MD17G1158300.v1.1

Belongs to the terpene cyclase mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000173
Physical Location & Seq
Forward (+)
15320296 .. 15327073
6778 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1158300.v1.1.491

Sequence Viewer

Length: 2283 bp
ATGTGGAAGCTTAAGGTCGCAGATGGTGGCAATGATCCATACATCTACAGCACAAACAACTTCGTGGGTAGGCAGATATTTAAGTTTGACCCTGAAGCAGGAACTACCGAAGAGCGAGCTGAGGTGGAAGAAGCTCGTCTTCATTTCTACAATACTCGCTATCAGGTTAAGCCCAATAGTGACCTCCTATGGCGAATGCAGTTCCTAAGAGAGAAGAACTTCAAACAGACAATTCCCCCAGTGAAAGTTGAGGATGGAGAGGAAATCACATACGAAAAGGCCACCGCTGCTTTGAGAAGGTCTGTCCACTTCTTTTCAGCTTTGCAGGCTAGTGATGGCCATTGGCCAGCTGAAAATGCCGGCCCGTTGTTTTTCCTTCCTCCTTTGGTCATGTGCACTTACATTACAGGACATCTTAATACTGTATTCCCTACAGAACACCGCAAAGAAATTTTGCGTTACATATACTATCATCAGAACCAAGATGGTGGTTGGGGATTACACATTGAAGGACACAGCACCATGTTCTGCACAGCTCTCAACTACATTTGTATGCGTATTCTTGGAGAAGGCCCTGATGGTGGCCAAGACAAGGCTTGTGCAAGAGCTAGAAAGTGGATTCTTGATCATGGCAGTGTTACCCACATCCCCTCTTGGGGTAAAACTTGGCTTTCGATACTTGGTGTGTTTGAGTGGTCCGGAAGCAACCCAATGCCCCCAGAGTTTTGGATTTTGCCTTCATTTCTTCCTATGCATCCAGCAAAAATGTTTTGCTACAGTAGGATGGTTTACATGCCTATGTCATATTTATATGGGAAAAGATTTGTTGGTCCAATCACACCTCTCATACTACAATTAAGAGAAGAACTCTATGCTCAACCTTATGGCGAAATTAATTGGAAGGGAGTGCGTCGTCACTGCGCTAAAGTGGACATCTACTACCCTCATCCTTGGATACAGGACTTTTTATGGGATAGTCTGTACGTATGTACGGAGCCTCTTCTTACTCGCTGGCCGTTTAACAAGCTGATCAGGAAAAGGGCTCTTGAAGTAACAATGGAGCATGTTCATTATGAAGATGAAAACAGCAGATACATTACCATTGGATGTGTGGAAAAGGTGTTATGCATGCTGGCTTGTTGGGTGGAAGATCCAAATGGGGATTATTTTAAAAAGCATCTTGCCAGGATACCGGATTATTTATGGGTTGCTGAGGATGGGATGAAGATGCAAAGTTTTGGCAGTCAACAGTGGGATACTGGTTTTGCCATTCAAGCTTTGCTTGCTTCCAATCTAACCGATGAAATTGCACCCACACTCGCTAGAGGACATGACTTCGTCAAGAAATCTCAGGTCAAGGACAATCCGTCGGGTGACTTCAAAAGCATGTACCGCCACATTTCCAAAGGATCGTGGACTTTCTCTGATCAAGACCATGGATGGCAAGTTTCTGATTGCACTGCAGAGGGTTTAAAGTGTTGCCTGCTTTTATCGATGATGCCACCAGAGATGGTTGGTGAAAAAATGGAGCCTGAGCGGTTATTCGATGCTGTCAATGTCCTAATTTCCCTACAGAGTAAGAATGGTGGCTTAGCAGCCTGGGAACCAGCAGGAGCAGCAGAATGGTTAGAAATGTTCAATCCCTCAGAATTCTTCGCGGACATTGTGGTTGAGCATGAATACGTTGAGTGCACTTCATCTGCAATCCAGGCTTTAGTTTTGTTTAAGAAGTTATGCCCTGGCCACAGGAAGGAAGAGATTGATCAATTTATTACCGCTGCTGCACTGTACCTTGAAAATGTACAAATGGCAGATGGTTCATGGTACGGGAATTGGGGAGTTTGCTTCACATATGGTACCTGGTTTGCTCTTGGAGGGCTAACTGCTGCTGGTAAGACCTTCAACAATTGTGCAGCCATGCGCAAAGCTATTAGTTTTCTACTCGCAATCCAGAAAGAGAATGGTGGTTGGGGAGAGAGCTACCTTTCATGTCCAAAAAAGGAGTACGTTCCTCTTGAAGGAAACCGATCAAATTTAGTTCACACAGCTTGGGCTATGATGGGTTTGATTCATGCAGGGCAGGCGGAAAGAGACCCCACACCTCTCCATCGTGCAGCAAAGTTGATAATCAATTCTCAGATGGAAAATGGTGATTTTCCTCAGCAGGAAATCACTGGAGCGTTCATGAAGAACTGCATGCTACATTATGCAAATTATAGAAATATTTATCCCCTGTGGGCTCTTGCAGAATACCGAAGGCGGGTTCCATTTGTTGATGTCTAA

Protein Analysis

761

Amino Acids

87.17

Weight (kDa)

6.16

Isoelectric Point (pI)

45.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQHop_cyclase_N PF13249 99 - 360 2.6e-35 Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C PF13243 417 - 753 3.9e-50 Squalene-hopene cyclase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66960 AT1G66960 AT1G66960 AT1G66960 AT1G78950 AT1G78950 AT1G78955 AT1G78955 AT1G78955 AT1G78955 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78970 AT1G78970 AT1G78970
fragaria_vesca FvH4_5g29480 FvH4_6g36450 FvH4_6g36450 FvH4_6g36500 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g37620
malus_domestica MD03G1089600.v1.1 MD09G1167700.v1.1 MD09G1168200.v1.1 MD09G1168300.v1.1 MD11G1098600.v1.1 MD17G1158300.v1.1 MD17G1158700.v1.1 MD17G1159500.v1.1 MD17G1160500.v1.1 MD17G1182500.v1.1 MD17G1182700.v1.1 MD17G1245800.v1.1 MD17G1246300.v1.1 MD17G1246600.v1.1
prunus_persica Prupe.3G025700_v2.0.a1 Prupe.3G025700_v2.0.a1 Prupe.3G025800_v2.0.a1 Prupe.3G025900_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026400_v2.0.a1 Prupe.3G026500_v2.0.a1 Prupe.3G026700_v2.0.a1 Prupe.3G026800_v2.0.a1
pyrus_communis pycom03g07120 pycom09g08520 pycom09g08590 pycom09g08610 pycom10g10070 pycom11g08310 pycom16g19880 pycom17g15190 pycom17g15300
rosa_chinensis RchiOBHm_Chr2g0136221 RchiOBHm_Chr2g0148451 RchiOBHm_Chr2g0148501 RchiOBHm_Chr2g0148521 RchiOBHm_Chr2g0148661 RchiOBHm_Chr2g0148701 RchiOBHm_Chr2g0148811 RchiOBHm_Chr2g0148941 RchiOBHm_Chr2g0148981 RchiOBHm_Chr2g0150451 RchiOBHm_Chr2g0150521 RchiOBHm_Chr2g0150611 RchiOBHm_Chr4g0420831 RchiOBHm_Chr5g0049791 RchiOBHm_Chr5g0049801
rosa_laevigata RLG00000007735 RLG00000020351 RLG00000020352 RLG00000020357 RLG00000020358 RLG00000020360 RLG00000029093 RLG00000030686
rosa_multiflora Rmu_co8274513.1_g000001 Rmu_sc0000048.1_g000003 Rmu_sc0000048.1_g000033 Rmu_sc0000048.1_g000039 Rmu_sc0001397.1_g000011 Rmu_sc0001998.1_g000039 Rmu_sc0002070.1_g000055 Rmu_sc0002082.1_g000052 Rmu_sc0003181.1_g000010 Rmu_sc0005104.1_g000005 Rmu_sc0006758.1_g000002 Rmu_sc0008411.1_g000029 Rmu_sc0008411.1_g000030 Rmu_sc0017472.1_g000001 Rmu_sc0017472.1_g000003 Rmu_sc0029876.1_g000001 Rmu_ssc0000480.1_g000024 Rmu_ssc0000480.1_g000036
rosa_roxburghii Rroxscaffold_2G00097070 Rroxscaffold_2G00098540 Rroxscaffold_2G00098560 Rroxscaffold_2G00098570 Rroxscaffold_2G00098650 Rroxscaffold_2G00098710 Rroxscaffold_2G00098740 Rroxscaffold_3G00225830 Rroxscaffold_3G00236870 Rroxscaffold_4G00312240
rosa_rugosa Rorug01G0047000 Rorug02G0372300 Rorug02G0407200 Rorug02G0407300 Rorug02G0408400 Rorug02G0421500 Rorug07G0208900
rosa_samantha Rh2AG380200 Rh2AG465700 Rh2AG465800 Rh2AG466800 Rh2AG467100 Rh2AG467600 Rh2AG480900 Rh2AG481400 Rh2AG481700 Rh2CG073500 Rh2CG452200 Rh2CG453200 Rh2CG453600 Rh2CG454100 Rh2CG467100 Rh2CG467800 Rh2CG468000 Rh2DG403100 Rh2DG487800 Rh2DG487900 Rh2DG488800 Rh2DG502000 Rh2DG502200 Rh2DG502300 Rh2DG503100 Rh3AG319300 Rh4AG227700 Rh4BG371800 Rh5AG327400 Rh5DG350600 Rh7AG349800
rosa_wichuraiana Rw0G010080 Rw2G031110 Rw2G037980 Rw2G037990 Rw2G038000 Rw2G038010 Rw2G038080 Rw2G038100 Rw2G039260 Rw2G039290 Rw2G039470 Rw4G019710 Rw5G030930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1922
Acc65I GGTACC 1 cut(s) 1856
AccB1I GGYRCC 1 cut(s) 1856
AccBSI CCGCTC 1 cut(s) 1537
AccII CGCG 1 cut(s) 1658
AccIII TCCGGA 1 cut(s) 698
AciI CCGC 8 cut(s) 285, 442, 1393, 1537, 1658, 1776, 2084, 2260
AclWI GGATC 3 cut(s) 29, 1145, 1417
AcoI YGGCCR 5 cut(s) 337, 344, 583, 1013, 1741
AcsI RAATTY 3 cut(s) 450, 1649, 2032
AcuI CTGAAG 1 cut(s) 114
AfaI GTAC 8 cut(s) 983, 991, 1391, 1790, 1803, 1826, 1858, 2006
AfiI CCNNNNNNNGG 8 cut(s) 98, 581, 592, 655, 656, 1750, 2018, 2260
AflII CTTAAG 1 cut(s) 11
AgsI TTSAA 9 cut(s) 223, 509, 1049, 1274, 1381, 1639, 1796, 1903, 2018
AhdI GACNNNNNGTC 1 cut(s) 1366
AjnI CCWGG 5 cut(s) 1184, 1598, 1707, 1738, 1859
Alw21I GWGCWC 2 cut(s) 398, 1694
Alw26I GTCTC 1 cut(s) 2085
Alw44I GTGCAC 2 cut(s) 394, 1690
AlwI GGATC 3 cut(s) 29, 1145, 1417
Aor13HI TCCGGA 1 cut(s) 698
AoxI GGCC 8 cut(s) 279, 337, 344, 361, 571, 583, 1013, 1741
ApaLI GTGCAC 2 cut(s) 394, 1690
ApeKI GCWGC 8 cut(s) 287, 1595, 1616, 1778, 1781, 1886, 1913, 2114
ApoI RAATTY 3 cut(s) 450, 1649, 2032
AseI ATTAAT 1 cut(s) 894
Asp700I GAANNNNTTC 1 cut(s) 218
Asp718I GGTACC 1 cut(s) 1856
AspLEI GCGC 2 cut(s) 923, 1923
AspS9I GGNCC 4 cut(s) 362, 572, 696, 830
AsuHPI GGTGA 3 cut(s) 1385, 1529, 2162
AvaII GGWCC 2 cut(s) 696, 830
BaeGI GKGCMC 2 cut(s) 398, 1694
BalI TGGCCA 4 cut(s) 339, 346, 585, 1743
BanI GGYRCC 1 cut(s) 1856
BanII GRGCYC 2 cut(s) 1045, 2242
BbsI GAAGAC 1 cut(s) 131
Bbv12I GWGCWC 2 cut(s) 398, 1694
BbvCI CCTCAGC 3 cut(s) 120, 1212, 2160
BbvI GCAGC 8 cut(s) 274, 1607, 1628, 1765, 1768, 1873, 1925, 2126
BceAI ACGGC 1 cut(s) 1000
BcgI CGANNNNNNTGC 2 cut(s) 1289, 1323
BciT130I CCWGG 5 cut(s) 1186, 1600, 1709, 1740, 1861
BciVI GTATCC 3 cut(s) 948, 1182, 1249
BclI TGATCA 4 cut(s) 625, 1029, 1426, 1762
BcoDI GTCTC 1 cut(s) 2085
BfaI CTAG 3 cut(s) 330, 609, 1323
BfmI CTRYAG 5 cut(s) 46, 432, 775, 1461, 1571
BfrI CTTAAG 1 cut(s) 11
BfuI GTATCC 3 cut(s) 948, 1182, 1249
BisI GCNGC 8 cut(s) 288, 1596, 1617, 1779, 1782, 1887, 1914, 2115
BlpI GCTNAGC 1 cut(s) 1591
BlsI GCNGC 8 cut(s) 289, 1597, 1618, 1780, 1783, 1888, 1915, 2116
Bme1390I CCNGG 5 cut(s) 1186, 1600, 1709, 1740, 1861
Bme18I GGWCC 2 cut(s) 696, 830
BmeRI GACNNNNNGTC 1 cut(s) 1366
BmgT120I GGNCC 4 cut(s) 362, 572, 696, 830
BmiI GGNNCC 5 cut(s) 996, 1530, 1605, 1858, 2265
BmrFI CCNGG 5 cut(s) 1186, 1600, 1709, 1740, 1861
BmrI ACTGGG 1 cut(s) 233
BmsI GCATC 5 cut(s) 763, 1186, 1218, 1488, 1537
BmuI ACTGGG 1 cut(s) 233
BpiI GAAGAC 1 cut(s) 131
BplI GAGNNNNNCTC 2 cut(s) 852, 884
BpmI CTGGAG 1 cut(s) 2196
Bpu10I CCTNAGC 4 cut(s) 120, 1212, 1533, 2160
Bpu1102I GCTNAGC 1 cut(s) 1591
Bsa29I ATCGAT 1 cut(s) 1493
BsaAI YACGTR 1 cut(s) 985
BsaI GGTCTC 1 cut(s) 2085
BsaJI CCNNGG 4 cut(s) 950, 1435, 1599, 1738
BsaWI WCCGGW 2 cut(s) 698, 1192
Bsc4I CCNNNNNNNGG 8 cut(s) 98, 581, 592, 655, 656, 1750, 2018, 2260
Bse118I RCCGGY 1 cut(s) 359
Bse1I ACTGG 3 cut(s) 239, 1264, 2179
Bse3DI GCAATG 1 cut(s) 37
BseAI TCCGGA 1 cut(s) 698
BseBI CCWGG 5 cut(s) 1186, 1600, 1709, 1740, 1861
BseCI ATCGAT 1 cut(s) 1493
BseDI CCNNGG 4 cut(s) 950, 1435, 1599, 1738
BseGI GGATG 9 cut(s) 259, 645, 754, 789, 946, 1112, 1222, 1227, 1445
BseLI CCNNNNNNNGG 8 cut(s) 98, 581, 592, 655, 656, 1750, 2018, 2260
BseMI GCAATG 1 cut(s) 37
BseMII CTCAG 7 cut(s) 111, 1203, 1364, 1524, 1659, 2150, 2174
BseNI ACTGG 3 cut(s) 239, 1264, 2179
BseSI GKGCMC 2 cut(s) 398, 1694
BseXI GCAGC 8 cut(s) 274, 1607, 1628, 1765, 1768, 1873, 1925, 2126
BsgI GTGCAG 4 cut(s) 514, 1767, 1932, 2133
Bsh1236I CGCG 1 cut(s) 1658
BshFI GGCC 8 cut(s) 281, 339, 346, 363, 573, 585, 1015, 1743
BshNI GGYRCC 1 cut(s) 1856
BshVI ATCGAT 1 cut(s) 1493
BsiHKAI GWGCWC 2 cut(s) 398, 1694
BsiSI CCGG 3 cut(s) 360, 699, 1193
BslI CCNNNNNNNGG 8 cut(s) 98, 581, 592, 655, 656, 1750, 2018, 2260
BsmAI GTCTC 1 cut(s) 2085
BsmI GAATGC 1 cut(s) 201
BsnI GGCC 8 cut(s) 281, 339, 346, 363, 573, 585, 1015, 1743
Bso31I GGTCTC 1 cut(s) 2085
Bsp1286I GDGCHC 4 cut(s) 398, 1045, 1694, 2242
Bsp13I TCCGGA 1 cut(s) 698
Bsp1407I TGTACA 1 cut(s) 1801
Bsp143I GATC 8 cut(s) 34, 625, 1029, 1150, 1409, 1426, 1762, 2027
Bsp1720I GCTNAGC 1 cut(s) 1591
Bsp19I CCATGG 1 cut(s) 1435
BspACI CCGC 8 cut(s) 285, 442, 1393, 1537, 1658, 1776, 2084, 2260
BspANI GGCC 8 cut(s) 281, 339, 346, 363, 573, 585, 1015, 1743
BspCNI CTCAG 7 cut(s) 112, 1204, 1363, 1525, 1658, 2149, 2173
BspDI ATCGAT 1 cut(s) 1493
BspEI TCCGGA 1 cut(s) 698
BspFNI CGCG 1 cut(s) 1658
BspHI TCATGA 1 cut(s) 2184
BspLI GGNNCC 5 cut(s) 996, 1530, 1605, 1858, 2265
BspMAI CTGCAG 1 cut(s) 1465
BspPI GGATC 3 cut(s) 29, 1145, 1417
BspQI GCTCTTC 1 cut(s) 105
BspT107I GGYRCC 1 cut(s) 1856
BspTI CTTAAG 1 cut(s) 11
BspTNI GGTCTC 1 cut(s) 2085
BsrBI CCGCTC 1 cut(s) 1537
BsrDI GCAATG 1 cut(s) 37
BsrFI RCCGGY 1 cut(s) 359
BsrGI TGTACA 1 cut(s) 1801
BsrI ACTGG 3 cut(s) 239, 1264, 2179
BssAI RCCGGY 1 cut(s) 359
BssECI CCNNGG 4 cut(s) 950, 1435, 1599, 1738
BssMI GATC 8 cut(s) 34, 625, 1029, 1150, 1409, 1426, 1762, 2027
BssT1I CCWWGG 2 cut(s) 950, 1435
Bst2UI CCWGG 5 cut(s) 1186, 1600, 1709, 1740, 1861
Bst4CI ACNGT 4 cut(s) 424, 779, 1251, 1788
Bst6I CTCTTC 3 cut(s) 105, 1005, 1749
BstAFI CTTAAG 1 cut(s) 11
BstAUI TGTACA 1 cut(s) 1801
BstBAI YACGTR 1 cut(s) 985
BstDEI CTNAG 9 cut(s) 120, 206, 1212, 1350, 1533, 1591, 1645, 2136, 2160
BstDSI CCRYGG 1 cut(s) 1435
BstENI CCTNNNNNAGG 2 cut(s) 96, 2016
BstF5I GGATG 9 cut(s) 259, 645, 754, 789, 946, 1112, 1222, 1227, 1445
BstFNI CGCG 1 cut(s) 1658
BstHHI GCGC 2 cut(s) 923, 1923
BstKTI GATC 8 cut(s) 37, 628, 1032, 1153, 1412, 1429, 1765, 2030
BstMAI GTCTC 1 cut(s) 2085
BstMBI GATC 8 cut(s) 34, 625, 1029, 1150, 1409, 1426, 1762, 2027
BstMWI GCNNNNNNNGC 9 cut(s) 287, 326, 356, 1274, 1283, 1392, 1616, 1709, 2081
BstNI CCWGG 5 cut(s) 1186, 1600, 1709, 1740, 1861
BstNSI RCATGY 5 cut(s) 796, 1067, 1132, 1390, 2200
BstSCI CCNGG 5 cut(s) 1184, 1598, 1707, 1738, 1859
BstSFI CTRYAG 5 cut(s) 46, 432, 775, 1461, 1571
BstSLI GKGCMC 2 cut(s) 398, 1694
BstSNI TACGTA 1 cut(s) 985
BstUI CGCG 1 cut(s) 1658
BstV1I GCAGC 8 cut(s) 274, 1607, 1628, 1765, 1768, 1873, 1925, 2126
BstV2I GAAGAC 1 cut(s) 131
BstX2I RGATCY 1 cut(s) 1150
BstXI CCANNNNNNTGG 2 cut(s) 488, 726
BstYI RGATCY 1 cut(s) 1150
Bsu15I ATCGAT 1 cut(s) 1493
BsuI GTATCC 3 cut(s) 948, 1182, 1249
BsuRI GGCC 8 cut(s) 281, 339, 346, 363, 573, 585, 1015, 1743
BsuTUI ATCGAT 1 cut(s) 1493
BtgI CCRYGG 1 cut(s) 1435
BtsCI GGATG 9 cut(s) 259, 645, 754, 789, 946, 1112, 1222, 1227, 1445
BtsI GCAGTG 3 cut(s) 640, 916, 1458
BtsIMutI CAGTG 7 cut(s) 246, 640, 916, 1256, 1458, 1784, 2172
CciI TCATGA 1 cut(s) 2184
CfoI GCGC 2 cut(s) 923, 1923
Cfr10I RCCGGY 1 cut(s) 359
Cfr13I GGNCC 4 cut(s) 362, 572, 696, 830
ClaI ATCGAT 1 cut(s) 1493
CseI GACGC 1 cut(s) 899
CsiI ACCWGGT 1 cut(s) 1859
Csp6I GTAC 8 cut(s) 982, 990, 1390, 1789, 1802, 1825, 1857, 2005
CviQI GTAC 8 cut(s) 982, 990, 1390, 1789, 1802, 1825, 1857, 2005
DdeI CTNAG 9 cut(s) 120, 206, 1212, 1350, 1533, 1591, 1645, 2136, 2160
DpnI GATC 8 cut(s) 36, 627, 1031, 1152, 1411, 1428, 1764, 2029
DpnII GATC 8 cut(s) 34, 625, 1029, 1150, 1409, 1426, 1762, 2027
DraI TTTAAA 2 cut(s) 1171, 1473
DriI GACNNNNNGTC 1 cut(s) 1366
EaeI YGGCCR 5 cut(s) 337, 344, 583, 1013, 1741
Eam1104I CTCTTC 3 cut(s) 105, 1005, 1749
Eam1105I GACNNNNNGTC 1 cut(s) 1366
EarI CTCTTC 3 cut(s) 105, 1005, 1749
EciI GGCGGA 1 cut(s) 2099
Eco105I TACGTA 1 cut(s) 985
Eco130I CCWWGG 2 cut(s) 950, 1435
Eco24I GRGCYC 2 cut(s) 1045, 2242
Eco31I GGTCTC 1 cut(s) 2085
Eco47I GGWCC 2 cut(s) 696, 830
Eco57I CTGAAG 1 cut(s) 114
EcoNI CCTNNNNNAGG 2 cut(s) 96, 2016
EcoO109I RGGNCCY 1 cut(s) 572
EcoRI GAATTC 1 cut(s) 1649
EcoRII CCWGG 5 cut(s) 1184, 1598, 1707, 1738, 1859
EcoT14I CCWWGG 2 cut(s) 950, 1435
EcoT22I ATGCAT 2 cut(s) 756, 1130
EcoT38I GRGCYC 2 cut(s) 1045, 2242
ErhI CCWWGG 2 cut(s) 950, 1435
FalI AAGNNNNNCTT 4 cut(s) 123, 155, 1266, 1298
FauI CCCGC 1 cut(s) 2253
FauNDI CATATG 1 cut(s) 1852
FbaI TGATCA 4 cut(s) 625, 1029, 1426, 1762
Fnu4HI GCNGC 8 cut(s) 288, 1596, 1617, 1779, 1782, 1887, 1914, 2115
FokI GGATG 9 cut(s) 266, 632, 741, 796, 933, 1119, 1229, 1234, 1452
FriOI GRGCYC 2 cut(s) 1045, 2242
Fsp4HI GCNGC 8 cut(s) 288, 1596, 1617, 1779, 1782, 1887, 1914, 2115
FspBI CTAG 3 cut(s) 330, 609, 1323
FspI TGCGCA 1 cut(s) 1922
GlaI GCGC 2 cut(s) 922, 1922
GluI GCNGC 8 cut(s) 288, 1596, 1617, 1779, 1782, 1887, 1914, 2115
GsuI CTGGAG 1 cut(s) 2196
HaeIII GGCC 8 cut(s) 281, 339, 346, 363, 573, 585, 1015, 1743
HapII CCGG 3 cut(s) 360, 699, 1193
HgaI GACGC 1 cut(s) 899
HhaI GCGC 2 cut(s) 923, 1923
Hin6I GCGC 2 cut(s) 921, 1921
HinP1I GCGC 2 cut(s) 921, 1921
HincII GTYRAC 1 cut(s) 1247
HindII GTYRAC 1 cut(s) 1247
HindIII AAGCTT 2 cut(s) 8, 1275
HinfI GANTC 2 cut(s) 619, 2068
HpaII CCGG 3 cut(s) 360, 699, 1193
HphI GGTGA 3 cut(s) 1385, 1529, 2162
Hpy166II GTNNAC 8 cut(s) 307, 396, 790, 931, 1247, 1416, 1692, 2041
Hpy188I TCNGA 5 cut(s) 477, 1426, 1453, 1648, 2139
Hpy188III TCNNGA 9 cut(s) 623, 699, 1033, 1046, 1342, 1430, 1951, 2015, 2185
Hpy8I GTNNAC 8 cut(s) 307, 396, 790, 931, 1247, 1416, 1692, 2041
Hpy99I CGWCG 2 cut(s) 915, 1372
HpyCH4III ACNGT 4 cut(s) 424, 779, 1251, 1788
HpyCH4IV ACGT 3 cut(s) 984, 1683, 2007
HpyF10VI GCNNNNNNNGC 9 cut(s) 287, 326, 356, 1274, 1283, 1392, 1616, 1709, 2081
HpyF3I CTNAG 9 cut(s) 120, 206, 1212, 1350, 1533, 1591, 1645, 2136, 2160
HpySE526I ACGT 3 cut(s) 984, 1683, 2007
HspAI GCGC 2 cut(s) 921, 1921
Kpn2I TCCGGA 1 cut(s) 698
KpnI GGTACC 1 cut(s) 1860
KroI GCCGGC 1 cut(s) 359
KroNI GCCGGC 1 cut(s) 361
Ksp22I TGATCA 4 cut(s) 625, 1029, 1426, 1762
Kzo9I GATC 8 cut(s) 34, 625, 1029, 1150, 1409, 1426, 1762, 2027
LguI GCTCTTC 1 cut(s) 105
LmnI GCTCC 5 cut(s) 994, 1060, 1528, 1613, 2177
Lsp1109I GCAGC 8 cut(s) 274, 1607, 1628, 1765, 1768, 1873, 1925, 2126
LweI GCATC 5 cut(s) 763, 1186, 1218, 1488, 1537
MabI ACCWGGT 1 cut(s) 1859
MaeI CTAG 3 cut(s) 330, 609, 1323
MaeII ACGT 3 cut(s) 984, 1683, 2007
MaeIII GTNAC 6 cut(s) 179, 458, 637, 914, 1051, 1373
MalI GATC 8 cut(s) 36, 627, 1031, 1152, 1411, 1428, 1764, 2029
MbiI CCGCTC 1 cut(s) 1537
MboI GATC 8 cut(s) 34, 625, 1029, 1150, 1409, 1426, 1762, 2027
MfeI CAATTG 1 cut(s) 1906
MflI RGATCY 1 cut(s) 1150
MhlI GDGCHC 4 cut(s) 398, 1045, 1694, 2242
MlsI TGGCCA 4 cut(s) 339, 346, 585, 1743
MluNI TGGCCA 4 cut(s) 339, 346, 585, 1743
Mox20I TGGCCA 4 cut(s) 339, 346, 585, 1743
Mph1103I ATGCAT 2 cut(s) 756, 1130
MroI TCCGGA 1 cut(s) 698
MroNI GCCGGC 1 cut(s) 359
MroXI GAANNNNTTC 1 cut(s) 218
MscI TGGCCA 4 cut(s) 339, 346, 585, 1743
MslI CAYNNNNRTG 3 cut(s) 551, 633, 797
Msp20I TGGCCA 4 cut(s) 339, 346, 585, 1743
MspA1I CMGCKG 3 cut(s) 287, 350, 1778
MspCI CTTAAG 1 cut(s) 11
MspI CCGG 3 cut(s) 360, 699, 1193
MspR9I CCNGG 5 cut(s) 1186, 1600, 1709, 1740, 1861
MunI CAATTG 1 cut(s) 1906
Mva1269I GAATGC 1 cut(s) 201
MvaI CCWGG 5 cut(s) 1186, 1600, 1709, 1740, 1861
MvnI CGCG 1 cut(s) 1658
MwoI GCNNNNNNNGC 9 cut(s) 287, 326, 356, 1274, 1283, 1392, 1616, 1709, 2081
NaeI GCCGGC 1 cut(s) 361
NcoI CCATGG 1 cut(s) 1435
NdeI CATATG 1 cut(s) 1852
NdeII GATC 8 cut(s) 34, 625, 1029, 1150, 1409, 1426, 1762, 2027
NgoMIV GCCGGC 1 cut(s) 359
NlaIV GGNNCC 5 cut(s) 996, 1530, 1605, 1858, 2265
NmuCI GTSAC 3 cut(s) 179, 914, 1373
NsbI TGCGCA 1 cut(s) 1922
NsiI ATGCAT 2 cut(s) 756, 1130
NspI RCATGY 5 cut(s) 796, 1067, 1132, 1390, 2200
PaeI GCATGC 2 cut(s) 1132, 2200
PagI TCATGA 1 cut(s) 2184
PciSI GCTCTTC 1 cut(s) 105
PctI GAATGC 1 cut(s) 201
PdiI GCCGGC 1 cut(s) 361
PdmI GAANNNNTTC 1 cut(s) 218
PfeI GAWTC 2 cut(s) 619, 2068
PflFI GACNNNGTC 1 cut(s) 1337
PkrI GCNGC 8 cut(s) 289, 1597, 1618, 1780, 1783, 1888, 1915, 2116
Ppu21I YACGTR 1 cut(s) 985
PshBI ATTAAT 1 cut(s) 894
Psp6I CCWGG 5 cut(s) 1184, 1598, 1707, 1738, 1859
PspGI CCWGG 5 cut(s) 1184, 1598, 1707, 1738, 1859
PspN4I GGNNCC 5 cut(s) 996, 1530, 1605, 1858, 2265
PspPI GGNCC 4 cut(s) 362, 572, 696, 830
PstI CTGCAG 1 cut(s) 1465
PsuI RGATCY 1 cut(s) 1150
PsyI GACNNNGTC 1 cut(s) 1337
PvuII CAGCTG 1 cut(s) 350
RsaI GTAC 8 cut(s) 983, 991, 1391, 1790, 1803, 1826, 1858, 2006
RsaNI GTAC 8 cut(s) 982, 990, 1390, 1789, 1802, 1825, 1857, 2005
RseI CAYNNNNRTG 3 cut(s) 551, 633, 797
SapI GCTCTTC 1 cut(s) 105
SatI GCNGC 8 cut(s) 288, 1596, 1617, 1779, 1782, 1887, 1914, 2115
Sau3AI GATC 8 cut(s) 34, 625, 1029, 1150, 1409, 1426, 1762, 2027
Sau96I GGNCC 4 cut(s) 362, 572, 696, 830
ScrFI CCNGG 5 cut(s) 1186, 1600, 1709, 1740, 1861
SduI GDGCHC 4 cut(s) 398, 1045, 1694, 2242
SexAI ACCWGGT 1 cut(s) 1859
SfaNI GCATC 5 cut(s) 763, 1186, 1218, 1488, 1537
SfcI CTRYAG 5 cut(s) 46, 432, 775, 1461, 1571
SinI GGWCC 2 cut(s) 696, 830
SmiMI CAYNNNNRTG 3 cut(s) 551, 633, 797
SmlI CTYRAG 1 cut(s) 11
SmoI CTYRAG 1 cut(s) 11
SnaBI TACGTA 1 cut(s) 985
SphI GCATGC 2 cut(s) 1132, 2200
SsiI CCGC 8 cut(s) 285, 442, 1393, 1537, 1658, 1776, 2084, 2260
SspI AATATT 1 cut(s) 2224
SspMI CTAG 3 cut(s) 330, 609, 1323
StyD4I CCNGG 5 cut(s) 1184, 1598, 1707, 1738, 1859
StyI CCWWGG 2 cut(s) 950, 1435
TaaI ACNGT 4 cut(s) 424, 779, 1251, 1788
TaiI ACGT 3 cut(s) 987, 1686, 2010
TaqI TCGA 3 cut(s) 674, 1493, 1545
TatI WGTACW 1 cut(s) 1801
TfiI GAWTC 2 cut(s) 619, 2068
TscAI CASTG 7 cut(s) 246, 640, 923, 1256, 1465, 1791, 2179
TseFI GTSAC 3 cut(s) 179, 914, 1373
TseI GCWGC 8 cut(s) 287, 1595, 1616, 1778, 1781, 1886, 1913, 2114
Tsp45I GTSAC 3 cut(s) 179, 914, 1373
TspGWI ACGGA 2 cut(s) 1007, 1356
TspRI CASTG 7 cut(s) 246, 640, 923, 1256, 1465, 1791, 2179
Tth111I GACNNNGTC 1 cut(s) 1337
Vha464I CTTAAG 1 cut(s) 11
VneI GTGCAC 2 cut(s) 394, 1690
VpaK11BI GGWCC 2 cut(s) 696, 830
VspI ATTAAT 1 cut(s) 894
XagI CCTNNNNNAGG 2 cut(s) 96, 2016
XapI RAATTY 3 cut(s) 450, 1649, 2032
XceI RCATGY 5 cut(s) 796, 1067, 1132, 1390, 2200
XcmI CCANNNNNNNNNTGG 2 cut(s) 1108, 1958
XmnI GAANNNNTTC 1 cut(s) 218
XspI CTAG 3 cut(s) 330, 609, 1323
Zsp2I ATGCAT 2 cut(s) 756, 1130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.