Rroxscaffold_2G00098540

Belongs to the terpene cyclase mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000173
Physical Location & Seq
Reverse (-)
19823016 .. 19827611
4596 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00098540.1

Sequence Viewer

Length: 2136 bp
ATGTGGAGGATTAAGTTCGGAGAGGGTGCCAATGACCCTTTGCTGTTCAGCACAAACAACTTCCATGGGAGGCAGACATGGGAGTTCGACCCCGATGCAGGCACACCGGAAGAGCGTGCCGAGGTTGAGGCCGCTCGTGAGAATTTCTACCAAAATCGCTACAAGGTTGCTCCCAGCAGTGACCTCCTCTGGCGTTTTCAGATGCTAAGAGAGAGAAACTTCAAGCAAGAAATTCCTCCGGTGAGAATTGGGGAAGGTGAGGAAATAACATTTGATCAGGCCACAGCTGCATACAAGAGGGCTGCTTCCTTCTGGAATGCCTTGCAGTCACCCCATGGTCACTGGCCTGCTGAGAATGCTGGTCCAAACTTTTACTTTCCTCCCTTGGTCATGAGTGTATACATACCCGGATACCTCAATGTTGTTTTCTCTGCTGAGCACAAGAAGGAAATCTTGCGCTACACATACAACCATCAGAACGAAGATGGTGGCTGGGAAGGCCCTGAGGGTGGTCGAGACAACGCCTGTGCAAGGGCCCGGAAATGGATTCTTGACCGCGGTGGTGCACAATATTCTGCCTCATGGGGAAAGACCTGGATGGCGGTACTTGGTGTTTATGATTGGGAAGGAAGCAACCCAATGCCCCCAGAGTTTTGGACCTACCCTACTCTGCTTCCATTTCATCCATCAAAAATGTTTTGCTACTGTAGGTTGACTTACCTACCCATGTCTTACTTCTATGCAACAAGATTTGTTGGCCCAATCACTCCTCTTGTTGAGCAATTGAGACAAGAAATCTACAATGAGCCTTACGATCAAATCAAGTGGAGTCAAGTGCGCCATCATTGTGCAACAGAAGATAACTATTATCCCCATGGAAGAGTACAGCGCTTTATGTGGGATAGTCTTTACAATATTTGTGAGCCTCTTCTCAAAATCTGGCCCTTCAAAAAGATCAGAGACAATGCTGTTCAATTCACCATTGACCAAATTCATTACGAAGATGAGAACAGTCGCTACATTACCATTGGATGTGTTGAAAAGCCATTGATGATGCTTGCTTGTTGGGCTGAGGATCCTAGTGGAGAAGCTTTTAAGAAGCATCTTCCTAGAGTTACTGATTATATTTGGCTCGGTGAAGATGGTATCAAGATGCAGAGTTTTGGTAGTCAGTCATGGGATTGTGCTCTTGTGATTCAAGCATTGCTTTGTGGGAATCTCAACAATGAATTTGGACCTGTACTTAAGTTGGCACATGACTTCCTAAAGAAATCTCAGGATGAATACTTCCGGCGACTACTTGGCTCATTTTTCCGTCATATTTCTAAAGGATGCTGGACTTTCTCTGATCGGGATCATGGATGGCAAGTGTCTGACTGCACTGCAGAAGCATTGAGGTGCTGCTGTCTGTTTGCAATGATGTCACCAGAGCTTGTTGGTGAGCCAATGGAAGCTGAGTGTATGTATGATGCTGTCAATGTCATAATGTCACTACAGAGTCCAAATGGTGGTGTATCGGCCTGGGAGCCAACTGGAGCTCCTAAATGGTTGGAGTGGCTCAACCCCGTCGAATTTCTTGAGGACCTTGTCATTGAATACGAATACATCGAGTGTACTTCATCTTCAATCCAGGCCCTAGTGTTGTTTAGGAAGTTGTACCCTGGTCACAGAAGGAAGGAGATTAACAACTTCATCACAAGGGCTGCAGACTACATTGAAAGTATCCAATACCCTGATGGTTCATGGTATGGAAACTGGGGTATCTGCTTTGTCTATGGAACATGGTTCGGAATCAAAGGATTAGAGGCTGCTGGAAGAACATACAACAACTGTGAGGCTGTTCGTAAAGGTGTCGATTTCTTGCTCAATACACAGAGGGAAGATGGTGGTTGGGGAGAGCACTACACCTCATGCACAAATAAGAAATACACAGCTCAGGACAGTACAAATCTGGTGCAAACTGCCCTTGGATTGATGGGTCTAATTCACGGTCGACAGGCTGAGAGAGATCCAACTCCTATCCACCGTGCCGCAAGGGTGTTGATGAATGGTCAATTGGATGACGGTGATTTCCCCCCAACAGGAACTGATGGGAGTTTTCATGAGGAATGCCATGTTGCACTATGCAGCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 ko01110 map00909 map01110
Pfam Domains
Protein Families
SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

711

Amino Acids

81.93

Weight (kDa)

5.44

Isoelectric Point (pI)

45.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQHop_cyclase_N PF13249 173 - 372 5.5e-22 Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C PF13243 392 - 691 7.5e-33 Squalene-hopene cyclase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66960 AT1G66960 AT1G66960 AT1G66960 AT1G78950 AT1G78950 AT1G78955 AT1G78955 AT1G78955 AT1G78955 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78970 AT1G78970 AT1G78970
fragaria_vesca FvH4_5g29480 FvH4_6g36450 FvH4_6g36450 FvH4_6g36500 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g37620
malus_domestica MD03G1089600.v1.1 MD09G1167700.v1.1 MD09G1168200.v1.1 MD09G1168300.v1.1 MD11G1098600.v1.1 MD17G1158300.v1.1 MD17G1158700.v1.1 MD17G1159500.v1.1 MD17G1160500.v1.1 MD17G1182500.v1.1 MD17G1182700.v1.1 MD17G1245800.v1.1 MD17G1246300.v1.1 MD17G1246600.v1.1
prunus_persica Prupe.3G025700_v2.0.a1 Prupe.3G025700_v2.0.a1 Prupe.3G025800_v2.0.a1 Prupe.3G025900_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026400_v2.0.a1 Prupe.3G026500_v2.0.a1 Prupe.3G026700_v2.0.a1 Prupe.3G026800_v2.0.a1
pyrus_communis pycom03g07120 pycom09g08520 pycom09g08590 pycom09g08610 pycom10g10070 pycom11g08310 pycom16g19880 pycom17g15190 pycom17g15300
rosa_chinensis RchiOBHm_Chr2g0136221 RchiOBHm_Chr2g0148451 RchiOBHm_Chr2g0148501 RchiOBHm_Chr2g0148521 RchiOBHm_Chr2g0148661 RchiOBHm_Chr2g0148701 RchiOBHm_Chr2g0148811 RchiOBHm_Chr2g0148941 RchiOBHm_Chr2g0148981 RchiOBHm_Chr2g0150451 RchiOBHm_Chr2g0150521 RchiOBHm_Chr2g0150611 RchiOBHm_Chr4g0420831 RchiOBHm_Chr5g0049791 RchiOBHm_Chr5g0049801
rosa_laevigata RLG00000007735 RLG00000020351 RLG00000020352 RLG00000020357 RLG00000020358 RLG00000020360 RLG00000029093 RLG00000030686
rosa_multiflora Rmu_co8274513.1_g000001 Rmu_sc0000048.1_g000003 Rmu_sc0000048.1_g000033 Rmu_sc0000048.1_g000039 Rmu_sc0001397.1_g000011 Rmu_sc0001998.1_g000039 Rmu_sc0002070.1_g000055 Rmu_sc0002082.1_g000052 Rmu_sc0003181.1_g000010 Rmu_sc0005104.1_g000005 Rmu_sc0006758.1_g000002 Rmu_sc0008411.1_g000029 Rmu_sc0008411.1_g000030 Rmu_sc0017472.1_g000001 Rmu_sc0017472.1_g000003 Rmu_sc0029876.1_g000001 Rmu_ssc0000480.1_g000024 Rmu_ssc0000480.1_g000036
rosa_roxburghii Rroxscaffold_2G00097070 Rroxscaffold_2G00098540 Rroxscaffold_2G00098560 Rroxscaffold_2G00098570 Rroxscaffold_2G00098650 Rroxscaffold_2G00098710 Rroxscaffold_2G00098740 Rroxscaffold_3G00225830 Rroxscaffold_3G00236870 Rroxscaffold_4G00312240
rosa_rugosa Rorug01G0047000 Rorug02G0372300 Rorug02G0407200 Rorug02G0407300 Rorug02G0408400 Rorug02G0421500 Rorug07G0208900
rosa_samantha Rh2AG380200 Rh2AG465700 Rh2AG465800 Rh2AG466800 Rh2AG467100 Rh2AG467600 Rh2AG480900 Rh2AG481400 Rh2AG481700 Rh2CG073500 Rh2CG452200 Rh2CG453200 Rh2CG453600 Rh2CG454100 Rh2CG467100 Rh2CG467800 Rh2CG468000 Rh2DG403100 Rh2DG487800 Rh2DG487900 Rh2DG488800 Rh2DG502000 Rh2DG502200 Rh2DG502300 Rh2DG503100 Rh3AG319300 Rh4AG227700 Rh4BG371800 Rh5AG327400 Rh5DG350600 Rh7AG349800
rosa_wichuraiana Rw0G010080 Rw2G031110 Rw2G037980 Rw2G037990 Rw2G038000 Rw2G038010 Rw2G038080 Rw2G038100 Rw2G039260 Rw2G039290 Rw2G039470 Rw4G019710 Rw5G030930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 26
AccB7I CCANNNNNTGG 1 cut(s) 1508
AccBSI CCGCTC 1 cut(s) 134
AccI GTMKAC 2 cut(s) 399, 1993
AccII CGCG 1 cut(s) 558
AciI CCGC 5 cut(s) 132, 556, 558, 602, 2031
AclWI GGATC 4 cut(s) 1070, 1083, 1362, 2003
AcsI RAATTY 5 cut(s) 142, 231, 990, 1229, 1571
AfaI GTAC 6 cut(s) 606, 885, 1242, 1615, 1658, 1945
AfeI AGCGCT 1 cut(s) 890
AfiI CCNNNNNNNGG 6 cut(s) 98, 509, 531, 543, 1508, 2081
AflII CTTAAG 1 cut(s) 1244
AgsI TTSAA 8 cut(s) 223, 949, 974, 1040, 1199, 1595, 1626, 1718
AjnI CCWGG 4 cut(s) 593, 1520, 1629, 1660
AjuI GAANNNNNNNTTGG 2 cut(s) 2039, 2071
AluBI AGCT 6 cut(s) 287, 1091, 1432, 1454, 1538, 1934
AluI AGCT 6 cut(s) 287, 1091, 1432, 1454, 1538, 1934
Alw21I GWGCWC 5 cut(s) 441, 568, 1189, 1540, 1902
Alw26I GTCTC 3 cut(s) 510, 781, 954
Alw44I GTGCAC 1 cut(s) 564
AlwI GGATC 4 cut(s) 1070, 1083, 1362, 2003
AlwNI CAGNNNCTG 1 cut(s) 2087
Aor51HI AGCGCT 1 cut(s) 890
AoxI GGCC 9 cut(s) 129, 279, 344, 499, 534, 757, 941, 1518, 1632
ApaI GGGCCC 1 cut(s) 538
ApaLI GTGCAC 1 cut(s) 564
ApeKI GCWGC 6 cut(s) 287, 302, 1401, 1703, 1808, 2127
ApoI RAATTY 5 cut(s) 142, 231, 990, 1229, 1571
AspLEI GCGC 3 cut(s) 459, 840, 891
AsuC2I CCSGG 2 cut(s) 408, 538
AsuHPI GGTGA 8 cut(s) 253, 269, 321, 970, 1148, 1416, 1451, 2078
AvaII GGWCC 4 cut(s) 362, 657, 1235, 1582
AxyI CCTNAGG 1 cut(s) 504
BaeGI GKGCMC 2 cut(s) 538, 568
BaeI ACNNNNGTAYC 2 cut(s) 1744, 1777
BamHI GGATCC 1 cut(s) 1075
BanI GGYRCC 1 cut(s) 26
BanII GRGCYC 2 cut(s) 538, 1540
BarI GAAGNNNNNNTAC 2 cut(s) 1606, 1638
BauI CACGAG 1 cut(s) 135
Bbv12I GWGCWC 5 cut(s) 441, 568, 1189, 1540, 1902
BbvCI CCTCAGC 1 cut(s) 1071
BbvI GCAGC 5 cut(s) 274, 289, 1388, 1690, 1795
BcgI CGANNNNNNTGC 2 cut(s) 77, 111
BciT130I CCWGG 4 cut(s) 595, 1522, 1631, 1662
BciVI GTATCC 2 cut(s) 404, 1733
BclI TGATCA 1 cut(s) 274
BcnI CCSGG 2 cut(s) 408, 538
BcoDI GTCTC 3 cut(s) 510, 781, 954
BfaI CTAG 3 cut(s) 1080, 1110, 1637
BfmI CTRYAG 4 cut(s) 706, 1383, 1493, 1704
BfoI RGCGCY 1 cut(s) 892
BfrI CTTAAG 1 cut(s) 1244
BfuI GTATCC 2 cut(s) 404, 1733
BisI GCNGC 8 cut(s) 132, 288, 303, 1402, 1704, 1809, 2031, 2128
BlpI GCTNAGC 1 cut(s) 435
BlsI GCNGC 8 cut(s) 133, 289, 304, 1403, 1705, 1810, 2032, 2129
Bme1390I CCNGG 6 cut(s) 408, 538, 595, 1522, 1631, 1662
Bme18I GGWCC 4 cut(s) 362, 657, 1235, 1582
BmiI GGNNCC 4 cut(s) 28, 536, 1077, 1527
BmrFI CCNGG 6 cut(s) 408, 538, 595, 1522, 1631, 1662
BmrI ACTGGG 1 cut(s) 1765
BmsI GCATC 7 cut(s) 85, 192, 1044, 1111, 1143, 1322, 1459
BmuI ACTGGG 1 cut(s) 1765
BpmI CTGGAG 1 cut(s) 1554
Bpu10I CCTNAGC 2 cut(s) 1071, 1935
Bpu1102I GCTNAGC 1 cut(s) 435
BpuEI CTTGAG 1 cut(s) 1598
BpuMI CCSGG 2 cut(s) 408, 538
BsaBI GATNNNNATC 1 cut(s) 1353
BsaJI CCNNGG 9 cut(s) 64, 120, 334, 384, 556, 874, 1521, 1660, 1966
BsaWI WCCGGW 2 cut(s) 106, 238
BsaXI ACNNNNNCTCC 2 cut(s) 1522, 1552
Bsc4I CCNNNNNNNGG 6 cut(s) 98, 509, 531, 543, 1508, 2081
Bse1I ACTGG 3 cut(s) 347, 1537, 1760
Bse21I CCTNAGG 1 cut(s) 504
Bse3DI GCAATG 2 cut(s) 1202, 1422
Bse8I GATNNNNATC 1 cut(s) 1353
BseBI CCWGG 4 cut(s) 595, 1522, 1631, 1662
BseDI CCNNGG 9 cut(s) 64, 120, 334, 384, 556, 874, 1521, 1660, 1966
BseGI GGATG 7 cut(s) 603, 682, 1037, 1285, 1337, 1367, 2065
BseJI GATNNNNATC 1 cut(s) 1353
BseLI CCNNNNNNNGG 6 cut(s) 98, 509, 531, 543, 1508, 2081
BseMI GCAATG 2 cut(s) 1202, 1422
BseMII CTCAG 8 cut(s) 342, 426, 495, 1062, 1289, 1446, 1949, 1992
BseNI ACTGG 3 cut(s) 347, 1537, 1760
BseRI GAGGAG 2 cut(s) 176, 759
BseSI GKGCMC 2 cut(s) 538, 568
BseXI GCAGC 5 cut(s) 274, 289, 1388, 1690, 1795
BseYI CCCAGC 2 cut(s) 173, 492
BsgI GTGCAG 1 cut(s) 1363
Bsh1236I CGCG 1 cut(s) 558
Bsh1285I CGRYCG 1 cut(s) 1993
BshFI GGCC 9 cut(s) 131, 281, 346, 501, 536, 759, 943, 1520, 1634
BshNI GGYRCC 1 cut(s) 26
BsiEI CGRYCG 1 cut(s) 1993
BsiHKAI GWGCWC 5 cut(s) 441, 568, 1189, 1540, 1902
BsiSI CCGG 5 cut(s) 107, 239, 408, 538, 1291
BslI CCNNNNNNNGG 6 cut(s) 98, 509, 531, 543, 1508, 2081
BsmAI GTCTC 3 cut(s) 510, 781, 954
BsmI GAATGC 3 cut(s) 322, 361, 2114
BsnI GGCC 9 cut(s) 131, 281, 346, 501, 536, 759, 943, 1520, 1634
Bsp120I GGGCCC 1 cut(s) 534
Bsp1286I GDGCHC 6 cut(s) 441, 538, 568, 1189, 1540, 1902
Bsp143I GATC 7 cut(s) 274, 814, 954, 1075, 1348, 1354, 2008
Bsp1720I GCTNAGC 1 cut(s) 435
Bsp19I CCATGG 3 cut(s) 64, 334, 874
BspACI CCGC 5 cut(s) 132, 556, 558, 602, 2031
BspANI GGCC 9 cut(s) 131, 281, 346, 501, 536, 759, 943, 1520, 1634
BspCNI CTCAG 8 cut(s) 343, 427, 496, 1063, 1288, 1447, 1948, 1993
BspFNI CGCG 1 cut(s) 558
BspHI TCATGA 2 cut(s) 390, 2101
BspLI GGNNCC 4 cut(s) 28, 536, 1077, 1527
BspMAI CTGCAG 2 cut(s) 1387, 1708
BspPI GGATC 4 cut(s) 1070, 1083, 1362, 2003
BspQI GCTCTTC 1 cut(s) 105
BspT107I GGYRCC 1 cut(s) 26
BspTI CTTAAG 1 cut(s) 1244
BsrBI CCGCTC 1 cut(s) 134
BsrDI GCAATG 2 cut(s) 1202, 1422
BsrI ACTGG 3 cut(s) 347, 1537, 1760
BssECI CCNNGG 9 cut(s) 64, 120, 334, 384, 556, 874, 1521, 1660, 1966
BssMI GATC 7 cut(s) 274, 814, 954, 1075, 1348, 1354, 2008
BssNAI GTATAC 1 cut(s) 400
BssSI CACGAG 1 cut(s) 135
BssT1I CCWWGG 5 cut(s) 64, 334, 384, 874, 1966
Bst1107I GTATAC 1 cut(s) 400
Bst2BI CACGAG 1 cut(s) 135
Bst2UI CCWGG 4 cut(s) 595, 1522, 1631, 1662
Bst4CI ACNGT 7 cut(s) 707, 1013, 1832, 1943, 1991, 2027, 2066
Bst6I CTCTTC 3 cut(s) 105, 874, 933
BstAFI CTTAAG 1 cut(s) 1244
BstC8I GCNNGC 4 cut(s) 100, 117, 348, 1059
BstDEI CTNAG 9 cut(s) 206, 351, 435, 504, 1071, 1275, 1455, 1935, 2001
BstDSI CCRYGG 4 cut(s) 64, 334, 556, 874
BstENI CCTNNNNNAGG 1 cut(s) 529
BstF5I GGATG 7 cut(s) 603, 682, 1037, 1285, 1337, 1367, 2065
BstFNI CGCG 1 cut(s) 558
BstH2I RGCGCY 1 cut(s) 892
BstHHI GCGC 3 cut(s) 459, 840, 891
BstKTI GATC 7 cut(s) 277, 817, 957, 1078, 1351, 1357, 2011
BstMAI GTCTC 3 cut(s) 510, 781, 954
BstMBI GATC 7 cut(s) 274, 814, 954, 1075, 1348, 1354, 2008
BstMCI CGRYCG 1 cut(s) 1993
BstMWI GCNNNNNNNGC 4 cut(s) 287, 356, 498, 1067
BstNI CCWGG 4 cut(s) 595, 1522, 1631, 1662
BstSCI CCNGG 6 cut(s) 406, 536, 593, 1520, 1629, 1660
BstSFI CTRYAG 4 cut(s) 706, 1383, 1493, 1704
BstSLI GKGCMC 2 cut(s) 538, 568
BstUI CGCG 1 cut(s) 558
BstV1I GCAGC 5 cut(s) 274, 289, 1388, 1690, 1795
BstX2I RGATCY 2 cut(s) 1075, 2008
BstXI CCANNNNNNTGG 1 cut(s) 654
BstYI RGATCY 2 cut(s) 1075, 2008
BstZ17I GTATAC 1 cut(s) 400
Bsu36I CCTNAGG 1 cut(s) 504
BsuI GTATCC 2 cut(s) 404, 1733
BsuRI GGCC 9 cut(s) 131, 281, 346, 501, 536, 759, 943, 1520, 1634
BtgI CCRYGG 4 cut(s) 64, 334, 556, 874
BtsCI GGATG 7 cut(s) 603, 682, 1037, 1285, 1337, 1367, 2065
BtsI GCAGTG 2 cut(s) 184, 1380
BtsIMutI CAGTG 3 cut(s) 184, 340, 1380
Cac8I GCNNGC 4 cut(s) 100, 117, 348, 1059
CaiI CAGNNNCTG 1 cut(s) 2087
CciI TCATGA 2 cut(s) 390, 2101
CfoI GCGC 3 cut(s) 459, 840, 891
Cfr42I CCGCGG 1 cut(s) 559
Csp6I GTAC 6 cut(s) 605, 884, 1241, 1614, 1657, 1944
CspCI CAANNNNNGTGG 2 cut(s) 806, 841
CviQI GTAC 6 cut(s) 605, 884, 1241, 1614, 1657, 1944
DdeI CTNAG 9 cut(s) 206, 351, 435, 504, 1071, 1275, 1455, 1935, 2001
DpnI GATC 7 cut(s) 276, 816, 956, 1077, 1350, 1356, 2010
DpnII GATC 7 cut(s) 274, 814, 954, 1075, 1348, 1354, 2008
Eam1104I CTCTTC 3 cut(s) 105, 874, 933
EarI CTCTTC 3 cut(s) 105, 874, 933
Ecl136II GAGCTC 1 cut(s) 1538
Eco130I CCWWGG 5 cut(s) 64, 334, 384, 874, 1966
Eco24I GRGCYC 2 cut(s) 538, 1540
Eco47I GGWCC 4 cut(s) 362, 657, 1235, 1582
Eco47III AGCGCT 1 cut(s) 890
Eco53kI GAGCTC 1 cut(s) 1538
Eco81I CCTNAGG 1 cut(s) 504
EcoICRI GAGCTC 1 cut(s) 1538
EcoNI CCTNNNNNAGG 1 cut(s) 529
EcoO109I RGGNCCY 4 cut(s) 500, 534, 1582, 1633
EcoRII CCWGG 4 cut(s) 593, 1520, 1629, 1660
EcoT14I CCWWGG 5 cut(s) 64, 334, 384, 874, 1966
EcoT38I GRGCYC 2 cut(s) 538, 1540
ErhI CCWWGG 5 cut(s) 64, 334, 384, 874, 1966
FalI AAGNNNNNCTT 4 cut(s) 437, 469, 1191, 1223
FbaI TGATCA 1 cut(s) 274
FblI GTMKAC 2 cut(s) 399, 1993
Fnu4HI GCNGC 8 cut(s) 132, 288, 303, 1402, 1704, 1809, 2031, 2128
FokI GGATG 7 cut(s) 610, 669, 1044, 1292, 1344, 1374, 2072
FriOI GRGCYC 2 cut(s) 538, 1540
Fsp4HI GCNGC 8 cut(s) 132, 288, 303, 1402, 1704, 1809, 2031, 2128
FspBI CTAG 3 cut(s) 1080, 1110, 1637
GlaI GCGC 3 cut(s) 458, 839, 890
GluI GCNGC 8 cut(s) 132, 288, 303, 1402, 1704, 1809, 2031, 2128
GsaI CCCAGC 2 cut(s) 177, 496
GsuI CTGGAG 1 cut(s) 1554
HaeII RGCGCY 1 cut(s) 892
HaeIII GGCC 9 cut(s) 131, 281, 346, 501, 536, 759, 943, 1520, 1634
HapII CCGG 5 cut(s) 107, 239, 408, 538, 1291
HhaI GCGC 3 cut(s) 459, 840, 891
Hin6I GCGC 3 cut(s) 457, 838, 889
HinP1I GCGC 3 cut(s) 457, 838, 889
HincII GTYRAC 2 cut(s) 714, 1994
HindII GTYRAC 2 cut(s) 714, 1994
HindIII AAGCTT 1 cut(s) 1089
HinfI GANTC 6 cut(s) 547, 829, 1195, 1216, 1498, 1791
HpaII CCGG 5 cut(s) 107, 239, 408, 538, 1291
HphI GGTGA 8 cut(s) 253, 269, 321, 970, 1148, 1416, 1451, 2078
Hpy166II GTNNAC 5 cut(s) 400, 566, 714, 1614, 1994
Hpy188I TCNGA 7 cut(s) 20, 201, 477, 959, 1348, 1375, 1790
Hpy8I GTNNAC 5 cut(s) 400, 566, 714, 1614, 1994
Hpy99I CGWCG 1 cut(s) 1571
HpyAV CCTTC 8 cut(s) 248, 319, 439, 491, 620, 955, 1665, 1669
HpyCH4III ACNGT 7 cut(s) 707, 1013, 1832, 1943, 1991, 2027, 2066
HpyF10VI GCNNNNNNNGC 4 cut(s) 287, 356, 498, 1067
HpyF3I CTNAG 9 cut(s) 206, 351, 435, 504, 1071, 1275, 1455, 1935, 2001
HspAI GCGC 3 cut(s) 457, 838, 889
Ksp22I TGATCA 1 cut(s) 274
KspI CCGCGG 1 cut(s) 559
Kzo9I GATC 7 cut(s) 274, 814, 954, 1075, 1348, 1354, 2008
LguI GCTCTTC 1 cut(s) 105
LmnI GCTCC 4 cut(s) 175, 1525, 1535, 1543
Lsp1109I GCAGC 5 cut(s) 274, 289, 1388, 1690, 1795
LweI GCATC 7 cut(s) 85, 192, 1044, 1111, 1143, 1322, 1459
MaeI CTAG 3 cut(s) 1080, 1110, 1637
MaeIII GTNAC 7 cut(s) 179, 327, 338, 1114, 1422, 1488, 1664
MalI GATC 7 cut(s) 276, 816, 956, 1077, 1350, 1356, 2010
MbiI CCGCTC 1 cut(s) 134
MboI GATC 7 cut(s) 274, 814, 954, 1075, 1348, 1354, 2008
MfeI CAATTG 2 cut(s) 782, 2054
MflI RGATCY 2 cut(s) 1075, 2008
MhlI GDGCHC 6 cut(s) 441, 538, 568, 1189, 1540, 1902
MlyI GAGTC 2 cut(s) 838, 1507
MmeI TCCRAC 2 cut(s) 1530, 2036
MseI TTAA 4 cut(s) 12, 1095, 1245, 1683
MslI CAYNNNNRTG 2 cut(s) 846, 1396
MspA1I CMGCKG 2 cut(s) 287, 558
MspCI CTTAAG 1 cut(s) 1244
MspI CCGG 5 cut(s) 107, 239, 408, 538, 1291
MspR9I CCNGG 6 cut(s) 408, 538, 595, 1522, 1631, 1662
MunI CAATTG 2 cut(s) 782, 2054
Mva1269I GAATGC 3 cut(s) 322, 361, 2114
MvaI CCWGG 4 cut(s) 595, 1522, 1631, 1662
MvnI CGCG 1 cut(s) 558
MwoI GCNNNNNNNGC 4 cut(s) 287, 356, 498, 1067
NciI CCSGG 2 cut(s) 408, 538
NcoI CCATGG 3 cut(s) 64, 334, 874
NdeII GATC 7 cut(s) 274, 814, 954, 1075, 1348, 1354, 2008
NlaIV GGNNCC 4 cut(s) 28, 536, 1077, 1527
NmeAIII GCCGAG 1 cut(s) 145
NmuCI GTSAC 6 cut(s) 179, 327, 338, 1422, 1488, 1664
PagI TCATGA 2 cut(s) 390, 2101
PciSI GCTCTTC 1 cut(s) 105
PcsI WCGNNNNNNNCGW 1 cut(s) 1605
PctI GAATGC 3 cut(s) 322, 361, 2114
PfeI GAWTC 4 cut(s) 547, 1195, 1216, 1791
PflFI GACNNNGTC 1 cut(s) 1586
PflMI CCANNNNNTGG 1 cut(s) 1508
PkrI GCNGC 8 cut(s) 133, 289, 304, 1403, 1705, 1810, 2032, 2129
PleI GAGTC 2 cut(s) 837, 1506
PpsI GAGTC 2 cut(s) 837, 1506
PpuMI RGGWCCY 1 cut(s) 1582
Psp124BI GAGCTC 1 cut(s) 1540
Psp5II RGGWCCY 1 cut(s) 1582
Psp6I CCWGG 4 cut(s) 593, 1520, 1629, 1660
PspFI CCCAGC 2 cut(s) 173, 492
PspGI CCWGG 4 cut(s) 593, 1520, 1629, 1660
PspN4I GGNNCC 4 cut(s) 28, 536, 1077, 1527
PspOMI GGGCCC 1 cut(s) 534
PspPPI RGGWCCY 1 cut(s) 1582
PsrI GAACNNNNNNTAC 2 cut(s) 1001, 1033
PstI CTGCAG 2 cut(s) 1387, 1708
PstNI CAGNNNCTG 1 cut(s) 2087
PsuI RGATCY 2 cut(s) 1075, 2008
PsyI GACNNNGTC 1 cut(s) 1586
PvuII CAGCTG 1 cut(s) 287
RsaI GTAC 6 cut(s) 606, 885, 1242, 1615, 1658, 1945
RsaNI GTAC 6 cut(s) 605, 884, 1241, 1614, 1657, 1944
RseI CAYNNNNRTG 2 cut(s) 846, 1396
SacI GAGCTC 1 cut(s) 1540
SacII CCGCGG 1 cut(s) 559
SalI GTCGAC 1 cut(s) 1992
SapI GCTCTTC 1 cut(s) 105
SaqAI TTAA 4 cut(s) 12, 1095, 1245, 1683
SatI GCNGC 8 cut(s) 132, 288, 303, 1402, 1704, 1809, 2031, 2128
Sau3AI GATC 7 cut(s) 274, 814, 954, 1075, 1348, 1354, 2008
SchI GAGTC 2 cut(s) 838, 1507
ScrFI CCNGG 6 cut(s) 408, 538, 595, 1522, 1631, 1662
SduI GDGCHC 6 cut(s) 441, 538, 568, 1189, 1540, 1902
SfaNI GCATC 7 cut(s) 85, 192, 1044, 1111, 1143, 1322, 1459
SfcI CTRYAG 4 cut(s) 706, 1383, 1493, 1704
Sfr303I CCGCGG 1 cut(s) 559
SgrBI CCGCGG 1 cut(s) 559
SinI GGWCC 4 cut(s) 362, 657, 1235, 1582
SmiMI CAYNNNNRTG 2 cut(s) 846, 1396
SmlI CTYRAG 2 cut(s) 1244, 1577
SmoI CTYRAG 2 cut(s) 1244, 1577
SsiI CCGC 5 cut(s) 132, 556, 558, 602, 2031
SspI AATATT 2 cut(s) 572, 916
SspMI CTAG 3 cut(s) 1080, 1110, 1637
SstI GAGCTC 1 cut(s) 1540
StyD4I CCNGG 6 cut(s) 406, 536, 593, 1520, 1629, 1660
StyI CCWWGG 5 cut(s) 64, 334, 384, 874, 1966
TaaI ACNGT 7 cut(s) 707, 1013, 1832, 1943, 1991, 2027, 2066
TaqI TCGA 6 cut(s) 87, 514, 1569, 1608, 1854, 1993
TatI WGTACW 4 cut(s) 883, 1240, 1613, 1943
TauI GCSGC 2 cut(s) 134, 2033
TfiI GAWTC 4 cut(s) 547, 1195, 1216, 1791
Tru1I TTAA 4 cut(s) 12, 1095, 1245, 1683
Tru9I TTAA 4 cut(s) 12, 1095, 1245, 1683
TscAI CASTG 3 cut(s) 184, 347, 1387
TseFI GTSAC 6 cut(s) 179, 327, 338, 1422, 1488, 1664
TseI GCWGC 6 cut(s) 287, 302, 1401, 1703, 1808, 2127
Tsp45I GTSAC 6 cut(s) 179, 327, 338, 1422, 1488, 1664
TspDTI ATGAA 9 cut(s) 671, 983, 1242, 1296, 1608, 1681, 1731, 2060, 2090
TspGWI ACGGA 1 cut(s) 1304
TspRI CASTG 3 cut(s) 184, 347, 1387
Tth111I GACNNNGTC 1 cut(s) 1586
Van91I CCANNNNNTGG 1 cut(s) 1508
Vha464I CTTAAG 1 cut(s) 1244
VneI GTGCAC 1 cut(s) 564
VpaK11BI GGWCC 4 cut(s) 362, 657, 1235, 1582
XagI CCTNNNNNAGG 1 cut(s) 529
XapI RAATTY 5 cut(s) 142, 231, 990, 1229, 1571
XcmI CCANNNNNNNNNTGG 1 cut(s) 1733
XmiI GTMKAC 2 cut(s) 399, 1993
XspI CTAG 3 cut(s) 1080, 1110, 1637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.