RchiOBHm_Chr2g0148811

Belongs to the terpene cyclase mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000173
Physical Location & Seq
Forward (+)
66617868 .. 66622812
4945 bp
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UTR
Exon/CDS
Intron
PRQ51831

Sequence Viewer

Length: 2382 bp
ATGTGGAAGCTTAAGGTTGCAGAAGGTGGGGATTCCTCGGCTTACATTTACAGCACAAACAACTTTGTCGGAAGGCAGACATGGGAGTTTGATTCGGATGCAGGAACTCCTGAAGAGCGAGCTGAGGTTGAAGAGGCCCGTCTTCATTTCTACAACAACCGCTATCAGGTCAAGCCCAGTGGTGACATTCTTTGGCGTATGCAGTTCCTAAGGGAGAAGAAGTTCAAACAGACAATTACTCCAGTAAAAATTGAAGATGGCGAGGTTATCACACATGAAAAAACCACAGATGCACTGAGAAGGTCTGTCCACTTCTTTTCAGCGTTGCAGGCAAGCGATGGCCATTGGCCTGCGGAAAATGCCGGCCCTTTGTTTTTCCTTCCTCCCTTGGTCATATGTATGTACATTACTGGCCATCTTAACAGTGTATTCCCTGAAGAGCATCGCAAAGAAATTCTGCGTTACATATACTATCATCAGAATGAAGATGGTGGTTGGGGACTACACGTCGAAGGTCACAGCACCATGTTCAGCACAGCTCTCAGCTACATTTGTATGCGTATTCTCGGAGAAGGACCTGATGATGGTGGCCAAGACAATGCTTGTCCAAGAGCAAGAAAGTGGATTCTTGATCACGGTGGTGTCACACACATACCCTCTTGGGGAAAGACTTGGCTTTCGATACTTGGTCTGTTTGATTGGTCTGGAAGCAACCCTATGCCCCCAGAGTTTTGGATTCTTCCTTCGTTTCTTCCTATGCATCCAGCAAAAATGTGGTGCTATTGTAGGATGGTTTACATGCCAATGTCATACCTATATGGAAAAAGGTTTGTTGGCCCAATTACACCTCTCATTCTTAAATTGAGGGAGGAACTTTTTATTCAACCTTACAATCAAATCAATTGGAAGAAAGTACGCCATCTATGTGCCAAAGAGGACATCTACTATCCTCATCCTTGGATACAGGATCTCCTATGGGATAGCCTCTACATTTCCACAGAGCCTATTCTTACTCGTTGGCCCTTCAACAAATTGATCAGAGAAAAGGCTCTTCAAGTGACGATGAAGCATATTCATTACGAAGATGAGAACAGTCGATACATTACCATTGGGTGTGTGGAAAAGGTGTTATGTATGCTTGCCTGTTGGGTTGAAGATCCAAATGGGGACTATTTCAAGAAGCATCTGGCAAGGGTCCCGGATTATTTATGGGTTGCTGAAGATGGAATGAAGATGCAAAGTTTTGGCAGTCAGGAGTGGGATACTGGTTTTGCCATTCAAGCTTTGCTTGCTACTAATCTAACGGACGAAATTGGAACAACGCTCGCTAGAGGACATGACTTCATAAAGAAATCTCAGGTCAAAGACAACCCATCTGGTGACTTCAATAGCATGCACCGCCACATTTCCAAAGGATCGTGGACTTTCTCCGATCAAGATCATGGATGGCAAGTTTCTGATTGCACTGCAGAAGGTTTAAAGTGTTGCCTTCTATTGTCGATGATGTCATCGGAGATAGTTGGTGAAAAAATGGAACCTGAGCGCTTGTACGATTCTGTCAATGTTCTACTTTCCCTACAGAGCAAGAATGGTGGTTTGTCAGCCTGGGAACCAGCAGGGGCAGCAGAGTGGTTAGAACTATTAAATCCGACAGAGTTCTTCGCAGACATAGTAATTGAGCATGAATATGTTGAGTGCACTTCATCTGCAATCCAGGCTTTAGTACTGTTTAAGAAGCTACACCCAGGACACCGGAAGAAAGAGATTGAACATTTCATCACCAATGCTATACACTACCTTGAAAATATACAAATGCCGGATGGTTCATGGTATGTATCTATTCTAGCAAATCAATCTATGATTCGAAAACGTGTTAGTTTCACATATACAGAAAAAAAGAACTTGATAAGTAAGCATTTGAACAGGTATGGAAACTGGGGAGTTTGCTTCACATATGGTACCTGGTTTGCAGTAGGAGGATTGGCAGCAGCTGGCAAGACTTTCAGCACTTGTGCAGCCATGCGCAAAGCGGTTAGTTTTCTCCTCACAACGCAGAGAGAGAATGGTGGTTGGGGAGAGAGCTATCTTTCATGTCCGCAAGAGACATACATTCCTCTCGAAGGAAATCGATCCAATTTAGTACATACTGCTTGGGCTATGATGGGTCTCATTCATGCGGGACAGGCAGAAAGAGACCCAACACCTCTTCATCGTGCAGCAAAGTTTATAATTAATTCTCAAATGGAAAATGGTGATTTTCCCCAGCAGGAAATCACTGGAGTCTTCATGAAGAACTGCATGCTACATTATGCAGCTTATAGAAATATATACCCACTCTGGGCTCTTGCAGAATACCGCAAGCGGGTTCCATTGCCTTCCTAG

Protein Analysis

793

Amino Acids

91.17

Weight (kDa)

6.4

Isoelectric Point (pI)

48.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQHop_cyclase_N PF13249 100 - 360 2.9e-35 Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C PF13243 419 - 611 1.8e-13 Squalene-hopene cyclase C-terminal domain
SQHop_cyclase_C PF13243 640 - 787 8.6e-30 Squalene-hopene cyclase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66960 AT1G66960 AT1G66960 AT1G66960 AT1G78950 AT1G78950 AT1G78955 AT1G78955 AT1G78955 AT1G78955 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78970 AT1G78970 AT1G78970
fragaria_vesca FvH4_5g29480 FvH4_6g36450 FvH4_6g36450 FvH4_6g36500 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g37620
malus_domestica MD03G1089600.v1.1 MD09G1167700.v1.1 MD09G1168200.v1.1 MD09G1168300.v1.1 MD11G1098600.v1.1 MD17G1158300.v1.1 MD17G1158700.v1.1 MD17G1159500.v1.1 MD17G1160500.v1.1 MD17G1182500.v1.1 MD17G1182700.v1.1 MD17G1245800.v1.1 MD17G1246300.v1.1 MD17G1246600.v1.1
prunus_persica Prupe.3G025700_v2.0.a1 Prupe.3G025700_v2.0.a1 Prupe.3G025800_v2.0.a1 Prupe.3G025900_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026400_v2.0.a1 Prupe.3G026500_v2.0.a1 Prupe.3G026700_v2.0.a1 Prupe.3G026800_v2.0.a1
pyrus_communis pycom03g07120 pycom09g08520 pycom09g08590 pycom09g08610 pycom10g10070 pycom11g08310 pycom16g19880 pycom17g15190 pycom17g15300
rosa_chinensis RchiOBHm_Chr2g0136221 RchiOBHm_Chr2g0148451 RchiOBHm_Chr2g0148501 RchiOBHm_Chr2g0148521 RchiOBHm_Chr2g0148661 RchiOBHm_Chr2g0148701 RchiOBHm_Chr2g0148811 RchiOBHm_Chr2g0148941 RchiOBHm_Chr2g0148981 RchiOBHm_Chr2g0150451 RchiOBHm_Chr2g0150521 RchiOBHm_Chr2g0150611 RchiOBHm_Chr4g0420831 RchiOBHm_Chr5g0049791 RchiOBHm_Chr5g0049801
rosa_laevigata RLG00000007735 RLG00000020351 RLG00000020352 RLG00000020357 RLG00000020358 RLG00000020360 RLG00000029093 RLG00000030686
rosa_multiflora Rmu_co8274513.1_g000001 Rmu_sc0000048.1_g000003 Rmu_sc0000048.1_g000033 Rmu_sc0000048.1_g000039 Rmu_sc0001397.1_g000011 Rmu_sc0001998.1_g000039 Rmu_sc0002070.1_g000055 Rmu_sc0002082.1_g000052 Rmu_sc0003181.1_g000010 Rmu_sc0005104.1_g000005 Rmu_sc0006758.1_g000002 Rmu_sc0008411.1_g000029 Rmu_sc0008411.1_g000030 Rmu_sc0017472.1_g000001 Rmu_sc0017472.1_g000003 Rmu_sc0029876.1_g000001 Rmu_ssc0000480.1_g000024 Rmu_ssc0000480.1_g000036
rosa_roxburghii Rroxscaffold_2G00097070 Rroxscaffold_2G00098540 Rroxscaffold_2G00098560 Rroxscaffold_2G00098570 Rroxscaffold_2G00098650 Rroxscaffold_2G00098710 Rroxscaffold_2G00098740 Rroxscaffold_3G00225830 Rroxscaffold_3G00236870 Rroxscaffold_4G00312240
rosa_rugosa Rorug01G0047000 Rorug02G0372300 Rorug02G0407200 Rorug02G0407300 Rorug02G0408400 Rorug02G0421500 Rorug07G0208900
rosa_samantha Rh2AG380200 Rh2AG465700 Rh2AG465800 Rh2AG466800 Rh2AG467100 Rh2AG467600 Rh2AG480900 Rh2AG481400 Rh2AG481700 Rh2CG073500 Rh2CG452200 Rh2CG453200 Rh2CG453600 Rh2CG454100 Rh2CG467100 Rh2CG467800 Rh2CG468000 Rh2DG403100 Rh2DG487800 Rh2DG487900 Rh2DG488800 Rh2DG502000 Rh2DG502200 Rh2DG502300 Rh2DG503100 Rh3AG319300 Rh4AG227700 Rh4BG371800 Rh5AG327400 Rh5DG350600 Rh7AG349800
rosa_wichuraiana Rw0G010080 Rw2G031110 Rw2G037980 Rw2G037990 Rw2G038000 Rw2G038010 Rw2G038080 Rw2G038100 Rw2G039260 Rw2G039290 Rw2G039470 Rw4G019710 Rw5G030930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2228
Acc16I TGCGCA 1 cut(s) 2024
Acc65I GGTACC 1 cut(s) 1958
AccB1I GGYRCC 1 cut(s) 1958
AciI CCGC 8 cut(s) 160, 353, 1399, 2030, 2096, 2177, 2356, 2362
AclWI GGATC 4 cut(s) 975, 1151, 1423, 2124
AcoI YGGCCR 3 cut(s) 340, 412, 589
AcsI RAATTY 1 cut(s) 453
AcuI CTGAAG 3 cut(s) 132, 456, 1239
AfaI GTAC 6 cut(s) 404, 915, 1550, 1725, 1960, 2142
AfeI AGCGCT 1 cut(s) 1544
AfiI CCNNNNNNNGG 6 cut(s) 166, 584, 662, 2120, 2338, 2362
AflII CTTAAG 1 cut(s) 11
AflIII ACRYGT 2 cut(s) 505, 1870
AhdI GACNNNNNGTC 1 cut(s) 506
AjiI CACGTC 1 cut(s) 508
AjnI CCWGG 4 cut(s) 1604, 1713, 1744, 1961
AleI CACNNNNGTG 1 cut(s) 639
AloI GAACNNNNNNTCC 2 cut(s) 206, 238
AluBI AGCT 9 cut(s) 10, 122, 539, 546, 1283, 1738, 1991, 2082, 2315
AluI AGCT 9 cut(s) 10, 122, 539, 546, 1283, 1738, 1991, 2082, 2315
Alw21I GWGCWC 1 cut(s) 1700
Alw26I GTCTC 3 cut(s) 2096, 2171, 2187
Alw44I GTGCAC 1 cut(s) 1696
AlwI GGATC 4 cut(s) 975, 1151, 1423, 2124
AlwNI CAGNNNCTG 1 cut(s) 1991
Aor51HI AGCGCT 1 cut(s) 1544
AoxI GGCC 8 cut(s) 135, 340, 347, 364, 412, 589, 835, 1019
ApaLI GTGCAC 1 cut(s) 1696
ApeKI GCWGC 6 cut(s) 1622, 1985, 1988, 2015, 2216, 2312
ApoI RAATTY 1 cut(s) 453
ArsI GACNNNNNNTTYG 2 cut(s) 661, 693
AseI ATTAAT 1 cut(s) 2232
Asp700I GAANNNNTTC 1 cut(s) 221
Asp718I GGTACC 1 cut(s) 1958
AspLEI GCGC 2 cut(s) 1545, 2025
AspS9I GGNCC 6 cut(s) 136, 365, 575, 836, 1020, 1195
AsuC2I CCSGG 1 cut(s) 1199
AsuHPI GGTGA 5 cut(s) 194, 1391, 1535, 1771, 2264
AsuII TTCGAA 1 cut(s) 1864
AvaII GGWCC 2 cut(s) 575, 1195
AxyI CCTNAGG 1 cut(s) 209
BaeGI GKGCMC 1 cut(s) 1700
BalI TGGCCA 3 cut(s) 342, 414, 591
BanI GGYRCC 1 cut(s) 1958
BanII GRGCYC 1 cut(s) 2344
BbsI GAAGAC 2 cut(s) 134, 2275
Bbv12I GWGCWC 1 cut(s) 1700
BbvCI CCTCAGC 1 cut(s) 123
BbvI GCAGC 6 cut(s) 1634, 1997, 2000, 2027, 2228, 2324
BciT130I CCWGG 4 cut(s) 1606, 1715, 1746, 1963
BciVI GTATCC 2 cut(s) 954, 1255
BclI TGATCA 2 cut(s) 631, 1035
BcnI CCSGG 1 cut(s) 1199
BcoDI GTCTC 3 cut(s) 2096, 2171, 2187
BfaI CTAG 3 cut(s) 1329, 1844, 2380
BfmI CTRYAG 2 cut(s) 1467, 1577
BfoI RGCGCY 1 cut(s) 1546
BfrI CTTAAG 1 cut(s) 11
BfuI GTATCC 2 cut(s) 954, 1255
BisI GCNGC 6 cut(s) 1623, 1986, 1989, 2016, 2217, 2313
BlsI GCNGC 6 cut(s) 1624, 1987, 1990, 2017, 2218, 2314
BmcAI AGTACT 1 cut(s) 1725
Bme1390I CCNGG 5 cut(s) 1199, 1606, 1715, 1746, 1963
Bme18I GGWCC 2 cut(s) 575, 1195
BmeRI GACNNNNNGTC 1 cut(s) 506
BmgBI CACGTC 1 cut(s) 508
BmgT120I GGNCC 6 cut(s) 136, 365, 575, 836, 1020, 1195
BmiI GGNNCC 6 cut(s) 1196, 1197, 1536, 1611, 1960, 2367
BmrFI CCNGG 5 cut(s) 1199, 1606, 1715, 1746, 1963
BmrI ACTGGG 2 cut(s) 171, 1945
BmsI GCATC 6 cut(s) 88, 280, 451, 769, 1192, 1224
BmuI ACTGGG 2 cut(s) 171, 1945
BpiI GAAGAC 2 cut(s) 134, 2275
BpmI CTGGAG 2 cut(s) 225, 2298
Bpu10I CCTNAGC 2 cut(s) 123, 1539
Bpu14I TTCGAA 1 cut(s) 1864
BpuMI CCSGG 1 cut(s) 1199
Bsa29I ATCGAT 1 cut(s) 2128
BsaBI GATNNNNATC 1 cut(s) 1437
BsaI GGTCTC 2 cut(s) 2171, 2187
BsaJI CCNNGG 5 cut(s) 36, 387, 956, 1605, 1744
BsaWI WCCGGW 1 cut(s) 1752
BsaXI ACNNNNNCTCC 6 cut(s) 206, 223, 236, 253, 954, 984
Bsc4I CCNNNNNNNGG 6 cut(s) 166, 584, 662, 2120, 2338, 2362
Bse118I RCCGGY 1 cut(s) 362
Bse1I ACTGG 6 cut(s) 177, 242, 415, 1270, 1940, 2281
Bse21I CCTNAGG 1 cut(s) 209
Bse3DI GCAATG 1 cut(s) 2369
Bse8I GATNNNNATC 1 cut(s) 1437
BseBI CCWGG 4 cut(s) 1606, 1715, 1746, 1963
BseCI ATCGAT 1 cut(s) 2128
BseDI CCNNGG 5 cut(s) 36, 387, 956, 1605, 1744
BseGI GGATG 6 cut(s) 103, 760, 795, 952, 1451, 1825
BseJI GATNNNNATC 1 cut(s) 1437
BseLI CCNNNNNNNGG 6 cut(s) 166, 584, 662, 2120, 2338, 2362
BseMI GCAATG 1 cut(s) 2369
BseMII CTCAG 5 cut(s) 114, 287, 556, 1370, 1530
BseNI ACTGG 6 cut(s) 177, 242, 415, 1270, 1940, 2281
BseRI GAGGAG 1 cut(s) 2033
BseSI GKGCMC 1 cut(s) 1700
BseXI GCAGC 6 cut(s) 1634, 1997, 2000, 2027, 2228, 2324
BseYI CCCAGC 1 cut(s) 2262
BsgI GTGCAG 2 cut(s) 2034, 2235
BshFI GGCC 8 cut(s) 137, 342, 349, 366, 414, 591, 837, 1021
BshNI GGYRCC 1 cut(s) 1958
BshVI ATCGAT 1 cut(s) 2128
BsiHKAI GWGCWC 1 cut(s) 1700
BsiSI CCGG 4 cut(s) 363, 1199, 1753, 1817
BslFI GGGAC 4 cut(s) 513, 1181, 1181, 2193
BslI CCNNNNNNNGG 6 cut(s) 166, 584, 662, 2120, 2338, 2362
BsmAI GTCTC 3 cut(s) 2096, 2171, 2187
BsmFI GGGAC 4 cut(s) 513, 1181, 1181, 2193
BsnI GGCC 8 cut(s) 137, 342, 349, 366, 414, 591, 837, 1021
Bso31I GGTCTC 2 cut(s) 2171, 2187
Bsp119I TTCGAA 1 cut(s) 1864
Bsp1286I GDGCHC 2 cut(s) 1700, 2344
Bsp1407I TGTACA 1 cut(s) 402
Bsp143I GATC 8 cut(s) 631, 967, 1035, 1156, 1415, 1432, 1438, 2129
BspACI CCGC 8 cut(s) 160, 353, 1399, 2030, 2096, 2177, 2356, 2362
BspANI GGCC 8 cut(s) 137, 342, 349, 366, 414, 591, 837, 1021
BspCNI CTCAG 5 cut(s) 115, 288, 555, 1369, 1531
BspDI ATCGAT 1 cut(s) 2128
BspHI TCATGA 1 cut(s) 2286
BspLI GGNNCC 6 cut(s) 1196, 1197, 1536, 1611, 1960, 2367
BspMAI CTGCAG 1 cut(s) 1471
BspPI GGATC 4 cut(s) 975, 1151, 1423, 2124
BspQI GCTCTTC 3 cut(s) 108, 432, 1056
BspT104I TTCGAA 1 cut(s) 1864
BspT107I GGYRCC 1 cut(s) 1958
BspTI CTTAAG 1 cut(s) 11
BspTNI GGTCTC 2 cut(s) 2171, 2187
BsrDI GCAATG 1 cut(s) 2369
BsrFI RCCGGY 1 cut(s) 362
BsrGI TGTACA 1 cut(s) 402
BsrI ACTGG 6 cut(s) 177, 242, 415, 1270, 1940, 2281
BssAI RCCGGY 1 cut(s) 362
BssECI CCNNGG 5 cut(s) 36, 387, 956, 1605, 1744
BssMI GATC 8 cut(s) 631, 967, 1035, 1156, 1415, 1432, 1438, 2129
BssT1I CCWWGG 2 cut(s) 387, 956
Bst2UI CCWGG 4 cut(s) 1606, 1715, 1746, 1963
Bst4CI ACNGT 4 cut(s) 425, 638, 1094, 1728
Bst6I CTCTTC 5 cut(s) 108, 126, 432, 1056, 2211
BstAFI CTTAAG 1 cut(s) 11
BstAUI TGTACA 1 cut(s) 402
BstBI TTCGAA 1 cut(s) 1864
BstDEI CTNAG 6 cut(s) 123, 209, 296, 542, 1356, 1539
BstENI CCTNNNNNAGG 1 cut(s) 2118
BstF5I GGATG 6 cut(s) 103, 760, 795, 952, 1451, 1825
BstH2I RGCGCY 1 cut(s) 1546
BstHHI GCGC 2 cut(s) 1545, 2025
BstKTI GATC 8 cut(s) 634, 970, 1038, 1159, 1418, 1435, 1441, 2132
BstMAI GTCTC 3 cut(s) 2096, 2171, 2187
BstMBI GATC 8 cut(s) 631, 967, 1035, 1156, 1415, 1432, 1438, 2129
BstMWI GCNNNNNNNGC 8 cut(s) 329, 359, 1280, 1289, 1398, 1622, 1715, 2183
BstNI CCWGG 4 cut(s) 1606, 1715, 1746, 1963
BstNSI RCATGY 3 cut(s) 802, 1396, 2302
BstSCI CCNGG 5 cut(s) 1197, 1604, 1713, 1744, 1961
BstSFI CTRYAG 2 cut(s) 1467, 1577
BstSLI GKGCMC 1 cut(s) 1700
BstV1I GCAGC 6 cut(s) 1634, 1997, 2000, 2027, 2228, 2324
BstV2I GAAGAC 2 cut(s) 134, 2275
BstX2I RGATCY 2 cut(s) 967, 1156
BstXI CCANNNNNNTGG 1 cut(s) 732
BstYI RGATCY 2 cut(s) 967, 1156
Bsu15I ATCGAT 1 cut(s) 2128
Bsu36I CCTNAGG 1 cut(s) 209
BsuI GTATCC 2 cut(s) 954, 1255
BsuRI GGCC 8 cut(s) 137, 342, 349, 366, 414, 591, 837, 1021
BsuTUI ATCGAT 1 cut(s) 2128
BtgZI GCGATG 2 cut(s) 351, 428
BtrI CACGTC 1 cut(s) 508
BtsCI GGATG 6 cut(s) 103, 760, 795, 952, 1451, 1825
BtsI GCAGTG 1 cut(s) 1464
BtsIMutI CAGTG 5 cut(s) 184, 293, 430, 1464, 2274
CaiI CAGNNNCTG 1 cut(s) 1991
CciI TCATGA 1 cut(s) 2286
CfoI GCGC 2 cut(s) 1545, 2025
Cfr10I RCCGGY 1 cut(s) 362
Cfr13I GGNCC 6 cut(s) 136, 365, 575, 836, 1020, 1195
ClaI ATCGAT 1 cut(s) 2128
CsiI ACCWGGT 1 cut(s) 1961
Csp6I GTAC 6 cut(s) 403, 914, 1549, 1724, 1959, 2141
CspCI CAANNNNNGTGG 4 cut(s) 160, 195, 1573, 1608
CviQI GTAC 6 cut(s) 403, 914, 1549, 1724, 1959, 2141
DdeI CTNAG 6 cut(s) 123, 209, 296, 542, 1356, 1539
DpnI GATC 8 cut(s) 633, 969, 1037, 1158, 1417, 1434, 1440, 2131
DpnII GATC 8 cut(s) 631, 967, 1035, 1156, 1415, 1432, 1438, 2129
DraI TTTAAA 1 cut(s) 1479
DriI GACNNNNNGTC 1 cut(s) 506
EaeI YGGCCR 3 cut(s) 340, 412, 589
Eam1104I CTCTTC 5 cut(s) 108, 126, 432, 1056, 2211
Eam1105I GACNNNNNGTC 1 cut(s) 506
EarI CTCTTC 5 cut(s) 108, 126, 432, 1056, 2211
Eco130I CCWWGG 2 cut(s) 387, 956
Eco24I GRGCYC 1 cut(s) 2344
Eco31I GGTCTC 2 cut(s) 2171, 2187
Eco47I GGWCC 2 cut(s) 575, 1195
Eco47III AGCGCT 1 cut(s) 1544
Eco57I CTGAAG 3 cut(s) 132, 456, 1239
Eco81I CCTNAGG 1 cut(s) 209
EcoNI CCTNNNNNAGG 1 cut(s) 2118
EcoO109I RGGNCCY 2 cut(s) 575, 1195
EcoRII CCWGG 4 cut(s) 1604, 1713, 1744, 1961
EcoT14I CCWWGG 2 cut(s) 387, 956
EcoT22I ATGCAT 1 cut(s) 762
EcoT38I GRGCYC 1 cut(s) 2344
ErhI CCWWGG 2 cut(s) 387, 956
FalI AAGNNNNNCTT 2 cut(s) 1272, 1304
FaqI GGGAC 4 cut(s) 513, 1181, 1181, 2193
FauI CCCGC 2 cut(s) 2170, 2355
FauNDI CATATG 2 cut(s) 395, 1954
FbaI TGATCA 2 cut(s) 631, 1035
Fnu4HI GCNGC 6 cut(s) 1623, 1986, 1989, 2016, 2217, 2313
FokI GGATG 6 cut(s) 110, 747, 802, 939, 1458, 1832
FriOI GRGCYC 1 cut(s) 2344
Fsp4HI GCNGC 6 cut(s) 1623, 1986, 1989, 2016, 2217, 2313
FspBI CTAG 3 cut(s) 1329, 1844, 2380
FspI TGCGCA 1 cut(s) 2024
GlaI GCGC 2 cut(s) 1544, 2024
GluI GCNGC 6 cut(s) 1623, 1986, 1989, 2016, 2217, 2313
GsaI CCCAGC 1 cut(s) 2266
GsuI CTGGAG 2 cut(s) 225, 2298
HaeII RGCGCY 1 cut(s) 1546
HaeIII GGCC 8 cut(s) 137, 342, 349, 366, 414, 591, 837, 1021
HapII CCGG 4 cut(s) 363, 1199, 1753, 1817
HhaI GCGC 2 cut(s) 1545, 2025
Hin6I GCGC 2 cut(s) 1543, 2023
HinP1I GCGC 2 cut(s) 1543, 2023
HindIII AAGCTT 2 cut(s) 8, 1281
HinfI GANTC 7 cut(s) 32, 92, 625, 736, 1553, 1861, 2280
HpaII CCGG 4 cut(s) 363, 1199, 1753, 1817
HphI GGTGA 5 cut(s) 194, 1391, 1535, 1771, 2264
Hpy166II GTNNAC 4 cut(s) 310, 796, 1422, 1698
Hpy188I TCNGA 9 cut(s) 71, 97, 480, 569, 1040, 1432, 1459, 1513, 1650
Hpy188III TCNNGA 8 cut(s) 110, 629, 705, 1177, 1253, 1436, 2117, 2287
Hpy8I GTNNAC 4 cut(s) 310, 796, 1422, 1698
Hpy99I CGWCG 1 cut(s) 512
HpyCH4III ACNGT 4 cut(s) 425, 638, 1094, 1728
HpyCH4IV ACGT 2 cut(s) 507, 1870
HpyF10VI GCNNNNNNNGC 8 cut(s) 329, 359, 1280, 1289, 1398, 1622, 1715, 2183
HpyF3I CTNAG 6 cut(s) 123, 209, 296, 542, 1356, 1539
HpySE526I ACGT 2 cut(s) 507, 1870
HspAI GCGC 2 cut(s) 1543, 2023
KflI GGGWCCC 1 cut(s) 1195
KpnI GGTACC 1 cut(s) 1962
KroI GCCGGC 1 cut(s) 362
KroNI GCCGGC 1 cut(s) 364
Ksp22I TGATCA 2 cut(s) 631, 1035
Kzo9I GATC 8 cut(s) 631, 967, 1035, 1156, 1415, 1432, 1438, 2129
LguI GCTCTTC 3 cut(s) 108, 432, 1056
Lsp1109I GCAGC 6 cut(s) 1634, 1997, 2000, 2027, 2228, 2324
LweI GCATC 6 cut(s) 88, 280, 451, 769, 1192, 1224
MabI ACCWGGT 1 cut(s) 1961
MaeI CTAG 3 cut(s) 1329, 1844, 2380
MaeII ACGT 2 cut(s) 507, 1870
MaeIII GTNAC 6 cut(s) 182, 461, 515, 643, 1057, 1379
MalI GATC 8 cut(s) 633, 969, 1037, 1158, 1417, 1434, 1440, 2131
MboI GATC 8 cut(s) 631, 967, 1035, 1156, 1415, 1432, 1438, 2129
MfeI CAATTG 1 cut(s) 901
MflI RGATCY 2 cut(s) 967, 1156
MhlI GDGCHC 2 cut(s) 1700, 2344
MlsI TGGCCA 3 cut(s) 342, 414, 591
MluNI TGGCCA 3 cut(s) 342, 414, 591
MlyI GAGTC 1 cut(s) 2289
MmeI TCCRAC 2 cut(s) 49, 1673
Mox20I TGGCCA 3 cut(s) 342, 414, 591
Mph1103I ATGCAT 1 cut(s) 762
MroNI GCCGGC 1 cut(s) 362
MroXI GAANNNNTTC 1 cut(s) 221
MscI TGGCCA 3 cut(s) 342, 414, 591
MseI TTAA 7 cut(s) 12, 420, 858, 1478, 1643, 1731, 2232
MslI CAYNNNNRTG 7 cut(s) 398, 480, 554, 639, 803, 924, 1686
Msp20I TGGCCA 3 cut(s) 342, 414, 591
MspA1I CMGCKG 1 cut(s) 1991
MspCI CTTAAG 1 cut(s) 11
MspI CCGG 4 cut(s) 363, 1199, 1753, 1817
MspR9I CCNGG 5 cut(s) 1199, 1606, 1715, 1746, 1963
MunI CAATTG 1 cut(s) 901
MvaI CCWGG 4 cut(s) 1606, 1715, 1746, 1963
MwoI GCNNNNNNNGC 8 cut(s) 329, 359, 1280, 1289, 1398, 1622, 1715, 2183
NaeI GCCGGC 1 cut(s) 364
NciI CCSGG 1 cut(s) 1199
NdeI CATATG 2 cut(s) 395, 1954
NdeII GATC 8 cut(s) 631, 967, 1035, 1156, 1415, 1432, 1438, 2129
NgoMIV GCCGGC 1 cut(s) 362
NlaIV GGNNCC 6 cut(s) 1196, 1197, 1536, 1611, 1960, 2367
NmeAIII GCCGAG 1 cut(s) 17
NmuCI GTSAC 5 cut(s) 182, 515, 643, 1057, 1379
NsbI TGCGCA 1 cut(s) 2024
NsiI ATGCAT 1 cut(s) 762
NspI RCATGY 3 cut(s) 802, 1396, 2302
NspV TTCGAA 1 cut(s) 1864
OliI CACNNNNGTG 1 cut(s) 639
PaeI GCATGC 2 cut(s) 1396, 2302
PagI TCATGA 1 cut(s) 2286
PciSI GCTCTTC 3 cut(s) 108, 432, 1056
PdiI GCCGGC 1 cut(s) 364
PdmI GAANNNNTTC 1 cut(s) 221
PfeI GAWTC 6 cut(s) 32, 92, 625, 736, 1553, 1861
PfoI TCCNGGA 1 cut(s) 1197
PkrI GCNGC 6 cut(s) 1624, 1987, 1990, 2017, 2218, 2314
PleI GAGTC 1 cut(s) 2288
PpsI GAGTC 1 cut(s) 2288
PpuMI RGGWCCY 2 cut(s) 575, 1195
PshBI ATTAAT 1 cut(s) 2232
PsiI TTATAA 1 cut(s) 2228
Psp5II RGGWCCY 2 cut(s) 575, 1195
Psp6I CCWGG 4 cut(s) 1604, 1713, 1744, 1961
PspFI CCCAGC 1 cut(s) 2262
PspGI CCWGG 4 cut(s) 1604, 1713, 1744, 1961
PspN4I GGNNCC 6 cut(s) 1196, 1197, 1536, 1611, 1960, 2367
PspPI GGNCC 6 cut(s) 136, 365, 575, 836, 1020, 1195
PspPPI RGGWCCY 2 cut(s) 575, 1195
PsrI GAACNNNNNNTAC 2 cut(s) 1082, 1114
PstI CTGCAG 1 cut(s) 1471
PstNI CAGNNNCTG 1 cut(s) 1991
PsuI RGATCY 2 cut(s) 967, 1156
PvuII CAGCTG 1 cut(s) 1991
RsaI GTAC 6 cut(s) 404, 915, 1550, 1725, 1960, 2142
RsaNI GTAC 6 cut(s) 403, 914, 1549, 1724, 1959, 2141
RseI CAYNNNNRTG 7 cut(s) 398, 480, 554, 639, 803, 924, 1686
SapI GCTCTTC 3 cut(s) 108, 432, 1056
SaqAI TTAA 7 cut(s) 12, 420, 858, 1478, 1643, 1731, 2232
SatI GCNGC 6 cut(s) 1623, 1986, 1989, 2016, 2217, 2313
Sau3AI GATC 8 cut(s) 631, 967, 1035, 1156, 1415, 1432, 1438, 2129
Sau96I GGNCC 6 cut(s) 136, 365, 575, 836, 1020, 1195
ScaI AGTACT 1 cut(s) 1725
SchI GAGTC 1 cut(s) 2289
ScrFI CCNGG 5 cut(s) 1199, 1606, 1715, 1746, 1963
SduI GDGCHC 2 cut(s) 1700, 2344
SexAI ACCWGGT 1 cut(s) 1961
SfaNI GCATC 6 cut(s) 88, 280, 451, 769, 1192, 1224
SfcI CTRYAG 2 cut(s) 1467, 1577
SfuI TTCGAA 1 cut(s) 1864
SinI GGWCC 2 cut(s) 575, 1195
SmiMI CAYNNNNRTG 7 cut(s) 398, 480, 554, 639, 803, 924, 1686
SmlI CTYRAG 1 cut(s) 11
SmoI CTYRAG 1 cut(s) 11
SphI GCATGC 2 cut(s) 1396, 2302
SsiI CCGC 8 cut(s) 160, 353, 1399, 2030, 2096, 2177, 2356, 2362
SspMI CTAG 3 cut(s) 1329, 1844, 2380
StyD4I CCNGG 5 cut(s) 1197, 1604, 1713, 1744, 1961
StyI CCWWGG 2 cut(s) 387, 956
TaaI ACNGT 4 cut(s) 425, 638, 1094, 1728
TaiI ACGT 2 cut(s) 510, 1873
TaqI TCGA 7 cut(s) 510, 680, 1096, 1499, 1864, 2118, 2128
TatI WGTACW 3 cut(s) 402, 1723, 2140
TfiI GAWTC 6 cut(s) 32, 92, 625, 736, 1553, 1861
Tru1I TTAA 7 cut(s) 12, 420, 858, 1478, 1643, 1731, 2232
Tru9I TTAA 7 cut(s) 12, 420, 858, 1478, 1643, 1731, 2232
TscAI CASTG 5 cut(s) 184, 300, 430, 1471, 2281
TseFI GTSAC 5 cut(s) 182, 515, 643, 1057, 1379
TseI GCWGC 6 cut(s) 1622, 1985, 1988, 2015, 2216, 2312
Tsp45I GTSAC 5 cut(s) 182, 515, 643, 1057, 1379
TspGWI ACGGA 1 cut(s) 1319
TspRI CASTG 5 cut(s) 184, 300, 430, 1471, 2281
Vha464I CTTAAG 1 cut(s) 11
VneI GTGCAC 1 cut(s) 1696
VpaK11BI GGWCC 2 cut(s) 575, 1195
VspI ATTAAT 1 cut(s) 2232
XagI CCTNNNNNAGG 1 cut(s) 2118
XapI RAATTY 1 cut(s) 453
XceI RCATGY 3 cut(s) 802, 1396, 2302
XcmI CCANNNNNNNNNTGG 2 cut(s) 771, 1114
XmnI GAANNNNTTC 1 cut(s) 221
XspI CTAG 3 cut(s) 1329, 1844, 2380
ZrmI AGTACT 1 cut(s) 1725
Zsp2I ATGCAT 1 cut(s) 762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.