Rw2G039260

Belongs to the terpene cyclase mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000173
Physical Location & Seq
Forward (+)
64231308 .. 64237854
6547 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G039260.1

Sequence Viewer

Length: 2313 bp
ATGTGGAAGCTTAAGGTTGCAGAAGGTGGGGAAGATTCCTCGGCTTACATTTACAGCACGAACAACTTTGTGGGAAGGCAGACATGGGAGTTTGATCCTGATGCAGGGACTGCTGAAGAGCGAGCTGAGGTTGAAGAGGCTCGTCTTCATTTCTACAACAACCGCCATCAGGTTAAGCCCAGTGGTGACCTTCTTTGGAGATTGCAGTTCCTAAGGGAGAAGCAGTTCAAACAGACAATTCCTCCAATAAAAATTGAAGATGGCGAGGTTATCACATATGAAAAAACCACAGATGCATTGAGAAGGTCTGTCCACTTCTTCTCAGCGCTGCAAGCAAGCGATGGCCATTGGCCTGCGGAAAATGCTGGCCCTTTATTTTTCCTTCCTCCTATGGTCATAGTTATGCACATTACGGGGCATCTTAACAGTGTATTCTCTGAGGAGCATCGCAAAGAAATTCTGCGTTACATATACTATCATCAGAATGAAGATGGTGGTTGGGGACTACATGTCGAAGGTCATAGCACCATGTTCAGCACAGCTCTCAGCTACATTTGTATGCGTATTCTCGGAGAAGGACCTGATGATGGTGGCCAAGACAATGCTTGTCCGAGAGCAAGAAAGTGGATTCTTGATCATGGTGGTGTCACACACATACCCTCTTGGGGAAAGACTTGGCTTTCGATACTTGGTTTGTTTAATTGGTCTGGAAGCAACCCTATTCCCCCAGAGTTTTGGACTATTCCTTCATTTCTTCCTATGCATCCAGCAAAAATGTGGTGCTATAGTAGGACGTCTTACATGCCAATGTCATACCTATATGGAAAAAGGTTTGTTGGCCCAATTACACCTCTCATTCTTCAATTGAGGGACGAACTTTTTATTCAACCTTACACTGAAATCAATTGGAAGGAAGTACGCCATCTATGTGCCAAAGAGGATATCTACTACCCTCATCCTTGGATACAGGATCTCCTTTGGGATACCCTCTACATTTCCACAGAGCCTCTTCTTACCCGTTGGCCTTTCAACAAGTTGATCAGAGAAAAGGCTCTACAAGTGACGATGGAGCATATTCATTACGAAGATGAGAACAGCCGATACATTACCATGGGGTGTTTGGAAAAGGTGTTATGTATGCTTGCCTGTTGGGCTGAAGATCCAAATGGGGACTATTTCAAGAAGCATCTCGCTAGGATCCCGGATTATTTATGGGTTGCTGAAGATGGGATGAAGATGCAAAGTTTTGGCAGTCAGGAGTGGGATACTAGTTTAGCCATTCAAGCTTTGCTTGCTAGTAATCTCAAGGATGAAATTGGACCAACGCTTGCTAGAGGACATGACTTCATAAAGAAATCTCAGGTCAAGGACAACCCTTCTGGTGACTTCAAAAGCATGTACCGCCACATTTCTAAAGGATCGTGGACTTTCTCCGATCAAGATCATGGATGGCAAGTTTCTGATTGCACTGCAGAAGGTTTAAAGTGTTGTCTTCTGTTGTCGATGATGCCATCGGAGATAGTTGGTGAAAAAATGGAGCCTGAGCGCTTGTACGATTCTGTCAATGTTCTTCTTTCCCTACAGAGCAAGAATGGTGGTTTTTCAGCCTGGGAGCCAGCAGGGGCAGCAGAATGGTTAGAACTGTTAAATCCGACAGAGTTCTTGGAGGACATTGTAATTGAGCATGAACATGTTGAGTGCACTTCATCTGTAATCCAGGCTTTAGTTCTGTTCAAGAAGCTACACCCTGGGCATAGGAAGAAAGAGATTGAACATTCCATCACCAATGCTGTACGCTACGTTGAAAATATGCAAATGCCGGATGGTTCATGGTATGGAGATTGGGGAGTTTGCTTCACATATGGTACCTGGTTTGCACTAGGAGGATTGGCAGCTGCTGGCAAGACTTTTAGCACTTGTGCAGCCATGCGCAATGGGGTTAGCTTTCTACTTACGGTGCAGAGAGAGAACGGTGGTTGGGGAGAGAGTTATCTTTCATGTCCACAAAAGACATACATTCCTCTCGAAGGAAACCGATCCAATCTAGTACATACTGCTTGGGCTATGATGGGTCTCATTCATGCGGGACAGGCAGAAAGAGACCCAACACCTCTTCATCGCGCAGCAAAGTTTATAATCAATTCTCAACTGGAAAATGGTGATTTTCCCCAGCAGGAAATCACTGGAGTCTTCAAGAAGAACTGCATGCTACATTATGCAGCATATAGAAAATATTCCACTGTGGGCTCTTGCAGAATATCGCAATTGTTTTCCCTTGCCTTCCAAGGGCTAAGGGGCTCCCCATATATGTAG

Protein Analysis

770

Amino Acids

88.09

Weight (kDa)

5.93

Isoelectric Point (pI)

45.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQHop_cyclase_N PF13249 101 - 401 4.5e-37 Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C PF13243 420 - 745 4.2e-46 Squalene-hopene cyclase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66960 AT1G66960 AT1G66960 AT1G66960 AT1G78950 AT1G78950 AT1G78955 AT1G78955 AT1G78955 AT1G78955 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78970 AT1G78970 AT1G78970
fragaria_vesca FvH4_5g29480 FvH4_6g36450 FvH4_6g36450 FvH4_6g36500 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g37620
malus_domestica MD03G1089600.v1.1 MD09G1167700.v1.1 MD09G1168200.v1.1 MD09G1168300.v1.1 MD11G1098600.v1.1 MD17G1158300.v1.1 MD17G1158700.v1.1 MD17G1159500.v1.1 MD17G1160500.v1.1 MD17G1182500.v1.1 MD17G1182700.v1.1 MD17G1245800.v1.1 MD17G1246300.v1.1 MD17G1246600.v1.1
prunus_persica Prupe.3G025700_v2.0.a1 Prupe.3G025700_v2.0.a1 Prupe.3G025800_v2.0.a1 Prupe.3G025900_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026400_v2.0.a1 Prupe.3G026500_v2.0.a1 Prupe.3G026700_v2.0.a1 Prupe.3G026800_v2.0.a1
pyrus_communis pycom03g07120 pycom09g08520 pycom09g08590 pycom09g08610 pycom10g10070 pycom11g08310 pycom16g19880 pycom17g15190 pycom17g15300
rosa_chinensis RchiOBHm_Chr2g0136221 RchiOBHm_Chr2g0148451 RchiOBHm_Chr2g0148501 RchiOBHm_Chr2g0148521 RchiOBHm_Chr2g0148661 RchiOBHm_Chr2g0148701 RchiOBHm_Chr2g0148811 RchiOBHm_Chr2g0148941 RchiOBHm_Chr2g0148981 RchiOBHm_Chr2g0150451 RchiOBHm_Chr2g0150521 RchiOBHm_Chr2g0150611 RchiOBHm_Chr4g0420831 RchiOBHm_Chr5g0049791 RchiOBHm_Chr5g0049801
rosa_laevigata RLG00000007735 RLG00000020351 RLG00000020352 RLG00000020357 RLG00000020358 RLG00000020360 RLG00000029093 RLG00000030686
rosa_multiflora Rmu_co8274513.1_g000001 Rmu_sc0000048.1_g000003 Rmu_sc0000048.1_g000033 Rmu_sc0000048.1_g000039 Rmu_sc0001397.1_g000011 Rmu_sc0001998.1_g000039 Rmu_sc0002070.1_g000055 Rmu_sc0002082.1_g000052 Rmu_sc0003181.1_g000010 Rmu_sc0005104.1_g000005 Rmu_sc0006758.1_g000002 Rmu_sc0008411.1_g000029 Rmu_sc0008411.1_g000030 Rmu_sc0017472.1_g000001 Rmu_sc0017472.1_g000003 Rmu_sc0029876.1_g000001 Rmu_ssc0000480.1_g000024 Rmu_ssc0000480.1_g000036
rosa_roxburghii Rroxscaffold_2G00097070 Rroxscaffold_2G00098540 Rroxscaffold_2G00098560 Rroxscaffold_2G00098570 Rroxscaffold_2G00098650 Rroxscaffold_2G00098710 Rroxscaffold_2G00098740 Rroxscaffold_3G00225830 Rroxscaffold_3G00236870 Rroxscaffold_4G00312240
rosa_rugosa Rorug01G0047000 Rorug02G0372300 Rorug02G0407200 Rorug02G0407300 Rorug02G0408400 Rorug02G0421500 Rorug07G0208900
rosa_samantha Rh2AG380200 Rh2AG465700 Rh2AG465800 Rh2AG466800 Rh2AG467100 Rh2AG467600 Rh2AG480900 Rh2AG481400 Rh2AG481700 Rh2CG073500 Rh2CG452200 Rh2CG453200 Rh2CG453600 Rh2CG454100 Rh2CG467100 Rh2CG467800 Rh2CG468000 Rh2DG403100 Rh2DG487800 Rh2DG487900 Rh2DG488800 Rh2DG502000 Rh2DG502200 Rh2DG502300 Rh2DG503100 Rh3AG319300 Rh4AG227700 Rh4BG371800 Rh5AG327400 Rh5DG350600 Rh7AG349800
rosa_wichuraiana Rw0G010080 Rw2G031110 Rw2G037980 Rw2G037990 Rw2G038000 Rw2G038010 Rw2G038080 Rw2G038100 Rw2G039260 Rw2G039290 Rw2G039470 Rw4G019710 Rw5G030930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2135
AatII GACGTC 1 cut(s) 797
Acc16I TGCGCA 1 cut(s) 1931
Acc65I GGTACC 1 cut(s) 1865
AccB1I GGYRCC 1 cut(s) 1865
AccII CGCG 1 cut(s) 2121
AciI CCGC 4 cut(s) 163, 356, 1402, 2084
AclWI GGATC 7 cut(s) 89, 978, 1154, 1192, 1205, 1426, 2031
AcoI YGGCCR 2 cut(s) 343, 592
AcsI RAATTY 1 cut(s) 456
AcuI CTGAAG 3 cut(s) 135, 1176, 1242
AcyI GRCGYC 1 cut(s) 794
AfaI GTAC 6 cut(s) 918, 1400, 1553, 1794, 1867, 2049
AfeI AGCGCT 2 cut(s) 327, 1547
AfiI CCNNNNNNNGG 6 cut(s) 104, 169, 587, 665, 2027, 2286
AflII CTTAAG 1 cut(s) 11
AflIII ACRYGT 2 cut(s) 508, 1690
AhdI GACNNNNNGTC 1 cut(s) 509
AhlI ACTAGT 1 cut(s) 1268
AjnI CCWGG 4 cut(s) 1607, 1716, 1747, 1868
AloI GAACNNNNNNTCC 2 cut(s) 209, 241
AluBI AGCT 8 cut(s) 10, 125, 542, 549, 1286, 1741, 1895, 1944
AluI AGCT 8 cut(s) 10, 125, 542, 549, 1286, 1741, 1895, 1944
Alw21I GWGCWC 1 cut(s) 1703
Alw26I GTCTC 2 cut(s) 2078, 2094
Alw44I GTGCAC 1 cut(s) 1699
AlwI GGATC 7 cut(s) 89, 978, 1154, 1192, 1205, 1426, 2031
AlwNI CAGNNNCTG 2 cut(s) 110, 1898
Aor51HI AGCGCT 2 cut(s) 327, 1547
AoxI GGCC 6 cut(s) 343, 350, 367, 592, 838, 1022
ApaLI GTGCAC 1 cut(s) 1699
ApeKI GCWGC 7 cut(s) 328, 1625, 1892, 1895, 1922, 2123, 2219
ApoI RAATTY 1 cut(s) 456
ArsI GACNNNNNNTTYG 2 cut(s) 664, 696
Asp700I GAANNNNTTC 2 cut(s) 224, 2233
Asp718I GGTACC 1 cut(s) 1865
AspLEI GCGC 4 cut(s) 328, 1548, 1932, 2123
AspS9I GGNCC 4 cut(s) 368, 578, 839, 1319
AsuC2I CCSGG 1 cut(s) 1202
AsuHPI GGTGA 5 cut(s) 197, 1394, 1538, 1774, 2171
AvaII GGWCC 2 cut(s) 578, 1319
AxyI CCTNAGG 1 cut(s) 212
BaeGI GKGCMC 1 cut(s) 1703
BalI TGGCCA 2 cut(s) 345, 594
BamHI GGATCC 1 cut(s) 1197
BanI GGYRCC 1 cut(s) 1865
BanII GRGCYC 2 cut(s) 2249, 2300
BbsI GAAGAC 3 cut(s) 137, 1484, 2182
Bbv12I GWGCWC 1 cut(s) 1703
BbvCI CCTCAGC 1 cut(s) 126
BbvI GCAGC 7 cut(s) 315, 1637, 1882, 1904, 1934, 2135, 2231
BciT130I CCWGG 4 cut(s) 1609, 1718, 1749, 1870
BciVI GTATCC 3 cut(s) 957, 976, 1258
BclI TGATCA 2 cut(s) 634, 1038
BcnI CCSGG 1 cut(s) 1202
BcoDI GTCTC 2 cut(s) 2078, 2094
BcuI ACTAGT 1 cut(s) 1268
BfaI CTAG 6 cut(s) 1194, 1269, 1296, 1332, 1880, 2045
BfmI CTRYAG 3 cut(s) 784, 1470, 1580
BfoI RGCGCY 2 cut(s) 329, 1549
BfrI CTTAAG 1 cut(s) 11
BfuI GTATCC 3 cut(s) 957, 976, 1258
BglI GCCNNNNNGGC 1 cut(s) 1151
BisI GCNGC 7 cut(s) 329, 1626, 1893, 1896, 1923, 2124, 2220
BlsI GCNGC 7 cut(s) 330, 1627, 1894, 1897, 1924, 2125, 2221
Bme1390I CCNGG 5 cut(s) 1202, 1609, 1718, 1749, 1870
Bme18I GGWCC 2 cut(s) 578, 1319
BmeRI GACNNNNNGTC 1 cut(s) 509
BmgT120I GGNCC 4 cut(s) 368, 578, 839, 1319
BmiI GGNNCC 5 cut(s) 1199, 1539, 1614, 1867, 2299
BmrFI CCNGG 5 cut(s) 1202, 1609, 1718, 1749, 1870
BmrI ACTGGG 1 cut(s) 174
BmsI GCATC 8 cut(s) 91, 283, 427, 454, 772, 1195, 1227, 1497
BmuI ACTGGG 1 cut(s) 174
BpiI GAAGAC 3 cut(s) 137, 1484, 2182
BpmI CTGGAG 1 cut(s) 2205
Bpu10I CCTNAGC 3 cut(s) 126, 1542, 2291
BpuEI CTTGAG 1 cut(s) 1289
BpuMI CCSGG 1 cut(s) 1202
BsaBI GATNNNNATC 1 cut(s) 1440
BsaHI GRCGYC 1 cut(s) 794
BsaI GGTCTC 2 cut(s) 2078, 2094
BsaJI CCNNGG 7 cut(s) 39, 959, 1110, 1608, 1747, 1748, 2284
BsaXI ACNNNNNCTCC 6 cut(s) 209, 226, 239, 256, 957, 987
Bsc4I CCNNNNNNNGG 6 cut(s) 104, 169, 587, 665, 2027, 2286
Bse1I ACTGG 3 cut(s) 180, 2154, 2188
Bse21I CCTNAGG 1 cut(s) 212
Bse3DI GCAATG 1 cut(s) 1939
Bse8I GATNNNNATC 1 cut(s) 1440
BseBI CCWGG 4 cut(s) 1609, 1718, 1749, 1870
BseDI CCNNGG 7 cut(s) 39, 959, 1110, 1608, 1747, 1748, 2284
BseGI GGATG 6 cut(s) 763, 955, 1236, 1315, 1454, 1828
BseJI GATNNNNATC 1 cut(s) 1440
BseLI CCNNNNNNNGG 6 cut(s) 104, 169, 587, 665, 2027, 2286
BseMI GCAATG 1 cut(s) 1939
BseMII CTCAG 6 cut(s) 117, 336, 429, 559, 1373, 1533
BseNI ACTGG 3 cut(s) 180, 2154, 2188
BseRI GAGGAG 1 cut(s) 455
BseSI GKGCMC 1 cut(s) 1703
BseXI GCAGC 7 cut(s) 315, 1637, 1882, 1904, 1934, 2135, 2231
BseYI CCCAGC 1 cut(s) 2169
BsgI GTGCAG 2 cut(s) 1941, 1979
Bsh1236I CGCG 1 cut(s) 2121
BshFI GGCC 6 cut(s) 345, 352, 369, 594, 840, 1024
BshNI GGYRCC 1 cut(s) 1865
BsiHKAI GWGCWC 1 cut(s) 1703
BsiSI CCGG 2 cut(s) 1202, 1820
BslFI GGGAC 5 cut(s) 121, 516, 884, 1184, 2100
BslI CCNNNNNNNGG 6 cut(s) 104, 169, 587, 665, 2027, 2286
BsmAI GTCTC 2 cut(s) 2078, 2094
BsmFI GGGAC 5 cut(s) 121, 516, 884, 1184, 2100
BsnI GGCC 6 cut(s) 345, 352, 369, 594, 840, 1024
Bso31I GGTCTC 2 cut(s) 2078, 2094
Bsp1286I GDGCHC 3 cut(s) 1703, 2249, 2300
Bsp19I CCATGG 1 cut(s) 1110
BspACI CCGC 4 cut(s) 163, 356, 1402, 2084
BspANI GGCC 6 cut(s) 345, 352, 369, 594, 840, 1024
BspCNI CTCAG 6 cut(s) 118, 335, 430, 558, 1372, 1534
BspFNI CGCG 1 cut(s) 2121
BspLI GGNNCC 5 cut(s) 1199, 1539, 1614, 1867, 2299
BspMAI CTGCAG 1 cut(s) 1474
BspPI GGATC 7 cut(s) 89, 978, 1154, 1192, 1205, 1426, 2031
BspQI GCTCTTC 1 cut(s) 111
BspT107I GGYRCC 1 cut(s) 1865
BspTI CTTAAG 1 cut(s) 11
BspTNI GGTCTC 2 cut(s) 2078, 2094
BsrDI GCAATG 1 cut(s) 1939
BsrI ACTGG 3 cut(s) 180, 2154, 2188
BssECI CCNNGG 7 cut(s) 39, 959, 1110, 1608, 1747, 1748, 2284
BssNI GRCGYC 1 cut(s) 794
BssT1I CCWWGG 3 cut(s) 959, 1110, 2284
Bst2UI CCWGG 4 cut(s) 1609, 1718, 1749, 1870
Bst4CI ACNGT 5 cut(s) 428, 1644, 1957, 1973, 2242
Bst6I CTCTTC 4 cut(s) 111, 129, 1014, 2118
BstACI GRCGYC 1 cut(s) 794
BstAFI CTTAAG 1 cut(s) 11
BstAPI GCANNNNNTGC 1 cut(s) 110
BstDEI CTNAG 8 cut(s) 126, 212, 322, 438, 545, 1359, 1542, 2291
BstDSI CCRYGG 1 cut(s) 1110
BstEII GGTNACC 1 cut(s) 185
BstENI CCTNNNNNAGG 2 cut(s) 102, 2025
BstF5I GGATG 6 cut(s) 763, 955, 1236, 1315, 1454, 1828
BstFNI CGCG 1 cut(s) 2121
BstH2I RGCGCY 2 cut(s) 329, 1549
BstHHI GCGC 4 cut(s) 328, 1548, 1932, 2123
BstMAI GTCTC 2 cut(s) 2078, 2094
BstMWI GCNNNNNNNGC 9 cut(s) 110, 332, 362, 1151, 1283, 1292, 1401, 1625, 2090
BstNI CCWGG 4 cut(s) 1609, 1718, 1749, 1870
BstNSI RCATGY 5 cut(s) 512, 805, 1399, 1694, 2209
BstPI GGTNACC 1 cut(s) 185
BstSCI CCNGG 5 cut(s) 1200, 1607, 1716, 1747, 1868
BstSFI CTRYAG 3 cut(s) 784, 1470, 1580
BstSLI GKGCMC 1 cut(s) 1703
BstUI CGCG 1 cut(s) 2121
BstV1I GCAGC 7 cut(s) 315, 1637, 1882, 1904, 1934, 2135, 2231
BstV2I GAAGAC 3 cut(s) 137, 1484, 2182
BstX2I RGATCY 3 cut(s) 970, 1159, 1197
BstXI CCANNNNNNTGG 1 cut(s) 735
BstYI RGATCY 3 cut(s) 970, 1159, 1197
Bsu36I CCTNAGG 1 cut(s) 212
BsuI GTATCC 3 cut(s) 957, 976, 1258
BsuRI GGCC 6 cut(s) 345, 352, 369, 594, 840, 1024
BtgI CCRYGG 1 cut(s) 1110
BtgZI GCGATG 3 cut(s) 354, 431, 2102
BtsCI GGATG 6 cut(s) 763, 955, 1236, 1315, 1454, 1828
BtsI GCAGTG 1 cut(s) 1467
BtsIMutI CAGTG 6 cut(s) 187, 433, 894, 1467, 2181, 2238
CaiI CAGNNNCTG 2 cut(s) 110, 1898
CfoI GCGC 4 cut(s) 328, 1548, 1932, 2123
Cfr13I GGNCC 4 cut(s) 368, 578, 839, 1319
CsiI ACCWGGT 1 cut(s) 1868
Csp6I GTAC 6 cut(s) 917, 1399, 1552, 1793, 1866, 2048
CspCI CAANNNNNGTGG 2 cut(s) 1576, 1611
CviQI GTAC 6 cut(s) 917, 1399, 1552, 1793, 1866, 2048
DdeI CTNAG 8 cut(s) 126, 212, 322, 438, 545, 1359, 1542, 2291
DraI TTTAAA 1 cut(s) 1482
DriI GACNNNNNGTC 1 cut(s) 509
EaeI YGGCCR 2 cut(s) 343, 592
Eam1104I CTCTTC 4 cut(s) 111, 129, 1014, 2118
Eam1105I GACNNNNNGTC 1 cut(s) 509
EarI CTCTTC 4 cut(s) 111, 129, 1014, 2118
Eco130I CCWWGG 3 cut(s) 959, 1110, 2284
Eco24I GRGCYC 2 cut(s) 2249, 2300
Eco31I GGTCTC 2 cut(s) 2078, 2094
Eco32I GATATC 1 cut(s) 943
Eco47I GGWCC 2 cut(s) 578, 1319
Eco47III AGCGCT 2 cut(s) 327, 1547
Eco57I CTGAAG 3 cut(s) 135, 1176, 1242
Eco81I CCTNAGG 1 cut(s) 212
Eco91I GGTNACC 1 cut(s) 185
EcoNI CCTNNNNNAGG 2 cut(s) 102, 2025
EcoO109I RGGNCCY 1 cut(s) 578
EcoO65I GGTNACC 1 cut(s) 185
EcoRII CCWGG 4 cut(s) 1607, 1716, 1747, 1868
EcoRV GATATC 1 cut(s) 943
EcoT14I CCWWGG 3 cut(s) 959, 1110, 2284
EcoT22I ATGCAT 2 cut(s) 298, 765
EcoT38I GRGCYC 2 cut(s) 2249, 2300
ErhI CCWWGG 3 cut(s) 959, 1110, 2284
FalI AAGNNNNNCTT 2 cut(s) 1275, 1307
FaqI GGGAC 5 cut(s) 121, 516, 884, 1184, 2100
FauI CCCGC 1 cut(s) 2077
FauNDI CATATG 2 cut(s) 277, 1861
FbaI TGATCA 2 cut(s) 634, 1038
Fnu4HI GCNGC 7 cut(s) 329, 1626, 1893, 1896, 1923, 2124, 2220
FokI GGATG 6 cut(s) 750, 942, 1243, 1322, 1461, 1835
FriOI GRGCYC 2 cut(s) 2249, 2300
Fsp4HI GCNGC 7 cut(s) 329, 1626, 1893, 1896, 1923, 2124, 2220
FspBI CTAG 6 cut(s) 1194, 1269, 1296, 1332, 1880, 2045
FspI TGCGCA 1 cut(s) 1931
GlaI GCGC 4 cut(s) 327, 1547, 1931, 2122
GluI GCNGC 7 cut(s) 329, 1626, 1893, 1896, 1923, 2124, 2220
GsaI CCCAGC 1 cut(s) 2173
GsuI CTGGAG 1 cut(s) 2205
HaeII RGCGCY 2 cut(s) 329, 1549
HaeIII GGCC 6 cut(s) 345, 352, 369, 594, 840, 1024
HapII CCGG 2 cut(s) 1202, 1820
HhaI GCGC 4 cut(s) 328, 1548, 1932, 2123
Hin1I GRCGYC 1 cut(s) 794
Hin6I GCGC 4 cut(s) 326, 1546, 1930, 2121
HinP1I GCGC 4 cut(s) 326, 1546, 1930, 2121
HindIII AAGCTT 2 cut(s) 8, 1284
HinfI GANTC 4 cut(s) 35, 628, 1556, 2187
HpaII CCGG 2 cut(s) 1202, 1820
HphI GGTGA 5 cut(s) 197, 1394, 1538, 1774, 2171
Hpy166II GTNNAC 4 cut(s) 313, 1425, 1701, 2003
Hpy188I TCNGA 9 cut(s) 439, 483, 572, 612, 1043, 1435, 1462, 1516, 1653
Hpy188III TCNNGA 9 cut(s) 98, 632, 708, 1180, 1256, 1439, 1735, 2024, 2194
Hpy8I GTNNAC 4 cut(s) 313, 1425, 1701, 2003
HpyCH4III ACNGT 5 cut(s) 428, 1644, 1957, 1973, 2242
HpyCH4IV ACGT 2 cut(s) 794, 1800
HpyF10VI GCNNNNNNNGC 9 cut(s) 110, 332, 362, 1151, 1283, 1292, 1401, 1625, 2090
HpyF3I CTNAG 8 cut(s) 126, 212, 322, 438, 545, 1359, 1542, 2291
HpySE526I ACGT 2 cut(s) 794, 1800
Hsp92I GRCGYC 1 cut(s) 794
HspAI GCGC 4 cut(s) 326, 1546, 1930, 2121
KpnI GGTACC 1 cut(s) 1869
Ksp22I TGATCA 2 cut(s) 634, 1038
LguI GCTCTTC 1 cut(s) 111
LmnI GCTCC 5 cut(s) 442, 1069, 1537, 1612, 2303
Lsp1109I GCAGC 7 cut(s) 315, 1637, 1882, 1904, 1934, 2135, 2231
LweI GCATC 8 cut(s) 91, 283, 427, 454, 772, 1195, 1227, 1497
MabI ACCWGGT 1 cut(s) 1868
MaeI CTAG 6 cut(s) 1194, 1269, 1296, 1332, 1880, 2045
MaeII ACGT 2 cut(s) 794, 1800
MaeIII GTNAC 5 cut(s) 185, 464, 646, 1060, 1382
MfeI CAATTG 3 cut(s) 863, 904, 2264
MflI RGATCY 3 cut(s) 970, 1159, 1197
MhlI GDGCHC 3 cut(s) 1703, 2249, 2300
MlsI TGGCCA 2 cut(s) 345, 594
MluNI TGGCCA 2 cut(s) 345, 594
MlyI GAGTC 1 cut(s) 2196
MmeI TCCRAC 1 cut(s) 1676
Mox20I TGGCCA 2 cut(s) 345, 594
Mph1103I ATGCAT 2 cut(s) 298, 765
MroXI GAANNNNTTC 2 cut(s) 224, 2233
MscI TGGCCA 2 cut(s) 345, 594
MseI TTAA 6 cut(s) 12, 174, 423, 699, 1481, 1646
MslI CAYNNNNRTG 8 cut(s) 401, 483, 557, 642, 806, 927, 1109, 1689
Msp20I TGGCCA 2 cut(s) 345, 594
MspA1I CMGCKG 1 cut(s) 1895
MspCI CTTAAG 1 cut(s) 11
MspI CCGG 2 cut(s) 1202, 1820
MspR9I CCNGG 5 cut(s) 1202, 1609, 1718, 1749, 1870
MunI CAATTG 3 cut(s) 863, 904, 2264
MvaI CCWGG 4 cut(s) 1609, 1718, 1749, 1870
MvnI CGCG 1 cut(s) 2121
MwoI GCNNNNNNNGC 9 cut(s) 110, 332, 362, 1151, 1283, 1292, 1401, 1625, 2090
NciI CCSGG 1 cut(s) 1202
NcoI CCATGG 1 cut(s) 1110
NdeI CATATG 2 cut(s) 277, 1861
NlaIV GGNNCC 5 cut(s) 1199, 1539, 1614, 1867, 2299
NmeAIII GCCGAG 1 cut(s) 20
NmuCI GTSAC 4 cut(s) 185, 646, 1060, 1382
NsbI TGCGCA 1 cut(s) 1931
NsiI ATGCAT 2 cut(s) 298, 765
NspI RCATGY 5 cut(s) 512, 805, 1399, 1694, 2209
PaeI GCATGC 1 cut(s) 2209
PasI CCCWGGG 1 cut(s) 1748
PciI ACATGT 2 cut(s) 508, 1690
PciSI GCTCTTC 1 cut(s) 111
PdmI GAANNNNTTC 2 cut(s) 224, 2233
PfeI GAWTC 3 cut(s) 35, 628, 1556
PfoI TCCNGGA 1 cut(s) 1200
PkrI GCNGC 7 cut(s) 330, 1627, 1894, 1897, 1924, 2125, 2221
PleI GAGTC 1 cut(s) 2195
PpsI GAGTC 1 cut(s) 2195
PpuMI RGGWCCY 1 cut(s) 578
PscI ACATGT 2 cut(s) 508, 1690
PsiI TTATAA 1 cut(s) 2135
Psp5II RGGWCCY 1 cut(s) 578
Psp6I CCWGG 4 cut(s) 1607, 1716, 1747, 1868
PspEI GGTNACC 1 cut(s) 185
PspFI CCCAGC 1 cut(s) 2169
PspGI CCWGG 4 cut(s) 1607, 1716, 1747, 1868
PspN4I GGNNCC 5 cut(s) 1199, 1539, 1614, 1867, 2299
PspPI GGNCC 4 cut(s) 368, 578, 839, 1319
PspPPI RGGWCCY 1 cut(s) 578
PsrI GAACNNNNNNTAC 2 cut(s) 1085, 1117
PstI CTGCAG 1 cut(s) 1474
PstNI CAGNNNCTG 2 cut(s) 110, 1898
PsuI RGATCY 3 cut(s) 970, 1159, 1197
PvuII CAGCTG 1 cut(s) 1895
RsaI GTAC 6 cut(s) 918, 1400, 1553, 1794, 1867, 2049
RsaNI GTAC 6 cut(s) 917, 1399, 1552, 1793, 1866, 2048
RseI CAYNNNNRTG 8 cut(s) 401, 483, 557, 642, 806, 927, 1109, 1689
SapI GCTCTTC 1 cut(s) 111
SaqAI TTAA 6 cut(s) 12, 174, 423, 699, 1481, 1646
SatI GCNGC 7 cut(s) 329, 1626, 1893, 1896, 1923, 2124, 2220
Sau96I GGNCC 4 cut(s) 368, 578, 839, 1319
SchI GAGTC 1 cut(s) 2196
ScrFI CCNGG 5 cut(s) 1202, 1609, 1718, 1749, 1870
SduI GDGCHC 3 cut(s) 1703, 2249, 2300
SexAI ACCWGGT 1 cut(s) 1868
SfaNI GCATC 8 cut(s) 91, 283, 427, 454, 772, 1195, 1227, 1497
SfcI CTRYAG 3 cut(s) 784, 1470, 1580
SinI GGWCC 2 cut(s) 578, 1319
SmiMI CAYNNNNRTG 8 cut(s) 401, 483, 557, 642, 806, 927, 1109, 1689
SmlI CTYRAG 2 cut(s) 11, 1304
SmoI CTYRAG 2 cut(s) 11, 1304
SpeI ACTAGT 1 cut(s) 1268
SphI GCATGC 1 cut(s) 2209
SsiI CCGC 4 cut(s) 163, 356, 1402, 2084
SspI AATATT 1 cut(s) 2234
SspMI CTAG 6 cut(s) 1194, 1269, 1296, 1332, 1880, 2045
StyD4I CCNGG 5 cut(s) 1200, 1607, 1716, 1747, 1868
StyI CCWWGG 3 cut(s) 959, 1110, 2284
TaaI ACNGT 5 cut(s) 428, 1644, 1957, 1973, 2242
TaiI ACGT 2 cut(s) 797, 1803
TaqI TCGA 4 cut(s) 513, 683, 1502, 2025
TatI WGTACW 1 cut(s) 2047
TfiI GAWTC 3 cut(s) 35, 628, 1556
Tru1I TTAA 6 cut(s) 12, 174, 423, 699, 1481, 1646
Tru9I TTAA 6 cut(s) 12, 174, 423, 699, 1481, 1646
TscAI CASTG 6 cut(s) 187, 433, 901, 1474, 2188, 2245
TseFI GTSAC 4 cut(s) 185, 646, 1060, 1382
TseI GCWGC 7 cut(s) 328, 1625, 1892, 1895, 1922, 2123, 2219
Tsp45I GTSAC 4 cut(s) 185, 646, 1060, 1382
TspRI CASTG 6 cut(s) 187, 433, 901, 1474, 2188, 2245
Vha464I CTTAAG 1 cut(s) 11
VneI GTGCAC 1 cut(s) 1699
VpaK11BI GGWCC 2 cut(s) 578, 1319
XagI CCTNNNNNAGG 2 cut(s) 102, 2025
XapI RAATTY 1 cut(s) 456
XceI RCATGY 5 cut(s) 512, 805, 1399, 1694, 2209
XcmI CCANNNNNNNNNTGG 2 cut(s) 774, 1117
XmnI GAANNNNTTC 2 cut(s) 224, 2233
XspI CTAG 6 cut(s) 1194, 1269, 1296, 1332, 1880, 2045
ZraI GACGTC 1 cut(s) 795
Zsp2I ATGCAT 2 cut(s) 298, 765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.