Rh5DG350600

Belongs to the terpene cyclase mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000173
Physical Location & Seq
Reverse (-)
48285338 .. 48289302
3965 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG350600.1

Sequence Viewer

Length: 2292 bp
ATGTGGAGAATTGAGTATGGAAAGGGTGCAAATGACCCTCATTTATTCAGCACAAACAACTTGCATGGGAGGCAGACATGGAAGTTTGACCCAAATGCAGGTACACCAGAAGAGCGAGCAGAGGTTGAAGCCGCTCGCGAGAATTACTACCAAAATCGGTTCAAGATCCAGCCCAGTAGTGATCTCCTTTGGCGTTTTCAGATGCTAAGAGAGAGAAACTTCAAGCAAGAAATTCCTCCAGTAAGAATTGGGGAAGGTGATGACATTACAGTTTATCAGGCCACAGCTGCCTATAGGAGGGCTGCAACCTTCTGGAATGCCTTGCAATCACCCCATGGTCATTGGCCTGCTGAAAATTCTGGCATAAATTTTTTCTGTTCTCCCTTGGTAATGACTTTATACTCAATGGGATATCTTAATGTTGTTTTCTCTGCTGAGCATAAGAAGGAAATCTTGCGCTACTCGTACAATCATCAGAATGAAGATGGTGGGTGGGGATTGCACATAGCAGGGCCTAGTATGATGTTCACCACATGTCTCAACTACTGTATGATGCGTATCCTCGGAGAAGGCACTGAGGGCGGTCGAGATAATAACTGTGCAAGGGCCCGGAAATGGATTCTTGACCGCGGTGGTGCACAATATTCTGCTTCATGGGGAAAGACCTGGATGGCGATACTTGGTGTTTATGATTGGGAAGGAAGCAACCCTATACCCCCGGAGTTTTGGACTTACCCCACTCTGCTTCCTTTTCATCCATCAAAGATGTTGTGCTACTGTAGGTTAACTTACCTACCCATGTCCTACTTTTATGCAAAAAAATTTGTTGGCCCAATCACTCCTCTGGTTGAACAATTGAGACAAGAAATTTACTGTGAACCTTACAGTGAAATTAAGTGGAGTAAAGTGCGACATCATTGTGCAAAGGAAGATAACTACTATCCTCATGGAAGGGTACAACGTTTTATGTGGGACACTCTTTACCATGTTTGTGAGCCTATTCTTGCTTGGTGGCCCTTTAAGAAGATCAGAGACAATGCTATTCAATTTACCATTGACCAAATCCATTATGAAGATGAGAACAGTCGATACATTACCATTGGATGTATCGAAAAGCCATTAATGATGCTTGCCTGTTGGGTTGAGGATCCTAGCGGAGAAGCTTTTAAGAAGCATCTTCCTAGACTTGATGATTATATTTGGGTCGGTGAAGATGGAATCAAGATTCAGAGTTTTGGTAGCCAGACTTGGGATTGTTCTCTTACAATCCAAGCTTTGCTTGCTGGGAATCTCCATAATGAATTTGGACCAGTACTTAAGAAAGCACACGACTTCCTAAAGATATCCCAGGTGAGGATCAATCCTTGTGGTGACTACCTAGCTCATTTCCGTCACATTTCCAAAGGATGCTGGACTTTCTCTGATCGGGATCATGGATGGCAAGTTTCAGATTGCACTGCAGAAGCATTGAGGTGCTGCTGTCTGTTTGCAATGATGTCACCAGAGGTTGTTGGCGAGCCAATGGAAGCCGAGTGTATGTATGATGCTGTCAATGTCATAATGTCACTACAGAGTCCAAATGGTGGTGTATCAGCGTGGGAGCCGACTGGAGCTCCTAAATGGTTGGAGTGGCTCAACCCTGTTGAATTTTTTGAGGACCTTGTCATTGAATATGATTACGTCGAGTGCACTTCATCTTCGATTCAGGCCTTACTTTTGTTTAGGAAGTTGTATCCTAATCACAGAAGGAAAGAGATCAACAACTTCATCACAAGGGCTGCAGACTACATTGAAAGCATACAATACGCTGATGGCTCATGGTATGGAAACTGGGGTATCTGCTTTCTCTATGGAACATGGTTTGCAATCAAAGGGTTAGAGGCAGCAGGAAGAACCTACCACAATTGCGAGGCAGTTCGCAAAGGTGTTGACTTTTTGCTAAAAACACAAAGGGAAGATGGTGGTTGGGGAGAGCACTACACCTCATGCACGAACAAGAAATACACAGCTCAAGACAGTACAAATCTGGTGCAAACTGCCCTCGGATTAATGGGTCTAATTCACGGTCGACAGGGTGAGAGAGATCCAACTCCTATTCACCGTGCTGCAAGGGTCTTGATGAACGGTCAGTTGGATGATGGTGATTTTCCCCAACAGGAACTGGTGGGAGTTTTCATGAGGAATGCAATGTTACACTTTGGAGGATATAGGAATGTTTTCCCATTGTGGGCTCTTGGAGAGTATCGTACGTACTCTGGAGCAGTTGCTTTACCAAAAGGATTTGAACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 ko01110 map00909 map01110
Pfam Domains
Protein Families
SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

763

Amino Acids

88.02

Weight (kDa)

6.16

Isoelectric Point (pI)

40.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQHop_cyclase_N PF13249 100 - 401 1.9e-33 Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C PF13243 414 - 749 2.4e-47 Squalene-hopene cyclase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66960 AT1G66960 AT1G66960 AT1G66960 AT1G78950 AT1G78950 AT1G78955 AT1G78955 AT1G78955 AT1G78955 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78970 AT1G78970 AT1G78970
fragaria_vesca FvH4_5g29480 FvH4_6g36450 FvH4_6g36450 FvH4_6g36500 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g37620
malus_domestica MD03G1089600.v1.1 MD09G1167700.v1.1 MD09G1168200.v1.1 MD09G1168300.v1.1 MD11G1098600.v1.1 MD17G1158300.v1.1 MD17G1158700.v1.1 MD17G1159500.v1.1 MD17G1160500.v1.1 MD17G1182500.v1.1 MD17G1182700.v1.1 MD17G1245800.v1.1 MD17G1246300.v1.1 MD17G1246600.v1.1
prunus_persica Prupe.3G025700_v2.0.a1 Prupe.3G025700_v2.0.a1 Prupe.3G025800_v2.0.a1 Prupe.3G025900_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026400_v2.0.a1 Prupe.3G026500_v2.0.a1 Prupe.3G026700_v2.0.a1 Prupe.3G026800_v2.0.a1
pyrus_communis pycom03g07120 pycom09g08520 pycom09g08590 pycom09g08610 pycom10g10070 pycom11g08310 pycom16g19880 pycom17g15190 pycom17g15300
rosa_chinensis RchiOBHm_Chr2g0136221 RchiOBHm_Chr2g0148451 RchiOBHm_Chr2g0148501 RchiOBHm_Chr2g0148521 RchiOBHm_Chr2g0148661 RchiOBHm_Chr2g0148701 RchiOBHm_Chr2g0148811 RchiOBHm_Chr2g0148941 RchiOBHm_Chr2g0148981 RchiOBHm_Chr2g0150451 RchiOBHm_Chr2g0150521 RchiOBHm_Chr2g0150611 RchiOBHm_Chr4g0420831 RchiOBHm_Chr5g0049791 RchiOBHm_Chr5g0049801
rosa_laevigata RLG00000007735 RLG00000020351 RLG00000020352 RLG00000020357 RLG00000020358 RLG00000020360 RLG00000029093 RLG00000030686
rosa_multiflora Rmu_co8274513.1_g000001 Rmu_sc0000048.1_g000003 Rmu_sc0000048.1_g000033 Rmu_sc0000048.1_g000039 Rmu_sc0001397.1_g000011 Rmu_sc0001998.1_g000039 Rmu_sc0002070.1_g000055 Rmu_sc0002082.1_g000052 Rmu_sc0003181.1_g000010 Rmu_sc0005104.1_g000005 Rmu_sc0006758.1_g000002 Rmu_sc0008411.1_g000029 Rmu_sc0008411.1_g000030 Rmu_sc0017472.1_g000001 Rmu_sc0017472.1_g000003 Rmu_sc0029876.1_g000001 Rmu_ssc0000480.1_g000024 Rmu_ssc0000480.1_g000036
rosa_roxburghii Rroxscaffold_2G00097070 Rroxscaffold_2G00098540 Rroxscaffold_2G00098560 Rroxscaffold_2G00098570 Rroxscaffold_2G00098650 Rroxscaffold_2G00098710 Rroxscaffold_2G00098740 Rroxscaffold_3G00225830 Rroxscaffold_3G00236870 Rroxscaffold_4G00312240
rosa_rugosa Rorug01G0047000 Rorug02G0372300 Rorug02G0407200 Rorug02G0407300 Rorug02G0408400 Rorug02G0421500 Rorug07G0208900
rosa_samantha Rh2AG380200 Rh2AG465700 Rh2AG465800 Rh2AG466800 Rh2AG467100 Rh2AG467600 Rh2AG480900 Rh2AG481400 Rh2AG481700 Rh2CG073500 Rh2CG452200 Rh2CG453200 Rh2CG453600 Rh2CG454100 Rh2CG467100 Rh2CG467800 Rh2CG468000 Rh2DG403100 Rh2DG487800 Rh2DG487900 Rh2DG488800 Rh2DG502000 Rh2DG502200 Rh2DG502300 Rh2DG503100 Rh3AG319300 Rh4AG227700 Rh4BG371800 Rh5AG327400 Rh5DG350600 Rh7AG349800
rosa_wichuraiana Rw0G010080 Rw2G031110 Rw2G037980 Rw2G037990 Rw2G038000 Rw2G038010 Rw2G038080 Rw2G038100 Rw2G039260 Rw2G039290 Rw2G039470 Rw4G019710 Rw5G030930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 89
AccB7I CCANNNNNTGG 1 cut(s) 1583
AccBSI CCGCTC 1 cut(s) 134
AccI GTMKAC 1 cut(s) 2068
AccII CGCG 2 cut(s) 138, 630
AciI CCGC 5 cut(s) 132, 582, 628, 630, 1155
AclI AACGTT 1 cut(s) 961
AclWI GGATC 6 cut(s) 160, 1142, 1155, 1364, 1437, 2078
AcsI RAATTY 7 cut(s) 231, 355, 367, 821, 867, 1301, 1646
AfaI GTAC 7 cut(s) 103, 467, 957, 1314, 2020, 2248, 2252
AfiI CCNNNNNNNGG 7 cut(s) 98, 297, 615, 1249, 1353, 1583, 2227
AflII CTTAAG 1 cut(s) 1316
AflIII ACRYGT 1 cut(s) 533
AgsI TTSAA 9 cut(s) 128, 163, 223, 851, 1046, 1646, 1670, 1793, 2285
AjnI CCWGG 2 cut(s) 665, 1347
AjuI GAANNNNNNNTTGG 2 cut(s) 2114, 2146
AluBI AGCT 6 cut(s) 287, 1163, 1274, 1382, 1613, 2009
AluI AGCT 6 cut(s) 287, 1163, 1274, 1382, 1613, 2009
Alw21I GWGCWC 4 cut(s) 640, 1615, 1691, 1977
Alw26I GTCTC 3 cut(s) 542, 853, 1026
Alw44I GTGCAC 2 cut(s) 636, 1687
AlwI GGATC 6 cut(s) 160, 1142, 1155, 1364, 1437, 2078
AlwNI CAGNNNCTG 1 cut(s) 2161
AoxI GGCC 7 cut(s) 279, 344, 512, 606, 829, 1013, 1707
ApaI GGGCCC 1 cut(s) 610
ApaLI GTGCAC 2 cut(s) 636, 1687
ApeKI GCWGC 6 cut(s) 287, 302, 1476, 1778, 1883, 2105
ApoI RAATTY 7 cut(s) 231, 355, 367, 821, 867, 1301, 1646
AseI ATTAAT 2 cut(s) 1121, 2048
Asp700I GAANNNNTTC 1 cut(s) 2216
AspLEI GCGC 1 cut(s) 459
AspS9I GGNCC 7 cut(s) 512, 606, 607, 830, 1014, 1307, 1657
AsuC2I CCSGG 2 cut(s) 610, 719
AvaII GGWCC 2 cut(s) 1307, 1657
BaeGI GKGCMC 3 cut(s) 610, 640, 1691
BaeI ACNNNNGTAYC 2 cut(s) 1819, 1852
BamHI GGATCC 1 cut(s) 1147
BanII GRGCYC 3 cut(s) 610, 1615, 2233
Bbv12I GWGCWC 4 cut(s) 640, 1615, 1691, 1977
BbvI GCAGC 6 cut(s) 274, 289, 1463, 1765, 1895, 2092
BccI CCATC 8 cut(s) 479, 664, 766, 1208, 1431, 1805, 1952, 2132
BciT130I CCWGG 2 cut(s) 667, 1349
BciVI GTATCC 2 cut(s) 569, 1743
BcnI CCSGG 2 cut(s) 610, 719
BcoDI GTCTC 3 cut(s) 542, 853, 1026
BfaI CTAG 4 cut(s) 516, 1152, 1182, 1379
BfmI CTRYAG 5 cut(s) 292, 778, 1458, 1568, 1779
BfrI CTTAAG 1 cut(s) 1316
BfuAI ACCTGC 1 cut(s) 89
BfuI GTATCC 2 cut(s) 569, 1743
BisI GCNGC 7 cut(s) 132, 288, 303, 1477, 1779, 1884, 2106
BlpI GCTNAGC 1 cut(s) 435
BlsI GCNGC 7 cut(s) 133, 289, 304, 1478, 1780, 1885, 2107
BmcAI AGTACT 1 cut(s) 1314
Bme1390I CCNGG 4 cut(s) 610, 667, 719, 1349
Bme18I GGWCC 2 cut(s) 1307, 1657
BmgT120I GGNCC 7 cut(s) 512, 606, 607, 830, 1014, 1307, 1657
BmiI GGNNCC 3 cut(s) 608, 1149, 1602
BmrFI CCNGG 4 cut(s) 610, 667, 719, 1349
BmrI ACTGGG 2 cut(s) 168, 1840
BmsI GCATC 6 cut(s) 192, 543, 1116, 1183, 1397, 1534
BmuI ACTGGG 2 cut(s) 168, 1840
BpmI CTGGAG 3 cut(s) 222, 1629, 2277
Bpu1102I GCTNAGC 1 cut(s) 435
BpuEI CTTGAG 1 cut(s) 1995
BpuMI CCSGG 2 cut(s) 610, 719
BsaAI YACGTR 1 cut(s) 2250
BsaBI GATNNNNATC 2 cut(s) 557, 1428
BsaJI CCNNGG 7 cut(s) 334, 384, 562, 628, 717, 1347, 2041
BsaXI ACNNNNNCTCC 2 cut(s) 1597, 1627
Bsc4I CCNNNNNNNGG 7 cut(s) 98, 297, 615, 1249, 1353, 1583, 2227
Bse1I ACTGG 6 cut(s) 174, 239, 1310, 1612, 1835, 2166
Bse3DI GCAATG 2 cut(s) 1497, 2193
Bse8I GATNNNNATC 2 cut(s) 557, 1428
BseBI CCWGG 2 cut(s) 667, 1349
BseDI CCNNGG 7 cut(s) 334, 384, 562, 628, 717, 1347, 2041
BseGI GGATG 6 cut(s) 675, 754, 1109, 1412, 1442, 2140
BseJI GATNNNNATC 2 cut(s) 557, 1428
BseLI CCNNNNNNNGG 7 cut(s) 98, 297, 615, 1249, 1353, 1583, 2227
BseMI GCAATG 2 cut(s) 1497, 2193
BseMII CTCAG 2 cut(s) 426, 567
BseNI ACTGG 6 cut(s) 174, 239, 1310, 1612, 1835, 2166
BseRI GAGGAG 1 cut(s) 831
BseSI GKGCMC 3 cut(s) 610, 640, 1691
BseXI GCAGC 6 cut(s) 274, 289, 1463, 1765, 1895, 2092
BseYI CCCAGC 1 cut(s) 1283
Bsh1236I CGCG 2 cut(s) 138, 630
Bsh1285I CGRYCG 2 cut(s) 586, 2068
BshFI GGCC 7 cut(s) 281, 346, 514, 608, 831, 1015, 1709
BsiEI CGRYCG 2 cut(s) 586, 2068
BsiHKAI GWGCWC 4 cut(s) 640, 1615, 1691, 1977
BsiSI CCGG 2 cut(s) 610, 719
BsiWI CGTACG 1 cut(s) 2246
BslFI GGGAC 1 cut(s) 986
BslI CCNNNNNNNGG 7 cut(s) 98, 297, 615, 1249, 1353, 1583, 2227
BsmAI GTCTC 3 cut(s) 542, 853, 1026
BsmFI GGGAC 1 cut(s) 986
BsmI GAATGC 2 cut(s) 322, 2188
BsnI GGCC 7 cut(s) 281, 346, 514, 608, 831, 1015, 1709
Bsp120I GGGCCC 1 cut(s) 606
Bsp1286I GDGCHC 6 cut(s) 610, 640, 1615, 1691, 1977, 2233
Bsp143I GATC 9 cut(s) 165, 181, 1026, 1147, 1356, 1423, 1429, 1755, 2083
Bsp1720I GCTNAGC 1 cut(s) 435
Bsp19I CCATGG 1 cut(s) 334
Bsp68I TCGCGA 1 cut(s) 138
BspACI CCGC 5 cut(s) 132, 582, 628, 630, 1155
BspANI GGCC 7 cut(s) 281, 346, 514, 608, 831, 1015, 1709
BspCNI CTCAG 2 cut(s) 427, 568
BspFNI CGCG 2 cut(s) 138, 630
BspHI TCATGA 1 cut(s) 2175
BspLI GGNNCC 3 cut(s) 608, 1149, 1602
BspMAI CTGCAG 2 cut(s) 1462, 1783
BspMI ACCTGC 1 cut(s) 89
BspPI GGATC 6 cut(s) 160, 1142, 1155, 1364, 1437, 2078
BspQI GCTCTTC 1 cut(s) 105
BspTI CTTAAG 1 cut(s) 1316
BsrBI CCGCTC 1 cut(s) 134
BsrDI GCAATG 2 cut(s) 1497, 2193
BsrI ACTGG 6 cut(s) 174, 239, 1310, 1612, 1835, 2166
BssECI CCNNGG 7 cut(s) 334, 384, 562, 628, 717, 1347, 2041
BssMI GATC 9 cut(s) 165, 181, 1026, 1147, 1356, 1423, 1429, 1755, 2083
BssT1I CCWWGG 2 cut(s) 334, 384
Bst2UI CCWGG 2 cut(s) 667, 1349
Bst6I CTCTTC 1 cut(s) 105
BstAFI CTTAAG 1 cut(s) 1316
BstBAI YACGTR 1 cut(s) 2250
BstC8I GCNNGC 6 cut(s) 117, 136, 348, 1131, 1281, 1517
BstDEI CTNAG 3 cut(s) 206, 435, 576
BstDSI CCRYGG 2 cut(s) 334, 628
BstENI CCTNNNNNAGG 1 cut(s) 295
BstF5I GGATG 6 cut(s) 675, 754, 1109, 1412, 1442, 2140
BstFNI CGCG 2 cut(s) 138, 630
BstHHI GCGC 1 cut(s) 459
BstKTI GATC 9 cut(s) 168, 184, 1029, 1150, 1359, 1426, 1432, 1758, 2086
BstMAI GTCTC 3 cut(s) 542, 853, 1026
BstMBI GATC 9 cut(s) 165, 181, 1026, 1147, 1356, 1423, 1429, 1755, 2083
BstMCI CGRYCG 2 cut(s) 586, 2068
BstMWI GCNNNNNNNGC 4 cut(s) 70, 287, 579, 1280
BstNI CCWGG 2 cut(s) 667, 1349
BstNSI RCATGY 1 cut(s) 537
BstSCI CCNGG 4 cut(s) 608, 665, 717, 1347
BstSFI CTRYAG 5 cut(s) 292, 778, 1458, 1568, 1779
BstSLI GKGCMC 3 cut(s) 610, 640, 1691
BstSNI TACGTA 1 cut(s) 2250
BstUI CGCG 2 cut(s) 138, 630
BstV1I GCAGC 6 cut(s) 274, 289, 1463, 1765, 1895, 2092
BstX2I RGATCY 3 cut(s) 165, 1147, 2083
BstYI RGATCY 3 cut(s) 165, 1147, 2083
BsuI GTATCC 2 cut(s) 569, 1743
BsuRI GGCC 7 cut(s) 281, 346, 514, 608, 831, 1015, 1709
BtgI CCRYGG 2 cut(s) 334, 628
BtsCI GGATG 6 cut(s) 675, 754, 1109, 1412, 1442, 2140
BtsI GCAGTG 1 cut(s) 1455
BtsIMutI CAGTG 3 cut(s) 573, 892, 1455
BtuMI TCGCGA 1 cut(s) 138
BveI ACCTGC 1 cut(s) 89
Cac8I GCNNGC 6 cut(s) 117, 136, 348, 1131, 1281, 1517
CaiI CAGNNNCTG 1 cut(s) 2161
CciI TCATGA 1 cut(s) 2175
CfoI GCGC 1 cut(s) 459
Cfr13I GGNCC 7 cut(s) 512, 606, 607, 830, 1014, 1307, 1657
Cfr42I CCGCGG 1 cut(s) 631
Csp6I GTAC 7 cut(s) 102, 466, 956, 1313, 2019, 2247, 2251
CspCI CAANNNNNGTGG 2 cut(s) 1348, 1383
CviQI GTAC 7 cut(s) 102, 466, 956, 1313, 2019, 2247, 2251
DdeI CTNAG 3 cut(s) 206, 435, 576
DpnI GATC 9 cut(s) 167, 183, 1028, 1149, 1358, 1425, 1431, 1757, 2085
DpnII GATC 9 cut(s) 165, 181, 1026, 1147, 1356, 1423, 1429, 1755, 2083
Eam1104I CTCTTC 1 cut(s) 105
EarI CTCTTC 1 cut(s) 105
Ecl136II GAGCTC 1 cut(s) 1613
Eco105I TACGTA 1 cut(s) 2250
Eco130I CCWWGG 2 cut(s) 334, 384
Eco147I AGGCCT 1 cut(s) 1709
Eco24I GRGCYC 3 cut(s) 610, 1615, 2233
Eco32I GATATC 2 cut(s) 413, 1344
Eco47I GGWCC 2 cut(s) 1307, 1657
Eco53kI GAGCTC 1 cut(s) 1613
EcoICRI GAGCTC 1 cut(s) 1613
EcoNI CCTNNNNNAGG 1 cut(s) 295
EcoO109I RGGNCCY 3 cut(s) 512, 606, 1657
EcoRII CCWGG 2 cut(s) 665, 1347
EcoRV GATATC 2 cut(s) 413, 1344
EcoT14I CCWWGG 2 cut(s) 334, 384
EcoT38I GRGCYC 3 cut(s) 610, 1615, 2233
ErhI CCWWGG 2 cut(s) 334, 384
FalI AAGNNNNNCTT 4 cut(s) 437, 469, 1263, 1295
FaqI GGGAC 1 cut(s) 986
FblI GTMKAC 1 cut(s) 2068
Fnu4HI GCNGC 7 cut(s) 132, 288, 303, 1477, 1779, 1884, 2106
FokI GGATG 6 cut(s) 682, 741, 1116, 1419, 1449, 2147
FriOI GRGCYC 3 cut(s) 610, 1615, 2233
Fsp4HI GCNGC 7 cut(s) 132, 288, 303, 1477, 1779, 1884, 2106
FspBI CTAG 4 cut(s) 516, 1152, 1182, 1379
GlaI GCGC 1 cut(s) 458
GluI GCNGC 7 cut(s) 132, 288, 303, 1477, 1779, 1884, 2106
GsaI CCCAGC 1 cut(s) 1287
GsuI CTGGAG 3 cut(s) 222, 1629, 2277
HaeIII GGCC 7 cut(s) 281, 346, 514, 608, 831, 1015, 1709
HapII CCGG 2 cut(s) 610, 719
HhaI GCGC 1 cut(s) 459
Hin6I GCGC 1 cut(s) 457
HinP1I GCGC 1 cut(s) 457
HincII GTYRAC 3 cut(s) 786, 1930, 2069
HindII GTYRAC 3 cut(s) 786, 1930, 2069
HindIII AAGCTT 2 cut(s) 1161, 1272
HinfI GANTC 6 cut(s) 619, 1218, 1225, 1288, 1573, 1702
HpaI GTTAAC 1 cut(s) 786
HpaII CCGG 2 cut(s) 610, 719
Hpy166II GTNNAC 8 cut(s) 104, 528, 638, 786, 878, 1689, 1930, 2069
Hpy188I TCNGA 8 cut(s) 201, 477, 566, 1031, 1230, 1423, 1450, 2045
Hpy8I GTNNAC 8 cut(s) 104, 528, 638, 786, 878, 1689, 1930, 2069
Hpy99I CGWCG 1 cut(s) 1685
HpyAV CCTTC 7 cut(s) 248, 319, 439, 563, 692, 945, 1740
HpyCH4IV ACGT 3 cut(s) 961, 1680, 2249
HpyF10VI GCNNNNNNNGC 4 cut(s) 70, 287, 579, 1280
HpyF3I CTNAG 3 cut(s) 206, 435, 576
HpySE526I ACGT 3 cut(s) 961, 1680, 2249
HspAI GCGC 1 cut(s) 457
KspAI GTTAAC 1 cut(s) 786
KspI CCGCGG 1 cut(s) 631
Kzo9I GATC 9 cut(s) 165, 181, 1026, 1147, 1356, 1423, 1429, 1755, 2083
LguI GCTCTTC 1 cut(s) 105
LmnI GCTCC 4 cut(s) 1600, 1610, 1618, 2258
Lsp1109I GCAGC 6 cut(s) 274, 289, 1463, 1765, 1895, 2092
LweI GCATC 6 cut(s) 192, 543, 1116, 1183, 1397, 1534
MaeI CTAG 4 cut(s) 516, 1152, 1182, 1379
MaeII ACGT 3 cut(s) 961, 1680, 2249
MaeIII GTNAC 5 cut(s) 1370, 1391, 1497, 1563, 2190
MalI GATC 9 cut(s) 167, 183, 1028, 1149, 1358, 1425, 1431, 1757, 2085
MbiI CCGCTC 1 cut(s) 134
MboI GATC 9 cut(s) 165, 181, 1026, 1147, 1356, 1423, 1429, 1755, 2083
MfeI CAATTG 2 cut(s) 854, 1903
MflI RGATCY 3 cut(s) 165, 1147, 2083
MhlI GDGCHC 6 cut(s) 610, 640, 1615, 1691, 1977, 2233
MlyI GAGTC 1 cut(s) 1582
MmeI TCCRAC 3 cut(s) 1605, 2111, 2112
MroXI GAANNNNTTC 1 cut(s) 2216
MseI TTAA 8 cut(s) 417, 785, 894, 1020, 1121, 1167, 1317, 2048
MslI CAYNNNNRTG 4 cut(s) 477, 918, 990, 1471
MspA1I CMGCKG 2 cut(s) 287, 630
MspCI CTTAAG 1 cut(s) 1316
MspI CCGG 2 cut(s) 610, 719
MspR9I CCNGG 4 cut(s) 610, 667, 719, 1349
MunI CAATTG 2 cut(s) 854, 1903
Mva1269I GAATGC 2 cut(s) 322, 2188
MvaI CCWGG 2 cut(s) 667, 1349
MvnI CGCG 2 cut(s) 138, 630
MwoI GCNNNNNNNGC 4 cut(s) 70, 287, 579, 1280
NciI CCSGG 2 cut(s) 610, 719
NcoI CCATGG 1 cut(s) 334
NdeII GATC 9 cut(s) 165, 181, 1026, 1147, 1356, 1423, 1429, 1755, 2083
NlaIV GGNNCC 3 cut(s) 608, 1149, 1602
NmeAIII GCCGAG 1 cut(s) 1555
NmuCI GTSAC 4 cut(s) 1370, 1391, 1497, 1563
NruI TCGCGA 1 cut(s) 138
NspI RCATGY 1 cut(s) 537
PagI TCATGA 1 cut(s) 2175
PceI AGGCCT 1 cut(s) 1709
PciI ACATGT 1 cut(s) 533
PciSI GCTCTTC 1 cut(s) 105
PctI GAATGC 2 cut(s) 322, 2188
PdmI GAANNNNTTC 1 cut(s) 2216
PfeI GAWTC 5 cut(s) 619, 1218, 1225, 1288, 1702
Pfl23II CGTACG 1 cut(s) 2246
PflFI GACNNNGTC 1 cut(s) 1661
PflMI CCANNNNNTGG 1 cut(s) 1583
PkrI GCNGC 7 cut(s) 133, 289, 304, 1478, 1780, 1885, 2107
PleI GAGTC 1 cut(s) 1581
PpsI GAGTC 1 cut(s) 1581
Ppu21I YACGTR 1 cut(s) 2250
PpuMI RGGWCCY 1 cut(s) 1657
PscI ACATGT 1 cut(s) 533
PshBI ATTAAT 2 cut(s) 1121, 2048
Psp124BI GAGCTC 1 cut(s) 1615
Psp1406I AACGTT 1 cut(s) 961
Psp5II RGGWCCY 1 cut(s) 1657
Psp6I CCWGG 2 cut(s) 665, 1347
PspFI CCCAGC 1 cut(s) 1283
PspGI CCWGG 2 cut(s) 665, 1347
PspLI CGTACG 1 cut(s) 2246
PspN4I GGNNCC 3 cut(s) 608, 1149, 1602
PspOMI GGGCCC 1 cut(s) 606
PspPI GGNCC 7 cut(s) 512, 606, 607, 830, 1014, 1307, 1657
PspPPI RGGWCCY 1 cut(s) 1657
PsrI GAACNNNNNNTAC 4 cut(s) 1073, 1105, 1985, 2017
PstI CTGCAG 2 cut(s) 1462, 1783
PstNI CAGNNNCTG 1 cut(s) 2161
PsuI RGATCY 3 cut(s) 165, 1147, 2083
PsyI GACNNNGTC 1 cut(s) 1661
PvuII CAGCTG 1 cut(s) 287
RruI TCGCGA 1 cut(s) 138
RsaI GTAC 7 cut(s) 103, 467, 957, 1314, 2020, 2248, 2252
RsaNI GTAC 7 cut(s) 102, 466, 956, 1313, 2019, 2247, 2251
RseI CAYNNNNRTG 4 cut(s) 477, 918, 990, 1471
SacI GAGCTC 1 cut(s) 1615
SacII CCGCGG 1 cut(s) 631
SalI GTCGAC 1 cut(s) 2067
SapI GCTCTTC 1 cut(s) 105
SaqAI TTAA 8 cut(s) 417, 785, 894, 1020, 1121, 1167, 1317, 2048
SatI GCNGC 7 cut(s) 132, 288, 303, 1477, 1779, 1884, 2106
Sau3AI GATC 9 cut(s) 165, 181, 1026, 1147, 1356, 1423, 1429, 1755, 2083
Sau96I GGNCC 7 cut(s) 512, 606, 607, 830, 1014, 1307, 1657
ScaI AGTACT 1 cut(s) 1314
SchI GAGTC 1 cut(s) 1582
ScrFI CCNGG 4 cut(s) 610, 667, 719, 1349
SduI GDGCHC 6 cut(s) 610, 640, 1615, 1691, 1977, 2233
SfaNI GCATC 6 cut(s) 192, 543, 1116, 1183, 1397, 1534
SfcI CTRYAG 5 cut(s) 292, 778, 1458, 1568, 1779
Sfr303I CCGCGG 1 cut(s) 631
SgrBI CCGCGG 1 cut(s) 631
SinI GGWCC 2 cut(s) 1307, 1657
SmiMI CAYNNNNRTG 4 cut(s) 477, 918, 990, 1471
SmlI CTYRAG 2 cut(s) 1316, 2010
SmoI CTYRAG 2 cut(s) 1316, 2010
SnaBI TACGTA 1 cut(s) 2250
SseBI AGGCCT 1 cut(s) 1709
SsiI CCGC 5 cut(s) 132, 582, 628, 630, 1155
SspI AATATT 1 cut(s) 644
SspMI CTAG 4 cut(s) 516, 1152, 1182, 1379
SstI GAGCTC 1 cut(s) 1615
StuI AGGCCT 1 cut(s) 1709
StyD4I CCNGG 4 cut(s) 608, 665, 717, 1347
StyI CCWWGG 2 cut(s) 334, 384
TaiI ACGT 3 cut(s) 964, 1683, 2252
TaqI TCGA 6 cut(s) 586, 1087, 1110, 1683, 1700, 2068
TatI WGTACW 2 cut(s) 1312, 2018
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 5 cut(s) 619, 1218, 1225, 1288, 1702
Tru1I TTAA 8 cut(s) 417, 785, 894, 1020, 1121, 1167, 1317, 2048
Tru9I TTAA 8 cut(s) 417, 785, 894, 1020, 1121, 1167, 1317, 2048
TscAI CASTG 3 cut(s) 580, 892, 1462
TseFI GTSAC 4 cut(s) 1370, 1391, 1497, 1563
TseI GCWGC 6 cut(s) 287, 302, 1476, 1778, 1883, 2105
Tsp45I GTSAC 4 cut(s) 1370, 1391, 1497, 1563
TspDTI ATGAA 9 cut(s) 495, 642, 743, 1086, 1314, 1683, 1756, 2135, 2164
TspGWI ACGGA 1 cut(s) 1379
TspRI CASTG 3 cut(s) 580, 892, 1462
Tth111I GACNNNGTC 1 cut(s) 1661
Van91I CCANNNNNTGG 1 cut(s) 1583
Vha464I CTTAAG 1 cut(s) 1316
VneI GTGCAC 2 cut(s) 636, 1687
VpaK11BI GGWCC 2 cut(s) 1307, 1657
VspI ATTAAT 2 cut(s) 1121, 2048
XagI CCTNNNNNAGG 1 cut(s) 295
XapI RAATTY 7 cut(s) 231, 355, 367, 821, 867, 1301, 1646
XceI RCATGY 1 cut(s) 537
XcmI CCANNNNNNNNNTGG 1 cut(s) 1301
XmiI GTMKAC 1 cut(s) 2068
XmnI GAANNNNTTC 1 cut(s) 2216
XspI CTAG 4 cut(s) 516, 1152, 1182, 1379
ZrmI AGTACT 1 cut(s) 1314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.