 cut(s) | 343, 350, 367, 592, 838, 1022 |
| ApaLI | GTGCAC | 1 cut(s) | 1699 |
| ApeKI | GCWGC | 6 cut(s) | 1625, 1892, 1895, 1922, 2123, 2219 |
| ApoI | RAATTY | 6 cut(s) | 456, 564, 731, 876, 1658, 1815 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 664, 696 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 65, 224 |
| Asp718I | GGTACC | 1 cut(s) | 1865 |
| AspA2I | CCTAGG | 1 cut(s) | 1111 |
| AspLEI | GCGC | 2 cut(s) | 1548, 1932 |
| AspS9I | GGNCC | 4 cut(s) | 368, 578, 839, 1023 |
| AsuHPI | GGTGA | 5 cut(s) | 197, 1394, 1538, 1774, 2171 |
| AvaII | GGWCC | 1 cut(s) | 578 |
| AvrII | CCTAGG | 1 cut(s) | 1111 |
| AxyI | CCTNAGG | 1 cut(s) | 212 |
| BaeGI | GKGCMC | 2 cut(s) | 1703, 1755 |
| BalI | TGGCCA | 2 cut(s) | 345, 594 |
| BamHI | GGATCC | 1 cut(s) | 1197 |
| BanI | GGYRCC | 1 cut(s) | 1865 |
| BanII | GRGCYC | 1 cut(s) | 2251 |
| BbsI | GAAGAC | 2 cut(s) | 137, 2182 |
| Bbv12I | GWGCWC | 1 cut(s) | 1703 |
| BbvCI | CCTCAGC | 2 cut(s) | 126, 2169 |
| BbvI | GCAGC | 6 cut(s) | 1637, 1882, 1904, 1934, 2135, 2231 |
| BciT130I | CCWGG | 5 cut(s) | 1202, 1609, 1718, 1749, 1870 |
| BciVI | GTATCC | 2 cut(s) | 957, 1258 |
| BclI | TGATCA | 2 cut(s) | 634, 1038 |
| BcoDI | GTCTC | 2 cut(s) | 1164, 2078 |
| BcuI | ACTAGT | 1 cut(s) | 1268 |
| BfaI | CTAG | 5 cut(s) | 1112, 1194, 1269, 1332, 1880 |
| BfmI | CTRYAG | 4 cut(s) | 784, 1470, 1580, 1920 |
| BfoI | RGCGCY | 1 cut(s) | 1549 |
| BfrI | CTTAAG | 1 cut(s) | 11 |
| BfuI | GTATCC | 2 cut(s) | 957, 1258 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1151 |
| BisI | GCNGC | 7 cut(s) | 138, 1626, 1893, 1896, 1923, 2124, 2220 |
| BlnI | CCTAGG | 1 cut(s) | 1111 |
| BlsI | GCNGC | 7 cut(s) | 139, 1627, 1894, 1897, 1924, 2125, 2221 |
| Bme1390I | CCNGG | 5 cut(s) | 1202, 1609, 1718, 1749, 1870 |
| Bme18I | GGWCC | 1 cut(s) | 578 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 509 |
| BmgT120I | GGNCC | 4 cut(s) | 368, 578, 839, 1023 |
| BmiI | GGNNCC | 4 cut(s) | 1199, 1539, 1867, 2274 |
| BmrFI | CCNGG | 5 cut(s) | 1202, 1609, 1718, 1749, 1870 |
| BmrI | ACTGGG | 1 cut(s) | 174 |
| BmsI | GCATC | 6 cut(s) | 283, 427, 772, 1195, 1227, 1497 |
| BmuI | ACTGGG | 1 cut(s) | 174 |
| BpiI | GAAGAC | 2 cut(s) | 137, 2182 |
| BpmI | CTGGAG | 2 cut(s) | 228, 2205 |
| Bpu10I | CCTNAGC | 3 cut(s) | 126, 1542, 2169 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1440 |
| BsaI | GGTCTC | 1 cut(s) | 2078 |
| BsaJI | CCNNGG | 5 cut(s) | 39, 959, 1111, 1748, 2286 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 209, 226, 239, 256, 957, 987 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 104, 169, 587, 665, 2027, 2245, 2288 |
| Bse1I | ACTGG | 5 cut(s) | 180, 245, 1296, 2154, 2188 |
| Bse21I | CCTNAGG | 1 cut(s) | 212 |
| Bse3DI | GCAATG | 2 cut(s) | 445, 1939 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1440 |
| BseBI | CCWGG | 5 cut(s) | 1202, 1609, 1718, 1749, 1870 |
| BseDI | CCNNGG | 5 cut(s) | 39, 959, 1111, 1748, 2286 |
| BseGI | GGATG | 8 cut(s) | 763, 798, 955, 1236, 1315, 1454, 1744, 1828 |
| BseJI | GATNNNNATC | 1 cut(s) | 1440 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 104, 169, 587, 665, 2027, 2245, 2288 |
| BseMI | GCAATG | 2 cut(s) | 445, 1939 |
| BseMII | CTCAG | 6 cut(s) | 117, 336, 559, 1373, 1533, 2183 |
| BseNI | ACTGG | 5 cut(s) | 180, 245, 1296, 2154, 2188 |
| BseSI | GKGCMC | 2 cut(s) | 1703, 1755 |
| BseXI | GCAGC | 6 cut(s) | 1637, 1882, 1904, 1934, 2135, 2231 |
| BsgI | GTGCAG | 1 cut(s) | 2142 |
| BshFI | GGCC | 6 cut(s) | 345, 352, 369, 594, 840, 1024 |
| BshNI | GGYRCC | 1 cut(s) | 1865 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1703 |
| BslFI | GGGAC | 2 cut(s) | 516, 2100 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 104, 169, 587, 665, 2027, 2245, 2288 |
| BsmAI | GTCTC | 2 cut(s) | 1164, 2078 |
| BsmFI | GGGAC | 2 cut(s) | 516, 2100 |
| BsmI | GAATGC | 1 cut(s) | 207 |
| BsnI | GGCC | 6 cut(s) | 345, 352, 369, 594, 840, 1024 |
| Bso31I | GGTCTC | 1 cut(s) | 2078 |
| Bsp1286I | GDGCHC | 3 cut(s) | 1703, 1755, 2251 |
| Bsp1407I | TGTACA | 1 cut(s) | 1792 |
| BspACI | CCGC | 5 cut(s) | 137, 163, 356, 1402, 2084 |
| BspANI | GGCC | 6 cut(s) | 345, 352, 369, 594, 840, 1024 |
| BspCNI | CTCAG | 6 cut(s) | 118, 335, 558, 1372, 1534, 2182 |
| BspLI | GGNNCC | 4 cut(s) | 1199, 1539, 1867, 2274 |
| BspMAI | CTGCAG | 2 cut(s) | 1474, 1924 |
| BspPI | GGATC | 7 cut(s) | 89, 978, 1154, 1192, 1205, 1426, 2031 |
| BspQI | GCTCTTC | 2 cut(s) | 111, 1059 |
| BspT107I | GGYRCC | 1 cut(s) | 1865 |
| BspTI | CTTAAG | 1 cut(s) | 11 |
| BspTNI | GGTCTC | 1 cut(s) | 2078 |
| BsrDI | GCAATG | 2 cut(s) | 445, 1939 |
| BsrGI | TGTACA | 1 cut(s) | 1792 |
| BsrI | ACTGG | 5 cut(s) | 180, 245, 1296, 2154, 2188 |
| BssECI | CCNNGG | 5 cut(s) | 39, 959, 1111, 1748, 2286 |
| BssT1I | CCWWGG | 3 cut(s) | 959, 1111, 2286 |
| Bst2UI | CCWGG | 5 cut(s) | 1202, 1609, 1718, 1749, 1870 |
| Bst4CI | ACNGT | 3 cut(s) | 428, 1644, 2244 |
| Bst6I | CTCTTC | 4 cut(s) | 111, 1014, 1059, 2118 |
| BstAFI | CTTAAG | 1 cut(s) | 11 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 110 |
| BstAUI | TGTACA | 1 cut(s) | 1792 |
| BstDEI | CTNAG | 8 cut(s) | 126, 212, 322, 438, 545, 1359, 1542, 2169 |
| BstEII | GGTNACC | 1 cut(s) | 185 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 2025 |
| BstF5I | GGATG | 8 cut(s) | 763, 798, 955, 1236, 1315, 1454, 1744, 1828 |
| BstH2I | RGCGCY | 1 cut(s) | 1549 |
| BstHHI | GCGC | 2 cut(s) | 1548, 1932 |
| BstMAI | GTCTC | 2 cut(s) | 1164, 2078 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 110, 332, 362, 1151, 1283, 1292, 1401, 1625, 2090 |
| BstNI | CCWGG | 5 cut(s) | 1202, 1609, 1718, 1749, 1870 |
| BstNSI | RCATGY | 6 cut(s) | 512, 805, 1141, 1399, 1694, 2209 |
| BstPI | GGTNACC | 1 cut(s) | 185 |
| BstSCI | CCNGG | 5 cut(s) | 1200, 1607, 1716, 1747, 1868 |
| BstSFI | CTRYAG | 4 cut(s) | 784, 1470, 1580, 1920 |
| BstSLI | GKGCMC | 2 cut(s) | 1703, 1755 |
| BstV1I | GCAGC | 6 cut(s) | 1637, 1882, 1904, 1934, 2135, 2231 |
| BstV2I | GAAGAC | 2 cut(s) | 137, 2182 |
| BstX2I | RGATCY | 3 cut(s) | 970, 1159, 1197 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 735 |
| BstYI | RGATCY | 3 cut(s) | 970, 1159, 1197 |
| Bsu36I | CCTNAGG | 1 cut(s) | 212 |
| BsuI | GTATCC | 2 cut(s) | 957, 1258 |
| BsuRI | GGCC | 6 cut(s) | 345, 352, 369, 594, 840, 1024 |
| BtgZI | GCGATG | 1 cut(s) | 354 |
| BtsCI | GGATG | 8 cut(s) | 763, 798, 955, 1236, 1315, 1454, 1744, 1828 |
| BtsI | GCAGTG | 1 cut(s) | 1467 |
| BtsIMutI | CAGTG | 5 cut(s) | 187, 433, 1467, 2181, 2240 |
| CaiI | CAGNNNCTG | 2 cut(s) | 110, 1898 |
| CfoI | GCGC | 2 cut(s) | 1548, 1932 |
| Cfr13I | GGNCC | 4 cut(s) | 368, 578, 839, 1023 |
| CseI | GACGC | 2 cut(s) | 110, 1966 |
| CsiI | ACCWGGT | 1 cut(s) | 1868 |
| Csp6I | GTAC | 6 cut(s) | 917, 1399, 1552, 1793, 1866, 2048 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1576, 1611 |
| CviQI | GTAC | 6 cut(s) | 917, 1399, 1552, 1793, 1866, 2048 |
| DdeI | CTNAG | 8 cut(s) | 126, 212, 322, 438, 545, 1359, 1542, 2169 |
| DraI | TTTAAA | 1 cut(s) | 1482 |
| DriI | GACNNNNNGTC | 1 cut(s) | 509 |
| EaeI | YGGCCR | 2 cut(s) | 343, 592 |
| Eam1104I | CTCTTC | 4 cut(s) | 111, 1014, 1059, 2118 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 509 |
| EarI | CTCTTC | 4 cut(s) | 111, 1014, 1059, 2118 |
| Eco130I | CCWWGG | 3 cut(s) | 959, 1111, 2286 |
| Eco24I | GRGCYC | 1 cut(s) | 2251 |
| Eco31I | GGTCTC | 1 cut(s) | 2078 |
| Eco47I | GGWCC | 1 cut(s) | 578 |
| Eco47III | AGCGCT | 1 cut(s) | 1547 |
| Eco57I | CTGAAG | 3 cut(s) | 135, 1176, 1242 |
| Eco81I | CCTNAGG | 1 cut(s) | 212 |
| Eco91I | GGTNACC | 1 cut(s) | 185 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 2025 |
| EcoO109I | RGGNCCY | 2 cut(s) | 368, 578 |
| EcoO65I | GGTNACC | 1 cut(s) | 185 |
| EcoRI | GAATTC | 2 cut(s) | 564, 1658 |
| EcoRII | CCWGG | 5 cut(s) | 1200, 1607, 1716, 1747, 1868 |
| EcoT14I | CCWWGG | 3 cut(s) | 959, 1111, 2286 |
| EcoT22I | ATGCAT | 2 cut(s) | 298, 1139 |
| EcoT38I | GRGCYC | 1 cut(s) | 2251 |
| ErhI | CCWWGG | 3 cut(s) | 959, 1111, 2286 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1275, 1307 |
| FaqI | GGGAC | 2 cut(s) | 516, 2100 |
| FauI | CCCGC | 1 cut(s) | 2077 |
| FauNDI | CATATG | 1 cut(s) | 1861 |
| FbaI | TGATCA | 2 cut(s) | 634, 1038 |
| Fnu4HI | GCNGC | 7 cut(s) | 138, 1626, 1893, 1896, 1923, 2124, 2220 |
| FokI | GGATG | 8 cut(s) | 750, 805, 942, 1243, 1322, 1461, 1731, 1835 |
| FriOI | GRGCYC | 1 cut(s) | 2251 |
| Fsp4HI | GCNGC | 7 cut(s) | 138, 1626, 1893, 1896, 1923, 2124, 2220 |
| FspBI | CTAG | 5 cut(s) | 1112, 1194, 1269, 1332, 1880 |
| FspI | TGCGCA | 1 cut(s) | 1931 |
| GlaI | GCGC | 2 cut(s) | 1547, 1931 |
| GluI | GCNGC | 7 cut(s) | 138, 1626, 1893, 1896, 1923, 2124, 2220 |
| GsuI | CTGGAG | 2 cut(s) | 228, 2205 |
| HaeII | RGCGCY | 1 cut(s) | 1549 |
| HaeIII | GGCC | 6 cut(s) | 345, 352, 369, 594, 840, 1024 |
| HgaI | GACGC | 2 cut(s) | 110, 1966 |
| HhaI | GCGC | 2 cut(s) | 1548, 1932 |
| Hin6I | GCGC | 2 cut(s) | 1546, 1930 |
| HinP1I | GCGC | 2 cut(s) | 1546, 1930 |
| HindIII | AAGCTT | 2 cut(s) | 8, 1284 |
| HinfI | GANTC | 4 cut(s) | 35, 628, 1556, 2187 |
| HphI | GGTGA | 5 cut(s) | 197, 1394, 1538, 1774, 2171 |
| Hpy166II | GTNNAC | 5 cut(s) | 313, 1425, 1701, 1795, 2003 |
| Hpy188I | TCNGA | 8 cut(s) | 483, 572, 1043, 1435, 1462, 1516, 1653, 1822 |
| Hpy188III | TCNNGA | 8 cut(s) | 98, 632, 708, 1180, 1256, 1439, 2024, 2194 |
| Hpy8I | GTNNAC | 5 cut(s) | 313, 1425, 1701, 1795, 2003 |
| Hpy99I | CGWCG | 1 cut(s) | 104 |
| HpyCH4III | ACNGT | 3 cut(s) | 428, 1644, 2244 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 110, 332, 362, 1151, 1283, 1292, 1401, 1625, 2090 |
| HpyF3I | CTNAG | 8 cut(s) | 126, 212, 322, 438, 545, 1359, 1542, 2169 |
| HspAI | GCGC | 2 cut(s) | 1546, 1930 |
| KpnI | GGTACC | 1 cut(s) | 1869 |
| Ksp22I | TGATCA | 2 cut(s) | 634, 1038 |
| LguI | GCTCTTC | 2 cut(s) | 111, 1059 |
| LmnI | GCTCC | 2 cut(s) | 442, 1537 |
| Lsp1109I | GCAGC | 6 cut(s) | 1637, 1882, 1904, 1934, 2135, 2231 |
| LweI | GCATC | 6 cut(s) | 283, 427, 772, 1195, 1227, 1497 |
| MabI | ACCWGGT | 1 cut(s) | 1868 |
| MaeI | CTAG | 5 cut(s) | 1112, 1194, 1269, 1332, 1880 |
| MaeIII | GTNAC | 5 cut(s) | 185, 464, 646, 1060, 1382 |
| MfeI | CAATTG | 2 cut(s) | 863, 904 |
| MflI | RGATCY | 3 cut(s) | 970, 1159, 1197 |
| MhlI | GDGCHC | 3 cut(s) | 1703, 1755, 2251 |
| MlsI | TGGCCA | 2 cut(s) | 345, 594 |
| MluNI | TGGCCA | 2 cut(s) | 345, 594 |
| MlyI | GAGTC | 1 cut(s) | 2196 |
| MmeI | TCCRAC | 1 cut(s) | 1676 |
| Mox20I | TGGCCA | 2 cut(s) | 345, 594 |
| Mph1103I | ATGCAT | 2 cut(s) | 298, 1139 |
| MroXI | GAANNNNTTC | 2 cut(s) | 65, 224 |
| MscI | TGGCCA | 2 cut(s) | 345, 594 |
| MseI | TTAA | 7 cut(s) | 12, 174, 423, 1365, 1481, 1646, 1734 |
| MslI | CAYNNNNRTG | 7 cut(s) | 401, 483, 557, 642, 806, 927, 1689 |
| Msp20I | TGGCCA | 2 cut(s) | 345, 594 |
| MspA1I | CMGCKG | 1 cut(s) | 1895 |
| MspCI | CTTAAG | 1 cut(s) | 11 |
| MspR9I | CCNGG | 5 cut(s) | 1202, 1609, 1718, 1749, 1870 |
| MunI | CAATTG | 2 cut(s) | 863, 904 |
| Mva1269I | GAATGC | 1 cut(s) | 207 |
| MvaI | CCWGG | 5 cut(s) | 1202, 1609, 1718, 1749, 1870 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 110, 332, 362, 1151, 1283, 1292, 1401, 1625, 2090 |
| NdeI | CATATG | 1 cut(s) | 1861 |
| NlaIV | GGNNCC | 4 cut(s) | 1199, 1539, 1867, 2274 |
| NmeAIII | GCCGAG | 1 cut(s) | 20 |
| NmuCI | GTSAC | 4 cut(s) | 185, 646, 1060, 1382 |
| NsbI | TGCGCA | 1 cut(s) | 1931 |
| NsiI | ATGCAT | 2 cut(s) | 298, 1139 |
| NspI | RCATGY | 6 cut(s) | 512, 805, 1141, 1399, 1694, 2209 |
| PaeI | GCATGC | 2 cut(s) | 1141, 2209 |
| PciI | ACATGT | 2 cut(s) | 508, 1690 |
| PciSI | GCTCTTC | 2 cut(s) | 111, 1059 |
| PctI | GAATGC | 1 cut(s) | 207 |
| PdmI | GAANNNNTTC | 2 cut(s) | 65, 224 |
| PfeI | GAWTC | 3 cut(s) | 35, 628, 1556 |
| PfoI | TCCNGGA | 1 cut(s) | 1200 |
| PkrI | GCNGC | 7 cut(s) | 139, 1627, 1894, 1897, 1924, 2125, 2221 |
| PleI | GAGTC | 1 cut(s) | 2195 |
| PpsI | GAGTC | 1 cut(s) | 2195 |
| PpuMI | RGGWCCY | 1 cut(s) | 578 |
| PscI | ACATGT | 2 cut(s) | 508, 1690 |
| PsiI | TTATAA | 1 cut(s) | 2135 |
| Psp5II | RGGWCCY | 1 cut(s) | 578 |
| Psp6I | CCWGG | 5 cut(s) | 1200, 1607, 1716, 1747, 1868 |
| PspEI | GGTNACC | 1 cut(s) | 185 |
| PspGI | CCWGG | 5 cut(s) | 1200, 1607, 1716, 1747, 1868 |
| PspN4I | GGNNCC | 4 cut(s) | 1199, 1539, 1867, 2274 |
| PspPI | GGNCC | 4 cut(s) | 368, 578, 839, 1023 |
| PspPPI | RGGWCCY | 1 cut(s) | 578 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1085, 1117 |
| PstI | CTGCAG | 2 cut(s) | 1474, 1924 |
| PstNI | CAGNNNCTG | 2 cut(s) | 110, 1898 |
| PsuI | RGATCY | 3 cut(s) | 970, 1159, 1197 |
| PvuII | CAGCTG | 1 cut(s) | 1895 |
| RsaI | GTAC | 6 cut(s) | 918, 1400, 1553, 1794, 1867, 2049 |
| RsaNI | GTAC | 6 cut(s) | 917, 1399, 1552, 1793, 1866, 2048 |
| RseI | CAYNNNNRTG | 7 cut(s) | 401, 483, 557, 642, 806, 927, 1689 |
| SapI | GCTCTTC | 2 cut(s) | 111, 1059 |
| SaqAI | TTAA | 7 cut(s) | 12, 174, 423, 1365, 1481, 1646, 1734 |
| SatI | GCNGC | 7 cut(s) | 138, 1626, 1893, 1896, 1923, 2124, 2220 |
| Sau96I | GGNCC | 4 cut(s) | 368, 578, 839, 1023 |
| SchI | GAGTC | 1 cut(s) | 2196 |
| ScrFI | CCNGG | 5 cut(s) | 1202, 1609, 1718, 1749, 1870 |
| SduI | GDGCHC | 3 cut(s) | 1703, 1755, 2251 |
| SexAI | ACCWGGT | 1 cut(s) | 1868 |
| SfaNI | GCATC | 6 cut(s) | 283, 427, 772, 1195, 1227, 1497 |
| SfcI | CTRYAG | 4 cut(s) | 784, 1470, 1580, 1920 |
| SinI | GGWCC | 1 cut(s) | 578 |
| SmiMI | CAYNNNNRTG | 7 cut(s) | 401, 483, 557, 642, 806, 927, 1689 |
| SmlI | CTYRAG | 1 cut(s) | 11 |
| SmoI | CTYRAG | 1 cut(s) | 11 |
| SpeI | ACTAGT | 1 cut(s) | 1268 |
| SphI | GCATGC | 2 cut(s) | 1141, 2209 |
| SsiI | CCGC | 5 cut(s) | 137, 163, 356, 1402, 2084 |
| SspI | AATATT | 1 cut(s) | 1775 |
| SspMI | CTAG | 5 cut(s) | 1112, 1194, 1269, 1332, 1880 |
| StyD4I | CCNGG | 5 cut(s) | 1200, 1607, 1716, 1747, 1868 |
| StyI | CCWWGG | 3 cut(s) | 959, 1111, 2286 |
| TaaI | ACNGT | 3 cut(s) | 428, 1644, 2244 |
| TaqI | TCGA | 4 cut(s) | 513, 683, 1502, 1665 |
| TatI | WGTACW | 1 cut(s) | 1792 |
| TauI | GCSGC | 1 cut(s) | 140 |
| TfiI | GAWTC | 3 cut(s) | 35, 628, 1556 |
| Tru1I | TTAA | 7 cut(s) | 12, 174, 423, 1365, 1481, 1646, 1734 |
| Tru9I | TTAA | 7 cut(s) | 12, 174, 423, 1365, 1481, 1646, 1734 |
| TscAI | CASTG | 5 cut(s) | 187, 433, 1474, 2188, 2247 |
| TseFI | GTSAC | 4 cut(s) | 185, 646, 1060, 1382 |
| TseI | GCWGC | 6 cut(s) | 1625, 1892, 1895, 1922, 2123, 2219 |
| Tsp45I | GTSAC | 4 cut(s) | 185, 646, 1060, 1382 |
| TspGWI | ACGGA | 1 cut(s) | 2265 |
| TspRI | CASTG | 5 cut(s) | 187, 433, 1474, 2188, 2247 |
| Vha464I | CTTAAG | 1 cut(s) | 11 |
| VneI | GTGCAC | 1 cut(s) | 1699 |
| VpaK11BI | GGWCC | 1 cut(s) | 578 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 2025 |
| XapI | RAATTY | 6 cut(s) | 456, 564, 731, 876, 1658, 1815 |
| XceI | RCATGY | 6 cut(s) | 512, 805, 1141, 1399, 1694, 2209 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 774, 1303 |
| XmaJI | CCTAGG | 1 cut(s) | 1111 |
| XmnI | GAANNNNTTC | 2 cut(s) | 65, 224 |
| XspI | CTAG | 5 cut(s) | 1112, 1194, 1269, 1332, 1880 |
| Zsp2I | ATGCAT | 2 cut(s) | 298, 1139 |