RLG00000020358

Belongs to the terpene cyclase mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000173
Physical Location & Seq
Forward (+)
64155868 .. 64164765
8898 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020358

Sequence Viewer

Length: 2295 bp
ATGTGGAAGCTTAAGGTTGCAGAAGCTGGGGACTCCTCAGCTTACATTTACAGCACAAACAACTTTGTCGGAAGGCAGACATGGGAGTTTGATCCCGACGCAGGAACTCCTGAAGAGCGAGCTGAGGTTGAAGAGGCCCGTCTTCATTTCTACAACAACCGCTATCAGGTCAAGCCCAGTGGTGATCTGCTTTGGCGTATGCAGTTCCTAAGGGAGAAGAAGTTCAAACAGACAATTCCTCCAGTAAAAATTGAAGATGGCGAGGTTATCACACATGAAAAAACCACAAATGCACTAAGAAGGTCTGTCCACTTCTTCTCAGCATTGCAGGCCAGCGATGGCCATTGGCCTGCGGAAAATGCCGGCCCTTTGTTTTTCCTTCCTCCCTTGGTCATATGTATGTACATTACTGGCCATCTTAACAGTGTATTCCCTGAAGAGCATCGCAAAGAAATTCTGCGTTACATATACTATCATCAGAATGAAGATGGTGGTTGGGGACTACACGTCGAAGGTCACAGCACCATGTTCAGCACAGCTCTCAGCTACATTTGTATGCGTATTCTCGGAGAAGGACCTGATGATGGTGGCCAAGACAAAGCTTGTCCAAGAGCAAGAAAGTGGATTCATGATCATGGTGGTGTCACACACATACCCTCTTGGGGAAAGACTTGGCTTTCGATACTTGGTTTGTTTGATTGGTCTGGAAGCAACCCTATGCCCCCAGAGTTTTGGATTCTTCCTTCGTTTCTTCCTATGCATCCAGCAAAAATGTGGTGCTATTGTAGGATGGTTTACATGCCAATGTCATACCTATATGGAAAAAGGTTTGTTGGCTCAATTACACCTCTCATTCTTCAATTGAGGGAAGAACTTTTTATTCAACCTTACAATGAAATCAATTGGAAGAAAGTACGCCATCTATGTGCCAAAGAGGACATCTACTATCCTCATCCTTGGATACAGGATCTCCTTTGGGATAGCCTCTACATTTCCACAGAGCCTATTCTTACTCGTTGGCCCTTCAACAAGTTGATCAGAGAAAAGGCTCTTCAAGTGACGATGAAGCATATTCATTATGAAGATGAGAACAGTCGATACATTACCATTGGGTGTGTGGAAAAGGTGTTATGTATGCTTGCCTGTTGGGTTGAAGATCCAAATGGGGACTATTTCAAAAAGCATCTGGCAAGGGTCCCGGATTATTTATGGGTTGCTGAAGATGGAATGAAGATGCAAAGTTTTGGCAGTCAGGAGTGGGATACTGGTTTTGCCATTCAAACTTTGCTTGCTACTAATCTAACCGACGAAATTGAACCAACGCTCGCTAGAGGACATGACTTCATAAAGAAATCTCAGGTCAAAGACAACCCATCTGGTGACTTCAAAAGCATGCACCGCCACATTTCCAAAGGATCGTGGACTTTCTCCGATCAAGATCATGGATGGCAAGTTTCTGATTGCACTGCAGAAGGCTTAAAGTGTTGCCTTCTATTGTCGATGATGCCATCAGAGATAGTTGGTGAAGAAATGGAACCTGAGCGCTTATACGATTCTGTCAATCTTCTACTTTCCCTACAGAGCAAGAATGGTGGTTTATCAGCCTGGGAACCAGCAGGGGCAGCAGAGTGGTTAGAATTCTTCGCAGACATAGTAATTGAGCATGAATATGTTGAGTGCACTTCATCTGCAATCCAGGCTTTAGTACTGTTTAAGAAGCTACACCCTGGACACAAGAAGAAAGAGATTGAACATTTCATCACCAATGCTATACACTACCTTGAAAATATACAAATGCCGGATGGTTCATGGTATGGAAACTGGGGAGTTTGCTTCACATATGGTACCTGGTTTGCAGTAGGAGGATTGGCAGCAGCTGGCAAAACTTTCAGCACTTGTGCAGCCATGCGCAAAGCGGTTAGTTTTCTCCTCACAACGCAGAGAGAGAATGGTGGTTGGGGAGAGAGCTATCTTTCATGTCCGCAAAAGACATACATTCCGCTCGAAGGAAATCGATCCAATTTAGTACATACTGCTTGGGCTATGATGGGTCTCATTCATGCGGGACAGGCAGAAAGAGACCCAACACCTCTTCATCGTGCAGCAAAGTTTATAATTAATTCTCAACTGGAAAATGGTGATTTTCCCCAGCAGGAAATCACTGGAGTCTTCATGAAGAACTGCATGCTACATTATGCAGCTTATAGAAATATATACCCACTCTGGGCTCTTGCAGAATACCGCAAGCGGGTTCCATTGCCTTCCAAGGCCAGAATTGTTCACCCCGTTATGTAG

Protein Analysis

765

Amino Acids

87.85

Weight (kDa)

6.25

Isoelectric Point (pI)

49.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQHop_cyclase_N PF13249 100 - 360 3.5e-35 Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C PF13243 419 - 749 1.9e-51 Squalene-hopene cyclase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66960 AT1G66960 AT1G66960 AT1G66960 AT1G78950 AT1G78950 AT1G78955 AT1G78955 AT1G78955 AT1G78955 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78970 AT1G78970 AT1G78970
fragaria_vesca FvH4_5g29480 FvH4_6g36450 FvH4_6g36450 FvH4_6g36500 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g37620
malus_domestica MD03G1089600.v1.1 MD09G1167700.v1.1 MD09G1168200.v1.1 MD09G1168300.v1.1 MD11G1098600.v1.1 MD17G1158300.v1.1 MD17G1158700.v1.1 MD17G1159500.v1.1 MD17G1160500.v1.1 MD17G1182500.v1.1 MD17G1182700.v1.1 MD17G1245800.v1.1 MD17G1246300.v1.1 MD17G1246600.v1.1
prunus_persica Prupe.3G025700_v2.0.a1 Prupe.3G025700_v2.0.a1 Prupe.3G025800_v2.0.a1 Prupe.3G025900_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026400_v2.0.a1 Prupe.3G026500_v2.0.a1 Prupe.3G026700_v2.0.a1 Prupe.3G026800_v2.0.a1
pyrus_communis pycom03g07120 pycom09g08520 pycom09g08590 pycom09g08610 pycom10g10070 pycom11g08310 pycom16g19880 pycom17g15190 pycom17g15300
rosa_chinensis RchiOBHm_Chr2g0136221 RchiOBHm_Chr2g0148451 RchiOBHm_Chr2g0148501 RchiOBHm_Chr2g0148521 RchiOBHm_Chr2g0148661 RchiOBHm_Chr2g0148701 RchiOBHm_Chr2g0148811 RchiOBHm_Chr2g0148941 RchiOBHm_Chr2g0148981 RchiOBHm_Chr2g0150451 RchiOBHm_Chr2g0150521 RchiOBHm_Chr2g0150611 RchiOBHm_Chr4g0420831 RchiOBHm_Chr5g0049791 RchiOBHm_Chr5g0049801
rosa_laevigata RLG00000007735 RLG00000020351 RLG00000020352 RLG00000020357 RLG00000020358 RLG00000020360 RLG00000029093 RLG00000030686
rosa_multiflora Rmu_co8274513.1_g000001 Rmu_sc0000048.1_g000003 Rmu_sc0000048.1_g000033 Rmu_sc0000048.1_g000039 Rmu_sc0001397.1_g000011 Rmu_sc0001998.1_g000039 Rmu_sc0002070.1_g000055 Rmu_sc0002082.1_g000052 Rmu_sc0003181.1_g000010 Rmu_sc0005104.1_g000005 Rmu_sc0006758.1_g000002 Rmu_sc0008411.1_g000029 Rmu_sc0008411.1_g000030 Rmu_sc0017472.1_g000001 Rmu_sc0017472.1_g000003 Rmu_sc0029876.1_g000001 Rmu_ssc0000480.1_g000024 Rmu_ssc0000480.1_g000036
rosa_roxburghii Rroxscaffold_2G00097070 Rroxscaffold_2G00098540 Rroxscaffold_2G00098560 Rroxscaffold_2G00098570 Rroxscaffold_2G00098650 Rroxscaffold_2G00098710 Rroxscaffold_2G00098740 Rroxscaffold_3G00225830 Rroxscaffold_3G00236870 Rroxscaffold_4G00312240
rosa_rugosa Rorug01G0047000 Rorug02G0372300 Rorug02G0407200 Rorug02G0407300 Rorug02G0408400 Rorug02G0421500 Rorug07G0208900
rosa_samantha Rh2AG380200 Rh2AG465700 Rh2AG465800 Rh2AG466800 Rh2AG467100 Rh2AG467600 Rh2AG480900 Rh2AG481400 Rh2AG481700 Rh2CG073500 Rh2CG452200 Rh2CG453200 Rh2CG453600 Rh2CG454100 Rh2CG467100 Rh2CG467800 Rh2CG468000 Rh2DG403100 Rh2DG487800 Rh2DG487900 Rh2DG488800 Rh2DG502000 Rh2DG502200 Rh2DG502300 Rh2DG503100 Rh3AG319300 Rh4AG227700 Rh4BG371800 Rh5AG327400 Rh5DG350600 Rh7AG349800
rosa_wichuraiana Rw0G010080 Rw2G031110 Rw2G037980 Rw2G037990 Rw2G038000 Rw2G038010 Rw2G038080 Rw2G038100 Rw2G039260 Rw2G039290 Rw2G039470 Rw4G019710 Rw5G030930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2114
Acc16I TGCGCA 1 cut(s) 1910
Acc65I GGTACC 1 cut(s) 1844
AccB1I GGYRCC 1 cut(s) 1844
AccBSI CCGCTC 1 cut(s) 2002
AciI CCGC 9 cut(s) 160, 353, 1399, 1916, 1982, 2000, 2063, 2242, 2248
AclWI GGATC 5 cut(s) 86, 975, 1151, 1423, 2010
AcoI YGGCCR 3 cut(s) 340, 412, 589
AcsI RAATTY 2 cut(s) 453, 1637
AcuI CTGAAG 3 cut(s) 132, 456, 1239
AfaI GTAC 5 cut(s) 404, 915, 1707, 1846, 2028
AfeI AGCGCT 1 cut(s) 1544
AfiI CCNNNNNNNGG 7 cut(s) 101, 166, 584, 662, 2006, 2224, 2248
AflII CTTAAG 1 cut(s) 11
AflIII ACRYGT 1 cut(s) 505
AhdI GACNNNNNGTC 1 cut(s) 506
AjiI CACGTC 1 cut(s) 508
AjnI CCWGG 4 cut(s) 1604, 1695, 1726, 1847
AloI GAACNNNNNNTCC 2 cut(s) 206, 238
Alw21I GWGCWC 1 cut(s) 1682
Alw26I GTCTC 2 cut(s) 2057, 2073
Alw44I GTGCAC 1 cut(s) 1678
AlwI GGATC 5 cut(s) 86, 975, 1151, 1423, 2010
AlwNI CAGNNNCTG 2 cut(s) 26, 1877
Aor51HI AGCGCT 1 cut(s) 1544
AoxI GGCC 9 cut(s) 135, 330, 340, 347, 364, 412, 589, 1019, 2268
ApaLI GTGCAC 1 cut(s) 1678
ApeKI GCWGC 6 cut(s) 1622, 1871, 1874, 1901, 2102, 2198
ApoI RAATTY 2 cut(s) 453, 1637
ArsI GACNNNNNNTTYG 2 cut(s) 661, 693
AseI ATTAAT 1 cut(s) 2118
Asp700I GAANNNNTTC 1 cut(s) 221
Asp718I GGTACC 1 cut(s) 1844
AspLEI GCGC 2 cut(s) 1545, 1911
AspS9I GGNCC 5 cut(s) 136, 365, 575, 1020, 1195
AsuC2I CCSGG 1 cut(s) 1199
AsuHPI GGTGA 6 cut(s) 194, 1391, 1535, 1753, 2150, 2273
AvaII GGWCC 2 cut(s) 575, 1195
AxyI CCTNAGG 1 cut(s) 209
BaeGI GKGCMC 1 cut(s) 1682
BalI TGGCCA 3 cut(s) 342, 414, 591
BanI GGYRCC 1 cut(s) 1844
BanII GRGCYC 1 cut(s) 2230
BbsI GAAGAC 2 cut(s) 134, 2161
Bbv12I GWGCWC 1 cut(s) 1682
BbvCI CCTCAGC 2 cut(s) 37, 123
BbvI GCAGC 6 cut(s) 1634, 1883, 1886, 1913, 2114, 2210
BciT130I CCWGG 4 cut(s) 1606, 1697, 1728, 1849
BciVI GTATCC 2 cut(s) 954, 1255
BclI TGATCA 2 cut(s) 631, 1035
BcnI CCSGG 1 cut(s) 1199
BcoDI GTCTC 2 cut(s) 2057, 2073
BfaI CTAG 1 cut(s) 1329
BfmI CTRYAG 2 cut(s) 1467, 1577
BfoI RGCGCY 1 cut(s) 1546
BfrI CTTAAG 1 cut(s) 11
BfuI GTATCC 2 cut(s) 954, 1255
BisI GCNGC 6 cut(s) 1623, 1872, 1875, 1902, 2103, 2199
BlsI GCNGC 6 cut(s) 1624, 1873, 1876, 1903, 2104, 2200
BmcAI AGTACT 1 cut(s) 1707
Bme1390I CCNGG 5 cut(s) 1199, 1606, 1697, 1728, 1849
Bme18I GGWCC 2 cut(s) 575, 1195
BmeRI GACNNNNNGTC 1 cut(s) 506
BmgBI CACGTC 1 cut(s) 508
BmgT120I GGNCC 5 cut(s) 136, 365, 575, 1020, 1195
BmiI GGNNCC 6 cut(s) 1196, 1197, 1536, 1611, 1846, 2253
BmrFI CCNGG 5 cut(s) 1199, 1606, 1697, 1728, 1849
BmrI ACTGGG 2 cut(s) 171, 1831
BmsI GCATC 5 cut(s) 451, 769, 1192, 1224, 1494
BmuI ACTGGG 2 cut(s) 171, 1831
BpiI GAAGAC 2 cut(s) 134, 2161
BpmI CTGGAG 2 cut(s) 225, 2184
Bpu10I CCTNAGC 3 cut(s) 37, 123, 1539
BpuMI CCSGG 1 cut(s) 1199
Bsa29I ATCGAT 1 cut(s) 2014
BsaBI GATNNNNATC 1 cut(s) 1437
BsaI GGTCTC 2 cut(s) 2057, 2073
BsaJI CCNNGG 5 cut(s) 387, 956, 1605, 1726, 2265
BsaXI ACNNNNNCTCC 6 cut(s) 206, 223, 236, 253, 954, 984
Bsc4I CCNNNNNNNGG 7 cut(s) 101, 166, 584, 662, 2006, 2224, 2248
Bse118I RCCGGY 1 cut(s) 362
Bse1I ACTGG 7 cut(s) 177, 242, 415, 1270, 1826, 2133, 2167
Bse21I CCTNAGG 1 cut(s) 209
Bse3DI GCAATG 2 cut(s) 323, 2255
Bse8I GATNNNNATC 1 cut(s) 1437
BseBI CCWGG 4 cut(s) 1606, 1697, 1728, 1849
BseCI ATCGAT 1 cut(s) 2014
BseDI CCNNGG 5 cut(s) 387, 956, 1605, 1726, 2265
BseGI GGATG 5 cut(s) 760, 795, 952, 1451, 1807
BseJI GATNNNNATC 1 cut(s) 1437
BseLI CCNNNNNNNGG 7 cut(s) 101, 166, 584, 662, 2006, 2224, 2248
BseMI GCAATG 2 cut(s) 323, 2255
BseMII CTCAG 6 cut(s) 51, 114, 333, 556, 1370, 1530
BseNI ACTGG 7 cut(s) 177, 242, 415, 1270, 1826, 2133, 2167
BseRI GAGGAG 2 cut(s) 25, 1919
BseSI GKGCMC 1 cut(s) 1682
BseXI GCAGC 6 cut(s) 1634, 1883, 1886, 1913, 2114, 2210
BseYI CCCAGC 2 cut(s) 26, 2148
BsgI GTGCAG 2 cut(s) 1920, 2121
BshFI GGCC 9 cut(s) 137, 332, 342, 349, 366, 414, 591, 1021, 2270
BshNI GGYRCC 1 cut(s) 1844
BshVI ATCGAT 1 cut(s) 2014
BsiHKAI GWGCWC 1 cut(s) 1682
BsiSI CCGG 3 cut(s) 363, 1199, 1799
BslFI GGGAC 5 cut(s) 44, 513, 1181, 1181, 2079
BslI CCNNNNNNNGG 7 cut(s) 101, 166, 584, 662, 2006, 2224, 2248
BsmAI GTCTC 2 cut(s) 2057, 2073
BsmFI GGGAC 5 cut(s) 44, 513, 1181, 1181, 2079
BsnI GGCC 9 cut(s) 137, 332, 342, 349, 366, 414, 591, 1021, 2270
Bso31I GGTCTC 2 cut(s) 2057, 2073
Bsp1286I GDGCHC 2 cut(s) 1682, 2230
Bsp1407I TGTACA 1 cut(s) 402
BspACI CCGC 9 cut(s) 160, 353, 1399, 1916, 1982, 2000, 2063, 2242, 2248
BspANI GGCC 9 cut(s) 137, 332, 342, 349, 366, 414, 591, 1021, 2270
BspCNI CTCAG 6 cut(s) 50, 115, 332, 555, 1369, 1531
BspDI ATCGAT 1 cut(s) 2014
BspHI TCATGA 2 cut(s) 628, 2172
BspLI GGNNCC 6 cut(s) 1196, 1197, 1536, 1611, 1846, 2253
BspMAI CTGCAG 1 cut(s) 1471
BspPI GGATC 5 cut(s) 86, 975, 1151, 1423, 2010
BspQI GCTCTTC 3 cut(s) 108, 432, 1056
BspT107I GGYRCC 1 cut(s) 1844
BspTI CTTAAG 1 cut(s) 11
BspTNI GGTCTC 2 cut(s) 2057, 2073
BsrBI CCGCTC 1 cut(s) 2002
BsrDI GCAATG 2 cut(s) 323, 2255
BsrFI RCCGGY 1 cut(s) 362
BsrGI TGTACA 1 cut(s) 402
BsrI ACTGG 7 cut(s) 177, 242, 415, 1270, 1826, 2133, 2167
BssAI RCCGGY 1 cut(s) 362
BssECI CCNNGG 5 cut(s) 387, 956, 1605, 1726, 2265
BssT1I CCWWGG 3 cut(s) 387, 956, 2265
Bst2UI CCWGG 4 cut(s) 1606, 1697, 1728, 1849
Bst4CI ACNGT 3 cut(s) 425, 1094, 1710
Bst6I CTCTTC 5 cut(s) 108, 126, 432, 1056, 2097
BstAFI CTTAAG 1 cut(s) 11
BstAUI TGTACA 1 cut(s) 402
BstDEI CTNAG 8 cut(s) 37, 123, 209, 296, 319, 542, 1356, 1539
BstF5I GGATG 5 cut(s) 760, 795, 952, 1451, 1807
BstH2I RGCGCY 1 cut(s) 1546
BstHHI GCGC 2 cut(s) 1545, 1911
BstMAI GTCTC 2 cut(s) 2057, 2073
BstMWI GCNNNNNNNGC 6 cut(s) 329, 359, 1398, 1622, 1697, 2069
BstNI CCWGG 4 cut(s) 1606, 1697, 1728, 1849
BstNSI RCATGY 3 cut(s) 802, 1396, 2188
BstSCI CCNGG 5 cut(s) 1197, 1604, 1695, 1726, 1847
BstSFI CTRYAG 2 cut(s) 1467, 1577
BstSLI GKGCMC 1 cut(s) 1682
BstV1I GCAGC 6 cut(s) 1634, 1883, 1886, 1913, 2114, 2210
BstV2I GAAGAC 2 cut(s) 134, 2161
BstX2I RGATCY 2 cut(s) 967, 1156
BstXI CCANNNNNNTGG 1 cut(s) 732
BstYI RGATCY 2 cut(s) 967, 1156
Bsu15I ATCGAT 1 cut(s) 2014
Bsu36I CCTNAGG 1 cut(s) 209
BsuI GTATCC 2 cut(s) 954, 1255
BsuRI GGCC 9 cut(s) 137, 332, 342, 349, 366, 414, 591, 1021, 2270
BsuTUI ATCGAT 1 cut(s) 2014
BtgZI GCGATG 2 cut(s) 351, 428
BtrI CACGTC 1 cut(s) 508
BtsCI GGATG 5 cut(s) 760, 795, 952, 1451, 1807
BtsI GCAGTG 1 cut(s) 1464
BtsIMutI CAGTG 4 cut(s) 184, 430, 1464, 2160
CaiI CAGNNNCTG 2 cut(s) 26, 1877
CciI TCATGA 2 cut(s) 628, 2172
CfoI GCGC 2 cut(s) 1545, 1911
Cfr10I RCCGGY 1 cut(s) 362
Cfr13I GGNCC 5 cut(s) 136, 365, 575, 1020, 1195
ClaI ATCGAT 1 cut(s) 2014
CseI GACGC 1 cut(s) 107
CsiI ACCWGGT 1 cut(s) 1847
Csp6I GTAC 5 cut(s) 403, 914, 1706, 1845, 2027
CspCI CAANNNNNGTGG 4 cut(s) 160, 195, 1573, 1608
CviQI GTAC 5 cut(s) 403, 914, 1706, 1845, 2027
DdeI CTNAG 8 cut(s) 37, 123, 209, 296, 319, 542, 1356, 1539
DriI GACNNNNNGTC 1 cut(s) 506
EaeI YGGCCR 3 cut(s) 340, 412, 589
Eam1104I CTCTTC 5 cut(s) 108, 126, 432, 1056, 2097
Eam1105I GACNNNNNGTC 1 cut(s) 506
EarI CTCTTC 5 cut(s) 108, 126, 432, 1056, 2097
Eco130I CCWWGG 3 cut(s) 387, 956, 2265
Eco24I GRGCYC 1 cut(s) 2230
Eco31I GGTCTC 2 cut(s) 2057, 2073
Eco47I GGWCC 2 cut(s) 575, 1195
Eco47III AGCGCT 1 cut(s) 1544
Eco57I CTGAAG 3 cut(s) 132, 456, 1239
Eco81I CCTNAGG 1 cut(s) 209
EcoO109I RGGNCCY 2 cut(s) 575, 1195
EcoRI GAATTC 1 cut(s) 1637
EcoRII CCWGG 4 cut(s) 1604, 1695, 1726, 1847
EcoT14I CCWWGG 3 cut(s) 387, 956, 2265
EcoT22I ATGCAT 1 cut(s) 762
EcoT38I GRGCYC 1 cut(s) 2230
ErhI CCWWGG 3 cut(s) 387, 956, 2265
FaqI GGGAC 5 cut(s) 44, 513, 1181, 1181, 2079
FauI CCCGC 2 cut(s) 2056, 2241
FauNDI CATATG 2 cut(s) 395, 1840
FbaI TGATCA 2 cut(s) 631, 1035
Fnu4HI GCNGC 6 cut(s) 1623, 1872, 1875, 1902, 2103, 2199
FokI GGATG 5 cut(s) 747, 802, 939, 1458, 1814
FriOI GRGCYC 1 cut(s) 2230
Fsp4HI GCNGC 6 cut(s) 1623, 1872, 1875, 1902, 2103, 2199
FspBI CTAG 1 cut(s) 1329
FspI TGCGCA 1 cut(s) 1910
GlaI GCGC 2 cut(s) 1544, 1910
GluI GCNGC 6 cut(s) 1623, 1872, 1875, 1902, 2103, 2199
GsaI CCCAGC 2 cut(s) 30, 2152
GsuI CTGGAG 2 cut(s) 225, 2184
HaeII RGCGCY 1 cut(s) 1546
HaeIII GGCC 9 cut(s) 137, 332, 342, 349, 366, 414, 591, 1021, 2270
HapII CCGG 3 cut(s) 363, 1199, 1799
HgaI GACGC 1 cut(s) 107
HhaI GCGC 2 cut(s) 1545, 1911
Hin6I GCGC 2 cut(s) 1543, 1909
HinP1I GCGC 2 cut(s) 1543, 1909
HindIII AAGCTT 2 cut(s) 8, 600
HinfI GANTC 5 cut(s) 32, 625, 736, 1553, 2166
HpaII CCGG 3 cut(s) 363, 1199, 1799
HphI GGTGA 6 cut(s) 194, 1391, 1535, 1753, 2150, 2273
Hpy166II GTNNAC 5 cut(s) 310, 796, 1422, 1680, 2281
Hpy188I TCNGA 7 cut(s) 71, 480, 569, 1040, 1432, 1459, 1513
Hpy188III TCNNGA 7 cut(s) 95, 110, 629, 705, 1253, 1436, 2173
Hpy8I GTNNAC 5 cut(s) 310, 796, 1422, 1680, 2281
Hpy99I CGWCG 3 cut(s) 101, 512, 1310
HpyCH4III ACNGT 3 cut(s) 425, 1094, 1710
HpyCH4IV ACGT 1 cut(s) 507
HpyF10VI GCNNNNNNNGC 6 cut(s) 329, 359, 1398, 1622, 1697, 2069
HpyF3I CTNAG 8 cut(s) 37, 123, 209, 296, 319, 542, 1356, 1539
HpySE526I ACGT 1 cut(s) 507
HspAI GCGC 2 cut(s) 1543, 1909
KflI GGGWCCC 1 cut(s) 1195
KpnI GGTACC 1 cut(s) 1848
KroI GCCGGC 1 cut(s) 362
KroNI GCCGGC 1 cut(s) 364
Ksp22I TGATCA 2 cut(s) 631, 1035
LguI GCTCTTC 3 cut(s) 108, 432, 1056
Lsp1109I GCAGC 6 cut(s) 1634, 1883, 1886, 1913, 2114, 2210
LweI GCATC 5 cut(s) 451, 769, 1192, 1224, 1494
MabI ACCWGGT 1 cut(s) 1847
MaeI CTAG 1 cut(s) 1329
MaeII ACGT 1 cut(s) 507
MaeIII GTNAC 5 cut(s) 461, 515, 643, 1057, 1379
MbiI CCGCTC 1 cut(s) 2002
MfeI CAATTG 2 cut(s) 860, 901
MflI RGATCY 2 cut(s) 967, 1156
MhlI GDGCHC 2 cut(s) 1682, 2230
MlsI TGGCCA 3 cut(s) 342, 414, 591
MluNI TGGCCA 3 cut(s) 342, 414, 591
MlyI GAGTC 2 cut(s) 26, 2175
MmeI TCCRAC 1 cut(s) 49
Mox20I TGGCCA 3 cut(s) 342, 414, 591
Mph1103I ATGCAT 1 cut(s) 762
MroNI GCCGGC 1 cut(s) 362
MroXI GAANNNNTTC 1 cut(s) 221
MscI TGGCCA 3 cut(s) 342, 414, 591
MseI TTAA 5 cut(s) 12, 420, 1478, 1713, 2118
MslI CAYNNNNRTG 8 cut(s) 398, 480, 554, 633, 639, 803, 924, 1668
Msp20I TGGCCA 3 cut(s) 342, 414, 591
MspA1I CMGCKG 1 cut(s) 1877
MspCI CTTAAG 1 cut(s) 11
MspI CCGG 3 cut(s) 363, 1199, 1799
MspR9I CCNGG 5 cut(s) 1199, 1606, 1697, 1728, 1849
MunI CAATTG 2 cut(s) 860, 901
MvaI CCWGG 4 cut(s) 1606, 1697, 1728, 1849
MwoI GCNNNNNNNGC 6 cut(s) 329, 359, 1398, 1622, 1697, 2069
NaeI GCCGGC 1 cut(s) 364
NciI CCSGG 1 cut(s) 1199
NdeI CATATG 2 cut(s) 395, 1840
NgoMIV GCCGGC 1 cut(s) 362
NlaIV GGNNCC 6 cut(s) 1196, 1197, 1536, 1611, 1846, 2253
NmuCI GTSAC 4 cut(s) 515, 643, 1057, 1379
NsbI TGCGCA 1 cut(s) 1910
NsiI ATGCAT 1 cut(s) 762
NspI RCATGY 3 cut(s) 802, 1396, 2188
PaeI GCATGC 2 cut(s) 1396, 2188
PagI TCATGA 2 cut(s) 628, 2172
PciSI GCTCTTC 3 cut(s) 108, 432, 1056
PdiI GCCGGC 1 cut(s) 364
PdmI GAANNNNTTC 1 cut(s) 221
PfeI GAWTC 3 cut(s) 625, 736, 1553
PfoI TCCNGGA 1 cut(s) 1197
PkrI GCNGC 6 cut(s) 1624, 1873, 1876, 1903, 2104, 2200
PleI GAGTC 2 cut(s) 26, 2174
PpsI GAGTC 2 cut(s) 26, 2174
PpuMI RGGWCCY 2 cut(s) 575, 1195
PshBI ATTAAT 1 cut(s) 2118
PsiI TTATAA 1 cut(s) 2114
Psp5II RGGWCCY 2 cut(s) 575, 1195
Psp6I CCWGG 4 cut(s) 1604, 1695, 1726, 1847
PspFI CCCAGC 2 cut(s) 26, 2148
PspGI CCWGG 4 cut(s) 1604, 1695, 1726, 1847
PspN4I GGNNCC 6 cut(s) 1196, 1197, 1536, 1611, 1846, 2253
PspPI GGNCC 5 cut(s) 136, 365, 575, 1020, 1195
PspPPI RGGWCCY 2 cut(s) 575, 1195
PsrI GAACNNNNNNTAC 2 cut(s) 1082, 1114
PstI CTGCAG 1 cut(s) 1471
PstNI CAGNNNCTG 2 cut(s) 26, 1877
PsuI RGATCY 2 cut(s) 967, 1156
PvuII CAGCTG 1 cut(s) 1877
RsaI GTAC 5 cut(s) 404, 915, 1707, 1846, 2028
RsaNI GTAC 5 cut(s) 403, 914, 1706, 1845, 2027
RseI CAYNNNNRTG 8 cut(s) 398, 480, 554, 633, 639, 803, 924, 1668
SapI GCTCTTC 3 cut(s) 108, 432, 1056
SaqAI TTAA 5 cut(s) 12, 420, 1478, 1713, 2118
SatI GCNGC 6 cut(s) 1623, 1872, 1875, 1902, 2103, 2199
Sau96I GGNCC 5 cut(s) 136, 365, 575, 1020, 1195
ScaI AGTACT 1 cut(s) 1707
SchI GAGTC 2 cut(s) 26, 2175
ScrFI CCNGG 5 cut(s) 1199, 1606, 1697, 1728, 1849
SduI GDGCHC 2 cut(s) 1682, 2230
SexAI ACCWGGT 1 cut(s) 1847
SfaNI GCATC 5 cut(s) 451, 769, 1192, 1224, 1494
SfcI CTRYAG 2 cut(s) 1467, 1577
SinI GGWCC 2 cut(s) 575, 1195
SmiMI CAYNNNNRTG 8 cut(s) 398, 480, 554, 633, 639, 803, 924, 1668
SmlI CTYRAG 1 cut(s) 11
SmoI CTYRAG 1 cut(s) 11
SphI GCATGC 2 cut(s) 1396, 2188
SsiI CCGC 9 cut(s) 160, 353, 1399, 1916, 1982, 2000, 2063, 2242, 2248
SspMI CTAG 1 cut(s) 1329
StyD4I CCNGG 5 cut(s) 1197, 1604, 1695, 1726, 1847
StyI CCWWGG 3 cut(s) 387, 956, 2265
TaaI ACNGT 3 cut(s) 425, 1094, 1710
TaiI ACGT 1 cut(s) 510
TaqI TCGA 6 cut(s) 510, 680, 1096, 1499, 2004, 2014
TatI WGTACW 3 cut(s) 402, 1705, 2026
TfiI GAWTC 3 cut(s) 625, 736, 1553
Tru1I TTAA 5 cut(s) 12, 420, 1478, 1713, 2118
Tru9I TTAA 5 cut(s) 12, 420, 1478, 1713, 2118
TscAI CASTG 4 cut(s) 184, 430, 1471, 2167
TseFI GTSAC 4 cut(s) 515, 643, 1057, 1379
TseI GCWGC 6 cut(s) 1622, 1871, 1874, 1901, 2102, 2198
Tsp45I GTSAC 4 cut(s) 515, 643, 1057, 1379
TspRI CASTG 4 cut(s) 184, 430, 1471, 2167
Vha464I CTTAAG 1 cut(s) 11
VneI GTGCAC 1 cut(s) 1678
VpaK11BI GGWCC 2 cut(s) 575, 1195
VspI ATTAAT 1 cut(s) 2118
XapI RAATTY 2 cut(s) 453, 1637
XceI RCATGY 3 cut(s) 802, 1396, 2188
XcmI CCANNNNNNNNNTGG 2 cut(s) 771, 1114
XmnI GAANNNNTTC 1 cut(s) 221
XspI CTAG 1 cut(s) 1329
ZrmI AGTACT 1 cut(s) 1707
Zsp2I ATGCAT 1 cut(s) 762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.