MD17G1245800.v1.1

Belongs to the terpene cyclase mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000173
Physical Location & Seq
Forward (+)
29422196 .. 29428905
6710 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1245800.v1.1.491

Sequence Viewer

Length: 2295 bp
ATGTGGAAGCTTAAGGTCGCAGATGGTGGCAATGATCCATACATCTACAGCACAAACAACTTCGTGGGTAGGCAGATATTTGAGTTTGACCCTGAAGCAGGAACTACCGAGGAGCGAGCTGAGGTGGAAGAAGCTCGTCTTCATTTCTACAATAATCGCTATCAGGTTAAGCCCAATAGTGACCTCCTATGGCGAATGCAGTTCCTAAGAGAGAAGAACTTCAAACAGACAATTCCCCCAGTGATAGTTGAGGATGGAGAGGAAATCACATACGAAAAGGCCACCGCTGCATTGAGAAGGTCTGTCCACTTCTTTTCAGCCTTGCAAGCTAGTGATGGCCATTGGCCAGCTGAAAATGCCGGCCCGTTGTTTTTCCTTCCTCCTTTGGTCATGTGCACTTACATTACGGGACATCTTACTACTGTATTCCTTGAAGAACACCGCAAAGAAATTTTGCGTTACATATACTATCATCAGAACCAAGATGGTGGTTGGGGATTACACATTGAAGGACACAGCACCATGTTCTGCACAGCTCTCAACTACATTTGTATGCGTATTCTTGGAGAAGGCCCTGATGGTGGCCAAGACAATGCTTGTGCAAGAGCTAGAAAGTGGATTCTTGATCATGGGAGTGTTACCCACATCCCCTCTTGGGGTAAAACTTGGCTTTCGATACTTGGTGTGTTTGAGTGGTCCGGAAGCAACCCAATGCCCCCAGAGTTTTGGATTCTGCCTTCCTTTCTTCCTATGTATCCAGGAAAAATTTGGTGCTTTTGTAGGACTGTTTACAAGCCTATGTCATATTTATATGGCATAAGATTTGTTGGTCCAATAACACCTCTCATACTACAATTAAGGGAAGAACTCTATGCTCAACCTTATGACGAAATTAATTGGAAGGGAGTGCGTCATCACTGCGCTAAAGTGGACATCTACTACCCTCATCCTTGGATACAGGACTTTCTATGGGATAGTCTGTACGTATGTACAGAGCCTCTTCTTACTCGCTGGCCGTTTAACAAGCTGATCAGGAAAAGGGCTCTTGAAGTAACAATGGAGCATGTTCATTATGAAGATGAAAACAGCAGATACATTACCCATGGATGTGTGGAAAAGGTGTTATGCATGCTAGCTTGTTGGGTGGAAGATCCAAATGGGGATTATTTTAAAAAGCATCTTGCCAGGATAACAGATTATTTATGGGTTGCTGAGGATGGGATGAAGATGCAAAGTTTTGGCAGTCAACAGTGGGATACTGGTCTTGCCATTCAAGCTTTGCTTGCTTCCAATCTAACTGATGAAATTGCACCCACGCTCGCTAGAGGACATGACTTCGTCAAGAAATCTCAGGTCAAGGACAATCCGTCGGGCGACTTCAAAAGCATGTACCGCCACATTTCCAAAGGATCGTGGACTTTCTCTGATCAAGACCATGGATGGCAAGTTTCTGATTGCACTGCAGAGGGTTTAAAGTGTTGCCTGCTTTTATCGATGATGCCACCAGAGATAGTTGGTGAAAAAATGGAGCCTGAGCAGTTATACGATGCTGTCAATGTCCTAATTTCCCTACAGAGTAAGAATGGTGGCTTAGCAGCCTGGGAACCAGCAGGAGCAGCAGAATGGTTAGAAATGTTGAATCCCACAGAATTCTTTGAGGACATTGTGATTGAGCATGAACACGTTGAGTGCACTTCATCTGCAATCCAGGCTTTAGTTTTGTTTAAGAAGTTATACCCTGGCCATAGGAAGGAAGAGATTGATCAATTTATTACCGCTGCTGCACGGTACCTTGAAAATAAGAAAAAGAACTCAGTGAAGAAACGTTTGCACAGGTACGGGAATTGGGGAGTTTGCTTCACATATGGTACCTGGTTTGCTCTTGGAGGGCTAACTGCTGCTGGTAAGACCTTCAACAATTGTGCAGCCATGCGCAAAGCTGTTAGTTTTCTACTCGCAATCCAGAAAGAGAATGGTGGTTGGGGAGAGAGCTACCTTTCATGTCCAAAAAAGGAGTACGTTCCTCTTGAAGGAAACCGATCAAATTTAGTACACACAGCTTGGGCTATGATGGGTTTGATTCATGCAGGGCAGGCGGAAAGAGACCCCACACCTCTTCATCGTGCAGCAAAGTTGATAATCAATTCTCAGATGGAAAATGGTGATTTTCCTCAGCAGGAAATCACTGGAGTATTCTTGAAGAACTGCATGCTACATTATCCAGCTTATAGAAATATCTTTCCCATGTGGGCACTTGCAGAATACCGCAGGCGGGTTCCGTTCGTTGATGTCTAA

Protein Analysis

765

Amino Acids

87.91

Weight (kDa)

6.23

Isoelectric Point (pI)

44.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQHop_cyclase_N PF13249 99 - 401 2.4e-32 Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C PF13243 417 - 757 9.7e-45 Squalene-hopene cyclase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66960 AT1G66960 AT1G66960 AT1G66960 AT1G78950 AT1G78950 AT1G78955 AT1G78955 AT1G78955 AT1G78955 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78970 AT1G78970 AT1G78970
fragaria_vesca FvH4_5g29480 FvH4_6g36450 FvH4_6g36450 FvH4_6g36500 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g37620
malus_domestica MD03G1089600.v1.1 MD09G1167700.v1.1 MD09G1168200.v1.1 MD09G1168300.v1.1 MD11G1098600.v1.1 MD17G1158300.v1.1 MD17G1158700.v1.1 MD17G1159500.v1.1 MD17G1160500.v1.1 MD17G1182500.v1.1 MD17G1182700.v1.1 MD17G1245800.v1.1 MD17G1246300.v1.1 MD17G1246600.v1.1
prunus_persica Prupe.3G025700_v2.0.a1 Prupe.3G025700_v2.0.a1 Prupe.3G025800_v2.0.a1 Prupe.3G025900_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026400_v2.0.a1 Prupe.3G026500_v2.0.a1 Prupe.3G026700_v2.0.a1 Prupe.3G026800_v2.0.a1
pyrus_communis pycom03g07120 pycom09g08520 pycom09g08590 pycom09g08610 pycom10g10070 pycom11g08310 pycom16g19880 pycom17g15190 pycom17g15300
rosa_chinensis RchiOBHm_Chr2g0136221 RchiOBHm_Chr2g0148451 RchiOBHm_Chr2g0148501 RchiOBHm_Chr2g0148521 RchiOBHm_Chr2g0148661 RchiOBHm_Chr2g0148701 RchiOBHm_Chr2g0148811 RchiOBHm_Chr2g0148941 RchiOBHm_Chr2g0148981 RchiOBHm_Chr2g0150451 RchiOBHm_Chr2g0150521 RchiOBHm_Chr2g0150611 RchiOBHm_Chr4g0420831 RchiOBHm_Chr5g0049791 RchiOBHm_Chr5g0049801
rosa_laevigata RLG00000007735 RLG00000020351 RLG00000020352 RLG00000020357 RLG00000020358 RLG00000020360 RLG00000029093 RLG00000030686
rosa_multiflora Rmu_co8274513.1_g000001 Rmu_sc0000048.1_g000003 Rmu_sc0000048.1_g000033 Rmu_sc0000048.1_g000039 Rmu_sc0001397.1_g000011 Rmu_sc0001998.1_g000039 Rmu_sc0002070.1_g000055 Rmu_sc0002082.1_g000052 Rmu_sc0003181.1_g000010 Rmu_sc0005104.1_g000005 Rmu_sc0006758.1_g000002 Rmu_sc0008411.1_g000029 Rmu_sc0008411.1_g000030 Rmu_sc0017472.1_g000001 Rmu_sc0017472.1_g000003 Rmu_sc0029876.1_g000001 Rmu_ssc0000480.1_g000024 Rmu_ssc0000480.1_g000036
rosa_roxburghii Rroxscaffold_2G00097070 Rroxscaffold_2G00098540 Rroxscaffold_2G00098560 Rroxscaffold_2G00098570 Rroxscaffold_2G00098650 Rroxscaffold_2G00098710 Rroxscaffold_2G00098740 Rroxscaffold_3G00225830 Rroxscaffold_3G00236870 Rroxscaffold_4G00312240
rosa_rugosa Rorug01G0047000 Rorug02G0372300 Rorug02G0407200 Rorug02G0407300 Rorug02G0408400 Rorug02G0421500 Rorug07G0208900
rosa_samantha Rh2AG380200 Rh2AG465700 Rh2AG465800 Rh2AG466800 Rh2AG467100 Rh2AG467600 Rh2AG480900 Rh2AG481400 Rh2AG481700 Rh2CG073500 Rh2CG452200 Rh2CG453200 Rh2CG453600 Rh2CG454100 Rh2CG467100 Rh2CG467800 Rh2CG468000 Rh2DG403100 Rh2DG487800 Rh2DG487900 Rh2DG488800 Rh2DG502000 Rh2DG502200 Rh2DG502300 Rh2DG503100 Rh3AG319300 Rh4AG227700 Rh4BG371800 Rh5AG327400 Rh5DG350600 Rh7AG349800
rosa_wichuraiana Rw0G010080 Rw2G031110 Rw2G037980 Rw2G037990 Rw2G038000 Rw2G038010 Rw2G038080 Rw2G038100 Rw2G039260 Rw2G039290 Rw2G039470 Rw4G019710 Rw5G030930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1934
Acc65I GGTACC 2 cut(s) 1788, 1868
AccB1I GGYRCC 2 cut(s) 1788, 1868
AccIII TCCGGA 1 cut(s) 698
AciI CCGC 7 cut(s) 285, 442, 1393, 1776, 2096, 2266, 2272
AclI AACGTT 1 cut(s) 1825
AclWI GGATC 3 cut(s) 29, 1145, 1417
AcoI YGGCCR 5 cut(s) 337, 344, 583, 1013, 1741
AcsI RAATTY 4 cut(s) 450, 765, 1649, 2044
AcuI CTGAAG 1 cut(s) 114
AfaI GTAC 8 cut(s) 983, 991, 1391, 1790, 1838, 1870, 2018, 2052
AfiI CCNNNNNNNGG 7 cut(s) 98, 581, 655, 656, 1750, 2030, 2272
AflII CTTAAG 1 cut(s) 11
AflIII ACRYGT 1 cut(s) 1681
AhdI GACNNNNNGTC 1 cut(s) 1366
AjnI CCWGG 6 cut(s) 757, 1184, 1598, 1707, 1738, 1871
Alw21I GWGCWC 2 cut(s) 398, 1694
Alw26I GTCTC 1 cut(s) 2097
Alw44I GTGCAC 2 cut(s) 394, 1690
AlwI GGATC 3 cut(s) 29, 1145, 1417
Aor13HI TCCGGA 1 cut(s) 698
AoxI GGCC 8 cut(s) 279, 337, 344, 361, 571, 583, 1013, 1741
ApaLI GTGCAC 2 cut(s) 394, 1690
ApeKI GCWGC 8 cut(s) 287, 1595, 1616, 1778, 1781, 1898, 1925, 2126
ApoI RAATTY 4 cut(s) 450, 765, 1649, 2044
AseI ATTAAT 1 cut(s) 894
Asp700I GAANNNNTTC 1 cut(s) 218
Asp718I GGTACC 2 cut(s) 1788, 1868
AspLEI GCGC 2 cut(s) 923, 1935
AspS9I GGNCC 4 cut(s) 362, 572, 696, 830
AsuHPI GGTGA 2 cut(s) 1529, 2174
AsuNHI GCTAGC 1 cut(s) 1132
AvaII GGWCC 2 cut(s) 696, 830
BaeGI GKGCMC 3 cut(s) 398, 1694, 2254
BalI TGGCCA 4 cut(s) 339, 346, 585, 1743
BanI GGYRCC 2 cut(s) 1788, 1868
BanII GRGCYC 1 cut(s) 1045
BbsI GAAGAC 1 cut(s) 131
Bbv12I GWGCWC 2 cut(s) 398, 1694
BbvCI CCTCAGC 3 cut(s) 120, 1212, 2172
BbvI GCAGC 8 cut(s) 274, 1607, 1628, 1765, 1768, 1885, 1937, 2138
BccI CCATC 9 cut(s) 17, 248, 329, 479, 572, 1211, 1434, 2065, 2146
BceAI ACGGC 1 cut(s) 1000
BciT130I CCWGG 6 cut(s) 759, 1186, 1600, 1709, 1740, 1873
BciVI GTATCC 3 cut(s) 765, 948, 1249
BclI TGATCA 4 cut(s) 625, 1029, 1426, 1762
BcoDI GTCTC 1 cut(s) 2097
BfaI CTAG 4 cut(s) 330, 609, 1133, 1323
BfmI CTRYAG 3 cut(s) 46, 1461, 1571
BfrI CTTAAG 1 cut(s) 11
BfuI GTATCC 3 cut(s) 765, 948, 1249
BisI GCNGC 8 cut(s) 288, 1596, 1617, 1779, 1782, 1899, 1926, 2127
BlpI GCTNAGC 1 cut(s) 1591
BlsI GCNGC 8 cut(s) 289, 1597, 1618, 1780, 1783, 1900, 1927, 2128
Bme1390I CCNGG 6 cut(s) 759, 1186, 1600, 1709, 1740, 1873
Bme18I GGWCC 2 cut(s) 696, 830
BmeRI GACNNNNNGTC 1 cut(s) 1366
BmgT120I GGNCC 4 cut(s) 362, 572, 696, 830
BmiI GGNNCC 5 cut(s) 1530, 1605, 1790, 1870, 2277
BmrFI CCNGG 6 cut(s) 759, 1186, 1600, 1709, 1740, 1873
BmrI ACTGGG 1 cut(s) 233
BmsI GCATC 4 cut(s) 1186, 1218, 1488, 1537
BmtI GCTAGC 1 cut(s) 1136
BmuI ACTGGG 1 cut(s) 233
BpiI GAAGAC 1 cut(s) 131
BpmI CTGGAG 1 cut(s) 2208
Bpu10I CCTNAGC 4 cut(s) 120, 1212, 1533, 2172
Bpu1102I GCTNAGC 1 cut(s) 1591
Bsa29I ATCGAT 1 cut(s) 1493
BsaAI YACGTR 1 cut(s) 985
BsaI GGTCTC 1 cut(s) 2097
BsaJI CCNNGG 6 cut(s) 108, 950, 1102, 1435, 1599, 1738
BsaWI WCCGGW 1 cut(s) 698
Bsc4I CCNNNNNNNGG 7 cut(s) 98, 581, 655, 656, 1750, 2030, 2272
Bse118I RCCGGY 1 cut(s) 359
Bse1I ACTGG 3 cut(s) 239, 1264, 2191
Bse3DI GCAATG 1 cut(s) 37
BseAI TCCGGA 1 cut(s) 698
BseBI CCWGG 6 cut(s) 759, 1186, 1600, 1709, 1740, 1873
BseCI ATCGAT 1 cut(s) 1493
BseDI CCNNGG 6 cut(s) 108, 950, 1102, 1435, 1599, 1738
BseGI GGATG 7 cut(s) 259, 645, 946, 1112, 1222, 1227, 1445
BseLI CCNNNNNNNGG 7 cut(s) 98, 581, 655, 656, 1750, 2030, 2272
BseMI GCAATG 1 cut(s) 37
BseMII CTCAG 7 cut(s) 111, 1203, 1364, 1524, 1827, 2162, 2186
BseNI ACTGG 3 cut(s) 239, 1264, 2191
BseRI GAGGAG 1 cut(s) 125
BseSI GKGCMC 3 cut(s) 398, 1694, 2254
BseXI GCAGC 8 cut(s) 274, 1607, 1628, 1765, 1768, 1885, 1937, 2138
BsgI GTGCAG 4 cut(s) 514, 1767, 1944, 2145
BshFI GGCC 8 cut(s) 281, 339, 346, 363, 573, 585, 1015, 1743
BshNI GGYRCC 2 cut(s) 1788, 1868
BshVI ATCGAT 1 cut(s) 1493
BsiHKAI GWGCWC 2 cut(s) 398, 1694
BsiSI CCGG 2 cut(s) 360, 699
BslFI GGGAC 1 cut(s) 423
BslI CCNNNNNNNGG 7 cut(s) 98, 581, 655, 656, 1750, 2030, 2272
BsmAI GTCTC 1 cut(s) 2097
BsmFI GGGAC 1 cut(s) 423
BsmI GAATGC 1 cut(s) 201
BsnI GGCC 8 cut(s) 281, 339, 346, 363, 573, 585, 1015, 1743
Bso31I GGTCTC 1 cut(s) 2097
Bsp1286I GDGCHC 4 cut(s) 398, 1045, 1694, 2254
Bsp13I TCCGGA 1 cut(s) 698
Bsp1407I TGTACA 1 cut(s) 989
Bsp143I GATC 8 cut(s) 34, 625, 1029, 1150, 1409, 1426, 1762, 2039
Bsp1720I GCTNAGC 1 cut(s) 1591
Bsp19I CCATGG 2 cut(s) 1102, 1435
BspACI CCGC 7 cut(s) 285, 442, 1393, 1776, 2096, 2266, 2272
BspANI GGCC 8 cut(s) 281, 339, 346, 363, 573, 585, 1015, 1743
BspCNI CTCAG 7 cut(s) 112, 1204, 1363, 1525, 1826, 2161, 2185
BspDI ATCGAT 1 cut(s) 1493
BspEI TCCGGA 1 cut(s) 698
BspLI GGNNCC 5 cut(s) 1530, 1605, 1790, 1870, 2277
BspMAI CTGCAG 1 cut(s) 1465
BspOI GCTAGC 1 cut(s) 1136
BspPI GGATC 3 cut(s) 29, 1145, 1417
BspT107I GGYRCC 2 cut(s) 1788, 1868
BspTI CTTAAG 1 cut(s) 11
BspTNI GGTCTC 1 cut(s) 2097
BsrDI GCAATG 1 cut(s) 37
BsrFI RCCGGY 1 cut(s) 359
BsrGI TGTACA 1 cut(s) 989
BsrI ACTGG 3 cut(s) 239, 1264, 2191
BssAI RCCGGY 1 cut(s) 359
BssECI CCNNGG 6 cut(s) 108, 950, 1102, 1435, 1599, 1738
BssMI GATC 8 cut(s) 34, 625, 1029, 1150, 1409, 1426, 1762, 2039
BssT1I CCWWGG 3 cut(s) 950, 1102, 1435
Bst2UI CCWGG 6 cut(s) 759, 1186, 1600, 1709, 1740, 1873
Bst4CI ACNGT 4 cut(s) 424, 787, 1251, 1788
Bst6I CTCTTC 3 cut(s) 1005, 1749, 2121
BstAFI CTTAAG 1 cut(s) 11
BstAUI TGTACA 1 cut(s) 989
BstBAI YACGTR 1 cut(s) 985
BstDEI CTNAG 9 cut(s) 120, 206, 1212, 1350, 1533, 1591, 1813, 2148, 2172
BstDSI CCRYGG 2 cut(s) 1102, 1435
BstENI CCTNNNNNAGG 2 cut(s) 96, 2028
BstF5I GGATG 7 cut(s) 259, 645, 946, 1112, 1222, 1227, 1445
BstHHI GCGC 2 cut(s) 923, 1935
BstKTI GATC 8 cut(s) 37, 628, 1032, 1153, 1412, 1429, 1765, 2042
BstMAI GTCTC 1 cut(s) 2097
BstMBI GATC 8 cut(s) 34, 625, 1029, 1150, 1409, 1426, 1762, 2039
BstMWI GCNNNNNNNGC 9 cut(s) 287, 326, 356, 1274, 1283, 1392, 1616, 1709, 2093
BstNI CCWGG 6 cut(s) 759, 1186, 1600, 1709, 1740, 1873
BstNSI RCATGY 4 cut(s) 1067, 1132, 1390, 2212
BstSCI CCNGG 6 cut(s) 757, 1184, 1598, 1707, 1738, 1871
BstSFI CTRYAG 3 cut(s) 46, 1461, 1571
BstSLI GKGCMC 3 cut(s) 398, 1694, 2254
BstSNI TACGTA 1 cut(s) 985
BstV1I GCAGC 8 cut(s) 274, 1607, 1628, 1765, 1768, 1885, 1937, 2138
BstV2I GAAGAC 1 cut(s) 131
BstX2I RGATCY 1 cut(s) 1150
BstXI CCANNNNNNTGG 2 cut(s) 488, 726
BstYI RGATCY 1 cut(s) 1150
Bsu15I ATCGAT 1 cut(s) 1493
BsuI GTATCC 3 cut(s) 765, 948, 1249
BsuRI GGCC 8 cut(s) 281, 339, 346, 363, 573, 585, 1015, 1743
BsuTUI ATCGAT 1 cut(s) 1493
BtgI CCRYGG 2 cut(s) 1102, 1435
BtsCI GGATG 7 cut(s) 259, 645, 946, 1112, 1222, 1227, 1445
BtsI GCAGTG 2 cut(s) 916, 1458
BtsIMutI CAGTG 6 cut(s) 246, 916, 1256, 1458, 1821, 2184
CfoI GCGC 2 cut(s) 923, 1935
Cfr10I RCCGGY 1 cut(s) 359
Cfr13I GGNCC 4 cut(s) 362, 572, 696, 830
ClaI ATCGAT 1 cut(s) 1493
CseI GACGC 1 cut(s) 899
CsiI ACCWGGT 1 cut(s) 1871
Csp6I GTAC 8 cut(s) 982, 990, 1390, 1789, 1837, 1869, 2017, 2051
CviQI GTAC 8 cut(s) 982, 990, 1390, 1789, 1837, 1869, 2017, 2051
DdeI CTNAG 9 cut(s) 120, 206, 1212, 1350, 1533, 1591, 1813, 2148, 2172
DpnI GATC 8 cut(s) 36, 627, 1031, 1152, 1411, 1428, 1764, 2041
DpnII GATC 8 cut(s) 34, 625, 1029, 1150, 1409, 1426, 1762, 2039
DraI TTTAAA 2 cut(s) 1171, 1473
DriI GACNNNNNGTC 1 cut(s) 1366
EaeI YGGCCR 5 cut(s) 337, 344, 583, 1013, 1741
Eam1104I CTCTTC 3 cut(s) 1005, 1749, 2121
Eam1105I GACNNNNNGTC 1 cut(s) 1366
EarI CTCTTC 3 cut(s) 1005, 1749, 2121
EciI GGCGGA 1 cut(s) 2111
Eco105I TACGTA 1 cut(s) 985
Eco130I CCWWGG 3 cut(s) 950, 1102, 1435
Eco24I GRGCYC 1 cut(s) 1045
Eco31I GGTCTC 1 cut(s) 2097
Eco47I GGWCC 2 cut(s) 696, 830
Eco57I CTGAAG 1 cut(s) 114
EcoNI CCTNNNNNAGG 2 cut(s) 96, 2028
EcoO109I RGGNCCY 1 cut(s) 572
EcoRI GAATTC 1 cut(s) 1649
EcoRII CCWGG 6 cut(s) 757, 1184, 1598, 1707, 1738, 1871
EcoT14I CCWWGG 3 cut(s) 950, 1102, 1435
EcoT22I ATGCAT 1 cut(s) 1130
EcoT38I GRGCYC 1 cut(s) 1045
ErhI CCWWGG 3 cut(s) 950, 1102, 1435
FalI AAGNNNNNCTT 4 cut(s) 123, 155, 1266, 1298
FaqI GGGAC 1 cut(s) 423
FauI CCCGC 1 cut(s) 2265
FauNDI CATATG 1 cut(s) 1864
FbaI TGATCA 4 cut(s) 625, 1029, 1426, 1762
Fnu4HI GCNGC 8 cut(s) 288, 1596, 1617, 1779, 1782, 1899, 1926, 2127
FokI GGATG 7 cut(s) 266, 632, 933, 1119, 1229, 1234, 1452
FriOI GRGCYC 1 cut(s) 1045
Fsp4HI GCNGC 8 cut(s) 288, 1596, 1617, 1779, 1782, 1899, 1926, 2127
FspBI CTAG 4 cut(s) 330, 609, 1133, 1323
FspI TGCGCA 1 cut(s) 1934
GlaI GCGC 2 cut(s) 922, 1934
GluI GCNGC 8 cut(s) 288, 1596, 1617, 1779, 1782, 1899, 1926, 2127
GsuI CTGGAG 1 cut(s) 2208
HaeIII GGCC 8 cut(s) 281, 339, 346, 363, 573, 585, 1015, 1743
HapII CCGG 2 cut(s) 360, 699
HgaI GACGC 1 cut(s) 899
HhaI GCGC 2 cut(s) 923, 1935
Hin6I GCGC 2 cut(s) 921, 1933
HinP1I GCGC 2 cut(s) 921, 1933
HincII GTYRAC 1 cut(s) 1247
HindII GTYRAC 1 cut(s) 1247
HindIII AAGCTT 2 cut(s) 8, 1275
HinfI GANTC 4 cut(s) 619, 730, 1639, 2080
HpaII CCGG 2 cut(s) 360, 699
HphI GGTGA 2 cut(s) 1529, 2174
Hpy166II GTNNAC 8 cut(s) 307, 396, 790, 931, 1247, 1416, 1692, 2053
Hpy188I TCNGA 4 cut(s) 477, 1426, 1453, 2151
Hpy188III TCNNGA 9 cut(s) 623, 699, 1033, 1046, 1342, 1430, 1963, 2027, 2197
Hpy8I GTNNAC 8 cut(s) 307, 396, 790, 931, 1247, 1416, 1692, 2053
Hpy99I CGWCG 1 cut(s) 1372
HpyAV CCTTC 9 cut(s) 291, 386, 503, 563, 747, 895, 1744, 1921, 2024
HpyCH4III ACNGT 4 cut(s) 424, 787, 1251, 1788
HpyCH4IV ACGT 4 cut(s) 984, 1683, 1825, 2019
HpyF10VI GCNNNNNNNGC 9 cut(s) 287, 326, 356, 1274, 1283, 1392, 1616, 1709, 2093
HpyF3I CTNAG 9 cut(s) 120, 206, 1212, 1350, 1533, 1591, 1813, 2148, 2172
HpySE526I ACGT 4 cut(s) 984, 1683, 1825, 2019
HspAI GCGC 2 cut(s) 921, 1933
Kpn2I TCCGGA 1 cut(s) 698
KpnI GGTACC 2 cut(s) 1792, 1872
KroI GCCGGC 1 cut(s) 359
KroNI GCCGGC 1 cut(s) 361
Ksp22I TGATCA 4 cut(s) 625, 1029, 1426, 1762
Kzo9I GATC 8 cut(s) 34, 625, 1029, 1150, 1409, 1426, 1762, 2039
LmnI GCTCC 4 cut(s) 112, 1060, 1528, 1613
Lsp1109I GCAGC 8 cut(s) 274, 1607, 1628, 1765, 1768, 1885, 1937, 2138
LweI GCATC 4 cut(s) 1186, 1218, 1488, 1537
MabI ACCWGGT 1 cut(s) 1871
MaeI CTAG 4 cut(s) 330, 609, 1133, 1323
MaeII ACGT 4 cut(s) 984, 1683, 1825, 2019
MaeIII GTNAC 4 cut(s) 179, 458, 637, 1051
MalI GATC 8 cut(s) 36, 627, 1031, 1152, 1411, 1428, 1764, 2041
MboI GATC 8 cut(s) 34, 625, 1029, 1150, 1409, 1426, 1762, 2039
MfeI CAATTG 1 cut(s) 1918
MflI RGATCY 1 cut(s) 1150
MhlI GDGCHC 4 cut(s) 398, 1045, 1694, 2254
MlsI TGGCCA 4 cut(s) 339, 346, 585, 1743
MluNI TGGCCA 4 cut(s) 339, 346, 585, 1743
Mox20I TGGCCA 4 cut(s) 339, 346, 585, 1743
Mph1103I ATGCAT 1 cut(s) 1130
MroI TCCGGA 1 cut(s) 698
MroNI GCCGGC 1 cut(s) 359
MroXI GAANNNNTTC 1 cut(s) 218
MscI TGGCCA 4 cut(s) 339, 346, 585, 1743
MseI TTAA 8 cut(s) 12, 168, 857, 894, 1020, 1170, 1472, 1725
MslI CAYNNNNRTG 3 cut(s) 551, 633, 1107
Msp20I TGGCCA 4 cut(s) 339, 346, 585, 1743
MspA1I CMGCKG 3 cut(s) 287, 350, 1778
MspCI CTTAAG 1 cut(s) 11
MspI CCGG 2 cut(s) 360, 699
MspR9I CCNGG 6 cut(s) 759, 1186, 1600, 1709, 1740, 1873
MunI CAATTG 1 cut(s) 1918
Mva1269I GAATGC 1 cut(s) 201
MvaI CCWGG 6 cut(s) 759, 1186, 1600, 1709, 1740, 1873
MwoI GCNNNNNNNGC 9 cut(s) 287, 326, 356, 1274, 1283, 1392, 1616, 1709, 2093
NaeI GCCGGC 1 cut(s) 361
NcoI CCATGG 2 cut(s) 1102, 1435
NdeI CATATG 1 cut(s) 1864
NdeII GATC 8 cut(s) 34, 625, 1029, 1150, 1409, 1426, 1762, 2039
NgoMIV GCCGGC 1 cut(s) 359
NheI GCTAGC 1 cut(s) 1132
NlaIV GGNNCC 5 cut(s) 1530, 1605, 1790, 1870, 2277
NmuCI GTSAC 1 cut(s) 179
NsbI TGCGCA 1 cut(s) 1934
NsiI ATGCAT 1 cut(s) 1130
NspI RCATGY 4 cut(s) 1067, 1132, 1390, 2212
PaeI GCATGC 2 cut(s) 1132, 2212
PctI GAATGC 1 cut(s) 201
PdiI GCCGGC 1 cut(s) 361
PdmI GAANNNNTTC 1 cut(s) 218
PfeI GAWTC 4 cut(s) 619, 730, 1639, 2080
PflFI GACNNNGTC 1 cut(s) 1337
PfoI TCCNGGA 1 cut(s) 757
PkrI GCNGC 8 cut(s) 289, 1597, 1618, 1780, 1783, 1900, 1927, 2128
Ppu21I YACGTR 1 cut(s) 985
PshBI ATTAAT 1 cut(s) 894
Psp1406I AACGTT 1 cut(s) 1825
Psp6I CCWGG 6 cut(s) 757, 1184, 1598, 1707, 1738, 1871
PspGI CCWGG 6 cut(s) 757, 1184, 1598, 1707, 1738, 1871
PspN4I GGNNCC 5 cut(s) 1530, 1605, 1790, 1870, 2277
PspPI GGNCC 4 cut(s) 362, 572, 696, 830
PstI CTGCAG 1 cut(s) 1465
PsuI RGATCY 1 cut(s) 1150
PsyI GACNNNGTC 1 cut(s) 1337
PvuII CAGCTG 1 cut(s) 350
RsaI GTAC 8 cut(s) 983, 991, 1391, 1790, 1838, 1870, 2018, 2052
RsaNI GTAC 8 cut(s) 982, 990, 1390, 1789, 1837, 1869, 2017, 2051
RseI CAYNNNNRTG 3 cut(s) 551, 633, 1107
SaqAI TTAA 8 cut(s) 12, 168, 857, 894, 1020, 1170, 1472, 1725
SatI GCNGC 8 cut(s) 288, 1596, 1617, 1779, 1782, 1899, 1926, 2127
Sau3AI GATC 8 cut(s) 34, 625, 1029, 1150, 1409, 1426, 1762, 2039
Sau96I GGNCC 4 cut(s) 362, 572, 696, 830
ScrFI CCNGG 6 cut(s) 759, 1186, 1600, 1709, 1740, 1873
SduI GDGCHC 4 cut(s) 398, 1045, 1694, 2254
SexAI ACCWGGT 1 cut(s) 1871
SfaNI GCATC 4 cut(s) 1186, 1218, 1488, 1537
SfcI CTRYAG 3 cut(s) 46, 1461, 1571
SinI GGWCC 2 cut(s) 696, 830
SmiMI CAYNNNNRTG 3 cut(s) 551, 633, 1107
SmlI CTYRAG 1 cut(s) 11
SmoI CTYRAG 1 cut(s) 11
SnaBI TACGTA 1 cut(s) 985
SphI GCATGC 2 cut(s) 1132, 2212
SsiI CCGC 7 cut(s) 285, 442, 1393, 1776, 2096, 2266, 2272
SspMI CTAG 4 cut(s) 330, 609, 1133, 1323
StyD4I CCNGG 6 cut(s) 757, 1184, 1598, 1707, 1738, 1871
StyI CCWWGG 3 cut(s) 950, 1102, 1435
TaaI ACNGT 4 cut(s) 424, 787, 1251, 1788
TaiI ACGT 4 cut(s) 987, 1686, 1828, 2022
TaqI TCGA 2 cut(s) 674, 1493
TatI WGTACW 2 cut(s) 989, 2050
TfiI GAWTC 4 cut(s) 619, 730, 1639, 2080
Tru1I TTAA 8 cut(s) 12, 168, 857, 894, 1020, 1170, 1472, 1725
Tru9I TTAA 8 cut(s) 12, 168, 857, 894, 1020, 1170, 1472, 1725
TscAI CASTG 6 cut(s) 246, 923, 1256, 1465, 1821, 2191
TseFI GTSAC 1 cut(s) 179
TseI GCWGC 8 cut(s) 287, 1595, 1616, 1778, 1781, 1898, 1925, 2126
Tsp45I GTSAC 1 cut(s) 179
TspGWI ACGGA 2 cut(s) 1356, 2268
TspRI CASTG 6 cut(s) 246, 923, 1256, 1465, 1821, 2191
Tth111I GACNNNGTC 1 cut(s) 1337
Vha464I CTTAAG 1 cut(s) 11
VneI GTGCAC 2 cut(s) 394, 1690
VpaK11BI GGWCC 2 cut(s) 696, 830
VspI ATTAAT 1 cut(s) 894
XagI CCTNNNNNAGG 2 cut(s) 96, 2028
XapI RAATTY 4 cut(s) 450, 765, 1649, 2044
XceI RCATGY 4 cut(s) 1067, 1132, 1390, 2212
XcmI CCANNNNNNNNNTGG 2 cut(s) 765, 1970
XmnI GAANNNNTTC 1 cut(s) 218
XspI CTAG 4 cut(s) 330, 609, 1133, 1323
Zsp2I ATGCAT 1 cut(s) 1130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.