Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0000173

Physical Location & Seq

Genomic Coordinates Forward (+)

52886312 .. 52894153

Gene Structure 7842 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG371800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2313 bp

ATGTGGAAGCTTAAGGTTGCAGAAGGTGGGGATTCCTCAGCTTACATTTACAGCACAAACAACTTTGTCGGAAGACAGACATGGGAGTTTGATCCTGACGCAGGAACTGCTGAAGAGCGAGCTGAGGTTGAAGAGGCCCGTCTTCATTTCTACAACAACCGCTATCAGGTCAAGCCCAGTGGTGACCTTCTCTGGCGTATACAGTTCCTAAGGGAGAAGAAGTTCAAACAGACAATTCCTCCCGTAAAAATTGAAGATGGCGAGGTTATCACACATGAAAAAACCACAGATGCACTGAGAAGGTCTATCCACTTCTTCTCAGCGTTGCAGGCAAGCGATGGCCATTGGCCTGCGGAAAATGCCGGCCCTTTGTTTTTCCTTCCTCCCTTGGTCATATGTATGTACATTACTGGGCATCTTAACAGTGTATTCCCTGAAGAGCATCGCAAAGAAATTCTGCGTTACATATACTATCATCAGAATGAAGATGGTGGTTGGGGACTACACGTCGAAGGTCACAGCACCATGTTCAGCACAGCTCTCAGCTACATTTGTATGCGTATTCTCGGAGAAGGACCTGATGATGGTGGCCAAGACAATGCTTGTCCAAGAGCAAGAAAGTGGATTCTTGATCATGGTGGTGTCACACACATACCCTCTTGGGGAAAGACTTGGCTTTCGATACTTGGTTTGTTTGATTGGTCTGGAAGCAACCCTATGCCCCCAGAGTTTTGGATTCTTCCTTCGTTTCTTCCTATGCATCCGGCAAAAATGTGGTGCTATTGTAGGATGGTTTACATGCCAATGTCATACCTATATGGAAAAAGGTTTGTTGGCCCAATTACACCTCTCATTCTTCAATTGAGGGAGGAACTTTTTATTCAACCTTACAATGAAATCAATTGGAAGAAAGTACGCCATCGATGTGCCAAAGAGGACATCTACTATCCTCATCCTTGGATACAGGATCTCCTTTGGGATAGCCTCTACATTTCCACAGAGCATATTCTTACTCGTTGGCCCTTCAACAAGTTGATCAGAGAAAAGGCTCTTCAAGTGACGATGAAGCATATTCATTACGAAGATGAGAACAGTCGATACATTACCATTGCGTGTGTGGAAAAGGTGTTATGTATGCTTGCCTGTTGGGTTGAAGATCCAAATGGGGACTATTTCAAGAAGCATCTGGCAAGGGTCCCGGATTATTTATGGGTTGCTGAAGATGGAATGAAGATGCAAAGTTTTGGCAGTCAGGAGTGGGATACTGGTTTTGCCATTCAAGCTTTGCTTGCTACTAATCTAACGGACGAAATTGGACCAACGCTCGCTAAAGGACATGACTTCATAAAGAAATCTCAGGTCAAAGACAACCCATCTGGTGACTTCAAAAGCATGCACCGCCACATTTCCAAAGGATCGTGGACTTTCTCCGATCAAGATCATGGATGGCAAGTTTCTGATTGCACAGCAGAAGGTTTGAAGTGTTGCCTTCTATTGTCGATGATGCCATCGGAGATAGTTGGTGAAAAAATGGAACCTGAGCGCTTATACGATTCTGTCAATGTTCTACTTTCCCTACAGAGCAAGAATGGTGGTTTATCAGCCTGGGAACCAGCAGGGGCAGCAGAGTGGTTAGAACTGTTAAATCCGACAGAGTTCTTCTCAGACATAGTAATTGAGCATGAATATGTTGAGTGCACTTCATCTGCAATCCAGGCTTTAGTACTATTTAAGAAGCTACACCCTGGACACAGGAAGAAAGAGATTGAACATTTCATCACCAATGCGATACACTACCTTGAAAATATACAAATGCCGGATGGTTCATGGTATGGAAACTGGGGAGTTTGCTTCACATATGGTACCTGGTTTGCAGTAGGAGGATTGGCAGCAGCTGGCAAGACTTTCAGCACTTGTGCAGCCATGCGCAAAGCGGTTAGTTTTCTCCTCACAACGCAGAGAGAGAATGGTGGTTGGGGAGAGAGCTATCTTTCATGTCCGCAAAAGACATACATTCCTCTCGAAGGAAATCGATCCAATTTAGTACATACTGCTTGGGCTATGATGGGTCTCATTCATGCGGGACAAGCAGAAAGAGACCCAACACCTCTTCATCGTGCAGCAAAGTTTATAATTAATTCTCAACTGGAAAATGGTGATTTTCCCCAGCAGGAAATCACTGGAGTCTTCCTGAAGAACTGCATGCTACATTATGCAGCTTATAGAAATATATACCCACTCTGGGCTCTTGCAGAATACCGCAAGCGGGTTCCATTGCCTTCCAAGGCCAGATTTGTTCACCCTATTATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006629 GO:0006720 GO:0006721 GO:0006722 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009987 GO:0016104 GO:0016114 GO:0016853 GO:0016866 GO:0019742 GO:0019745 GO:0031559 GO:0042300 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0071704 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00909 ko01110 map00909 map01110

Pfam Domains

Protein Families

SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

770

Amino Acids

88.48

Weight (kDa)

6.3

Isoelectric Point (pI)

49.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SQHop_cyclase_N	PF13249	101 - 399	5.8e-36	Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C	PF13243	419 - 755	1.9e-51	Squalene-hopene cyclase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000173)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G66960 AT1G66960 AT1G66960 AT1G66960 AT1G78950 AT1G78950 AT1G78955 AT1G78955 AT1G78955 AT1G78955 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78960 AT1G78970 AT1G78970 AT1G78970
fragaria_vesca	FvH4_5g29480 FvH4_6g36450 FvH4_6g36450 FvH4_6g36500 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g36520 FvH4_6g37620
malus_domestica	MD03G1089600.v1.1 MD09G1167700.v1.1 MD09G1168200.v1.1 MD09G1168300.v1.1 MD11G1098600.v1.1 MD17G1158300.v1.1 MD17G1158700.v1.1 MD17G1159500.v1.1 MD17G1160500.v1.1 MD17G1182500.v1.1 MD17G1182700.v1.1 MD17G1245800.v1.1 MD17G1246300.v1.1 MD17G1246600.v1.1
prunus_persica	Prupe.3G025700_v2.0.a1 Prupe.3G025700_v2.0.a1 Prupe.3G025800_v2.0.a1 Prupe.3G025900_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026200_v2.0.a1 Prupe.3G026400_v2.0.a1 Prupe.3G026500_v2.0.a1 Prupe.3G026700_v2.0.a1 Prupe.3G026800_v2.0.a1
pyrus_communis	pycom03g07120 pycom09g08520 pycom09g08590 pycom09g08610 pycom10g10070 pycom11g08310 pycom16g19880 pycom17g15190 pycom17g15300
rosa_chinensis	RchiOBHm_Chr2g0136221 RchiOBHm_Chr2g0148451 RchiOBHm_Chr2g0148501 RchiOBHm_Chr2g0148521 RchiOBHm_Chr2g0148661 RchiOBHm_Chr2g0148701 RchiOBHm_Chr2g0148811 RchiOBHm_Chr2g0148941 RchiOBHm_Chr2g0148981 RchiOBHm_Chr2g0150451 RchiOBHm_Chr2g0150521 RchiOBHm_Chr2g0150611 RchiOBHm_Chr4g0420831 RchiOBHm_Chr5g0049791 RchiOBHm_Chr5g0049801
rosa_laevigata	RLG00000007735 RLG00000020351 RLG00000020352 RLG00000020357 RLG00000020358 RLG00000020360 RLG00000029093 RLG00000030686
rosa_multiflora	Rmu_co8274513.1_g000001 Rmu_sc0000048.1_g000003 Rmu_sc0000048.1_g000033 Rmu_sc0000048.1_g000039 Rmu_sc0001397.1_g000011 Rmu_sc0001998.1_g000039 Rmu_sc0002070.1_g000055 Rmu_sc0002082.1_g000052 Rmu_sc0003181.1_g000010 Rmu_sc0005104.1_g000005 Rmu_sc0006758.1_g000002 Rmu_sc0008411.1_g000029 Rmu_sc0008411.1_g000030 Rmu_sc0017472.1_g000001 Rmu_sc0017472.1_g000003 Rmu_sc0029876.1_g000001 Rmu_ssc0000480.1_g000024 Rmu_ssc0000480.1_g000036
rosa_roxburghii	Rroxscaffold_2G00097070 Rroxscaffold_2G00098540 Rroxscaffold_2G00098560 Rroxscaffold_2G00098570 Rroxscaffold_2G00098650 Rroxscaffold_2G00098710 Rroxscaffold_2G00098740 Rroxscaffold_3G00225830 Rroxscaffold_3G00236870 Rroxscaffold_4G00312240
rosa_rugosa	Rorug01G0047000 Rorug02G0372300 Rorug02G0407200 Rorug02G0407300 Rorug02G0408400 Rorug02G0421500 Rorug07G0208900
rosa_samantha	Rh2AG380200 Rh2AG465700 Rh2AG465800 Rh2AG466800 Rh2AG467100 Rh2AG467600 Rh2AG480900 Rh2AG481400 Rh2AG481700 Rh2CG073500 Rh2CG452200 Rh2CG453200 Rh2CG453600 Rh2CG454100 Rh2CG467100 Rh2CG467800 Rh2CG468000 Rh2DG403100 Rh2DG487800 Rh2DG487900 Rh2DG488800 Rh2DG502000 Rh2DG502200 Rh2DG502300 Rh2DG503100 Rh3AG319300 Rh4AG227700 Rh4BG371800 Rh5AG327400 Rh5DG350600 Rh7AG349800
rosa_wichuraiana	Rw0G010080 Rw2G031110 Rw2G037980 Rw2G037990 Rw2G038000 Rw2G038010 Rw2G038080 Rw2G038100 Rw2G039260 Rw2G039290 Rw2G039470 Rw4G019710 Rw5G030930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2132
Acc16I	TGCGCA	1 cut(s)	1928
Acc65I	GGTACC	1 cut(s)	1862
AccB1I	GGYRCC	1 cut(s)	1862
AccI	GTMKAC	1 cut(s)	199
AciI	CCGC	8 cut(s)	160, 353, 1399, 1934, 2000, 2081, 2260, 2266
AclWI	GGATC	5 cut(s)	86, 975, 1151, 1423, 2028
AcoI	YGGCCR	2 cut(s)	340, 589
AcsI	RAATTY	1 cut(s)	453
AcuI	CTGAAG	4 cut(s)	132, 456, 1239, 2213
AfaI	GTAC	5 cut(s)	404, 915, 1725, 1864, 2046
AfeI	AGCGCT	1 cut(s)	1544
AfiI	CCNNNNNNNGG	7 cut(s)	101, 166, 584, 662, 2024, 2242, 2266
AflII	CTTAAG	1 cut(s)	11
AflIII	ACRYGT	1 cut(s)	505
AhdI	GACNNNNNGTC	1 cut(s)	506
AjiI	CACGTC	1 cut(s)	508
AjnI	CCWGG	4 cut(s)	1604, 1713, 1744, 1865
AloI	GAACNNNNNNTCC	2 cut(s)	206, 238
Alw21I	GWGCWC	1 cut(s)	1700
Alw26I	GTCTC	2 cut(s)	2075, 2091
Alw44I	GTGCAC	1 cut(s)	1696
AlwI	GGATC	5 cut(s)	86, 975, 1151, 1423, 2028
AlwNI	CAGNNNCTG	2 cut(s)	107, 1895
Aor51HI	AGCGCT	1 cut(s)	1544
AoxI	GGCC	8 cut(s)	135, 340, 347, 364, 589, 835, 1019, 2286
ApaLI	GTGCAC	1 cut(s)	1696
ApeKI	GCWGC	6 cut(s)	1622, 1889, 1892, 1919, 2120, 2216
ApoI	RAATTY	1 cut(s)	453
ArsI	GACNNNNNNTTYG	2 cut(s)	661, 693
AseI	ATTAAT	1 cut(s)	2136
Asp700I	GAANNNNTTC	1 cut(s)	221
Asp718I	GGTACC	1 cut(s)	1862
AspLEI	GCGC	2 cut(s)	1545, 1929
AspS9I	GGNCC	7 cut(s)	136, 365, 575, 836, 1020, 1195, 1316
AsuC2I	CCSGG	1 cut(s)	1199
AsuHPI	GGTGA	6 cut(s)	194, 1391, 1535, 1771, 2168, 2291
AvaII	GGWCC	3 cut(s)	575, 1195, 1316
AxyI	CCTNAGG	1 cut(s)	209
BaeGI	GKGCMC	1 cut(s)	1700
BalI	TGGCCA	2 cut(s)	342, 591
BanI	GGYRCC	1 cut(s)	1862
BanII	GRGCYC	1 cut(s)	2248
BbsI	GAAGAC	3 cut(s)	79, 134, 2179
Bbv12I	GWGCWC	1 cut(s)	1700
BbvCI	CCTCAGC	2 cut(s)	37, 123
BbvI	GCAGC	6 cut(s)	1634, 1901, 1904, 1931, 2132, 2228
BciT130I	CCWGG	4 cut(s)	1606, 1715, 1746, 1867
BciVI	GTATCC	2 cut(s)	954, 1255
BclI	TGATCA	2 cut(s)	631, 1035
BcnI	CCSGG	1 cut(s)	1199
BcoDI	GTCTC	2 cut(s)	2075, 2091
BfmI	CTRYAG	1 cut(s)	1577
BfoI	RGCGCY	1 cut(s)	1546
BfrI	CTTAAG	1 cut(s)	11
BfuI	GTATCC	2 cut(s)	954, 1255
BisI	GCNGC	6 cut(s)	1623, 1890, 1893, 1920, 2121, 2217
BlsI	GCNGC	6 cut(s)	1624, 1891, 1894, 1921, 2122, 2218
BmcAI	AGTACT	1 cut(s)	1725
Bme1390I	CCNGG	5 cut(s)	1199, 1606, 1715, 1746, 1867
Bme18I	GGWCC	3 cut(s)	575, 1195, 1316
BmeRI	GACNNNNNGTC	1 cut(s)	506
BmgBI	CACGTC	1 cut(s)	508
BmgT120I	GGNCC	7 cut(s)	136, 365, 575, 836, 1020, 1195, 1316
BmiI	GGNNCC	6 cut(s)	1196, 1197, 1536, 1611, 1864, 2271
BmrFI	CCNGG	5 cut(s)	1199, 1606, 1715, 1746, 1867
BmrI	ACTGGG	3 cut(s)	171, 420, 1849
BmsI	GCATC	7 cut(s)	280, 424, 451, 769, 1192, 1224, 1494
BmuI	ACTGGG	3 cut(s)	171, 420, 1849
BpiI	GAAGAC	3 cut(s)	79, 134, 2179
BplI	GAGNNNNNCTC	2 cut(s)	1646, 1678
BpmI	CTGGAG	1 cut(s)	2202
Bpu10I	CCTNAGC	3 cut(s)	37, 123, 1539
BpuMI	CCSGG	1 cut(s)	1199
Bsa29I	ATCGAT	2 cut(s)	922, 2032
BsaBI	GATNNNNATC	1 cut(s)	1437
BsaI	GGTCTC	2 cut(s)	2075, 2091
BsaJI	CCNNGG	5 cut(s)	387, 956, 1605, 1744, 2283
BsaXI	ACNNNNNCTCC	6 cut(s)	206, 223, 236, 253, 954, 984
Bsc4I	CCNNNNNNNGG	7 cut(s)	101, 166, 584, 662, 2024, 2242, 2266
Bse118I	RCCGGY	1 cut(s)	362
Bse1I	ACTGG	6 cut(s)	177, 415, 1270, 1844, 2151, 2185
Bse21I	CCTNAGG	1 cut(s)	209
Bse3DI	GCAATG	2 cut(s)	1107, 2273
Bse8I	GATNNNNATC	1 cut(s)	1437
BseBI	CCWGG	4 cut(s)	1606, 1715, 1746, 1867
BseCI	ATCGAT	2 cut(s)	922, 2032
BseDI	CCNNGG	5 cut(s)	387, 956, 1605, 1744, 2283
BseGI	GGATG	5 cut(s)	760, 795, 952, 1451, 1825
BseJI	GATNNNNATC	1 cut(s)	1437
BseLI	CCNNNNNNNGG	7 cut(s)	101, 166, 584, 662, 2024, 2242, 2266
BseMI	GCAATG	2 cut(s)	1107, 2273
BseMII	CTCAG	8 cut(s)	51, 114, 287, 333, 556, 1370, 1530, 1677
BseNI	ACTGG	6 cut(s)	177, 415, 1270, 1844, 2151, 2185
BseRI	GAGGAG	1 cut(s)	1937
BseSI	GKGCMC	1 cut(s)	1700
BseXI	GCAGC	6 cut(s)	1634, 1901, 1904, 1931, 2132, 2228
BseYI	CCCAGC	1 cut(s)	2166
BsgI	GTGCAG	2 cut(s)	1938, 2139
BshFI	GGCC	8 cut(s)	137, 342, 349, 366, 591, 837, 1021, 2288
BshNI	GGYRCC	1 cut(s)	1862
BshVI	ATCGAT	2 cut(s)	922, 2032
BsiHKAI	GWGCWC	1 cut(s)	1700
BsiSI	CCGG	4 cut(s)	363, 764, 1199, 1817
BslFI	GGGAC	4 cut(s)	513, 1181, 1181, 2097
BslI	CCNNNNNNNGG	7 cut(s)	101, 166, 584, 662, 2024, 2242, 2266
BsmAI	GTCTC	2 cut(s)	2075, 2091
BsmFI	GGGAC	4 cut(s)	513, 1181, 1181, 2097
BsnI	GGCC	8 cut(s)	137, 342, 349, 366, 591, 837, 1021, 2288
Bso31I	GGTCTC	2 cut(s)	2075, 2091
Bsp1286I	GDGCHC	2 cut(s)	1700, 2248
Bsp1407I	TGTACA	1 cut(s)	402
Bsp143I	GATC	9 cut(s)	91, 631, 967, 1035, 1156, 1415, 1432, 1438, 2033
BspACI	CCGC	8 cut(s)	160, 353, 1399, 1934, 2000, 2081, 2260, 2266
BspANI	GGCC	8 cut(s)	137, 342, 349, 366, 591, 837, 1021, 2288
BspCNI	CTCAG	8 cut(s)	50, 115, 288, 332, 555, 1369, 1531, 1676
BspDI	ATCGAT	2 cut(s)	922, 2032
BspLI	GGNNCC	6 cut(s)	1196, 1197, 1536, 1611, 1864, 2271
BspPI	GGATC	5 cut(s)	86, 975, 1151, 1423, 2028
BspQI	GCTCTTC	3 cut(s)	108, 432, 1056
BspT107I	GGYRCC	1 cut(s)	1862
BspTI	CTTAAG	1 cut(s)	11
BspTNI	GGTCTC	2 cut(s)	2075, 2091
BsrDI	GCAATG	2 cut(s)	1107, 2273
BsrFI	RCCGGY	1 cut(s)	362
BsrGI	TGTACA	1 cut(s)	402
BsrI	ACTGG	6 cut(s)	177, 415, 1270, 1844, 2151, 2185
BssAI	RCCGGY	1 cut(s)	362
BssECI	CCNNGG	5 cut(s)	387, 956, 1605, 1744, 2283
BssMI	GATC	9 cut(s)	91, 631, 967, 1035, 1156, 1415, 1432, 1438, 2033
BssNAI	GTATAC	1 cut(s)	200
BssT1I	CCWWGG	3 cut(s)	387, 956, 2283
Bst1107I	GTATAC	1 cut(s)	200
Bst2UI	CCWGG	4 cut(s)	1606, 1715, 1746, 1867
Bst4CI	ACNGT	4 cut(s)	204, 425, 1094, 1641
Bst6I	CTCTTC	5 cut(s)	108, 126, 432, 1056, 2115
BstAFI	CTTAAG	1 cut(s)	11
BstAPI	GCANNNNNTGC	1 cut(s)	107
BstAUI	TGTACA	1 cut(s)	402
BstDEI	CTNAG	9 cut(s)	37, 123, 209, 296, 319, 542, 1356, 1539, 1663
BstEII	GGTNACC	1 cut(s)	182
BstENI	CCTNNNNNAGG	2 cut(s)	99, 2022
BstF5I	GGATG	5 cut(s)	760, 795, 952, 1451, 1825
BstH2I	RGCGCY	1 cut(s)	1546
BstHHI	GCGC	2 cut(s)	1545, 1929
BstKTI	GATC	9 cut(s)	94, 634, 970, 1038, 1159, 1418, 1435, 1441, 2036
BstMAI	GTCTC	2 cut(s)	2075, 2091
BstMBI	GATC	9 cut(s)	91, 631, 967, 1035, 1156, 1415, 1432, 1438, 2033
BstMWI	GCNNNNNNNGC	9 cut(s)	107, 329, 359, 1280, 1289, 1398, 1622, 1715, 2087
BstNI	CCWGG	4 cut(s)	1606, 1715, 1746, 1867
BstNSI	RCATGY	3 cut(s)	802, 1396, 2206
BstPI	GGTNACC	1 cut(s)	182
BstSCI	CCNGG	5 cut(s)	1197, 1604, 1713, 1744, 1865
BstSFI	CTRYAG	1 cut(s)	1577
BstSLI	GKGCMC	1 cut(s)	1700
BstV1I	GCAGC	6 cut(s)	1634, 1901, 1904, 1931, 2132, 2228
BstV2I	GAAGAC	3 cut(s)	79, 134, 2179
BstX2I	RGATCY	2 cut(s)	967, 1156
BstXI	CCANNNNNNTGG	1 cut(s)	732
BstYI	RGATCY	2 cut(s)	967, 1156
BstZ17I	GTATAC	1 cut(s)	200
Bsu15I	ATCGAT	2 cut(s)	922, 2032
Bsu36I	CCTNAGG	1 cut(s)	209
BsuI	GTATCC	2 cut(s)	954, 1255
BsuRI	GGCC	8 cut(s)	137, 342, 349, 366, 591, 837, 1021, 2288
BsuTUI	ATCGAT	2 cut(s)	922, 2032
BtgZI	GCGATG	2 cut(s)	351, 428
BtrI	CACGTC	1 cut(s)	508
BtsCI	GGATG	5 cut(s)	760, 795, 952, 1451, 1825
BtsIMutI	CAGTG	4 cut(s)	184, 293, 430, 2178
CaiI	CAGNNNCTG	2 cut(s)	107, 1895
CfoI	GCGC	2 cut(s)	1545, 1929
Cfr10I	RCCGGY	1 cut(s)	362
Cfr13I	GGNCC	7 cut(s)	136, 365, 575, 836, 1020, 1195, 1316
ClaI	ATCGAT	2 cut(s)	922, 2032
CseI	GACGC	1 cut(s)	107
CsiI	ACCWGGT	1 cut(s)	1865
Csp6I	GTAC	5 cut(s)	403, 914, 1724, 1863, 2045
CspCI	CAANNNNNGTGG	4 cut(s)	160, 195, 1573, 1608
CviQI	GTAC	5 cut(s)	403, 914, 1724, 1863, 2045
DdeI	CTNAG	9 cut(s)	37, 123, 209, 296, 319, 542, 1356, 1539, 1663
DpnI	GATC	9 cut(s)	93, 633, 969, 1037, 1158, 1417, 1434, 1440, 2035
DpnII	GATC	9 cut(s)	91, 631, 967, 1035, 1156, 1415, 1432, 1438, 2033
DriI	GACNNNNNGTC	1 cut(s)	506
EaeI	YGGCCR	2 cut(s)	340, 589
Eam1104I	CTCTTC	5 cut(s)	108, 126, 432, 1056, 2115
Eam1105I	GACNNNNNGTC	1 cut(s)	506
EarI	CTCTTC	5 cut(s)	108, 126, 432, 1056, 2115
Eco130I	CCWWGG	3 cut(s)	387, 956, 2283
Eco24I	GRGCYC	1 cut(s)	2248
Eco31I	GGTCTC	2 cut(s)	2075, 2091
Eco47I	GGWCC	3 cut(s)	575, 1195, 1316
Eco47III	AGCGCT	1 cut(s)	1544
Eco57I	CTGAAG	4 cut(s)	132, 456, 1239, 2213
Eco81I	CCTNAGG	1 cut(s)	209
Eco91I	GGTNACC	1 cut(s)	182
EcoNI	CCTNNNNNAGG	2 cut(s)	99, 2022
EcoO109I	RGGNCCY	2 cut(s)	575, 1195
EcoO65I	GGTNACC	1 cut(s)	182
EcoRII	CCWGG	4 cut(s)	1604, 1713, 1744, 1865
EcoT14I	CCWWGG	3 cut(s)	387, 956, 2283
EcoT22I	ATGCAT	1 cut(s)	762
EcoT38I	GRGCYC	1 cut(s)	2248
ErhI	CCWWGG	3 cut(s)	387, 956, 2283
FalI	AAGNNNNNCTT	2 cut(s)	1272, 1304
FaqI	GGGAC	4 cut(s)	513, 1181, 1181, 2097
FauI	CCCGC	2 cut(s)	2074, 2259
FauNDI	CATATG	2 cut(s)	395, 1858
FbaI	TGATCA	2 cut(s)	631, 1035
FblI	GTMKAC	1 cut(s)	199
Fnu4HI	GCNGC	6 cut(s)	1623, 1890, 1893, 1920, 2121, 2217
FokI	GGATG	5 cut(s)	747, 802, 939, 1458, 1832
FriOI	GRGCYC	1 cut(s)	2248
Fsp4HI	GCNGC	6 cut(s)	1623, 1890, 1893, 1920, 2121, 2217
FspI	TGCGCA	1 cut(s)	1928
GlaI	GCGC	2 cut(s)	1544, 1928
GluI	GCNGC	6 cut(s)	1623, 1890, 1893, 1920, 2121, 2217
GsaI	CCCAGC	1 cut(s)	2170
GsuI	CTGGAG	1 cut(s)	2202
HaeII	RGCGCY	1 cut(s)	1546
HaeIII	GGCC	8 cut(s)	137, 342, 349, 366, 591, 837, 1021, 2288
HapII	CCGG	4 cut(s)	363, 764, 1199, 1817
HgaI	GACGC	1 cut(s)	107
HhaI	GCGC	2 cut(s)	1545, 1929
Hin6I	GCGC	2 cut(s)	1543, 1927
HinP1I	GCGC	2 cut(s)	1543, 1927
HindIII	AAGCTT	2 cut(s)	8, 1281
HinfI	GANTC	5 cut(s)	32, 625, 736, 1553, 2184
HpaII	CCGG	4 cut(s)	363, 764, 1199, 1817
HphI	GGTGA	6 cut(s)	194, 1391, 1535, 1771, 2168, 2291
Hpy166II	GTNNAC	5 cut(s)	200, 796, 1422, 1698, 2299
Hpy188I	TCNGA	9 cut(s)	71, 480, 569, 1040, 1432, 1459, 1513, 1650, 1666
Hpy188III	TCNNGA	8 cut(s)	95, 629, 705, 1177, 1253, 1436, 2021, 2191
Hpy8I	GTNNAC	5 cut(s)	200, 796, 1422, 1698, 2299
Hpy99I	CGWCG	1 cut(s)	512
HpyCH4III	ACNGT	4 cut(s)	204, 425, 1094, 1641
HpyCH4IV	ACGT	1 cut(s)	507
HpyF10VI	GCNNNNNNNGC	9 cut(s)	107, 329, 359, 1280, 1289, 1398, 1622, 1715, 2087
HpyF3I	CTNAG	9 cut(s)	37, 123, 209, 296, 319, 542, 1356, 1539, 1663
HpySE526I	ACGT	1 cut(s)	507
HspAI	GCGC	2 cut(s)	1543, 1927
KflI	GGGWCCC	1 cut(s)	1195
KpnI	GGTACC	1 cut(s)	1866
KroI	GCCGGC	1 cut(s)	362
KroNI	GCCGGC	1 cut(s)	364
Ksp22I	TGATCA	2 cut(s)	631, 1035
Kzo9I	GATC	9 cut(s)	91, 631, 967, 1035, 1156, 1415, 1432, 1438, 2033
LguI	GCTCTTC	3 cut(s)	108, 432, 1056
Lsp1109I	GCAGC	6 cut(s)	1634, 1901, 1904, 1931, 2132, 2228
LweI	GCATC	7 cut(s)	280, 424, 451, 769, 1192, 1224, 1494
MabI	ACCWGGT	1 cut(s)	1865
MaeII	ACGT	1 cut(s)	507
MaeIII	GTNAC	6 cut(s)	182, 461, 515, 643, 1057, 1379
MalI	GATC	9 cut(s)	93, 633, 969, 1037, 1158, 1417, 1434, 1440, 2035
MboI	GATC	9 cut(s)	91, 631, 967, 1035, 1156, 1415, 1432, 1438, 2033
MfeI	CAATTG	2 cut(s)	860, 901
MflI	RGATCY	2 cut(s)	967, 1156
MhlI	GDGCHC	2 cut(s)	1700, 2248
MlsI	TGGCCA	2 cut(s)	342, 591
MluNI	TGGCCA	2 cut(s)	342, 591
MlyI	GAGTC	1 cut(s)	2193
MmeI	TCCRAC	2 cut(s)	49, 1673
Mox20I	TGGCCA	2 cut(s)	342, 591
Mph1103I	ATGCAT	1 cut(s)	762
MroNI	GCCGGC	1 cut(s)	362
MroXI	GAANNNNTTC	1 cut(s)	221
MscI	TGGCCA	2 cut(s)	342, 591
MseI	TTAA	5 cut(s)	12, 420, 1643, 1731, 2136
MslI	CAYNNNNRTG	7 cut(s)	398, 480, 554, 639, 803, 924, 1686
Msp20I	TGGCCA	2 cut(s)	342, 591
MspA1I	CMGCKG	1 cut(s)	1895
MspCI	CTTAAG	1 cut(s)	11
MspI	CCGG	4 cut(s)	363, 764, 1199, 1817
MspR9I	CCNGG	5 cut(s)	1199, 1606, 1715, 1746, 1867
MunI	CAATTG	2 cut(s)	860, 901
MvaI	CCWGG	4 cut(s)	1606, 1715, 1746, 1867
MwoI	GCNNNNNNNGC	9 cut(s)	107, 329, 359, 1280, 1289, 1398, 1622, 1715, 2087
NaeI	GCCGGC	1 cut(s)	364
NciI	CCSGG	1 cut(s)	1199
NdeI	CATATG	2 cut(s)	395, 1858
NdeII	GATC	9 cut(s)	91, 631, 967, 1035, 1156, 1415, 1432, 1438, 2033
NgoMIV	GCCGGC	1 cut(s)	362
NlaIV	GGNNCC	6 cut(s)	1196, 1197, 1536, 1611, 1864, 2271
NmuCI	GTSAC	5 cut(s)	182, 515, 643, 1057, 1379
NsbI	TGCGCA	1 cut(s)	1928
NsiI	ATGCAT	1 cut(s)	762
NspI	RCATGY	3 cut(s)	802, 1396, 2206
PaeI	GCATGC	2 cut(s)	1396, 2206
PciSI	GCTCTTC	3 cut(s)	108, 432, 1056
PdiI	GCCGGC	1 cut(s)	364
PdmI	GAANNNNTTC	1 cut(s)	221
PfeI	GAWTC	4 cut(s)	32, 625, 736, 1553
PfoI	TCCNGGA	1 cut(s)	1197
PkrI	GCNGC	6 cut(s)	1624, 1891, 1894, 1921, 2122, 2218
PleI	GAGTC	1 cut(s)	2192
PpsI	GAGTC	1 cut(s)	2192
PpuMI	RGGWCCY	2 cut(s)	575, 1195
PshBI	ATTAAT	1 cut(s)	2136
PsiI	TTATAA	1 cut(s)	2132
Psp5II	RGGWCCY	2 cut(s)	575, 1195
Psp6I	CCWGG	4 cut(s)	1604, 1713, 1744, 1865
PspEI	GGTNACC	1 cut(s)	182
PspFI	CCCAGC	1 cut(s)	2166
PspGI	CCWGG	4 cut(s)	1604, 1713, 1744, 1865
PspN4I	GGNNCC	6 cut(s)	1196, 1197, 1536, 1611, 1864, 2271
PspPI	GGNCC	7 cut(s)	136, 365, 575, 836, 1020, 1195, 1316
PspPPI	RGGWCCY	2 cut(s)	575, 1195
PsrI	GAACNNNNNNTAC	2 cut(s)	1082, 1114
PstNI	CAGNNNCTG	2 cut(s)	107, 1895
PsuI	RGATCY	2 cut(s)	967, 1156
PvuII	CAGCTG	1 cut(s)	1895
RsaI	GTAC	5 cut(s)	404, 915, 1725, 1864, 2046
RsaNI	GTAC	5 cut(s)	403, 914, 1724, 1863, 2045
RseI	CAYNNNNRTG	7 cut(s)	398, 480, 554, 639, 803, 924, 1686
SapI	GCTCTTC	3 cut(s)	108, 432, 1056
SaqAI	TTAA	5 cut(s)	12, 420, 1643, 1731, 2136
SatI	GCNGC	6 cut(s)	1623, 1890, 1893, 1920, 2121, 2217
Sau3AI	GATC	9 cut(s)	91, 631, 967, 1035, 1156, 1415, 1432, 1438, 2033
Sau96I	GGNCC	7 cut(s)	136, 365, 575, 836, 1020, 1195, 1316
ScaI	AGTACT	1 cut(s)	1725
SchI	GAGTC	1 cut(s)	2193
ScrFI	CCNGG	5 cut(s)	1199, 1606, 1715, 1746, 1867
SduI	GDGCHC	2 cut(s)	1700, 2248
SexAI	ACCWGGT	1 cut(s)	1865
SfaNI	GCATC	7 cut(s)	280, 424, 451, 769, 1192, 1224, 1494
SfcI	CTRYAG	1 cut(s)	1577
SinI	GGWCC	3 cut(s)	575, 1195, 1316
SmiMI	CAYNNNNRTG	7 cut(s)	398, 480, 554, 639, 803, 924, 1686
SmlI	CTYRAG	1 cut(s)	11
SmoI	CTYRAG	1 cut(s)	11
SphI	GCATGC	2 cut(s)	1396, 2206
SsiI	CCGC	8 cut(s)	160, 353, 1399, 1934, 2000, 2081, 2260, 2266
StyD4I	CCNGG	5 cut(s)	1197, 1604, 1713, 1744, 1865
StyI	CCWWGG	3 cut(s)	387, 956, 2283
TaaI	ACNGT	4 cut(s)	204, 425, 1094, 1641
TaiI	ACGT	1 cut(s)	510
TaqI	TCGA	7 cut(s)	510, 680, 922, 1096, 1499, 2022, 2032
TatI	WGTACW	3 cut(s)	402, 1723, 2044
TfiI	GAWTC	4 cut(s)	32, 625, 736, 1553
Tru1I	TTAA	5 cut(s)	12, 420, 1643, 1731, 2136
Tru9I	TTAA	5 cut(s)	12, 420, 1643, 1731, 2136
TscAI	CASTG	4 cut(s)	184, 300, 430, 2185
TseFI	GTSAC	5 cut(s)	182, 515, 643, 1057, 1379
TseI	GCWGC	6 cut(s)	1622, 1889, 1892, 1919, 2120, 2216
Tsp45I	GTSAC	5 cut(s)	182, 515, 643, 1057, 1379
TspGWI	ACGGA	1 cut(s)	1319
TspRI	CASTG	4 cut(s)	184, 300, 430, 2185
Vha464I	CTTAAG	1 cut(s)	11
VneI	GTGCAC	1 cut(s)	1696
VpaK11BI	GGWCC	3 cut(s)	575, 1195, 1316
VspI	ATTAAT	1 cut(s)	2136
XagI	CCTNNNNNAGG	2 cut(s)	99, 2022
XapI	RAATTY	1 cut(s)	453
XceI	RCATGY	3 cut(s)	802, 1396, 2206
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1114
XmiI	GTMKAC	1 cut(s)	199
XmnI	GAANNNNTTC	1 cut(s)	221
ZrmI	AGTACT	1 cut(s)	1725
Zsp2I	ATGCAT	1 cut(s)	762

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.